TY  - JOUR
AU  - Paunović, Marija
AU  - Milošević, Maja
AU  - Mitrović-Ajtić, Olivera
AU  - Veličković, Nataša
AU  - Micić, Bojana
AU  - Nedić, Olgica
AU  - Todorović, Vanja
AU  - Vučić, Vesna
AU  - Petrović, Snježana
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5598
AB  - Diets high in fat and sugar lead to metabolic syndrome (MetS) and related chronic diseases. We investigated the effects of commercially available, cold-pressed polyphenol-rich black currant (BC) and cornelian cherry (CC) juices on the prevention of MetS in Wistar rats induced by a 10-weeks high-fat high-fructose (HFF) diet. Juice consumption, either BC or CC, with a HFF diet resulted in lower serum triglycerides compared to only the HFF consumption. Both juices also mitigated the effects of HFF on the liver, pancreas, and adipose tissue, by preserving liver and pancreas histomorphology and reducing visceral fat and adipocyte size. Furthermore, supplementation with both juices reduced glucagon and up-regulated insulin expression in the pancreas of the rats on the HFF diet, whereas the BC also showed improved glucose regulation. BC juice also reduced the expression of IL-6 and hepatic inflammation compared to the group only on HFF diet. Both juices, especially BC, could be a convenient solution for the prevention of MetS in humans.
PB  - Elsevier Ltd
T2  - Heliyon
T1  - Polyphenol-rich black currant and cornelian cherry juices ameliorate metabolic syndrome induced by a high-fat high-fructose diet in Wistar rats
VL  - 10
IS  - 7
DO  - 10.1016/j.heliyon.2024.e27709
ER  - 

@article{
author = "Paunović, Marija and Milošević, Maja and Mitrović-Ajtić, Olivera and Veličković, Nataša and Micić, Bojana and Nedić, Olgica and Todorović, Vanja and Vučić, Vesna and Petrović, Snježana",
year = "2024",
abstract = "Diets high in fat and sugar lead to metabolic syndrome (MetS) and related chronic diseases. We investigated the effects of commercially available, cold-pressed polyphenol-rich black currant (BC) and cornelian cherry (CC) juices on the prevention of MetS in Wistar rats induced by a 10-weeks high-fat high-fructose (HFF) diet. Juice consumption, either BC or CC, with a HFF diet resulted in lower serum triglycerides compared to only the HFF consumption. Both juices also mitigated the effects of HFF on the liver, pancreas, and adipose tissue, by preserving liver and pancreas histomorphology and reducing visceral fat and adipocyte size. Furthermore, supplementation with both juices reduced glucagon and up-regulated insulin expression in the pancreas of the rats on the HFF diet, whereas the BC also showed improved glucose regulation. BC juice also reduced the expression of IL-6 and hepatic inflammation compared to the group only on HFF diet. Both juices, especially BC, could be a convenient solution for the prevention of MetS in humans.",
publisher = "Elsevier Ltd",
journal = "Heliyon",
title = "Polyphenol-rich black currant and cornelian cherry juices ameliorate metabolic syndrome induced by a high-fat high-fructose diet in Wistar rats",
volume = "10",
number = "7",
doi = "10.1016/j.heliyon.2024.e27709"
}

Paunović, M., Milošević, M., Mitrović-Ajtić, O., Veličković, N., Micić, B., Nedić, O., Todorović, V., Vučić, V.,& Petrović, S.. (2024). Polyphenol-rich black currant and cornelian cherry juices ameliorate metabolic syndrome induced by a high-fat high-fructose diet in Wistar rats. in Heliyon
Elsevier Ltd., 10(7).
https://doi.org/10.1016/j.heliyon.2024.e27709

Paunović M, Milošević M, Mitrović-Ajtić O, Veličković N, Micić B, Nedić O, Todorović V, Vučić V, Petrović S. Polyphenol-rich black currant and cornelian cherry juices ameliorate metabolic syndrome induced by a high-fat high-fructose diet in Wistar rats. in Heliyon. 2024;10(7).
doi:10.1016/j.heliyon.2024.e27709 .

