TY  - JOUR
AU  - Milanović, Zorana
AU  - Beletić, Anđelo
AU  - Vekić, Jelena
AU  - Zeljković, Aleksandra
AU  - Andrić, Nenad
AU  - Ilić Božović, Anja
AU  - Spariosu, Kristina
AU  - Radaković, Milena
AU  - Ajtić, Jelena
AU  - Kovačević Filipović, Milica
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3588
AB  - Asymptomatic outdoor dogs can be carriers of Babesia canis, but data describing the development of an acute phase response (APR) are not available. We hypothesised that these dogs have a moderate APR that could be detected by hematological and biochemical changes. Two groups of Babesia-exposed dogs were represented by nine B. canis PCR-positive and twenty B. canis PCR-negative, seroreactive dogs. The control group consisted of ten Babesia-naïve dogs. Serum amyloid A (SAA), paraoxonase-1 (PON-1), complete blood count, and biochemistry parameters were analysed by standard methodologies. Protein and lipoprotein fractions were separated using agarose gel electrophoresis (GE), and the dominant diameters of lipoproteins were assessed on gradient GE. Results were evaluated using non-parametric tests and the Receiver Operating Characteristic curve. SAA (median 39.0 μg/mL, range 2.2–48.8 μg/mL), total protein (median 74.7 g/L, range 57.1–98.3 g/L) and the dominant diameter of α-lipoproteins (median 13.31 nm, range 12.09–14.17 nm) in B. canis PCR-positive dogs were higher relative to dogs in the control group or dogs that were PCR-negative but seroreactive (p < 0.001 for both groups). Mild to moderate anemia (4/29), thrombocytopenia (7/29), and leukocyte counts that were close to the upper limit of the reference range were encountered in both Babesia-exposed groups. When compared to controls, Babesia-exposed dogs displayed decreased a PON-1 activity and protein GE pattern consistent with low-grade chronic inflammation (p < 0.001 for both groups). Dogs with detectable amounts of B. canis DNA in blood contain increased levels of SAA and total protein along with α-lipoproteins that display an increased diameter relative to those dogs with positive Babesia serology but undetectable levels of B. canis DNA in blood.
PB  - Elsevier
T2  - Veterinary Parasitology
T1  - Evidence of acute phase reaction in asymptomatic dogs naturally infected with Babesia canis
VL  - 282
DO  - 10.1016/j.vetpar.2020.109140
ER  - 

@article{
author = "Milanović, Zorana and Beletić, Anđelo and Vekić, Jelena and Zeljković, Aleksandra and Andrić, Nenad and Ilić Božović, Anja and Spariosu, Kristina and Radaković, Milena and Ajtić, Jelena and Kovačević Filipović, Milica",
year = "2020",
abstract = "Asymptomatic outdoor dogs can be carriers of Babesia canis, but data describing the development of an acute phase response (APR) are not available. We hypothesised that these dogs have a moderate APR that could be detected by hematological and biochemical changes. Two groups of Babesia-exposed dogs were represented by nine B. canis PCR-positive and twenty B. canis PCR-negative, seroreactive dogs. The control group consisted of ten Babesia-naïve dogs. Serum amyloid A (SAA), paraoxonase-1 (PON-1), complete blood count, and biochemistry parameters were analysed by standard methodologies. Protein and lipoprotein fractions were separated using agarose gel electrophoresis (GE), and the dominant diameters of lipoproteins were assessed on gradient GE. Results were evaluated using non-parametric tests and the Receiver Operating Characteristic curve. SAA (median 39.0 μg/mL, range 2.2–48.8 μg/mL), total protein (median 74.7 g/L, range 57.1–98.3 g/L) and the dominant diameter of α-lipoproteins (median 13.31 nm, range 12.09–14.17 nm) in B. canis PCR-positive dogs were higher relative to dogs in the control group or dogs that were PCR-negative but seroreactive (p < 0.001 for both groups). Mild to moderate anemia (4/29), thrombocytopenia (7/29), and leukocyte counts that were close to the upper limit of the reference range were encountered in both Babesia-exposed groups. When compared to controls, Babesia-exposed dogs displayed decreased a PON-1 activity and protein GE pattern consistent with low-grade chronic inflammation (p < 0.001 for both groups). Dogs with detectable amounts of B. canis DNA in blood contain increased levels of SAA and total protein along with α-lipoproteins that display an increased diameter relative to those dogs with positive Babesia serology but undetectable levels of B. canis DNA in blood.",
publisher = "Elsevier",
journal = "Veterinary Parasitology",
title = "Evidence of acute phase reaction in asymptomatic dogs naturally infected with Babesia canis",
volume = "282",
doi = "10.1016/j.vetpar.2020.109140"
}

