TY  - JOUR
AU  - Arsenijević, Jelena
AU  - Drobac, Milica
AU  - Šoštarić, Ivan
AU  - Jevdović, Radosav
AU  - Živković, Jelena
AU  - Ražić, Slavica
AU  - Moravcević, Đorđe
AU  - Maksimović, Zoran
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3318
AB  - In the present research, the chemical composition of aerial parts of cultivated citral chemotype of Thymus pannonicus All. (Lamiaceae), growing at different soil conditions was studied. Two plant lineages were unfertilized or treated using either nitrogen or phosphorus fertilizers at different concentration levels. Essential oil (EO) content, EO composition, and composition of hydromethanol extracts (MEs) were compared to the results obtained for wild growing plants in order to evaluate the potential of T. pannonicus for its industrial production. EO content (%, V/m) in cultivated samples varied between 0.32-0.75% and 0.62-1.05% within two examined lineages, whereas the herbs from the natural habitat contained 0.49-1.29% of EO. GC-FID/MS analysis showed that citral, i.e. mixture of isomers geranial and neral, was the most abundant constituent in all EOs (51.90-81.96%). HPLC analysis revealed that total caffeic acid derivatives, with rosmarinic acid (RA) as the most abundant compound in all extracts, as well as total apigenin glycosides contents were significantly higher in the extracts originating from the cultivated herbs (112.15-184.94 mg/g and 5.08-15.29 mg/g, respectively), in comparison to the extracts of the herbs from the natural habitats (67.61-98.75 mg/g and 1.17-1.32 mg/g). In general, the composition of cultivated herbs varied less than that of samples originating from natural habitats, indicating that controlled cultivation of citral chemotype of T. pannonicus can provide herbal drug with favourable characteristics. Only minor differences were observed between cultivated samples treated with different fertilizers.
PB  - Elsevier Science BV, Amsterdam
T2  - Industrial Crops and Products
T1  - Comparison of essential oils and hydromethanol extracts of cultivated and wild growing Thymus pannonicus All.
VL  - 130
SP  - 162
EP  - 169
DO  - 10.1016/j.indcrop.2018.12.055
ER  - 

@article{
author = "Arsenijević, Jelena and Drobac, Milica and Šoštarić, Ivan and Jevdović, Radosav and Živković, Jelena and Ražić, Slavica and Moravcević, Đorđe and Maksimović, Zoran",
year = "2019",
abstract = "In the present research, the chemical composition of aerial parts of cultivated citral chemotype of Thymus pannonicus All. (Lamiaceae), growing at different soil conditions was studied. Two plant lineages were unfertilized or treated using either nitrogen or phosphorus fertilizers at different concentration levels. Essential oil (EO) content, EO composition, and composition of hydromethanol extracts (MEs) were compared to the results obtained for wild growing plants in order to evaluate the potential of T. pannonicus for its industrial production. EO content (%, V/m) in cultivated samples varied between 0.32-0.75% and 0.62-1.05% within two examined lineages, whereas the herbs from the natural habitat contained 0.49-1.29% of EO. GC-FID/MS analysis showed that citral, i.e. mixture of isomers geranial and neral, was the most abundant constituent in all EOs (51.90-81.96%). HPLC analysis revealed that total caffeic acid derivatives, with rosmarinic acid (RA) as the most abundant compound in all extracts, as well as total apigenin glycosides contents were significantly higher in the extracts originating from the cultivated herbs (112.15-184.94 mg/g and 5.08-15.29 mg/g, respectively), in comparison to the extracts of the herbs from the natural habitats (67.61-98.75 mg/g and 1.17-1.32 mg/g). In general, the composition of cultivated herbs varied less than that of samples originating from natural habitats, indicating that controlled cultivation of citral chemotype of T. pannonicus can provide herbal drug with favourable characteristics. Only minor differences were observed between cultivated samples treated with different fertilizers.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Industrial Crops and Products",
title = "Comparison of essential oils and hydromethanol extracts of cultivated and wild growing Thymus pannonicus All.",
volume = "130",
pages = "162-169",
doi = "10.1016/j.indcrop.2018.12.055"
}

Arsenijević, J., Drobac, M., Šoštarić, I., Jevdović, R., Živković, J., Ražić, S., Moravcević, Đ.,& Maksimović, Z.. (2019). Comparison of essential oils and hydromethanol extracts of cultivated and wild growing Thymus pannonicus All.. in Industrial Crops and Products
Elsevier Science BV, Amsterdam., 130, 162-169.
https://doi.org/10.1016/j.indcrop.2018.12.055

Arsenijević J, Drobac M, Šoštarić I, Jevdović R, Živković J, Ražić S, Moravcević Đ, Maksimović Z. Comparison of essential oils and hydromethanol extracts of cultivated and wild growing Thymus pannonicus All.. in Industrial Crops and Products. 2019;130:162-169.
doi:10.1016/j.indcrop.2018.12.055 .

Arsenijević, Jelena, Drobac, Milica, Šoštarić, Ivan, Jevdović, Radosav, Živković, Jelena, Ražić, Slavica, Moravcević, Đorđe, Maksimović, Zoran, "Comparison of essential oils and hydromethanol extracts of cultivated and wild growing Thymus pannonicus All." in Industrial Crops and Products, 130 (2019):162-169,
https://doi.org/10.1016/j.indcrop.2018.12.055 . .