Paunović, Marija, Milošević, Maja, Mitrović-Ajtić, Olivera, Veličković, Nataša, Micić, Bojana, Nedić, Olgica, Todorović, Vanja, Vučić, Vesna, Petrović, Snježana, "Polyphenol-rich black currant and cornelian cherry juices ameliorate metabolic syndrome induced by a high-fat high-fructose diet in Wistar rats" in Heliyon, 10, no. 7 (2024),
https://doi.org/10.1016/j.heliyon.2024.e27709 . .

TY  - JOUR
AU  - Paunović, Marija
AU  - Kotur-Stevuljević, Jelena
AU  - Arsić, Aleksandra
AU  - Milošević, Maja
AU  - Todorović, Vanja
AU  - Guzonjić, Azra
AU  - Vučić, Vesna
AU  - Petrović, Snježana
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4918
AB  - A Western-style diet, rich in fat and simple sugars, is the main risk factor for a significant number of chronic diseases and disorders, as well as for a progression of metabolic syndrome (MetS). One of the key mechanisms involved in MetS development is increased oxidative stress caused by the accumulation of body fat. Some dietary polyphenols have shown a protective role in preventing oxidative-stress-induced damage. We investigated the difference in the oxidative response of plasma, liver, and visceral adipose tissue in rats fed with a high-fat high-fructose (HFF) diet for ten weeks, and the effectiveness of polyphenol-rich juices (black currant (BC) and cornelian cherry (CC)) in HFF-diet-induced oxidative stress prevention. The most prominent impact of the HFF diet on redox parameters was recorded in the liver, whereas adipose tissue showed the most potent protection mechanisms against oxidative stress. Consumption of both juices decreased advanced oxidation protein product (AOPP) level in plasma, increased paraoxonase1 (PON1) activity in the liver, and significantly decreased total oxidative status (TOS) in adipose tissue. BC exerted stronger antioxidative potential than CC and decreased the superoxide anion radical (O2•−) level in the liver. It also reduced TOS, total antioxidative status (TAS), and malondialdehyde (MDA) concentration in adipose tissue. The multiple linear regression analysis has shown that the best predictors of MetS development, estimated through the increase in visceral adiposity, were superoxide dismutase (SOD), AOPP, TOS, and TAS. The consumption of polyphenol-rich juices may provide a convenient approach for the systemic reduction of oxidative stress parameters.
PB  - MDPI
T2  - Antioxidants
T1  - Antioxidative Effects of Black Currant and Cornelian Cherry Juices in Different Tissues of an Experimental Model of Metabolic Syndrome in Rats
VL  - 12
IS  - 6
DO  - 10.3390/antiox12061148
ER  - 

@article{
author = "Paunović, Marija and Kotur-Stevuljević, Jelena and Arsić, Aleksandra and Milošević, Maja and Todorović, Vanja and Guzonjić, Azra and Vučić, Vesna and Petrović, Snježana",
year = "2023",
abstract = "A Western-style diet, rich in fat and simple sugars, is the main risk factor for a significant number of chronic diseases and disorders, as well as for a progression of metabolic syndrome (MetS). One of the key mechanisms involved in MetS development is increased oxidative stress caused by the accumulation of body fat. Some dietary polyphenols have shown a protective role in preventing oxidative-stress-induced damage. We investigated the difference in the oxidative response of plasma, liver, and visceral adipose tissue in rats fed with a high-fat high-fructose (HFF) diet for ten weeks, and the effectiveness of polyphenol-rich juices (black currant (BC) and cornelian cherry (CC)) in HFF-diet-induced oxidative stress prevention. The most prominent impact of the HFF diet on redox parameters was recorded in the liver, whereas adipose tissue showed the most potent protection mechanisms against oxidative stress. Consumption of both juices decreased advanced oxidation protein product (AOPP) level in plasma, increased paraoxonase1 (PON1) activity in the liver, and significantly decreased total oxidative status (TOS) in adipose tissue. BC exerted stronger antioxidative potential than CC and decreased the superoxide anion radical (O2•−) level in the liver. It also reduced TOS, total antioxidative status (TAS), and malondialdehyde (MDA) concentration in adipose tissue. The multiple linear regression analysis has shown that the best predictors of MetS development, estimated through the increase in visceral adiposity, were superoxide dismutase (SOD), AOPP, TOS, and TAS. The consumption of polyphenol-rich juices may provide a convenient approach for the systemic reduction of oxidative stress parameters.",
publisher = "MDPI",
journal = "Antioxidants",
title = "Antioxidative Effects of Black Currant and Cornelian Cherry Juices in Different Tissues of an Experimental Model of Metabolic Syndrome in Rats",
volume = "12",
number = "6",
doi = "10.3390/antiox12061148"
}

