TY  - JOUR
AU  - Tosić, Svetlana
AU  - Stojicić, Dragana
AU  - Slavkovska, Violeta
AU  - Mihailov-Krstev, Tatjana
AU  - Zlatković, Bojan
AU  - Budimir, Snežana
AU  - Uzelac, Branka
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3363
AB  - Main conclusion In vitro culture conditions and kinetin induced quantitative modifications in the production of the major volatile constituents in Micromeria croatica plantlets. Antimicrobial activity of methanolic extracts obtained from micropropagated and wild-growing plants was evaluated. Micromeria spp. are aromatic plants, many of which were shown to exhibit various biological effects. The present study aimed to determine the content and the composition of the essential oil of in vitro-cultured Micromeria croatica (Pers.) Schott and to evaluate the in vitro antimicrobial activity of its methanolic extract, in order to compare its phytochemical profile and biological activity with wild-growing plants. Shoots regenerated on MS medium without plant growth regulators (PGRs) or supplemented with kinetin were used for phytochemical analysis. Essential oils from both native plant material and in vitro-cultivated M. croatica plants, with a total of 44 identified constituents, were dominated by oxygenated monoterpenes. Borneol was the main component detected in wild-growing plants (25.28%) and micropropagated plants grown on PGR-free medium (20.30%). Kinetin treatment led to increased oil yield and favored the production of oxygenated monoterpenes, dominated by geranial (33.53%) and cis-p-mentha-1(7),8-dien-ol (23.69%). The percentage of total sesquiterpenoids in micropropagated plant material was considerably lower than in wild-growing plants. In vitro culture conditions and PGRs affected the production of essential oils, inducing quantitative modifications in the production of the major volatile constituents in M. croatica plantlets. The antimicrobial activity of M. croatica methanolic extracts was investigated using the broth microdilution method. Extracts obtained from in vitro cultures generally exhibited greater antibacterial potential, compared to wild-growing plants. Among six bacterial strains tested, Bacillus cereus and Staphylococcus aureus were the most sensitive microorganisms. The present study provided evidence that in vitro culture conditions might favorably affect the antimicrobial activity of M. croatica methanolic extracts.
PB  - Springer, New York
T2  - Planta
T1  - Phytochemical composition and biological activities of native and in vitro-propagated Micromeria croatica (Pers.) Schott (Lamiaceae)
VL  - 249
IS  - 5
SP  - 1365
EP  - 1377
DO  - 10.1007/s00425-018-03071-5
ER  - 

@article{
author = "Tosić, Svetlana and Stojicić, Dragana and Slavkovska, Violeta and Mihailov-Krstev, Tatjana and Zlatković, Bojan and Budimir, Snežana and Uzelac, Branka",
year = "2019",
abstract = "Main conclusion In vitro culture conditions and kinetin induced quantitative modifications in the production of the major volatile constituents in Micromeria croatica plantlets. Antimicrobial activity of methanolic extracts obtained from micropropagated and wild-growing plants was evaluated. Micromeria spp. are aromatic plants, many of which were shown to exhibit various biological effects. The present study aimed to determine the content and the composition of the essential oil of in vitro-cultured Micromeria croatica (Pers.) Schott and to evaluate the in vitro antimicrobial activity of its methanolic extract, in order to compare its phytochemical profile and biological activity with wild-growing plants. Shoots regenerated on MS medium without plant growth regulators (PGRs) or supplemented with kinetin were used for phytochemical analysis. Essential oils from both native plant material and in vitro-cultivated M. croatica plants, with a total of 44 identified constituents, were dominated by oxygenated monoterpenes. Borneol was the main component detected in wild-growing plants (25.28%) and micropropagated plants grown on PGR-free medium (20.30%). Kinetin treatment led to increased oil yield and favored the production of oxygenated monoterpenes, dominated by geranial (33.53%) and cis-p-mentha-1(7),8-dien-ol (23.69%). The percentage of total sesquiterpenoids in micropropagated plant material was considerably lower than in wild-growing plants. In vitro culture conditions and PGRs affected the production of essential oils, inducing quantitative modifications in the production of the major volatile constituents in M. croatica plantlets. The antimicrobial activity of M. croatica methanolic extracts was investigated using the broth microdilution method. Extracts obtained from in vitro cultures generally exhibited greater antibacterial potential, compared to wild-growing plants. Among six bacterial strains tested, Bacillus cereus and Staphylococcus aureus were the most sensitive microorganisms. The present study provided evidence that in vitro culture conditions might favorably affect the antimicrobial activity of M. croatica methanolic extracts.",
publisher = "Springer, New York",
journal = "Planta",
title = "Phytochemical composition and biological activities of native and in vitro-propagated Micromeria croatica (Pers.) Schott (Lamiaceae)",
volume = "249",
number = "5",
pages = "1365-1377",
doi = "10.1007/s00425-018-03071-5"
}