TY  - JOUR
AU  - Cebović, Tatjana
AU  - Arsenijević, Jelena
AU  - Drobac, Milica
AU  - Živković, Jelena
AU  - Šoštarić, Ivan
AU  - Maksimović, Zoran
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3158
AB  - Deodorised water extracts of aromatic plants are obtained as by-products of essential oil isolation and usually discarded as waste. However, phytochemical composition of these extracts encourages their further utilization as food additives or functional food ingredients. In this study we investigated phytochemical composition, antioxidant and in vivo antiproliferative activity of deodorised water extract of Thymus pannonicus All. (DWE). HPLC analysis revealed rosmarinic acid (RA) (71.11 +/- 1.54 mg/g) as the most abundant constituent of the extract, followed by salvianolic acid H (14.83 +/- 0.79 mg/g, calculated as RA). DWE exhibited pronounced antioxidant activity in vitro, in FRAP and DPPH tests (FRAP value: 7.41 mmol Fe/g and SC50: 3.80 mu g/g, respectively). Using the model of Ehrlich carcinoma cells in mice that were treated with DWE prior, at the time, and after tumour cells implantation, the tumour growth suppression and redox status of malignant cells (i.e., activities of antioxidant enzymes, level of glutathione and intensity of lipid peroxidation) were followed. DWE applied as pretreatment caused disturbance of antioxidant equilibrium as well as apoptosis/necrosis of up to 90% EAC cells. Results obtained in the present study revealed chemopreventive potential and possibility of T. pannonicus DWE usage. High content of RA and other phenolic compounds explains, at least in part, the observed effects.
PB  - Springer India, New Delhi
T2  - Journal of Food Science and Technology-Mysore
T1  - Potential use of deodorised water extracts: polyphenol-rich extract of Thymus pannonicus All. as a chemopreventive agent
VL  - 55
IS  - 2
SP  - 560
EP  - 567
DO  - 10.1007/s13197-017-2965-1
ER  - 

@article{
author = "Cebović, Tatjana and Arsenijević, Jelena and Drobac, Milica and Živković, Jelena and Šoštarić, Ivan and Maksimović, Zoran",
year = "2018",
abstract = "Deodorised water extracts of aromatic plants are obtained as by-products of essential oil isolation and usually discarded as waste. However, phytochemical composition of these extracts encourages their further utilization as food additives or functional food ingredients. In this study we investigated phytochemical composition, antioxidant and in vivo antiproliferative activity of deodorised water extract of Thymus pannonicus All. (DWE). HPLC analysis revealed rosmarinic acid (RA) (71.11 +/- 1.54 mg/g) as the most abundant constituent of the extract, followed by salvianolic acid H (14.83 +/- 0.79 mg/g, calculated as RA). DWE exhibited pronounced antioxidant activity in vitro, in FRAP and DPPH tests (FRAP value: 7.41 mmol Fe/g and SC50: 3.80 mu g/g, respectively). Using the model of Ehrlich carcinoma cells in mice that were treated with DWE prior, at the time, and after tumour cells implantation, the tumour growth suppression and redox status of malignant cells (i.e., activities of antioxidant enzymes, level of glutathione and intensity of lipid peroxidation) were followed. DWE applied as pretreatment caused disturbance of antioxidant equilibrium as well as apoptosis/necrosis of up to 90% EAC cells. Results obtained in the present study revealed chemopreventive potential and possibility of T. pannonicus DWE usage. High content of RA and other phenolic compounds explains, at least in part, the observed effects.",
publisher = "Springer India, New Delhi",
journal = "Journal of Food Science and Technology-Mysore",
title = "Potential use of deodorised water extracts: polyphenol-rich extract of Thymus pannonicus All. as a chemopreventive agent",
volume = "55",
number = "2",
pages = "560-567",
doi = "10.1007/s13197-017-2965-1"
}

Cebović, T., Arsenijević, J., Drobac, M., Živković, J., Šoštarić, I.,& Maksimović, Z.. (2018). Potential use of deodorised water extracts: polyphenol-rich extract of Thymus pannonicus All. as a chemopreventive agent. in Journal of Food Science and Technology-Mysore
Springer India, New Delhi., 55(2), 560-567.
https://doi.org/10.1007/s13197-017-2965-1

Cebović T, Arsenijević J, Drobac M, Živković J, Šoštarić I, Maksimović Z. Potential use of deodorised water extracts: polyphenol-rich extract of Thymus pannonicus All. as a chemopreventive agent. in Journal of Food Science and Technology-Mysore. 2018;55(2):560-567.
doi:10.1007/s13197-017-2965-1 .

Cebović, Tatjana, Arsenijević, Jelena, Drobac, Milica, Živković, Jelena, Šoštarić, Ivan, Maksimović, Zoran, "Potential use of deodorised water extracts: polyphenol-rich extract of Thymus pannonicus All. as a chemopreventive agent" in Journal of Food Science and Technology-Mysore, 55, no. 2 (2018):560-567,
https://doi.org/10.1007/s13197-017-2965-1 . .

TY  - JOUR
AU  - Ignjatović, Đurđica
AU  - Živković, Jelena
AU  - Tovilović, Gordana
AU  - Šavikin, Katarina
AU  - Tomić, Mirko
AU  - Maksimović, Zoran
AU  - Janković, Teodora
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2393
AB  - Methanol and 70% acetone extracts from Veronica jacquinii, Veronica urticifolia and Veronica teucrium were evaluated regarding their neuroprotective/antioxidant effects on the human neuroblastoma SH-SY5Y cell line. All extracts exhibited modest protective activity by increasing cell survival in cells stressed with sodium nitroprusside (9-12%) and hydrogen peroxide (16-21%) compared to non-treated cells. These activities were accompanied by reductions in the amount of superoxide radicals and index of lipid peroxidation. Extracts were further analysed for the content of total phenolics, phenylpropanoid glycosides and iridoids, and the major compounds acteoside and aucubin. The highest amount of total phenolics was observed in V. jacquinii, while V. teucrium was richest in total iridoids, acteoside and aucubin. Angiogenic properties on EA.hy926 human endothelial cells were also examined. At the highest non-toxic concentration (25 mu g/ml), the tested extracts inhibited spontaneous tube formation of endothelial cells on the extracellular matrix, implying their possible antiangiogenic activity. The most potent inhibitory effects were shown by methanol extract of V. jacquinii and aqueous acetone extract of V. teucrium. The extracts did not significantly change the adhesive or migratory capacity of EA.hy926 cells. Considering the traditional use of Veronica species, these results suggest a need for further assessment of their supposed wound-healing properties.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Frontiers in Life Science
T1  - Evaluation of angiogenic and neuroprotective potential of different extracts from three Veronica species
VL  - 8
IS  - 2
SP  - 107
EP  - 116
DO  - 10.1080/21553769.2014.998297
ER  - 