Paunović, M., Kotur-Stevuljević, J., Arsić, A., Milošević, M., Todorović, V., Guzonjić, A., Vučić, V.,& Petrović, S.. (2023). Antioxidative Effects of Black Currant and Cornelian Cherry Juices in Different Tissues of an Experimental Model of Metabolic Syndrome in Rats. in Antioxidants
MDPI., 12(6).
https://doi.org/10.3390/antiox12061148

Paunović M, Kotur-Stevuljević J, Arsić A, Milošević M, Todorović V, Guzonjić A, Vučić V, Petrović S. Antioxidative Effects of Black Currant and Cornelian Cherry Juices in Different Tissues of an Experimental Model of Metabolic Syndrome in Rats. in Antioxidants. 2023;12(6).
doi:10.3390/antiox12061148 .

Paunović, Marija, Kotur-Stevuljević, Jelena, Arsić, Aleksandra, Milošević, Maja, Todorović, Vanja, Guzonjić, Azra, Vučić, Vesna, Petrović, Snježana, "Antioxidative Effects of Black Currant and Cornelian Cherry Juices in Different Tissues of an Experimental Model of Metabolic Syndrome in Rats" in Antioxidants, 12, no. 6 (2023),
https://doi.org/10.3390/antiox12061148 . .

TY  - JOUR
AU  - Rajković, Katarina
AU  - Drobac, Milica
AU  - Milić, Petar
AU  - Vučić, Vesna
AU  - Arsić, Aleksandra
AU  - Perić, Mirjana
AU  - Radunović, Milena
AU  - Jeremić, Sanja
AU  - Arsenijević, Jelena
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5035
AB  - Juglans nigra (Black walnut) is a source of health-supporting biologically active compounds used in traditional medicine. The investigation of bioactive compounds in black walnut could lead to its broader application, as well as to the application of its by-products. Therefore, this study aimed to characterize J. nigra nut and green husk based on chemical analysis of their petroleum ether and ethanol extracts obtained by ultrasonic and reflux extraction methods, respectively. Different extract fractions were tested for their antimicrobial activities using Gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa), Gram-positive bacteria (Enterococcus faecalis, Staphylococcus aureus) and yeast (reference strain and clinical isolates of Candida albicans). The ethanol extracts analysis, performed by high performance liquid chromatography, singled out the ellagic acid as the most dominant compound in nut ((55.0±1.3)×10-3 kg m-3) and green husk ((114.1±0.5)×10-3 kg m-3) extracts. Non-polar compounds were evaluated using gas chromatography analysis of petroleum ether extracts. Juglans nigra nut and green husk contained two saturated fatty acids, palmitic acid (C16:0) and stearic acid (C18:0), then, monounsaturated fatty acids, palmitoleic (C16:1n-7), oleic (C18:1n-9) and vaccenic acid (C18:1n-7), as well as polyunsaturated fatty acids, linoleic (C18:2n-6), γ-linolenic (C18:3n-6) and α-linolenic (C18:3n-3) acids. Ethanol extracts of both J. nigra nut and green husk showed antimicrobial activity against C. albicans, which is the most common cause of yeast infections.
AB  - Juglans nigra (црниорах) каоизворбиолошкиактивнихједињењасекористиутра-диционалнојмедицини. Истраживањебиоактивнихједињењаприсутнихуцрноморахуможедовестидоширеприменењеговихпроизвода. ЗатоовастудијаимазациљдаокарактеришеорашастплодизеленуљускуЈ. nigra наосновухемијскеанализењиховихпетролетарскихиетанолнихекстраката, добијенихултразвучнимирефлуксметодамаекстракције. Антимикробнаактивностразличитихфракцијаекстрактајетестиранако-ришћењемГрам-негативнихбактерија (Escherichia  coli, Pseudomonas  aeruginosa), Грам- -позитивнихбактерија (Enterococcus  faecalis, Staphylococcus  aureus) иквасница (рефе-рентнисојиклиничкиизолатиCandida  albicans). Анализатечномхроматографијомвисокихперформанси, издвојилајеелагинскукиселинукаонајдоминантнијеједињењеуетанолнимекстрактимаорашастогплода ((55,0±1,3)×10-3   kg   m-3) изеленељуске((114,1±0,5)×10-3 g m-3). Неполарнаједињењасуодређенаприменомгаснехроматогра-фскеанализепетролетарскихекстракта. Орашастплодизеленаљускасадржедвезаси-ћенемаснекиселине, палмитинску (C16:0) истеаринскукиселину (C18:0), затиммоно-незасићенемаснекиселине, палмитолеинску  (C16:1n-7),  олеинску  (C18:1n-9)  ивак-ценскукиселину (C18:1n-7), каоиполинезасићенемаснекиселине, линолну (C18:2n-6), γ-линоленску (C18:3n-6) иα-линоленску (C18: 3n-3) киселину. Етанолниекстрактиора-шастогплодаизеленељускепоказалисуантимикробнуактивностнa сојевимаC.  albi-cansкојајенајчешћиузрочникгљивичнихинфекција.
PB  - Serbian Chemical Society
T2  - Journal of the Serbian Chemical Society
T1  - Chemical characterization and antimicrobial activity of Juglans nigra L. nut and green husk
T1  - Хемијска карактеризација и антимикробна активност орашастог плода и зелене љуске Juglans nigra L.
VL  - 88
IS  - 6
SP  - 603
EP  - 614
DO  - 10.2298/JSC230210024R
ER  - 

