TY  - JOUR
AU  - Pavun, Leposava
AU  - Đikanović, Daniela
AU  - Đurđević, Predrag
AU  - Jelikić-Stankov, Milena
AU  - Malešev, Dušan
AU  - Ćirić, Andrija
PY  - 2009
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1205
AB  - Morin is a flavonol antioxidant. In ethanol-water mixtures (70 wt% of ethanol) it reacts with Al(3+) to give Al(Morin)(2) in the pH range 3-6. The conditional stability constant of this complex at 298 K was found to be log beta(2) = 16.96 +/- 0.02 at pH 4.40. The complex shows strong fluorescence emission at 500 nm upon excitation at 410 nm. The fluorescence intensity is pH dependent with maximum emission at pH 4.40. Since the complexation reaction enhances the fluorescence of morin, this property was used for the determination of morin in human serum. A linear dependence of the intensity of fluorescence of the complex on the concentration of morin was obtained in morin concentration range from 1.5-30.5 ng mL(-1), relative standard error of measurements was 1.4%. The LOD was 0.02 ng mL(-1) while LOQ was 1.0 ng mL(-1). Serum concentration of morin was also determined using HPLC as a reference method. A C-18 Hypersil Gold AQ column was used with acetonitrile-0.1% v/v phosphoric acid (30:70% v/v) as the mobile phase at 1.0 mL min(-1) flow rate and UV detection at 250 nm. Acceptable relative standard errors (less than 5%) between determinations obtained by the two methods indicate that the fluorescence method is reliable.
PB  - Slovensko Kemijsko Drustvo, Ljubljana
T2  - Acta Chimica Slovenica
T1  - Spectrofluorimetric and HPLC Determination of Morin in Human Serum
VL  - 56
IS  - 4
SP  - 967
EP  - 972
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1205
ER  - 

@article{
author = "Pavun, Leposava and Đikanović, Daniela and Đurđević, Predrag and Jelikić-Stankov, Milena and Malešev, Dušan and Ćirić, Andrija",
year = "2009",
abstract = "Morin is a flavonol antioxidant. In ethanol-water mixtures (70 wt% of ethanol) it reacts with Al(3+) to give Al(Morin)(2) in the pH range 3-6. The conditional stability constant of this complex at 298 K was found to be log beta(2) = 16.96 +/- 0.02 at pH 4.40. The complex shows strong fluorescence emission at 500 nm upon excitation at 410 nm. The fluorescence intensity is pH dependent with maximum emission at pH 4.40. Since the complexation reaction enhances the fluorescence of morin, this property was used for the determination of morin in human serum. A linear dependence of the intensity of fluorescence of the complex on the concentration of morin was obtained in morin concentration range from 1.5-30.5 ng mL(-1), relative standard error of measurements was 1.4%. The LOD was 0.02 ng mL(-1) while LOQ was 1.0 ng mL(-1). Serum concentration of morin was also determined using HPLC as a reference method. A C-18 Hypersil Gold AQ column was used with acetonitrile-0.1% v/v phosphoric acid (30:70% v/v) as the mobile phase at 1.0 mL min(-1) flow rate and UV detection at 250 nm. Acceptable relative standard errors (less than 5%) between determinations obtained by the two methods indicate that the fluorescence method is reliable.",
publisher = "Slovensko Kemijsko Drustvo, Ljubljana",
journal = "Acta Chimica Slovenica",
title = "Spectrofluorimetric and HPLC Determination of Morin in Human Serum",
volume = "56",
number = "4",
pages = "967-972",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1205"
}

Pavun, L., Đikanović, D., Đurđević, P., Jelikić-Stankov, M., Malešev, D.,& Ćirić, A.. (2009). Spectrofluorimetric and HPLC Determination of Morin in Human Serum. in Acta Chimica Slovenica
Slovensko Kemijsko Drustvo, Ljubljana., 56(4), 967-972.
https://hdl.handle.net/21.15107/rcub_farfar_1205

Pavun L, Đikanović D, Đurđević P, Jelikić-Stankov M, Malešev D, Ćirić A. Spectrofluorimetric and HPLC Determination of Morin in Human Serum. in Acta Chimica Slovenica. 2009;56(4):967-972.
https://hdl.handle.net/21.15107/rcub_farfar_1205 .

