TY  - JOUR
AU  - Pavlović, Miloš
AU  - Đukić, Mirjana
AU  - Vojvodić, Danilo
AU  - Ninković, Milica
AU  - Stevanović, Ivana
AU  - Đurić, Ana
AU  - Stanojević, Boban
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3956
AB  - Background/Aim. Exposure  of  living  organisms  to  γ- radiation results in the overproduction of free radicals. The  aim of the study was to test if the subacute administration  of micronized zeolite (MZC) accomplishes radioprotective  role based on the evaluation of the status of oxidative stress  (OS) in the brain and 8-hydroxyguanosine (8-OH-dG)  in  the plasma of the rats exposed to the single γ-ray irradiation  of 2 and/or 10 Gray (Gy). Methods. Wistar rats were on a  four-week normal or 5% MZC supplemented diet and af- terward exposed to the single γ-ray irradiation  of 2 and 10  Gy. Groups of rats were: a) on a normal diet (the control  group, and 2Gy and 10Gy groups); b) on 5% MZC supple- mented diet (the control group –  MZC, MZC  +  2Gy, and  MZC + 10Gy  groups).  We  measured  malondialdehyde  (MDA), glutathione (GSH) total, and activity of total super- oxide  dismutase  (tSOD)  and  manganese  superoxide  dis- mutase (MnSOD)  in vulnerable brain regions (cerebellum,  hippocampus, and cerebral cortex) and 8-OH-dG in plasma.  Results.  Lower MDA was found in the MZC+2Gy and  MZC+10Gy  groups  compared  to  the  2Gy  and  10Gy  groups.  Activity  od  total  SOD  was  higher  in  the  MZC+10Gy group  than in the 10Gy  one. GSH was the  highest in the 10Gy group. Compared to the control group,  8-OH-dG was extremely higher in groups radiated with 10  Gy regardless of a diet, but slightly lower in the MZC+2Gy  and 2Gy groups. Conclusion. Subacute MZC pretreatment  accomplished partial radioprotective effect in irradiated rats  compared to non-irradiated rats, based on suppressed SOD  activity at 2 Gy, and reduced brain MDA when exposed to 2  Gy and 10 Gy.
AB  - Uvod/Cilj.  Izlaganje živih organizama gama zračenju re- zultira hiperprodukcijom slobodnih radikala. Cilj istraživanja  je bio da se ispita da li subakutna ishrana  dopunjena  sa 5%  mikronizovanog zeolita (MZC) ispoljava radiozaštitnu ulogu  na osnovu statusa oksidativnog stresa (OS) u mozgu i 8- hidroksiguanozina  (8-OH-dG)  u  plazmi  pacova  izloženih  pojedinačnim dozama jonizujućeg zračenja od 2 i 10 Gray  (Gy).  Metode.  Wistar  pacovi  su  bili  na  četvoronedeljnoj  normalnoj ishrani ili ishrani obogaćenoj sa 5% MZC, posle  čega su bili izloženi pojedinačnom jonizujućem zračenju od  2  Gy,  odnosno  10  Gy.  Grupe  pacova  bile  su:  a)  gru pa  pacova na normalnoj ishrani (kontrolna grupa i grupe 2Gy i  10Gy); b) grupa pacova na ishrani obogaćenoj sa 5%  MZC  (kontrolna  grupa  – MZC  i  grupe  MZC+2Gy  i   MZC+10Gy).  Meren  je  malondialdehid  (MDA),  glutation  (GSH)  i  aktivnost  ukupne  (tSOD)  i  mangan  superoksid  dizmutaze  (MnSOD)  u  osetljivim  strukturama  mozga  (cerebelum, hipokampus i cerebralni korteks), a 8-OH-dG u  plazmi.  Rezultati. Biomarker  MDA  je  bio  niži  u  MZC+2Gy i MZC+10Gy grupama, u odnosu na grupe 2Gy  i  10Gy.  Aktivnost  ukupne SOD  je  bila  viša  u  grupi  MZC+10Gy, u odnosu na grupu 10Gy. Najviši nivo GSH  bio je u grupi 10Gy. U pređenju sa kontrolnom grupom, 8- OH-dG je bio izuzetno viši u grupama ozračenim sa 10 Gy,  bez obzira na dijetetski režim i niži u grupama MZC+2Gy i  2Gy.  Zaključak. Pacovi  koji  su  bili  na  režimu  ishrane  obogaćene  sa  5%  MZC  bili  su  delimično  zaštićeni  od  zračenja, shodno redukovanoj moždanoj aktivnosti SOD pri  2  Gy i sniženom nivou MDA pri izlaganju zračenju od 2 i  10 Gy.
PB  - Belgrade: Military Medical Academy, INI
T2  - Vojnosanitetski pregled
T1  - Moderate radioprotective role of zeolite in rats
T1  - Umerena radioprotektivna uloga zeolita kod pacova
VL  - 78
IS  - 7
SP  - 760
EP  - 768
DO  - 10.2298/VSP190702136P
ER  - 

@article{
author = "Pavlović, Miloš and Đukić, Mirjana and Vojvodić, Danilo and Ninković, Milica and Stevanović, Ivana and Đurić, Ana and Stanojević, Boban",
year = "2021",
abstract = "Background/Aim. Exposure  of  living  organisms  to  γ- radiation results in the overproduction of free radicals. The  aim of the study was to test if the subacute administration  of micronized zeolite (MZC) accomplishes radioprotective  role based on the evaluation of the status of oxidative stress  (OS) in the brain and 8-hydroxyguanosine (8-OH-dG)  in  the plasma of the rats exposed to the single γ-ray irradiation  of 2 and/or 10 Gray (Gy). Methods. Wistar rats were on a  four-week normal or 5% MZC supplemented diet and af- terward exposed to the single γ-ray irradiation  of 2 and 10  Gy. Groups of rats were: a) on a normal diet (the control  group, and 2Gy and 10Gy groups); b) on 5% MZC supple- mented diet (the control group –  MZC, MZC  +  2Gy, and  MZC + 10Gy  groups).  We  measured  malondialdehyde  (MDA), glutathione (GSH) total, and activity of total super- oxide  dismutase  (tSOD)  and  manganese  superoxide  dis- mutase (MnSOD)  in vulnerable brain regions (cerebellum,  hippocampus, and cerebral cortex) and 8-OH-dG in plasma.  Results.  Lower MDA was found in the MZC+2Gy and  MZC+10Gy  groups  compared  to  the  2Gy  and  10Gy  groups.  Activity  od  total  SOD  was  higher  in  the  MZC+10Gy group  than in the 10Gy  one. GSH was the  highest in the 10Gy group. Compared to the control group,  8-OH-dG was extremely higher in groups radiated with 10  Gy regardless of a diet, but slightly lower in the MZC+2Gy  and 2Gy groups. Conclusion. Subacute MZC pretreatment  accomplished partial radioprotective effect in irradiated rats  compared to non-irradiated rats, based on suppressed SOD  activity at 2 Gy, and reduced brain MDA when exposed to 2  Gy and 10 Gy., Uvod/Cilj.  Izlaganje živih organizama gama zračenju re- zultira hiperprodukcijom slobodnih radikala. Cilj istraživanja  je bio da se ispita da li subakutna ishrana  dopunjena  sa 5%  mikronizovanog zeolita (MZC) ispoljava radiozaštitnu ulogu  na osnovu statusa oksidativnog stresa (OS) u mozgu i 8- hidroksiguanozina  (8-OH-dG)  u  plazmi  pacova  izloženih  pojedinačnim dozama jonizujućeg zračenja od 2 i 10 Gray  (Gy).  Metode.  Wistar  pacovi  su  bili  na  četvoronedeljnoj  normalnoj ishrani ili ishrani obogaćenoj sa 5% MZC, posle  čega su bili izloženi pojedinačnom jonizujućem zračenju od  2  Gy,  odnosno  10  Gy.  Grupe  pacova  bile  su:  a)  gru pa  pacova na normalnoj ishrani (kontrolna grupa i grupe 2Gy i  10Gy); b) grupa pacova na ishrani obogaćenoj sa 5%  MZC  (kontrolna  grupa  – MZC  i  grupe  MZC+2Gy  i   MZC+10Gy).  Meren  je  malondialdehid  (MDA),  glutation  (GSH)  i  aktivnost  ukupne  (tSOD)  i  mangan  superoksid  dizmutaze  (MnSOD)  u  osetljivim  strukturama  mozga  (cerebelum, hipokampus i cerebralni korteks), a 8-OH-dG u  plazmi.  Rezultati. Biomarker  MDA  je  bio  niži  u  MZC+2Gy i MZC+10Gy grupama, u odnosu na grupe 2Gy  i  10Gy.  Aktivnost  ukupne SOD  je  bila  viša  u  grupi  MZC+10Gy, u odnosu na grupu 10Gy. Najviši nivo GSH  bio je u grupi 10Gy. U pređenju sa kontrolnom grupom, 8- OH-dG je bio izuzetno viši u grupama ozračenim sa 10 Gy,  bez obzira na dijetetski režim i niži u grupama MZC+2Gy i  2Gy.  Zaključak. Pacovi  koji  su  bili  na  režimu  ishrane  obogaćene  sa  5%  MZC  bili  su  delimično  zaštićeni  od  zračenja, shodno redukovanoj moždanoj aktivnosti SOD pri  2  Gy i sniženom nivou MDA pri izlaganju zračenju od 2 i  10 Gy.",
publisher = "Belgrade: Military Medical Academy, INI",
journal = "Vojnosanitetski pregled",
title = "Moderate radioprotective role of zeolite in rats, Umerena radioprotektivna uloga zeolita kod pacova",
volume = "78",
number = "7",
pages = "760-768",
doi = "10.2298/VSP190702136P"
}

Pavlović, M., Đukić, M., Vojvodić, D., Ninković, M., Stevanović, I., Đurić, A.,& Stanojević, B.. (2021). Moderate radioprotective role of zeolite in rats. in Vojnosanitetski pregled
Belgrade: Military Medical Academy, INI., 78(7), 760-768.
https://doi.org/10.2298/VSP190702136P

Pavlović M, Đukić M, Vojvodić D, Ninković M, Stevanović I, Đurić A, Stanojević B. Moderate radioprotective role of zeolite in rats. in Vojnosanitetski pregled. 2021;78(7):760-768.
doi:10.2298/VSP190702136P .

Pavlović, Miloš, Đukić, Mirjana, Vojvodić, Danilo, Ninković, Milica, Stevanović, Ivana, Đurić, Ana, Stanojević, Boban, "Moderate radioprotective role of zeolite in rats" in Vojnosanitetski pregled, 78, no. 7 (2021):760-768,
https://doi.org/10.2298/VSP190702136P . .

TY  - JOUR
AU  - Stevanović, Ivana
AU  - Ninković, Milica
AU  - Mančić, Bojana
AU  - Milivojević, Marija
AU  - Stojanović, Ivana
AU  - Ilić, Tihomir
AU  - Vujović, Maja
AU  - Đukić, Mirjana
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3658
AB  - Cortical theta burst stimulation (TBS) structured as intermittent (iTBS) and continuous (cTBS)could prevent the progression of the experimental autoimmune encephalomyelitis (EAE). The interplayof brain antioxidant defense systems against free radicals (FRs) overproduction induced by EAE,as well as during iTBS or cTBS, have not been entirely investigated.  This study aimed to examinewhether oxidative-nitrogen stress (ONS) is one of the underlying pathophysiological mechanisms ofEAE, which may be changed in terms of health improvement by iTBS or cTBS. Dark Agouti strainfemale rats were tested for the effects of EAE and TBS. The rats were randomly divided into the controlgroup, rats specifically immunized for EAE and nonspecifically immuno-stimulated with CompleteFreund’s adjuvant. TBS or sham TBS was applied to EAE rats from 14th–24th post-immunizationday. Superoxide dismutase activity, levels of superoxide anion (O2•–), lipid peroxidation, glutathione(GSH), nicotinamide adenine dinucleotide phosphate (NADPH), and thioredoxin reductase (TrxR)activity were analyzed in rat spinal cords homogenates. The severity of EAE clinical coincided withthe climax of ONS. The most critical result refers to TrxR, which immensely responded against theapplied stressors of the central nervous system (CNS), including immunization and TBS. We foundthat the compensatory neuroprotective role of TrxR upregulation is a positive feedback mechanismthat reduces the harmfulness of ONS. iTBS and cTBS both modulate the biochemical environmentagainst ONS at a distance from the area of stimulation, alleviating symptoms of EAE. The results ofour study increase the understanding of FRs’ interplay and the role of Trx/TrxR in ONS-associatedneuroinflammatory diseases, such as EAE. Also, our results might help the development of new ideasfor designing more effective medical treatment, combining neuropsychological with noninvasiveneurostimulation–neuromodulation techniques to patients living with MS.
PB  - MDPI
T2  - Molecules
T1  - Compensatory Neuroprotective Response of Thioredoxin Reductase against Oxidative-Nitrosative Stress Induced by Experimental Autoimmune Encephalomyelitis in Rats: Modulation by Theta Burst Stimulation
VL  - 25
IS  - 17
DO  - 10.3390/molecules25173922
ER  - 