@article{
author = "Ignjatović, Đurđica and Živković, Jelena and Tovilović, Gordana and Šavikin, Katarina and Tomić, Mirko and Maksimović, Zoran and Janković, Teodora",
year = "2015",
abstract = "Methanol and 70% acetone extracts from Veronica jacquinii, Veronica urticifolia and Veronica teucrium were evaluated regarding their neuroprotective/antioxidant effects on the human neuroblastoma SH-SY5Y cell line. All extracts exhibited modest protective activity by increasing cell survival in cells stressed with sodium nitroprusside (9-12%) and hydrogen peroxide (16-21%) compared to non-treated cells. These activities were accompanied by reductions in the amount of superoxide radicals and index of lipid peroxidation. Extracts were further analysed for the content of total phenolics, phenylpropanoid glycosides and iridoids, and the major compounds acteoside and aucubin. The highest amount of total phenolics was observed in V. jacquinii, while V. teucrium was richest in total iridoids, acteoside and aucubin. Angiogenic properties on EA.hy926 human endothelial cells were also examined. At the highest non-toxic concentration (25 mu g/ml), the tested extracts inhibited spontaneous tube formation of endothelial cells on the extracellular matrix, implying their possible antiangiogenic activity. The most potent inhibitory effects were shown by methanol extract of V. jacquinii and aqueous acetone extract of V. teucrium. The extracts did not significantly change the adhesive or migratory capacity of EA.hy926 cells. Considering the traditional use of Veronica species, these results suggest a need for further assessment of their supposed wound-healing properties.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Frontiers in Life Science",
title = "Evaluation of angiogenic and neuroprotective potential of different extracts from three Veronica species",
volume = "8",
number = "2",
pages = "107-116",
doi = "10.1080/21553769.2014.998297"
}

Ignjatović, Đ., Živković, J., Tovilović, G., Šavikin, K., Tomić, M., Maksimović, Z.,& Janković, T.. (2015). Evaluation of angiogenic and neuroprotective potential of different extracts from three Veronica species. in Frontiers in Life Science
Taylor & Francis Ltd, Abingdon., 8(2), 107-116.
https://doi.org/10.1080/21553769.2014.998297

Ignjatović Đ, Živković J, Tovilović G, Šavikin K, Tomić M, Maksimović Z, Janković T. Evaluation of angiogenic and neuroprotective potential of different extracts from three Veronica species. in Frontiers in Life Science. 2015;8(2):107-116.
doi:10.1080/21553769.2014.998297 .

Ignjatović, Đurđica, Živković, Jelena, Tovilović, Gordana, Šavikin, Katarina, Tomić, Mirko, Maksimović, Zoran, Janković, Teodora, "Evaluation of angiogenic and neuroprotective potential of different extracts from three Veronica species" in Frontiers in Life Science, 8, no. 2 (2015):107-116,
https://doi.org/10.1080/21553769.2014.998297 . .

TY  - JOUR
AU  - Beara, Ivana
AU  - Živković, Jelena
AU  - Lesjak, Marija
AU  - Ristić, Jelena
AU  - Šavikin, Katarina
AU  - Maksimović, Zoran
AU  - Janković, Teodora
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2377
AB  - Aerial parts of some Veronica species are used as traditional medicine for treatment of various inflammatory conditions, including rheumatism. In the present study, phenolic content and anti-inflammatory activity of three Veronica species (Plantaginaceae), Veronica jacquinii Baumg., Veronica teucrium L. and Veronica urticifolia Jacq was studied. The identification and quantification of 30 phenolic compounds in aqueous-acetone extracts were performed using LC-MS/MS technique, and the main components were baicalin, hyperoside, isoquercetine, chlorogenic acid and quinic acid. Genistein and baicalein were detected for the first time in Plantaginaceae family and Veronica genus, respectively. The anti-inflammatory potential of tested extracts was determined using calcium ionophore-stimulated human platelets as a source of cyclooxygenase-1 (COX-1) and 12-lipoxygenase (12-LOX) enzymes, and highly sensitive and specific LC-MS/MS technique was performed for detection of the main arachidonic acid metabolites formed by them. V. urticifolia extract exerted the strongest COX-1 inhibition (IC50  lt  0.4 mg/mL), while V. jacquinii extract exhibited the highest ability to inhibit 12-LOX activity (IC50 = 1.072 mg/mL). The results obtained in this study may be used to promote better use of the genus Veronica as anti-inflammatory agents.
PB  - Elsevier Science BV, Amsterdam
T2  - Industrial Crops and Products
T1  - Phenolic profile and anti-inflammatory activity of three Veronica species
VL  - 63
SP  - 276
EP  - 280
DO  - 10.1016/j.indcrop.2014.09.034
ER  - 

@article{
author = "Beara, Ivana and Živković, Jelena and Lesjak, Marija and Ristić, Jelena and Šavikin, Katarina and Maksimović, Zoran and Janković, Teodora",
year = "2015",
abstract = "Aerial parts of some Veronica species are used as traditional medicine for treatment of various inflammatory conditions, including rheumatism. In the present study, phenolic content and anti-inflammatory activity of three Veronica species (Plantaginaceae), Veronica jacquinii Baumg., Veronica teucrium L. and Veronica urticifolia Jacq was studied. The identification and quantification of 30 phenolic compounds in aqueous-acetone extracts were performed using LC-MS/MS technique, and the main components were baicalin, hyperoside, isoquercetine, chlorogenic acid and quinic acid. Genistein and baicalein were detected for the first time in Plantaginaceae family and Veronica genus, respectively. The anti-inflammatory potential of tested extracts was determined using calcium ionophore-stimulated human platelets as a source of cyclooxygenase-1 (COX-1) and 12-lipoxygenase (12-LOX) enzymes, and highly sensitive and specific LC-MS/MS technique was performed for detection of the main arachidonic acid metabolites formed by them. V. urticifolia extract exerted the strongest COX-1 inhibition (IC50  lt  0.4 mg/mL), while V. jacquinii extract exhibited the highest ability to inhibit 12-LOX activity (IC50 = 1.072 mg/mL). The results obtained in this study may be used to promote better use of the genus Veronica as anti-inflammatory agents.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Industrial Crops and Products",
title = "Phenolic profile and anti-inflammatory activity of three Veronica species",
volume = "63",
pages = "276-280",
doi = "10.1016/j.indcrop.2014.09.034"
}