@article{
author = "Rajković, Katarina and Drobac, Milica and Milić, Petar and Vučić, Vesna and Arsić, Aleksandra and Perić, Mirjana and Radunović, Milena and Jeremić, Sanja and Arsenijević, Jelena",
year = "2023",
abstract = "Juglans nigra (Black walnut) is a source of health-supporting biologically active compounds used in traditional medicine. The investigation of bioactive compounds in black walnut could lead to its broader application, as well as to the application of its by-products. Therefore, this study aimed to characterize J. nigra nut and green husk based on chemical analysis of their petroleum ether and ethanol extracts obtained by ultrasonic and reflux extraction methods, respectively. Different extract fractions were tested for their antimicrobial activities using Gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa), Gram-positive bacteria (Enterococcus faecalis, Staphylococcus aureus) and yeast (reference strain and clinical isolates of Candida albicans). The ethanol extracts analysis, performed by high performance liquid chromatography, singled out the ellagic acid as the most dominant compound in nut ((55.0±1.3)×10-3 kg m-3) and green husk ((114.1±0.5)×10-3 kg m-3) extracts. Non-polar compounds were evaluated using gas chromatography analysis of petroleum ether extracts. Juglans nigra nut and green husk contained two saturated fatty acids, palmitic acid (C16:0) and stearic acid (C18:0), then, monounsaturated fatty acids, palmitoleic (C16:1n-7), oleic (C18:1n-9) and vaccenic acid (C18:1n-7), as well as polyunsaturated fatty acids, linoleic (C18:2n-6), γ-linolenic (C18:3n-6) and α-linolenic (C18:3n-3) acids. Ethanol extracts of both J. nigra nut and green husk showed antimicrobial activity against C. albicans, which is the most common cause of yeast infections., Juglans nigra (црниорах) каоизворбиолошкиактивнихједињењасекористиутра-диционалнојмедицини. Истраживањебиоактивнихједињењаприсутнихуцрноморахуможедовестидоширеприменењеговихпроизвода. ЗатоовастудијаимазациљдаокарактеришеорашастплодизеленуљускуЈ. nigra наосновухемијскеанализењиховихпетролетарскихиетанолнихекстраката, добијенихултразвучнимирефлуксметодамаекстракције. Антимикробнаактивностразличитихфракцијаекстрактајетестиранако-ришћењемГрам-негативнихбактерија (Escherichia  coli, Pseudomonas  aeruginosa), Грам- -позитивнихбактерија (Enterococcus  faecalis, Staphylococcus  aureus) иквасница (рефе-рентнисојиклиничкиизолатиCandida  albicans). Анализатечномхроматографијомвисокихперформанси, издвојилајеелагинскукиселинукаонајдоминантнијеједињењеуетанолнимекстрактимаорашастогплода ((55,0±1,3)×10-3   kg   m-3) изеленељуске((114,1±0,5)×10-3 g m-3). Неполарнаједињењасуодређенаприменомгаснехроматогра-фскеанализепетролетарскихекстракта. Орашастплодизеленаљускасадржедвезаси-ћенемаснекиселине, палмитинску (C16:0) истеаринскукиселину (C18:0), затиммоно-незасићенемаснекиселине, палмитолеинску  (C16:1n-7),  олеинску  (C18:1n-9)  ивак-ценскукиселину (C18:1n-7), каоиполинезасићенемаснекиселине, линолну (C18:2n-6), γ-линоленску (C18:3n-6) иα-линоленску (C18: 3n-3) киселину. Етанолниекстрактиора-шастогплодаизеленељускепоказалисуантимикробнуактивностнa сојевимаC.  albi-cansкојајенајчешћиузрочникгљивичнихинфекција.",
publisher = "Serbian Chemical Society",
journal = "Journal of the Serbian Chemical Society",
title = "Chemical characterization and antimicrobial activity of Juglans nigra L. nut and green husk, Хемијска карактеризација и антимикробна активност орашастог плода и зелене љуске Juglans nigra L.",
volume = "88",
number = "6",
pages = "603-614",
doi = "10.2298/JSC230210024R"
}