@article{
author = "Stevanović, Ivana and Ninković, Milica and Mančić, Bojana and Milivojević, Marija and Stojanović, Ivana and Ilić, Tihomir and Vujović, Maja and Đukić, Mirjana",
year = "2020",
abstract = "Cortical theta burst stimulation (TBS) structured as intermittent (iTBS) and continuous (cTBS)could prevent the progression of the experimental autoimmune encephalomyelitis (EAE). The interplayof brain antioxidant defense systems against free radicals (FRs) overproduction induced by EAE,as well as during iTBS or cTBS, have not been entirely investigated.  This study aimed to examinewhether oxidative-nitrogen stress (ONS) is one of the underlying pathophysiological mechanisms ofEAE, which may be changed in terms of health improvement by iTBS or cTBS. Dark Agouti strainfemale rats were tested for the effects of EAE and TBS. The rats were randomly divided into the controlgroup, rats specifically immunized for EAE and nonspecifically immuno-stimulated with CompleteFreund’s adjuvant. TBS or sham TBS was applied to EAE rats from 14th–24th post-immunizationday. Superoxide dismutase activity, levels of superoxide anion (O2•–), lipid peroxidation, glutathione(GSH), nicotinamide adenine dinucleotide phosphate (NADPH), and thioredoxin reductase (TrxR)activity were analyzed in rat spinal cords homogenates. The severity of EAE clinical coincided withthe climax of ONS. The most critical result refers to TrxR, which immensely responded against theapplied stressors of the central nervous system (CNS), including immunization and TBS. We foundthat the compensatory neuroprotective role of TrxR upregulation is a positive feedback mechanismthat reduces the harmfulness of ONS. iTBS and cTBS both modulate the biochemical environmentagainst ONS at a distance from the area of stimulation, alleviating symptoms of EAE. The results ofour study increase the understanding of FRs’ interplay and the role of Trx/TrxR in ONS-associatedneuroinflammatory diseases, such as EAE. Also, our results might help the development of new ideasfor designing more effective medical treatment, combining neuropsychological with noninvasiveneurostimulation–neuromodulation techniques to patients living with MS.",
publisher = "MDPI",
journal = "Molecules",
title = "Compensatory Neuroprotective Response of Thioredoxin Reductase against Oxidative-Nitrosative Stress Induced by Experimental Autoimmune Encephalomyelitis in Rats: Modulation by Theta Burst Stimulation",
volume = "25",
number = "17",
doi = "10.3390/molecules25173922"
}

Stevanović, I., Ninković, M., Mančić, B., Milivojević, M., Stojanović, I., Ilić, T., Vujović, M.,& Đukić, M.. (2020). Compensatory Neuroprotective Response of Thioredoxin Reductase against Oxidative-Nitrosative Stress Induced by Experimental Autoimmune Encephalomyelitis in Rats: Modulation by Theta Burst Stimulation. in Molecules
MDPI., 25(17).
https://doi.org/10.3390/molecules25173922

Stevanović I, Ninković M, Mančić B, Milivojević M, Stojanović I, Ilić T, Vujović M, Đukić M. Compensatory Neuroprotective Response of Thioredoxin Reductase against Oxidative-Nitrosative Stress Induced by Experimental Autoimmune Encephalomyelitis in Rats: Modulation by Theta Burst Stimulation. in Molecules. 2020;25(17).
doi:10.3390/molecules25173922 .

Stevanović, Ivana, Ninković, Milica, Mančić, Bojana, Milivojević, Marija, Stojanović, Ivana, Ilić, Tihomir, Vujović, Maja, Đukić, Mirjana, "Compensatory Neuroprotective Response of Thioredoxin Reductase against Oxidative-Nitrosative Stress Induced by Experimental Autoimmune Encephalomyelitis in Rats: Modulation by Theta Burst Stimulation" in Molecules, 25, no. 17 (2020),
https://doi.org/10.3390/molecules25173922 . .

TY  - JOUR
AU  - Dejanović, Bratislav
AU  - Vuković-Dejanović, Vesna
AU  - Ninković, Milica
AU  - Lavrnja, Irena
AU  - Stojanović, Ivana
AU  - Pavlović, Miloš
AU  - Begović, Vesna
AU  - Mirković, Duško
AU  - Stevanović, Ivana
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3222
AB  - This study was aimed to study the potentially beneficial effects of agmatine on oxidative/nitrosative stress development in the brain of Wistar rats during subacute chlorpromazine treatment. The animals were divided into control (0.9% saline), chlorpromazine (38.7 mg/kg b.w.), chlorpromazine+agmatine (agmatine 75 mg/kg b.w. immediately after chlorpromazine, 38.7 mg/ kg b.w. i.p.) and agmatine (75 mg/kg b.w.) groups. All the tested substances were administered intraperitoneally for 15 consecutive days and the rats were sacrificed by decapitation on day 15. Subacute administration of chlorpromazine resulted in increased lipid peroxidation, nitric oxide concentration and superoxide anion production, while completely damaging the antioxidant defence system in the cerebral cortex, striatum, and hippocampus. However, the combined treatment with chlorpromazine and agmatine significantly attenuated the oxidative/nitrosative stress indices and restored the antioxidant capacity to the control values in all of the examined brain regions. Western blot analysis supported biochemical findings in all groups, but the most notable changes were found in the hippocampus. Our results suggest potentially beneficial effects of agmatine, which may be useful in the modified antioxidant approach in chlorpromazine-therapy.
PB  - Veterinarni A Farmaceuticka Univerzita Brno, Brno
T2  - Acta Veterinaria Brno
T1  - Effects of agmatine on chlorpromazine-induced neuronal injury in rat
VL  - 87
IS  - 2
SP  - 145
EP  - 153
DO  - 10.2754/avb201887020145
ER  - 

@article{
author = "Dejanović, Bratislav and Vuković-Dejanović, Vesna and Ninković, Milica and Lavrnja, Irena and Stojanović, Ivana and Pavlović, Miloš and Begović, Vesna and Mirković, Duško and Stevanović, Ivana",
year = "2018",
abstract = "This study was aimed to study the potentially beneficial effects of agmatine on oxidative/nitrosative stress development in the brain of Wistar rats during subacute chlorpromazine treatment. The animals were divided into control (0.9% saline), chlorpromazine (38.7 mg/kg b.w.), chlorpromazine+agmatine (agmatine 75 mg/kg b.w. immediately after chlorpromazine, 38.7 mg/ kg b.w. i.p.) and agmatine (75 mg/kg b.w.) groups. All the tested substances were administered intraperitoneally for 15 consecutive days and the rats were sacrificed by decapitation on day 15. Subacute administration of chlorpromazine resulted in increased lipid peroxidation, nitric oxide concentration and superoxide anion production, while completely damaging the antioxidant defence system in the cerebral cortex, striatum, and hippocampus. However, the combined treatment with chlorpromazine and agmatine significantly attenuated the oxidative/nitrosative stress indices and restored the antioxidant capacity to the control values in all of the examined brain regions. Western blot analysis supported biochemical findings in all groups, but the most notable changes were found in the hippocampus. Our results suggest potentially beneficial effects of agmatine, which may be useful in the modified antioxidant approach in chlorpromazine-therapy.",
publisher = "Veterinarni A Farmaceuticka Univerzita Brno, Brno",
journal = "Acta Veterinaria Brno",
title = "Effects of agmatine on chlorpromazine-induced neuronal injury in rat",
volume = "87",
number = "2",
pages = "145-153",
doi = "10.2754/avb201887020145"
}

Dejanović, B., Vuković-Dejanović, V., Ninković, M., Lavrnja, I., Stojanović, I., Pavlović, M., Begović, V., Mirković, D.,& Stevanović, I.. (2018). Effects of agmatine on chlorpromazine-induced neuronal injury in rat. in Acta Veterinaria Brno
Veterinarni A Farmaceuticka Univerzita Brno, Brno., 87(2), 145-153.
https://doi.org/10.2754/avb201887020145

Dejanović B, Vuković-Dejanović V, Ninković M, Lavrnja I, Stojanović I, Pavlović M, Begović V, Mirković D, Stevanović I. Effects of agmatine on chlorpromazine-induced neuronal injury in rat. in Acta Veterinaria Brno. 2018;87(2):145-153.
doi:10.2754/avb201887020145 .

Dejanović, Bratislav, Vuković-Dejanović, Vesna, Ninković, Milica, Lavrnja, Irena, Stojanović, Ivana, Pavlović, Miloš, Begović, Vesna, Mirković, Duško, Stevanović, Ivana, "Effects of agmatine on chlorpromazine-induced neuronal injury in rat" in Acta Veterinaria Brno, 87, no. 2 (2018):145-153,
https://doi.org/10.2754/avb201887020145 . .

TY  - JOUR
AU  - Taso, Ervin
AU  - Stefanović, Vladimir
AU  - Stevanović, Ivana
AU  - Vojvodić, Danilo
AU  - Topić, Aleksandra
AU  - Petković-Ćurčin, Aleksandra
AU  - Obradović-Đuričić, Kosovka
AU  - Marković, Aleksa
AU  - Đukić, Mirjana
AU  - Vujanović, Dragana
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3219
AB  - Biocompatibility of dental materials (DM) can be evaluated by gingival crevicular fluid (GCF) oxidative stress (OS) status. The goal of the study was to ascertain influence of dental caries degree, teeth position, and type and amount of applied DM on GCF OS profile. For this purpose, we tested six DMs that were sealed in one session: amalgam (Amg), composites: Tetric EvoCeram and Beautifil (BF), phosphate cement-zinc phosphate and polycarboxylate cements zinc polycarboxylate cements, and glass ionomer cement (GIC). The study included 88 dental outpatients. Follow-up was scheduled at 7th and 30th day. Oxidative stress parameters (malondialdehyde (MDA) and glutathione (GSH) levels and total superoxide dismutase (tSOD) activity) were measured before (0th day) and after the treatment (7th and 30th day) in GCF. Control teeth were mirror-positioned healthy teeth. The DM accomplished the following effects (listed in descending order): increase of GSH in GCF was realized by ZPoC > BF > GIC > Amg; tSOD activity increase by ZPoC > BF > Amg; and MDA decrease by ZPoC > ZPhC > Amg > TEC. Dental caries provokes insignificant rise of OS in GCF. ZPoC and ZPhC showed the highest antioxidant effect, contrary to GIC. Restorations with antioxidant properties may reduce gum diseases initiated by caries lesion, what is of great clinical relevance in dentistry.
PB  - Hindawi Ltd, London
T2  - Oxidative Medicine and Cellular Longevity
T1  - Influence of Dental Restorations on Oxidative Stress in Gingival Crevicular Fluid
DO  - 10.1155/2018/1823189
ER  - 

@article{
author = "Taso, Ervin and Stefanović, Vladimir and Stevanović, Ivana and Vojvodić, Danilo and Topić, Aleksandra and Petković-Ćurčin, Aleksandra and Obradović-Đuričić, Kosovka and Marković, Aleksa and Đukić, Mirjana and Vujanović, Dragana",
year = "2018",
abstract = "Biocompatibility of dental materials (DM) can be evaluated by gingival crevicular fluid (GCF) oxidative stress (OS) status. The goal of the study was to ascertain influence of dental caries degree, teeth position, and type and amount of applied DM on GCF OS profile. For this purpose, we tested six DMs that were sealed in one session: amalgam (Amg), composites: Tetric EvoCeram and Beautifil (BF), phosphate cement-zinc phosphate and polycarboxylate cements zinc polycarboxylate cements, and glass ionomer cement (GIC). The study included 88 dental outpatients. Follow-up was scheduled at 7th and 30th day. Oxidative stress parameters (malondialdehyde (MDA) and glutathione (GSH) levels and total superoxide dismutase (tSOD) activity) were measured before (0th day) and after the treatment (7th and 30th day) in GCF. Control teeth were mirror-positioned healthy teeth. The DM accomplished the following effects (listed in descending order): increase of GSH in GCF was realized by ZPoC > BF > GIC > Amg; tSOD activity increase by ZPoC > BF > Amg; and MDA decrease by ZPoC > ZPhC > Amg > TEC. Dental caries provokes insignificant rise of OS in GCF. ZPoC and ZPhC showed the highest antioxidant effect, contrary to GIC. Restorations with antioxidant properties may reduce gum diseases initiated by caries lesion, what is of great clinical relevance in dentistry.",
publisher = "Hindawi Ltd, London",
journal = "Oxidative Medicine and Cellular Longevity",
title = "Influence of Dental Restorations on Oxidative Stress in Gingival Crevicular Fluid",
doi = "10.1155/2018/1823189"
}

Taso, E., Stefanović, V., Stevanović, I., Vojvodić, D., Topić, A., Petković-Ćurčin, A., Obradović-Đuričić, K., Marković, A., Đukić, M.,& Vujanović, D.. (2018). Influence of Dental Restorations on Oxidative Stress in Gingival Crevicular Fluid. in Oxidative Medicine and Cellular Longevity
Hindawi Ltd, London..
https://doi.org/10.1155/2018/1823189

Taso E, Stefanović V, Stevanović I, Vojvodić D, Topić A, Petković-Ćurčin A, Obradović-Đuričić K, Marković A, Đukić M, Vujanović D. Influence of Dental Restorations on Oxidative Stress in Gingival Crevicular Fluid. in Oxidative Medicine and Cellular Longevity. 2018;.
doi:10.1155/2018/1823189 .

