TY  - JOUR
AU  - Stanić, Vojislav
AU  - Janacković, Đorđe
AU  - Dimitrijević, Suzana
AU  - Tanasković, Slađana
AU  - Mitrić, Miodrag
AU  - Pavlović, Mirjana S.
AU  - Krstić, Aleksandra
AU  - Jovanović, Dragoljub
AU  - Raičević, Slavica
PY  - 2011
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1538
AB  - Monophase silver-doped hydroxyapatite (AgxCa10-x(PO4)(6)(OH)(2); 0.002  lt = x  lt = 0.04) nanoparticles were prepared using a neutralization method and investigated with respect to potential medical applications. This method consists of dissolving Ag2O in solution of H3PO4, and the slow addition to suspension of Ca(OH)(2) was applied for the purpose of homogenous distribution of silver ions. Characterization studies from XRD, TEM and FTIR spectra showed that obtained crystals are monophase hydroxyapatites and that particles of all samples are of nano size, with average length of 70nm and about 15-25nm in diameter. Antimicrobial studies have demonstrated that all silver-doped hydroxyapatite samples exhibit excellent antimicrobial activity in vitro against the following pathogens: Staphylococcus aureus, Escherichia coli and Candida albicans. The hydroxyapatite sample with the highest content of silver has shown the highest antimicrobial activity; killed all cells of E. coli and brought to more than 99% reduction in viable counts of S. aureus and C. albicans. The atomic force microscopic studies illustrate that silver-doped hydroxyapatite sample causes considerable morphological changes of microorganism cells which might be the cause of cells' death. Hemolysis ratios of the silver-doped hydroxyapatite samples were below 3%, indicating good blood compatibility and that are promising as biomaterials. Crown Copyright
PB  - Elsevier Science BV, Amsterdam
T2  - Applied Surface Science
T1  - Synthesis of antimicrobial monophase silver-doped hydroxyapatite nanopowders for bone tissue engineering
VL  - 257
IS  - 9
SP  - 4510
EP  - 4518
DO  - 10.1016/j.apsusc.2010.12.113
ER  - 

@article{
author = "Stanić, Vojislav and Janacković, Đorđe and Dimitrijević, Suzana and Tanasković, Slađana and Mitrić, Miodrag and Pavlović, Mirjana S. and Krstić, Aleksandra and Jovanović, Dragoljub and Raičević, Slavica",
year = "2011",
abstract = "Monophase silver-doped hydroxyapatite (AgxCa10-x(PO4)(6)(OH)(2); 0.002  lt = x  lt = 0.04) nanoparticles were prepared using a neutralization method and investigated with respect to potential medical applications. This method consists of dissolving Ag2O in solution of H3PO4, and the slow addition to suspension of Ca(OH)(2) was applied for the purpose of homogenous distribution of silver ions. Characterization studies from XRD, TEM and FTIR spectra showed that obtained crystals are monophase hydroxyapatites and that particles of all samples are of nano size, with average length of 70nm and about 15-25nm in diameter. Antimicrobial studies have demonstrated that all silver-doped hydroxyapatite samples exhibit excellent antimicrobial activity in vitro against the following pathogens: Staphylococcus aureus, Escherichia coli and Candida albicans. The hydroxyapatite sample with the highest content of silver has shown the highest antimicrobial activity; killed all cells of E. coli and brought to more than 99% reduction in viable counts of S. aureus and C. albicans. The atomic force microscopic studies illustrate that silver-doped hydroxyapatite sample causes considerable morphological changes of microorganism cells which might be the cause of cells' death. Hemolysis ratios of the silver-doped hydroxyapatite samples were below 3%, indicating good blood compatibility and that are promising as biomaterials. Crown Copyright",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Applied Surface Science",
title = "Synthesis of antimicrobial monophase silver-doped hydroxyapatite nanopowders for bone tissue engineering",
volume = "257",
number = "9",
pages = "4510-4518",
doi = "10.1016/j.apsusc.2010.12.113"
}