Beara, I., Živković, J., Lesjak, M., Ristić, J., Šavikin, K., Maksimović, Z.,& Janković, T.. (2015). Phenolic profile and anti-inflammatory activity of three Veronica species. in Industrial Crops and Products
Elsevier Science BV, Amsterdam., 63, 276-280.
https://doi.org/10.1016/j.indcrop.2014.09.034

Beara I, Živković J, Lesjak M, Ristić J, Šavikin K, Maksimović Z, Janković T. Phenolic profile and anti-inflammatory activity of three Veronica species. in Industrial Crops and Products. 2015;63:276-280.
doi:10.1016/j.indcrop.2014.09.034 .

Beara, Ivana, Živković, Jelena, Lesjak, Marija, Ristić, Jelena, Šavikin, Katarina, Maksimović, Zoran, Janković, Teodora, "Phenolic profile and anti-inflammatory activity of three Veronica species" in Industrial Crops and Products, 63 (2015):276-280,
https://doi.org/10.1016/j.indcrop.2014.09.034 . .

TY  - JOUR
AU  - Živković, Jelena
AU  - Barreira, Joao C. M.
AU  - Stojković, Dejan
AU  - Cebović, Tatjana
AU  - Santos-Buelga, Celestino
AU  - Maksimović, Zoran
AU  - Ferreira, Isabel C. F. R.
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2211
AB  - The phenolic profile and antibacterial, antimutagenic and antitumour activities of the methanolic extracts of Veronica urticifolia Jacq. were evaluated. HPLC-DAD/ESI-MS analysis revealed the presence of phenolic acids, flavonoids and phenylethanoids, with acteoside as the main component (14.9 mg/g of extract). Antibacterial effect was determined using the microbroth dilution assay and Staphylococcus aureus was the most sensitive strain (MIC and MBC = 7.5 mg/mL). Antimutagenic activity was evaluated by Ames mutagenicity assay. At 1 mg/plate, the tested extract afforded high protection against the mutagenicity of nitroquinoline-N-oxide (4NQO) to Salmonella typhimurium strain TA100 (inhibition rate 48.3%). Antitumour activity was screened in Ehrlich ascites carcinoma (EAC) model. Pretreatment with 2 mg/kg body weight showed statistically significant decrease in tumour cell viability, while ascites volume and tumour cell count became slightly decreased, but not to a statistically significant extent. The results indicate that V. urticifolia deserves further research into its potential chemoprevention effects.
PB  - Elsevier Science BV, Amsterdam
T2  - Journal of Functional Foods
T1  - Phenolic profile, antibacterial, antimutagenic and antitumour evaluation of Veronica urticifolia Jacq.
VL  - 9
SP  - 192
EP  - 201
DO  - 10.1016/j.jff.2014.04.024
ER  - 

@article{
author = "Živković, Jelena and Barreira, Joao C. M. and Stojković, Dejan and Cebović, Tatjana and Santos-Buelga, Celestino and Maksimović, Zoran and Ferreira, Isabel C. F. R.",
year = "2014",
abstract = "The phenolic profile and antibacterial, antimutagenic and antitumour activities of the methanolic extracts of Veronica urticifolia Jacq. were evaluated. HPLC-DAD/ESI-MS analysis revealed the presence of phenolic acids, flavonoids and phenylethanoids, with acteoside as the main component (14.9 mg/g of extract). Antibacterial effect was determined using the microbroth dilution assay and Staphylococcus aureus was the most sensitive strain (MIC and MBC = 7.5 mg/mL). Antimutagenic activity was evaluated by Ames mutagenicity assay. At 1 mg/plate, the tested extract afforded high protection against the mutagenicity of nitroquinoline-N-oxide (4NQO) to Salmonella typhimurium strain TA100 (inhibition rate 48.3%). Antitumour activity was screened in Ehrlich ascites carcinoma (EAC) model. Pretreatment with 2 mg/kg body weight showed statistically significant decrease in tumour cell viability, while ascites volume and tumour cell count became slightly decreased, but not to a statistically significant extent. The results indicate that V. urticifolia deserves further research into its potential chemoprevention effects.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Journal of Functional Foods",
title = "Phenolic profile, antibacterial, antimutagenic and antitumour evaluation of Veronica urticifolia Jacq.",
volume = "9",
pages = "192-201",
doi = "10.1016/j.jff.2014.04.024"
}

Živković, J., Barreira, J. C. M., Stojković, D., Cebović, T., Santos-Buelga, C., Maksimović, Z.,& Ferreira, I. C. F. R.. (2014). Phenolic profile, antibacterial, antimutagenic and antitumour evaluation of Veronica urticifolia Jacq.. in Journal of Functional Foods
Elsevier Science BV, Amsterdam., 9, 192-201.
https://doi.org/10.1016/j.jff.2014.04.024

Živković J, Barreira JCM, Stojković D, Cebović T, Santos-Buelga C, Maksimović Z, Ferreira ICFR. Phenolic profile, antibacterial, antimutagenic and antitumour evaluation of Veronica urticifolia Jacq.. in Journal of Functional Foods. 2014;9:192-201.
doi:10.1016/j.jff.2014.04.024 .