Pavun, Leposava, Đikanović, Daniela, Đurđević, Predrag, Jelikić-Stankov, Milena, Malešev, Dušan, Ćirić, Andrija, "Spectrofluorimetric and HPLC Determination of Morin in Human Serum" in Acta Chimica Slovenica, 56, no. 4 (2009):967-972,
https://hdl.handle.net/21.15107/rcub_farfar_1205 .

TY  - JOUR
AU  - Malešev, Dušan
AU  - Kuntić, Vesna
PY  - 2007
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/998
AB  - Flavonoids constitute a large group of polyphenolic phytochemicals with antioxidant properties which are overwhelmingly exerted through direct free radical scavenging. Flavonoids also exhibit antioxidant properties through chelating with transition metals, primarily Fe(II), Fe(III) and Cu(II), which participate in reactions generating free radicals. Metal-flavonoid chelates are considerably more potent free radical scavengers than the parent flavonoids and play a prominent role in protecting from oxidative stress. To unravel the origin of their potent biological action extensive physico-chemical studies were undertaken to reveal the chemical structure, chelation sites, assess the impact of the metal/ligand ratio on the structure of the complexes and the capacity of flavonoids to bind metal ions. In spite of such extensive efforts, data on the composition, structure and complex-formation properties are incomplete and sometimes even contradictory. The aim of this paper is to give a personal account on the development of the field through a retrospective evaluation of our own research which covers approximately 40 complexes of flavonoids from different flavonoids subclasses (rutin, quercetin, 3-hydroxyflavone, morin and hesperidin) with several metal ions or groups and suggest directions for future research. Special emphasis will be given to the site of the central ion, the composition of the complexes, the role of pH in complex formation, the stability of metal-flavonoid complexes and their potential application for analytical purposes. .
AB  - Flavonoidi predstavljaju veliku grupu polifenolnih jedinjenja biljnog porekla, koji imaju antioksidantna svojstva zbog direktnog "hvatanja" slobodnih radikala. Antioksidantno dejstvo flavonoidi takođe ostvaruju stvaranjem helata sa jonima prelaznih metala, prvenstveno sa jonima Fe(II), Fe(III) i Cu(II), koji sami učestvuju u reakciji stvaranja slobodnih radikala. Helati metal-flavonoid su mnogo efikasniji "hvatači" slobodnih radikala od matičnih flavonoida i mogu zaštititi potencijalne mete u organizmu od oksidativnog stresa. Da bi se razjasnio mehanizam biološkog delovanja kompleksa metal-flavonoid, ispitivane su fizičko-hemijske karakteristike kompleksa: mesto vezivanja jona metala, zavisnost strukture kompleksa od odnosa metal/ligand, afinitet flavonoida za vezivanje metalnog jona, itd. Međutim, podaci o sastavu, strukturi i osobinama kompleksa su nekompletni i ponekad kontradiktorni. Stoga je namera ovog rada da kroz retrospektivu naših rezultata damo lični doprinos u ispitivanju metal-flavonoid kompleksa. U prikazanom revijalnom radu sakupljeni su svi naši rezultati o približno 40 kompleksa obrazovanih između pet flavonoida (rutin, kvercetin, 3-hidroksiflavon, morin i hesperidin) i većeg broja metalnih jona ili metalnih grupa. Istaknuti su podaci o mestu vezivanja centralnog jona u kompleksu, stehiometrijskom odnosu metal-ligand, uticaju pH na formiranje kompleksa i konstantama stabilnosti kompleksa. Takođe, u ovom radu prikazali smo i primenu metal-flavonoid kompleksa u analitičke svrhe. .
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Investigation of metal-flavonoid chelates and the determination of flavonoids via metal-flavonoid complexing reactions
T1  - Ispitivanje metal-flavonoid helata i određivanje flavonoida preko kompleksirajuće reakcije metal-flavonoid
VL  - 72
IS  - 10
SP  - 921
EP  - 939
DO  - 10.2298/JSC0710921M
ER  - 