Rajković, K., Drobac, M., Milić, P., Vučić, V., Arsić, A., Perić, M., Radunović, M., Jeremić, S.,& Arsenijević, J.. (2023). Chemical characterization and antimicrobial activity of Juglans nigra L. nut and green husk. in Journal of the Serbian Chemical Society
Serbian Chemical Society., 88(6), 603-614.
https://doi.org/10.2298/JSC230210024R

Rajković K, Drobac M, Milić P, Vučić V, Arsić A, Perić M, Radunović M, Jeremić S, Arsenijević J. Chemical characterization and antimicrobial activity of Juglans nigra L. nut and green husk. in Journal of the Serbian Chemical Society. 2023;88(6):603-614.
doi:10.2298/JSC230210024R .

Rajković, Katarina, Drobac, Milica, Milić, Petar, Vučić, Vesna, Arsić, Aleksandra, Perić, Mirjana, Radunović, Milena, Jeremić, Sanja, Arsenijević, Jelena, "Chemical characterization and antimicrobial activity of Juglans nigra L. nut and green husk" in Journal of the Serbian Chemical Society, 88, no. 6 (2023):603-614,
https://doi.org/10.2298/JSC230210024R . .

TY  - JOUR
AU  - Bokan, Goran
AU  - Glamočanin, Tanja
AU  - Mavija, Zoran
AU  - Vidović, Bojana
AU  - Stojanović, Ana
AU  - Björnsson, Einar
AU  - Vučić, Vesna
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4997
AB  - Herb-induced liver injury (HILI) caused by herbal supplements, natural products, and products used in traditional medicine are important for differential diagnoses in patients with acute liver injury without an obvious etiology. The root of Withania somnifera (L.) Dunal, commonly known as ashwagandha, has been used in Ayurvedic medicine for thousands of years to promote health and longevity. Due to various biological activities, ashwagandha and its extracts became widespread as herbal supplements on the global market. Although it is generally considered safe, there are several reported cases of ashwagandha-related liver injury, and one case ended with liver transplantation. In this paper, we review all reported cases so far. Additionally, we describe two new cases of ashwagandha hepatotoxicity. In the first case, a 36-year-old man used ashwagandha capsules (450 mg, three times daily) for 6 months before he developed nausea, pruritus, and dark-colored urine. In the second case, a 30-year-old woman developed pruritus after 45 days of using ashwagandha capsules (450 mg). In both cases, serum bilirubin and liver enzymes (aspartate transaminase (AST), alanine transaminase (ALT), and alkaline phosphatase (ALP) were increased. The liver injury pattern was hepatocellular (R-value 11.1) and mixed (R-value 2.6), respectively. The updated Roussel Uclaf Causality Assessment Method (RUCAM) (both cases with a score of seven) indicated a “probable” relationship with ashwagandha. Clinical and liver function improvements were observed after the discontinuation of ashwagandha supplement use. By increasing the data related to ashwagandha-induced liver injury, these reports support that consuming ashwagandha supplements is not without its safety concerns.
PB  - MDPI
T2  - Pharmaceuticals
T1  - Herb-Induced Liver Injury by Ayurvedic Ashwagandha as Assessed for Causality by the Updated RUCAM: An Emerging Cause
VL  - 16
IS  - 8
DO  - 10.3390/ph16081129
ER  - 