Živković, Jelena, Barreira, Joao C. M., Stojković, Dejan, Cebović, Tatjana, Santos-Buelga, Celestino, Maksimović, Zoran, Ferreira, Isabel C. F. R., "Phenolic profile, antibacterial, antimutagenic and antitumour evaluation of Veronica urticifolia Jacq." in Journal of Functional Foods, 9 (2014):192-201,
https://doi.org/10.1016/j.jff.2014.04.024 . .

TY  - JOUR
AU  - Živković, Jelena
AU  - Janković, Teodora
AU  - Maksimović, Zoran
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2011
AB  - In the present study, methanolic extracts of three Veronica species, namely Veronica montana, Veronica beccabunga and Veronica polita, were analyzed for the total iridoids, total phenylethanoids and total phenolics contents. The results showed that iridoid glycosides are the major class of tested secondary metabolites in selected Veronica species. The highest iridoid content (467.33 mg/g d.w.) was recorded in extract of V. polita, while the richest source of total phenolics and total phenylethanoids was the extract of V. montana (105.24 mg GAE/g d.w. and 16.03 mg acteoside/g d.w., respectively). Additionally, aucubin determination in tested extracts was performed by high-performance liquid chromatography (HPLC) and high-performance thin layer chromatography (HPTLC). The aucubin content in investigated samples determined by HPLC varied between 5.66 in extract of V. beccabunga and 69.96 mg/g d.w. in extract of V. polita. No significant differences were observed between the aucubin content in plant extracts obtained by HPLC and HPTLC techniques. The proposed HPTLC method is simple, rapid and more economical as compared to HPLC method, and thus it may be suitable for routine identification and quantification of aucubin in Veronica species.
AB  - U ovom radu, primenom spektrofotometrijskih metoda, određen je sadržaj ukupnih fenola, fenilpropanoida i iridoida u metanolnim ekstraktima herbi Veronica montana, V. beccabunga i V. polita. Rezultati spektofotometrijske analize pokazuju da su iridoidni glukozidi dominantna grupa sekundarnih metabolita u odabranim vrstama. Njihov sadržaj bio je najveći u herbi V. polita, dok je V. montana bila najbogatija ukupnim fenolima i ukupnim fenilpropanoidima. Dodatno, u ispitivanim ekstraktima kvantifikovan je aukubin primenom tehnika visoko-efikasne tečne hromatografije (HPLC) i visoko-efikasne tankoslojne hromatografije (HPTLC). Sadržaj aukubina određen primenom HPLC tehnike kretao se od 5,66 u ekstraktu V. beccabunga do 69,96 mg/g suve mase u ekstraktu V. polita. Iz rezultata dobijenih u ovom radu uočava se da nema statistički značajnih razlika između vrednosti za sadržaj aukubina dobijenih primenom HPLC i HPTLC tehnika. Predložena HPTLC metoda kao jednostavnija, ekonomičnija, sa mogućnošću analize više uzoraka istovremeno, može biti pogodna za rutinsku identifikaciju i kvantifikaciju aukubina u Veronica vrstama, kao i sastavni deo protokola za procenu autentičnosti biljnog materijala.
PB  - Institut za proučavanje lekovitog bilja Dr Josif Pančić, Beograd
T2  - Lekovite sirovine
T1  - Determination of aucubin by HPLC and HPTLC methods in methanolic extracts of Veronica montana, Veronica beccabunga and Veronica polita
T1  - Određivanje aukubina primenom HPLC i HPTLC tehnika u metanolnim ekstraktima vrsta Veronica montana, Veronica beccabunga i Veronica polita
IS  - 33
SP  - 53
EP  - 61
UR  - https://hdl.handle.net/21.15107/rcub_farfar_2011
ER  - 

@article{
author = "Živković, Jelena and Janković, Teodora and Maksimović, Zoran",
year = "2013",
abstract = "In the present study, methanolic extracts of three Veronica species, namely Veronica montana, Veronica beccabunga and Veronica polita, were analyzed for the total iridoids, total phenylethanoids and total phenolics contents. The results showed that iridoid glycosides are the major class of tested secondary metabolites in selected Veronica species. The highest iridoid content (467.33 mg/g d.w.) was recorded in extract of V. polita, while the richest source of total phenolics and total phenylethanoids was the extract of V. montana (105.24 mg GAE/g d.w. and 16.03 mg acteoside/g d.w., respectively). Additionally, aucubin determination in tested extracts was performed by high-performance liquid chromatography (HPLC) and high-performance thin layer chromatography (HPTLC). The aucubin content in investigated samples determined by HPLC varied between 5.66 in extract of V. beccabunga and 69.96 mg/g d.w. in extract of V. polita. No significant differences were observed between the aucubin content in plant extracts obtained by HPLC and HPTLC techniques. The proposed HPTLC method is simple, rapid and more economical as compared to HPLC method, and thus it may be suitable for routine identification and quantification of aucubin in Veronica species., U ovom radu, primenom spektrofotometrijskih metoda, određen je sadržaj ukupnih fenola, fenilpropanoida i iridoida u metanolnim ekstraktima herbi Veronica montana, V. beccabunga i V. polita. Rezultati spektofotometrijske analize pokazuju da su iridoidni glukozidi dominantna grupa sekundarnih metabolita u odabranim vrstama. Njihov sadržaj bio je najveći u herbi V. polita, dok je V. montana bila najbogatija ukupnim fenolima i ukupnim fenilpropanoidima. Dodatno, u ispitivanim ekstraktima kvantifikovan je aukubin primenom tehnika visoko-efikasne tečne hromatografije (HPLC) i visoko-efikasne tankoslojne hromatografije (HPTLC). Sadržaj aukubina određen primenom HPLC tehnike kretao se od 5,66 u ekstraktu V. beccabunga do 69,96 mg/g suve mase u ekstraktu V. polita. Iz rezultata dobijenih u ovom radu uočava se da nema statistički značajnih razlika između vrednosti za sadržaj aukubina dobijenih primenom HPLC i HPTLC tehnika. Predložena HPTLC metoda kao jednostavnija, ekonomičnija, sa mogućnošću analize više uzoraka istovremeno, može biti pogodna za rutinsku identifikaciju i kvantifikaciju aukubina u Veronica vrstama, kao i sastavni deo protokola za procenu autentičnosti biljnog materijala.",
publisher = "Institut za proučavanje lekovitog bilja Dr Josif Pančić, Beograd",
journal = "Lekovite sirovine",
title = "Determination of aucubin by HPLC and HPTLC methods in methanolic extracts of Veronica montana, Veronica beccabunga and Veronica polita, Određivanje aukubina primenom HPLC i HPTLC tehnika u metanolnim ekstraktima vrsta Veronica montana, Veronica beccabunga i Veronica polita",
number = "33",
pages = "53-61",
url = "https://hdl.handle.net/21.15107/rcub_farfar_2011"
}

