TY  - CONF
AU  - Jakovljević, Dunja
AU  - Samardžić, Stevan
AU  - Majkić, Tatjana
AU  - Beara, Ivana
AU  - Maksimović, Zoran
AU  - Ćebović, Tatjana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4586
AB  - Curly dock (Rumex crispus L.) is a perennial herbaceous plant that is traditionally used
in the treatment of gastrointestinal ailments, infections and skin diseases. The aim of this
study was to examine the chemical composition, ex vivo anti-inflammatory effect and in vivo
antiproliferative activity of water extract of curly dock fruit. The polyphenolic profile was
analyzed by LC-DAD-ESI-MS technique. The effect on the production of arachidonic acid
metabolites was examined in human platelets. Antitumor activity of orally administered
extract (concentration: 5% w/v; doses: 1, 2 and 5 mL/kg of body weight/day; treatment
length: 7 days) to Ehrlich ascites tumor and hepatocellular carcinoma was assessed in an
animal experiment. Ascites volume, viable cell percentage and antioxidant enzyme levels
were determined. The presence of phenolic acids (gallic and protocatechuic acids),
proanthocyanidins (monomers, dimers and trimers), flavonoid glycosides and
anthraquinone emodin was revealed in the water extract of R. crispus fruit. The content of
the quantitatively dominant compound miquelianin was 2.4%. The investigated preparation
concentration-dependently inhibited the synthesis of 12(S)-hydroxy-(5Z,8E,10E)-
heptadecatrienoic acid (IC50 = 4.96 mg/mL) and 12(S)-hydroxy-(5Z,8Z,10E,14Z)-
eicosatetraenoic acid (IC 50 = 2.06 mg/mL). The antitumor effect in animals was dose-
dependent and manifested as suppression of tumor growth and induction of cell death of
malignantly transformed cells. Values of biochemical parameters suggested that oxidative
stress was induced in cancer cells, and it can be assumed that this mechanism was at least
partly responsible for the activity. Water extract of R. crispus fruit has significant
pharmacological potential that justifies further research.
AB  - Štavelj (Rumex crispus L.) je višegodišnja zeljasta biljka koja se tradicionalno koristi u
tretmanu gastrointestinalnih tegoba, infekcija i kožnih oboljenja. Cilj ove studije je bio da se
ispita hemijski sastav, ex vivo antiinflamatorni efekat i in vivo antiproliferativna aktivnost
vodenog ekstrakta ploda štavelja. Polifenolni profil je analiziran LC‐DAD‐ESI‐MS tehnikom.
Uticaj na produkciju metabolita arahidonske kiseline je ispitan u humanim trombocitima.
Antitumorska aktivnost oralno primenjenog ekstrakta (koncentracija: 5% m/V; doze: 1, 2 i 5
mL/kg telesne težine/dan; dužina tretmana: 7 dana) prema Ehrlich-ovom ascitnom tumoru i
hepatocelularnom karcinomu procenjena je u eksperimentu na životinjama. Određeni su
zapremina ascitesa, procenat vijabilnih ćelija i nivoi antioksidantnih enzima. U vodenom
ekstraktu ploda R. crispus je utvrđeno prisustvo fenolnih kiselina (galna i protokatehinska
kiselina), proantocijanidina (monomeri, dimeri i trimeri), flavonoidnih heterozida i
antrahinona emodina. Sadržaj kvantitativno dominantnog jedinjenja mikvelijanina je iznosio
2,4%. Ispitivani preparat je koncentraciono-zavisno inhibirao sintezu 12(S)-hidroksi-
(5Z,8E,10E)-heptadekatrienske kiseline (IC 50 = 4,96 mg/mL) i 12(S)-hidroksi-
(5Z,8Z,10E,14Z)-eikozatetraenske kiseline (IC50 = 2,06 mg/mL). Antitumorski efekat u
životinja je bio dozno-zavisan i ispoljio se kao suprimiranje rasta tumora i indukcija ćelijske
smrti maligno transformisanih ćelija. Vrednosti biohemijskih parametara su sugerisale da je
u ćelijama karcinoma došlo do indukovanja oksidativnog stresa, te se može pretpostaviti da
je ovaj mehanizam bio barem delom zaslužan za aktivnost. Vodeni ekstrakt ploda R. crispus
poseduje značajan farmakološki potencijal koji opravdava dalje istraživanje.
PB  - Savez farmaceutskih udruženja Srbije (SFUS)
C3  - Arhiv za farmaciju
T1  - Chemical composition, anti-inflammatory effect and antitumor activity of curly dock fruit (Rumex crispus L., Polygonaceae)
T1  - Hemijski sastav, antiinflamatorni efekat i antitumorska aktivnost ploda štavelja (Rumex crispus L., Polygonaceae)
VL  - 72
IS  - 4 suplement
SP  - S479
EP  - S480
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4586
ER  - 

