TY  - JOUR
AU  - Glišić, Milica
AU  - Bošković Cabrol, Marija
AU  - Čobanović, Nikola
AU  - Starčević, Marija
AU  - Samardžić, Stevan
AU  - Veličković, Ivona
AU  - Maksimović, Zoran
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5551
AB  - The present study aimed to evaluate the antioxidant capacity of ethanolic extracts from post-harvest sunflower and maize stalk residues, and their impact on the chemical composition, physicochemical parameters, lipid oxidative stability, microbiological properties, and sensory characteristics of pork liver pâtés over a 90-day storage period. Four formulations were prepared: a control group (CON), a batch with butylated hydroxytoluene as a synthetic antioxidant (BHT), 1% ethanolic extract from sunflower residues (SSRE), and 1% ethanolic extract from maize residues (MSRE). The MSRE had a higher total phenol content and showed better antioxidant activity relative to the SSRE (p < 0.01). The addition of SSRE decreased the lightness and increased the redness in the pork liver pâtés, with these pâtés showing the highest total color difference compared to the control (p < 0.01). The crop extracts increased the n-6 and total PUFA contents in pâtés and improved the PUFA/SFA ratio (p < 0.01). Formulations containing crop residue extracts showed higher TBARs and POV values than the control and BHT group (p < 0.01), indicating a pro-oxidant effect and accelerated lipid oxidation in pâtés during storage. As far as microbiological quality, the presence of crop residue extracts decreased the total viable count, lactic acid bacteria, and psychotropic aerobic bacteria (p < 0.01). The incorporation of crop extracts in the pork pâtés impaired their sensory quality, particularly color, odor, aroma, and flavor, and decreased their overall acceptability. These results indicated that, while the crop residue extracts were not as effective as synthetic antioxidants in preserving the lipid stability of pâtés, they demonstrated potential for enhancing the microbial quality of this type of meat product.
PB  - MDPI
T2  - Foods
T1  - The Effects of Sunflower and Maize Crop Residue Extracts as a New Ingredient on the Quality Properties of Pork Liver Pâtés
VL  - 13
IS  - 5
SP  - 788
DO  - 10.3390/foods13050788
ER  - 

@article{
author = "Glišić, Milica and Bošković Cabrol, Marija and Čobanović, Nikola and Starčević, Marija and Samardžić, Stevan and Veličković, Ivona and Maksimović, Zoran",
year = "2024",
abstract = "The present study aimed to evaluate the antioxidant capacity of ethanolic extracts from post-harvest sunflower and maize stalk residues, and their impact on the chemical composition, physicochemical parameters, lipid oxidative stability, microbiological properties, and sensory characteristics of pork liver pâtés over a 90-day storage period. Four formulations were prepared: a control group (CON), a batch with butylated hydroxytoluene as a synthetic antioxidant (BHT), 1% ethanolic extract from sunflower residues (SSRE), and 1% ethanolic extract from maize residues (MSRE). The MSRE had a higher total phenol content and showed better antioxidant activity relative to the SSRE (p < 0.01). The addition of SSRE decreased the lightness and increased the redness in the pork liver pâtés, with these pâtés showing the highest total color difference compared to the control (p < 0.01). The crop extracts increased the n-6 and total PUFA contents in pâtés and improved the PUFA/SFA ratio (p < 0.01). Formulations containing crop residue extracts showed higher TBARs and POV values than the control and BHT group (p < 0.01), indicating a pro-oxidant effect and accelerated lipid oxidation in pâtés during storage. As far as microbiological quality, the presence of crop residue extracts decreased the total viable count, lactic acid bacteria, and psychotropic aerobic bacteria (p < 0.01). The incorporation of crop extracts in the pork pâtés impaired their sensory quality, particularly color, odor, aroma, and flavor, and decreased their overall acceptability. These results indicated that, while the crop residue extracts were not as effective as synthetic antioxidants in preserving the lipid stability of pâtés, they demonstrated potential for enhancing the microbial quality of this type of meat product.",
publisher = "MDPI",
journal = "Foods",
title = "The Effects of Sunflower and Maize Crop Residue Extracts as a New Ingredient on the Quality Properties of Pork Liver Pâtés",
volume = "13",
number = "5",
pages = "788",
doi = "10.3390/foods13050788"
}

Glišić, M., Bošković Cabrol, M., Čobanović, N., Starčević, M., Samardžić, S., Veličković, I.,& Maksimović, Z.. (2024). The Effects of Sunflower and Maize Crop Residue Extracts as a New Ingredient on the Quality Properties of Pork Liver Pâtés. in Foods
MDPI., 13(5), 788.
https://doi.org/10.3390/foods13050788

Glišić M, Bošković Cabrol M, Čobanović N, Starčević M, Samardžić S, Veličković I, Maksimović Z. The Effects of Sunflower and Maize Crop Residue Extracts as a New Ingredient on the Quality Properties of Pork Liver Pâtés. in Foods. 2024;13(5):788.
doi:10.3390/foods13050788 .