Živković, J., Janković, T.,& Maksimović, Z.. (2013). Determination of aucubin by HPLC and HPTLC methods in methanolic extracts of Veronica montana, Veronica beccabunga and Veronica polita. in Lekovite sirovine
Institut za proučavanje lekovitog bilja Dr Josif Pančić, Beograd.(33), 53-61.
https://hdl.handle.net/21.15107/rcub_farfar_2011

Živković J, Janković T, Maksimović Z. Determination of aucubin by HPLC and HPTLC methods in methanolic extracts of Veronica montana, Veronica beccabunga and Veronica polita. in Lekovite sirovine. 2013;(33):53-61.
https://hdl.handle.net/21.15107/rcub_farfar_2011 .

Živković, Jelena, Janković, Teodora, Maksimović, Zoran, "Determination of aucubin by HPLC and HPTLC methods in methanolic extracts of Veronica montana, Veronica beccabunga and Veronica polita" in Lekovite sirovine, no. 33 (2013):53-61,
https://hdl.handle.net/21.15107/rcub_farfar_2011 .

TY  - JOUR
AU  - Stojković, Dejan
AU  - Živković, Jelena
AU  - Soković, Marina
AU  - Glamočlija, Jasmina
AU  - Ferreira, Isabel C. F. R.
AU  - Janković, Teodora
AU  - Maksimović, Zoran
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1935
AB  - This study was designed to evaluate the antibacterial activity of the Veronica montana L. water extract and its main phenolic compound, protocatechuic acid. The antibacterial activity was determined by microdilution assay against six strains of Gram-positive and Gram-negative bacteria. Listeria monocytogenes was the most sensitive of the tested bacterial species. Antibacterial preserving properties of protocatechuic acid were also evaluated after its incorporation in cream cheese, using L. monocytogenes as commonly cheese contaminant. The compound successfully inhibited L monocytogenes development in cream cheese, at room temperature and in refrigerator (25 degrees C and 4 degrees C, respectively), after 3 days of inoculation. Sensory evaluation was carried out in order to validate the mentioned food system. A possible mode of action of the tested compound towards bacterial cells was assessed and appears to be direct lysis of pathogenic cytoplasmic membrane. Prediction of pharmacokinetic properties was also performed using computational analyzes. The obtained results can serve as an important platform for the development of effective natural preservatives.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Food and Chemical Toxicology
T1  - Antibacterial activity of Veronica montana L. extract and of protocatechuic acid incorporated in a food system
VL  - 55
SP  - 209
EP  - 213
DO  - 10.1016/j.fct.2013.01.005
ER  - 

@article{
author = "Stojković, Dejan and Živković, Jelena and Soković, Marina and Glamočlija, Jasmina and Ferreira, Isabel C. F. R. and Janković, Teodora and Maksimović, Zoran",
year = "2013",
abstract = "This study was designed to evaluate the antibacterial activity of the Veronica montana L. water extract and its main phenolic compound, protocatechuic acid. The antibacterial activity was determined by microdilution assay against six strains of Gram-positive and Gram-negative bacteria. Listeria monocytogenes was the most sensitive of the tested bacterial species. Antibacterial preserving properties of protocatechuic acid were also evaluated after its incorporation in cream cheese, using L. monocytogenes as commonly cheese contaminant. The compound successfully inhibited L monocytogenes development in cream cheese, at room temperature and in refrigerator (25 degrees C and 4 degrees C, respectively), after 3 days of inoculation. Sensory evaluation was carried out in order to validate the mentioned food system. A possible mode of action of the tested compound towards bacterial cells was assessed and appears to be direct lysis of pathogenic cytoplasmic membrane. Prediction of pharmacokinetic properties was also performed using computational analyzes. The obtained results can serve as an important platform for the development of effective natural preservatives.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Food and Chemical Toxicology",
title = "Antibacterial activity of Veronica montana L. extract and of protocatechuic acid incorporated in a food system",
volume = "55",
pages = "209-213",
doi = "10.1016/j.fct.2013.01.005"
}

Stojković, D., Živković, J., Soković, M., Glamočlija, J., Ferreira, I. C. F. R., Janković, T.,& Maksimović, Z.. (2013). Antibacterial activity of Veronica montana L. extract and of protocatechuic acid incorporated in a food system. in Food and Chemical Toxicology
Pergamon-Elsevier Science Ltd, Oxford., 55, 209-213.
https://doi.org/10.1016/j.fct.2013.01.005

Stojković D, Živković J, Soković M, Glamočlija J, Ferreira ICFR, Janković T, Maksimović Z. Antibacterial activity of Veronica montana L. extract and of protocatechuic acid incorporated in a food system. in Food and Chemical Toxicology. 2013;55:209-213.
doi:10.1016/j.fct.2013.01.005 .

Stojković, Dejan, Živković, Jelena, Soković, Marina, Glamočlija, Jasmina, Ferreira, Isabel C. F. R., Janković, Teodora, Maksimović, Zoran, "Antibacterial activity of Veronica montana L. extract and of protocatechuic acid incorporated in a food system" in Food and Chemical Toxicology, 55 (2013):209-213,
https://doi.org/10.1016/j.fct.2013.01.005 . .