Tosić, S., Stojicić, D., Slavkovska, V., Mihailov-Krstev, T., Zlatković, B., Budimir, S.,& Uzelac, B.. (2019). Phytochemical composition and biological activities of native and in vitro-propagated Micromeria croatica (Pers.) Schott (Lamiaceae). in Planta
Springer, New York., 249(5), 1365-1377.
https://doi.org/10.1007/s00425-018-03071-5

Tosić S, Stojicić D, Slavkovska V, Mihailov-Krstev T, Zlatković B, Budimir S, Uzelac B. Phytochemical composition and biological activities of native and in vitro-propagated Micromeria croatica (Pers.) Schott (Lamiaceae). in Planta. 2019;249(5):1365-1377.
doi:10.1007/s00425-018-03071-5 .

Tosić, Svetlana, Stojicić, Dragana, Slavkovska, Violeta, Mihailov-Krstev, Tatjana, Zlatković, Bojan, Budimir, Snežana, Uzelac, Branka, "Phytochemical composition and biological activities of native and in vitro-propagated Micromeria croatica (Pers.) Schott (Lamiaceae)" in Planta, 249, no. 5 (2019):1365-1377,
https://doi.org/10.1007/s00425-018-03071-5 . .

TY  - JOUR
AU  - Stojicić, Dragana
AU  - Tosić, Svetlana
AU  - Slavkovska, Violeta
AU  - Zlatković, Bojan
AU  - Budimir, Snežana
AU  - Janošević, Dušica
AU  - Uzelac, Branka
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2642
AB  - In vitro conditions and benzyladenine influenced both content and composition of micropropagated Micromeria pulegium essential oils, with pulegone and menthone being the main essential oil components. The content and chemical composition of Micromeria pulegium (Rochel) Benth. essential oils were studied in native plant material at vegetative stage and in micropropagated plants, obtained from nodal segments cultured on solid MS medium supplemented with N-6-benzyladenine (BA) or kinetin at different concentrations, alone or in combination with indole-3-acetic acid. Shoot proliferation was achieved in all treatments, but the highest biomass production was obtained after treatment with 10 mu M BA. Phytochemical analysis identified up to 21 compounds in the essential oils of wild-growing and in vitro cultivated plants, both showing very high percentages of total monoterpenoids dominated by oxygenated monoterpenes of the menthane type. Pulegone and menthone were the main essential oil components detected in both wild-growing plants (60.07 and 26.85 %, respectively) and micropropagated plants grown on either plant growth regulator-free medium (44.57 and 29.14 %, respectively) or BA-supplemented medium (50.77 and 14.45 %, respectively). The percentage of total sesquiterpenoids increased in vitro, particularly owing to sesquiterpene hydrocarbons that were not found in wild-growing plants. Differences in both content and the composition of the essential oils obtained from different samples indicated that in vitro culture conditions and plant growth regulators significantly influence the essential oils properties. In addition, the morphology and structure of M. pulegium glandular trichomes in relation to the secretory process were characterized for the first time using SEM and light microscopy, and their secretion was histochemically analyzed.
PB  - Springer, New York
T2  - Planta
T1  - Glandular trichomes and essential oil characteristics of in vitro propagated Micromeria pulegium (Rochel) Benth. (Lamiaceae)
VL  - 244
IS  - 2
SP  - 393
EP  - 404
DO  - 10.1007/s00425-016-2513-7
ER  - 