@article{
author = "Malešev, Dušan and Kuntić, Vesna",
year = "2007",
abstract = "Flavonoids constitute a large group of polyphenolic phytochemicals with antioxidant properties which are overwhelmingly exerted through direct free radical scavenging. Flavonoids also exhibit antioxidant properties through chelating with transition metals, primarily Fe(II), Fe(III) and Cu(II), which participate in reactions generating free radicals. Metal-flavonoid chelates are considerably more potent free radical scavengers than the parent flavonoids and play a prominent role in protecting from oxidative stress. To unravel the origin of their potent biological action extensive physico-chemical studies were undertaken to reveal the chemical structure, chelation sites, assess the impact of the metal/ligand ratio on the structure of the complexes and the capacity of flavonoids to bind metal ions. In spite of such extensive efforts, data on the composition, structure and complex-formation properties are incomplete and sometimes even contradictory. The aim of this paper is to give a personal account on the development of the field through a retrospective evaluation of our own research which covers approximately 40 complexes of flavonoids from different flavonoids subclasses (rutin, quercetin, 3-hydroxyflavone, morin and hesperidin) with several metal ions or groups and suggest directions for future research. Special emphasis will be given to the site of the central ion, the composition of the complexes, the role of pH in complex formation, the stability of metal-flavonoid complexes and their potential application for analytical purposes. ., Flavonoidi predstavljaju veliku grupu polifenolnih jedinjenja biljnog porekla, koji imaju antioksidantna svojstva zbog direktnog "hvatanja" slobodnih radikala. Antioksidantno dejstvo flavonoidi takođe ostvaruju stvaranjem helata sa jonima prelaznih metala, prvenstveno sa jonima Fe(II), Fe(III) i Cu(II), koji sami učestvuju u reakciji stvaranja slobodnih radikala. Helati metal-flavonoid su mnogo efikasniji "hvatači" slobodnih radikala od matičnih flavonoida i mogu zaštititi potencijalne mete u organizmu od oksidativnog stresa. Da bi se razjasnio mehanizam biološkog delovanja kompleksa metal-flavonoid, ispitivane su fizičko-hemijske karakteristike kompleksa: mesto vezivanja jona metala, zavisnost strukture kompleksa od odnosa metal/ligand, afinitet flavonoida za vezivanje metalnog jona, itd. Međutim, podaci o sastavu, strukturi i osobinama kompleksa su nekompletni i ponekad kontradiktorni. Stoga je namera ovog rada da kroz retrospektivu naših rezultata damo lični doprinos u ispitivanju metal-flavonoid kompleksa. U prikazanom revijalnom radu sakupljeni su svi naši rezultati o približno 40 kompleksa obrazovanih između pet flavonoida (rutin, kvercetin, 3-hidroksiflavon, morin i hesperidin) i većeg broja metalnih jona ili metalnih grupa. Istaknuti su podaci o mestu vezivanja centralnog jona u kompleksu, stehiometrijskom odnosu metal-ligand, uticaju pH na formiranje kompleksa i konstantama stabilnosti kompleksa. Takođe, u ovom radu prikazali smo i primenu metal-flavonoid kompleksa u analitičke svrhe. .",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Investigation of metal-flavonoid chelates and the determination of flavonoids via metal-flavonoid complexing reactions, Ispitivanje metal-flavonoid helata i određivanje flavonoida preko kompleksirajuće reakcije metal-flavonoid",
volume = "72",
number = "10",
pages = "921-939",
doi = "10.2298/JSC0710921M"
}

Malešev, D.,& Kuntić, V.. (2007). Investigation of metal-flavonoid chelates and the determination of flavonoids via metal-flavonoid complexing reactions. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 72(10), 921-939.
https://doi.org/10.2298/JSC0710921M

Malešev D, Kuntić V. Investigation of metal-flavonoid chelates and the determination of flavonoids via metal-flavonoid complexing reactions. in Journal of the Serbian Chemical Society. 2007;72(10):921-939.
doi:10.2298/JSC0710921M .

Malešev, Dušan, Kuntić, Vesna, "Investigation of metal-flavonoid chelates and the determination of flavonoids via metal-flavonoid complexing reactions" in Journal of the Serbian Chemical Society, 72, no. 10 (2007):921-939,
https://doi.org/10.2298/JSC0710921M . .