Taso, Ervin, Stefanović, Vladimir, Stevanović, Ivana, Vojvodić, Danilo, Topić, Aleksandra, Petković-Ćurčin, Aleksandra, Obradović-Đuričić, Kosovka, Marković, Aleksa, Đukić, Mirjana, Vujanović, Dragana, "Influence of Dental Restorations on Oxidative Stress in Gingival Crevicular Fluid" in Oxidative Medicine and Cellular Longevity (2018),
https://doi.org/10.1155/2018/1823189 . .

TY  - JOUR
AU  - Pantić Biševac, Jelena
AU  - Đukić, Mirjana
AU  - Stanojević, Ivan
AU  - Stevanović, Ivana
AU  - Mijušković, Željko
AU  - Đurić, Ana
AU  - Gobeljić, Borko
AU  - Banović, Tatjana
AU  - Vojvodić, Danilo
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3206
AB  - Background: Overproduction of free radicals accompanied with their insufficient removal/neutralization by antioxidative defense system impairs redox hemostasis in living organisms. Oxidative stress has been shown to be involved in all the stages of carcinogenesis and malignant melanocyte transformation. The aim of this study was to examine association between oxidative stress development and different stages of melanoma. Methods: The measured oxidative stress parameters included: superoxide anion radical, total and manganese superoxide dismutase, catalase and malondialdehyde. Oxidative stress parameters were measured spectrophotometrically in serum samples from melanoma patients (n=72) and healthy control subjects (n=30). Patients were classified according to AJCC clinical stage. Results: Average superoxide anion and malondialdehyde concentrations were significantly higher in melanoma patients than in control group, with the highest value of superoxide anion in stage III, while malondialdehyde highest value was in stage IV. The activity of total and manganese superoxide dismutase was insignificantly higher in melanoma patients than in control group, while catalase activity was significantly higher. The highest activity of total superoxide dismutase was in stage III, while the highest activity of manganese superoxide dismutase was in stage IV. Catalase activity was increasing with the disease progression achieving the maximum in stage III. Conclusions: Results of our study suggest that melanoma is oxidative stress associated disease, as well as deteriorated cell functioning at mitochondrial level.
PB  - Društvo medicinskih biohemičara Srbije, Beograd i Versita
T2  - Journal of Medical Biochemistry
T1  - Association between oxidative stress and melanoma progression
VL  - 37
IS  - 1
SP  - 12
EP  - 20
DO  - 10.1515/jomb-2017-0040
ER  - 

@article{
author = "Pantić Biševac, Jelena and Đukić, Mirjana and Stanojević, Ivan and Stevanović, Ivana and Mijušković, Željko and Đurić, Ana and Gobeljić, Borko and Banović, Tatjana and Vojvodić, Danilo",
year = "2018",
abstract = "Background: Overproduction of free radicals accompanied with their insufficient removal/neutralization by antioxidative defense system impairs redox hemostasis in living organisms. Oxidative stress has been shown to be involved in all the stages of carcinogenesis and malignant melanocyte transformation. The aim of this study was to examine association between oxidative stress development and different stages of melanoma. Methods: The measured oxidative stress parameters included: superoxide anion radical, total and manganese superoxide dismutase, catalase and malondialdehyde. Oxidative stress parameters were measured spectrophotometrically in serum samples from melanoma patients (n=72) and healthy control subjects (n=30). Patients were classified according to AJCC clinical stage. Results: Average superoxide anion and malondialdehyde concentrations were significantly higher in melanoma patients than in control group, with the highest value of superoxide anion in stage III, while malondialdehyde highest value was in stage IV. The activity of total and manganese superoxide dismutase was insignificantly higher in melanoma patients than in control group, while catalase activity was significantly higher. The highest activity of total superoxide dismutase was in stage III, while the highest activity of manganese superoxide dismutase was in stage IV. Catalase activity was increasing with the disease progression achieving the maximum in stage III. Conclusions: Results of our study suggest that melanoma is oxidative stress associated disease, as well as deteriorated cell functioning at mitochondrial level.",
publisher = "Društvo medicinskih biohemičara Srbije, Beograd i Versita",
journal = "Journal of Medical Biochemistry",
title = "Association between oxidative stress and melanoma progression",
volume = "37",
number = "1",
pages = "12-20",
doi = "10.1515/jomb-2017-0040"
}

Pantić Biševac, J., Đukić, M., Stanojević, I., Stevanović, I., Mijušković, Ž., Đurić, A., Gobeljić, B., Banović, T.,& Vojvodić, D.. (2018). Association between oxidative stress and melanoma progression. in Journal of Medical Biochemistry
Društvo medicinskih biohemičara Srbije, Beograd i Versita., 37(1), 12-20.
https://doi.org/10.1515/jomb-2017-0040

Pantić Biševac J, Đukić M, Stanojević I, Stevanović I, Mijušković Ž, Đurić A, Gobeljić B, Banović T, Vojvodić D. Association between oxidative stress and melanoma progression. in Journal of Medical Biochemistry. 2018;37(1):12-20.
doi:10.1515/jomb-2017-0040 .

Pantić Biševac, Jelena, Đukić, Mirjana, Stanojević, Ivan, Stevanović, Ivana, Mijušković, Željko, Đurić, Ana, Gobeljić, Borko, Banović, Tatjana, Vojvodić, Danilo, "Association between oxidative stress and melanoma progression" in Journal of Medical Biochemistry, 37, no. 1 (2018):12-20,
https://doi.org/10.1515/jomb-2017-0040 . .

TY  - JOUR
AU  - Đurić, Ana
AU  - Begić, Aida
AU  - Gobeljić, Borko
AU  - Pantelić, Ana
AU  - Zebić, Goran
AU  - Stevanović, Ivana
AU  - Đurđević, Dragan
AU  - Ninković, Milica
AU  - Prokić, Vera
AU  - Stanojević, Ivan
AU  - Vojvodić, Danilo
AU  - Đukić, Mirjana
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2969
AB  - The aim of the study was to investigate if alcohol and disulfiram (DSF) individually and in combination affect bioelements' and red-ox homeostasis in testes of the exposed rats. The animals were divided into groups according to the duration of treatments (21 and/or 42 days): C-21/C-42 groups (controls); OL21 and OL22-42 groups (0.5 mL olive oil intake); A(1-21) groups (3 mL 20% ethanol intake); DSF1-21 groups (178.5 mg DSF/kg/day intake); and A(21)+DSF22-42 groups (the DSF ingestion followed previous 21 days' treatment with alcohol). The measured parameters in testes included metals: zinc (Zn), copper (Cu), iron (Fe), magnesium (Mg) and selenium (Se); as well as oxidative stress (OS) parameters: superoxide anion radical (O-2(center dot-)), glutathione reduced (GSH) and oxidized (GSSG), malondialdehyde (MDA), hydrogen peroxide (H2O2) decomposition and activities of total superoxide dismutase (tSOD), glutathione-Stransferase (GST) and glutathione reductase (GR). Metal status was changed in all experimental groups (Fe rose, Zn fell, while Cu increased in A(21)+DSF24-32 groups). Development of OS was demonstrated in A(1-21) groups, but not in DSF1-21 groups. In A(21)+DSF22-42 groups, OS was partially reduced compared to A groups (A(1-21)>MDA>C; A(1-21) lt GSH lt C). High metal-binding affinity of DSF/DDTC changes red-ox homeostasis in rat testes.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Food and Chemical Toxicology
T1  - Subacute alcohol and/or disulfiram intake affects bioelements and redox status in rat testes
VL  - 105
SP  - 44
EP  - 51
DO  - 10.1016/j.fct.2017.03.041
ER  - 

@article{
author = "Đurić, Ana and Begić, Aida and Gobeljić, Borko and Pantelić, Ana and Zebić, Goran and Stevanović, Ivana and Đurđević, Dragan and Ninković, Milica and Prokić, Vera and Stanojević, Ivan and Vojvodić, Danilo and Đukić, Mirjana",
year = "2017",
abstract = "The aim of the study was to investigate if alcohol and disulfiram (DSF) individually and in combination affect bioelements' and red-ox homeostasis in testes of the exposed rats. The animals were divided into groups according to the duration of treatments (21 and/or 42 days): C-21/C-42 groups (controls); OL21 and OL22-42 groups (0.5 mL olive oil intake); A(1-21) groups (3 mL 20% ethanol intake); DSF1-21 groups (178.5 mg DSF/kg/day intake); and A(21)+DSF22-42 groups (the DSF ingestion followed previous 21 days' treatment with alcohol). The measured parameters in testes included metals: zinc (Zn), copper (Cu), iron (Fe), magnesium (Mg) and selenium (Se); as well as oxidative stress (OS) parameters: superoxide anion radical (O-2(center dot-)), glutathione reduced (GSH) and oxidized (GSSG), malondialdehyde (MDA), hydrogen peroxide (H2O2) decomposition and activities of total superoxide dismutase (tSOD), glutathione-Stransferase (GST) and glutathione reductase (GR). Metal status was changed in all experimental groups (Fe rose, Zn fell, while Cu increased in A(21)+DSF24-32 groups). Development of OS was demonstrated in A(1-21) groups, but not in DSF1-21 groups. In A(21)+DSF22-42 groups, OS was partially reduced compared to A groups (A(1-21)>MDA>C; A(1-21) lt GSH lt C). High metal-binding affinity of DSF/DDTC changes red-ox homeostasis in rat testes.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Food and Chemical Toxicology",
title = "Subacute alcohol and/or disulfiram intake affects bioelements and redox status in rat testes",
volume = "105",
pages = "44-51",
doi = "10.1016/j.fct.2017.03.041"
}

Đurić, A., Begić, A., Gobeljić, B., Pantelić, A., Zebić, G., Stevanović, I., Đurđević, D., Ninković, M., Prokić, V., Stanojević, I., Vojvodić, D.,& Đukić, M.. (2017). Subacute alcohol and/or disulfiram intake affects bioelements and redox status in rat testes. in Food and Chemical Toxicology
Pergamon-Elsevier Science Ltd, Oxford., 105, 44-51.
https://doi.org/10.1016/j.fct.2017.03.041

Đurić A, Begić A, Gobeljić B, Pantelić A, Zebić G, Stevanović I, Đurđević D, Ninković M, Prokić V, Stanojević I, Vojvodić D, Đukić M. Subacute alcohol and/or disulfiram intake affects bioelements and redox status in rat testes. in Food and Chemical Toxicology. 2017;105:44-51.
doi:10.1016/j.fct.2017.03.041 .

Đurić, Ana, Begić, Aida, Gobeljić, Borko, Pantelić, Ana, Zebić, Goran, Stevanović, Ivana, Đurđević, Dragan, Ninković, Milica, Prokić, Vera, Stanojević, Ivan, Vojvodić, Danilo, Đukić, Mirjana, "Subacute alcohol and/or disulfiram intake affects bioelements and redox status in rat testes" in Food and Chemical Toxicology, 105 (2017):44-51,
https://doi.org/10.1016/j.fct.2017.03.041 . .