Glišić, Milica, Bošković Cabrol, Marija, Čobanović, Nikola, Starčević, Marija, Samardžić, Stevan, Veličković, Ivona, Maksimović, Zoran, "The Effects of Sunflower and Maize Crop Residue Extracts as a New Ingredient on the Quality Properties of Pork Liver Pâtés" in Foods, 13, no. 5 (2024):788,
https://doi.org/10.3390/foods13050788 . .

TY  - CONF
AU  - Glišić, Milica
AU  - Bošković Cabrol, Marija
AU  - Čobanović, Nikola
AU  - Samardžić, Stevan
AU  - Baltić, Milan Ž.
AU  - Maksimović, Zoran
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5198
AB  - Modern agriculture produces a large amount of residue every year causing environmental pollution
due to limited and inadequate agricultural waste management. Previous research has shown that agro-industrial residues are important sources of various bioactive, mainly phenolic, compounds that could be applied in the meat industry as natural antimicrobials and antioxidants. This study aimed to assess the effect of the addition of sunflower and maize crop residue extracts on physicochemical (pH and colour) characteristics and microbiological quality of pork liver pâtés over three months of storage and compared to a synthetic antioxidant (butylated hydroxytoluene - BHT) and a control batch. The sunflower and maize crop ethanolic extracts improved the microbial quality of the pork liver pâtés indicating the potential of these extracts as natural preservatives in meat products. However, the addition of 1% of crop extracts resulted in a darker product with a higher b* value, especially after 3 months of storage.
C3  - 69th International Congress of Meat Science and Technology, August 20-25, 2023
Padova, Italy
T1  - Effect of additon of sunflower and maize crop residue extract on physicochemical and microbial properties of pork liver pâté
SP  - 579
EP  - 580
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5198
ER  - 

@conference{
author = "Glišić, Milica and Bošković Cabrol, Marija and Čobanović, Nikola and Samardžić, Stevan and Baltić, Milan Ž. and Maksimović, Zoran",
year = "2023",
abstract = "Modern agriculture produces a large amount of residue every year causing environmental pollution
due to limited and inadequate agricultural waste management. Previous research has shown that agro-industrial residues are important sources of various bioactive, mainly phenolic, compounds that could be applied in the meat industry as natural antimicrobials and antioxidants. This study aimed to assess the effect of the addition of sunflower and maize crop residue extracts on physicochemical (pH and colour) characteristics and microbiological quality of pork liver pâtés over three months of storage and compared to a synthetic antioxidant (butylated hydroxytoluene - BHT) and a control batch. The sunflower and maize crop ethanolic extracts improved the microbial quality of the pork liver pâtés indicating the potential of these extracts as natural preservatives in meat products. However, the addition of 1% of crop extracts resulted in a darker product with a higher b* value, especially after 3 months of storage.",
journal = "69th International Congress of Meat Science and Technology, August 20-25, 2023
Padova, Italy",
title = "Effect of additon of sunflower and maize crop residue extract on physicochemical and microbial properties of pork liver pâté",
pages = "579-580",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5198"
}

Glišić, M., Bošković Cabrol, M., Čobanović, N., Samardžić, S., Baltić, M. Ž.,& Maksimović, Z.. (2023). Effect of additon of sunflower and maize crop residue extract on physicochemical and microbial properties of pork liver pâté. in 69th International Congress of Meat Science and Technology, August 20-25, 2023
Padova, Italy, 579-580.
https://hdl.handle.net/21.15107/rcub_farfar_5198

Glišić M, Bošković Cabrol M, Čobanović N, Samardžić S, Baltić MŽ, Maksimović Z. Effect of additon of sunflower and maize crop residue extract on physicochemical and microbial properties of pork liver pâté. in 69th International Congress of Meat Science and Technology, August 20-25, 2023
Padova, Italy. 2023;:579-580.
https://hdl.handle.net/21.15107/rcub_farfar_5198 .

Glišić, Milica, Bošković Cabrol, Marija, Čobanović, Nikola, Samardžić, Stevan, Baltić, Milan Ž., Maksimović, Zoran, "Effect of additon of sunflower and maize crop residue extract on physicochemical and microbial properties of pork liver pâté" in 69th International Congress of Meat Science and Technology, August 20-25, 2023
Padova, Italy (2023):579-580,
https://hdl.handle.net/21.15107/rcub_farfar_5198 .

