TY  - JOUR
AU  - Ušjak, Ljuboš
AU  - Drobac, Milica
AU  - Ivanov, Marija
AU  - Soković, Marina
AU  - Milenković, Marina
AU  - Niketić, Marjan
AU  - Petrović, Silvana
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4469
AB  - Composition and antimicrobial activity of root, leaf, stem, flower and fruit essential oils from
cultivated Pastinaca sativa subsp. sativa, and its two wild-growing relatives P. sativa subsp. urens
and P. hirsuta (Apiaceae) were investigated. Twenty-nine hydrodistilled essential oils of plants from
different localities and/or years were analysed by GC-FID and GC/MS. Dominant in root oils was
myristicin (P. sativa) or apiole (P. hirsuta), in leaf and stem oils myristicin (cultivated plants) or γ-palmitolactone (wild-growing plants) and in flower and fruit oils aliphatic esters. Multivariate
statistics (PCA, nMDS, UPGMA clustering) generally revealed separation of oils of investigated
Pastinaca taxa and demonstrated their chemosystematic significance. One oil per each organ of all three plants (fifteen in total) was tested using microdilution method for activity against Candida
tropicalis, C. parapsilosis, C. krusei, C. glabrata, C. albicans, Staphylococcus aureus, Bacillus cereus, Listeria monocytogenes, Escherichia coli, Salmonella Typhimurium and Enterobacter cloacae; MIC = 0.25–8 mg/mL, MBC(MFC) = 0.5–16 mg/mL.
PB  - Taylor and Francis Group
T2  - Journal of Essential Oil Research
T1  - Composition and antimicrobial activity of Pastinaca sativa subsp. sativa, P. sativa subsp. urens and P. hirsuta essential oils
VL  - 35
IS  - 2
SP  - 154
EP  - 167
DO  - 10.1080/10412905.2022.2147675
ER  - 

@article{
author = "Ušjak, Ljuboš and Drobac, Milica and Ivanov, Marija and Soković, Marina and Milenković, Marina and Niketić, Marjan and Petrović, Silvana",
year = "2023",
abstract = "Composition and antimicrobial activity of root, leaf, stem, flower and fruit essential oils from
cultivated Pastinaca sativa subsp. sativa, and its two wild-growing relatives P. sativa subsp. urens
and P. hirsuta (Apiaceae) were investigated. Twenty-nine hydrodistilled essential oils of plants from
different localities and/or years were analysed by GC-FID and GC/MS. Dominant in root oils was
myristicin (P. sativa) or apiole (P. hirsuta), in leaf and stem oils myristicin (cultivated plants) or γ-palmitolactone (wild-growing plants) and in flower and fruit oils aliphatic esters. Multivariate
statistics (PCA, nMDS, UPGMA clustering) generally revealed separation of oils of investigated
Pastinaca taxa and demonstrated their chemosystematic significance. One oil per each organ of all three plants (fifteen in total) was tested using microdilution method for activity against Candida
tropicalis, C. parapsilosis, C. krusei, C. glabrata, C. albicans, Staphylococcus aureus, Bacillus cereus, Listeria monocytogenes, Escherichia coli, Salmonella Typhimurium and Enterobacter cloacae; MIC = 0.25–8 mg/mL, MBC(MFC) = 0.5–16 mg/mL.",
publisher = "Taylor and Francis Group",
journal = "Journal of Essential Oil Research",
title = "Composition and antimicrobial activity of Pastinaca sativa subsp. sativa, P. sativa subsp. urens and P. hirsuta essential oils",
volume = "35",
number = "2",
pages = "154-167",
doi = "10.1080/10412905.2022.2147675"
}

Ušjak, L., Drobac, M., Ivanov, M., Soković, M., Milenković, M., Niketić, M.,& Petrović, S.. (2023). Composition and antimicrobial activity of Pastinaca sativa subsp. sativa, P. sativa subsp. urens and P. hirsuta essential oils. in Journal of Essential Oil Research
Taylor and Francis Group., 35(2), 154-167.
https://doi.org/10.1080/10412905.2022.2147675

Ušjak L, Drobac M, Ivanov M, Soković M, Milenković M, Niketić M, Petrović S. Composition and antimicrobial activity of Pastinaca sativa subsp. sativa, P. sativa subsp. urens and P. hirsuta essential oils. in Journal of Essential Oil Research. 2023;35(2):154-167.
doi:10.1080/10412905.2022.2147675 .

Ušjak, Ljuboš, Drobac, Milica, Ivanov, Marija, Soković, Marina, Milenković, Marina, Niketić, Marjan, Petrović, Silvana, "Composition and antimicrobial activity of Pastinaca sativa subsp. sativa, P. sativa subsp. urens and P. hirsuta essential oils" in Journal of Essential Oil Research, 35, no. 2 (2023):154-167,
https://doi.org/10.1080/10412905.2022.2147675 . .

TY  - JOUR
AU  - Pavlović, Ivan
AU  - Radenković, Mirjana
AU  - Branković, Suzana
AU  - Milenković, Marina
AU  - Niketić, Marjan
AU  - Ušjak, Ljuboš
AU  - Petrović, Silvana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4084
AB  - Dry MeOH extract of Ferula heuffelii (Apiaceae) underground parts was tested for spasmolytic, gastroprotective and antioxidant activities. HPLC analysis revealed that chlorogenic acid (CGA; 34.6 mg/g) was its main constituent. Extract in vitro exhibited notable total antioxidant activity (FRAP value=1.0 μmol Fe2+/mg), and scavenging of DPPH (SC50=62.5 μg/ml) and •OH radicals (49.5 % at 20 μg/ml in 2-deoxyribose assay). In vitro on isolated rat ileum, extract exhibited significant spasmolytic activity, i. e., it showed 124.6 % of maximal atropine effect on spontaneous contractions (at 100 μg/ml), and reduced spasmogenic effect of KCl (80 mm) to 44.4 % (at 60 μg/ml) and of highest applied concentration of ACh to 26.3 % (at 120 μg/ml). In parallel experiments, spasmolytic effect of CGA was also demonstrated. In acute EtOH-induced gastric ulceration model in rats, extract (100 mg/kg p.o.) showed significant gastroprotective effect (gastric damage score 0.50), similar to ranitidine (20 mg/kg p.o.). Obtained results showed that tested F. heuffelii polar extract represents new herbal preparation with potential use against some gastrointestinal complaints.
PB  - John Wiley and Sons Inc
T2  - Chemistry and Biodiversity
T1  - Spasmolytic, Gastroprotective and Antioxidant Activities of Dry Methanol Extract of Ferula heuffelii Underground Parts
VL  - 19
IS  - 5
DO  - 10.1002/cbdv.202200047
ER  - 

@article{
author = "Pavlović, Ivan and Radenković, Mirjana and Branković, Suzana and Milenković, Marina and Niketić, Marjan and Ušjak, Ljuboš and Petrović, Silvana",
year = "2022",
abstract = "Dry MeOH extract of Ferula heuffelii (Apiaceae) underground parts was tested for spasmolytic, gastroprotective and antioxidant activities. HPLC analysis revealed that chlorogenic acid (CGA; 34.6 mg/g) was its main constituent. Extract in vitro exhibited notable total antioxidant activity (FRAP value=1.0 μmol Fe2+/mg), and scavenging of DPPH (SC50=62.5 μg/ml) and •OH radicals (49.5 % at 20 μg/ml in 2-deoxyribose assay). In vitro on isolated rat ileum, extract exhibited significant spasmolytic activity, i. e., it showed 124.6 % of maximal atropine effect on spontaneous contractions (at 100 μg/ml), and reduced spasmogenic effect of KCl (80 mm) to 44.4 % (at 60 μg/ml) and of highest applied concentration of ACh to 26.3 % (at 120 μg/ml). In parallel experiments, spasmolytic effect of CGA was also demonstrated. In acute EtOH-induced gastric ulceration model in rats, extract (100 mg/kg p.o.) showed significant gastroprotective effect (gastric damage score 0.50), similar to ranitidine (20 mg/kg p.o.). Obtained results showed that tested F. heuffelii polar extract represents new herbal preparation with potential use against some gastrointestinal complaints.",
publisher = "John Wiley and Sons Inc",
journal = "Chemistry and Biodiversity",
title = "Spasmolytic, Gastroprotective and Antioxidant Activities of Dry Methanol Extract of Ferula heuffelii Underground Parts",
volume = "19",
number = "5",
doi = "10.1002/cbdv.202200047"
}

Pavlović, I., Radenković, M., Branković, S., Milenković, M., Niketić, M., Ušjak, L.,& Petrović, S.. (2022). Spasmolytic, Gastroprotective and Antioxidant Activities of Dry Methanol Extract of Ferula heuffelii Underground Parts. in Chemistry and Biodiversity
John Wiley and Sons Inc., 19(5).
https://doi.org/10.1002/cbdv.202200047

Pavlović I, Radenković M, Branković S, Milenković M, Niketić M, Ušjak L, Petrović S. Spasmolytic, Gastroprotective and Antioxidant Activities of Dry Methanol Extract of Ferula heuffelii Underground Parts. in Chemistry and Biodiversity. 2022;19(5).
doi:10.1002/cbdv.202200047 .

Pavlović, Ivan, Radenković, Mirjana, Branković, Suzana, Milenković, Marina, Niketić, Marjan, Ušjak, Ljuboš, Petrović, Silvana, "Spasmolytic, Gastroprotective and Antioxidant Activities of Dry Methanol Extract of Ferula heuffelii Underground Parts" in Chemistry and Biodiversity, 19, no. 5 (2022),
https://doi.org/10.1002/cbdv.202200047 . .

TY  - CONF
AU  - Arsenijević, Jelena
AU  - Slavkovska, Violeta
AU  - Milenković, Marina
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4625
AB  - Introduction
Hyssopus officinalis L. (Lamiaceae) is a perennial aromatic subshrub, distributed mainly in the Mediterranean area. The aerial parts of the plant are used as a culinary and medicinal herb (Hyssopi herba) against various ailments, and its essential oil is used in the perfume industry. It contains essential oil, phenolic acids, and flavonoids. However, in a number of cases, certain differences arose between samples of different origin (Mićović et al., 2019). In this work, we investigated the anatomy and chemical composition of H. officinalis from Mt. Galičica, North Macedonia. Furthermore, based on an a priori knowledge on good antimicrobial activity of essential oils, we investigated its effects against selected microorganisms.
PB  - Macedonian Pharmaceutical Association
PB  - Ss. Cyril and Methodius University in Skopje, Faculty of Pharmacy
C3  - Macedonian Pharmaceutical Bulletin
T1  - Anatomical characterization, chemical analyses and essential oil antimicrobial activity of Hyssopus officinalis L. (Lamiaceae)
VL  - 68
IS  - 1 suppl
SP  - 421
EP  - 422
DO  - 10.33320/maced.pharm.bull.2022.68.03.203
ER  - 

@conference{
author = "Arsenijević, Jelena and Slavkovska, Violeta and Milenković, Marina",
year = "2022",
abstract = "Introduction
Hyssopus officinalis L. (Lamiaceae) is a perennial aromatic subshrub, distributed mainly in the Mediterranean area. The aerial parts of the plant are used as a culinary and medicinal herb (Hyssopi herba) against various ailments, and its essential oil is used in the perfume industry. It contains essential oil, phenolic acids, and flavonoids. However, in a number of cases, certain differences arose between samples of different origin (Mićović et al., 2019). In this work, we investigated the anatomy and chemical composition of H. officinalis from Mt. Galičica, North Macedonia. Furthermore, based on an a priori knowledge on good antimicrobial activity of essential oils, we investigated its effects against selected microorganisms.",
publisher = "Macedonian Pharmaceutical Association, Ss. Cyril and Methodius University in Skopje, Faculty of Pharmacy",
journal = "Macedonian Pharmaceutical Bulletin",
title = "Anatomical characterization, chemical analyses and essential oil antimicrobial activity of Hyssopus officinalis L. (Lamiaceae)",
volume = "68",
number = "1 suppl",
pages = "421-422",
doi = "10.33320/maced.pharm.bull.2022.68.03.203"
}

Arsenijević, J., Slavkovska, V.,& Milenković, M.. (2022). Anatomical characterization, chemical analyses and essential oil antimicrobial activity of Hyssopus officinalis L. (Lamiaceae). in Macedonian Pharmaceutical Bulletin
Macedonian Pharmaceutical Association., 68(1 suppl), 421-422.
https://doi.org/10.33320/maced.pharm.bull.2022.68.03.203

Arsenijević J, Slavkovska V, Milenković M. Anatomical characterization, chemical analyses and essential oil antimicrobial activity of Hyssopus officinalis L. (Lamiaceae). in Macedonian Pharmaceutical Bulletin. 2022;68(1 suppl):421-422.
doi:10.33320/maced.pharm.bull.2022.68.03.203 .

Arsenijević, Jelena, Slavkovska, Violeta, Milenković, Marina, "Anatomical characterization, chemical analyses and essential oil antimicrobial activity of Hyssopus officinalis L. (Lamiaceae)" in Macedonian Pharmaceutical Bulletin, 68, no. 1 suppl (2022):421-422,
https://doi.org/10.33320/maced.pharm.bull.2022.68.03.203 . .

TY  - JOUR
AU  - Stojanović, Danilo
AU  - Marčetić, Mirjana
AU  - Ušjak, Dušan
AU  - Milenković, Marina
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4061
AB  - Background/Aim. Plant essential oils (EOs) can have a significant antibacterial effect especially through additive or synergistic action as antibiotic adjuvants. We investi-gated the composition and activity of EOs of two species of genus Salvia (S) from Greece with the aim to deter-mine their antimicrobial activity as well as the activity in combination with selected antibiotics. Methods. The aerial parts of wild-growing S. fruticosa and S. ringens were subjected to a steam distillation and the obtained EOs were analyzed by gas chromatography and gas chroma-tography/mass spectrometry. The broth-microdilution method was used in order to determine the minimum in-hibitory concentrations (MICs) of EOs on seven strains of bacteria and one yeast. Antimicrobial activity of the combination of EO and antibiotics was investigated by checkerboard method and estimated by calculating frac-tional inhibitory concentration (FIC) of each component and fractional inhibitory concentration index (FICI). Re-sults. Dominant component of S. fruticosa EO was trans-thujone (54.2%) and for S. ringens EO it was α-pinene (28.1%). The MICs of EOs of both species were in the range from 200 μg/mL to > 500 μg/mL. The strongest antimicrobial effect was achieved against Bacillus subtilis and Candida albicans. According to FICI values, EO of S. fruticosa had additive effect with ciprofloxacin against most of bacterial strains but not with amikacin. Conclu-sion. The essential oils of S. ringens and S. fruticosa showed modest antimicrobial activity. However, the es-sential oil of S. fruticosa showed a promising additive ef-fect in combination with ciprofloxacin.
AB  - Uvod/Cilj. Etarska ulja različitih biljaka mogu imati značajna antibakterijska svojstva, posebno kao adjuvanti antibiotika sa kojima ostvaruju aditivno ili sinergistično dejstvo. Ispitivali smo sastav i aktivnost etarskih ulja dve vrste roda Salvia (S) iz Grčke sa ciljem da odredimo njihovu antimkrobnu aktivnost, kao i dejstvo u kombi-naciji sa odabranim antibioticima. Metode. Nadzemni delovi samoniklih S. fruticosa i S. ringens su destilovani vodenom parom i dobijena etarska ulja su analizirana gasnom hromatografijom i gasnom hromatografijom sa masenom spektrometrijom. Radi određivanja minimalnih inhibitornih koncentracija (MICs) etarskog ulja na sedam sojeva bakterija i na jednoj patogenoj gljivici korišćena je mikrodiluciona metoda. Antimikrobna aktivnost kombi-nacije etarskog ulja i antibiotika ispitana je checkerboard metodom i procenjena je na osnovu frakcione inhibi-torne koncentracije (FIC) svake komponente i indeksa frakcione inhibitorne koncentracije (FICI). Resultati. Dominantna komponenta etarskog ulja S. fruticosa je bio trans-tujon (54,2%), a etarskog ulja S. ringens α-pinen (28,1%). MICs etarskog ulja obe vrste su bile u opsegu od 200 μg/mL do > 500 μg/mL. Najsnažnija anti-mikrobna aktivnost ostvarena je protiv Bacillus subtilis i Candida albicans. Na osnovu FICI vrednosti, etarsko ulje S. fruticosa je sa ciprofloksacinom, ali ne i sa amikacinom imalo aditivni efekat protiv većine bakterijskih sojeva. Zaključak. Etarska ulja S. ringens i S. fruticosa su pokazala skromnu antimikrobnu aktivnost, ali je etarsko ulje S. fru-ticosa u kombinaciji sa ciprofloksacinom ispoljilo značajan aditivni efekat.
PB  - Belgrade : Military Medical Academy, INI
T2  - Vojnosanitetski pregled
T1  - Composition and antimicrobial activity of essential oils of Salvia fruticosa and Salvia ringens (Lamiaceae)
T1  - Sastav i antimikrobna aktivnost etarskih ulja Salvia fruticosa i Salvia ringens (Lamiaceae)
VL  - 79
IS  - 1
SP  - 62
EP  - 68
DO  - 10.2298/VSP200508083S
ER  - 

@article{
author = "Stojanović, Danilo and Marčetić, Mirjana and Ušjak, Dušan and Milenković, Marina",
year = "2022",
abstract = "Background/Aim. Plant essential oils (EOs) can have a significant antibacterial effect especially through additive or synergistic action as antibiotic adjuvants. We investi-gated the composition and activity of EOs of two species of genus Salvia (S) from Greece with the aim to deter-mine their antimicrobial activity as well as the activity in combination with selected antibiotics. Methods. The aerial parts of wild-growing S. fruticosa and S. ringens were subjected to a steam distillation and the obtained EOs were analyzed by gas chromatography and gas chroma-tography/mass spectrometry. The broth-microdilution method was used in order to determine the minimum in-hibitory concentrations (MICs) of EOs on seven strains of bacteria and one yeast. Antimicrobial activity of the combination of EO and antibiotics was investigated by checkerboard method and estimated by calculating frac-tional inhibitory concentration (FIC) of each component and fractional inhibitory concentration index (FICI). Re-sults. Dominant component of S. fruticosa EO was trans-thujone (54.2%) and for S. ringens EO it was α-pinene (28.1%). The MICs of EOs of both species were in the range from 200 μg/mL to > 500 μg/mL. The strongest antimicrobial effect was achieved against Bacillus subtilis and Candida albicans. According to FICI values, EO of S. fruticosa had additive effect with ciprofloxacin against most of bacterial strains but not with amikacin. Conclu-sion. The essential oils of S. ringens and S. fruticosa showed modest antimicrobial activity. However, the es-sential oil of S. fruticosa showed a promising additive ef-fect in combination with ciprofloxacin., Uvod/Cilj. Etarska ulja različitih biljaka mogu imati značajna antibakterijska svojstva, posebno kao adjuvanti antibiotika sa kojima ostvaruju aditivno ili sinergistično dejstvo. Ispitivali smo sastav i aktivnost etarskih ulja dve vrste roda Salvia (S) iz Grčke sa ciljem da odredimo njihovu antimkrobnu aktivnost, kao i dejstvo u kombi-naciji sa odabranim antibioticima. Metode. Nadzemni delovi samoniklih S. fruticosa i S. ringens su destilovani vodenom parom i dobijena etarska ulja su analizirana gasnom hromatografijom i gasnom hromatografijom sa masenom spektrometrijom. Radi određivanja minimalnih inhibitornih koncentracija (MICs) etarskog ulja na sedam sojeva bakterija i na jednoj patogenoj gljivici korišćena je mikrodiluciona metoda. Antimikrobna aktivnost kombi-nacije etarskog ulja i antibiotika ispitana je checkerboard metodom i procenjena je na osnovu frakcione inhibi-torne koncentracije (FIC) svake komponente i indeksa frakcione inhibitorne koncentracije (FICI). Resultati. Dominantna komponenta etarskog ulja S. fruticosa je bio trans-tujon (54,2%), a etarskog ulja S. ringens α-pinen (28,1%). MICs etarskog ulja obe vrste su bile u opsegu od 200 μg/mL do > 500 μg/mL. Najsnažnija anti-mikrobna aktivnost ostvarena je protiv Bacillus subtilis i Candida albicans. Na osnovu FICI vrednosti, etarsko ulje S. fruticosa je sa ciprofloksacinom, ali ne i sa amikacinom imalo aditivni efekat protiv većine bakterijskih sojeva. Zaključak. Etarska ulja S. ringens i S. fruticosa su pokazala skromnu antimikrobnu aktivnost, ali je etarsko ulje S. fru-ticosa u kombinaciji sa ciprofloksacinom ispoljilo značajan aditivni efekat.",
publisher = "Belgrade : Military Medical Academy, INI",
journal = "Vojnosanitetski pregled",
title = "Composition and antimicrobial activity of essential oils of Salvia fruticosa and Salvia ringens (Lamiaceae), Sastav i antimikrobna aktivnost etarskih ulja Salvia fruticosa i Salvia ringens (Lamiaceae)",
volume = "79",
number = "1",
pages = "62-68",
doi = "10.2298/VSP200508083S"
}

Stojanović, D., Marčetić, M., Ušjak, D.,& Milenković, M.. (2022). Composition and antimicrobial activity of essential oils of Salvia fruticosa and Salvia ringens (Lamiaceae). in Vojnosanitetski pregled
Belgrade : Military Medical Academy, INI., 79(1), 62-68.
https://doi.org/10.2298/VSP200508083S

Stojanović D, Marčetić M, Ušjak D, Milenković M. Composition and antimicrobial activity of essential oils of Salvia fruticosa and Salvia ringens (Lamiaceae). in Vojnosanitetski pregled. 2022;79(1):62-68.
doi:10.2298/VSP200508083S .

