TY  - JOUR
AU  - Grozdanić, Nađa
AU  - Đuričić, Ivana
AU  - Kosanić, Marijana
AU  - Zdunić, Gordana
AU  - Šavikin, Katarina
AU  - Etahiri, Samira
AU  - Assobhei, Omar
AU  - Benba, Jamila
AU  - Petović, Slavica
AU  - Matić, Ivana Z.
AU  - Stanojković, Tatjana P.
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3605
AB  - Purpose: Sea macroalgae are an important source of biologically highly valuable compounds. The main aim of this study was to investigate the in vitro anticancer properties and chemical composition of the dichloromethane-methanol extract and three fractions of the Fucus spiralis from coastline of Morocco. Methods: Fractions were made from dichloromethane: methanol (1:1) extract of Fucus spiralis: petroleum-ether, ethyl-acetate and n-butanol. Extract and fractions were screened for in vitro cytotoxicity by MTT assay against human cervical adenocarcinoma (HeLa), colorectal adenocarcinoma (LS-174T), lung carcinoma (A549), and normal human lung fibroblasts (MRC-5). Cell cycle distribution of the HeLa cells was evaluated using flow cytometry. Acridine orange (AO)-ethidium bromide (EB) staining was used to assess morphological changes of HeLa cells under fluorescence microscope. Anti-migration and anti-angiogenic properties were investigated using scratch and tube formation assays against human endothelium-derived permanent EA.hy926 cell line. Antidiabetic activity was tested using anti-α-glucosidase assay. Antimicrobial effect was tested using micro- dilution method. Results: Petroleum-ether fraction оf Fucus spiralis rich in fatty acids exerted the highest cytotoxicity against HeLa cells. Ethyl-acetate and petroleum-ether fractions induced the highest accumulation of the HeLa cells in sub-G1 and G2/M phases. Extract and fractions showed proapoptotic effect on HeLa cells under fluorescent microscope. They exhibited antimigratory and antiangiogenic effects in vitro. IC50 value for α-glucosidase inhibitory activity was much stronger than standard acarbose. n-Butanol fraction exerted the highest antibacterial and antifungal activity. Conclusions: The investigation of various biological activities of the extract and fractions obtained from Fucus spiralis may suggest a promising anticancer and pharmacological potential of this edible macroalga.
PB  - Zerbinis Publications
T2  - Journal of B.U.ON.
T1  - Fucus spiralis extract and fractions: Anticancer and pharmacological potentials
VL  - 25
IS  - 2
SP  - 1219
EP  - 1229
UR  - https://hdl.handle.net/21.15107/rcub_farfar_3605
ER  - 

@article{
author = "Grozdanić, Nađa and Đuričić, Ivana and Kosanić, Marijana and Zdunić, Gordana and Šavikin, Katarina and Etahiri, Samira and Assobhei, Omar and Benba, Jamila and Petović, Slavica and Matić, Ivana Z. and Stanojković, Tatjana P.",
year = "2020",
abstract = "Purpose: Sea macroalgae are an important source of biologically highly valuable compounds. The main aim of this study was to investigate the in vitro anticancer properties and chemical composition of the dichloromethane-methanol extract and three fractions of the Fucus spiralis from coastline of Morocco. Methods: Fractions were made from dichloromethane: methanol (1:1) extract of Fucus spiralis: petroleum-ether, ethyl-acetate and n-butanol. Extract and fractions were screened for in vitro cytotoxicity by MTT assay against human cervical adenocarcinoma (HeLa), colorectal adenocarcinoma (LS-174T), lung carcinoma (A549), and normal human lung fibroblasts (MRC-5). Cell cycle distribution of the HeLa cells was evaluated using flow cytometry. Acridine orange (AO)-ethidium bromide (EB) staining was used to assess morphological changes of HeLa cells under fluorescence microscope. Anti-migration and anti-angiogenic properties were investigated using scratch and tube formation assays against human endothelium-derived permanent EA.hy926 cell line. Antidiabetic activity was tested using anti-α-glucosidase assay. Antimicrobial effect was tested using micro- dilution method. Results: Petroleum-ether fraction оf Fucus spiralis rich in fatty acids exerted the highest cytotoxicity against HeLa cells. Ethyl-acetate and petroleum-ether fractions induced the highest accumulation of the HeLa cells in sub-G1 and G2/M phases. Extract and fractions showed proapoptotic effect on HeLa cells under fluorescent microscope. They exhibited antimigratory and antiangiogenic effects in vitro. IC50 value for α-glucosidase inhibitory activity was much stronger than standard acarbose. n-Butanol fraction exerted the highest antibacterial and antifungal activity. Conclusions: The investigation of various biological activities of the extract and fractions obtained from Fucus spiralis may suggest a promising anticancer and pharmacological potential of this edible macroalga.",
publisher = "Zerbinis Publications",
journal = "Journal of B.U.ON.",
title = "Fucus spiralis extract and fractions: Anticancer and pharmacological potentials",
volume = "25",
number = "2",
pages = "1219-1229",
url = "https://hdl.handle.net/21.15107/rcub_farfar_3605"
}