TY  - JOUR
AU  - Glišić, Milica
AU  - Bošković Cabrol, Marija
AU  - Čobanović, Nikola
AU  - Baltić, Milan Ž.
AU  - Vranešević, Jelena
AU  - Samardžić, Stevan
AU  - Maksimović, Zoran
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4954
AB  - Large quantities of agricultural residues are generated every year. Most of the crop-based residues are underutilized, mainly left to decay on the land or to be burnt, which can lead to an increase in a load of environmental pollution. Considering this, different strategies have been developed to use these renewable resources as raw materials for the production of bioactive compounds, their isolation and characterization, and potential application in a wide range of fields, particularly in the food industry as natural preservatives. In this study, the antibacterial efficacy of wheat, sunflower, and maize crop residue ethanolic extracts against six bacterial strains (Salmonella Typhimurium, Salmonella Enteritidis, Staphylococcus aureus, Escherichia coli, Listeria monocytogenes and Yersinia enterocolitica) was evaluated by the broth microdilution method. Used extracts inhibited the growth of selected microorganisms with a minimal inhibitory concentration (MIC) of 320 μg/mL for most of the tested bacteria. L. monocytogenes showed a MIC value of 640 μg/mL for wheat ethanolic extract, and the MIC value of sunflower ethanolic extract for S. Typhimurium was 160 μg/mL. There were no minimum bactericidal concentration (MBC) values for any of the bacteria within the extract’s concentration ranges tested (≤ 2560 μg/mL). The results of the present study indicate that crop residue ethanolic extracts could exhibit bacteriostatic effect and therefore have the potential as natural additives in food preservation.
AB  - Velika količina poljoprivrednih ostataka se proizvodi svake godine. Većina žetvenih ostataka je nedovoljno iskorišćena, uglavnom se ostavlja da propadne na njivama ili se spaljuje, što dovodi do povećanog zagađenja životne sredine. Imajući ovo u vidu, razvijene su različite strategije za iskorišćavanje navedenih obnovljivih resursa kao sirovina u proizvodnji bioaktivnih jedinjenja, njihovu izolaciju i karakterizaciju i potencijalnu primenu u različitim oblastima, naročito u industriji hrane kao prirodni konzervansi. U ovoj studiji ispitivana je antibakterijska efikasnost etanolnih ekstrakata žetvenih ostataka pšenice, suncokreta i kukuruza na šest bakterijskih sojeva (Salmonella Typhimurium, Salmonella Enteritidis, Staphylococcus aureus, Escherichia coli, Listeria monocytogenes i Yersinia enterocolitica) primenom mikrodilucione metode u bujonu. Ekstrakti žetvenih ostataka su inhibirali rast odabranih mikroorganizama u minimalnoj inhibitornoj koncentraciji (MIC) od 320 μg/mL za većinu ispitivanih bakterija. Za L. monocytogenes MIC vrednost etanolnog ekstrakta pšenice bila je 640 μg/ mL, a 160 μg/mL je bila MIC vrednost etanolnog ekstrakta suncokreta za S. Typhimurium. Minimalna baktericidna koncentracija (MBC) prema bakterijama nije postignuta u ispitivanim koncentracijama etanolnih ekstrakata (≤ 2560 μg/mL). Rezultati ukazuju da etanolni ekstrakti žetvenih rezidua bi mogli da ostvare antibakterijski efekat i stoga imaju potencijal kao prirodni aditivi u konzervisanju hrane.
PB  - Scientific Veterinary Institute "Novi Sad"
T2  - Archives of Veterinary Medicine
T1  - Antimicrobial activity of ethanolic extracts from wheat, sunflower and maize crop residues
T1  - Antibakterijska aktivnost etanolnih ekstrakata žetvenih ostataka pšenice, suncokreta i kukuruza
VL  - 16
IS  - 1
SP  - 53
EP  - 67
DO  - 10.46784/e-avm.v16i1.315
ER  - 