Milanović, Z., Beletić, A., Vekić, J., Zeljković, A., Andrić, N., Ilić Božović, A., Spariosu, K., Radaković, M., Ajtić, J.,& Kovačević Filipović, M.. (2020). Evidence of acute phase reaction in asymptomatic dogs naturally infected with Babesia canis. in Veterinary Parasitology
Elsevier., 282.
https://doi.org/10.1016/j.vetpar.2020.109140

Milanović Z, Beletić A, Vekić J, Zeljković A, Andrić N, Ilić Božović A, Spariosu K, Radaković M, Ajtić J, Kovačević Filipović M. Evidence of acute phase reaction in asymptomatic dogs naturally infected with Babesia canis. in Veterinary Parasitology. 2020;282.
doi:10.1016/j.vetpar.2020.109140 .

Milanović, Zorana, Beletić, Anđelo, Vekić, Jelena, Zeljković, Aleksandra, Andrić, Nenad, Ilić Božović, Anja, Spariosu, Kristina, Radaković, Milena, Ajtić, Jelena, Kovačević Filipović, Milica, "Evidence of acute phase reaction in asymptomatic dogs naturally infected with Babesia canis" in Veterinary Parasitology, 282 (2020),
https://doi.org/10.1016/j.vetpar.2020.109140 . .

TY  - JOUR
AU  - Milanović, Z
AU  - Vekić, Jelena
AU  - Radonjić, V
AU  - Ilić-Božović, A
AU  - Zeljković, Aleksandra
AU  - Janać, Jelena
AU  - Spasojević-Kalimanovska, Vesna
AU  - Buch, J
AU  - Chandrashekar, R
AU  - Bojić-Trbojević, Ž
AU  - Hajduković, L
AU  - Christopher, M.M
AU  - Kovačević Filipović, M
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3253
AB  - Background: Babesia canis infection induces a marked acute phase response (APR) that might be associated with alteration in lipid and lipoprotein metabolism and disease prognosis. Hypothesis: Dogs with B. canis-induced APR develop dyslipidemia with altered lipoprotein concentration and morphology. Animals: Twenty-nine client-owned dogs with acute B. canis infection and 10 clinically healthy control dogs. Methods: Observational cross-sectional study. Serum amyloid A (SAA) was measured using ELISA. Cholesterol, phospholipids, and triglycerides were determined biochemically. Lipoproteins were separated using agarose gel electrophoresis. Lipoprotein diameter was assessed by polyacrylamide gradient gel electrophoresis; correlation with ApoA-1 (radioimmunoassay) and SAA was determined. Results: Dogs with B. canis infection had a marked APR (median SAA, 168.3 μg/mL; range, 98.1-716.2 μg/mL) compared with controls (3.2 μg/mL, 2.0-4.2 μg/mL) (P  lt .001). Dogs with B. canis infection had significantly lower median cholesterol (4.79 mmol/L, 1.89-7.64 mmol/L versus 6.15 mmol/L, 4.2-7.4 mmol/L) (P =.02), phospholipid (4.64 mmol/L, 2.6-6.6 mmol/L versus 5.72 mmol/L, 4.68-7.0 mmol/L) (P =.02), and α-lipoproteins (77.5%, 27.7%-93.5% versus 89.2%, 75.1%-93.5%) (P =.04), and higher ApoA-1 (1.36 U, 0.8-2.56 U versus 0.95 U, 0.73-1.54 U) concentrations (P =.02). Serum amyloid A correlated with high-density lipoproteins (HDLs) diameter (rho =.43; P =.03) and ApoA-1 (rho =.63, P  lt .001). Conclusions and Clinical Importance: Major changes associated with B. canis-induced APR in dogs are related to concentration, composition, and morphology of HDL particles pointing to an altered reverse cholesterol transport. Parallel ApoA-1 and SAA concentration increase is a unique still unexplained pathophysiological finding.
PB  - Blackwell Publishing Inc.
T2  - Journal of Veterinary Internal Medicine
T1  - Association of acute Babesia canis infection and serum lipid, lipoprotein, and apoprotein concentrations in dogs
VL  - 33
IS  - 4
SP  - 1686
EP  - 1694
DO  - 10.1111/jvim.15537
ER  - 