@article{
author = "Stojicić, Dragana and Tosić, Svetlana and Slavkovska, Violeta and Zlatković, Bojan and Budimir, Snežana and Janošević, Dušica and Uzelac, Branka",
year = "2016",
abstract = "In vitro conditions and benzyladenine influenced both content and composition of micropropagated Micromeria pulegium essential oils, with pulegone and menthone being the main essential oil components. The content and chemical composition of Micromeria pulegium (Rochel) Benth. essential oils were studied in native plant material at vegetative stage and in micropropagated plants, obtained from nodal segments cultured on solid MS medium supplemented with N-6-benzyladenine (BA) or kinetin at different concentrations, alone or in combination with indole-3-acetic acid. Shoot proliferation was achieved in all treatments, but the highest biomass production was obtained after treatment with 10 mu M BA. Phytochemical analysis identified up to 21 compounds in the essential oils of wild-growing and in vitro cultivated plants, both showing very high percentages of total monoterpenoids dominated by oxygenated monoterpenes of the menthane type. Pulegone and menthone were the main essential oil components detected in both wild-growing plants (60.07 and 26.85 %, respectively) and micropropagated plants grown on either plant growth regulator-free medium (44.57 and 29.14 %, respectively) or BA-supplemented medium (50.77 and 14.45 %, respectively). The percentage of total sesquiterpenoids increased in vitro, particularly owing to sesquiterpene hydrocarbons that were not found in wild-growing plants. Differences in both content and the composition of the essential oils obtained from different samples indicated that in vitro culture conditions and plant growth regulators significantly influence the essential oils properties. In addition, the morphology and structure of M. pulegium glandular trichomes in relation to the secretory process were characterized for the first time using SEM and light microscopy, and their secretion was histochemically analyzed.",
publisher = "Springer, New York",
journal = "Planta",
title = "Glandular trichomes and essential oil characteristics of in vitro propagated Micromeria pulegium (Rochel) Benth. (Lamiaceae)",
volume = "244",
number = "2",
pages = "393-404",
doi = "10.1007/s00425-016-2513-7"
}

Stojicić, D., Tosić, S., Slavkovska, V., Zlatković, B., Budimir, S., Janošević, D.,& Uzelac, B.. (2016). Glandular trichomes and essential oil characteristics of in vitro propagated Micromeria pulegium (Rochel) Benth. (Lamiaceae). in Planta
Springer, New York., 244(2), 393-404.
https://doi.org/10.1007/s00425-016-2513-7

Stojicić D, Tosić S, Slavkovska V, Zlatković B, Budimir S, Janošević D, Uzelac B. Glandular trichomes and essential oil characteristics of in vitro propagated Micromeria pulegium (Rochel) Benth. (Lamiaceae). in Planta. 2016;244(2):393-404.
doi:10.1007/s00425-016-2513-7 .

Stojicić, Dragana, Tosić, Svetlana, Slavkovska, Violeta, Zlatković, Bojan, Budimir, Snežana, Janošević, Dušica, Uzelac, Branka, "Glandular trichomes and essential oil characteristics of in vitro propagated Micromeria pulegium (Rochel) Benth. (Lamiaceae)" in Planta, 244, no. 2 (2016):393-404,
https://doi.org/10.1007/s00425-016-2513-7 . .