TY  - JOUR
AU  - Živković, Jelena
AU  - Ražić, Slavica
AU  - Arsenijević, Jelena
AU  - Maksimović, Zoran
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1833
AB  - This paper describes the relationships between the concentrations of selected trace elements in soil and their bioaccumulation in the aerial parts of three Veronica species (Plantaginaceae). Plant and soil samples were collected from three mountainous areas in Serbia, prepared by microwave-assisted acid digestion and analyzed by flame and flameless atomic absorption spectrometry. The total concentrations of Cu, Zn, Mn, Fe and Cr in the soil varied from 12.38 to 47.77, 62.78 to 138.00, 517.58 to 1675.78, 13574.22 to 35920.00 and 36.18 to 115.15 mg kg-1, respectively, while those in the plants ranged from 6.04 to 12.8, 27.66 to 58.01, 25.38 to 89.25, 35.53 to 563.26 and 0.44 to 18.96 mg kg-1, respectively. There were no significant differences in heavy metal concentrations between the tested Veronica species from the same location, indicating that their heavy metal uptake pattern was not species specific. In the case of Mn, despite its wide variation in the soil, the concentrations in the plant samples were uniform, which suggests the potential ability of the tested species to control Mn uptake and/or its translocation to the upper plant parts. Additionally, the lowest concentrations of Cu were obtained in plant samples collected from soil with the highest Fe concentrations, indicating that Cu availability to plants might be reduced due to high Fe contents in soil solution.
AB  - U ovom radu prikazan je odnos sadržaja odabranih elemenata u tragovima u zemljištu i njihove bioakumulacije u nadzemnim delovima tri Veronica vrste (Plantaginaceae). Biljni i uzorci zemljišta sakupljeni su sa tri planinska lokaliteta u Srbiji, podvrgnuti su mikrotalasnoj digestiji u kiseloj sredini i analizirani primenom plamene i elektrotermalne atomske apsorpcione spektroskopije. Ukupna koncentracija Cu, Zn, Mn, Fe i Cr u uzorcima zemljišta varira od 12,38 do 47,77, 62,78 do 138,00, 517,58 do 1675,78, 13574,22 do 35920,00 i 36,18 do 115,15 mg kg-1, dok se u biljnim uzorcima kreće u opsegu od 6,04 do 12,8, 27,66 do 58,01, 25,38 do 89,25, 35,53 do 563,26 i 0,44 do 18,96 mg kg-1, redom. Nisu pokazane značajne razlike u koncentraciji teških metala između testiranih Veronica vrsta sa istih lokaliteta, što pokazuje da model usvajanja teških metala nije karakteristika vrste. Uprkos velikih variranja koncentracije Mn u zemljištu, koncentracija u biljnim uzorcima je uniformna, što ukazuje na potencijalnu mogućnost testiranih vrsta da kontrolišu preuzimanje Mn ili njegov transport do nadzemnih delova biljke. Najniža koncentracija Cu dobijena je u biljnim uzorcima sakupljenim na zemljištu sa najvećom Fe koncentracijom, sugerišući da je dostupnost Cu biljkama redukovana usled visokog sadržaja Fe u zemljišnom rastvoru.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Heavy metal contents in Veronica species and soil from mountainous areas in Serbia
T1  - Sposobnost akumulacije teških metala Veronica vrsta sa planinskih lokaliteta u Srbiji
VL  - 77
IS  - 7
SP  - 959
EP  - 970
DO  - 10.2298/JSC111225221Z
ER  - 

@article{
author = "Živković, Jelena and Ražić, Slavica and Arsenijević, Jelena and Maksimović, Zoran",
year = "2012",
abstract = "This paper describes the relationships between the concentrations of selected trace elements in soil and their bioaccumulation in the aerial parts of three Veronica species (Plantaginaceae). Plant and soil samples were collected from three mountainous areas in Serbia, prepared by microwave-assisted acid digestion and analyzed by flame and flameless atomic absorption spectrometry. The total concentrations of Cu, Zn, Mn, Fe and Cr in the soil varied from 12.38 to 47.77, 62.78 to 138.00, 517.58 to 1675.78, 13574.22 to 35920.00 and 36.18 to 115.15 mg kg-1, respectively, while those in the plants ranged from 6.04 to 12.8, 27.66 to 58.01, 25.38 to 89.25, 35.53 to 563.26 and 0.44 to 18.96 mg kg-1, respectively. There were no significant differences in heavy metal concentrations between the tested Veronica species from the same location, indicating that their heavy metal uptake pattern was not species specific. In the case of Mn, despite its wide variation in the soil, the concentrations in the plant samples were uniform, which suggests the potential ability of the tested species to control Mn uptake and/or its translocation to the upper plant parts. Additionally, the lowest concentrations of Cu were obtained in plant samples collected from soil with the highest Fe concentrations, indicating that Cu availability to plants might be reduced due to high Fe contents in soil solution., U ovom radu prikazan je odnos sadržaja odabranih elemenata u tragovima u zemljištu i njihove bioakumulacije u nadzemnim delovima tri Veronica vrste (Plantaginaceae). Biljni i uzorci zemljišta sakupljeni su sa tri planinska lokaliteta u Srbiji, podvrgnuti su mikrotalasnoj digestiji u kiseloj sredini i analizirani primenom plamene i elektrotermalne atomske apsorpcione spektroskopije. Ukupna koncentracija Cu, Zn, Mn, Fe i Cr u uzorcima zemljišta varira od 12,38 do 47,77, 62,78 do 138,00, 517,58 do 1675,78, 13574,22 do 35920,00 i 36,18 do 115,15 mg kg-1, dok se u biljnim uzorcima kreće u opsegu od 6,04 do 12,8, 27,66 do 58,01, 25,38 do 89,25, 35,53 do 563,26 i 0,44 do 18,96 mg kg-1, redom. Nisu pokazane značajne razlike u koncentraciji teških metala između testiranih Veronica vrsta sa istih lokaliteta, što pokazuje da model usvajanja teških metala nije karakteristika vrste. Uprkos velikih variranja koncentracije Mn u zemljištu, koncentracija u biljnim uzorcima je uniformna, što ukazuje na potencijalnu mogućnost testiranih vrsta da kontrolišu preuzimanje Mn ili njegov transport do nadzemnih delova biljke. Najniža koncentracija Cu dobijena je u biljnim uzorcima sakupljenim na zemljištu sa najvećom Fe koncentracijom, sugerišući da je dostupnost Cu biljkama redukovana usled visokog sadržaja Fe u zemljišnom rastvoru.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Heavy metal contents in Veronica species and soil from mountainous areas in Serbia, Sposobnost akumulacije teških metala Veronica vrsta sa planinskih lokaliteta u Srbiji",
volume = "77",
number = "7",
pages = "959-970",
doi = "10.2298/JSC111225221Z"
}