@article{
author = "Glišić, Milica and Bošković Cabrol, Marija and Čobanović, Nikola and Baltić, Milan Ž. and Vranešević, Jelena and Samardžić, Stevan and Maksimović, Zoran",
year = "2023",
abstract = "Large quantities of agricultural residues are generated every year. Most of the crop-based residues are underutilized, mainly left to decay on the land or to be burnt, which can lead to an increase in a load of environmental pollution. Considering this, different strategies have been developed to use these renewable resources as raw materials for the production of bioactive compounds, their isolation and characterization, and potential application in a wide range of fields, particularly in the food industry as natural preservatives. In this study, the antibacterial efficacy of wheat, sunflower, and maize crop residue ethanolic extracts against six bacterial strains (Salmonella Typhimurium, Salmonella Enteritidis, Staphylococcus aureus, Escherichia coli, Listeria monocytogenes and Yersinia enterocolitica) was evaluated by the broth microdilution method. Used extracts inhibited the growth of selected microorganisms with a minimal inhibitory concentration (MIC) of 320 μg/mL for most of the tested bacteria. L. monocytogenes showed a MIC value of 640 μg/mL for wheat ethanolic extract, and the MIC value of sunflower ethanolic extract for S. Typhimurium was 160 μg/mL. There were no minimum bactericidal concentration (MBC) values for any of the bacteria within the extract’s concentration ranges tested (≤ 2560 μg/mL). The results of the present study indicate that crop residue ethanolic extracts could exhibit bacteriostatic effect and therefore have the potential as natural additives in food preservation., Velika količina poljoprivrednih ostataka se proizvodi svake godine. Većina žetvenih ostataka je nedovoljno iskorišćena, uglavnom se ostavlja da propadne na njivama ili se spaljuje, što dovodi do povećanog zagađenja životne sredine. Imajući ovo u vidu, razvijene su različite strategije za iskorišćavanje navedenih obnovljivih resursa kao sirovina u proizvodnji bioaktivnih jedinjenja, njihovu izolaciju i karakterizaciju i potencijalnu primenu u različitim oblastima, naročito u industriji hrane kao prirodni konzervansi. U ovoj studiji ispitivana je antibakterijska efikasnost etanolnih ekstrakata žetvenih ostataka pšenice, suncokreta i kukuruza na šest bakterijskih sojeva (Salmonella Typhimurium, Salmonella Enteritidis, Staphylococcus aureus, Escherichia coli, Listeria monocytogenes i Yersinia enterocolitica) primenom mikrodilucione metode u bujonu. Ekstrakti žetvenih ostataka su inhibirali rast odabranih mikroorganizama u minimalnoj inhibitornoj koncentraciji (MIC) od 320 μg/mL za većinu ispitivanih bakterija. Za L. monocytogenes MIC vrednost etanolnog ekstrakta pšenice bila je 640 μg/ mL, a 160 μg/mL je bila MIC vrednost etanolnog ekstrakta suncokreta za S. Typhimurium. Minimalna baktericidna koncentracija (MBC) prema bakterijama nije postignuta u ispitivanim koncentracijama etanolnih ekstrakata (≤ 2560 μg/mL). Rezultati ukazuju da etanolni ekstrakti žetvenih rezidua bi mogli da ostvare antibakterijski efekat i stoga imaju potencijal kao prirodni aditivi u konzervisanju hrane.",
publisher = "Scientific Veterinary Institute "Novi Sad"",
journal = "Archives of Veterinary Medicine",
title = "Antimicrobial activity of ethanolic extracts from wheat, sunflower and maize crop residues, Antibakterijska aktivnost etanolnih ekstrakata žetvenih ostataka pšenice, suncokreta i kukuruza",
volume = "16",
number = "1",
pages = "53-67",
doi = "10.46784/e-avm.v16i1.315"
}

Glišić, M., Bošković Cabrol, M., Čobanović, N., Baltić, M. Ž., Vranešević, J., Samardžić, S.,& Maksimović, Z.. (2023). Antimicrobial activity of ethanolic extracts from wheat, sunflower and maize crop residues. in Archives of Veterinary Medicine
Scientific Veterinary Institute "Novi Sad"., 16(1), 53-67.
https://doi.org/10.46784/e-avm.v16i1.315

Glišić M, Bošković Cabrol M, Čobanović N, Baltić MŽ, Vranešević J, Samardžić S, Maksimović Z. Antimicrobial activity of ethanolic extracts from wheat, sunflower and maize crop residues. in Archives of Veterinary Medicine. 2023;16(1):53-67.
doi:10.46784/e-avm.v16i1.315 .

Glišić, Milica, Bošković Cabrol, Marija, Čobanović, Nikola, Baltić, Milan Ž., Vranešević, Jelena, Samardžić, Stevan, Maksimović, Zoran, "Antimicrobial activity of ethanolic extracts from wheat, sunflower and maize crop residues" in Archives of Veterinary Medicine, 16, no. 1 (2023):53-67,
https://doi.org/10.46784/e-avm.v16i1.315 . .

TY  - CONF
AU  - Glišić, Milica
AU  - Bošković-Cabrol, Marija
AU  - Čobanović, Nikola
AU  - Baltić, Milan Ž.
AU  - Drašković, Vladimir
AU  - Samardžić, Stevan
AU  - Maksimović, Zoran
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5199
AB  - Globalization and population growth have led to the development of a modern agricultural system that currently produces millions of tons of waste. This waste is disposed of by burning, dumping or accumulating in landfills, resulting in environmental, health, and economic issues.The agro‑industrial residues are abundant with phenolic bioactive compounds, such as phenolic acids, flavonoids, tannins, and carotenoids, which, among others, exhibit antioxidant and antimicrobial capacities and have good potential as food flavorings and colorants. The most common method for isolating these compounds is solvent extraction. However, there is a trend towards eco‑innovative extraction methods that offer better possibilities for implementation on an industrial scale. The oxidation of lipids and proteins is one of the main causes of quality deterioration in meat and meat products during processing and storage. Therefore, the application of natural antioxidants extracted from these new, unconventional raw materials could be a sustainable alternative to synthetic antioxidants. This review summarizes the data on natural antioxidants derived from agro‑industrial by‑products and their incorporation in various meat product formulations. It also addresses limiting factors related to safety and changes in sensory properties.
PB  - Institute of Meat Hygiene and Technology
C3  - Meat Technology - Special Issue
T1  - Agricultural Waste: A Source of Bioactive Compounds for Potential Application in Meat Products
VL  - 64
IS  - 2
SP  - 116
EP  - 121
DO  - 10.18485/meattech.2023.64.2.20
ER  - 