TY  - JOUR
AU  - Begić, Aida
AU  - Đurić, Ana
AU  - Ninković, Milica
AU  - Stevanović, Ivana
AU  - Đurđević, Dragan
AU  - Pavlović, Miloš
AU  - Jelić, Katarina
AU  - Pantelić, Ana
AU  - Zebić, Goran
AU  - Dejanović, Bratislav
AU  - Stanojević, Ivan
AU  - Vojvodić, Danilo
AU  - Milosavljević, Petar
AU  - Đukić, Mirjana
AU  - Saso, Luciano
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2963
AB  - Examination of cadmium (Cd) toxicity and disulfiram (DSF) effect on liver was focused on oxidative stress (OS), bioelements status, morphological and functional changes. Male Wistar rats were intraperitoneally treated with 1mg CdCl2/kg BW/day; orally with 178.5 mg DSF/kg BW/day for 1, 3, 10 and 21 days; and co-exposed from 22nd to 42nd day. The co-exposure nearly restored previously suppressed total superoxide dismutase (SOD), catalase (CAT) and increased glutathione peroxidase (GPx) activities; increased previously reduced glutathione reductase (GR) and total glutathione-S-transferase (GST) activities; reduced previously increased superoxide anion radical (O-2(center dot-)) and malondialdehyde (MDA) levels; increased zinc (Zn) and iron (Fe), and decreased copper (Cu) (yet above control value), while magnesium (Mg) was not affected; and decreased serum alanine aminotransferases (ALT) levels. Histopathological examination showed signs of inflammation process as previously demonstrated by exposure to Cd. Overall, we ascertained partial liver redox status improvement, compared with the formerly Cd-induced impact.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Journal of Enzyme Inhibition and Medicinal Chemistry
T1  - Disulfiram moderately restores impaired hepatic redox status of rats subchronically exposed to cadmium
VL  - 32
IS  - 1
SP  - 478
EP  - 489
DO  - 10.1080/14756366.2016.1261132
ER  - 

@article{
author = "Begić, Aida and Đurić, Ana and Ninković, Milica and Stevanović, Ivana and Đurđević, Dragan and Pavlović, Miloš and Jelić, Katarina and Pantelić, Ana and Zebić, Goran and Dejanović, Bratislav and Stanojević, Ivan and Vojvodić, Danilo and Milosavljević, Petar and Đukić, Mirjana and Saso, Luciano",
year = "2017",
abstract = "Examination of cadmium (Cd) toxicity and disulfiram (DSF) effect on liver was focused on oxidative stress (OS), bioelements status, morphological and functional changes. Male Wistar rats were intraperitoneally treated with 1mg CdCl2/kg BW/day; orally with 178.5 mg DSF/kg BW/day for 1, 3, 10 and 21 days; and co-exposed from 22nd to 42nd day. The co-exposure nearly restored previously suppressed total superoxide dismutase (SOD), catalase (CAT) and increased glutathione peroxidase (GPx) activities; increased previously reduced glutathione reductase (GR) and total glutathione-S-transferase (GST) activities; reduced previously increased superoxide anion radical (O-2(center dot-)) and malondialdehyde (MDA) levels; increased zinc (Zn) and iron (Fe), and decreased copper (Cu) (yet above control value), while magnesium (Mg) was not affected; and decreased serum alanine aminotransferases (ALT) levels. Histopathological examination showed signs of inflammation process as previously demonstrated by exposure to Cd. Overall, we ascertained partial liver redox status improvement, compared with the formerly Cd-induced impact.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Journal of Enzyme Inhibition and Medicinal Chemistry",
title = "Disulfiram moderately restores impaired hepatic redox status of rats subchronically exposed to cadmium",
volume = "32",
number = "1",
pages = "478-489",
doi = "10.1080/14756366.2016.1261132"
}

Begić, A., Đurić, A., Ninković, M., Stevanović, I., Đurđević, D., Pavlović, M., Jelić, K., Pantelić, A., Zebić, G., Dejanović, B., Stanojević, I., Vojvodić, D., Milosavljević, P., Đukić, M.,& Saso, L.. (2017). Disulfiram moderately restores impaired hepatic redox status of rats subchronically exposed to cadmium. in Journal of Enzyme Inhibition and Medicinal Chemistry
Taylor & Francis Ltd, Abingdon., 32(1), 478-489.
https://doi.org/10.1080/14756366.2016.1261132

Begić A, Đurić A, Ninković M, Stevanović I, Đurđević D, Pavlović M, Jelić K, Pantelić A, Zebić G, Dejanović B, Stanojević I, Vojvodić D, Milosavljević P, Đukić M, Saso L. Disulfiram moderately restores impaired hepatic redox status of rats subchronically exposed to cadmium. in Journal of Enzyme Inhibition and Medicinal Chemistry. 2017;32(1):478-489.
doi:10.1080/14756366.2016.1261132 .

Begić, Aida, Đurić, Ana, Ninković, Milica, Stevanović, Ivana, Đurđević, Dragan, Pavlović, Miloš, Jelić, Katarina, Pantelić, Ana, Zebić, Goran, Dejanović, Bratislav, Stanojević, Ivan, Vojvodić, Danilo, Milosavljević, Petar, Đukić, Mirjana, Saso, Luciano, "Disulfiram moderately restores impaired hepatic redox status of rats subchronically exposed to cadmium" in Journal of Enzyme Inhibition and Medicinal Chemistry, 32, no. 1 (2017):478-489,
https://doi.org/10.1080/14756366.2016.1261132 . .

TY  - JOUR
AU  - Begić, Aida
AU  - Đurić, Ana
AU  - Gobeljić, Borko
AU  - Stevanović, Ivana
AU  - Lukić, Vera
AU  - Stanojević, Ivan
AU  - Ninković, Milica
AU  - Saso, Luciano
AU  - Vojvodić, Danilo
AU  - Đukić, Mirjana
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2952
AB  - The aim of our work was to optimize and apply simple high-performance liquid chromatography method with ultraviolet detection (HPLC-UV) for simultaneous determination of reduced (GSH) and oxidized (GSSG) glutathione in biological matrix (specifically, the rat liver tissue was used herein), since the ratio between oxidized and reduced glutathione forms (GSSG-GSH) has been recognized as an important biological marker of oxidatively depleted GSH in oxidative stress (OS)-associated diseases and poisonings. An isocratic chromatographic separation of GSH and GSSG (2.8 min and 6.3 min, respectively) was performed with the mobile phase consisted of sodium perchlorate solution (pH adjusted to 2.8) at flow rate of 1 mL min(-1), detection set at 215 nm, and column temperature of 40 degrees C. The method offers short run time, linearity in the range of 0.01-200 mu M concentration for both compounds (R-2 = 1), low limits of detection and quantification (GSH: 0.18 mu M and 0.56 mu M, GSSG: 0.52 mu M and 1.58 mu M, respectively), precision, accuracy (bias  lt 2%), and high reproducibility. Through suitable sample handling, an overestimation of GSSG was prevented. High recovery (>99%) was achieved. The method was successfully applied for the analysis of GSH and GSSG in liver homogenates of Wistar rats intraperitoneally exposed to cadmium (Cd) (1 mg kg(-1) CdCl2/21 days). Regardless of other Cd-mediated hepatotoxicity mechanisms, herein, we have exclusively interpreted/emphasized oxidative GSH depletion. The presented method is acceptable for a routine analysis of GSH and GSSG in biological matrix, while the calculated ratio GSSG-GSH is considered as a valuable OS marker.
PB  - Akademiai Kiado Rt, Budapest
T2  - Acta Chromatographica
T1  - The Simple Isocratic HPLC-UV Method for the Simultaneous Determination of Reduced and Oxidized Glutathione in Animal Tissue
VL  - 29
IS  - 1
SP  - 67
EP  - 84
DO  - 10.1556/1326.2017.29.1.5
ER  - 

@article{
author = "Begić, Aida and Đurić, Ana and Gobeljić, Borko and Stevanović, Ivana and Lukić, Vera and Stanojević, Ivan and Ninković, Milica and Saso, Luciano and Vojvodić, Danilo and Đukić, Mirjana",
year = "2017",
abstract = "The aim of our work was to optimize and apply simple high-performance liquid chromatography method with ultraviolet detection (HPLC-UV) for simultaneous determination of reduced (GSH) and oxidized (GSSG) glutathione in biological matrix (specifically, the rat liver tissue was used herein), since the ratio between oxidized and reduced glutathione forms (GSSG-GSH) has been recognized as an important biological marker of oxidatively depleted GSH in oxidative stress (OS)-associated diseases and poisonings. An isocratic chromatographic separation of GSH and GSSG (2.8 min and 6.3 min, respectively) was performed with the mobile phase consisted of sodium perchlorate solution (pH adjusted to 2.8) at flow rate of 1 mL min(-1), detection set at 215 nm, and column temperature of 40 degrees C. The method offers short run time, linearity in the range of 0.01-200 mu M concentration for both compounds (R-2 = 1), low limits of detection and quantification (GSH: 0.18 mu M and 0.56 mu M, GSSG: 0.52 mu M and 1.58 mu M, respectively), precision, accuracy (bias  lt 2%), and high reproducibility. Through suitable sample handling, an overestimation of GSSG was prevented. High recovery (>99%) was achieved. The method was successfully applied for the analysis of GSH and GSSG in liver homogenates of Wistar rats intraperitoneally exposed to cadmium (Cd) (1 mg kg(-1) CdCl2/21 days). Regardless of other Cd-mediated hepatotoxicity mechanisms, herein, we have exclusively interpreted/emphasized oxidative GSH depletion. The presented method is acceptable for a routine analysis of GSH and GSSG in biological matrix, while the calculated ratio GSSG-GSH is considered as a valuable OS marker.",
publisher = "Akademiai Kiado Rt, Budapest",
journal = "Acta Chromatographica",
title = "The Simple Isocratic HPLC-UV Method for the Simultaneous Determination of Reduced and Oxidized Glutathione in Animal Tissue",
volume = "29",
number = "1",
pages = "67-84",
doi = "10.1556/1326.2017.29.1.5"
}

Begić, A., Đurić, A., Gobeljić, B., Stevanović, I., Lukić, V., Stanojević, I., Ninković, M., Saso, L., Vojvodić, D.,& Đukić, M.. (2017). The Simple Isocratic HPLC-UV Method for the Simultaneous Determination of Reduced and Oxidized Glutathione in Animal Tissue. in Acta Chromatographica
Akademiai Kiado Rt, Budapest., 29(1), 67-84.
https://doi.org/10.1556/1326.2017.29.1.5

Begić A, Đurić A, Gobeljić B, Stevanović I, Lukić V, Stanojević I, Ninković M, Saso L, Vojvodić D, Đukić M. The Simple Isocratic HPLC-UV Method for the Simultaneous Determination of Reduced and Oxidized Glutathione in Animal Tissue. in Acta Chromatographica. 2017;29(1):67-84.
doi:10.1556/1326.2017.29.1.5 .

Begić, Aida, Đurić, Ana, Gobeljić, Borko, Stevanović, Ivana, Lukić, Vera, Stanojević, Ivan, Ninković, Milica, Saso, Luciano, Vojvodić, Danilo, Đukić, Mirjana, "The Simple Isocratic HPLC-UV Method for the Simultaneous Determination of Reduced and Oxidized Glutathione in Animal Tissue" in Acta Chromatographica, 29, no. 1 (2017):67-84,
https://doi.org/10.1556/1326.2017.29.1.5 . .

TY  - JOUR
AU  - Mancić, Bojana
AU  - Stevanović, Ivana
AU  - Ilić, Tihomir V.
AU  - Đurić, Ana
AU  - Stojanović, Ivana
AU  - Milanović, Slađan
AU  - Ninković, Milica
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2639
AB  - Repetitive transcranial magnetic stimulation (rTMS) induces changes in expression of proteins engaged in activity of excitatory and inhibitory systems as well as redox homeostasis. Our aim was to investigate the effect of single (SS) and repeated session (RS) of intermittentand continuous theta-burst stimulation (iTBS; cTBS) on the expression of vesicular and plasmatic glutamate transporters 1 (vGluT1 and GLT-1), glial fibrillary acidic protein (GFAP) and influence on oxidative status in rats cerebellar tissue and plasma. Redox state parameters in cerebellar tissue and plasma were assessed 24 h after single and 48 h after the last TBS session. Molecular changes were examined by immunofluorescence. Stimulation significantly increased thiol groups (SH) in tissue of SS iTBS group, and decreased in iTBS RS. Activity of glucose-6-phosphate-dehydrogenase (G6PD) was increased markedly in cTBS RS. Immunoreactivity of vGluT1 in cTBS RS decreased, while GLT-1 increased in cTBS SS and cTBS RS, compared to control. Present study gives insight in molecular and biochemical mechanisms by which iTBS and cTBS exerts its effects on rats cerebellar cortex.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Neurochemistry International
T1  - Transcranial theta-burst stimulation alters GLT-1 and vGluT1 expression in rat cerebellar cortex
VL  - 100
SP  - 120
EP  - 127
DO  - 10.1016/j.neuint.2016.09.009
ER  - 

@article{
author = "Mancić, Bojana and Stevanović, Ivana and Ilić, Tihomir V. and Đurić, Ana and Stojanović, Ivana and Milanović, Slađan and Ninković, Milica",
year = "2016",
abstract = "Repetitive transcranial magnetic stimulation (rTMS) induces changes in expression of proteins engaged in activity of excitatory and inhibitory systems as well as redox homeostasis. Our aim was to investigate the effect of single (SS) and repeated session (RS) of intermittentand continuous theta-burst stimulation (iTBS; cTBS) on the expression of vesicular and plasmatic glutamate transporters 1 (vGluT1 and GLT-1), glial fibrillary acidic protein (GFAP) and influence on oxidative status in rats cerebellar tissue and plasma. Redox state parameters in cerebellar tissue and plasma were assessed 24 h after single and 48 h after the last TBS session. Molecular changes were examined by immunofluorescence. Stimulation significantly increased thiol groups (SH) in tissue of SS iTBS group, and decreased in iTBS RS. Activity of glucose-6-phosphate-dehydrogenase (G6PD) was increased markedly in cTBS RS. Immunoreactivity of vGluT1 in cTBS RS decreased, while GLT-1 increased in cTBS SS and cTBS RS, compared to control. Present study gives insight in molecular and biochemical mechanisms by which iTBS and cTBS exerts its effects on rats cerebellar cortex.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Neurochemistry International",
title = "Transcranial theta-burst stimulation alters GLT-1 and vGluT1 expression in rat cerebellar cortex",
volume = "100",
pages = "120-127",
doi = "10.1016/j.neuint.2016.09.009"
}

Mancić, B., Stevanović, I., Ilić, T. V., Đurić, A., Stojanović, I., Milanović, S.,& Ninković, M.. (2016). Transcranial theta-burst stimulation alters GLT-1 and vGluT1 expression in rat cerebellar cortex. in Neurochemistry International
Pergamon-Elsevier Science Ltd, Oxford., 100, 120-127.
https://doi.org/10.1016/j.neuint.2016.09.009

Mancić B, Stevanović I, Ilić TV, Đurić A, Stojanović I, Milanović S, Ninković M. Transcranial theta-burst stimulation alters GLT-1 and vGluT1 expression in rat cerebellar cortex. in Neurochemistry International. 2016;100:120-127.
doi:10.1016/j.neuint.2016.09.009 .