@conference{
author = "Jakovljević, Dunja and Samardžić, Stevan and Majkić, Tatjana and Beara, Ivana and Maksimović, Zoran and Ćebović, Tatjana",
year = "2022",
abstract = "Curly dock (Rumex crispus L.) is a perennial herbaceous plant that is traditionally used
in the treatment of gastrointestinal ailments, infections and skin diseases. The aim of this
study was to examine the chemical composition, ex vivo anti-inflammatory effect and in vivo
antiproliferative activity of water extract of curly dock fruit. The polyphenolic profile was
analyzed by LC-DAD-ESI-MS technique. The effect on the production of arachidonic acid
metabolites was examined in human platelets. Antitumor activity of orally administered
extract (concentration: 5% w/v; doses: 1, 2 and 5 mL/kg of body weight/day; treatment
length: 7 days) to Ehrlich ascites tumor and hepatocellular carcinoma was assessed in an
animal experiment. Ascites volume, viable cell percentage and antioxidant enzyme levels
were determined. The presence of phenolic acids (gallic and protocatechuic acids),
proanthocyanidins (monomers, dimers and trimers), flavonoid glycosides and
anthraquinone emodin was revealed in the water extract of R. crispus fruit. The content of
the quantitatively dominant compound miquelianin was 2.4%. The investigated preparation
concentration-dependently inhibited the synthesis of 12(S)-hydroxy-(5Z,8E,10E)-
heptadecatrienoic acid (IC50 = 4.96 mg/mL) and 12(S)-hydroxy-(5Z,8Z,10E,14Z)-
eicosatetraenoic acid (IC 50 = 2.06 mg/mL). The antitumor effect in animals was dose-
dependent and manifested as suppression of tumor growth and induction of cell death of
malignantly transformed cells. Values of biochemical parameters suggested that oxidative
stress was induced in cancer cells, and it can be assumed that this mechanism was at least
partly responsible for the activity. Water extract of R. crispus fruit has significant
pharmacological potential that justifies further research., Štavelj (Rumex crispus L.) je višegodišnja zeljasta biljka koja se tradicionalno koristi u
tretmanu gastrointestinalnih tegoba, infekcija i kožnih oboljenja. Cilj ove studije je bio da se
ispita hemijski sastav, ex vivo antiinflamatorni efekat i in vivo antiproliferativna aktivnost
vodenog ekstrakta ploda štavelja. Polifenolni profil je analiziran LC‐DAD‐ESI‐MS tehnikom.
Uticaj na produkciju metabolita arahidonske kiseline je ispitan u humanim trombocitima.
Antitumorska aktivnost oralno primenjenog ekstrakta (koncentracija: 5% m/V; doze: 1, 2 i 5
mL/kg telesne težine/dan; dužina tretmana: 7 dana) prema Ehrlich-ovom ascitnom tumoru i
hepatocelularnom karcinomu procenjena je u eksperimentu na životinjama. Određeni su
zapremina ascitesa, procenat vijabilnih ćelija i nivoi antioksidantnih enzima. U vodenom
ekstraktu ploda R. crispus je utvrđeno prisustvo fenolnih kiselina (galna i protokatehinska
kiselina), proantocijanidina (monomeri, dimeri i trimeri), flavonoidnih heterozida i
antrahinona emodina. Sadržaj kvantitativno dominantnog jedinjenja mikvelijanina je iznosio
2,4%. Ispitivani preparat je koncentraciono-zavisno inhibirao sintezu 12(S)-hidroksi-
(5Z,8E,10E)-heptadekatrienske kiseline (IC 50 = 4,96 mg/mL) i 12(S)-hidroksi-
(5Z,8Z,10E,14Z)-eikozatetraenske kiseline (IC50 = 2,06 mg/mL). Antitumorski efekat u
životinja je bio dozno-zavisan i ispoljio se kao suprimiranje rasta tumora i indukcija ćelijske
smrti maligno transformisanih ćelija. Vrednosti biohemijskih parametara su sugerisale da je
u ćelijama karcinoma došlo do indukovanja oksidativnog stresa, te se može pretpostaviti da
je ovaj mehanizam bio barem delom zaslužan za aktivnost. Vodeni ekstrakt ploda R. crispus
poseduje značajan farmakološki potencijal koji opravdava dalje istraživanje.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "Chemical composition, anti-inflammatory effect and antitumor activity of curly dock fruit (Rumex crispus L., Polygonaceae), Hemijski sastav, antiinflamatorni efekat i antitumorska aktivnost ploda štavelja (Rumex crispus L., Polygonaceae)",
volume = "72",
number = "4 suplement",
pages = "S479-S480",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4586"
}

Jakovljević, D., Samardžić, S., Majkić, T., Beara, I., Maksimović, Z.,& Ćebović, T.. (2022). Chemical composition, anti-inflammatory effect and antitumor activity of curly dock fruit (Rumex crispus L., Polygonaceae). in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije (SFUS)., 72(4 suplement), S479-S480.
https://hdl.handle.net/21.15107/rcub_farfar_4586

Jakovljević D, Samardžić S, Majkić T, Beara I, Maksimović Z, Ćebović T. Chemical composition, anti-inflammatory effect and antitumor activity of curly dock fruit (Rumex crispus L., Polygonaceae). in Arhiv za farmaciju. 2022;72(4 suplement):S479-S480.
https://hdl.handle.net/21.15107/rcub_farfar_4586 .