TY  - JOUR
AU  - Živković, Jelena
AU  - Barreira, Joao C. M.
AU  - Stojković, Dejan
AU  - Cebović, Tatjana
AU  - Santos-Buelga, Celestino
AU  - Maksimović, Zoran
AU  - Ferreira, Isabel C. F. R.
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2211
AB  - The phenolic profile and antibacterial, antimutagenic and antitumour activities of the methanolic extracts of Veronica urticifolia Jacq. were evaluated. HPLC-DAD/ESI-MS analysis revealed the presence of phenolic acids, flavonoids and phenylethanoids, with acteoside as the main component (14.9 mg/g of extract). Antibacterial effect was determined using the microbroth dilution assay and Staphylococcus aureus was the most sensitive strain (MIC and MBC = 7.5 mg/mL). Antimutagenic activity was evaluated by Ames mutagenicity assay. At 1 mg/plate, the tested extract afforded high protection against the mutagenicity of nitroquinoline-N-oxide (4NQO) to Salmonella typhimurium strain TA100 (inhibition rate 48.3%). Antitumour activity was screened in Ehrlich ascites carcinoma (EAC) model. Pretreatment with 2 mg/kg body weight showed statistically significant decrease in tumour cell viability, while ascites volume and tumour cell count became slightly decreased, but not to a statistically significant extent. The results indicate that V. urticifolia deserves further research into its potential chemoprevention effects.
PB  - Elsevier Science BV, Amsterdam
T2  - Journal of Functional Foods
T1  - Phenolic profile, antibacterial, antimutagenic and antitumour evaluation of Veronica urticifolia Jacq.
VL  - 9
SP  - 192
EP  - 201
DO  - 10.1016/j.jff.2014.04.024
ER  - 

@article{
author = "Živković, Jelena and Barreira, Joao C. M. and Stojković, Dejan and Cebović, Tatjana and Santos-Buelga, Celestino and Maksimović, Zoran and Ferreira, Isabel C. F. R.",
year = "2014",
abstract = "The phenolic profile and antibacterial, antimutagenic and antitumour activities of the methanolic extracts of Veronica urticifolia Jacq. were evaluated. HPLC-DAD/ESI-MS analysis revealed the presence of phenolic acids, flavonoids and phenylethanoids, with acteoside as the main component (14.9 mg/g of extract). Antibacterial effect was determined using the microbroth dilution assay and Staphylococcus aureus was the most sensitive strain (MIC and MBC = 7.5 mg/mL). Antimutagenic activity was evaluated by Ames mutagenicity assay. At 1 mg/plate, the tested extract afforded high protection against the mutagenicity of nitroquinoline-N-oxide (4NQO) to Salmonella typhimurium strain TA100 (inhibition rate 48.3%). Antitumour activity was screened in Ehrlich ascites carcinoma (EAC) model. Pretreatment with 2 mg/kg body weight showed statistically significant decrease in tumour cell viability, while ascites volume and tumour cell count became slightly decreased, but not to a statistically significant extent. The results indicate that V. urticifolia deserves further research into its potential chemoprevention effects.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Journal of Functional Foods",
title = "Phenolic profile, antibacterial, antimutagenic and antitumour evaluation of Veronica urticifolia Jacq.",
volume = "9",
pages = "192-201",
doi = "10.1016/j.jff.2014.04.024"
}

Živković, J., Barreira, J. C. M., Stojković, D., Cebović, T., Santos-Buelga, C., Maksimović, Z.,& Ferreira, I. C. F. R.. (2014). Phenolic profile, antibacterial, antimutagenic and antitumour evaluation of Veronica urticifolia Jacq.. in Journal of Functional Foods
Elsevier Science BV, Amsterdam., 9, 192-201.
https://doi.org/10.1016/j.jff.2014.04.024

Živković J, Barreira JCM, Stojković D, Cebović T, Santos-Buelga C, Maksimović Z, Ferreira ICFR. Phenolic profile, antibacterial, antimutagenic and antitumour evaluation of Veronica urticifolia Jacq.. in Journal of Functional Foods. 2014;9:192-201.
doi:10.1016/j.jff.2014.04.024 .

Živković, Jelena, Barreira, Joao C. M., Stojković, Dejan, Cebović, Tatjana, Santos-Buelga, Celestino, Maksimović, Zoran, Ferreira, Isabel C. F. R., "Phenolic profile, antibacterial, antimutagenic and antitumour evaluation of Veronica urticifolia Jacq." in Journal of Functional Foods, 9 (2014):192-201,
https://doi.org/10.1016/j.jff.2014.04.024 . .