Stanić, V., Janacković, Đ., Dimitrijević, S., Tanasković, S., Mitrić, M., Pavlović, M. S., Krstić, A., Jovanović, D.,& Raičević, S.. (2011). Synthesis of antimicrobial monophase silver-doped hydroxyapatite nanopowders for bone tissue engineering. in Applied Surface Science
Elsevier Science BV, Amsterdam., 257(9), 4510-4518.
https://doi.org/10.1016/j.apsusc.2010.12.113

Stanić V, Janacković Đ, Dimitrijević S, Tanasković S, Mitrić M, Pavlović MS, Krstić A, Jovanović D, Raičević S. Synthesis of antimicrobial monophase silver-doped hydroxyapatite nanopowders for bone tissue engineering. in Applied Surface Science. 2011;257(9):4510-4518.
doi:10.1016/j.apsusc.2010.12.113 .

Stanić, Vojislav, Janacković, Đorđe, Dimitrijević, Suzana, Tanasković, Slađana, Mitrić, Miodrag, Pavlović, Mirjana S., Krstić, Aleksandra, Jovanović, Dragoljub, Raičević, Slavica, "Synthesis of antimicrobial monophase silver-doped hydroxyapatite nanopowders for bone tissue engineering" in Applied Surface Science, 257, no. 9 (2011):4510-4518,
https://doi.org/10.1016/j.apsusc.2010.12.113 . .

TY  - JOUR
AU  - Kotur-Stevuljević, Jelena
AU  - Spasić, Slavica
AU  - Jelić-Ivanović, Zorana
AU  - Spasojević-Kalimanovska, Vesna
AU  - Stefanović, Aleksandra
AU  - Vujović, Ana
AU  - Memon, Lidija
AU  - Kalimanovska-Oštrić, Dimitra
PY  - 2008
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1119
AB  - Objectives: High-density lipoprotein (HDL) associated paraoxonase. 1 (PON1) is an essential component of HDLs' capability to protect low-density lipoproteins (LDL) from oxidative modification and thus to limit the atherosclerotic process. The aim of the Current Study was to investigate the association between oxidative stress status, indices of inflammation and PON1 status parameters. Design and methods: We determined the relationship between the oxidative stress status, inflammatory markers and PON1 status parameters in 261 middle-aged subjects: 156 coronary heart disease (CHD) patients and 105 CHD-free subjects (as the control group). The PON1 status involved PON1 activity measurements towards two substrates: paraoxon (POase activity) and diazoxon (DZOase activity) and subsequent PON1(Q192R) activity phenotype determination. Results: A statistically significant greater malondialdehyde (MDA) concentration in the RR phenotype subjects compared to QQ subjects within the CHD group was apparent (P lt 0.05). Multiple linear regression analysis revealed an independent influence of plasmatic SOD activity (P lt 0.05) on POase values and MDA (P lt 0.01) and O-2(center dot-) (P lt 0.05) on DZOase values. Involvement of inflammatory markers (fibrinogen and hsCRP) in the regression model did not hinder the influence of SOD and MDA on POase and DZOase activities, respectively. Conclusions: Our CHD patients were in a state of oxidative stress, which was most evident in the RR phenotype group. The QQ phenotype group is associated with the lowest oxidative stress status level and also with a better capacity for anti-oxidative protection. Oxidative stress in CHD patients is maintained by systemic low-grade inflammation which results in PON1 enzymatic activity exhaustion. Therefore, deeper investigation of an effective anti-oxidative and anti-inflammatory therapy should be necessary in order to increase anti-oxidative potency and improve PON1 status of CHD patients.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Clinical Biochemistry
T1  - PON1 status is influenced by oxidative stress and inflammation in coronary heart disease patients
VL  - 41
IS  - 13
SP  - 1067
EP  - 1073
DO  - 10.1016/j.clinbiochem.2008.06.009
ER  - 