Stojanović, Danilo, Marčetić, Mirjana, Ušjak, Dušan, Milenković, Marina, "Composition and antimicrobial activity of essential oils of Salvia fruticosa and Salvia ringens (Lamiaceae)" in Vojnosanitetski pregled, 79, no. 1 (2022):62-68,
https://doi.org/10.2298/VSP200508083S . .

TY  - CONF
AU  - Radović-Selgrad, Jelena
AU  - Ušjak, Dušan
AU  - Milenković, Marina
AU  - Kundaković-Vasović, Tatjana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4580
AB  - Dry methanol extract (DER 3.95:1) of aerial flowering parts of Alchemilla viridiflora
Rothm., Rosaceae, obtained after successive extraction with cyclohexane and
dichloromethane, predominantly contains polyphenols, tannins (3.74%), mostly ellagic, and
flavonoids (0.30%), hexosides of quercetin and kaempferol. It shows ACE inhibitory and
anti-Helicobacter pylori activity in vitro (1). Based on the traditional use of the common A.
vulgaris in the treatment of diabetes (2), the effect of this A. viridiflora extract on α-amylase
activity in vitro and lowering blood glucose in rats were investigated. α-Amylase inhibitory
activity was assessed using the 3,5-dinitrosalicylic acid method. Extract in doses of 50, 100
and 200 mg/kg, p.o., was administered to streptozotocin-induced diabetic rats for 20 days,
and blood glucose level and weight of rats were monitored during treatment. Extract
inhibited α-amylase activity at IC 50 5.47 ± 0.30 mg/mL. A dose of 200 mg/kg significantly
decreased blood glucose level after 10 (32.2%) and 20 days of treatment (38.3%). The effect
of this dose was similar to the effect of the reference drug, glibenclamide. A dose of 100
mg/kg significantly increased glucose level by day 10 (50.5%) and a reduction in blood sugar
level of 51.2% was observed only after 10 days. Body weight was correlated with changes in
blood sugar levels. A dose of 50 mg/kg had no statistically significant effect. Results indicate
a significant potential of A. viridiflora aerial parts in the treatment of diabetes, which must be
confirmed by additional studies, and active ingredients need to be identified.
AB  - Suvi metanolni ekstrakt (DER 3,95:1) nadzemnih delova u cvetu Alchemilla viridiflora
Rothm., Rosaceae, dobijen nakon sukcesivne ekstrakcije cikloheksanom i dihlormetanom,
sadrži visok nivo polifenola, tanina (3,74%), uglavnom elagnih, i flavonoida (0,30%),
heksozida kvercetina i kemferola. Pokazuje ACE inhibitornu i anti-Helicobacter pylori
aktivnost in vitro (1). Na osnovu podataka o tradicionalnoj upotrebi herbe A. vulgaris,
najčešće korišćene Alchemilla vrste, u lečenju dijabetesa (2), ispitivan je uticaj navedenog
ekstrakta A. viridiflora na aktivnost α-amilaze in vitro i na snižavanje nivoa glukoze u krvi
kod pacova. Uticaj na aktivnost α-amilaze procenjen je primenom metode sa 3,5-
dinitrosalicilnom kiselinom. Ekstrakt A. viridiflora u dozama od 50, 100 i 200 mg/kg, p.o.,
aplikovan je pacovima sa dijabetesom izazvanim streptozotocinom tokom 20 dana, a nivoi
glukoze u krvi i težina pacova su praćeni u određenim vremenskim intervalima tokom
trajanja tretmana. Ekstrakt je inhibirao aktivnost α-amilaze, a vrednost IC 50 iznosila je
5,47±0,30 mg/mL. Doza od 200 mg/kg značajno je smanjila nivo glukoze u krvi nakon 10
(32,2%) i 20 dana tretmana (38,3%). Efekat ove doze bio je sličan efektu referentnog leka,
glibenklamida. S druge strane, doza od 100 mg/kg značajno je povećala nivo glukoze do 10.
dana (50,5%) a do značajnog poboljšanja, tj. sniženja nivoa šećera od 51,2% dovela je tek
nakon 10 dana. Telesna težina bila je u korelaciji sa promenama nivoa šećera u krvi. Doza od
50 mg/kg nije imala statistički značajan efekat. Rezultati ukazuju na značajan potencijal
herbe A. viridiflora u lečenju dijabetesa, što mora biti potvrđeno dodatnim studijama, a
aktivni sastojci identifikovani.
PB  - Savez farmaceutskih udruženja Srbije (SFUS)
C3  - Arhiv za farmaciju
T1  - Inhibition of A-amylase in vitro and in vivo hypoglycemic effect of methanol extract of Alchemilla viridiflora Rothm
T1  - Inhibicija Α‐amilaze in vitro i in vivo hipoglikemijski efekat metanolnog ekstrakta herbe Alchemilla viridiflora Rothm
VL  - 72
IS  - 4 suplement
SP  - S461
EP  - S462
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4580
ER  - 

@conference{
author = "Radović-Selgrad, Jelena and Ušjak, Dušan and Milenković, Marina and Kundaković-Vasović, Tatjana",
year = "2022",
abstract = "Dry methanol extract (DER 3.95:1) of aerial flowering parts of Alchemilla viridiflora
Rothm., Rosaceae, obtained after successive extraction with cyclohexane and
dichloromethane, predominantly contains polyphenols, tannins (3.74%), mostly ellagic, and
flavonoids (0.30%), hexosides of quercetin and kaempferol. It shows ACE inhibitory and
anti-Helicobacter pylori activity in vitro (1). Based on the traditional use of the common A.
vulgaris in the treatment of diabetes (2), the effect of this A. viridiflora extract on α-amylase
activity in vitro and lowering blood glucose in rats were investigated. α-Amylase inhibitory
activity was assessed using the 3,5-dinitrosalicylic acid method. Extract in doses of 50, 100
and 200 mg/kg, p.o., was administered to streptozotocin-induced diabetic rats for 20 days,
and blood glucose level and weight of rats were monitored during treatment. Extract
inhibited α-amylase activity at IC 50 5.47 ± 0.30 mg/mL. A dose of 200 mg/kg significantly
decreased blood glucose level after 10 (32.2%) and 20 days of treatment (38.3%). The effect
of this dose was similar to the effect of the reference drug, glibenclamide. A dose of 100
mg/kg significantly increased glucose level by day 10 (50.5%) and a reduction in blood sugar
level of 51.2% was observed only after 10 days. Body weight was correlated with changes in
blood sugar levels. A dose of 50 mg/kg had no statistically significant effect. Results indicate
a significant potential of A. viridiflora aerial parts in the treatment of diabetes, which must be
confirmed by additional studies, and active ingredients need to be identified., Suvi metanolni ekstrakt (DER 3,95:1) nadzemnih delova u cvetu Alchemilla viridiflora
Rothm., Rosaceae, dobijen nakon sukcesivne ekstrakcije cikloheksanom i dihlormetanom,
sadrži visok nivo polifenola, tanina (3,74%), uglavnom elagnih, i flavonoida (0,30%),
heksozida kvercetina i kemferola. Pokazuje ACE inhibitornu i anti-Helicobacter pylori
aktivnost in vitro (1). Na osnovu podataka o tradicionalnoj upotrebi herbe A. vulgaris,
najčešće korišćene Alchemilla vrste, u lečenju dijabetesa (2), ispitivan je uticaj navedenog
ekstrakta A. viridiflora na aktivnost α-amilaze in vitro i na snižavanje nivoa glukoze u krvi
kod pacova. Uticaj na aktivnost α-amilaze procenjen je primenom metode sa 3,5-
dinitrosalicilnom kiselinom. Ekstrakt A. viridiflora u dozama od 50, 100 i 200 mg/kg, p.o.,
aplikovan je pacovima sa dijabetesom izazvanim streptozotocinom tokom 20 dana, a nivoi
glukoze u krvi i težina pacova su praćeni u određenim vremenskim intervalima tokom
trajanja tretmana. Ekstrakt je inhibirao aktivnost α-amilaze, a vrednost IC 50 iznosila je
5,47±0,30 mg/mL. Doza od 200 mg/kg značajno je smanjila nivo glukoze u krvi nakon 10
(32,2%) i 20 dana tretmana (38,3%). Efekat ove doze bio je sličan efektu referentnog leka,
glibenklamida. S druge strane, doza od 100 mg/kg značajno je povećala nivo glukoze do 10.
dana (50,5%) a do značajnog poboljšanja, tj. sniženja nivoa šećera od 51,2% dovela je tek
nakon 10 dana. Telesna težina bila je u korelaciji sa promenama nivoa šećera u krvi. Doza od
50 mg/kg nije imala statistički značajan efekat. Rezultati ukazuju na značajan potencijal
herbe A. viridiflora u lečenju dijabetesa, što mora biti potvrđeno dodatnim studijama, a
aktivni sastojci identifikovani.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "Inhibition of A-amylase in vitro and in vivo hypoglycemic effect of methanol extract of Alchemilla viridiflora Rothm, Inhibicija Α‐amilaze in vitro i in vivo hipoglikemijski efekat metanolnog ekstrakta herbe Alchemilla viridiflora Rothm",
volume = "72",
number = "4 suplement",
pages = "S461-S462",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4580"
}

Radović-Selgrad, J., Ušjak, D., Milenković, M.,& Kundaković-Vasović, T.. (2022). Inhibition of A-amylase in vitro and in vivo hypoglycemic effect of methanol extract of Alchemilla viridiflora Rothm. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije (SFUS)., 72(4 suplement), S461-S462.
https://hdl.handle.net/21.15107/rcub_farfar_4580

Radović-Selgrad J, Ušjak D, Milenković M, Kundaković-Vasović T. Inhibition of A-amylase in vitro and in vivo hypoglycemic effect of methanol extract of Alchemilla viridiflora Rothm. in Arhiv za farmaciju. 2022;72(4 suplement):S461-S462.
https://hdl.handle.net/21.15107/rcub_farfar_4580 .

Radović-Selgrad, Jelena, Ušjak, Dušan, Milenković, Marina, Kundaković-Vasović, Tatjana, "Inhibition of A-amylase in vitro and in vivo hypoglycemic effect of methanol extract of Alchemilla viridiflora Rothm" in Arhiv za farmaciju, 72, no. 4 suplement (2022):S461-S462,
https://hdl.handle.net/21.15107/rcub_farfar_4580 .

TY  - JOUR
AU  - Stevanović, Magdalena M.
AU  - Filipović, Nenad
AU  - Kuzmanović, Maja
AU  - Tomić, Nina
AU  - Ušjak, Dušan
AU  - Milenković, Marina
AU  - Zheng, Kai
AU  - Stampfl, Juergen
AU  - Boccaccini, Aldo R.
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4357
AB  - Multidrug-resistant bacterial strains represent an emerging global health threat and a great obstacle for bone tissue engineering. One of the major components of the extracellular matrix of the bone is a collagen protein, while selenium is an element that has antimicrobial potential, and is also important for bone metabolism and bone health. Here we represent the incorporation of selenium nanoparticles (SeNPs) synthesized by the green chemical reduction method into collagen gels to produce a composite material, collagen/SeNPs, with antimicrobial properties. The samples were comprehensively characterized by zeta potential measurements, dynamic light scattering inductively coupled plasma-mass spectrometry (ICP-MS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), optical microscopy, field-emission scanning electron microscopy (FE-SEM), and differential scanning calorimetry The cytotoxicity of the SeNPS, as well as collagen/SeNPs, was tested on the MRC-5 cells. It was revealed that collagen/SeNPS expressed a lower cytotoxic effect. Collagen/SeNPs showed significant antibacterial activity against all tested Gram-positive strains, the major causative agents of orthopedic infections as well as Candida albicans. Furthermore, three-dimensional β-tricalcium phosphate (3D-TCP) scaffolds were fabricated by a well-established 3D printing (lithography) method, and afterward preliminary coated by newly-synthesized SeNPs or collagen/SeNPs. In addition, uncoated 3D-TCP scaffolds as well as coated by collagen/SeNPs were subjected to biofilm formation. The production of Staphylococcus aureus biofilm on coated scaffolds by collagen/SeNPs was significantly reduced compared to the uncoated ones.
PB  - SAGE Publications Ltd
T2  - Journal of Biomaterials Applications
T1  - Synthesis and characterization of a collagen-based composite material containing selenium nanoparticles
VL  - 36
IS  - 10
SP  - 1800
EP  - 1811
DO  - 10.1177/08853282211073731
ER  - 

@article{
author = "Stevanović, Magdalena M. and Filipović, Nenad and Kuzmanović, Maja and Tomić, Nina and Ušjak, Dušan and Milenković, Marina and Zheng, Kai and Stampfl, Juergen and Boccaccini, Aldo R.",
year = "2022",
abstract = "Multidrug-resistant bacterial strains represent an emerging global health threat and a great obstacle for bone tissue engineering. One of the major components of the extracellular matrix of the bone is a collagen protein, while selenium is an element that has antimicrobial potential, and is also important for bone metabolism and bone health. Here we represent the incorporation of selenium nanoparticles (SeNPs) synthesized by the green chemical reduction method into collagen gels to produce a composite material, collagen/SeNPs, with antimicrobial properties. The samples were comprehensively characterized by zeta potential measurements, dynamic light scattering inductively coupled plasma-mass spectrometry (ICP-MS), X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), optical microscopy, field-emission scanning electron microscopy (FE-SEM), and differential scanning calorimetry The cytotoxicity of the SeNPS, as well as collagen/SeNPs, was tested on the MRC-5 cells. It was revealed that collagen/SeNPS expressed a lower cytotoxic effect. Collagen/SeNPs showed significant antibacterial activity against all tested Gram-positive strains, the major causative agents of orthopedic infections as well as Candida albicans. Furthermore, three-dimensional β-tricalcium phosphate (3D-TCP) scaffolds were fabricated by a well-established 3D printing (lithography) method, and afterward preliminary coated by newly-synthesized SeNPs or collagen/SeNPs. In addition, uncoated 3D-TCP scaffolds as well as coated by collagen/SeNPs were subjected to biofilm formation. The production of Staphylococcus aureus biofilm on coated scaffolds by collagen/SeNPs was significantly reduced compared to the uncoated ones.",
publisher = "SAGE Publications Ltd",
journal = "Journal of Biomaterials Applications",
title = "Synthesis and characterization of a collagen-based composite material containing selenium nanoparticles",
volume = "36",
number = "10",
pages = "1800-1811",
doi = "10.1177/08853282211073731"
}

Stevanović, M. M., Filipović, N., Kuzmanović, M., Tomić, N., Ušjak, D., Milenković, M., Zheng, K., Stampfl, J.,& Boccaccini, A. R.. (2022). Synthesis and characterization of a collagen-based composite material containing selenium nanoparticles. in Journal of Biomaterials Applications
SAGE Publications Ltd., 36(10), 1800-1811.
https://doi.org/10.1177/08853282211073731

Stevanović MM, Filipović N, Kuzmanović M, Tomić N, Ušjak D, Milenković M, Zheng K, Stampfl J, Boccaccini AR. Synthesis and characterization of a collagen-based composite material containing selenium nanoparticles. in Journal of Biomaterials Applications. 2022;36(10):1800-1811.
doi:10.1177/08853282211073731 .

Stevanović, Magdalena M., Filipović, Nenad, Kuzmanović, Maja, Tomić, Nina, Ušjak, Dušan, Milenković, Marina, Zheng, Kai, Stampfl, Juergen, Boccaccini, Aldo R., "Synthesis and characterization of a collagen-based composite material containing selenium nanoparticles" in Journal of Biomaterials Applications, 36, no. 10 (2022):1800-1811,
https://doi.org/10.1177/08853282211073731 . .

TY  - JOUR
AU  - Stevanović, Magdalena
AU  - Vukomanović, Marija
AU  - Milenković, Marina
AU  - Boccaccini, Aldo
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4280
PB  - Frontiers Media S.A.
T2  - Frontiers in Bioengineering and Biotechnology
T1  - Editorial: Antimicrobial nanostructured polymeric materials and nanocomposites, volume II
VL  - 10
DO  - 10.3389/fbioe.2022.1015485
ER  - 

@article{
author = "Stevanović, Magdalena and Vukomanović, Marija and Milenković, Marina and Boccaccini, Aldo",
year = "2022",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Bioengineering and Biotechnology",
title = "Editorial: Antimicrobial nanostructured polymeric materials and nanocomposites, volume II",
volume = "10",
doi = "10.3389/fbioe.2022.1015485"
}

Stevanović, M., Vukomanović, M., Milenković, M.,& Boccaccini, A.. (2022). Editorial: Antimicrobial nanostructured polymeric materials and nanocomposites, volume II. in Frontiers in Bioengineering and Biotechnology
Frontiers Media S.A.., 10.
https://doi.org/10.3389/fbioe.2022.1015485

Stevanović M, Vukomanović M, Milenković M, Boccaccini A. Editorial: Antimicrobial nanostructured polymeric materials and nanocomposites, volume II. in Frontiers in Bioengineering and Biotechnology. 2022;10.
doi:10.3389/fbioe.2022.1015485 .

Stevanović, Magdalena, Vukomanović, Marija, Milenković, Marina, Boccaccini, Aldo, "Editorial: Antimicrobial nanostructured polymeric materials and nanocomposites, volume II" in Frontiers in Bioengineering and Biotechnology, 10 (2022),
https://doi.org/10.3389/fbioe.2022.1015485 . .

TY  - JOUR
AU  - Ušjak, Dušan
AU  - Novović, Katarina
AU  - Filipić, Brankica
AU  - Kojić, Milan
AU  - Filipović, Nenad
AU  - Stevanović, Magdalena
AU  - Milenković, Marina
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4177
AB  - Aims: To investigate the synergistic activity of colistin and selenium nanoparticles (SeNPs) against pandrug-resistant (PDR) Ac. baumannii. Methods and Results: Chequerboard and time-kill assays were employed to ex- plore the potential synergistic interactions between colistin and SeNPs against Ac. baumannii isolates (8), previously determined as colistin-resistant (MIC range 16– 256 μg ml−1 ). Also, whole-genome sequencing (WGS) and gene expression analyses were used to elucidate the mechanisms of colistin resistance. Exceptionally strong synergistic activity (FICI range 0.004–0.035) of colistin and SeNPs against colistin- resistant isolates was revealed. Colistin (0.5 or 1 μg ml −1 ) used in combination with SeNPs (0.5 μg ml−1 ) was able to reduce initial inoculum during the first 4 h of incuba- tion, in contrast to colistin (0.5, 1 or 2 μg ml−1 ) alone. Conclusions: These findings propose colistin/SeNPs combination as a new option to fight PDR Ac. baumannii, the therapeutic possibilities of which should be proved in future in vivo studies. Significance and Impact of Study: Here we present the first evidence of synergy between colistin and selenium compounds against bacteria in general. Also, WGS and gene expression analyses provide some new insights into Ac. baumannii colistin resistance mechanisms.
PB  - John Wiley and Sons Inc
T2  - Journal of Applied Microbiology
T1  - In vitro colistin susceptibility of pandrug-resistant Ac. baumannii is restored in the presence of selenium nanoparticles
VL  - 133
IS  - 3
SP  - 1197
SP  - 1197
EP  - 1206
DO  - 10.1111/jam.15638
ER  - 

@article{
author = "Ušjak, Dušan and Novović, Katarina and Filipić, Brankica and Kojić, Milan and Filipović, Nenad and Stevanović, Magdalena and Milenković, Marina",
year = "2022",
abstract = "Aims: To investigate the synergistic activity of colistin and selenium nanoparticles (SeNPs) against pandrug-resistant (PDR) Ac. baumannii. Methods and Results: Chequerboard and time-kill assays were employed to ex- plore the potential synergistic interactions between colistin and SeNPs against Ac. baumannii isolates (8), previously determined as colistin-resistant (MIC range 16– 256 μg ml−1 ). Also, whole-genome sequencing (WGS) and gene expression analyses were used to elucidate the mechanisms of colistin resistance. Exceptionally strong synergistic activity (FICI range 0.004–0.035) of colistin and SeNPs against colistin- resistant isolates was revealed. Colistin (0.5 or 1 μg ml −1 ) used in combination with SeNPs (0.5 μg ml−1 ) was able to reduce initial inoculum during the first 4 h of incuba- tion, in contrast to colistin (0.5, 1 or 2 μg ml−1 ) alone. Conclusions: These findings propose colistin/SeNPs combination as a new option to fight PDR Ac. baumannii, the therapeutic possibilities of which should be proved in future in vivo studies. Significance and Impact of Study: Here we present the first evidence of synergy between colistin and selenium compounds against bacteria in general. Also, WGS and gene expression analyses provide some new insights into Ac. baumannii colistin resistance mechanisms.",
publisher = "John Wiley and Sons Inc",
journal = "Journal of Applied Microbiology",
title = "In vitro colistin susceptibility of pandrug-resistant Ac. baumannii is restored in the presence of selenium nanoparticles",
volume = "133",
number = "3",
pages = "1197-1197-1206",
doi = "10.1111/jam.15638"
}

Ušjak, D., Novović, K., Filipić, B., Kojić, M., Filipović, N., Stevanović, M.,& Milenković, M.. (2022). In vitro colistin susceptibility of pandrug-resistant Ac. baumannii is restored in the presence of selenium nanoparticles. in Journal of Applied Microbiology
John Wiley and Sons Inc., 133(3), 1197-1206.
https://doi.org/10.1111/jam.15638

Ušjak D, Novović K, Filipić B, Kojić M, Filipović N, Stevanović M, Milenković M. In vitro colistin susceptibility of pandrug-resistant Ac. baumannii is restored in the presence of selenium nanoparticles. in Journal of Applied Microbiology. 2022;133(3):1197-1206.
doi:10.1111/jam.15638 .