@conference{
author = "Glišić, Milica and Bošković-Cabrol, Marija and Čobanović, Nikola and Baltić, Milan Ž. and Drašković, Vladimir and Samardžić, Stevan and Maksimović, Zoran",
year = "2023",
abstract = "Globalization and population growth have led to the development of a modern agricultural system that currently produces millions of tons of waste. This waste is disposed of by burning, dumping or accumulating in landfills, resulting in environmental, health, and economic issues.The agro‑industrial residues are abundant with phenolic bioactive compounds, such as phenolic acids, flavonoids, tannins, and carotenoids, which, among others, exhibit antioxidant and antimicrobial capacities and have good potential as food flavorings and colorants. The most common method for isolating these compounds is solvent extraction. However, there is a trend towards eco‑innovative extraction methods that offer better possibilities for implementation on an industrial scale. The oxidation of lipids and proteins is one of the main causes of quality deterioration in meat and meat products during processing and storage. Therefore, the application of natural antioxidants extracted from these new, unconventional raw materials could be a sustainable alternative to synthetic antioxidants. This review summarizes the data on natural antioxidants derived from agro‑industrial by‑products and their incorporation in various meat product formulations. It also addresses limiting factors related to safety and changes in sensory properties.",
publisher = "Institute of Meat Hygiene and Technology",
journal = "Meat Technology - Special Issue",
title = "Agricultural Waste: A Source of Bioactive Compounds for Potential Application in Meat Products",
volume = "64",
number = "2",
pages = "116-121",
doi = "10.18485/meattech.2023.64.2.20"
}

Glišić, M., Bošković-Cabrol, M., Čobanović, N., Baltić, M. Ž., Drašković, V., Samardžić, S.,& Maksimović, Z.. (2023). Agricultural Waste: A Source of Bioactive Compounds for Potential Application in Meat Products. in Meat Technology - Special Issue
Institute of Meat Hygiene and Technology., 64(2), 116-121.
https://doi.org/10.18485/meattech.2023.64.2.20

Glišić M, Bošković-Cabrol M, Čobanović N, Baltić MŽ, Drašković V, Samardžić S, Maksimović Z. Agricultural Waste: A Source of Bioactive Compounds for Potential Application in Meat Products. in Meat Technology - Special Issue. 2023;64(2):116-121.
doi:10.18485/meattech.2023.64.2.20 .

Glišić, Milica, Bošković-Cabrol, Marija, Čobanović, Nikola, Baltić, Milan Ž., Drašković, Vladimir, Samardžić, Stevan, Maksimović, Zoran, "Agricultural Waste: A Source of Bioactive Compounds for Potential Application in Meat Products" in Meat Technology - Special Issue, 64, no. 2 (2023):116-121,
https://doi.org/10.18485/meattech.2023.64.2.20 . .

TY  - CONF
AU  - Glišić, Milica
AU  - Bošković Cabrol, Marija
AU  - Baltić, Milan
AU  - Maksimović, Zoran
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4006
AB  - Due to the growing health problems associated with the increased intake of saturated and trans fats, and the unbalanced n-6/n-3 ratio in the diet, in recent years numerous studies have focused on finding adequate substitutes for fat in meat products, while the meat industry made additional efforts to implement the obtained formulations (oleogels) in the standard production processes. Insoluble cellulose fibre in the form of microcrystalline cellulose has proven to be a promising ingredient in reduced fat, fibre-enriched functional food development, since it has been safely used as a food additive for many years with a known beneficial effect on human health. This review will discuss the recent advances of MCC application associated with alternative cellulose sources and processing technology, functional physico-chemical properties and potential as organogelator in fat mimetics. Finally, recently published data concerning its practical application in meat products as fat or starch substitutes will be presented.
PB  - IOP Publishing Ltd
C3  - IOP Conference Series: Earth and Environmental Science
T1  - Cellulose hydrocolloids in meat products: Current status and challenges in developing functional food
VL  - 854
IS  - 1
DO  - 10.1088/1755-1315/854/1/012030
ER  - 