@article{
author = "Kotur-Stevuljević, Jelena and Spasić, Slavica and Jelić-Ivanović, Zorana and Spasojević-Kalimanovska, Vesna and Stefanović, Aleksandra and Vujović, Ana and Memon, Lidija and Kalimanovska-Oštrić, Dimitra",
year = "2008",
abstract = "Objectives: High-density lipoprotein (HDL) associated paraoxonase. 1 (PON1) is an essential component of HDLs' capability to protect low-density lipoproteins (LDL) from oxidative modification and thus to limit the atherosclerotic process. The aim of the Current Study was to investigate the association between oxidative stress status, indices of inflammation and PON1 status parameters. Design and methods: We determined the relationship between the oxidative stress status, inflammatory markers and PON1 status parameters in 261 middle-aged subjects: 156 coronary heart disease (CHD) patients and 105 CHD-free subjects (as the control group). The PON1 status involved PON1 activity measurements towards two substrates: paraoxon (POase activity) and diazoxon (DZOase activity) and subsequent PON1(Q192R) activity phenotype determination. Results: A statistically significant greater malondialdehyde (MDA) concentration in the RR phenotype subjects compared to QQ subjects within the CHD group was apparent (P lt 0.05). Multiple linear regression analysis revealed an independent influence of plasmatic SOD activity (P lt 0.05) on POase values and MDA (P lt 0.01) and O-2(center dot-) (P lt 0.05) on DZOase values. Involvement of inflammatory markers (fibrinogen and hsCRP) in the regression model did not hinder the influence of SOD and MDA on POase and DZOase activities, respectively. Conclusions: Our CHD patients were in a state of oxidative stress, which was most evident in the RR phenotype group. The QQ phenotype group is associated with the lowest oxidative stress status level and also with a better capacity for anti-oxidative protection. Oxidative stress in CHD patients is maintained by systemic low-grade inflammation which results in PON1 enzymatic activity exhaustion. Therefore, deeper investigation of an effective anti-oxidative and anti-inflammatory therapy should be necessary in order to increase anti-oxidative potency and improve PON1 status of CHD patients.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Clinical Biochemistry",
title = "PON1 status is influenced by oxidative stress and inflammation in coronary heart disease patients",
volume = "41",
number = "13",
pages = "1067-1073",
doi = "10.1016/j.clinbiochem.2008.06.009"
}

Kotur-Stevuljević, J., Spasić, S., Jelić-Ivanović, Z., Spasojević-Kalimanovska, V., Stefanović, A., Vujović, A., Memon, L.,& Kalimanovska-Oštrić, D.. (2008). PON1 status is influenced by oxidative stress and inflammation in coronary heart disease patients. in Clinical Biochemistry
Pergamon-Elsevier Science Ltd, Oxford., 41(13), 1067-1073.
https://doi.org/10.1016/j.clinbiochem.2008.06.009

Kotur-Stevuljević J, Spasić S, Jelić-Ivanović Z, Spasojević-Kalimanovska V, Stefanović A, Vujović A, Memon L, Kalimanovska-Oštrić D. PON1 status is influenced by oxidative stress and inflammation in coronary heart disease patients. in Clinical Biochemistry. 2008;41(13):1067-1073.
doi:10.1016/j.clinbiochem.2008.06.009 .

Kotur-Stevuljević, Jelena, Spasić, Slavica, Jelić-Ivanović, Zorana, Spasojević-Kalimanovska, Vesna, Stefanović, Aleksandra, Vujović, Ana, Memon, Lidija, Kalimanovska-Oštrić, Dimitra, "PON1 status is influenced by oxidative stress and inflammation in coronary heart disease patients" in Clinical Biochemistry, 41, no. 13 (2008):1067-1073,
https://doi.org/10.1016/j.clinbiochem.2008.06.009 . .