Ušjak, Dušan, Novović, Katarina, Filipić, Brankica, Kojić, Milan, Filipović, Nenad, Stevanović, Magdalena, Milenković, Marina, "In vitro colistin susceptibility of pandrug-resistant Ac. baumannii is restored in the presence of selenium nanoparticles" in Journal of Applied Microbiology, 133, no. 3 (2022):1197-1206,
https://doi.org/10.1111/jam.15638 . .

TY  - JOUR
AU  - Gajić, Ina
AU  - Kabić, Jovana
AU  - Kekić, Dušan
AU  - Jovićević, Miloš
AU  - Milenković, Marina
AU  - Mitić-Ćulafić, Dragana
AU  - Trudić, Anika
AU  - Ranin, Lazar
AU  - Opavski, Nataša
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4083
AB  - Antimicrobial resistance (AMR) has emerged as a major threat to public health globally. Accurate and rapid detection of resistance to antimicrobial drugs, and subsequent appropriate antimicrobial treatment, combined with antimicrobial stewardship, are essential for controlling the emergence and spread of AMR. This article reviews common antimicrobial susceptibility testing (AST) methods and relevant issues concerning the advantages and disadvantages of each method. Although accurate, classic technologies used in clinical microbiology to profile antimicrobial susceptibility are time-consuming and relatively expensive. As a result, physicians often prescribe empirical antimicrobial therapies and broad-spectrum antibiotics. Although recently developed AST systems have shown advantages over traditional methods in terms of testing speed and the potential for providing a deeper insight into resistance mechanisms, extensive validation is required to translate these methodologies to clinical practice. With a continuous increase in antimicrobial resistance, additional efforts are needed to develop innovative, rapid, accurate, and portable diagnostic tools for AST. The wide implementation of novel devices would enable the identification of the optimal treatment approaches and the surveillance of antibiotic resistance in health, agriculture, and the environment, allowing monitoring and better tackling the emergence of AMR.
PB  - MDPI
T2  - Antibiotics
T1  - Antimicrobial Susceptibility Testing: A Comprehensive Review of Currently Used Methods
VL  - 11
IS  - 4
DO  - 10.3390/antibiotics11040427
ER  - 

@article{
author = "Gajić, Ina and Kabić, Jovana and Kekić, Dušan and Jovićević, Miloš and Milenković, Marina and Mitić-Ćulafić, Dragana and Trudić, Anika and Ranin, Lazar and Opavski, Nataša",
year = "2022",
abstract = "Antimicrobial resistance (AMR) has emerged as a major threat to public health globally. Accurate and rapid detection of resistance to antimicrobial drugs, and subsequent appropriate antimicrobial treatment, combined with antimicrobial stewardship, are essential for controlling the emergence and spread of AMR. This article reviews common antimicrobial susceptibility testing (AST) methods and relevant issues concerning the advantages and disadvantages of each method. Although accurate, classic technologies used in clinical microbiology to profile antimicrobial susceptibility are time-consuming and relatively expensive. As a result, physicians often prescribe empirical antimicrobial therapies and broad-spectrum antibiotics. Although recently developed AST systems have shown advantages over traditional methods in terms of testing speed and the potential for providing a deeper insight into resistance mechanisms, extensive validation is required to translate these methodologies to clinical practice. With a continuous increase in antimicrobial resistance, additional efforts are needed to develop innovative, rapid, accurate, and portable diagnostic tools for AST. The wide implementation of novel devices would enable the identification of the optimal treatment approaches and the surveillance of antibiotic resistance in health, agriculture, and the environment, allowing monitoring and better tackling the emergence of AMR.",
publisher = "MDPI",
journal = "Antibiotics",
title = "Antimicrobial Susceptibility Testing: A Comprehensive Review of Currently Used Methods",
volume = "11",
number = "4",
doi = "10.3390/antibiotics11040427"
}

Gajić, I., Kabić, J., Kekić, D., Jovićević, M., Milenković, M., Mitić-Ćulafić, D., Trudić, A., Ranin, L.,& Opavski, N.. (2022). Antimicrobial Susceptibility Testing: A Comprehensive Review of Currently Used Methods. in Antibiotics
MDPI., 11(4).
https://doi.org/10.3390/antibiotics11040427

Gajić I, Kabić J, Kekić D, Jovićević M, Milenković M, Mitić-Ćulafić D, Trudić A, Ranin L, Opavski N. Antimicrobial Susceptibility Testing: A Comprehensive Review of Currently Used Methods. in Antibiotics. 2022;11(4).
doi:10.3390/antibiotics11040427 .

Gajić, Ina, Kabić, Jovana, Kekić, Dušan, Jovićević, Miloš, Milenković, Marina, Mitić-Ćulafić, Dragana, Trudić, Anika, Ranin, Lazar, Opavski, Nataša, "Antimicrobial Susceptibility Testing: A Comprehensive Review of Currently Used Methods" in Antibiotics, 11, no. 4 (2022),
https://doi.org/10.3390/antibiotics11040427 . .

TY  - CONF
AU  - Ušjak, Ljuboš
AU  - Drobac, Milica
AU  - Ivanov, Marija
AU  - Soković, Marina
AU  - Milenković, Marina
AU  - Niketić, Marjan
AU  - Petrović, Silvana
PY  - 2021-11
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4615
AB  - INTRODUCTION:
Cultivated parsnip (Pastinaca sativa subsp. sativa L., Apiaceae) root is a well-known vegetable, common ingredient of soups, stews, salads, casseroles etc. Besides, its leaves and young shoots can be added to soups and fruits are used as a condiment. Furthermore, young shoots of wild-growing parsnips, e.g. P. sativa subsp. urens (Req. ex Godr.) Čelak., are consumed pickled
or in salads or soups and it is considered that their essential oil acts as a natural preservative.

OBJECTIVES:
To investigate and compare the antimicrobial activity of the essential oils obtained from the roots, leaves, stems, flowers and fruits of cultivated P. sativa subsp. sativa and wild-growing P. sativa subsp. urens collected in Serbia. Furthermore, the most active essential oil constituents (against the most susceptible microorganisms) were predicted in silico.

METHOD / DESIGN:
Minimum inhibitory concentrations (MICs) and minimum bactericidal/fungicidal concentrations (MBCs/MFCs) of the essential oils (isolated by hydrodistillation using Clevenger-type apparatus) were determined by microdilution method against three Gram-positive bacteria: Staphylococcus aureus ATCC 11632, Bacillus cereus clinical isolate and Listeria monocytogenes NCTC 7973, three Gram-negative bacteria: Escherichia coli ATCC 25922, Salmonella Typhimurium ATCC 13311 and Enterobacter cloacae ATCC 35030, three Candida standard strains: C. albicans ATCC 10231, C. tropicalis ATCC 750 and C. parapsilosis ATCC 22019, and three Candida isolates from oral cavity: C. albicans 475/15, C. krusei H1/16 and C. glabrata 4/6/15. Pharmacokinetic properties of the compounds present in at least one oil in the quantity ≥ 1% (determined by GC-FID and GC-MS) were initially evaluated using SwissADME web tool and molecular docking was performed using AutoDock Vina 1.1.2 (interactions were visualized using Discovery Studio Visualizer 2019).

RESULTS:
All the investigated essential oils of the two Pastinaca sativa subspecies were able to reduce the growth of different tested Candida strains (MIC range 0.25-2 mg/mL; MFC range 0.5-4 mg/mL). The most promising activity was observed for both root oils (MIC range 0.25-1 mg/mL; MFC range 0.5-2 mg/mL). Among investigated Candida strains, C. parapsilosis strain was the most sensitive to these essential oils (MIC range 0.25-1 mg/mL; MFC range 0.5-2 mg/mL). The antibacterial activity of the tested essential oils was lower compared to their anticandidal potential (MIC range 1-4 mg/mL; MBC range 2-8 mg/mL). Thirty compounds were present in at least one oil in the quantity ≥ 1%. Estimation of pharmacokinetic properties using SwissADME tool suggested that 23 of these compounds are inhibitors of some of the cytochrome P450 system isoenzymes. This fact led
to assumption that they could also act against fungal sterol 14α-demethylase (CYP51), which is a common target of antifungal drugs (e.g., ketoconazole). Thus, the compounds (3D structures downloaded from PubChem) were docked to the active site of this enzyme (downloaded from Protein Data Bank, PDB code 5TZ1). The highest affinities were predicted for sesquiterpenes
caryophyllene oxide, (E)-caryophyllene, germacrene D, α-copaene, β-bourbonene and δ-cadinene (free binding energies from -9.4 to -8.7 kcal/mol; ketoconazole -11.6 kcal/mol). These compounds were present in somewhat lower quantities in the essential oils (≤ 9.9%). For dominant compounds of the tested essential oils, e.g. myristicin, γ-palmitolactone and octyl butanoate a bit lower affinities were predicted (free binding energies from -7.3 to -5.8 kcal/mol). Tested compounds mostly docked near the heme of the enzyme and formed hydrophobic interactions with the amino acid residues of the active site. According to SwissADME tool, four of five most active compounds have low absorption from gastrointestinal tract and higher skin permeation value, while caryophyllene oxide and three dominant compounds have high absorption and lower skin permeation value (similarly to ketoconazole).

CONCLUSIONS:
Investigated parsnips represent sources of essential oils and compounds with anticandidal activity.
PB  - University Prince of Songkla, Thailand
PB  - University of Novi Sad, Faculty of Sciences, Serbia, Novi Sad
C3  - The International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference - IBSC2021 Book of Abstracts
T1  - In vitro and in silico investingation of antimicrobial activity of essential oils from two Pastinaca sativa subspecies
SP  - 188
EP  - 189
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4615
ER  - 

@conference{
author = "Ušjak, Ljuboš and Drobac, Milica and Ivanov, Marija and Soković, Marina and Milenković, Marina and Niketić, Marjan and Petrović, Silvana",
year = "2021-11",
abstract = "INTRODUCTION:
Cultivated parsnip (Pastinaca sativa subsp. sativa L., Apiaceae) root is a well-known vegetable, common ingredient of soups, stews, salads, casseroles etc. Besides, its leaves and young shoots can be added to soups and fruits are used as a condiment. Furthermore, young shoots of wild-growing parsnips, e.g. P. sativa subsp. urens (Req. ex Godr.) Čelak., are consumed pickled
or in salads or soups and it is considered that their essential oil acts as a natural preservative.

OBJECTIVES:
To investigate and compare the antimicrobial activity of the essential oils obtained from the roots, leaves, stems, flowers and fruits of cultivated P. sativa subsp. sativa and wild-growing P. sativa subsp. urens collected in Serbia. Furthermore, the most active essential oil constituents (against the most susceptible microorganisms) were predicted in silico.

METHOD / DESIGN:
Minimum inhibitory concentrations (MICs) and minimum bactericidal/fungicidal concentrations (MBCs/MFCs) of the essential oils (isolated by hydrodistillation using Clevenger-type apparatus) were determined by microdilution method against three Gram-positive bacteria: Staphylococcus aureus ATCC 11632, Bacillus cereus clinical isolate and Listeria monocytogenes NCTC 7973, three Gram-negative bacteria: Escherichia coli ATCC 25922, Salmonella Typhimurium ATCC 13311 and Enterobacter cloacae ATCC 35030, three Candida standard strains: C. albicans ATCC 10231, C. tropicalis ATCC 750 and C. parapsilosis ATCC 22019, and three Candida isolates from oral cavity: C. albicans 475/15, C. krusei H1/16 and C. glabrata 4/6/15. Pharmacokinetic properties of the compounds present in at least one oil in the quantity ≥ 1% (determined by GC-FID and GC-MS) were initially evaluated using SwissADME web tool and molecular docking was performed using AutoDock Vina 1.1.2 (interactions were visualized using Discovery Studio Visualizer 2019).

RESULTS:
All the investigated essential oils of the two Pastinaca sativa subspecies were able to reduce the growth of different tested Candida strains (MIC range 0.25-2 mg/mL; MFC range 0.5-4 mg/mL). The most promising activity was observed for both root oils (MIC range 0.25-1 mg/mL; MFC range 0.5-2 mg/mL). Among investigated Candida strains, C. parapsilosis strain was the most sensitive to these essential oils (MIC range 0.25-1 mg/mL; MFC range 0.5-2 mg/mL). The antibacterial activity of the tested essential oils was lower compared to their anticandidal potential (MIC range 1-4 mg/mL; MBC range 2-8 mg/mL). Thirty compounds were present in at least one oil in the quantity ≥ 1%. Estimation of pharmacokinetic properties using SwissADME tool suggested that 23 of these compounds are inhibitors of some of the cytochrome P450 system isoenzymes. This fact led
to assumption that they could also act against fungal sterol 14α-demethylase (CYP51), which is a common target of antifungal drugs (e.g., ketoconazole). Thus, the compounds (3D structures downloaded from PubChem) were docked to the active site of this enzyme (downloaded from Protein Data Bank, PDB code 5TZ1). The highest affinities were predicted for sesquiterpenes
caryophyllene oxide, (E)-caryophyllene, germacrene D, α-copaene, β-bourbonene and δ-cadinene (free binding energies from -9.4 to -8.7 kcal/mol; ketoconazole -11.6 kcal/mol). These compounds were present in somewhat lower quantities in the essential oils (≤ 9.9%). For dominant compounds of the tested essential oils, e.g. myristicin, γ-palmitolactone and octyl butanoate a bit lower affinities were predicted (free binding energies from -7.3 to -5.8 kcal/mol). Tested compounds mostly docked near the heme of the enzyme and formed hydrophobic interactions with the amino acid residues of the active site. According to SwissADME tool, four of five most active compounds have low absorption from gastrointestinal tract and higher skin permeation value, while caryophyllene oxide and three dominant compounds have high absorption and lower skin permeation value (similarly to ketoconazole).

CONCLUSIONS:
Investigated parsnips represent sources of essential oils and compounds with anticandidal activity.",
publisher = "University Prince of Songkla, Thailand, University of Novi Sad, Faculty of Sciences, Serbia, Novi Sad",
journal = "The International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference - IBSC2021 Book of Abstracts",
title = "In vitro and in silico investingation of antimicrobial activity of essential oils from two Pastinaca sativa subspecies",
pages = "188-189",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4615"
}

Ušjak, L., Drobac, M., Ivanov, M., Soković, M., Milenković, M., Niketić, M.,& Petrović, S.. (2021-11). In vitro and in silico investingation of antimicrobial activity of essential oils from two Pastinaca sativa subspecies. in The International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference - IBSC2021 Book of Abstracts
University Prince of Songkla, Thailand., 188-189.
https://hdl.handle.net/21.15107/rcub_farfar_4615

Ušjak L, Drobac M, Ivanov M, Soković M, Milenković M, Niketić M, Petrović S. In vitro and in silico investingation of antimicrobial activity of essential oils from two Pastinaca sativa subspecies. in The International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference - IBSC2021 Book of Abstracts. 2021;:188-189.
https://hdl.handle.net/21.15107/rcub_farfar_4615 .

Ušjak, Ljuboš, Drobac, Milica, Ivanov, Marija, Soković, Marina, Milenković, Marina, Niketić, Marjan, Petrović, Silvana, "In vitro and in silico investingation of antimicrobial activity of essential oils from two Pastinaca sativa subspecies" in The International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference - IBSC2021 Book of Abstracts (2021-11):188-189,
https://hdl.handle.net/21.15107/rcub_farfar_4615 .

TY  - JOUR
AU  - Ilić, Milan
AU  - Samardžić, Stevan
AU  - Kotur-Stevuljević, Jelena
AU  - Ušjak, Dušan
AU  - Milenković, Marina
AU  - Kovačević, Nada
AU  - Drobac, Milica
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3995
AB  - Plants and plant extracts are of great scientific interest due to the chemical diversity and pharmacological properties of present bioactive molecules. The Geranium L . species are widely used in ethnomedicine. In the current study, the total phenolic and tannin content, antioxidant and antimicrobial activity of methanol extracts of eight Geranium species were investigated.
PB  - Verduci Editore s.r.l
T2  - European Review for Medical and Pharmacological Sciences
T1  - Polyphenol rich extracts of Geranium L. species as potential natural antioxidant and antimicrobial agents
VL  - 25
IS  - 20
SP  - 6283
EP  - 6294
DO  - 10.26355/eurrev_202110_26998
ER  - 

@article{
author = "Ilić, Milan and Samardžić, Stevan and Kotur-Stevuljević, Jelena and Ušjak, Dušan and Milenković, Marina and Kovačević, Nada and Drobac, Milica",
year = "2021",
abstract = "Plants and plant extracts are of great scientific interest due to the chemical diversity and pharmacological properties of present bioactive molecules. The Geranium L . species are widely used in ethnomedicine. In the current study, the total phenolic and tannin content, antioxidant and antimicrobial activity of methanol extracts of eight Geranium species were investigated.",
publisher = "Verduci Editore s.r.l",
journal = "European Review for Medical and Pharmacological Sciences",
title = "Polyphenol rich extracts of Geranium L. species as potential natural antioxidant and antimicrobial agents",
volume = "25",
number = "20",
pages = "6283-6294",
doi = "10.26355/eurrev_202110_26998"
}

Ilić, M., Samardžić, S., Kotur-Stevuljević, J., Ušjak, D., Milenković, M., Kovačević, N.,& Drobac, M.. (2021). Polyphenol rich extracts of Geranium L. species as potential natural antioxidant and antimicrobial agents. in European Review for Medical and Pharmacological Sciences
Verduci Editore s.r.l., 25(20), 6283-6294.
https://doi.org/10.26355/eurrev_202110_26998

Ilić M, Samardžić S, Kotur-Stevuljević J, Ušjak D, Milenković M, Kovačević N, Drobac M. Polyphenol rich extracts of Geranium L. species as potential natural antioxidant and antimicrobial agents. in European Review for Medical and Pharmacological Sciences. 2021;25(20):6283-6294.
doi:10.26355/eurrev_202110_26998 .

Ilić, Milan, Samardžić, Stevan, Kotur-Stevuljević, Jelena, Ušjak, Dušan, Milenković, Marina, Kovačević, Nada, Drobac, Milica, "Polyphenol rich extracts of Geranium L. species as potential natural antioxidant and antimicrobial agents" in European Review for Medical and Pharmacological Sciences, 25, no. 20 (2021):6283-6294,
https://doi.org/10.26355/eurrev_202110_26998 . .