@article{
author = "Bokan, Goran and Glamočanin, Tanja and Mavija, Zoran and Vidović, Bojana and Stojanović, Ana and Björnsson, Einar and Vučić, Vesna",
year = "2023",
abstract = "Herb-induced liver injury (HILI) caused by herbal supplements, natural products, and products used in traditional medicine are important for differential diagnoses in patients with acute liver injury without an obvious etiology. The root of Withania somnifera (L.) Dunal, commonly known as ashwagandha, has been used in Ayurvedic medicine for thousands of years to promote health and longevity. Due to various biological activities, ashwagandha and its extracts became widespread as herbal supplements on the global market. Although it is generally considered safe, there are several reported cases of ashwagandha-related liver injury, and one case ended with liver transplantation. In this paper, we review all reported cases so far. Additionally, we describe two new cases of ashwagandha hepatotoxicity. In the first case, a 36-year-old man used ashwagandha capsules (450 mg, three times daily) for 6 months before he developed nausea, pruritus, and dark-colored urine. In the second case, a 30-year-old woman developed pruritus after 45 days of using ashwagandha capsules (450 mg). In both cases, serum bilirubin and liver enzymes (aspartate transaminase (AST), alanine transaminase (ALT), and alkaline phosphatase (ALP) were increased. The liver injury pattern was hepatocellular (R-value 11.1) and mixed (R-value 2.6), respectively. The updated Roussel Uclaf Causality Assessment Method (RUCAM) (both cases with a score of seven) indicated a “probable” relationship with ashwagandha. Clinical and liver function improvements were observed after the discontinuation of ashwagandha supplement use. By increasing the data related to ashwagandha-induced liver injury, these reports support that consuming ashwagandha supplements is not without its safety concerns.",
publisher = "MDPI",
journal = "Pharmaceuticals",
title = "Herb-Induced Liver Injury by Ayurvedic Ashwagandha as Assessed for Causality by the Updated RUCAM: An Emerging Cause",
volume = "16",
number = "8",
doi = "10.3390/ph16081129"
}

Bokan, G., Glamočanin, T., Mavija, Z., Vidović, B., Stojanović, A., Björnsson, E.,& Vučić, V.. (2023). Herb-Induced Liver Injury by Ayurvedic Ashwagandha as Assessed for Causality by the Updated RUCAM: An Emerging Cause. in Pharmaceuticals
MDPI., 16(8).
https://doi.org/10.3390/ph16081129

Bokan G, Glamočanin T, Mavija Z, Vidović B, Stojanović A, Björnsson E, Vučić V. Herb-Induced Liver Injury by Ayurvedic Ashwagandha as Assessed for Causality by the Updated RUCAM: An Emerging Cause. in Pharmaceuticals. 2023;16(8).
doi:10.3390/ph16081129 .

Bokan, Goran, Glamočanin, Tanja, Mavija, Zoran, Vidović, Bojana, Stojanović, Ana, Björnsson, Einar, Vučić, Vesna, "Herb-Induced Liver Injury by Ayurvedic Ashwagandha as Assessed for Causality by the Updated RUCAM: An Emerging Cause" in Pharmaceuticals, 16, no. 8 (2023),
https://doi.org/10.3390/ph16081129 . .