Jakovljević, Dunja, Samardžić, Stevan, Majkić, Tatjana, Beara, Ivana, Maksimović, Zoran, Ćebović, Tatjana, "Chemical composition, anti-inflammatory effect and antitumor activity of curly dock fruit (Rumex crispus L., Polygonaceae)" in Arhiv za farmaciju, 72, no. 4 suplement (2022):S479-S480,
https://hdl.handle.net/21.15107/rcub_farfar_4586 .

TY  - JOUR
AU  - Beara, Ivana
AU  - Živković, Jelena
AU  - Lesjak, Marija
AU  - Ristić, Jelena
AU  - Šavikin, Katarina
AU  - Maksimović, Zoran
AU  - Janković, Teodora
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2377
AB  - Aerial parts of some Veronica species are used as traditional medicine for treatment of various inflammatory conditions, including rheumatism. In the present study, phenolic content and anti-inflammatory activity of three Veronica species (Plantaginaceae), Veronica jacquinii Baumg., Veronica teucrium L. and Veronica urticifolia Jacq was studied. The identification and quantification of 30 phenolic compounds in aqueous-acetone extracts were performed using LC-MS/MS technique, and the main components were baicalin, hyperoside, isoquercetine, chlorogenic acid and quinic acid. Genistein and baicalein were detected for the first time in Plantaginaceae family and Veronica genus, respectively. The anti-inflammatory potential of tested extracts was determined using calcium ionophore-stimulated human platelets as a source of cyclooxygenase-1 (COX-1) and 12-lipoxygenase (12-LOX) enzymes, and highly sensitive and specific LC-MS/MS technique was performed for detection of the main arachidonic acid metabolites formed by them. V. urticifolia extract exerted the strongest COX-1 inhibition (IC50  lt  0.4 mg/mL), while V. jacquinii extract exhibited the highest ability to inhibit 12-LOX activity (IC50 = 1.072 mg/mL). The results obtained in this study may be used to promote better use of the genus Veronica as anti-inflammatory agents.
PB  - Elsevier Science BV, Amsterdam
T2  - Industrial Crops and Products
T1  - Phenolic profile and anti-inflammatory activity of three Veronica species
VL  - 63
SP  - 276
EP  - 280
DO  - 10.1016/j.indcrop.2014.09.034
ER  - 

@article{
author = "Beara, Ivana and Živković, Jelena and Lesjak, Marija and Ristić, Jelena and Šavikin, Katarina and Maksimović, Zoran and Janković, Teodora",
year = "2015",
abstract = "Aerial parts of some Veronica species are used as traditional medicine for treatment of various inflammatory conditions, including rheumatism. In the present study, phenolic content and anti-inflammatory activity of three Veronica species (Plantaginaceae), Veronica jacquinii Baumg., Veronica teucrium L. and Veronica urticifolia Jacq was studied. The identification and quantification of 30 phenolic compounds in aqueous-acetone extracts were performed using LC-MS/MS technique, and the main components were baicalin, hyperoside, isoquercetine, chlorogenic acid and quinic acid. Genistein and baicalein were detected for the first time in Plantaginaceae family and Veronica genus, respectively. The anti-inflammatory potential of tested extracts was determined using calcium ionophore-stimulated human platelets as a source of cyclooxygenase-1 (COX-1) and 12-lipoxygenase (12-LOX) enzymes, and highly sensitive and specific LC-MS/MS technique was performed for detection of the main arachidonic acid metabolites formed by them. V. urticifolia extract exerted the strongest COX-1 inhibition (IC50  lt  0.4 mg/mL), while V. jacquinii extract exhibited the highest ability to inhibit 12-LOX activity (IC50 = 1.072 mg/mL). The results obtained in this study may be used to promote better use of the genus Veronica as anti-inflammatory agents.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Industrial Crops and Products",
title = "Phenolic profile and anti-inflammatory activity of three Veronica species",
volume = "63",
pages = "276-280",
doi = "10.1016/j.indcrop.2014.09.034"
}

Beara, I., Živković, J., Lesjak, M., Ristić, J., Šavikin, K., Maksimović, Z.,& Janković, T.. (2015). Phenolic profile and anti-inflammatory activity of three Veronica species. in Industrial Crops and Products
Elsevier Science BV, Amsterdam., 63, 276-280.
https://doi.org/10.1016/j.indcrop.2014.09.034

Beara I, Živković J, Lesjak M, Ristić J, Šavikin K, Maksimović Z, Janković T. Phenolic profile and anti-inflammatory activity of three Veronica species. in Industrial Crops and Products. 2015;63:276-280.
doi:10.1016/j.indcrop.2014.09.034 .

Beara, Ivana, Živković, Jelena, Lesjak, Marija, Ristić, Jelena, Šavikin, Katarina, Maksimović, Zoran, Janković, Teodora, "Phenolic profile and anti-inflammatory activity of three Veronica species" in Industrial Crops and Products, 63 (2015):276-280,
https://doi.org/10.1016/j.indcrop.2014.09.034 . .