TY  - JOUR
AU  - Suručić, Relja
AU  - Kundaković-Vasović, Tatjana
AU  - Marčetić, Mirjana
AU  - Drakul, Dragana
AU  - Milenković, Marina
AU  - Kovačević, Nada
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4002
AB  - Phytochemical and pharmacological in-vestigations of essential oils isolated from plant species of the genus Seseli have been intensified recently. These plant species have long-term use in nutrition and traditional medicine in the treatment of various disorders. Volatile secondary metabolites of Seseli gracile Waldst. & Kit (Apiaceae) have not been pharma-cologically examined so far. The aim of the conducted research was to assess the antiradical, antimicrobial and spasmolytic ac-tivities of S. gracile essential oil isolated from the aerial parts of the plant. Methods. The antiradical activity was determined us-ing the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scaveng-ing test, antimicrobial activity using broth microdilution meth-od with standard strains of Gram (+), Gram (-) bacteria and yeast, while spasmolytic activity was evaluated on isolated rat il-eum, pre-contracted with a high concentration of potassium. Results. The results showed moderate antiradical (SC50 = 78.60 μg/mL) and good spasmolytic activity (IC50 = 271.4 nL/mL) of S. gracile essential oil. In the range of tested concen-trations, minimal inhibitory concentration (MIC) was deter-mined only for the strain of Escherichia coli (500 μg/mL). Con-clusion. Results obtained in this study justify the need for fur-ther studies to elucidate exact molecular mechanism underlay this spasmolytic effect of S. gracile herb essential oil.
AB  - Etarska ulja izolovana iz vrsta roda Seseli su u poslednje vreme intenzivno farmakološki i fitohemijski proučavana. Biljke iz ovog roda se koriste već duži niz godina u ishrani i tradicionalnoj terapiji različitih oboljenja. Farmakološka aktivnost etarskog ulja izolovanog iz Seseli gracile do sada nije ispitivana. Stoga je cilj ovog istraživanja bio da se ispita antiradikalska, antimikrobna i spazmolitična aktivnost etarskog ulja izolovanog iz nadzemnih delova biljne vrste S. gracile. Metode. Antiradikalska aktivnost je utvrđena testom neutralizacije 2.2-difenil-1-pikrilhidrazil (DPPH) radikala, antimikrobna, bujon-mikrodilucionom metodom uz upotrebu standardnih sojeva Gram (+), Gram (-) bakterija i gljivica, a spazmolitična na izolovanom ileumu pacova, prethodno kontrahovanom visokom koncentarcijom kalijuma. Rezultati. Rezultati pokazuju umerenu antiradikalsku (SC50 = 78.60 μg/mL) i dobru spazmolitičnu aktivnost (IC50 = 271.4 nL/mL) etarskog ulja S. gracile. U opsegu testiranih koncentracija utvrđena je minimalna inhibitorna koncentracija (MIC) samo za soj Escherichia coli (500 μg/mL). Zaključak. Dobijeni rezultati opravdavaju potrebu za budućim istraživanjima koja bi razjasnila tačne mehanizme kojima se ostvaruje spazmolitički efekat etarskog ulja nadzemnog dela S. gracile.
PB  - Belgrade : Military Medical Academy, INI
T2  - Vojnosanitetski pregled
T1  - Pharmacological characterisation of Seseli gracile Waldst. & Kit. (Apiaceae) essential oil
T1  - Farmakološka karakterizacija etarskog ulja Seseli gracile Waldst. & Kit. (Apiaceae)
VL  - 78
IS  - 10
SP  - 1065
EP  - 1071
DO  - 10.2298/VSP190320028S
ER  - 

@article{
author = "Suručić, Relja and Kundaković-Vasović, Tatjana and Marčetić, Mirjana and Drakul, Dragana and Milenković, Marina and Kovačević, Nada",
year = "2021",
abstract = "Phytochemical and pharmacological in-vestigations of essential oils isolated from plant species of the genus Seseli have been intensified recently. These plant species have long-term use in nutrition and traditional medicine in the treatment of various disorders. Volatile secondary metabolites of Seseli gracile Waldst. & Kit (Apiaceae) have not been pharma-cologically examined so far. The aim of the conducted research was to assess the antiradical, antimicrobial and spasmolytic ac-tivities of S. gracile essential oil isolated from the aerial parts of the plant. Methods. The antiradical activity was determined us-ing the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scaveng-ing test, antimicrobial activity using broth microdilution meth-od with standard strains of Gram (+), Gram (-) bacteria and yeast, while spasmolytic activity was evaluated on isolated rat il-eum, pre-contracted with a high concentration of potassium. Results. The results showed moderate antiradical (SC50 = 78.60 μg/mL) and good spasmolytic activity (IC50 = 271.4 nL/mL) of S. gracile essential oil. In the range of tested concen-trations, minimal inhibitory concentration (MIC) was deter-mined only for the strain of Escherichia coli (500 μg/mL). Con-clusion. Results obtained in this study justify the need for fur-ther studies to elucidate exact molecular mechanism underlay this spasmolytic effect of S. gracile herb essential oil., Etarska ulja izolovana iz vrsta roda Seseli su u poslednje vreme intenzivno farmakološki i fitohemijski proučavana. Biljke iz ovog roda se koriste već duži niz godina u ishrani i tradicionalnoj terapiji različitih oboljenja. Farmakološka aktivnost etarskog ulja izolovanog iz Seseli gracile do sada nije ispitivana. Stoga je cilj ovog istraživanja bio da se ispita antiradikalska, antimikrobna i spazmolitična aktivnost etarskog ulja izolovanog iz nadzemnih delova biljne vrste S. gracile. Metode. Antiradikalska aktivnost je utvrđena testom neutralizacije 2.2-difenil-1-pikrilhidrazil (DPPH) radikala, antimikrobna, bujon-mikrodilucionom metodom uz upotrebu standardnih sojeva Gram (+), Gram (-) bakterija i gljivica, a spazmolitična na izolovanom ileumu pacova, prethodno kontrahovanom visokom koncentarcijom kalijuma. Rezultati. Rezultati pokazuju umerenu antiradikalsku (SC50 = 78.60 μg/mL) i dobru spazmolitičnu aktivnost (IC50 = 271.4 nL/mL) etarskog ulja S. gracile. U opsegu testiranih koncentracija utvrđena je minimalna inhibitorna koncentracija (MIC) samo za soj Escherichia coli (500 μg/mL). Zaključak. Dobijeni rezultati opravdavaju potrebu za budućim istraživanjima koja bi razjasnila tačne mehanizme kojima se ostvaruje spazmolitički efekat etarskog ulja nadzemnog dela S. gracile.",
publisher = "Belgrade : Military Medical Academy, INI",
journal = "Vojnosanitetski pregled",
title = "Pharmacological characterisation of Seseli gracile Waldst. & Kit. (Apiaceae) essential oil, Farmakološka karakterizacija etarskog ulja Seseli gracile Waldst. & Kit. (Apiaceae)",
volume = "78",
number = "10",
pages = "1065-1071",
doi = "10.2298/VSP190320028S"
}

Suručić, R., Kundaković-Vasović, T., Marčetić, M., Drakul, D., Milenković, M.,& Kovačević, N.. (2021). Pharmacological characterisation of Seseli gracile Waldst. & Kit. (Apiaceae) essential oil. in Vojnosanitetski pregled
Belgrade : Military Medical Academy, INI., 78(10), 1065-1071.
https://doi.org/10.2298/VSP190320028S

Suručić R, Kundaković-Vasović T, Marčetić M, Drakul D, Milenković M, Kovačević N. Pharmacological characterisation of Seseli gracile Waldst. & Kit. (Apiaceae) essential oil. in Vojnosanitetski pregled. 2021;78(10):1065-1071.
doi:10.2298/VSP190320028S .

Suručić, Relja, Kundaković-Vasović, Tatjana, Marčetić, Mirjana, Drakul, Dragana, Milenković, Marina, Kovačević, Nada, "Pharmacological characterisation of Seseli gracile Waldst. & Kit. (Apiaceae) essential oil" in Vojnosanitetski pregled, 78, no. 10 (2021):1065-1071,
https://doi.org/10.2298/VSP190320028S . .

TY  - JOUR
AU  - Drobac, Milica
AU  - Kukić-Marković, Jelena
AU  - Milenković, Marina
AU  - Niketić, Marjan
AU  - Petrović, Silvana
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4000
AB  - Aromatic plants and essential oils have many applications in medicine, pharmaceuticals, cosmetics, and the food industry. The essential oil of the flowering aerial parts of Achillea grandifolia, obtained by hydrodistillation, was analyzed for its constituents and investigated for antimicrobial and radical scavenging activity. The essential oil was characterized by a high amount of oxygenated monoterpenes (72.7%) with 1,8-cineole (29.2%) and camphor (23.4%) being the most abundant. Sesquiterpenes were present in smaller quantities (4.8%). Antimicrobial activity was tested against eight ATCC bacterial strains and two ATCC strains of Candida albicans. The essential oil exhibited highly pronounced antimicrobial activity against Micrococcus luteus with a MIC value of 3.50 μg/mL, as well as significant antimicrobial activity (<100 μg/mL) against Staphylococcus aureus, S. epidermidis and Bacillus subtilis. Gram-negative bacteria Escherichia coli and Pseudomonas aeruginosa were resistant. Achillea grandifolia essential oil exhibited concentration-dependent antiradical activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical with an SC50 value of 5.4 mg/mL. The TLC-DPPH assay revealed two main light yellow spots indicating components with anti-DPPH activity, which after isolation were identified as 1,8-cineole and camphor.
AB  - Aromatične biljke i etarska ulja nalaze primenu u medicini, farmaciji, kozmetičkoj, parfimerijskoj i prehrambenoj industriji. Etarsko ulje iz nadzemnih delova u cvetu Achillea grandifolia izolovano je destilacijom vodenom parom i ispitivano u pogledu hemijskog sastava, antimikrobne i antiradikalske aktivnosti. Etarsko ulje se odlikovalo velikom količinom oksidovanih monoterpena (72,7%) sa 1,8-cineolom (29,2%) i kamforom (23,4%) kao dominantnim jedinjenjima. Seskviterpeni su bili prisutni u manjoj količini (4,8%). Antimikrobna aktivnost je ispitivana prema osam ATCC sojeva bakterija i dva ATCC soja Candida albicans. Etarsko ulje je pokazalo veoma izraženu antimikrobnu aktivnost prema Micrococcus luteus sa MIC vrednošću od 3,50 μg/mL, kao i značajnu aktivnost prema Staphylococcus aureus, S. epidermidis i Bacillus subtilis. Gram-negativne bakterije Escherichia coli i Pseudomonas aeruginosa bile su rezistentne. Etarsko ulje je ispoljilo dozno-zavisnu antiradikalsku aktivnost prema DPPH radikalu sa SC50 vrednošću od 5,4 mg/mL. U TLC-DPPH testu uočene su dve glavne žute zone koje odgovaraju anti-DPPH aktivnim jedinjenjima, a koja su nakon izolovanja identifikovana kao 1,8-cineol i kamfor.
PB  - Institute of Botany and Botanical Garden "Jevremovac", University of
T2  - Botanica Serbica
T1  - The chemical composition, antimicrobial and antiradical properties of the essential oil of Achillea grandifolia aerial parts from Serbia
T1  - Hemijski sastav, antimikrobna i antiradikalska svojstva etarskog ulja nadzemnih delova Achillea grandifolia iz Srbije
VL  - 45
IS  - 2
SP  - 233
EP  - 240
DO  - 10.2298/BOTSERB2102233D
ER  - 

@article{
author = "Drobac, Milica and Kukić-Marković, Jelena and Milenković, Marina and Niketić, Marjan and Petrović, Silvana",
year = "2021",
abstract = "Aromatic plants and essential oils have many applications in medicine, pharmaceuticals, cosmetics, and the food industry. The essential oil of the flowering aerial parts of Achillea grandifolia, obtained by hydrodistillation, was analyzed for its constituents and investigated for antimicrobial and radical scavenging activity. The essential oil was characterized by a high amount of oxygenated monoterpenes (72.7%) with 1,8-cineole (29.2%) and camphor (23.4%) being the most abundant. Sesquiterpenes were present in smaller quantities (4.8%). Antimicrobial activity was tested against eight ATCC bacterial strains and two ATCC strains of Candida albicans. The essential oil exhibited highly pronounced antimicrobial activity against Micrococcus luteus with a MIC value of 3.50 μg/mL, as well as significant antimicrobial activity (<100 μg/mL) against Staphylococcus aureus, S. epidermidis and Bacillus subtilis. Gram-negative bacteria Escherichia coli and Pseudomonas aeruginosa were resistant. Achillea grandifolia essential oil exhibited concentration-dependent antiradical activity against 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical with an SC50 value of 5.4 mg/mL. The TLC-DPPH assay revealed two main light yellow spots indicating components with anti-DPPH activity, which after isolation were identified as 1,8-cineole and camphor., Aromatične biljke i etarska ulja nalaze primenu u medicini, farmaciji, kozmetičkoj, parfimerijskoj i prehrambenoj industriji. Etarsko ulje iz nadzemnih delova u cvetu Achillea grandifolia izolovano je destilacijom vodenom parom i ispitivano u pogledu hemijskog sastava, antimikrobne i antiradikalske aktivnosti. Etarsko ulje se odlikovalo velikom količinom oksidovanih monoterpena (72,7%) sa 1,8-cineolom (29,2%) i kamforom (23,4%) kao dominantnim jedinjenjima. Seskviterpeni su bili prisutni u manjoj količini (4,8%). Antimikrobna aktivnost je ispitivana prema osam ATCC sojeva bakterija i dva ATCC soja Candida albicans. Etarsko ulje je pokazalo veoma izraženu antimikrobnu aktivnost prema Micrococcus luteus sa MIC vrednošću od 3,50 μg/mL, kao i značajnu aktivnost prema Staphylococcus aureus, S. epidermidis i Bacillus subtilis. Gram-negativne bakterije Escherichia coli i Pseudomonas aeruginosa bile su rezistentne. Etarsko ulje je ispoljilo dozno-zavisnu antiradikalsku aktivnost prema DPPH radikalu sa SC50 vrednošću od 5,4 mg/mL. U TLC-DPPH testu uočene su dve glavne žute zone koje odgovaraju anti-DPPH aktivnim jedinjenjima, a koja su nakon izolovanja identifikovana kao 1,8-cineol i kamfor.",
publisher = "Institute of Botany and Botanical Garden "Jevremovac", University of",
journal = "Botanica Serbica",
title = "The chemical composition, antimicrobial and antiradical properties of the essential oil of Achillea grandifolia aerial parts from Serbia, Hemijski sastav, antimikrobna i antiradikalska svojstva etarskog ulja nadzemnih delova Achillea grandifolia iz Srbije",
volume = "45",
number = "2",
pages = "233-240",
doi = "10.2298/BOTSERB2102233D"
}

Drobac, M., Kukić-Marković, J., Milenković, M., Niketić, M.,& Petrović, S.. (2021). The chemical composition, antimicrobial and antiradical properties of the essential oil of Achillea grandifolia aerial parts from Serbia. in Botanica Serbica
Institute of Botany and Botanical Garden "Jevremovac", University of., 45(2), 233-240.
https://doi.org/10.2298/BOTSERB2102233D

Drobac M, Kukić-Marković J, Milenković M, Niketić M, Petrović S. The chemical composition, antimicrobial and antiradical properties of the essential oil of Achillea grandifolia aerial parts from Serbia. in Botanica Serbica. 2021;45(2):233-240.
doi:10.2298/BOTSERB2102233D .

Drobac, Milica, Kukić-Marković, Jelena, Milenković, Marina, Niketić, Marjan, Petrović, Silvana, "The chemical composition, antimicrobial and antiradical properties of the essential oil of Achillea grandifolia aerial parts from Serbia" in Botanica Serbica, 45, no. 2 (2021):233-240,
https://doi.org/10.2298/BOTSERB2102233D . .

TY  - CONF
AU  - Obradović, Bojana
AU  - Stojkovska, Jasmina
AU  - Osmokrović, Andrea
AU  - Mišković Stanković, Vesna
AU  - Jančić, Ivan
AU  - Milenković, Marina
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4666
AB  - Alginate hydrogels are widely used in wound dressings due to hydrophilicity, biocompatibility,
flexibility, and high sorption capacity, providing effective moisture regulation in wounds and inducing
rapid granulation and reepithelization of the damaged tissue. However, these dressings are not
bioactive so that different methodologies have been investigated to extend functionality of alginate
hydrogels.
In the present work, we show several approaches to achieve this aim by addition of different
biologically active components. These include incorporation of silver nanoparticles as potent
antimicrobial agents (1), bioactive honey components (2), activated charcoal (AC) particles as carriers
of therapeutically active agents (3) as well as the use of Zn-alginate hydrogels that release zinc ions (4).
The obtained composites were comprehensively characterized regarding composition, cytotoxicity,
antibacterial activity, release kinetics of active agents and wound treatment in a rat model.
Ag/alginate nanocomposite hydrogels releasing silver ions and/or nanoparticles exhibited high
bactericidal activity against a broad spectrum of standard and multi-drug resistant clinical bacterial
strains (Escherichia coli, Staphylococcus aureus, methicillin-resistant Staphylococcus aureus – MRSA,
Acinetobacter baumannii and Pseudomonas aeruginosa). Especially interesting results were obtained
against 13 clinical isolates of A. baumannii, which were completely extinguished over 48 h in 6 cases
(2). However, in 3 clinical isolates, antibacterial effects were not noticed implying possibility for
development of bacterial resistance to silver. In the treatment of 2nd degree burns in rats Ag/alginate
nanocomposites exhibited the same efficiency as commercial medical products (5).
Composite alginate hydrogels with immobilized AC particles impregnated with povidone iodine
(PVP-I) as a model therapeutically active agent, were developed with the aim to provide controlled
particle release in the wound without actually releasing the adsorbed substance, thus achieving the
desired activity without adverse effects by systemic absorption. The composite Ca-alginate hydrogels
induced strong bactericidal effects against two standard bacterial strains and clinical multi-resistant
wound isolates (MRSA, E. coli, P. aeruginosa, Еnterococcus faecalis and Proteus mirabilis) without
releasing PVP-I in the environment (3). Furthermore, composite Zn-alginate hydrogels released zinc
ions in addition to AC particles with adsorbed PVP-I, which induced additional microbicidal effects on
one wild yeast strain (Candida albicans). The obtained bactericidal effects were ascribed to effective
adsorption of bacteria onto AC particles and further direct contact with the adsorbed iodine, while the
antifungal activity against C. albicans was assigned to released Zn 2+.
Overall, the developed composite alginate hydrogels have shown high potentials for utilization
in variety of multifunctional wound dressings according to the specific needs.
AB  - Alginatni hidrogelovi se široko koriste u oblogama za rane zbog svoje hidrofilnosti,
biokompatibilnosti, fleksibilnosti i velikog sorpcionog kapaciteta čime obezbeđuju efikasnu regulaciju
vlažnosti rane i podstiču brzu granulaciju i reepitelizaciju oštećenog tkiva. Međutim, ove obloge nisu
bioaktivne tako da su istraživane različite metodologije kako bi se proširila funkcionalnost alginatnih
hidrogelova.
U ovom radu je prikazano nekoliko pristupa ostvarivanju tog cilja dodatkom različitih biološki
aktivnih komponenata. Ovi pristupi uključuju inkorporaciju nanočestica srebra kao potentnog
antimikrobnog agensa (1), bioaktivnih komponenata meda (2), čestica aktivnog uglja (AU) kao nosača
terapeutski aktivnih agenasa (3), kao i primenu hidrogelova Zn-alginata koji otpuštaju jone cinka.
Dobijeni kompoziti su sveobuhvatno karakterisani u pogledu sastava, citotoksičnosti, antibakterijske
aktivnosti, kinetike otpuštanja aktivnih agenasa i tretmana rana u eksperimentalnom modelu
opekotina na pacovima.
Ag/alginatni nanokompozitni hydrogelovi su usled otpuštanja jona i/ili nanočestica srebra,
pokazali izraženu baktericidnu aktivnost prema širokom spektru standardnih i kliničkih multi-
rezistentnih bakterijskih sojeva (Escherichia coli, Staphylococcus aureus, meticilin-resistentni
Staphylococcus aureus – MRSA, Acinetobacter baumannii i Pseudomonas aeruginosa). Posebno
interesantni rezultati su dobijeni u kulturama 13 kliničkih izolata A. baumannii, gde je u 6 slučajeva
postignut potpun baktericidan efekat u toku 48 h (2). Ipak, kod 3 klinička izolata nije postignuto
antibakterijsko dejstvo što ukazuje na mogućnost razvoja bakterijske rezistencije na srebro. U
tretmanu opekotina drugog stepena na pacovima, Ag/alginatni nanokompoziti su pokazali istu
efikasnost kao komercijalni medicinski proizvodi.
Kompozitni alginatni hidrogelovi sa imobilisanim česticama AU impregniranih povidon-jodom
kao model terapeutski aktivnom komponentom, su razvijeni sa ciljem da obezbede kontrolisano
otpuštanje čestica AU u rani bez otpuštanja adsorbovane supstance kako bi se na taj način postiglo
željeno dejstvo bez neželjenih efekata sistemske apsorpcije. Kompozitni Ca-alginatni hidrogelovi su
pokazali jake baktericidne efekte na dva standardna bakterijska soja i nekoliko kliničkih multi-
rezistentnih izolata iz rana (MRSA, E. coli, P. aeruginosa, Еnterococcus faecalis i Proteus mirabilis) bez
otpuštanja povidon-joda u okolinu (3). Isto tako, kompozitni Zn-alginatni hidrogelovi su otpuštali jone
cinka uz otpuštanje AU čestica sa adsorbovanim povidon-jodom što je prouzrokovalo dodatno
mikrobicidno dejstvo na jedan divlji soj gljivice Candida albicans. Dobijeni baktericidni efekti su
pripisani efikasnoj adsorpciji bakterija na čestice AU i daljem direktnom kontaktu adsorbovanog joda
sa ćelijskom membranom bakterija, dok je antifungalna aktivnost u odnosu na C. albicans pripisana
otpuštenim Zn 2+ jonima.
Može se zaključiti da su razvijeni kompozitni alginatni hdrogelovi pokazali veliki potencijal za
primenu u raznovrsnim multifunkcionalnim oblogama za rane prilagođenim specifičnim potrebama.
PB  - Savez farmaceutskih udruženja Srbije (SFUS)
C3  - Arhiv za farmaciju
T1  - Multifunctional composites based on alginate hydrogels for potential use in wound dressings
T1  - Multifunkcionalni kompoziti na bazi alginatnih hidrogelova za potencijalnu primenu u oblogama za rane
VL  - 71
IS  - 5 suplement
SP  - S22
EP  - S23
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4666
ER  - 