TY  - JOUR
AU  - Jelikić-Stankov, Milena
AU  - Uskoković-Marković, Snežana
AU  - Holclajtner-Antunović, Ivanka
AU  - Todorović, Marija R.
AU  - Đurđević, Predrag
PY  - 2007
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/912
AB  - Molybdenum, vanadium and tungsten compounds are widely applied as analytical reagents for determination of numerous pharmacologically active substances and different biochemical parameters. Recent data from the available literature pointed to a very potent biomedical activity of compounds containing these trace elements. The present paper represents a survey on the structure and chemical properties of these compounds, as well as on their biological activity, mostly based on their interaction with cations of biomolecules, such as phospholipids and proteins. Besides, their potent inhibitory effects on cellular targets, bacterial and viral DNA and RNA polymerases will be discussed, as well. Numerous authors clearly demonstrated the antiviral (especially anti-HIV), anticoagulant and antineoplastic properties of the compounds containing the above trace elements. It has been also shown that these compounds act on some cellular enzymatic systems leading to the normalisation of blood pressure, blood glucose and serum lipid levels. Also, compounds of these trace elements represent potent antiobesity agents and express hepatoprotective and antioxidative stress activity.
PB  - Elsevier Gmbh, Urban & Fischer Verlag, Jena
T2  - Journal of Trace Elements in Medicine and Biology
T1  - Compounds of Mo, V and W in biochemistry and their biomedical activity
VL  - 21
IS  - 1
SP  - 8
EP  - 16
DO  - 10.1016/j.jtemb.2006.11.004
ER  - 

@article{
author = "Jelikić-Stankov, Milena and Uskoković-Marković, Snežana and Holclajtner-Antunović, Ivanka and Todorović, Marija R. and Đurđević, Predrag",
year = "2007",
abstract = "Molybdenum, vanadium and tungsten compounds are widely applied as analytical reagents for determination of numerous pharmacologically active substances and different biochemical parameters. Recent data from the available literature pointed to a very potent biomedical activity of compounds containing these trace elements. The present paper represents a survey on the structure and chemical properties of these compounds, as well as on their biological activity, mostly based on their interaction with cations of biomolecules, such as phospholipids and proteins. Besides, their potent inhibitory effects on cellular targets, bacterial and viral DNA and RNA polymerases will be discussed, as well. Numerous authors clearly demonstrated the antiviral (especially anti-HIV), anticoagulant and antineoplastic properties of the compounds containing the above trace elements. It has been also shown that these compounds act on some cellular enzymatic systems leading to the normalisation of blood pressure, blood glucose and serum lipid levels. Also, compounds of these trace elements represent potent antiobesity agents and express hepatoprotective and antioxidative stress activity.",
publisher = "Elsevier Gmbh, Urban & Fischer Verlag, Jena",
journal = "Journal of Trace Elements in Medicine and Biology",
title = "Compounds of Mo, V and W in biochemistry and their biomedical activity",
volume = "21",
number = "1",
pages = "8-16",
doi = "10.1016/j.jtemb.2006.11.004"
}

Jelikić-Stankov, M., Uskoković-Marković, S., Holclajtner-Antunović, I., Todorović, M. R.,& Đurđević, P.. (2007). Compounds of Mo, V and W in biochemistry and their biomedical activity. in Journal of Trace Elements in Medicine and Biology
Elsevier Gmbh, Urban & Fischer Verlag, Jena., 21(1), 8-16.
https://doi.org/10.1016/j.jtemb.2006.11.004

Jelikić-Stankov M, Uskoković-Marković S, Holclajtner-Antunović I, Todorović MR, Đurđević P. Compounds of Mo, V and W in biochemistry and their biomedical activity. in Journal of Trace Elements in Medicine and Biology. 2007;21(1):8-16.
doi:10.1016/j.jtemb.2006.11.004 .

Jelikić-Stankov, Milena, Uskoković-Marković, Snežana, Holclajtner-Antunović, Ivanka, Todorović, Marija R., Đurđević, Predrag, "Compounds of Mo, V and W in biochemistry and their biomedical activity" in Journal of Trace Elements in Medicine and Biology, 21, no. 1 (2007):8-16,
https://doi.org/10.1016/j.jtemb.2006.11.004 . .