Mancić, Bojana, Stevanović, Ivana, Ilić, Tihomir V., Đurić, Ana, Stojanović, Ivana, Milanović, Slađan, Ninković, Milica, "Transcranial theta-burst stimulation alters GLT-1 and vGluT1 expression in rat cerebellar cortex" in Neurochemistry International, 100 (2016):120-127,
https://doi.org/10.1016/j.neuint.2016.09.009 . .

TY  - JOUR
AU  - Đurđević, Dragan
AU  - Đukić, Mirjana
AU  - Ninković, Milica
AU  - Stevanović, Ivana
AU  - Jovanović, Marina
AU  - Vasić, Una
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2062
AB  - Diquat (DQ) neurotoxicity mechanisms are unknown, although, it's systemic toxicity is mediated by free radical reactions. The role of glutathione cycle was assessed by glutathione reductase (GR) applied in the pre-treatment of DQ poisoning. Wistar rats were used and tested compounds were administered intrastriatally (i.s.) in one single dose. Total glutathione (tGSH), glutathione disulfide (GSSG) and glutathione peroxidase (GPx) were measured in the vulnerable brain regions (VBRs) (striatum, hippocampus and cortex), at 30 minutes, 24 hours and 7 days post treatment. Results from the intact and the sham operated groups were not statistically different. Rapid spatial spreading of oxidative stress was confirmed in the examined VBRs.. Mortality (30-40%, within 24hrs) and signs of lethargy were observed in the DQ group. Activity of GPx activity was elevated and GSSG/GSH was higher in the examined VBRs during the experiment, compared to the controls. The i.s. pre-treatment with GR achieved neuroprotective role against DQ induced neurotoxicity, based on animal survival, absence of lethargy and decreased GPx activity and GSSG/GSH in the examined VBRs during the experiment, compared to the DQ group. Our results confirmed that oxidation of GSH was the reason for the reduced antioxidative defense against DQ neurotoxicity.
AB  - Mehanizmi neurotoksičnosti dikvata (DK) su nepoznati, mada se zna da je sistemska toksičnost posredovana reakcijama slobodnih radikala. Uloga glutationskog ciklusa je isptivana primenom glutation reduktaze (GR) u predtretmanu trovanja DK. Wistar pacovi su korišćeni i testirana jedinjenja intrastrjiatalno (i.s.) primenjena u jednokratnoj dozi. Ukupni glutation (tGSH), glutation-disulfid (GSSG) i aktivnost glutation peroksidaze (GPx) su mereni u selektivno osetljivim regionima mozga (strijatum, hipokampus i korteks), 30. minuta, 24. sata i 7. dana posle tretmana. Rezultati netretiranih (intaktna grupa) i lažno operisanih pacova se ne razlikuju statistički. Vremensko i prostorno širenje oksidativnog stresa je potvrđeno kod ispitivanih moždanih struktura. Mortalitet (30-40%, u roku od 24 casa) i znaci letargije su uočeni u samo u DK grupi. Statistički povećana aktivnost GPx, kao i odnosa GSSG/GSH u ispitivanim moždanim strukturama tokom eksperimenta, potvrđuje oksidativno narušenu ravnotežu i oštećenja moždanog tkiva. Predtretman i.s. sa GR je ispoljio neurozaštitni efekat od neurotoksičnosti DK, bazirano na preživljavanju životinja, odsustvu letargije i smanjenoj aktivnost GPx i odnosa GSSG / GSH ispitivanih moždanih struktura tokom eksperimenta, u odnosu na DK grupu. Naši rezultati ukazuju da je oksidacija GSH kljucna za smanjenje antioksidativne odbrane od DK neurotoksicnosti.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta veterinaria
T1  - Glutathione cycle in diquat neurotoxicity: Assessed by intrastriatal pre-treatment with glutathione reductase
T1  - Uloga glutationskog ciklusa u neurotoksičnosti dikvata - ispitivano primenom intrastrijatalnog predtretmana sa glutation reduktazom
VL  - 63
IS  - 2-3
SP  - 159
EP  - 175
DO  - 10.2298/AVB1303159D
ER  - 

@article{
author = "Đurđević, Dragan and Đukić, Mirjana and Ninković, Milica and Stevanović, Ivana and Jovanović, Marina and Vasić, Una",
year = "2013",
abstract = "Diquat (DQ) neurotoxicity mechanisms are unknown, although, it's systemic toxicity is mediated by free radical reactions. The role of glutathione cycle was assessed by glutathione reductase (GR) applied in the pre-treatment of DQ poisoning. Wistar rats were used and tested compounds were administered intrastriatally (i.s.) in one single dose. Total glutathione (tGSH), glutathione disulfide (GSSG) and glutathione peroxidase (GPx) were measured in the vulnerable brain regions (VBRs) (striatum, hippocampus and cortex), at 30 minutes, 24 hours and 7 days post treatment. Results from the intact and the sham operated groups were not statistically different. Rapid spatial spreading of oxidative stress was confirmed in the examined VBRs.. Mortality (30-40%, within 24hrs) and signs of lethargy were observed in the DQ group. Activity of GPx activity was elevated and GSSG/GSH was higher in the examined VBRs during the experiment, compared to the controls. The i.s. pre-treatment with GR achieved neuroprotective role against DQ induced neurotoxicity, based on animal survival, absence of lethargy and decreased GPx activity and GSSG/GSH in the examined VBRs during the experiment, compared to the DQ group. Our results confirmed that oxidation of GSH was the reason for the reduced antioxidative defense against DQ neurotoxicity., Mehanizmi neurotoksičnosti dikvata (DK) su nepoznati, mada se zna da je sistemska toksičnost posredovana reakcijama slobodnih radikala. Uloga glutationskog ciklusa je isptivana primenom glutation reduktaze (GR) u predtretmanu trovanja DK. Wistar pacovi su korišćeni i testirana jedinjenja intrastrjiatalno (i.s.) primenjena u jednokratnoj dozi. Ukupni glutation (tGSH), glutation-disulfid (GSSG) i aktivnost glutation peroksidaze (GPx) su mereni u selektivno osetljivim regionima mozga (strijatum, hipokampus i korteks), 30. minuta, 24. sata i 7. dana posle tretmana. Rezultati netretiranih (intaktna grupa) i lažno operisanih pacova se ne razlikuju statistički. Vremensko i prostorno širenje oksidativnog stresa je potvrđeno kod ispitivanih moždanih struktura. Mortalitet (30-40%, u roku od 24 casa) i znaci letargije su uočeni u samo u DK grupi. Statistički povećana aktivnost GPx, kao i odnosa GSSG/GSH u ispitivanim moždanim strukturama tokom eksperimenta, potvrđuje oksidativno narušenu ravnotežu i oštećenja moždanog tkiva. Predtretman i.s. sa GR je ispoljio neurozaštitni efekat od neurotoksičnosti DK, bazirano na preživljavanju životinja, odsustvu letargije i smanjenoj aktivnost GPx i odnosa GSSG / GSH ispitivanih moždanih struktura tokom eksperimenta, u odnosu na DK grupu. Naši rezultati ukazuju da je oksidacija GSH kljucna za smanjenje antioksidativne odbrane od DK neurotoksicnosti.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta veterinaria",
title = "Glutathione cycle in diquat neurotoxicity: Assessed by intrastriatal pre-treatment with glutathione reductase, Uloga glutationskog ciklusa u neurotoksičnosti dikvata - ispitivano primenom intrastrijatalnog predtretmana sa glutation reduktazom",
volume = "63",
number = "2-3",
pages = "159-175",
doi = "10.2298/AVB1303159D"
}

Đurđević, D., Đukić, M., Ninković, M., Stevanović, I., Jovanović, M.,& Vasić, U.. (2013). Glutathione cycle in diquat neurotoxicity: Assessed by intrastriatal pre-treatment with glutathione reductase. in Acta veterinaria
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 63(2-3), 159-175.
https://doi.org/10.2298/AVB1303159D

Đurđević D, Đukić M, Ninković M, Stevanović I, Jovanović M, Vasić U. Glutathione cycle in diquat neurotoxicity: Assessed by intrastriatal pre-treatment with glutathione reductase. in Acta veterinaria. 2013;63(2-3):159-175.
doi:10.2298/AVB1303159D .

Đurđević, Dragan, Đukić, Mirjana, Ninković, Milica, Stevanović, Ivana, Jovanović, Marina, Vasić, Una, "Glutathione cycle in diquat neurotoxicity: Assessed by intrastriatal pre-treatment with glutathione reductase" in Acta veterinaria, 63, no. 2-3 (2013):159-175,
https://doi.org/10.2298/AVB1303159D . .

TY  - JOUR
AU  - Đukić, Mirjana
AU  - Jovanović, Marina
AU  - Ninković, Milica
AU  - Stevanović, Ivana
AU  - Ćurčić, Marijana
AU  - Topić, Aleksandra
AU  - Vujanović, Dragana
AU  - Đurđević, Dragan
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1663
AB  - Introduction: Contact herbicide diquat (DQ), redox cycling compound, mediates its systemic toxicity throughout the enlarged production of free radicals. Target organs are liver and kidney in humans. To-date, the mechanism of DQ-induced neurotoxicity has not been rationalized. Objective: The objectives of the study were to examine the ability of DQ to induce oxidative stress (OS) and/or nitrosative stress (NS) upon intrastriatal (i.s.) administration and to investigate the role of nitric oxide (NOx) using NG-nitro-L-arginine methyl ester (L-NAME), a non-selective inhibitor of nitric oxide synthase (NOS) in the pretreatment of DQ i.s. administration. Material and Methods: The experiment was conducted on Wistar rats, randomly divided in experimental groups, receiving different treatments i.s. applied. Parameters of OS/NS such as: superoxide anion radical (O-2(center dot-)), superoxide dismutase (SOD), malondialdehyde (MDA) and nitrates (NO3-) were measured in the cortex (bilaterally), at 30th min, 24 hours and 7 days after the treatments. Results: Lethargy and high mortality rate were observed only in the DQ group (within 24 hours and 2-3 hours, respectively) after awakening from anesthesia. Markedly increased production of NOx and O-2(center dot-) along with elevated lipid peroxidation altogether contributed to DQ neurotoxicity. The most importantly, the L-NAME i.s. pretreatment protected treated animals from dying and diminished OS/NS response against DQ-induced neurotoxicity. Conclusion: The i.s. pretreatment with L-NAME resulted in neuroprotection against DQ neurotoxity, based on animal survival and reduced LPO in the cortex.
PB  - Inst Agricultural Medicine, Lublin
T2  - Annals of Agricultural and Environmental Medicine
T1  - Intrastriatal pre-treatment with L-NAME protects rats from diquat neurotoxcity
VL  - 19
IS  - 4
SP  - 666
EP  - 672
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1663
ER  - 