@conference{
author = "Obradović, Bojana and Stojkovska, Jasmina and Osmokrović, Andrea and Mišković Stanković, Vesna and Jančić, Ivan and Milenković, Marina",
year = "2021",
abstract = "Alginate hydrogels are widely used in wound dressings due to hydrophilicity, biocompatibility,
flexibility, and high sorption capacity, providing effective moisture regulation in wounds and inducing
rapid granulation and reepithelization of the damaged tissue. However, these dressings are not
bioactive so that different methodologies have been investigated to extend functionality of alginate
hydrogels.
In the present work, we show several approaches to achieve this aim by addition of different
biologically active components. These include incorporation of silver nanoparticles as potent
antimicrobial agents (1), bioactive honey components (2), activated charcoal (AC) particles as carriers
of therapeutically active agents (3) as well as the use of Zn-alginate hydrogels that release zinc ions (4).
The obtained composites were comprehensively characterized regarding composition, cytotoxicity,
antibacterial activity, release kinetics of active agents and wound treatment in a rat model.
Ag/alginate nanocomposite hydrogels releasing silver ions and/or nanoparticles exhibited high
bactericidal activity against a broad spectrum of standard and multi-drug resistant clinical bacterial
strains (Escherichia coli, Staphylococcus aureus, methicillin-resistant Staphylococcus aureus – MRSA,
Acinetobacter baumannii and Pseudomonas aeruginosa). Especially interesting results were obtained
against 13 clinical isolates of A. baumannii, which were completely extinguished over 48 h in 6 cases
(2). However, in 3 clinical isolates, antibacterial effects were not noticed implying possibility for
development of bacterial resistance to silver. In the treatment of 2nd degree burns in rats Ag/alginate
nanocomposites exhibited the same efficiency as commercial medical products (5).
Composite alginate hydrogels with immobilized AC particles impregnated with povidone iodine
(PVP-I) as a model therapeutically active agent, were developed with the aim to provide controlled
particle release in the wound without actually releasing the adsorbed substance, thus achieving the
desired activity without adverse effects by systemic absorption. The composite Ca-alginate hydrogels
induced strong bactericidal effects against two standard bacterial strains and clinical multi-resistant
wound isolates (MRSA, E. coli, P. aeruginosa, Еnterococcus faecalis and Proteus mirabilis) without
releasing PVP-I in the environment (3). Furthermore, composite Zn-alginate hydrogels released zinc
ions in addition to AC particles with adsorbed PVP-I, which induced additional microbicidal effects on
one wild yeast strain (Candida albicans). The obtained bactericidal effects were ascribed to effective
adsorption of bacteria onto AC particles and further direct contact with the adsorbed iodine, while the
antifungal activity against C. albicans was assigned to released Zn 2+.
Overall, the developed composite alginate hydrogels have shown high potentials for utilization
in variety of multifunctional wound dressings according to the specific needs., Alginatni hidrogelovi se široko koriste u oblogama za rane zbog svoje hidrofilnosti,
biokompatibilnosti, fleksibilnosti i velikog sorpcionog kapaciteta čime obezbeđuju efikasnu regulaciju
vlažnosti rane i podstiču brzu granulaciju i reepitelizaciju oštećenog tkiva. Međutim, ove obloge nisu
bioaktivne tako da su istraživane različite metodologije kako bi se proširila funkcionalnost alginatnih
hidrogelova.
U ovom radu je prikazano nekoliko pristupa ostvarivanju tog cilja dodatkom različitih biološki
aktivnih komponenata. Ovi pristupi uključuju inkorporaciju nanočestica srebra kao potentnog
antimikrobnog agensa (1), bioaktivnih komponenata meda (2), čestica aktivnog uglja (AU) kao nosača
terapeutski aktivnih agenasa (3), kao i primenu hidrogelova Zn-alginata koji otpuštaju jone cinka.
Dobijeni kompoziti su sveobuhvatno karakterisani u pogledu sastava, citotoksičnosti, antibakterijske
aktivnosti, kinetike otpuštanja aktivnih agenasa i tretmana rana u eksperimentalnom modelu
opekotina na pacovima.
Ag/alginatni nanokompozitni hydrogelovi su usled otpuštanja jona i/ili nanočestica srebra,
pokazali izraženu baktericidnu aktivnost prema širokom spektru standardnih i kliničkih multi-
rezistentnih bakterijskih sojeva (Escherichia coli, Staphylococcus aureus, meticilin-resistentni
Staphylococcus aureus – MRSA, Acinetobacter baumannii i Pseudomonas aeruginosa). Posebno
interesantni rezultati su dobijeni u kulturama 13 kliničkih izolata A. baumannii, gde je u 6 slučajeva
postignut potpun baktericidan efekat u toku 48 h (2). Ipak, kod 3 klinička izolata nije postignuto
antibakterijsko dejstvo što ukazuje na mogućnost razvoja bakterijske rezistencije na srebro. U
tretmanu opekotina drugog stepena na pacovima, Ag/alginatni nanokompoziti su pokazali istu
efikasnost kao komercijalni medicinski proizvodi.
Kompozitni alginatni hidrogelovi sa imobilisanim česticama AU impregniranih povidon-jodom
kao model terapeutski aktivnom komponentom, su razvijeni sa ciljem da obezbede kontrolisano
otpuštanje čestica AU u rani bez otpuštanja adsorbovane supstance kako bi se na taj način postiglo
željeno dejstvo bez neželjenih efekata sistemske apsorpcije. Kompozitni Ca-alginatni hidrogelovi su
pokazali jake baktericidne efekte na dva standardna bakterijska soja i nekoliko kliničkih multi-
rezistentnih izolata iz rana (MRSA, E. coli, P. aeruginosa, Еnterococcus faecalis i Proteus mirabilis) bez
otpuštanja povidon-joda u okolinu (3). Isto tako, kompozitni Zn-alginatni hidrogelovi su otpuštali jone
cinka uz otpuštanje AU čestica sa adsorbovanim povidon-jodom što je prouzrokovalo dodatno
mikrobicidno dejstvo na jedan divlji soj gljivice Candida albicans. Dobijeni baktericidni efekti su
pripisani efikasnoj adsorpciji bakterija na čestice AU i daljem direktnom kontaktu adsorbovanog joda
sa ćelijskom membranom bakterija, dok je antifungalna aktivnost u odnosu na C. albicans pripisana
otpuštenim Zn 2+ jonima.
Može se zaključiti da su razvijeni kompozitni alginatni hdrogelovi pokazali veliki potencijal za
primenu u raznovrsnim multifunkcionalnim oblogama za rane prilagođenim specifičnim potrebama.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "Multifunctional composites based on alginate hydrogels for potential use in wound dressings, Multifunkcionalni kompoziti na bazi alginatnih hidrogelova za potencijalnu primenu u oblogama za rane",
volume = "71",
number = "5 suplement",
pages = "S22-S23",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4666"
}

Obradović, B., Stojkovska, J., Osmokrović, A., Mišković Stanković, V., Jančić, I.,& Milenković, M.. (2021). Multifunctional composites based on alginate hydrogels for potential use in wound dressings. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije (SFUS)., 71(5 suplement), S22-S23.
https://hdl.handle.net/21.15107/rcub_farfar_4666

Obradović B, Stojkovska J, Osmokrović A, Mišković Stanković V, Jančić I, Milenković M. Multifunctional composites based on alginate hydrogels for potential use in wound dressings. in Arhiv za farmaciju. 2021;71(5 suplement):S22-S23.
https://hdl.handle.net/21.15107/rcub_farfar_4666 .

Obradović, Bojana, Stojkovska, Jasmina, Osmokrović, Andrea, Mišković Stanković, Vesna, Jančić, Ivan, Milenković, Marina, "Multifunctional composites based on alginate hydrogels for potential use in wound dressings" in Arhiv za farmaciju, 71, no. 5 suplement (2021):S22-S23,
https://hdl.handle.net/21.15107/rcub_farfar_4666 .

TY  - JOUR
AU  - Pavun, Leposava
AU  - Janošević-Ležaić, Aleksandra
AU  - Tanasković, Slađana
AU  - Ušjak, Dušan
AU  - Milenković, Marina
AU  - Uskoković-Marković, Snežana
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4055
AB  - The presence of residual cardiovascular disease (CVD) risk is a current dilemma in clinical practice; indeed, despite optimal management and treatment, a considerable proportion of patients still undergo major CV events. Novel lipoprotein biomarkers are suggested as possible targets for improving the outcomes of patients at higher risk for CVD, and their impact on major CV events and mortality have previously been investigated. Innovative antidiabetic therapies have recently shown a significant reduction in atherogenic lipoproteins, beyond their effects on glucose parameters; it has also been suggested that such anti-atherogenic effect may represent a valuable mechanistic explanation for the cardiovascular benefit of, at least, some of the novel antidiabetic agents, such as glucagon-like peptide-1 receptor agonists. This emphasizes the need for further research in the field in order to clearly assess the effects of innovative treatments on different novel biomarkers, including atherogenic lipoproteins, such as small dense low-density lipoprotein (LDL), lipoprotein(a) (Lp(a)) and dysfunctional high-density lipoprotein (HDL). The current article discusses the clinical importance of novel lipid biomarkers for better management of patients in order to overcome residual cardiovascular risk.
AB  - Постои постојана потреба од ефикасни лекови, комбинации на лекови и методи за спречување на бактериски и вирусни инфекции, вклучувајќи го и коронавирусот. Познато е дека улогата на елементи во траги во зајакнување на човековиот имун систем е значајна. Витамините, микроелементите, вклучувајќи цинк, железо, селен, магнезиум и бакар, масните киселини омега-3 играат значајна физиолошка улога во унапредување на имуниот систем. Цинкот е неопходен микроелемент за основните ензимски физиолошки процеси. Тој игра важна улога во делбата на клетките и е вклучен во развојот на клетки одговорни за неспецифичен имунитет. Познато е дека недостигот на цинк ги предиспонира пациентите на вирусни инфекции поради намалениот антивирусен имунитет. Од друга страна, флавоноидите како метаболити на растенијата играат важна улога во спречување на оксидативен стрес. Овој труд има за цел да ја дискутира in vitro улогата на цинкот, флавонидите и нивните комплекси, како и нивните антиоксидативни и антимикорбни активности. Разгледана е и оправданоста за истовремена употреба на цинк и флавоноиди.
PB  - Macedonian Journal of Chemistry and Chemical Engineering
T2  - Macedonian Journal of Chemistry and Chemical Engineering
T1  - Antioxidant capacity and antimicrobial effects of zinc complexes of flavonoids – does synergism exist?
T1  - Aнтиоксидативен капацитет и антимикробни ефекти на комплекси
на цинк со флавоноиди – дали постои синергизам?
VL  - 40
IS  - 2
SP  - 231
EP  - 239
DO  - 10.20450/mjcce.2021.2401
ER  - 

@article{
author = "Pavun, Leposava and Janošević-Ležaić, Aleksandra and Tanasković, Slađana and Ušjak, Dušan and Milenković, Marina and Uskoković-Marković, Snežana",
year = "2021",
abstract = "The presence of residual cardiovascular disease (CVD) risk is a current dilemma in clinical practice; indeed, despite optimal management and treatment, a considerable proportion of patients still undergo major CV events. Novel lipoprotein biomarkers are suggested as possible targets for improving the outcomes of patients at higher risk for CVD, and their impact on major CV events and mortality have previously been investigated. Innovative antidiabetic therapies have recently shown a significant reduction in atherogenic lipoproteins, beyond their effects on glucose parameters; it has also been suggested that such anti-atherogenic effect may represent a valuable mechanistic explanation for the cardiovascular benefit of, at least, some of the novel antidiabetic agents, such as glucagon-like peptide-1 receptor agonists. This emphasizes the need for further research in the field in order to clearly assess the effects of innovative treatments on different novel biomarkers, including atherogenic lipoproteins, such as small dense low-density lipoprotein (LDL), lipoprotein(a) (Lp(a)) and dysfunctional high-density lipoprotein (HDL). The current article discusses the clinical importance of novel lipid biomarkers for better management of patients in order to overcome residual cardiovascular risk., Постои постојана потреба од ефикасни лекови, комбинации на лекови и методи за спречување на бактериски и вирусни инфекции, вклучувајќи го и коронавирусот. Познато е дека улогата на елементи во траги во зајакнување на човековиот имун систем е значајна. Витамините, микроелементите, вклучувајќи цинк, железо, селен, магнезиум и бакар, масните киселини омега-3 играат значајна физиолошка улога во унапредување на имуниот систем. Цинкот е неопходен микроелемент за основните ензимски физиолошки процеси. Тој игра важна улога во делбата на клетките и е вклучен во развојот на клетки одговорни за неспецифичен имунитет. Познато е дека недостигот на цинк ги предиспонира пациентите на вирусни инфекции поради намалениот антивирусен имунитет. Од друга страна, флавоноидите како метаболити на растенијата играат важна улога во спречување на оксидативен стрес. Овој труд има за цел да ја дискутира in vitro улогата на цинкот, флавонидите и нивните комплекси, како и нивните антиоксидативни и антимикорбни активности. Разгледана е и оправданоста за истовремена употреба на цинк и флавоноиди.",
publisher = "Macedonian Journal of Chemistry and Chemical Engineering",
journal = "Macedonian Journal of Chemistry and Chemical Engineering",
title = "Antioxidant capacity and antimicrobial effects of zinc complexes of flavonoids – does synergism exist?, Aнтиоксидативен капацитет и антимикробни ефекти на комплекси
на цинк со флавоноиди – дали постои синергизам?",
volume = "40",
number = "2",
pages = "231-239",
doi = "10.20450/mjcce.2021.2401"
}

Pavun, L., Janošević-Ležaić, A., Tanasković, S., Ušjak, D., Milenković, M.,& Uskoković-Marković, S.. (2021). Antioxidant capacity and antimicrobial effects of zinc complexes of flavonoids – does synergism exist?. in Macedonian Journal of Chemistry and Chemical Engineering
Macedonian Journal of Chemistry and Chemical Engineering., 40(2), 231-239.
https://doi.org/10.20450/mjcce.2021.2401

Pavun L, Janošević-Ležaić A, Tanasković S, Ušjak D, Milenković M, Uskoković-Marković S. Antioxidant capacity and antimicrobial effects of zinc complexes of flavonoids – does synergism exist?. in Macedonian Journal of Chemistry and Chemical Engineering. 2021;40(2):231-239.
doi:10.20450/mjcce.2021.2401 .

Pavun, Leposava, Janošević-Ležaić, Aleksandra, Tanasković, Slađana, Ušjak, Dušan, Milenković, Marina, Uskoković-Marković, Snežana, "Antioxidant capacity and antimicrobial effects of zinc complexes of flavonoids – does synergism exist?" in Macedonian Journal of Chemistry and Chemical Engineering, 40, no. 2 (2021):231-239,
https://doi.org/10.20450/mjcce.2021.2401 . .

TY  - JOUR
AU  - Stevanović, Magdalena
AU  - Vukomanović, Marija
AU  - Milenković, Marina
AU  - Boccaccini, Aldo R.
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3948
AB  - Infectious diseases are a significant burden on the public health and the economic stability of societies all over the world. Drug resistance presents an ever-increasing global public health threat that involves all major microbial pathogens and antimicrobial drugs. The considerable concern regarding bacterial resistance to antimicrobial agents, bacterial adhesion, and proliferation has generated intense research in this field. Although urgently needed, an effective and long-lasting solution to this problem, i.e. microbial and especially bacterial colonization, has not yet been found. In the last decades, nanomaterials have attracted much attention because of their unique physical, chemical, and mechanical properties, due to their high surface area and size at the nanoscale. Owing to these extraordinary characteristics, nanomaterials are appropriate candidates for various applications in different fields such as medicine, pharmacy, food industry, etc. New strategies based on the use of nanomaterials such as nanoparticles, nanocapsules, nanogels, nanofibers, nanocomposites, or nanocoatings for controlling microbial virulence factors are very promising. In addition special focus recently is paid on nanostructured polymeric materials and nanocomposites with antimicrobial properties. The increasing attention within the scientific community regarding this topic can be easily assessed by searching the suitable keywords in some recognized literature databases. According to Scopus, in the last 10 years, more than 1,300 peer-reviewed documents have been published jointly containing keywords such as “nanocomposites” and “antimicrobial”. Interestingly, in the more specified search which refers to the utilization of nanostructured polymeric materials, it could be observed a similar profile i.e. growing tendency.
PB  - Frontiers Media S.A.
T2  - Frontiers in Materials
T1  - Editorial: Antimicrobial Nanostructured Polymeric Materials and Nanocomposites
VL  - 8
DO  - 10.3389/fmats.2021.748813
ER  - 

@article{
author = "Stevanović, Magdalena and Vukomanović, Marija and Milenković, Marina and Boccaccini, Aldo R.",
year = "2021",
abstract = "Infectious diseases are a significant burden on the public health and the economic stability of societies all over the world. Drug resistance presents an ever-increasing global public health threat that involves all major microbial pathogens and antimicrobial drugs. The considerable concern regarding bacterial resistance to antimicrobial agents, bacterial adhesion, and proliferation has generated intense research in this field. Although urgently needed, an effective and long-lasting solution to this problem, i.e. microbial and especially bacterial colonization, has not yet been found. In the last decades, nanomaterials have attracted much attention because of their unique physical, chemical, and mechanical properties, due to their high surface area and size at the nanoscale. Owing to these extraordinary characteristics, nanomaterials are appropriate candidates for various applications in different fields such as medicine, pharmacy, food industry, etc. New strategies based on the use of nanomaterials such as nanoparticles, nanocapsules, nanogels, nanofibers, nanocomposites, or nanocoatings for controlling microbial virulence factors are very promising. In addition special focus recently is paid on nanostructured polymeric materials and nanocomposites with antimicrobial properties. The increasing attention within the scientific community regarding this topic can be easily assessed by searching the suitable keywords in some recognized literature databases. According to Scopus, in the last 10 years, more than 1,300 peer-reviewed documents have been published jointly containing keywords such as “nanocomposites” and “antimicrobial”. Interestingly, in the more specified search which refers to the utilization of nanostructured polymeric materials, it could be observed a similar profile i.e. growing tendency.",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Materials",
title = "Editorial: Antimicrobial Nanostructured Polymeric Materials and Nanocomposites",
volume = "8",
doi = "10.3389/fmats.2021.748813"
}

Stevanović, M., Vukomanović, M., Milenković, M.,& Boccaccini, A. R.. (2021). Editorial: Antimicrobial Nanostructured Polymeric Materials and Nanocomposites. in Frontiers in Materials
Frontiers Media S.A.., 8.
https://doi.org/10.3389/fmats.2021.748813

Stevanović M, Vukomanović M, Milenković M, Boccaccini AR. Editorial: Antimicrobial Nanostructured Polymeric Materials and Nanocomposites. in Frontiers in Materials. 2021;8.
doi:10.3389/fmats.2021.748813 .

Stevanović, Magdalena, Vukomanović, Marija, Milenković, Marina, Boccaccini, Aldo R., "Editorial: Antimicrobial Nanostructured Polymeric Materials and Nanocomposites" in Frontiers in Materials, 8 (2021),
https://doi.org/10.3389/fmats.2021.748813 . .