@conference{
author = "Glišić, Milica and Bošković Cabrol, Marija and Baltić, Milan and Maksimović, Zoran",
year = "2021",
abstract = "Due to the growing health problems associated with the increased intake of saturated and trans fats, and the unbalanced n-6/n-3 ratio in the diet, in recent years numerous studies have focused on finding adequate substitutes for fat in meat products, while the meat industry made additional efforts to implement the obtained formulations (oleogels) in the standard production processes. Insoluble cellulose fibre in the form of microcrystalline cellulose has proven to be a promising ingredient in reduced fat, fibre-enriched functional food development, since it has been safely used as a food additive for many years with a known beneficial effect on human health. This review will discuss the recent advances of MCC application associated with alternative cellulose sources and processing technology, functional physico-chemical properties and potential as organogelator in fat mimetics. Finally, recently published data concerning its practical application in meat products as fat or starch substitutes will be presented.",
publisher = "IOP Publishing Ltd",
journal = "IOP Conference Series: Earth and Environmental Science",
title = "Cellulose hydrocolloids in meat products: Current status and challenges in developing functional food",
volume = "854",
number = "1",
doi = "10.1088/1755-1315/854/1/012030"
}

Glišić, M., Bošković Cabrol, M., Baltić, M.,& Maksimović, Z.. (2021). Cellulose hydrocolloids in meat products: Current status and challenges in developing functional food. in IOP Conference Series: Earth and Environmental Science
IOP Publishing Ltd., 854(1).
https://doi.org/10.1088/1755-1315/854/1/012030

Glišić M, Bošković Cabrol M, Baltić M, Maksimović Z. Cellulose hydrocolloids in meat products: Current status and challenges in developing functional food. in IOP Conference Series: Earth and Environmental Science. 2021;854(1).
doi:10.1088/1755-1315/854/1/012030 .

Glišić, Milica, Bošković Cabrol, Marija, Baltić, Milan, Maksimović, Zoran, "Cellulose hydrocolloids in meat products: Current status and challenges in developing functional food" in IOP Conference Series: Earth and Environmental Science, 854, no. 1 (2021),
https://doi.org/10.1088/1755-1315/854/1/012030 . .

TY  - JOUR
AU  - Stevanović, Strahinja
AU  - Marjanović, Đorđe
AU  - Trailović, Saša
AU  - Zdravković, Nemanja
AU  - Perdih, Andrej
AU  - Nikolić, Katarina
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3766
AB  - Parasitic infections are a widespread health problem and research of novel anthelmintic compounds is of the utmost importance. In this study we performed a virtual screening campaign by coupling ligand-based pharmacophore, homology modeling and molecular docking. The virtual screening campaign was conducted using a joined pool of the Drugbank database and a library of purchasable compounds in order to identify drug like compounds with similar pharmacological activity. Our aim was to identify compounds with a potential antihelmintic modulatory effect on nicotinic acetylcholine receptors (nAChR). We derived a 3D pharmacophore model based on the chemical features of known Ascaris suum nAChR modulators. To evaluate the in silico predictions, we tested selected hit compounds in contraction assays using somatic muscle flaps of the Ascaris suum neuromuscular tissue. We tested the modulatory effects of GSK575594A, diazepam and flumazenil hit compounds on nematode contractions induced by acetyl choline (ACh). The compound GSK575594A (3 μM) increased the Emax by 21 % with the EC50 dose ratio of 0.96. Diazepam (100 μM) decreased the Emax by 15 % (1.11 g to 0.95 g) with the EC50 ratio of 1.42 (shifted to the left from 11.25 to 7.93). Flumazenil decreased the EC50 value (from 11.22 μM to 4.88 μM) value showing dose ratio of 2.30, and increased the Emax by 4 % (from 1.54 g to 1.59 g). The observed biological activity was rationalized by molecular docking calculations. Docking scores were calculated against several binding sites within the Ascaris suum homology model. We constructed the homology model using the ACR-16 subunit sequence. The compound GSK575594A showed strong affinity for the intersubunit allosteric binding site within the nAChR transmembrane domain. The binding modes of diazepam and flumazenil suggest that these compounds have a comparable affinity for orthosteric and allosteric nAChR binding sites. The selected hit compounds displayed potential for further optimization as lead compounds. Therefore, such compounds may be useful in neutralizing the growing resistance of parasites to drugs, either alone or in combination with existing conventional anthelmintics.
PB  - Elsevier B.V.
T2  - Molecular and Biochemical Parasitology
T1  - Potential modulating effect of the Ascaris suum nicotinic acetylcholine receptor (nAChR) by compounds GSK575594A, diazepam and flumazenil discovered by structure-based virtual screening approach
VL  - 242
DO  - 10.1016/j.molbiopara.2021.111350
ER  - 

