@conference{
author = "Dimitrijević, Aleksandra and Jocić, Ana and Marić, Slađana and Zdolšek, Nikola and Trtić-Petrović, Tatjana and Vraneš, Milan and Gadžurić, Slobodan and Arsenijević, Jelena and Ražić, Slavica and Tavares, Ana Paula M. and Freire, Mara G.",
year = "2019",
abstract = "The chemotherapeutic parthenolide (PTL) has an excellent
potential in the treatment of acute lymphoblastic leukemia and
much attention has been focused to overcome its poor aqueous
solubility, which limits PTL bioavailability. The most promising
approach is using micellar structures of Pluronics (class of triblock
copolymer of poly(ethylene)-poly(propylene)-poly(ethylene)),
as a drug delivery vectors. Pluronic PE6200 (PL6200) is selected
as one of the phase formers of aqueous biphasic system (ABS)
having in mind the amphiphilic nature of Pluronics which enables
good solubilization of hydrophobic molecules, their biocompatibility,
water solubility and complex mesophase behaviour. ABSs
are formed by combination of aqueous solutions of two polymers,
two salts or polymer/salt above certain concentrations with water
as the main constituent of both phases of ABS. In the quest for
more selective, performant and “greener” extraction technique of
PTL from natural matrices, a novel ABS comprised of a hydrophilic
ionic liquid (IL) - cholinium pyruvate (ChPyr) and PL6200
was investigated. In order to design selective Pluronic-mediated
purification process, (1) the ternary phase diagram was determinated
and characterised and (2) the partition of hydrophobic
PTL in this system was evaluated. A solution composed of 20
wt% of ChPyr + 30 wt% of PL6200 + 50 wt% of water with PTL
concentration of 2 mgml-1 led to high PTL extraction efficiency in
PL6200-rich phase (above 82%) with partition coefficient of 4.96.
The partition of PTL is mainly driven by its hydrophobic interaction
with poloxamer. For lower PL6200 concentrations in ABS,
formation of micelle particles was observed, what enabled solubilization
of PTL and its migration to hydrophilic IL-rich phase.
That decreased the PTL extraction efficiency toward PL6200-
rich phase. The obtained results showed that using cholinium-IL
/ Pluronic based ABS is promising alternative to volatile organic solvents that are nowdays used for extraction of PTL. Moreover,
using the Pluronic as constituent of ABS can provide the possibility
to simultaneously deal with low aqueous solubility of PTL by
forming the drug-loaded micelle particles and selective extraction
of active compound from real matrix.",
publisher = "Turkish Chemical Society",
journal = "XX Euroanalysis 2019 Conference, Abstracts and Proceedings",
title = "Controlling of the parthenolide partition using micelle structures of block copolymer in ionic liquid based aqueous biphasic system",
pages = "212-214",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5288"
}