TY  - JOUR
AU  - Awad, Mahmoud E.
AU  - López-Galindo, Alberto
AU  - Medarević, Đorđe
AU  - Milenković, Marina
AU  - Ibrić, Svetlana
AU  - El-Rahmany, Mahmoud M.
AU  - Iborra, César
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3800
AB  - The present research aims to enhance the antimicrobial activity of kaolinite surfaces by a one-step cost-effective and energy-efficient dry thermal reaction, producing an antibacterial and antifungal silver-kaolinite (Ag-Kao) nanocomposite agent. Pharmaceutical grade kaolin powder samples, with variable kaolinite structural order–disorder degree, were homogeneously mixed with silver nitrate in a proportion 1:4 AgNO3:kaolin (w/w) and sintered at 400 °C for 30 min. The composition, microstructure, microtexture and surface characteristics of the pyro-fabricated nanocomposites were characterized by XRD/XRF diffractometry, differential scanning calorimetry DSC, FT-IR spectroscopy, TEM/EDX, zeta potential (mV) measured within the 2–12 pH range, and BET method. Physicochemical stability was evaluated by silver dissociation testing under close-neutral and acidic conditions with Ag content assay using ICP-OES. The resulting Ag-Kao nanocomposites exhibited bulk silver contents ranging from 9.29% to 13.32% with high physicochemical stability in both neutral and acidic mediums (Ag dissociation rate <0.5% in 5 days). Ag nanocrystals exhibited particle sizes ranging from 5 to 30 nm, which were embedded and reinforced within the kaolinite matrix. The sizes of the Ag nanocrystals and their distribution patterns on the edges and faces of kaolinite platelets were controlled by the structural order–disorder degree. Highly ordered kaolinites (Hinckley Index, HI > 1) produced platelet edge-clustered silver nanocrystals due to the abundance of the dangling hydroxyls on platelet edges, while the highly disordered kaolinite (HI < 1) provided homogeneous platelet basal-doped silver nanocrystals due to the presence of some residual charges by exposed basal hydroxyl groups with interplatelet silver diffusivity. At pH 2, the magnitude of the positive surface charge was influenced by the silver nanocrystal size. Nanocomposites with the smallest silver nanocrystals (10–5 nm) exhibited the highest positive zeta potential (+15.2 mV to +17.0 mV), while those with larger silver nanocrystals (up to 30 nm) indicated lower positive zeta potential values (+9.5 mV to +3.6 mV). Under the same testing conditions using the Mueller-Hinton broth microdilution method, the raw kaolin samples did not show any significant antimicrobial activity, while all the pyro-fabricated Ag-Kao nanocomposite samples showed potent antibacterial and antifungal activity at low doses (MIC range 0.1–0.0125 mg/mL). Therefore, modulation of the effective electrostatic surface charge of the kaolinite platelets, via thermal doping of silver within their basal planes and edges, was found to be strongly dependent on the pH as well as the size and microtexture of the silver nanocrystals (mainly controlled by the order–disorder degree HI). The resulting modified nanostructure, with physicochemical stability and the efficient surface properties of the designed pyro-fabricated nanocomposite, led to an enhanced synergistic biophysical antimicrobial activity.
PB  - Elsevier B.V.
T2  - International Journal of Pharmaceutics
T1  - Enhanced antimicrobial activity and physicochemical stability of rapid pyro-fabricated silver-kaolinite nanocomposite
VL  - 598
DO  - 10.1016/j.ijpharm.2021.120372
ER  - 

@article{
author = "Awad, Mahmoud E. and López-Galindo, Alberto and Medarević, Đorđe and Milenković, Marina and Ibrić, Svetlana and El-Rahmany, Mahmoud M. and Iborra, César",
year = "2021",
abstract = "The present research aims to enhance the antimicrobial activity of kaolinite surfaces by a one-step cost-effective and energy-efficient dry thermal reaction, producing an antibacterial and antifungal silver-kaolinite (Ag-Kao) nanocomposite agent. Pharmaceutical grade kaolin powder samples, with variable kaolinite structural order–disorder degree, were homogeneously mixed with silver nitrate in a proportion 1:4 AgNO3:kaolin (w/w) and sintered at 400 °C for 30 min. The composition, microstructure, microtexture and surface characteristics of the pyro-fabricated nanocomposites were characterized by XRD/XRF diffractometry, differential scanning calorimetry DSC, FT-IR spectroscopy, TEM/EDX, zeta potential (mV) measured within the 2–12 pH range, and BET method. Physicochemical stability was evaluated by silver dissociation testing under close-neutral and acidic conditions with Ag content assay using ICP-OES. The resulting Ag-Kao nanocomposites exhibited bulk silver contents ranging from 9.29% to 13.32% with high physicochemical stability in both neutral and acidic mediums (Ag dissociation rate <0.5% in 5 days). Ag nanocrystals exhibited particle sizes ranging from 5 to 30 nm, which were embedded and reinforced within the kaolinite matrix. The sizes of the Ag nanocrystals and their distribution patterns on the edges and faces of kaolinite platelets were controlled by the structural order–disorder degree. Highly ordered kaolinites (Hinckley Index, HI > 1) produced platelet edge-clustered silver nanocrystals due to the abundance of the dangling hydroxyls on platelet edges, while the highly disordered kaolinite (HI < 1) provided homogeneous platelet basal-doped silver nanocrystals due to the presence of some residual charges by exposed basal hydroxyl groups with interplatelet silver diffusivity. At pH 2, the magnitude of the positive surface charge was influenced by the silver nanocrystal size. Nanocomposites with the smallest silver nanocrystals (10–5 nm) exhibited the highest positive zeta potential (+15.2 mV to +17.0 mV), while those with larger silver nanocrystals (up to 30 nm) indicated lower positive zeta potential values (+9.5 mV to +3.6 mV). Under the same testing conditions using the Mueller-Hinton broth microdilution method, the raw kaolin samples did not show any significant antimicrobial activity, while all the pyro-fabricated Ag-Kao nanocomposite samples showed potent antibacterial and antifungal activity at low doses (MIC range 0.1–0.0125 mg/mL). Therefore, modulation of the effective electrostatic surface charge of the kaolinite platelets, via thermal doping of silver within their basal planes and edges, was found to be strongly dependent on the pH as well as the size and microtexture of the silver nanocrystals (mainly controlled by the order–disorder degree HI). The resulting modified nanostructure, with physicochemical stability and the efficient surface properties of the designed pyro-fabricated nanocomposite, led to an enhanced synergistic biophysical antimicrobial activity.",
publisher = "Elsevier B.V.",
journal = "International Journal of Pharmaceutics",
title = "Enhanced antimicrobial activity and physicochemical stability of rapid pyro-fabricated silver-kaolinite nanocomposite",
volume = "598",
doi = "10.1016/j.ijpharm.2021.120372"
}

Awad, M. E., López-Galindo, A., Medarević, Đ., Milenković, M., Ibrić, S., El-Rahmany, M. M.,& Iborra, C.. (2021). Enhanced antimicrobial activity and physicochemical stability of rapid pyro-fabricated silver-kaolinite nanocomposite. in International Journal of Pharmaceutics
Elsevier B.V.., 598.
https://doi.org/10.1016/j.ijpharm.2021.120372

Awad ME, López-Galindo A, Medarević Đ, Milenković M, Ibrić S, El-Rahmany MM, Iborra C. Enhanced antimicrobial activity and physicochemical stability of rapid pyro-fabricated silver-kaolinite nanocomposite. in International Journal of Pharmaceutics. 2021;598.
doi:10.1016/j.ijpharm.2021.120372 .

Awad, Mahmoud E., López-Galindo, Alberto, Medarević, Đorđe, Milenković, Marina, Ibrić, Svetlana, El-Rahmany, Mahmoud M., Iborra, César, "Enhanced antimicrobial activity and physicochemical stability of rapid pyro-fabricated silver-kaolinite nanocomposite" in International Journal of Pharmaceutics, 598 (2021),
https://doi.org/10.1016/j.ijpharm.2021.120372 . .

TY  - JOUR
AU  - Filipović, Nenad
AU  - Ušjak, Dušan
AU  - Milenković, Marina
AU  - Zheng, Kai
AU  - Liverani, Liliana
AU  - Boccaccini, Aldo R.
AU  - Stevanović, Magdalena
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3787
AB  - Although selenium nanoparticles (SeNPs) have gained attention in the scientific community mostly through investigation of their anticancer activity, a great potential of this nanomaterial was recognized recently regarding its antimicrobial activity. The particle form, size, and surface chemistry have been recognized as crucial parameters determining the interaction of nanomaterials with biological entities. Furthermore, considering a narrow boundary between beneficial and toxic effects for selenium per se, it is clear that investigations of biomedical applications of SeNPs are very demanding and must be done with great precautions. The goal of this work is to evaluate the effects of SeNPs surface chemistry and structure on antimicrobial activity against several common bacterial strains, including Staphylococcus aureus (ATCC 6538), Enterococcus faecalis (ATCC 29212), Bacillus subtilis (ATCC 6633), and Kocuria rhizophila (ATCC 9341), as well as Escherichia coli (ATCC 8739), Salmonella Abony (NCTC 6017), Klebsiella pneumoniae (NCIMB 9111) and Pseudomonas aeruginosa (ATCC 9027), and the standard yeast strain Candida albicans (ATCC 10231). Three types of SeNPs were synthesized by chemical reduction approach using different stabilizers and reducing agents: (i) bovine serum albumin (BSA) + ascorbic acid, (ii) chitosan + ascorbic acid, and (iii) with glucose. A thorough physicochemical characterization of the obtained SeNPs was performed to determine the effects of varying synthesis parameters on their morphology, size, structure, and surface chemistry. All SeNPs were amorphous, with spherical morphology and size in the range 70–300 nm. However, the SeNPs obtained under different synthesis conditions, i.e. by using different stabilizers as well as reducing agents, exhibited different antimicrobial activity as well as cytotoxicity which are crucial for their applications. In this paper, the antimicrobial screening of the selected systems is presented, which was determined by the broth microdilution method, and inhibitory influence on the production of monomicrobial and dual-species biofilm was evaluated. The potential mechanism of action of different systems is proposed. Additionally, the cytotoxicity of SeNPs was examined on the MRC-5 cell line, in the same concentration interval as for antimicrobial testing. It was shown that formulation SeNPs-BSA expressed a significantly lower cytotoxic effect than the other two formulations.
PB  - Frontiers Media S.A.
T2  - Frontiers in Bioengineering and Biotechnology
T1  - Comparative Study of the Antimicrobial Activity of Selenium Nanoparticles With Different Surface Chemistry and Structure
VL  - 8
DO  - 10.3389/fbioe.2020.624621
ER  - 

@article{
author = "Filipović, Nenad and Ušjak, Dušan and Milenković, Marina and Zheng, Kai and Liverani, Liliana and Boccaccini, Aldo R. and Stevanović, Magdalena",
year = "2021",
abstract = "Although selenium nanoparticles (SeNPs) have gained attention in the scientific community mostly through investigation of their anticancer activity, a great potential of this nanomaterial was recognized recently regarding its antimicrobial activity. The particle form, size, and surface chemistry have been recognized as crucial parameters determining the interaction of nanomaterials with biological entities. Furthermore, considering a narrow boundary between beneficial and toxic effects for selenium per se, it is clear that investigations of biomedical applications of SeNPs are very demanding and must be done with great precautions. The goal of this work is to evaluate the effects of SeNPs surface chemistry and structure on antimicrobial activity against several common bacterial strains, including Staphylococcus aureus (ATCC 6538), Enterococcus faecalis (ATCC 29212), Bacillus subtilis (ATCC 6633), and Kocuria rhizophila (ATCC 9341), as well as Escherichia coli (ATCC 8739), Salmonella Abony (NCTC 6017), Klebsiella pneumoniae (NCIMB 9111) and Pseudomonas aeruginosa (ATCC 9027), and the standard yeast strain Candida albicans (ATCC 10231). Three types of SeNPs were synthesized by chemical reduction approach using different stabilizers and reducing agents: (i) bovine serum albumin (BSA) + ascorbic acid, (ii) chitosan + ascorbic acid, and (iii) with glucose. A thorough physicochemical characterization of the obtained SeNPs was performed to determine the effects of varying synthesis parameters on their morphology, size, structure, and surface chemistry. All SeNPs were amorphous, with spherical morphology and size in the range 70–300 nm. However, the SeNPs obtained under different synthesis conditions, i.e. by using different stabilizers as well as reducing agents, exhibited different antimicrobial activity as well as cytotoxicity which are crucial for their applications. In this paper, the antimicrobial screening of the selected systems is presented, which was determined by the broth microdilution method, and inhibitory influence on the production of monomicrobial and dual-species biofilm was evaluated. The potential mechanism of action of different systems is proposed. Additionally, the cytotoxicity of SeNPs was examined on the MRC-5 cell line, in the same concentration interval as for antimicrobial testing. It was shown that formulation SeNPs-BSA expressed a significantly lower cytotoxic effect than the other two formulations.",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Bioengineering and Biotechnology",
title = "Comparative Study of the Antimicrobial Activity of Selenium Nanoparticles With Different Surface Chemistry and Structure",
volume = "8",
doi = "10.3389/fbioe.2020.624621"
}

Filipović, N., Ušjak, D., Milenković, M., Zheng, K., Liverani, L., Boccaccini, A. R.,& Stevanović, M.. (2021). Comparative Study of the Antimicrobial Activity of Selenium Nanoparticles With Different Surface Chemistry and Structure. in Frontiers in Bioengineering and Biotechnology
Frontiers Media S.A.., 8.
https://doi.org/10.3389/fbioe.2020.624621

Filipović N, Ušjak D, Milenković M, Zheng K, Liverani L, Boccaccini AR, Stevanović M. Comparative Study of the Antimicrobial Activity of Selenium Nanoparticles With Different Surface Chemistry and Structure. in Frontiers in Bioengineering and Biotechnology. 2021;8.
doi:10.3389/fbioe.2020.624621 .

Filipović, Nenad, Ušjak, Dušan, Milenković, Marina, Zheng, Kai, Liverani, Liliana, Boccaccini, Aldo R., Stevanović, Magdalena, "Comparative Study of the Antimicrobial Activity of Selenium Nanoparticles With Different Surface Chemistry and Structure" in Frontiers in Bioengineering and Biotechnology, 8 (2021),
https://doi.org/10.3389/fbioe.2020.624621 . .

TY  - JOUR
AU  - Ušjak, Dušan
AU  - Dinić, Miroslav
AU  - Novović, Katarina
AU  - Ivković, Branka
AU  - Filipović, Nenad
AU  - Stevanović, Magdalena
AU  - Milenković, Marina
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3752
AB  - An increasing lack of available therapeutic options against Acinetobacter baumannii urged researchers to seek alternative ways to fight this extremely resistant nosocomial pathogen. Targeting its virulence appears to be a promising strategy, as it offers considerably reduced selection of resistant mutants. In this study, we tested antibiofilm potential of four synthetic chalcone derivatives against A. baumannii. Compound that showed the greatest activity was selected for further evaluation of its antivirulence properties. Real-time PCR was used to evaluate mRNA expression of biofilm-associated virulence factor genes (ompA, bap, abaI) in treated A. baumannii strains. Also, we examined virulence properties related to the expression of these genes, such as fibronectin- and collagen-mediated adhesion, surface motility, and quorum-sensing activity. The results revealed that the expression of all tested genes is downregulated together with the reduction of adhesion and motility. The conclusion is that 2′-hydroxy-2-methoxychalcone exhibits antivirulence activity against A. baumannii by inhibiting the expression of ompA and bap genes, which is reflected in reduced biofilm formation, adhesion, and surface motility.
PB  - Wiley-VCH Verlag
T2  - Chemistry and Biodiversity
T1  - Methoxy-Substituted Hydroxychalcone Reduces Biofilm Production,
Adhesion and Surface Motility of Acinetobacter baumannii by
Inhibiting ompA Gene Expression
VL  - 18
IS  - 1
DO  - 10.1002/cbdv.202000786
ER  - 

@article{
author = "Ušjak, Dušan and Dinić, Miroslav and Novović, Katarina and Ivković, Branka and Filipović, Nenad and Stevanović, Magdalena and Milenković, Marina",
year = "2021",
abstract = "An increasing lack of available therapeutic options against Acinetobacter baumannii urged researchers to seek alternative ways to fight this extremely resistant nosocomial pathogen. Targeting its virulence appears to be a promising strategy, as it offers considerably reduced selection of resistant mutants. In this study, we tested antibiofilm potential of four synthetic chalcone derivatives against A. baumannii. Compound that showed the greatest activity was selected for further evaluation of its antivirulence properties. Real-time PCR was used to evaluate mRNA expression of biofilm-associated virulence factor genes (ompA, bap, abaI) in treated A. baumannii strains. Also, we examined virulence properties related to the expression of these genes, such as fibronectin- and collagen-mediated adhesion, surface motility, and quorum-sensing activity. The results revealed that the expression of all tested genes is downregulated together with the reduction of adhesion and motility. The conclusion is that 2′-hydroxy-2-methoxychalcone exhibits antivirulence activity against A. baumannii by inhibiting the expression of ompA and bap genes, which is reflected in reduced biofilm formation, adhesion, and surface motility.",
publisher = "Wiley-VCH Verlag",
journal = "Chemistry and Biodiversity",
title = "Methoxy-Substituted Hydroxychalcone Reduces Biofilm Production,
Adhesion and Surface Motility of Acinetobacter baumannii by
Inhibiting ompA Gene Expression",
volume = "18",
number = "1",
doi = "10.1002/cbdv.202000786"
}

Ušjak, D., Dinić, M., Novović, K., Ivković, B., Filipović, N., Stevanović, M.,& Milenković, M.. (2021). Methoxy-Substituted Hydroxychalcone Reduces Biofilm Production,
Adhesion and Surface Motility of Acinetobacter baumannii by
Inhibiting ompA Gene Expression. in Chemistry and Biodiversity
Wiley-VCH Verlag., 18(1).
https://doi.org/10.1002/cbdv.202000786

Ušjak D, Dinić M, Novović K, Ivković B, Filipović N, Stevanović M, Milenković M. Methoxy-Substituted Hydroxychalcone Reduces Biofilm Production,
Adhesion and Surface Motility of Acinetobacter baumannii by
Inhibiting ompA Gene Expression. in Chemistry and Biodiversity. 2021;18(1).
doi:10.1002/cbdv.202000786 .

Ušjak, Dušan, Dinić, Miroslav, Novović, Katarina, Ivković, Branka, Filipović, Nenad, Stevanović, Magdalena, Milenković, Marina, "Methoxy-Substituted Hydroxychalcone Reduces Biofilm Production,
Adhesion and Surface Motility of Acinetobacter baumannii by
Inhibiting ompA Gene Expression" in Chemistry and Biodiversity, 18, no. 1 (2021),
https://doi.org/10.1002/cbdv.202000786 . .

TY  - JOUR
AU  - Janićijević, Dejana
AU  - Uskoković-Marković, Snežana
AU  - Ranković, Dragan
AU  - Milenković, Marina
AU  - Jevremović, Anka
AU  - Nedić-Vasiljević, Bojana
AU  - Milojević-Rakić, Maja
AU  - Bajuk-Bogdanović, Danica
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3587
AB  - Novel composites of BEA zeolite and silver tungstophosphate were prepared by different procedures: two-step impregnation, ion-exchange, and as physical mixtures with varying component mass ratios. Composites were characterized using Atomic force microscopy, Infrared, Raman and Atomic absorption spectroscopy, and results were related to adsorption properties and antimicrobial efficiencies of the composites. Prepared samples were tested as antimicrobial agents for fungal and different bacterial strains, as well as for adsorbents for pesticide nicosulfuron in aqueous solutions by using High-performance liquid chromatography. Experimental conditions for batch adsorption testing were optimized in order to efficiently eliminate nicosulfuron from aqueous solutions, while enabling antimicrobial activity of these advanced materials. Antimicrobial efficiency of composites was verified, and indicated that silver ion persistence in the solid phase is of utmost significance for the antimicrobial activity. Spectroscopic investigation revealed interaction of the silver tungstophosphate active phase and the zeolite framework, giving evidence of uniform distribution of active sites in the synthesized materials that proved to be essential for adsorption application. The best obtained adsorption capacity, as well as highest antimicrobial efficiency, is found for composite samples prepared by two-step impregnation with (BEA: silver tungstophosphate) mass ratio 2:1. The amount of nicosulfuron removed from water suspension was 38.2 mg per gram of composite, and the minimum inhibitory concentration determined for all investigated gram-negative bacteria was 125 μg mL−1.
PB  - Elsevier
T2  - Science of the Total Environment
T1  - Double active BEA zeolite/silver tungstophosphates – Antimicrobial effects and pesticide removal
VL  - 735
DO  - 10.1016/j.scitotenv.2020.139530
ER  - 

@article{
author = "Janićijević, Dejana and Uskoković-Marković, Snežana and Ranković, Dragan and Milenković, Marina and Jevremović, Anka and Nedić-Vasiljević, Bojana and Milojević-Rakić, Maja and Bajuk-Bogdanović, Danica",
year = "2020",
abstract = "Novel composites of BEA zeolite and silver tungstophosphate were prepared by different procedures: two-step impregnation, ion-exchange, and as physical mixtures with varying component mass ratios. Composites were characterized using Atomic force microscopy, Infrared, Raman and Atomic absorption spectroscopy, and results were related to adsorption properties and antimicrobial efficiencies of the composites. Prepared samples were tested as antimicrobial agents for fungal and different bacterial strains, as well as for adsorbents for pesticide nicosulfuron in aqueous solutions by using High-performance liquid chromatography. Experimental conditions for batch adsorption testing were optimized in order to efficiently eliminate nicosulfuron from aqueous solutions, while enabling antimicrobial activity of these advanced materials. Antimicrobial efficiency of composites was verified, and indicated that silver ion persistence in the solid phase is of utmost significance for the antimicrobial activity. Spectroscopic investigation revealed interaction of the silver tungstophosphate active phase and the zeolite framework, giving evidence of uniform distribution of active sites in the synthesized materials that proved to be essential for adsorption application. The best obtained adsorption capacity, as well as highest antimicrobial efficiency, is found for composite samples prepared by two-step impregnation with (BEA: silver tungstophosphate) mass ratio 2:1. The amount of nicosulfuron removed from water suspension was 38.2 mg per gram of composite, and the minimum inhibitory concentration determined for all investigated gram-negative bacteria was 125 μg mL−1.",
publisher = "Elsevier",
journal = "Science of the Total Environment",
title = "Double active BEA zeolite/silver tungstophosphates – Antimicrobial effects and pesticide removal",
volume = "735",
doi = "10.1016/j.scitotenv.2020.139530"
}

Janićijević, D., Uskoković-Marković, S., Ranković, D., Milenković, M., Jevremović, A., Nedić-Vasiljević, B., Milojević-Rakić, M.,& Bajuk-Bogdanović, D.. (2020). Double active BEA zeolite/silver tungstophosphates – Antimicrobial effects and pesticide removal. in Science of the Total Environment
Elsevier., 735.
https://doi.org/10.1016/j.scitotenv.2020.139530

Janićijević D, Uskoković-Marković S, Ranković D, Milenković M, Jevremović A, Nedić-Vasiljević B, Milojević-Rakić M, Bajuk-Bogdanović D. Double active BEA zeolite/silver tungstophosphates – Antimicrobial effects and pesticide removal. in Science of the Total Environment. 2020;735.
doi:10.1016/j.scitotenv.2020.139530 .