@article{
author = "Stevanović, Strahinja and Marjanović, Đorđe and Trailović, Saša and Zdravković, Nemanja and Perdih, Andrej and Nikolić, Katarina",
year = "2021",
abstract = "Parasitic infections are a widespread health problem and research of novel anthelmintic compounds is of the utmost importance. In this study we performed a virtual screening campaign by coupling ligand-based pharmacophore, homology modeling and molecular docking. The virtual screening campaign was conducted using a joined pool of the Drugbank database and a library of purchasable compounds in order to identify drug like compounds with similar pharmacological activity. Our aim was to identify compounds with a potential antihelmintic modulatory effect on nicotinic acetylcholine receptors (nAChR). We derived a 3D pharmacophore model based on the chemical features of known Ascaris suum nAChR modulators. To evaluate the in silico predictions, we tested selected hit compounds in contraction assays using somatic muscle flaps of the Ascaris suum neuromuscular tissue. We tested the modulatory effects of GSK575594A, diazepam and flumazenil hit compounds on nematode contractions induced by acetyl choline (ACh). The compound GSK575594A (3 μM) increased the Emax by 21 % with the EC50 dose ratio of 0.96. Diazepam (100 μM) decreased the Emax by 15 % (1.11 g to 0.95 g) with the EC50 ratio of 1.42 (shifted to the left from 11.25 to 7.93). Flumazenil decreased the EC50 value (from 11.22 μM to 4.88 μM) value showing dose ratio of 2.30, and increased the Emax by 4 % (from 1.54 g to 1.59 g). The observed biological activity was rationalized by molecular docking calculations. Docking scores were calculated against several binding sites within the Ascaris suum homology model. We constructed the homology model using the ACR-16 subunit sequence. The compound GSK575594A showed strong affinity for the intersubunit allosteric binding site within the nAChR transmembrane domain. The binding modes of diazepam and flumazenil suggest that these compounds have a comparable affinity for orthosteric and allosteric nAChR binding sites. The selected hit compounds displayed potential for further optimization as lead compounds. Therefore, such compounds may be useful in neutralizing the growing resistance of parasites to drugs, either alone or in combination with existing conventional anthelmintics.",
publisher = "Elsevier B.V.",
journal = "Molecular and Biochemical Parasitology",
title = "Potential modulating effect of the Ascaris suum nicotinic acetylcholine receptor (nAChR) by compounds GSK575594A, diazepam and flumazenil discovered by structure-based virtual screening approach",
volume = "242",
doi = "10.1016/j.molbiopara.2021.111350"
}

Stevanović, S., Marjanović, Đ., Trailović, S., Zdravković, N., Perdih, A.,& Nikolić, K.. (2021). Potential modulating effect of the Ascaris suum nicotinic acetylcholine receptor (nAChR) by compounds GSK575594A, diazepam and flumazenil discovered by structure-based virtual screening approach. in Molecular and Biochemical Parasitology
Elsevier B.V.., 242.
https://doi.org/10.1016/j.molbiopara.2021.111350

Stevanović S, Marjanović Đ, Trailović S, Zdravković N, Perdih A, Nikolić K. Potential modulating effect of the Ascaris suum nicotinic acetylcholine receptor (nAChR) by compounds GSK575594A, diazepam and flumazenil discovered by structure-based virtual screening approach. in Molecular and Biochemical Parasitology. 2021;242.
doi:10.1016/j.molbiopara.2021.111350 .

Stevanović, Strahinja, Marjanović, Đorđe, Trailović, Saša, Zdravković, Nemanja, Perdih, Andrej, Nikolić, Katarina, "Potential modulating effect of the Ascaris suum nicotinic acetylcholine receptor (nAChR) by compounds GSK575594A, diazepam and flumazenil discovered by structure-based virtual screening approach" in Molecular and Biochemical Parasitology, 242 (2021),
https://doi.org/10.1016/j.molbiopara.2021.111350 . .

TY  - JOUR
AU  - Ćupić Miladinović, Dejana
AU  - Prevendar Crnić, Andreja
AU  - Peković, Sanja
AU  - Dacić, Sanja
AU  - Ivanović, Saša
AU  - Santibanez, Juan Francisco
AU  - Ćupić, Vitomir
AU  - Borozan, Nevena
AU  - Antonijević-Miljaković, Evica
AU  - Borozan, Sunčica
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3810
AB  - Chlorpyrifos is a extensively used organophosphate pesticide (OP). In this study, we closely looked into neurotoxicity of CPF and effect of vitamin B1, by checking the levels of cholinesterases, determining the activity of parameters of oxidative stress, inflammation and also level of apoptotic regulator. The study was performed on a total of 80 male Japanese quails (Coturnix japonica), (two control and 6 experimental groups, n = 10). Three group of quails were given by gavage chlorpyrifos (CPF) for 7 consecutive days at doses of 1.50 mg/kg b.w., 3.00 mg/kg b.w., and 6.00 mg/kg b.w. Another three groups were treated with 10 mg/kg b.w. of vitamin B1 i.m. 30 min after CPF application (in above mentioned doses). Our study have proved that all doses of CPF significantly inhibited cholinesterases in brain, while vitamin B1 reactivated them. CPF has led to an increase in the concentration of malondialdehyde (MDA), and activity of catalase (CAT), superoxide dismutase (SOD), glutathione-S-transferase (GST), while tiamin changed the activity of antioxidant enzymes: CAT, SOD, GST. CPF stimulated apoptosis by decreasing B-cell lymphoma (Bcl-2) in brain, while application of vitamin B1 caused an increase of this parameter. CPF amplified inflammatory effect by elevating levels of inducible nitric oxide synthase (iNOS), and cyclooxygenase (COX-2). Thiamine proved its anti-inflammatory property by decreasing the expression of iNOS and interleukin-1(IL-1) and interleukin-6(IL-6). This study is highly pertinent because there is little defense currently available to humans and animals to prevent toxic effects of pesticides.
PB  - Elsevier Ireland Ltd
T2  - Chemico-Biological Interactions
T1  - Recovery of brain cholinesterases and effect on parameters of oxidative stres and apoptosis in quails (Coturnix japonica) after chlorpyrifos and vitamin B1 administration
VL  - 333
DO  - 10.1016/j.cbi.2020.109312
ER  - 

