TY  - JOUR
AU  - Gandhi, Jarel K.
AU  - Živković, Lada
AU  - Fisher, John P.
AU  - Yoder, Mervin C.
AU  - Brey, Eric M.
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2328
AB  - Enhanced vascularization at sensor interfaces can improve long-term function. Fibrin, a natural polymer, has shown promise as a biomaterial for sensor coating due to its ability to sustain endothelial cell growth and promote local vascularization. However, the culture of cells, particularly endothelial cells (EC), within 3D scaffolds for more than a few days is challenging due to rapid loss of EC viability. In this manuscript, a robust method for developing fibrin microbead scaffolds for long-term culture of encapsulated ECs is described. Fibrin microbeads are formed using sodium alginate as a structural template. The size, swelling and structural properties of the microbeads were varied with needle gauge and composition and concentration of the pre-gel solution. Endothelial colony-forming cells (ECFCs) were suspended in the fibrin beads and cultured within a perfusion bioreactor system. The perfusion bioreactor enhanced ECFCs viability and genome stability in fibrin beads relative to static culture. Perfusion bioreactors enable 3D culture of ECs within fibrin beads for potential application as a sensor coating.
PB  - MDPI, Basel
T2  - Separation Science and Technology
T1  - Enhanced Viability of Endothelial Colony Forming Cells in Fibrin Microbeads for Sensor Vascularization
VL  - 15
IS  - 9
SP  - 23886
EP  - 23902
DO  - 10.3390/s150923886
ER  - 

@article{
author = "Gandhi, Jarel K. and Živković, Lada and Fisher, John P. and Yoder, Mervin C. and Brey, Eric M.",
year = "2015",
abstract = "Enhanced vascularization at sensor interfaces can improve long-term function. Fibrin, a natural polymer, has shown promise as a biomaterial for sensor coating due to its ability to sustain endothelial cell growth and promote local vascularization. However, the culture of cells, particularly endothelial cells (EC), within 3D scaffolds for more than a few days is challenging due to rapid loss of EC viability. In this manuscript, a robust method for developing fibrin microbead scaffolds for long-term culture of encapsulated ECs is described. Fibrin microbeads are formed using sodium alginate as a structural template. The size, swelling and structural properties of the microbeads were varied with needle gauge and composition and concentration of the pre-gel solution. Endothelial colony-forming cells (ECFCs) were suspended in the fibrin beads and cultured within a perfusion bioreactor system. The perfusion bioreactor enhanced ECFCs viability and genome stability in fibrin beads relative to static culture. Perfusion bioreactors enable 3D culture of ECs within fibrin beads for potential application as a sensor coating.",
publisher = "MDPI, Basel",
journal = "Separation Science and Technology",
title = "Enhanced Viability of Endothelial Colony Forming Cells in Fibrin Microbeads for Sensor Vascularization",
volume = "15",
number = "9",
pages = "23886-23902",
doi = "10.3390/s150923886"
}

Gandhi, J. K., Živković, L., Fisher, J. P., Yoder, M. C.,& Brey, E. M.. (2015). Enhanced Viability of Endothelial Colony Forming Cells in Fibrin Microbeads for Sensor Vascularization. in Separation Science and Technology
MDPI, Basel., 15(9), 23886-23902.
https://doi.org/10.3390/s150923886

Gandhi JK, Živković L, Fisher JP, Yoder MC, Brey EM. Enhanced Viability of Endothelial Colony Forming Cells in Fibrin Microbeads for Sensor Vascularization. in Separation Science and Technology. 2015;15(9):23886-23902.
doi:10.3390/s150923886 .

Gandhi, Jarel K., Živković, Lada, Fisher, John P., Yoder, Mervin C., Brey, Eric M., "Enhanced Viability of Endothelial Colony Forming Cells in Fibrin Microbeads for Sensor Vascularization" in Separation Science and Technology, 15, no. 9 (2015):23886-23902,
https://doi.org/10.3390/s150923886 . .