Janićijević, Dejana, Uskoković-Marković, Snežana, Ranković, Dragan, Milenković, Marina, Jevremović, Anka, Nedić-Vasiljević, Bojana, Milojević-Rakić, Maja, Bajuk-Bogdanović, Danica, "Double active BEA zeolite/silver tungstophosphates – Antimicrobial effects and pesticide removal" in Science of the Total Environment, 735 (2020),
https://doi.org/10.1016/j.scitotenv.2020.139530 . .

TY  - JOUR
AU  - Petrović, Snježana
AU  - Bašić, Jasmina
AU  - Mandinić, Zoran
AU  - Božić, Dragana
AU  - Milenković, Marina
AU  - Vujić, Zorica
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3579
AB  - Introduction/Objective Biofilm and pyocyanin production are essential components of Pseudomonas aeruginosa virulence and antibiotic resistance.Our objective was to examine inhibitory effect of synthetized propafenone derivatives 3-(2-Fluoro-phenyl)-1-(2- (2-hydroxy-3-propylamino-propoxy)-phenyl)-propan-1-one hydrochloride (5OF) and3-(2-Trifluoromethyl-phenyl)-1-(2-(2-hydroxy-3-propylamino-propoxy)-phenyl)-propan-1-one hydrochloride (5CF3) on biofilm and pyocyanin in Pseudomonas aeruginosa clinical strains.Methods Effects were tested on nine clinical isolates and one control laboratory strain of P. aeruginosa. In vitro analysis of biofilm growing was performed by incubating bacteria (0.5 McFarland) with 5OF and 5CF3 (500–31.2 μg/ml) and measuring optical density (OD) at 570 nm. Bacteria in medium without com-pounds were positive control. Blank medium (an uninoculated medium without test compounds) was used as negative control. Pyocyanin production was estimated by OD at 520 nm, after bacteria incubated with 5CF3 and 5OF (250 and 500 μg/ml), treated with chloroform, and chloroform layer mixed with HCl. Results A total of 500 μg/ml of 5OF and 5CF3 completely inhibited biofilm formation in 10/10 and 4/10 strains, respectively. A total of 250 μg/ml of 5OF and 5CF3 strongly inhibited biofilm formation in 7/10 strains, while inhibition with 125 μg/ml of 5OF and 5CF3 was moderate. Lower concentrations had almost no effect on biofilm production. Pyocyanin production was reduced to less than 40% of the control value in 6/9, and less than 50% of the control in 7/9 strains with 500 μg/ml of 5OF and 5CF3, respectively. At 250 μg/ml 5OF and 5CF3, most strains had pyocyanin production above 50% of the control value.Conclusion Synthetized propafenone derivatives, 5OF and 5CF3, inhibited biofilms and pyocyanin produc-tion of Pseudomonas aeruginosa clinical strains. Presented results suggest that propafenone derivatives are potential lead-compounds for synthesis of novel antipseudomonal drugs.
AB  - Увод/Циљ Производња биофилма и пиоцијанина je важан фактор вируленције и антибиотске резистенције бактерије Pseudomonas aeruginosa. Циљ рада је био да се испита инхибиторни ефекат синте-тисаних пропафенонских деривата, 3-(2-флуоро-фенил)-1-[2-(2-хидрокси-3-пропиламино-пропокси)-фенил]-про-пан-1-он-хидрохлорид) (5OF) и 3-(2-трифлуорометилфенил)-1-[2-(2-хидрокси-3-пропиламино-пропокси)-фенил]-про-пан-1-он-хидрохлорид) (5CF3), на продукцију биофилма и пиоцијанина код клиничких изолата бактерије Pseudomonas aeruginosa.Методе Ефекат пропафенонских деривата испитан је на девет клиничких изолата и једном стандардном соју бакте-рије P. aeruginosa. Утицај на продукцију биофилма испитан је in vitro, инкубацијом бактерија (0,5 по Макфарланду) са 5OF и 5CF3 (500–31,2 μg/ml), и мерењем оптичке густине на 570 nm. Бактерије у медијуму без испитиваних једињења су биле позитивна контрола, а сам медијум негативна контро-ла. Производени пиоцијанин, који је одређиван мерењем оптичке густине на 520 nm, на коинкубације бактерија са 5CF3 или 5OF (250 и 500 μg/ml), третиран је хлороформом и мешањем хлороформског слојa са HCl.Резултати При концентрацији од 500 μg/ml 5OF је довео до потпуне инхибиције продукције биофилма код свих испи-тиваних сојева (10/10). Инхибиција биофилма са 500 μg/ml5CF3 била је потпуна код 4/10 сојева. При концентрацији 5OF и 5CF3 од 250 μg/ml продукција биофилма код већине испитаних изолата била је слаба, док је при концентрацији 125 μg/ml 5OF односно 5CF3 продукција била умерена. Ниже концентрације 5OF и 5CF3 нису имале инхибиторни ефекат на формирање биофилма. У присуству 500 μg/ml 5OF у 6/10 испитиваних сојева продукција пиоцијанина пала је на мање од 40% у односу на контролну вредност. Иста концентрација (500 μg/ml) 5CF3 снизила је продукцију пиоцијанина на мање од 50% од контроле у 7/9 сојева. При концентрацији 250 μg/ml 5OF или 5CF3 већина сојева продуковала је пиоцијанин изнад 50% у односу на позитивну контролу.Закључак Синтетисани пропафенонски деривати, 5OF и 5CF3, инхибирају продукцију биофилма и пиоцијанина код клиничких сојева бактерије Pseudomonas aeruginosa. До-бијени резултати указују на то да пропафенонски деривати представљају могућа полазна једињења за синтезу нових антипсеудомонасних агенаса.
PB  - Beograd : Srpsko lekarsko društvo
T2  - Srpski Arhiv za Celokupno Lekarstvo
T1  - Inhibitory effect of propafenone derivatives on pseudomonas aeruginosa biofilm and pyocyanin production
VL  - 148
IS  - 3-4
SP  - 196
EP  - 202
DO  - 10.2298/SARH180727102P
ER  - 

@article{
author = "Petrović, Snježana and Bašić, Jasmina and Mandinić, Zoran and Božić, Dragana and Milenković, Marina and Vujić, Zorica",
year = "2020",
abstract = "Introduction/Objective Biofilm and pyocyanin production are essential components of Pseudomonas aeruginosa virulence and antibiotic resistance.Our objective was to examine inhibitory effect of synthetized propafenone derivatives 3-(2-Fluoro-phenyl)-1-(2- (2-hydroxy-3-propylamino-propoxy)-phenyl)-propan-1-one hydrochloride (5OF) and3-(2-Trifluoromethyl-phenyl)-1-(2-(2-hydroxy-3-propylamino-propoxy)-phenyl)-propan-1-one hydrochloride (5CF3) on biofilm and pyocyanin in Pseudomonas aeruginosa clinical strains.Methods Effects were tested on nine clinical isolates and one control laboratory strain of P. aeruginosa. In vitro analysis of biofilm growing was performed by incubating bacteria (0.5 McFarland) with 5OF and 5CF3 (500–31.2 μg/ml) and measuring optical density (OD) at 570 nm. Bacteria in medium without com-pounds were positive control. Blank medium (an uninoculated medium without test compounds) was used as negative control. Pyocyanin production was estimated by OD at 520 nm, after bacteria incubated with 5CF3 and 5OF (250 and 500 μg/ml), treated with chloroform, and chloroform layer mixed with HCl. Results A total of 500 μg/ml of 5OF and 5CF3 completely inhibited biofilm formation in 10/10 and 4/10 strains, respectively. A total of 250 μg/ml of 5OF and 5CF3 strongly inhibited biofilm formation in 7/10 strains, while inhibition with 125 μg/ml of 5OF and 5CF3 was moderate. Lower concentrations had almost no effect on biofilm production. Pyocyanin production was reduced to less than 40% of the control value in 6/9, and less than 50% of the control in 7/9 strains with 500 μg/ml of 5OF and 5CF3, respectively. At 250 μg/ml 5OF and 5CF3, most strains had pyocyanin production above 50% of the control value.Conclusion Synthetized propafenone derivatives, 5OF and 5CF3, inhibited biofilms and pyocyanin produc-tion of Pseudomonas aeruginosa clinical strains. Presented results suggest that propafenone derivatives are potential lead-compounds for synthesis of novel antipseudomonal drugs., Увод/Циљ Производња биофилма и пиоцијанина je важан фактор вируленције и антибиотске резистенције бактерије Pseudomonas aeruginosa. Циљ рада је био да се испита инхибиторни ефекат синте-тисаних пропафенонских деривата, 3-(2-флуоро-фенил)-1-[2-(2-хидрокси-3-пропиламино-пропокси)-фенил]-про-пан-1-он-хидрохлорид) (5OF) и 3-(2-трифлуорометилфенил)-1-[2-(2-хидрокси-3-пропиламино-пропокси)-фенил]-про-пан-1-он-хидрохлорид) (5CF3), на продукцију биофилма и пиоцијанина код клиничких изолата бактерије Pseudomonas aeruginosa.Методе Ефекат пропафенонских деривата испитан је на девет клиничких изолата и једном стандардном соју бакте-рије P. aeruginosa. Утицај на продукцију биофилма испитан је in vitro, инкубацијом бактерија (0,5 по Макфарланду) са 5OF и 5CF3 (500–31,2 μg/ml), и мерењем оптичке густине на 570 nm. Бактерије у медијуму без испитиваних једињења су биле позитивна контрола, а сам медијум негативна контро-ла. Производени пиоцијанин, који је одређиван мерењем оптичке густине на 520 nm, на коинкубације бактерија са 5CF3 или 5OF (250 и 500 μg/ml), третиран је хлороформом и мешањем хлороформског слојa са HCl.Резултати При концентрацији од 500 μg/ml 5OF је довео до потпуне инхибиције продукције биофилма код свих испи-тиваних сојева (10/10). Инхибиција биофилма са 500 μg/ml5CF3 била је потпуна код 4/10 сојева. При концентрацији 5OF и 5CF3 од 250 μg/ml продукција биофилма код већине испитаних изолата била је слаба, док је при концентрацији 125 μg/ml 5OF односно 5CF3 продукција била умерена. Ниже концентрације 5OF и 5CF3 нису имале инхибиторни ефекат на формирање биофилма. У присуству 500 μg/ml 5OF у 6/10 испитиваних сојева продукција пиоцијанина пала је на мање од 40% у односу на контролну вредност. Иста концентрација (500 μg/ml) 5CF3 снизила је продукцију пиоцијанина на мање од 50% од контроле у 7/9 сојева. При концентрацији 250 μg/ml 5OF или 5CF3 већина сојева продуковала је пиоцијанин изнад 50% у односу на позитивну контролу.Закључак Синтетисани пропафенонски деривати, 5OF и 5CF3, инхибирају продукцију биофилма и пиоцијанина код клиничких сојева бактерије Pseudomonas aeruginosa. До-бијени резултати указују на то да пропафенонски деривати представљају могућа полазна једињења за синтезу нових антипсеудомонасних агенаса.",
publisher = "Beograd : Srpsko lekarsko društvo",
journal = "Srpski Arhiv za Celokupno Lekarstvo",
title = "Inhibitory effect of propafenone derivatives on pseudomonas aeruginosa biofilm and pyocyanin production",
volume = "148",
number = "3-4",
pages = "196-202",
doi = "10.2298/SARH180727102P"
}

Petrović, S., Bašić, J., Mandinić, Z., Božić, D., Milenković, M.,& Vujić, Z.. (2020). Inhibitory effect of propafenone derivatives on pseudomonas aeruginosa biofilm and pyocyanin production. in Srpski Arhiv za Celokupno Lekarstvo
Beograd : Srpsko lekarsko društvo., 148(3-4), 196-202.
https://doi.org/10.2298/SARH180727102P

Petrović S, Bašić J, Mandinić Z, Božić D, Milenković M, Vujić Z. Inhibitory effect of propafenone derivatives on pseudomonas aeruginosa biofilm and pyocyanin production. in Srpski Arhiv za Celokupno Lekarstvo. 2020;148(3-4):196-202.
doi:10.2298/SARH180727102P .

Petrović, Snježana, Bašić, Jasmina, Mandinić, Zoran, Božić, Dragana, Milenković, Marina, Vujić, Zorica, "Inhibitory effect of propafenone derivatives on pseudomonas aeruginosa biofilm and pyocyanin production" in Srpski Arhiv za Celokupno Lekarstvo, 148, no. 3-4 (2020):196-202,
https://doi.org/10.2298/SARH180727102P . .

TY  - CONF
AU  - Ušjak, Dušan
AU  - Dinić, Miroslav
AU  - Novović, Katarina
AU  - Ivković, Branka
AU  - Filipović, Nenad
AU  - Stevanović, Magdalena
AU  - Milenković, Marina
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3758
AB  - Uvod: Acinetobacter baumannii je globalno rasprostranjen nozokomijalni patogen koji se odlikuje izuzetnom sposobnošću ekstremno brzog sticanja rezistencije na antibiotike, kao i adaptacije na preživljavanje u suvim uslovima bolničke sredine [1]. Zbog velike zastupljenosti rezistentnih sojeva protiv kojih ne postoji delotvorna terapija, Svetska zdravstvena organizacija (WHO, 2017) i Centri za kontrolu i prevenciju bolesti (CDC, 2019), označili su A. baumannii kao patogen od kritične važnosti za otkriće novih antimikrobnih agenasa ili novih terapijskih strategija [2]. Targetiranje virulencije je oblik alternativnog terapijskog pristupa koji pruža mogućnost prevencije teže kliničke slike kod inficiranih pacijenata posredstvom inhibicije ekspresije ključnih faktora virulencije, uz istovremenu redukovanu selekciju rezistentnih mutanata [3].
Rezultati i Diskusija: Od četiri različito supstituisana hidroksihalkona, sintetisanih u postupku bazno-katalizovane Claisen-Schmidt kondenzacije, selektiran je metkosi-supstituisani derivat kao najpotentniji inhibitor produkcije biofilma kod A. baumannii. Primenom Real-Time kvantitativne PCR metode sa reverznom transkriptazom ispitan je uticaj subinhibitornih koncentracija selektiranog jedinjenja (70, 35 i 10 μg/mL) na ekspresiju gena faktora virulencije povezanih sa produkcijom biofilma kod A. baumannii: ompA, bap i abaI. Pokazana je značajna dozno-zavisna nishodna ekspresija ompA gena, koji kodira OmpA protein spoljašnje membrane ćelijskog zida, koji učestvuje u brojnim virulentnim osobinama A. baumannii, kao što su adhezija, citotoksičnost, motilitet i rezistencija na imunski odgovor i antibiotike [4]. Takođe, zabeležena je značajna inhibicija ekspresije bap gena, koja je neophodna za adheziju na humane epitelne ćelije, i abaI gena, integralnog dela bakterijskog kvorum-sensing sistema, koji kodira sintazu autoinduktorskih molekula. Sposobnost antivirulentnog delovanja metoksi-supstituisanog derivata hidroksihalkona potvrđena je demonstracijom inhibicije fenotipske ekspresije faktora virulencije povezanih sa ekspresijom ompA, bap i abaI gena, kao što su adhezija za komponente ekstracelularnog matriksa (fibronektin i kolagen), površinski motilitet i produkcija autoinduktorskih molekula.
Zaključak: Metoksi-supstituisani hidroksihalkon ispoljava antivirulentno dejstvo protiv A. baumannii, pre svega posredstvom nishodne regulacije ompA gena, što se reflektuje u inhibiciji produkcije biofilma, sposobnosti adhezije i površinskog motiliteta ovog patogena.
AB  - Over the last two decades, Acinetobacter baumannii has emerged as one of the most troublesome pathogens, rapidly acquiring resistance to virtually all available antibiotics. This has urged researchers to seek alternative ways to fight this pathogen. Targeting its virulence appears to be a promising strategy, as it offers considerably reduced selection of resistant mutants. In this study, we tested antibiofilm activity of four synthetic chalcone derivatives against A. baumannii. Compound that showed the greatest activity was selected for further evaluation of its antivirulence properties. We used quantitative Real-Time PCR to evaluate mRNA expression of virulence-associated genes (ompA, bap, abaI) in extensively drug-resistant (XDR) A. baumannii wound isolate and A. baumannii ATCC 19606 strain, treated with selected compound. Also, we tested biofilm production, fibronectin- and collagen-mediated adhesion, surface motility and quorum-sensing activity of treated strains. The results revealed downregulation of the expression of all tested virulence genes together with the reduction of biofilm production, adhesion and motility. The most notable finding is significant reduction of ompA gene expression, whose encoded protein product is associated with numerous virulence traits of A. baumannii. Therefore, we conclude that selected methoxy-substituted hydroxychalcone exhibits antivirulence activity against A. baumannii by inhibiting the expression of the bacterial adhesins, most importantly OmpA, which is reflected in reduced biofilm formation, adhesion and surface motility.
C3  - FEMS Online Conference on Microbiology
T1  - Methoxy-substituted hydroxychalcone reduces biofilm production, adhesion, and surface motility of Acinetobacter baumannii by inhibiting ompA gene expression
UR  - https://hdl.handle.net/21.15107/rcub_farfar_3758
ER  - 

@conference{
author = "Ušjak, Dušan and Dinić, Miroslav and Novović, Katarina and Ivković, Branka and Filipović, Nenad and Stevanović, Magdalena and Milenković, Marina",
year = "2020",
abstract = "Uvod: Acinetobacter baumannii je globalno rasprostranjen nozokomijalni patogen koji se odlikuje izuzetnom sposobnošću ekstremno brzog sticanja rezistencije na antibiotike, kao i adaptacije na preživljavanje u suvim uslovima bolničke sredine [1]. Zbog velike zastupljenosti rezistentnih sojeva protiv kojih ne postoji delotvorna terapija, Svetska zdravstvena organizacija (WHO, 2017) i Centri za kontrolu i prevenciju bolesti (CDC, 2019), označili su A. baumannii kao patogen od kritične važnosti za otkriće novih antimikrobnih agenasa ili novih terapijskih strategija [2]. Targetiranje virulencije je oblik alternativnog terapijskog pristupa koji pruža mogućnost prevencije teže kliničke slike kod inficiranih pacijenata posredstvom inhibicije ekspresije ključnih faktora virulencije, uz istovremenu redukovanu selekciju rezistentnih mutanata [3].
Rezultati i Diskusija: Od četiri različito supstituisana hidroksihalkona, sintetisanih u postupku bazno-katalizovane Claisen-Schmidt kondenzacije, selektiran je metkosi-supstituisani derivat kao najpotentniji inhibitor produkcije biofilma kod A. baumannii. Primenom Real-Time kvantitativne PCR metode sa reverznom transkriptazom ispitan je uticaj subinhibitornih koncentracija selektiranog jedinjenja (70, 35 i 10 μg/mL) na ekspresiju gena faktora virulencije povezanih sa produkcijom biofilma kod A. baumannii: ompA, bap i abaI. Pokazana je značajna dozno-zavisna nishodna ekspresija ompA gena, koji kodira OmpA protein spoljašnje membrane ćelijskog zida, koji učestvuje u brojnim virulentnim osobinama A. baumannii, kao što su adhezija, citotoksičnost, motilitet i rezistencija na imunski odgovor i antibiotike [4]. Takođe, zabeležena je značajna inhibicija ekspresije bap gena, koja je neophodna za adheziju na humane epitelne ćelije, i abaI gena, integralnog dela bakterijskog kvorum-sensing sistema, koji kodira sintazu autoinduktorskih molekula. Sposobnost antivirulentnog delovanja metoksi-supstituisanog derivata hidroksihalkona potvrđena je demonstracijom inhibicije fenotipske ekspresije faktora virulencije povezanih sa ekspresijom ompA, bap i abaI gena, kao što su adhezija za komponente ekstracelularnog matriksa (fibronektin i kolagen), površinski motilitet i produkcija autoinduktorskih molekula.
Zaključak: Metoksi-supstituisani hidroksihalkon ispoljava antivirulentno dejstvo protiv A. baumannii, pre svega posredstvom nishodne regulacije ompA gena, što se reflektuje u inhibiciji produkcije biofilma, sposobnosti adhezije i površinskog motiliteta ovog patogena., Over the last two decades, Acinetobacter baumannii has emerged as one of the most troublesome pathogens, rapidly acquiring resistance to virtually all available antibiotics. This has urged researchers to seek alternative ways to fight this pathogen. Targeting its virulence appears to be a promising strategy, as it offers considerably reduced selection of resistant mutants. In this study, we tested antibiofilm activity of four synthetic chalcone derivatives against A. baumannii. Compound that showed the greatest activity was selected for further evaluation of its antivirulence properties. We used quantitative Real-Time PCR to evaluate mRNA expression of virulence-associated genes (ompA, bap, abaI) in extensively drug-resistant (XDR) A. baumannii wound isolate and A. baumannii ATCC 19606 strain, treated with selected compound. Also, we tested biofilm production, fibronectin- and collagen-mediated adhesion, surface motility and quorum-sensing activity of treated strains. The results revealed downregulation of the expression of all tested virulence genes together with the reduction of biofilm production, adhesion and motility. The most notable finding is significant reduction of ompA gene expression, whose encoded protein product is associated with numerous virulence traits of A. baumannii. Therefore, we conclude that selected methoxy-substituted hydroxychalcone exhibits antivirulence activity against A. baumannii by inhibiting the expression of the bacterial adhesins, most importantly OmpA, which is reflected in reduced biofilm formation, adhesion and surface motility.",
journal = "FEMS Online Conference on Microbiology",
title = "Methoxy-substituted hydroxychalcone reduces biofilm production, adhesion, and surface motility of Acinetobacter baumannii by inhibiting ompA gene expression",
url = "https://hdl.handle.net/21.15107/rcub_farfar_3758"
}

Ušjak, D., Dinić, M., Novović, K., Ivković, B., Filipović, N., Stevanović, M.,& Milenković, M.. (2020). Methoxy-substituted hydroxychalcone reduces biofilm production, adhesion, and surface motility of Acinetobacter baumannii by inhibiting ompA gene expression. in FEMS Online Conference on Microbiology.
https://hdl.handle.net/21.15107/rcub_farfar_3758

Ušjak D, Dinić M, Novović K, Ivković B, Filipović N, Stevanović M, Milenković M. Methoxy-substituted hydroxychalcone reduces biofilm production, adhesion, and surface motility of Acinetobacter baumannii by inhibiting ompA gene expression. in FEMS Online Conference on Microbiology. 2020;.
https://hdl.handle.net/21.15107/rcub_farfar_3758 .