@article{
author = "Đukić, Mirjana and Jovanović, Marina and Ninković, Milica and Stevanović, Ivana and Ćurčić, Marijana and Topić, Aleksandra and Vujanović, Dragana and Đurđević, Dragan",
year = "2012",
abstract = "Introduction: Contact herbicide diquat (DQ), redox cycling compound, mediates its systemic toxicity throughout the enlarged production of free radicals. Target organs are liver and kidney in humans. To-date, the mechanism of DQ-induced neurotoxicity has not been rationalized. Objective: The objectives of the study were to examine the ability of DQ to induce oxidative stress (OS) and/or nitrosative stress (NS) upon intrastriatal (i.s.) administration and to investigate the role of nitric oxide (NOx) using NG-nitro-L-arginine methyl ester (L-NAME), a non-selective inhibitor of nitric oxide synthase (NOS) in the pretreatment of DQ i.s. administration. Material and Methods: The experiment was conducted on Wistar rats, randomly divided in experimental groups, receiving different treatments i.s. applied. Parameters of OS/NS such as: superoxide anion radical (O-2(center dot-)), superoxide dismutase (SOD), malondialdehyde (MDA) and nitrates (NO3-) were measured in the cortex (bilaterally), at 30th min, 24 hours and 7 days after the treatments. Results: Lethargy and high mortality rate were observed only in the DQ group (within 24 hours and 2-3 hours, respectively) after awakening from anesthesia. Markedly increased production of NOx and O-2(center dot-) along with elevated lipid peroxidation altogether contributed to DQ neurotoxicity. The most importantly, the L-NAME i.s. pretreatment protected treated animals from dying and diminished OS/NS response against DQ-induced neurotoxicity. Conclusion: The i.s. pretreatment with L-NAME resulted in neuroprotection against DQ neurotoxity, based on animal survival and reduced LPO in the cortex.",
publisher = "Inst Agricultural Medicine, Lublin",
journal = "Annals of Agricultural and Environmental Medicine",
title = "Intrastriatal pre-treatment with L-NAME protects rats from diquat neurotoxcity",
volume = "19",
number = "4",
pages = "666-672",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1663"
}

Đukić, M., Jovanović, M., Ninković, M., Stevanović, I., Ćurčić, M., Topić, A., Vujanović, D.,& Đurđević, D.. (2012). Intrastriatal pre-treatment with L-NAME protects rats from diquat neurotoxcity. in Annals of Agricultural and Environmental Medicine
Inst Agricultural Medicine, Lublin., 19(4), 666-672.
https://hdl.handle.net/21.15107/rcub_farfar_1663

Đukić M, Jovanović M, Ninković M, Stevanović I, Ćurčić M, Topić A, Vujanović D, Đurđević D. Intrastriatal pre-treatment with L-NAME protects rats from diquat neurotoxcity. in Annals of Agricultural and Environmental Medicine. 2012;19(4):666-672.
https://hdl.handle.net/21.15107/rcub_farfar_1663 .

Đukić, Mirjana, Jovanović, Marina, Ninković, Milica, Stevanović, Ivana, Ćurčić, Marijana, Topić, Aleksandra, Vujanović, Dragana, Đurđević, Dragan, "Intrastriatal pre-treatment with L-NAME protects rats from diquat neurotoxcity" in Annals of Agricultural and Environmental Medicine, 19, no. 4 (2012):666-672,
https://hdl.handle.net/21.15107/rcub_farfar_1663 .

TY  - JOUR
AU  - Đukić, Mirjana
AU  - Jovanović, Marina
AU  - Ninković, Milica
AU  - Stevanović, Ivana
AU  - Đurđević, Dragan
AU  - Vasić, Una
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1792
AB  - In this study we examined if the response of the cortex against diquat (DQ), intrastriatally (i.s.) applied to Wistar rats, was mediated by oxidative/nitrosative stress (OS/NS). In particular, we were focused on the glutathione (GSH) antioxidative role, thus we applied i.s. glutathione reductase (GR) in the pre-treatment of DQ administration. Superoxide anion radical (O2 •-), nitrate (NO3 -), malondialdehyde (MDA) and superoxide dismutase (SOD), were measured in ipsi- and contra- lateral sides of the cortex, at 30 minutes, 24 hours and 7 days post treatment. The redox balance was not significantly changed in the cortex of sham operated and intact groups. Also, no differences were observed between the ipsi- and contra- lateral side of the cortex. Lethargy and mortality (30-40%) of the animals in the DQ group within 24 hrs, coincided with rapidly developed lipid peroxidation supported by OS/NS upon i.s. DQ administration. Strong redox potential of DQ probably resulted in a huge deprivation of molecular oxygen. The pretreatment with GR acted neuro-protectively, based on animal survival and absence of lethargy, although, lipid peroxidation was not developed in the GR+DQ group, OS was documented by a high concentration of O2 •- (within 24 hrs), descending and eventually inhibiting SOD activity (at 7 days).
AB  - U ovoj studiji smo ispitali da li je oksidativni/nitrosativni stres (OS/NS), uključen u odgovor korteksa Wistar pacova nakon intrastrijatalne (i.s.) izloženosti dikvatu (DK). Posebno smo ispitivali značaj antioksidativne uloge glutationa (GSH), zbog čega smo primenili glutation reduktazu (GR) u predtretmanu davanja DK. Superoksid anjon radikal (O2•¯), nitrati (NO3¯), malondialdehid (MDA) i superoksid dismutaza (SOD), su mereni u obostranom korteksu (ipsi- i kontrastrana), nakon 30 minuta, 24 sati i 7 dana od tretmana. Redoks balans se nije značajno promenio u korteksu lažno operisanih i netretiranih pacova. Takođe, ne postoji statistički značajna razlika između ipsi- i kontra- strane korteksa. Letargija i mortalitet (30-40%) kod životinja u DK grupi su uočene tokom 24 časa od i.s. trovanja DK, što se poklopilo sa naglim razvojem OS/NS i lipidne peroksidacije. Visok redoks potencijal DK verovatno rezultira opsežnim utroškom molekularnog kiseonika. Zaključeno je da je ostvaren neuroprotektivni učinak predtretmana sa GR, na osnovu preživljavanja životinja i odsustva letargije. Lipidna peroksidacija nije bila razvijena u grupi predtretiranoj sa GR ali je ipak izmerena visoka koncentracija O2•¯ (tokom 24 sata) koja zatim opada i na kraju 7. dana u potpunosti inhibira aktivnost SOD.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta veterinaria
T1  - Influence of glutathione reductase on diquat neurotoxcity assessed by oxidative/nitrosative stress in the cortex of intrastriatally treated rats
T1  - Uticaj glutation reduktaze na neurotoksičnost dikvata - ispitivan je oksidativni/nitrozativni stres u korteksu intrastrijatalno tretiranih pacova
VL  - 62
IS  - 5-6
SP  - 553
EP  - 568
DO  - 10.2298/AVB1206553D
ER  - 

@article{
author = "Đukić, Mirjana and Jovanović, Marina and Ninković, Milica and Stevanović, Ivana and Đurđević, Dragan and Vasić, Una",
year = "2012",
abstract = "In this study we examined if the response of the cortex against diquat (DQ), intrastriatally (i.s.) applied to Wistar rats, was mediated by oxidative/nitrosative stress (OS/NS). In particular, we were focused on the glutathione (GSH) antioxidative role, thus we applied i.s. glutathione reductase (GR) in the pre-treatment of DQ administration. Superoxide anion radical (O2 •-), nitrate (NO3 -), malondialdehyde (MDA) and superoxide dismutase (SOD), were measured in ipsi- and contra- lateral sides of the cortex, at 30 minutes, 24 hours and 7 days post treatment. The redox balance was not significantly changed in the cortex of sham operated and intact groups. Also, no differences were observed between the ipsi- and contra- lateral side of the cortex. Lethargy and mortality (30-40%) of the animals in the DQ group within 24 hrs, coincided with rapidly developed lipid peroxidation supported by OS/NS upon i.s. DQ administration. Strong redox potential of DQ probably resulted in a huge deprivation of molecular oxygen. The pretreatment with GR acted neuro-protectively, based on animal survival and absence of lethargy, although, lipid peroxidation was not developed in the GR+DQ group, OS was documented by a high concentration of O2 •- (within 24 hrs), descending and eventually inhibiting SOD activity (at 7 days)., U ovoj studiji smo ispitali da li je oksidativni/nitrosativni stres (OS/NS), uključen u odgovor korteksa Wistar pacova nakon intrastrijatalne (i.s.) izloženosti dikvatu (DK). Posebno smo ispitivali značaj antioksidativne uloge glutationa (GSH), zbog čega smo primenili glutation reduktazu (GR) u predtretmanu davanja DK. Superoksid anjon radikal (O2•¯), nitrati (NO3¯), malondialdehid (MDA) i superoksid dismutaza (SOD), su mereni u obostranom korteksu (ipsi- i kontrastrana), nakon 30 minuta, 24 sati i 7 dana od tretmana. Redoks balans se nije značajno promenio u korteksu lažno operisanih i netretiranih pacova. Takođe, ne postoji statistički značajna razlika između ipsi- i kontra- strane korteksa. Letargija i mortalitet (30-40%) kod životinja u DK grupi su uočene tokom 24 časa od i.s. trovanja DK, što se poklopilo sa naglim razvojem OS/NS i lipidne peroksidacije. Visok redoks potencijal DK verovatno rezultira opsežnim utroškom molekularnog kiseonika. Zaključeno je da je ostvaren neuroprotektivni učinak predtretmana sa GR, na osnovu preživljavanja životinja i odsustva letargije. Lipidna peroksidacija nije bila razvijena u grupi predtretiranoj sa GR ali je ipak izmerena visoka koncentracija O2•¯ (tokom 24 sata) koja zatim opada i na kraju 7. dana u potpunosti inhibira aktivnost SOD.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta veterinaria",
title = "Influence of glutathione reductase on diquat neurotoxcity assessed by oxidative/nitrosative stress in the cortex of intrastriatally treated rats, Uticaj glutation reduktaze na neurotoksičnost dikvata - ispitivan je oksidativni/nitrozativni stres u korteksu intrastrijatalno tretiranih pacova",
volume = "62",
number = "5-6",
pages = "553-568",
doi = "10.2298/AVB1206553D"
}

Đukić, M., Jovanović, M., Ninković, M., Stevanović, I., Đurđević, D.,& Vasić, U.. (2012). Influence of glutathione reductase on diquat neurotoxcity assessed by oxidative/nitrosative stress in the cortex of intrastriatally treated rats. in Acta veterinaria
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 62(5-6), 553-568.
https://doi.org/10.2298/AVB1206553D

Đukić M, Jovanović M, Ninković M, Stevanović I, Đurđević D, Vasić U. Influence of glutathione reductase on diquat neurotoxcity assessed by oxidative/nitrosative stress in the cortex of intrastriatally treated rats. in Acta veterinaria. 2012;62(5-6):553-568.
doi:10.2298/AVB1206553D .

Đukić, Mirjana, Jovanović, Marina, Ninković, Milica, Stevanović, Ivana, Đurđević, Dragan, Vasić, Una, "Influence of glutathione reductase on diquat neurotoxcity assessed by oxidative/nitrosative stress in the cortex of intrastriatally treated rats" in Acta veterinaria, 62, no. 5-6 (2012):553-568,
https://doi.org/10.2298/AVB1206553D . .

TY  - JOUR
AU  - Đukić, Mirjana
AU  - Ninković, Milica
AU  - Stevanović, Ivana
AU  - Ćurčić, Marijana
AU  - Ilić, Katarina
AU  - Đurđević, Dragan
AU  - Vujanović, Dragana
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1781
AB  - Most commonly observed central nervous system (CNS) effects induced by systemic toxicity of herbicide diquat (DQ) are general depression and lethargy. Generally, it is accepted that DQ exerts its toxicity through the production of superoxide anion radical (O2 ●-) during its redox metabolism in the presence of molecular oxygen, which further initiates radical chain reaction, contributing developing of oxidative stress (OS) as well. Mechanisms of DQ neurotoxic effect is not rationalized till now. The objective of the study was to examine whether OS contributes to DQ neurotxicity. For this purpose, male Wistar rats were intrastriataly (i.s.) treated with DQ and oxidative status parameters such as: superoxide anion radical (O2 ●-); nitrates (NO3 -), as a final metabolite of reactive nitogen species; malondialdehyde (MDA), an indicator of lipid peroxidation; activity of superoxide dismutase (SOD); glutathione peroxidase (GPx), and glutathione (GSH), were measured in the hippocampus at 30 minutes, 24 hours and 7 days post treatment. Noteworthy, mortality rate (30 - 40 %) was observed in the group of rats treated with DQ, within 2-3 hours after awakening from anesthesia. Additionally, lethargy was the only neurological symptom observed in that group. Analyzed parameters indicate that OS mediates DQ neurotxicity, which is documented with significant increase of lipid peroxidation.
AB  - Najčešće zapaženi efekti sistemskog trovanja herbicidom dikvatom (DK) na centralni nervni sistem (CNS) su opšta depresija i letargija. Opšte je prihvaćeno da se toksičnost DK ostvaruje posredstvom povećanog stvaranja superoksid anjon radikala (O2 ●-) tokom njegovog redoks metabolizma, u prisustvu molekularnog kiseonika, koji dalje inicira lančanu reakciju radikalskog tipa i razvoj oksidativnog stresa (OS). Do danas mehanizmi neurotoksičnog efekta DK nisu u potpunosti poznati. Cilj ove studije je bio da ispitamo da li OS posreduje u neurotoksičnosti indukovanoj DK. Eksperiment je sproveden na mužjacima Wistar pacova, intrastrijatalno tretiranih (i.s.) DK. Parametri oksidativnog statusa, kao što su: superoksid anjon radikal (O2 ●-), nitrati (NO3 -), kao finalni metaboliti reaktivnih vrsta azota; malondialdehid (MDA), indikator lipidne peroksidacije; aktivnost enzima; superoksid dizmutaze (SOD) i glutation peroksidaze (GPx); i glutation (GSH) mereni su u hipokampusu, 30 minuta, 24 sati i 7 dana posle tretmana. Stopa smrtnosti od 30 do 40 % ustanovljena je u grupi pacova tretiranih DK, tokom 2-3 sata od buđenja iz anestezije. Dodatno, pacovi ove grupe su pokazali neurološke simptome letargije. Značajno povećana lipidna peroksidacija pokazuju da OS posreduje u neurotoksičnom odgovoru indukovanom i.s. primenom DK.
PB  - Savez farmaceutskih udruženja Srbije, Beograd
T2  - Arhiv za farmaciju
T1  - Oxidative and nitrosative stress: Mediators of diquat neurotoxicity
T1  - Oksidativni i nitrozativni stres - medijatori neurotoksičnosti dikvata
VL  - 62
IS  - 5
SP  - 443
EP  - 460
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1781
ER  - 