@article{
author = "Milanović, Z and Vekić, Jelena and Radonjić, V and Ilić-Božović, A and Zeljković, Aleksandra and Janać, Jelena and Spasojević-Kalimanovska, Vesna and Buch, J and Chandrashekar, R and Bojić-Trbojević, Ž and Hajduković, L and Christopher, M.M and Kovačević Filipović, M",
year = "2019",
abstract = "Background: Babesia canis infection induces a marked acute phase response (APR) that might be associated with alteration in lipid and lipoprotein metabolism and disease prognosis. Hypothesis: Dogs with B. canis-induced APR develop dyslipidemia with altered lipoprotein concentration and morphology. Animals: Twenty-nine client-owned dogs with acute B. canis infection and 10 clinically healthy control dogs. Methods: Observational cross-sectional study. Serum amyloid A (SAA) was measured using ELISA. Cholesterol, phospholipids, and triglycerides were determined biochemically. Lipoproteins were separated using agarose gel electrophoresis. Lipoprotein diameter was assessed by polyacrylamide gradient gel electrophoresis; correlation with ApoA-1 (radioimmunoassay) and SAA was determined. Results: Dogs with B. canis infection had a marked APR (median SAA, 168.3 μg/mL; range, 98.1-716.2 μg/mL) compared with controls (3.2 μg/mL, 2.0-4.2 μg/mL) (P  lt .001). Dogs with B. canis infection had significantly lower median cholesterol (4.79 mmol/L, 1.89-7.64 mmol/L versus 6.15 mmol/L, 4.2-7.4 mmol/L) (P =.02), phospholipid (4.64 mmol/L, 2.6-6.6 mmol/L versus 5.72 mmol/L, 4.68-7.0 mmol/L) (P =.02), and α-lipoproteins (77.5%, 27.7%-93.5% versus 89.2%, 75.1%-93.5%) (P =.04), and higher ApoA-1 (1.36 U, 0.8-2.56 U versus 0.95 U, 0.73-1.54 U) concentrations (P =.02). Serum amyloid A correlated with high-density lipoproteins (HDLs) diameter (rho =.43; P =.03) and ApoA-1 (rho =.63, P  lt .001). Conclusions and Clinical Importance: Major changes associated with B. canis-induced APR in dogs are related to concentration, composition, and morphology of HDL particles pointing to an altered reverse cholesterol transport. Parallel ApoA-1 and SAA concentration increase is a unique still unexplained pathophysiological finding.",
publisher = "Blackwell Publishing Inc.",
journal = "Journal of Veterinary Internal Medicine",
title = "Association of acute Babesia canis infection and serum lipid, lipoprotein, and apoprotein concentrations in dogs",
volume = "33",
number = "4",
pages = "1686-1694",
doi = "10.1111/jvim.15537"
}

Milanović, Z., Vekić, J., Radonjić, V., Ilić-Božović, A., Zeljković, A., Janać, J., Spasojević-Kalimanovska, V., Buch, J., Chandrashekar, R., Bojić-Trbojević, Ž., Hajduković, L., Christopher, M.M,& Kovačević Filipović, M.. (2019). Association of acute Babesia canis infection and serum lipid, lipoprotein, and apoprotein concentrations in dogs. in Journal of Veterinary Internal Medicine
Blackwell Publishing Inc.., 33(4), 1686-1694.
https://doi.org/10.1111/jvim.15537

Milanović Z, Vekić J, Radonjić V, Ilić-Božović A, Zeljković A, Janać J, Spasojević-Kalimanovska V, Buch J, Chandrashekar R, Bojić-Trbojević Ž, Hajduković L, Christopher M, Kovačević Filipović M. Association of acute Babesia canis infection and serum lipid, lipoprotein, and apoprotein concentrations in dogs. in Journal of Veterinary Internal Medicine. 2019;33(4):1686-1694.
doi:10.1111/jvim.15537 .

Milanović, Z, Vekić, Jelena, Radonjić, V, Ilić-Božović, A, Zeljković, Aleksandra, Janać, Jelena, Spasojević-Kalimanovska, Vesna, Buch, J, Chandrashekar, R, Bojić-Trbojević, Ž, Hajduković, L, Christopher, M.M, Kovačević Filipović, M, "Association of acute Babesia canis infection and serum lipid, lipoprotein, and apoprotein concentrations in dogs" in Journal of Veterinary Internal Medicine, 33, no. 4 (2019):1686-1694,
https://doi.org/10.1111/jvim.15537 . .