Grozdanić, N., Đuričić, I., Kosanić, M., Zdunić, G., Šavikin, K., Etahiri, S., Assobhei, O., Benba, J., Petović, S., Matić, I. Z.,& Stanojković, T. P.. (2020). Fucus spiralis extract and fractions: Anticancer and pharmacological potentials. in Journal of B.U.ON.
Zerbinis Publications., 25(2), 1219-1229.
https://hdl.handle.net/21.15107/rcub_farfar_3605

Grozdanić N, Đuričić I, Kosanić M, Zdunić G, Šavikin K, Etahiri S, Assobhei O, Benba J, Petović S, Matić IZ, Stanojković TP. Fucus spiralis extract and fractions: Anticancer and pharmacological potentials. in Journal of B.U.ON.. 2020;25(2):1219-1229.
https://hdl.handle.net/21.15107/rcub_farfar_3605 .

Grozdanić, Nađa, Đuričić, Ivana, Kosanić, Marijana, Zdunić, Gordana, Šavikin, Katarina, Etahiri, Samira, Assobhei, Omar, Benba, Jamila, Petović, Slavica, Matić, Ivana Z., Stanojković, Tatjana P., "Fucus spiralis extract and fractions: Anticancer and pharmacological potentials" in Journal of B.U.ON., 25, no. 2 (2020):1219-1229,
https://hdl.handle.net/21.15107/rcub_farfar_3605 .

TY  - JOUR
AU  - Marković, Violeta
AU  - Erić, Slavica
AU  - Juranić, Zorica
AU  - Stanojković, Tatjana
AU  - Joksović, Ljubinka
AU  - Ranković, Branislav
AU  - Kosanić, Marijana
AU  - Joksović, Milan D.
PY  - 2011
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1500
AB  - A series of aminomethylidene derivatives obtained from 4-formyledaravone were synthesized and characterized by IR, NMR and elemental analysis. All the compounds were screened for their antitumor activity. The compound containing 5-phenylpyrazole moiety (3q) exhibited remarkable antitumor activity in in vitro assays, especially against human breast cancer MDA-MB-361 and MDA-MB-453 cell lines. The most important whole-molecule descriptors for antitumor activity on MDA-MB-453 cells belong to the group of quantum-chemical descriptors.
PB  - Academic Press Inc Elsevier Science, San Diego
T2  - Bioorganic Chemistry
T1  - Synthesis, antitumor activity and QSAR studies of some 4-aminomethylidene derivatives of edaravone
VL  - 39
IS  - 1-3
SP  - 18
EP  - 27
DO  - 10.1016/j.bioorg.2010.10.003
ER  - 

@article{
author = "Marković, Violeta and Erić, Slavica and Juranić, Zorica and Stanojković, Tatjana and Joksović, Ljubinka and Ranković, Branislav and Kosanić, Marijana and Joksović, Milan D.",
year = "2011",
abstract = "A series of aminomethylidene derivatives obtained from 4-formyledaravone were synthesized and characterized by IR, NMR and elemental analysis. All the compounds were screened for their antitumor activity. The compound containing 5-phenylpyrazole moiety (3q) exhibited remarkable antitumor activity in in vitro assays, especially against human breast cancer MDA-MB-361 and MDA-MB-453 cell lines. The most important whole-molecule descriptors for antitumor activity on MDA-MB-453 cells belong to the group of quantum-chemical descriptors.",
publisher = "Academic Press Inc Elsevier Science, San Diego",
journal = "Bioorganic Chemistry",
title = "Synthesis, antitumor activity and QSAR studies of some 4-aminomethylidene derivatives of edaravone",
volume = "39",
number = "1-3",
pages = "18-27",
doi = "10.1016/j.bioorg.2010.10.003"
}

Marković, V., Erić, S., Juranić, Z., Stanojković, T., Joksović, L., Ranković, B., Kosanić, M.,& Joksović, M. D.. (2011). Synthesis, antitumor activity and QSAR studies of some 4-aminomethylidene derivatives of edaravone. in Bioorganic Chemistry
Academic Press Inc Elsevier Science, San Diego., 39(1-3), 18-27.
https://doi.org/10.1016/j.bioorg.2010.10.003

Marković V, Erić S, Juranić Z, Stanojković T, Joksović L, Ranković B, Kosanić M, Joksović MD. Synthesis, antitumor activity and QSAR studies of some 4-aminomethylidene derivatives of edaravone. in Bioorganic Chemistry. 2011;39(1-3):18-27.
doi:10.1016/j.bioorg.2010.10.003 .

Marković, Violeta, Erić, Slavica, Juranić, Zorica, Stanojković, Tatjana, Joksović, Ljubinka, Ranković, Branislav, Kosanić, Marijana, Joksović, Milan D., "Synthesis, antitumor activity and QSAR studies of some 4-aminomethylidene derivatives of edaravone" in Bioorganic Chemistry, 39, no. 1-3 (2011):18-27,
https://doi.org/10.1016/j.bioorg.2010.10.003 . .