@article{
author = "Đukić, Mirjana and Ninković, Milica and Stevanović, Ivana and Ćurčić, Marijana and Ilić, Katarina and Đurđević, Dragan and Vujanović, Dragana",
year = "2012",
abstract = "Most commonly observed central nervous system (CNS) effects induced by systemic toxicity of herbicide diquat (DQ) are general depression and lethargy. Generally, it is accepted that DQ exerts its toxicity through the production of superoxide anion radical (O2 ●-) during its redox metabolism in the presence of molecular oxygen, which further initiates radical chain reaction, contributing developing of oxidative stress (OS) as well. Mechanisms of DQ neurotoxic effect is not rationalized till now. The objective of the study was to examine whether OS contributes to DQ neurotxicity. For this purpose, male Wistar rats were intrastriataly (i.s.) treated with DQ and oxidative status parameters such as: superoxide anion radical (O2 ●-); nitrates (NO3 -), as a final metabolite of reactive nitogen species; malondialdehyde (MDA), an indicator of lipid peroxidation; activity of superoxide dismutase (SOD); glutathione peroxidase (GPx), and glutathione (GSH), were measured in the hippocampus at 30 minutes, 24 hours and 7 days post treatment. Noteworthy, mortality rate (30 - 40 %) was observed in the group of rats treated with DQ, within 2-3 hours after awakening from anesthesia. Additionally, lethargy was the only neurological symptom observed in that group. Analyzed parameters indicate that OS mediates DQ neurotxicity, which is documented with significant increase of lipid peroxidation., Najčešće zapaženi efekti sistemskog trovanja herbicidom dikvatom (DK) na centralni nervni sistem (CNS) su opšta depresija i letargija. Opšte je prihvaćeno da se toksičnost DK ostvaruje posredstvom povećanog stvaranja superoksid anjon radikala (O2 ●-) tokom njegovog redoks metabolizma, u prisustvu molekularnog kiseonika, koji dalje inicira lančanu reakciju radikalskog tipa i razvoj oksidativnog stresa (OS). Do danas mehanizmi neurotoksičnog efekta DK nisu u potpunosti poznati. Cilj ove studije je bio da ispitamo da li OS posreduje u neurotoksičnosti indukovanoj DK. Eksperiment je sproveden na mužjacima Wistar pacova, intrastrijatalno tretiranih (i.s.) DK. Parametri oksidativnog statusa, kao što su: superoksid anjon radikal (O2 ●-), nitrati (NO3 -), kao finalni metaboliti reaktivnih vrsta azota; malondialdehid (MDA), indikator lipidne peroksidacije; aktivnost enzima; superoksid dizmutaze (SOD) i glutation peroksidaze (GPx); i glutation (GSH) mereni su u hipokampusu, 30 minuta, 24 sati i 7 dana posle tretmana. Stopa smrtnosti od 30 do 40 % ustanovljena je u grupi pacova tretiranih DK, tokom 2-3 sata od buđenja iz anestezije. Dodatno, pacovi ove grupe su pokazali neurološke simptome letargije. Značajno povećana lipidna peroksidacija pokazuju da OS posreduje u neurotoksičnom odgovoru indukovanom i.s. primenom DK.",
publisher = "Savez farmaceutskih udruženja Srbije, Beograd",
journal = "Arhiv za farmaciju",
title = "Oxidative and nitrosative stress: Mediators of diquat neurotoxicity, Oksidativni i nitrozativni stres - medijatori neurotoksičnosti dikvata",
volume = "62",
number = "5",
pages = "443-460",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1781"
}

Đukić, M., Ninković, M., Stevanović, I., Ćurčić, M., Ilić, K., Đurđević, D.,& Vujanović, D.. (2012). Oxidative and nitrosative stress: Mediators of diquat neurotoxicity. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije, Beograd., 62(5), 443-460.
https://hdl.handle.net/21.15107/rcub_farfar_1781

Đukić M, Ninković M, Stevanović I, Ćurčić M, Ilić K, Đurđević D, Vujanović D. Oxidative and nitrosative stress: Mediators of diquat neurotoxicity. in Arhiv za farmaciju. 2012;62(5):443-460.
https://hdl.handle.net/21.15107/rcub_farfar_1781 .

Đukić, Mirjana, Ninković, Milica, Stevanović, Ivana, Ćurčić, Marijana, Ilić, Katarina, Đurđević, Dragan, Vujanović, Dragana, "Oxidative and nitrosative stress: Mediators of diquat neurotoxicity" in Arhiv za farmaciju, 62, no. 5 (2012):443-460,
https://hdl.handle.net/21.15107/rcub_farfar_1781 .

TY  - JOUR
AU  - Đukić, Mirjana
AU  - Ninković, Milica
AU  - Stevanović, Ivana
AU  - Ilić, Katarina
AU  - Đurđević, Dragan
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1778
AB  - Contact herbicide paraquat (DQ) is bipyridylium compound, which undergoes redox metabolism; hence enlarged in humans radical production mediated its toxicity. Target organs of systemic effect of PQ poisoning are lung and kidney. The mechanism of PQ-induced neurotoxicity is not elucidated till now. The objective of our study was to examine the role of nitric oxide (NOx) in PQ-induced neurotoxicity, primarily focusing on glutathione cycles [total glutathione content (GSH) and glutathione peroxidase (GPx) activity]. In order to investigate the role of NOx in oxidative stress (OS) and/or nitrosative stress (NS) response to PQ neurotoxicity, we used NG-nitro-L-arginine methyl ester (L-NAME), a non-selective inhibitor of nitric oxide synthase (NOS) in the pretreatment of PQ administration. Study was conducted on Wistar rats randomly divided in groups (n=8 for controls and n=24 for experimental groups) depending on the applied treatments. The tested compounds were intrastriatally (i.s.) administered. Measuring of GSH content and GPx activity was performed at 30 min, 24 hours and 7 days after treatments. Parkinsonism’s like symptoms were observed only in the group of rats treated with PQ. The L-NAME protected animals from PQ-induced neurotoxicity, which could be concluded from the absence of Parkinsonism’s like symptoms and reduced OS/NS response in the striatum of rats pretreated with L-NAME.
AB  - Kontaktni herbicid parakvat (PK) je dipiridinsko jedinjenje, koje podleže redoks metabolizmu i svoju toksičnost ispoljava posredstvom povećanog stvaranja slobodnih radikala. Ciljni organi sistemskog toksičnog efekta PK kod čoveka su pluća i bubrezi. Mehanizam PKindukovane neurotoksičnosti do sada još uvek nije u potpunosti rasvetljen. Cilj našeg rada bio je da se ispita uloga azot oksida (NOx) u neurotoksičnosti PK, sa posebnim osvrtom na glutationski ciklus [glutation (GSH) i enzim glutation peroksidazu (GPx)]. U cilju da se istraži uloga NOx u oksidativnom stresu (OS) i / ili nitrosativnom stresu (NS), kao odgovoru na neurotoksičnost PK. U predtretmanu parakvatom koristili smo NG-nitro- L-arginin metil estar (L-NAME), neselektivni inhibitor azot oksid sintetaze (NOS), aplikovan je pre davanja PQ . Studija je sprovedena na pacovima Wistar soja nasumice podeljenim u grupe (n = 8 za kontrolnu grupu i n = 24 za eksperimentalne grupe) u zavisnosti od tretmana. Testirana jedinjenja su intrastrijatalno (i.s.) aplikovana. Merenje sadržaja GSH i aktivnosti GPx izvršena su 30 min, 24 sata i 7 dana posle tretmana. Parkinsonizam je kao simptom primećen samo u grupi pacova tretiranih PK - om. L-NAME je ispoljio zaštitni efekat kod životinja kod kojih je i.s. davan PK, što bi se moglo zaključiti na osnovu odsustva simptoma parkinsonizma i smanjenog OS/NS odgovora u strijatumu u grupi pretretiranoj sa L-NAME.
PB  - Savez farmaceutskih udruženja Srbije, Beograd
T2  - Arhiv za farmaciju
T1  - The effect of pre-treatment with l-name on glutathione and glutathione peroxidase in parquat-induced neurotoxicity in rat striatum
T1  - Efekat predtretmana sa l-name na glutation i glutation peroksidazu u strijatumu pacova na neurotoksičnost izazvanu parakvatom
VL  - 62
IS  - 3
SP  - 237
EP  - 251
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1778
ER  - 

@article{
author = "Đukić, Mirjana and Ninković, Milica and Stevanović, Ivana and Ilić, Katarina and Đurđević, Dragan",
year = "2012",
abstract = "Contact herbicide paraquat (DQ) is bipyridylium compound, which undergoes redox metabolism; hence enlarged in humans radical production mediated its toxicity. Target organs of systemic effect of PQ poisoning are lung and kidney. The mechanism of PQ-induced neurotoxicity is not elucidated till now. The objective of our study was to examine the role of nitric oxide (NOx) in PQ-induced neurotoxicity, primarily focusing on glutathione cycles [total glutathione content (GSH) and glutathione peroxidase (GPx) activity]. In order to investigate the role of NOx in oxidative stress (OS) and/or nitrosative stress (NS) response to PQ neurotoxicity, we used NG-nitro-L-arginine methyl ester (L-NAME), a non-selective inhibitor of nitric oxide synthase (NOS) in the pretreatment of PQ administration. Study was conducted on Wistar rats randomly divided in groups (n=8 for controls and n=24 for experimental groups) depending on the applied treatments. The tested compounds were intrastriatally (i.s.) administered. Measuring of GSH content and GPx activity was performed at 30 min, 24 hours and 7 days after treatments. Parkinsonism’s like symptoms were observed only in the group of rats treated with PQ. The L-NAME protected animals from PQ-induced neurotoxicity, which could be concluded from the absence of Parkinsonism’s like symptoms and reduced OS/NS response in the striatum of rats pretreated with L-NAME., Kontaktni herbicid parakvat (PK) je dipiridinsko jedinjenje, koje podleže redoks metabolizmu i svoju toksičnost ispoljava posredstvom povećanog stvaranja slobodnih radikala. Ciljni organi sistemskog toksičnog efekta PK kod čoveka su pluća i bubrezi. Mehanizam PKindukovane neurotoksičnosti do sada još uvek nije u potpunosti rasvetljen. Cilj našeg rada bio je da se ispita uloga azot oksida (NOx) u neurotoksičnosti PK, sa posebnim osvrtom na glutationski ciklus [glutation (GSH) i enzim glutation peroksidazu (GPx)]. U cilju da se istraži uloga NOx u oksidativnom stresu (OS) i / ili nitrosativnom stresu (NS), kao odgovoru na neurotoksičnost PK. U predtretmanu parakvatom koristili smo NG-nitro- L-arginin metil estar (L-NAME), neselektivni inhibitor azot oksid sintetaze (NOS), aplikovan je pre davanja PQ . Studija je sprovedena na pacovima Wistar soja nasumice podeljenim u grupe (n = 8 za kontrolnu grupu i n = 24 za eksperimentalne grupe) u zavisnosti od tretmana. Testirana jedinjenja su intrastrijatalno (i.s.) aplikovana. Merenje sadržaja GSH i aktivnosti GPx izvršena su 30 min, 24 sata i 7 dana posle tretmana. Parkinsonizam je kao simptom primećen samo u grupi pacova tretiranih PK - om. L-NAME je ispoljio zaštitni efekat kod životinja kod kojih je i.s. davan PK, što bi se moglo zaključiti na osnovu odsustva simptoma parkinsonizma i smanjenog OS/NS odgovora u strijatumu u grupi pretretiranoj sa L-NAME.",
publisher = "Savez farmaceutskih udruženja Srbije, Beograd",
journal = "Arhiv za farmaciju",
title = "The effect of pre-treatment with l-name on glutathione and glutathione peroxidase in parquat-induced neurotoxicity in rat striatum, Efekat predtretmana sa l-name na glutation i glutation peroksidazu u strijatumu pacova na neurotoksičnost izazvanu parakvatom",
volume = "62",
number = "3",
pages = "237-251",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1778"
}

Đukić, M., Ninković, M., Stevanović, I., Ilić, K.,& Đurđević, D.. (2012). The effect of pre-treatment with l-name on glutathione and glutathione peroxidase in parquat-induced neurotoxicity in rat striatum. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije, Beograd., 62(3), 237-251.
https://hdl.handle.net/21.15107/rcub_farfar_1778

Đukić M, Ninković M, Stevanović I, Ilić K, Đurđević D. The effect of pre-treatment with l-name on glutathione and glutathione peroxidase in parquat-induced neurotoxicity in rat striatum. in Arhiv za farmaciju. 2012;62(3):237-251.
https://hdl.handle.net/21.15107/rcub_farfar_1778 .