@article{
author = "Ćupić Miladinović, Dejana and Prevendar Crnić, Andreja and Peković, Sanja and Dacić, Sanja and Ivanović, Saša and Santibanez, Juan Francisco and Ćupić, Vitomir and Borozan, Nevena and Antonijević-Miljaković, Evica and Borozan, Sunčica",
year = "2021",
abstract = "Chlorpyrifos is a extensively used organophosphate pesticide (OP). In this study, we closely looked into neurotoxicity of CPF and effect of vitamin B1, by checking the levels of cholinesterases, determining the activity of parameters of oxidative stress, inflammation and also level of apoptotic regulator. The study was performed on a total of 80 male Japanese quails (Coturnix japonica), (two control and 6 experimental groups, n = 10). Three group of quails were given by gavage chlorpyrifos (CPF) for 7 consecutive days at doses of 1.50 mg/kg b.w., 3.00 mg/kg b.w., and 6.00 mg/kg b.w. Another three groups were treated with 10 mg/kg b.w. of vitamin B1 i.m. 30 min after CPF application (in above mentioned doses). Our study have proved that all doses of CPF significantly inhibited cholinesterases in brain, while vitamin B1 reactivated them. CPF has led to an increase in the concentration of malondialdehyde (MDA), and activity of catalase (CAT), superoxide dismutase (SOD), glutathione-S-transferase (GST), while tiamin changed the activity of antioxidant enzymes: CAT, SOD, GST. CPF stimulated apoptosis by decreasing B-cell lymphoma (Bcl-2) in brain, while application of vitamin B1 caused an increase of this parameter. CPF amplified inflammatory effect by elevating levels of inducible nitric oxide synthase (iNOS), and cyclooxygenase (COX-2). Thiamine proved its anti-inflammatory property by decreasing the expression of iNOS and interleukin-1(IL-1) and interleukin-6(IL-6). This study is highly pertinent because there is little defense currently available to humans and animals to prevent toxic effects of pesticides.",
publisher = "Elsevier Ireland Ltd",
journal = "Chemico-Biological Interactions",
title = "Recovery of brain cholinesterases and effect on parameters of oxidative stres and apoptosis in quails (Coturnix japonica) after chlorpyrifos and vitamin B1 administration",
volume = "333",
doi = "10.1016/j.cbi.2020.109312"
}

Ćupić Miladinović, D., Prevendar Crnić, A., Peković, S., Dacić, S., Ivanović, S., Santibanez, J. F., Ćupić, V., Borozan, N., Antonijević-Miljaković, E.,& Borozan, S.. (2021). Recovery of brain cholinesterases and effect on parameters of oxidative stres and apoptosis in quails (Coturnix japonica) after chlorpyrifos and vitamin B1 administration. in Chemico-Biological Interactions
Elsevier Ireland Ltd., 333.
https://doi.org/10.1016/j.cbi.2020.109312

Ćupić Miladinović D, Prevendar Crnić A, Peković S, Dacić S, Ivanović S, Santibanez JF, Ćupić V, Borozan N, Antonijević-Miljaković E, Borozan S. Recovery of brain cholinesterases and effect on parameters of oxidative stres and apoptosis in quails (Coturnix japonica) after chlorpyrifos and vitamin B1 administration. in Chemico-Biological Interactions. 2021;333.
doi:10.1016/j.cbi.2020.109312 .

Ćupić Miladinović, Dejana, Prevendar Crnić, Andreja, Peković, Sanja, Dacić, Sanja, Ivanović, Saša, Santibanez, Juan Francisco, Ćupić, Vitomir, Borozan, Nevena, Antonijević-Miljaković, Evica, Borozan, Sunčica, "Recovery of brain cholinesterases and effect on parameters of oxidative stres and apoptosis in quails (Coturnix japonica) after chlorpyrifos and vitamin B1 administration" in Chemico-Biological Interactions, 333 (2021),
https://doi.org/10.1016/j.cbi.2020.109312 . .