Živković, J., Ražić, S., Arsenijević, J.,& Maksimović, Z.. (2012). Heavy metal contents in Veronica species and soil from mountainous areas in Serbia. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 77(7), 959-970.
https://doi.org/10.2298/JSC111225221Z

Živković J, Ražić S, Arsenijević J, Maksimović Z. Heavy metal contents in Veronica species and soil from mountainous areas in Serbia. in Journal of the Serbian Chemical Society. 2012;77(7):959-970.
doi:10.2298/JSC111225221Z .

Živković, Jelena, Ražić, Slavica, Arsenijević, Jelena, Maksimović, Zoran, "Heavy metal contents in Veronica species and soil from mountainous areas in Serbia" in Journal of the Serbian Chemical Society, 77, no. 7 (2012):959-970,
https://doi.org/10.2298/JSC111225221Z . .

TY  - JOUR
AU  - Živković, Jelena
AU  - Cebović, Tatjana
AU  - Maksimović, Zoran
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1707
AB  - The aim of the present study was to examine the antioxidant activity of three Veronica species (Plantaginaceae). The antioxidant potential of various extracts obtained from aerial flowering parts was evaluated by DPPH-free (1,1-diphenyl-2-picrylhydrazyl-free) radical scavenging activity and ferric-reducing antioxidant power assays. Considerable antioxidant activity was observed in the plant samples (FRAP values ranged from 0.97 to 4.85 mmol Fe2+/g, and DPPH IC50 values from 12.58 to 66.34 A mu g/ml); however, these levels were lower than the activity of the control compound butylated hydroxytoluene (BHT) (FRAP: 10.58 mmol Fe2+/g; DPPH IC50: 9.57 A mu g/ml). Also, the in vivo antioxidant effects were evaluated in several hepatic antioxidant systems in rats (activities of glutathione peroxidase, glutathione reductase, peroxidase, catalase, xanthine oxidase, glutathione content and level of thiobarbituric acid reactive substances) after treatment with different Veronica extracts, or in combination with carbon tetrachloride (CCl4). Pretreatment with 100 mg/kg b.w. of Veronica extracts inhibited CCl4-induced liver injury by decreasing TBA-RS level, increasing GSH content, and bringing the activities of CAT and Px to control levels. The present study suggests that the extracts analyzed could protect the liver cells from CCl4-induced liver damage by their antioxidative effect on hepatocytes.
PB  - Versita, Warsaw
T2  - Central European Journal of Biology
T1  - In vivo and in vitro antioxidant effects of three Veronica species
VL  - 7
IS  - 3
SP  - 559
EP  - 568
DO  - 10.2478/s11535-012-0041-4
ER  - 

@article{
author = "Živković, Jelena and Cebović, Tatjana and Maksimović, Zoran",
year = "2012",
abstract = "The aim of the present study was to examine the antioxidant activity of three Veronica species (Plantaginaceae). The antioxidant potential of various extracts obtained from aerial flowering parts was evaluated by DPPH-free (1,1-diphenyl-2-picrylhydrazyl-free) radical scavenging activity and ferric-reducing antioxidant power assays. Considerable antioxidant activity was observed in the plant samples (FRAP values ranged from 0.97 to 4.85 mmol Fe2+/g, and DPPH IC50 values from 12.58 to 66.34 A mu g/ml); however, these levels were lower than the activity of the control compound butylated hydroxytoluene (BHT) (FRAP: 10.58 mmol Fe2+/g; DPPH IC50: 9.57 A mu g/ml). Also, the in vivo antioxidant effects were evaluated in several hepatic antioxidant systems in rats (activities of glutathione peroxidase, glutathione reductase, peroxidase, catalase, xanthine oxidase, glutathione content and level of thiobarbituric acid reactive substances) after treatment with different Veronica extracts, or in combination with carbon tetrachloride (CCl4). Pretreatment with 100 mg/kg b.w. of Veronica extracts inhibited CCl4-induced liver injury by decreasing TBA-RS level, increasing GSH content, and bringing the activities of CAT and Px to control levels. The present study suggests that the extracts analyzed could protect the liver cells from CCl4-induced liver damage by their antioxidative effect on hepatocytes.",
publisher = "Versita, Warsaw",
journal = "Central European Journal of Biology",
title = "In vivo and in vitro antioxidant effects of three Veronica species",
volume = "7",
number = "3",
pages = "559-568",
doi = "10.2478/s11535-012-0041-4"
}

Živković, J., Cebović, T.,& Maksimović, Z.. (2012). In vivo and in vitro antioxidant effects of three Veronica species. in Central European Journal of Biology
Versita, Warsaw., 7(3), 559-568.
https://doi.org/10.2478/s11535-012-0041-4

Živković J, Cebović T, Maksimović Z. In vivo and in vitro antioxidant effects of three Veronica species. in Central European Journal of Biology. 2012;7(3):559-568.
doi:10.2478/s11535-012-0041-4 .

Živković, Jelena, Cebović, Tatjana, Maksimović, Zoran, "In vivo and in vitro antioxidant effects of three Veronica species" in Central European Journal of Biology, 7, no. 3 (2012):559-568,
https://doi.org/10.2478/s11535-012-0041-4 . .