Đukić, Mirjana, Ninković, Milica, Stevanović, Ivana, Ilić, Katarina, Đurđević, Dragan, "The effect of pre-treatment with l-name on glutathione and glutathione peroxidase in parquat-induced neurotoxicity in rat striatum" in Arhiv za farmaciju, 62, no. 3 (2012):237-251,
https://hdl.handle.net/21.15107/rcub_farfar_1778 .

TY  - JOUR
AU  - Đukić, Mirjana
AU  - Jovanović, Marina
AU  - Ninković, Milica
AU  - Stevanović, Ivana
AU  - Ilić, Katarina
AU  - Ćurčić, Marijana
AU  - Vekić, Jelena
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1653
AB  - Paraquat (PQ), a widely used herbicide is a well-known free radical producing agent. The mechanistic pathways of PQ neurotoxicity were examined by assessing oxidative/nitrosative stress markers. Focus was on the role of glutathione (GSH) cycle and to examine whether the pre-treatment with enzyme glutathione reductase (GR) could protect the vulnerable brain regions (VBRs) against harmful oxidative effect of PQ. The study was conducted on Wistar rats, randomly divided in five groups: intact-control group, (n = 8) and four experimental groups (n = 24). All tested compounds were administered intrastriatally (i.s.) in one single dose. The following parameters of oxidative status were measured in the striatum, hippocampus and cortex, at 30 min, 24 h and 7 days post treatment: superoxide anion radical (O-2(center dot-)), nitrate (NO3-). malondialdehyde (MDA), superoxide dismutase (SOD), total GSH (tGSH) and its oxidized, disulfide form (GSSG) and glutathione peroxidase (GPx). Results obtained from the intact and the sham operated groups were not statistically different, confirming that invasive i.s. route of administration would not influence the reliability of results. Also, similar pattern of changes were observed between ipsi- and contra- lateral side of examined VBRs, indicating rapid spatial spreading of oxidative stress. Mortality of the animals (10%), within 24 h, along with symptoms of Parkinsonism, after awakening from anesthesia for 2-3 h, were observed in the PQ group, only. Increased levels of O2(center dot-), NO3- and MDA, increased ratio of GSSG/GSH and considerably high activity of GPx were measured at 30 min after the treatment. Cytotoxic effect of PQ was documented by drastic drop of all measured parameters and extremely high peak of the ratio GSSG/GSH at 24th hrs after the PQ i.s. injection. In the GR + PQ group, markedly low activity of GPx and low content of NO3- (in striatum and cortex) were measured during whole experiment, while increase value was observed only for O-2(center dot-), at 7th days. We concluded that oxidative/nitrosative stress and excitotoxicity are the most important events since the early stage of PQ induced neurotoxicity. Based on the ratio GSSG/GSH, the oxidation of GSH to GSSG is probably dominant way of GHS depletion and main reason for reduced antioxidative defense against PQ harmful oxidative effect. The GR pre-treatment resulted in the absence of Parkinson's disease-like symptoms and mortality of the rats. Additionally, oxidative/nitrosative stress did not developed, as well as almost diminished metabolism of the VBRs at 24th hours (as has been documented in the PQgroup) did not occurred in the GR + PQ suggesting a neuroprotective role for the GR in PQ induced neurotoxicity.
PB  - Elsevier Ireland Ltd, Clare
T2  - Chemico-Biological Interactions
T1  - Protective role of glutathione reductase in paraquat induced neurotoxicity
VL  - 199
IS  - 2
SP  - 74
EP  - 86
DO  - 10.1016/j.cbi.2012.05.008
ER  - 

@article{
author = "Đukić, Mirjana and Jovanović, Marina and Ninković, Milica and Stevanović, Ivana and Ilić, Katarina and Ćurčić, Marijana and Vekić, Jelena",
year = "2012",
abstract = "Paraquat (PQ), a widely used herbicide is a well-known free radical producing agent. The mechanistic pathways of PQ neurotoxicity were examined by assessing oxidative/nitrosative stress markers. Focus was on the role of glutathione (GSH) cycle and to examine whether the pre-treatment with enzyme glutathione reductase (GR) could protect the vulnerable brain regions (VBRs) against harmful oxidative effect of PQ. The study was conducted on Wistar rats, randomly divided in five groups: intact-control group, (n = 8) and four experimental groups (n = 24). All tested compounds were administered intrastriatally (i.s.) in one single dose. The following parameters of oxidative status were measured in the striatum, hippocampus and cortex, at 30 min, 24 h and 7 days post treatment: superoxide anion radical (O-2(center dot-)), nitrate (NO3-). malondialdehyde (MDA), superoxide dismutase (SOD), total GSH (tGSH) and its oxidized, disulfide form (GSSG) and glutathione peroxidase (GPx). Results obtained from the intact and the sham operated groups were not statistically different, confirming that invasive i.s. route of administration would not influence the reliability of results. Also, similar pattern of changes were observed between ipsi- and contra- lateral side of examined VBRs, indicating rapid spatial spreading of oxidative stress. Mortality of the animals (10%), within 24 h, along with symptoms of Parkinsonism, after awakening from anesthesia for 2-3 h, were observed in the PQ group, only. Increased levels of O2(center dot-), NO3- and MDA, increased ratio of GSSG/GSH and considerably high activity of GPx were measured at 30 min after the treatment. Cytotoxic effect of PQ was documented by drastic drop of all measured parameters and extremely high peak of the ratio GSSG/GSH at 24th hrs after the PQ i.s. injection. In the GR + PQ group, markedly low activity of GPx and low content of NO3- (in striatum and cortex) were measured during whole experiment, while increase value was observed only for O-2(center dot-), at 7th days. We concluded that oxidative/nitrosative stress and excitotoxicity are the most important events since the early stage of PQ induced neurotoxicity. Based on the ratio GSSG/GSH, the oxidation of GSH to GSSG is probably dominant way of GHS depletion and main reason for reduced antioxidative defense against PQ harmful oxidative effect. The GR pre-treatment resulted in the absence of Parkinson's disease-like symptoms and mortality of the rats. Additionally, oxidative/nitrosative stress did not developed, as well as almost diminished metabolism of the VBRs at 24th hours (as has been documented in the PQgroup) did not occurred in the GR + PQ suggesting a neuroprotective role for the GR in PQ induced neurotoxicity.",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Chemico-Biological Interactions",
title = "Protective role of glutathione reductase in paraquat induced neurotoxicity",
volume = "199",
number = "2",
pages = "74-86",
doi = "10.1016/j.cbi.2012.05.008"
}

Đukić, M., Jovanović, M., Ninković, M., Stevanović, I., Ilić, K., Ćurčić, M.,& Vekić, J.. (2012). Protective role of glutathione reductase in paraquat induced neurotoxicity. in Chemico-Biological Interactions
Elsevier Ireland Ltd, Clare., 199(2), 74-86.
https://doi.org/10.1016/j.cbi.2012.05.008

Đukić M, Jovanović M, Ninković M, Stevanović I, Ilić K, Ćurčić M, Vekić J. Protective role of glutathione reductase in paraquat induced neurotoxicity. in Chemico-Biological Interactions. 2012;199(2):74-86.
doi:10.1016/j.cbi.2012.05.008 .

Đukić, Mirjana, Jovanović, Marina, Ninković, Milica, Stevanović, Ivana, Ilić, Katarina, Ćurčić, Marijana, Vekić, Jelena, "Protective role of glutathione reductase in paraquat induced neurotoxicity" in Chemico-Biological Interactions, 199, no. 2 (2012):74-86,
https://doi.org/10.1016/j.cbi.2012.05.008 . .

TY  - JOUR
AU  - Ninković, Milica
AU  - Maliević, Ivorad M.
AU  - Jelenković, Ankica V.
AU  - Đukić, Mirjana
AU  - Jovanović, Marina
AU  - Stevanović, Ivana
PY  - 2009
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1169
AB  - Oxidative stress development in different brain structures and the influence of nitric oxide (NO) overproduction during sepsis was investigated using male Wistar rats. Rats were subjected to cecal ligation and puncture (CLP) or were sham-operated. To evaluate the source of NO production, 30 min before the operation septic and control animals were treated with single intraperitoneal doses of NO synthase (NOS) inhibitors: L-NAME and aminoguanidine (AG) (10, 30 or 90 mg/kg) and 7-nitroindazole (7-NI) (30 mg/kg). The concentration of GSH in the cortex, brain stem, cerebellum, striatum and hippocampus significantly decreased post CLP at both early and late stage sepsis. Lipid peroxidation also occurred in the cortex and cerebellum in late stage sepsis. Pre-treatment with a non-selective NOS inhibitor (L-NAME) and with selective inducible and neuronal NOS inhibitors (AG and 7-NI) revealed benefit effects on the GSH concentrations. Unexpectedly, NOS inhibition resulted in diverse effects on TBARS concentrations, suggesting the importance of specific quantities of NO in specific brain structures during sepsis.
PB  - General Physiol And Biophysics, Bratislava
T2  - General Physiology and Biophysics
T1  - Nitric oxide synthase inhibitors partially inhibit oxidative stress development in the rat brain during sepsis provoked by cecal ligation and puncture
VL  - 28
SP  - 243
EP  - 250
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_1476
ER  - 

@article{
author = "Ninković, Milica and Maliević, Ivorad M. and Jelenković, Ankica V. and Đukić, Mirjana and Jovanović, Marina and Stevanović, Ivana",
year = "2009",
abstract = "Oxidative stress development in different brain structures and the influence of nitric oxide (NO) overproduction during sepsis was investigated using male Wistar rats. Rats were subjected to cecal ligation and puncture (CLP) or were sham-operated. To evaluate the source of NO production, 30 min before the operation septic and control animals were treated with single intraperitoneal doses of NO synthase (NOS) inhibitors: L-NAME and aminoguanidine (AG) (10, 30 or 90 mg/kg) and 7-nitroindazole (7-NI) (30 mg/kg). The concentration of GSH in the cortex, brain stem, cerebellum, striatum and hippocampus significantly decreased post CLP at both early and late stage sepsis. Lipid peroxidation also occurred in the cortex and cerebellum in late stage sepsis. Pre-treatment with a non-selective NOS inhibitor (L-NAME) and with selective inducible and neuronal NOS inhibitors (AG and 7-NI) revealed benefit effects on the GSH concentrations. Unexpectedly, NOS inhibition resulted in diverse effects on TBARS concentrations, suggesting the importance of specific quantities of NO in specific brain structures during sepsis.",
publisher = "General Physiol And Biophysics, Bratislava",
journal = "General Physiology and Biophysics",
title = "Nitric oxide synthase inhibitors partially inhibit oxidative stress development in the rat brain during sepsis provoked by cecal ligation and puncture",
volume = "28",
pages = "243-250",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_1476"
}

Ninković, M., Maliević, I. M., Jelenković, A. V., Đukić, M., Jovanović, M.,& Stevanović, I.. (2009). Nitric oxide synthase inhibitors partially inhibit oxidative stress development in the rat brain during sepsis provoked by cecal ligation and puncture. in General Physiology and Biophysics
General Physiol And Biophysics, Bratislava., 28, 243-250.
https://hdl.handle.net/21.15107/rcub_ibiss_1476

Ninković M, Maliević IM, Jelenković AV, Đukić M, Jovanović M, Stevanović I. Nitric oxide synthase inhibitors partially inhibit oxidative stress development in the rat brain during sepsis provoked by cecal ligation and puncture. in General Physiology and Biophysics. 2009;28:243-250.
https://hdl.handle.net/21.15107/rcub_ibiss_1476 .

Ninković, Milica, Maliević, Ivorad M., Jelenković, Ankica V., Đukić, Mirjana, Jovanović, Marina, Stevanović, Ivana, "Nitric oxide synthase inhibitors partially inhibit oxidative stress development in the rat brain during sepsis provoked by cecal ligation and puncture" in General Physiology and Biophysics, 28 (2009):243-250,
https://hdl.handle.net/21.15107/rcub_ibiss_1476 .