Ušjak, Dušan, Dinić, Miroslav, Novović, Katarina, Ivković, Branka, Filipović, Nenad, Stevanović, Magdalena, Milenković, Marina, "Methoxy-substituted hydroxychalcone reduces biofilm production, adhesion, and surface motility of Acinetobacter baumannii by inhibiting ompA gene expression" in FEMS Online Conference on Microbiology (2020),
https://hdl.handle.net/21.15107/rcub_farfar_3758 .

TY  - JOUR
AU  - Marčetić, Mirjana
AU  - Božić, Dragana
AU  - Milenković, Marina
AU  - Kovačević, Mirjana
AU  - Kovačević, Nada
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3664
AB  - Ispitivano je delovanje etarskih ulja korena, herbe i ploda devesilja, Seseli rigidum iz dve prirodne populacije na 28 izolata Candida  albicans  i jedan standardni soj C.  albicans  ATCC 10259. Sojevi C. albicans koji su korišćeni u ovom ispitivanju izolovani su iz orofaringealnog brisa, vaginalnog i/ili cervikalnog brisa, brisa oka, brisa rane, fecesa i urina.  Ispitivani  sojevi  su  pokazali  visok  stepen  rezistencije  prema  delovanju  kontrolnog antimikotika  flukonazola,  a  u  nešto  manjoj  meri  i  amfotericina B.  Etarska  ulja  devesilja, primenjena u koncentracijama 50-200 μg/ml, uzrokovala su inhibiciju rasta ispitivanih sojeva (28,6-46,4% testiranih sojeva). Inhibitorni efekat na tri soja C. albicans ostvaren je sa etarskim uljem korena, sa oba lokaliteta, već pri koncentraciji od 50 μg/ml. Dominantno jedinjenje u ovim etarskim uljima je predstavljao falkarinol (57,5% i 88,8%). Slabiju aktivnost su ispoljila etarska  ulje  herbe  i  ploda  devesilja,  koja  su  u  koncentraciji  od  100  μg/ml  inhibirala  rast pojedinih  ispitivanih  sojeva.  Kod  etarskog  ulja  ploda  uočeno  je  nešto  izraženije  delovanje etarskog  ulja  populacije  iz  Brđanske  klisure,  koje  je  sadržalo veću  količinu  α-pinena  i                         β-felandrena, a manju količinu sabinena.  Dobijeni rezultati su pokazali da etarska ulja korena, herbe i ploda devesilja ispoljavaju značajnu antifungalnu aktivnost na pojedine rezistentne sojeve C. albicans.
AB  - The antimicrobial activity of the essential oils of root, aerial parts and fruit from two natural populations of Seseli rigidum was tested against 28 isolates of Candida albicans and one laboratory control strain of C. albicans ATCC 10259. Strains of C. albicans were isolated from oropharyngeal swabs, vaginal and/or cervical swabs, eye swabs, wound swabs, feces and urine. The investigated strains showed high level of resistance towards the activity of control antimycotic agent fluconazole and to a lower extent towards amphotericin B. The S. rigidum essential oils, in the concentrations of 50-200 μg/ml, inhibited the growth of investigated strains (28.6-46.4% of tested strains). The essential oils of root from both localities showed antifungal effect at the concentration 50 μg/ml. The dominant compound in the root essential oils was falcarinol (57.5% and 88.8%). The lower activity was obtained with the essential oil of aerial parts or fruit. The 100 μg/ml of essential oil of aerial parts showed significant effect on C. albicans strains. The essential oil of fruit from population from Brdjanska gorge exerted somehow higher effect. It was characterised by higher α-pinene and β-phellandrene content and lower amount of sabinene. The obtained results revealed the significant antifungal effects of S. rigidum root, aerial parts and fruit essential oils against resistant strains of C. albicans.
PB  - Pharmaceutical Association of Serbia
T2  - Arhiv za farmaciju
T1  - Antifungal activity of the esssential oil of seseli rigidum waldst. & kit. (apiaceae) on the growth of isolates of candida albicans
T1  - Antifungalna aktivnost etarskog ulja seseli rigidum waldst. & kit. (apiaceae) na rast izolata candida albicans
VL  - 69
IS  - 2
SP  - 67
EP  - 79
DO  - 10.5937/arhfarm1902067x
ER  - 

@article{
author = "Marčetić, Mirjana and Božić, Dragana and Milenković, Marina and Kovačević, Mirjana and Kovačević, Nada",
year = "2019",
abstract = "Ispitivano je delovanje etarskih ulja korena, herbe i ploda devesilja, Seseli rigidum iz dve prirodne populacije na 28 izolata Candida  albicans  i jedan standardni soj C.  albicans  ATCC 10259. Sojevi C. albicans koji su korišćeni u ovom ispitivanju izolovani su iz orofaringealnog brisa, vaginalnog i/ili cervikalnog brisa, brisa oka, brisa rane, fecesa i urina.  Ispitivani  sojevi  su  pokazali  visok  stepen  rezistencije  prema  delovanju  kontrolnog antimikotika  flukonazola,  a  u  nešto  manjoj  meri  i  amfotericina B.  Etarska  ulja  devesilja, primenjena u koncentracijama 50-200 μg/ml, uzrokovala su inhibiciju rasta ispitivanih sojeva (28,6-46,4% testiranih sojeva). Inhibitorni efekat na tri soja C. albicans ostvaren je sa etarskim uljem korena, sa oba lokaliteta, već pri koncentraciji od 50 μg/ml. Dominantno jedinjenje u ovim etarskim uljima je predstavljao falkarinol (57,5% i 88,8%). Slabiju aktivnost su ispoljila etarska  ulje  herbe  i  ploda  devesilja,  koja  su  u  koncentraciji  od  100  μg/ml  inhibirala  rast pojedinih  ispitivanih  sojeva.  Kod  etarskog  ulja  ploda  uočeno  je  nešto  izraženije  delovanje etarskog  ulja  populacije  iz  Brđanske  klisure,  koje  je  sadržalo veću  količinu  α-pinena  i                         β-felandrena, a manju količinu sabinena.  Dobijeni rezultati su pokazali da etarska ulja korena, herbe i ploda devesilja ispoljavaju značajnu antifungalnu aktivnost na pojedine rezistentne sojeve C. albicans., The antimicrobial activity of the essential oils of root, aerial parts and fruit from two natural populations of Seseli rigidum was tested against 28 isolates of Candida albicans and one laboratory control strain of C. albicans ATCC 10259. Strains of C. albicans were isolated from oropharyngeal swabs, vaginal and/or cervical swabs, eye swabs, wound swabs, feces and urine. The investigated strains showed high level of resistance towards the activity of control antimycotic agent fluconazole and to a lower extent towards amphotericin B. The S. rigidum essential oils, in the concentrations of 50-200 μg/ml, inhibited the growth of investigated strains (28.6-46.4% of tested strains). The essential oils of root from both localities showed antifungal effect at the concentration 50 μg/ml. The dominant compound in the root essential oils was falcarinol (57.5% and 88.8%). The lower activity was obtained with the essential oil of aerial parts or fruit. The 100 μg/ml of essential oil of aerial parts showed significant effect on C. albicans strains. The essential oil of fruit from population from Brdjanska gorge exerted somehow higher effect. It was characterised by higher α-pinene and β-phellandrene content and lower amount of sabinene. The obtained results revealed the significant antifungal effects of S. rigidum root, aerial parts and fruit essential oils against resistant strains of C. albicans.",
publisher = "Pharmaceutical Association of Serbia",
journal = "Arhiv za farmaciju",
title = "Antifungal activity of the esssential oil of seseli rigidum waldst. & kit. (apiaceae) on the growth of isolates of candida albicans, Antifungalna aktivnost etarskog ulja seseli rigidum waldst. & kit. (apiaceae) na rast izolata candida albicans",
volume = "69",
number = "2",
pages = "67-79",
doi = "10.5937/arhfarm1902067x"
}

Marčetić, M., Božić, D., Milenković, M., Kovačević, M.,& Kovačević, N.. (2019). Antifungal activity of the esssential oil of seseli rigidum waldst. & kit. (apiaceae) on the growth of isolates of candida albicans. in Arhiv za farmaciju
Pharmaceutical Association of Serbia., 69(2), 67-79.
https://doi.org/10.5937/arhfarm1902067x

Marčetić M, Božić D, Milenković M, Kovačević M, Kovačević N. Antifungal activity of the esssential oil of seseli rigidum waldst. & kit. (apiaceae) on the growth of isolates of candida albicans. in Arhiv za farmaciju. 2019;69(2):67-79.
doi:10.5937/arhfarm1902067x .

Marčetić, Mirjana, Božić, Dragana, Milenković, Marina, Kovačević, Mirjana, Kovačević, Nada, "Antifungal activity of the esssential oil of seseli rigidum waldst. & kit. (apiaceae) on the growth of isolates of candida albicans" in Arhiv za farmaciju, 69, no. 2 (2019):67-79,
https://doi.org/10.5937/arhfarm1902067x . .

TY  - JOUR
AU  - Usjak, Dušan
AU  - Ivković, Branka
AU  - Božić, Dragana
AU  - Bošković, Lidija
AU  - Milenković, Marina
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3351
AB  - Acinetobacter baumannii and Pseudomonas aeruginosa are frequent multiresistant nosocomial pathogens that cause wound and pulmonary infections in hospitalized patients. As being increasingly resistant to most clinically available antibiotics, there is a constant need for exploration of new substances that could kill them or inhibit their growth, or alternatively inhibit some of their essential virulence factors. Chalcones are chemical compounds with well-documented antimicrobial potential. The aim of this study was to examine effectiveness of four newly-synthesized chalcones against the multiresistant clinical strains of A. baumannii and P. aeruginosa. Antibacterial activity of chalcones was investigated with broth-microdilution test and time-dependent killing assay. Synergistic effects of tested compounds with antibiotics (meropenem, amikacin and ciprofloxacin) were determined by checkerboard assay. The effects of chalcones on expression of virulence factors in P. aeruginosa (pyocyanin production, swimming and swarming motility) and A. baumannii (twitching and surface-associated motility), along with their biofilm production, were also examined. The obtained results indicate substantial antimicrobial activity of the tested chalcones (MICs = 100-175 mu g/mL) and several synergistic interactions with antibiotics, as well as notable reduction in expression of all investigated virulence factors. These promising results may constitute a good basis for further research.
PB  - Academic Press Ltd- Elsevier Science Ltd, London
T2  - Microbial Pathogenesis
T1  - Antimicrobial activity of novel chalcones and modulation of virulence factors in hospital strains of Acinetobacter baumannii and Pseudomonas aeruginosa
VL  - 131
SP  - 186
EP  - 196
DO  - 10.1016/j.micpath.2019.04.015
ER  - 

@article{
author = "Usjak, Dušan and Ivković, Branka and Božić, Dragana and Bošković, Lidija and Milenković, Marina",
year = "2019",
abstract = "Acinetobacter baumannii and Pseudomonas aeruginosa are frequent multiresistant nosocomial pathogens that cause wound and pulmonary infections in hospitalized patients. As being increasingly resistant to most clinically available antibiotics, there is a constant need for exploration of new substances that could kill them or inhibit their growth, or alternatively inhibit some of their essential virulence factors. Chalcones are chemical compounds with well-documented antimicrobial potential. The aim of this study was to examine effectiveness of four newly-synthesized chalcones against the multiresistant clinical strains of A. baumannii and P. aeruginosa. Antibacterial activity of chalcones was investigated with broth-microdilution test and time-dependent killing assay. Synergistic effects of tested compounds with antibiotics (meropenem, amikacin and ciprofloxacin) were determined by checkerboard assay. The effects of chalcones on expression of virulence factors in P. aeruginosa (pyocyanin production, swimming and swarming motility) and A. baumannii (twitching and surface-associated motility), along with their biofilm production, were also examined. The obtained results indicate substantial antimicrobial activity of the tested chalcones (MICs = 100-175 mu g/mL) and several synergistic interactions with antibiotics, as well as notable reduction in expression of all investigated virulence factors. These promising results may constitute a good basis for further research.",
publisher = "Academic Press Ltd- Elsevier Science Ltd, London",
journal = "Microbial Pathogenesis",
title = "Antimicrobial activity of novel chalcones and modulation of virulence factors in hospital strains of Acinetobacter baumannii and Pseudomonas aeruginosa",
volume = "131",
pages = "186-196",
doi = "10.1016/j.micpath.2019.04.015"
}

Usjak, D., Ivković, B., Božić, D., Bošković, L.,& Milenković, M.. (2019). Antimicrobial activity of novel chalcones and modulation of virulence factors in hospital strains of Acinetobacter baumannii and Pseudomonas aeruginosa. in Microbial Pathogenesis
Academic Press Ltd- Elsevier Science Ltd, London., 131, 186-196.
https://doi.org/10.1016/j.micpath.2019.04.015

Usjak D, Ivković B, Božić D, Bošković L, Milenković M. Antimicrobial activity of novel chalcones and modulation of virulence factors in hospital strains of Acinetobacter baumannii and Pseudomonas aeruginosa. in Microbial Pathogenesis. 2019;131:186-196.
doi:10.1016/j.micpath.2019.04.015 .

Usjak, Dušan, Ivković, Branka, Božić, Dragana, Bošković, Lidija, Milenković, Marina, "Antimicrobial activity of novel chalcones and modulation of virulence factors in hospital strains of Acinetobacter baumannii and Pseudomonas aeruginosa" in Microbial Pathogenesis, 131 (2019):186-196,
https://doi.org/10.1016/j.micpath.2019.04.015 . .

TY  - JOUR
AU  - Stanisavljević, Dragana
AU  - Đorđević, Sofija
AU  - Milenković, Marina
AU  - Zlatković, Branislav
AU  - Nikolova, Milena T.
AU  - Veličković, Dragan
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3312
AB  - Isolates of medicinal and aromatic herbs are used as additives in non-alcoholic drinks to improve the sensory characteristics and antioxidant potential. The method of drying the plant material, among other factors, has a profound influence on the chemical composition and pharmacological activities of plant extracts. This paper analyzes the effect of the drying technique (I - natural drying, II - in the laboratory oven, III - in low-temperature condensational drier) on the volatile fraction of the wild mint (Mentha longifolia (L.) Hudson) extract, in order to select an extract of the best quality for preparation of non-alcoholic drinks. The volatile profile of the extracts was determined by the GC-FID and GC-MS techniques, the antimicrobial activity by the microdilution technique, the antioxidant activity by the DPPH and FRAP assays, and the sensory acceptance according to the Regulation on quality for refreshing non-alcoholic beverages. The results showed that piperitone was the major component of the volatile fraction extract of the natural drying herb and low-temperature condensational drier herb (53.9% and 61.1%, respectively), while the extract of laboratory oven herb was rich in menthone (35.5%). At the concentrations in the range of 0.8-3.2 mg/mL the extracts better inhibited the Gram (+) bacteria. The beverage to which the extract of naturally dried wild mint was added, showed the antioxidant activity (9.09 +/- 0.17 mu mol Fe2+/mL by FRAP and 14.00 +/- 3.00 mu L/mL by DPPH method) and good sensorial characteristics (concentration of the extract 0.8 g/L).
PB  - Mattioli 1885, Fidenza
T2  - Psychiatry Research
T1  - Wild mint (Mentha longifolia) extracts in the production of non-alcoholic beverages
VL  - 21
IS  - 1
SP  - 202
EP  - 209
DO  - 10.23751/pn.v21i1.7256
ER  - 

@article{
author = "Stanisavljević, Dragana and Đorđević, Sofija and Milenković, Marina and Zlatković, Branislav and Nikolova, Milena T. and Veličković, Dragan",
year = "2019",
abstract = "Isolates of medicinal and aromatic herbs are used as additives in non-alcoholic drinks to improve the sensory characteristics and antioxidant potential. The method of drying the plant material, among other factors, has a profound influence on the chemical composition and pharmacological activities of plant extracts. This paper analyzes the effect of the drying technique (I - natural drying, II - in the laboratory oven, III - in low-temperature condensational drier) on the volatile fraction of the wild mint (Mentha longifolia (L.) Hudson) extract, in order to select an extract of the best quality for preparation of non-alcoholic drinks. The volatile profile of the extracts was determined by the GC-FID and GC-MS techniques, the antimicrobial activity by the microdilution technique, the antioxidant activity by the DPPH and FRAP assays, and the sensory acceptance according to the Regulation on quality for refreshing non-alcoholic beverages. The results showed that piperitone was the major component of the volatile fraction extract of the natural drying herb and low-temperature condensational drier herb (53.9% and 61.1%, respectively), while the extract of laboratory oven herb was rich in menthone (35.5%). At the concentrations in the range of 0.8-3.2 mg/mL the extracts better inhibited the Gram (+) bacteria. The beverage to which the extract of naturally dried wild mint was added, showed the antioxidant activity (9.09 +/- 0.17 mu mol Fe2+/mL by FRAP and 14.00 +/- 3.00 mu L/mL by DPPH method) and good sensorial characteristics (concentration of the extract 0.8 g/L).",
publisher = "Mattioli 1885, Fidenza",
journal = "Psychiatry Research",
title = "Wild mint (Mentha longifolia) extracts in the production of non-alcoholic beverages",
volume = "21",
number = "1",
pages = "202-209",
doi = "10.23751/pn.v21i1.7256"
}

Stanisavljević, D., Đorđević, S., Milenković, M., Zlatković, B., Nikolova, M. T.,& Veličković, D.. (2019). Wild mint (Mentha longifolia) extracts in the production of non-alcoholic beverages. in Psychiatry Research
Mattioli 1885, Fidenza., 21(1), 202-209.
https://doi.org/10.23751/pn.v21i1.7256

Stanisavljević D, Đorđević S, Milenković M, Zlatković B, Nikolova MT, Veličković D. Wild mint (Mentha longifolia) extracts in the production of non-alcoholic beverages. in Psychiatry Research. 2019;21(1):202-209.
doi:10.23751/pn.v21i1.7256 .

Stanisavljević, Dragana, Đorđević, Sofija, Milenković, Marina, Zlatković, Branislav, Nikolova, Milena T., Veličković, Dragan, "Wild mint (Mentha longifolia) extracts in the production of non-alcoholic beverages" in Psychiatry Research, 21, no. 1 (2019):202-209,
https://doi.org/10.23751/pn.v21i1.7256 . .