@article{
author = "Pavun, Leposava and Uskoković-Marković, Snežana",
year = "2019",
abstract = "Hesperidin is the flavonoid heteroside most found in citrus species, such
as grapefruit, lemon and orange. It is applied in the treatment of numerous
cardiovascular diseases, with many products containing hesperidin in
combination with other flavonoid heterosides or vitamin C. The aim of this
study was to develop and validate a simple, rapid and sensitive and lowcost
method for determination of hesperidin in tablets and orange juices.
The developed spectrophotometric method is based on the formation of
the Zn(II)-hesperidin complex in 70 v/v% methanol at pH 3.12, with max of
absorption at λmax= 283 nm. The method of variation of equimolar solutions
confirmed the stoichiometry of the complex as hesperidin: zinc= 2:1.
The stability constant of the complex was obtained by the Bjerrum method
and it is logβ2 = 17.01 at pH 3.12. Linearity range for the developed method
was defined as 0.61-7.32 μg/mL, (confirmed by coefficient of determination,
r2 = 0.9882), while limits of detection and quantification were calculated
as LOD = 0.15 μg/mL and LOQ = 0.45 μg/mL. Fairly high precision of the
method is indicated by low values of CV, varying in the range 0.26-1.23%.
The proposed method fulfills all aimed requirements, and it was successfully
applied for determination of hesperidin in tablets with recovery value 92.2
% and commercially available orange juices., Hesperidin je flavonoidni heterozid koji se najčešće nalazi u citrusnim vr-
stama, kao što su grejpfrut, limun i pomorandža. Primenjuje se u lečenju
brojnih kardiovaskularnih bolesti, sa mnogim proizvodima koji sadrže hes-
peridin u kombinaciji sa drugim flavonoidnim heterozidima ili vitaminom
C. Cilj ove studije je razvijanje i primena jednostavne, brze i osetljive, a pri
tome pristupačne metode za određivanje hesperidina u tabletama i sokovi-
ma od pomorandže. Razvijena spektrofotometrijska metoda zasniva se na
formiranju Zn(II)-hesperidin kompleksa u 70 v/v% metanolu pri pH 3,12, sa
maksimumom absorbancije na λ max= 283 nm. Metodom varijacije ekvimo-
larnih rastvora utvrđena je stehiometrija kompleksa hesperidin: cink = 2:1.
Konstanta stabilnosti kompleksa dobijena Bjerumovom metodom iznosi
logβ2 = 17,01 pri pH 3,12. Linearnost razvijene metode utvrđena je u oblasti
0,61 - 7,32 μg/mL, (potvrđeno koeficijentom određivanja, r2 = 0,9882), dok
su izračunati limiti detekcije i kvantifikacije LOD = 0,15 μg/mL i LOQ = 0,45
μg/mL. Na visoku preciznost metode ukazuju niske vrednosti koeficijenata
varijacije (CV je u oblasti 0,26 – 1,23 %). Predložena metoda ispunjava sve
postavljene zahteve i sa uspehom je primenjena za određivanje hesperidina
u tabletama uz recovery vrednost 92,2 % i komercijalno dostupnim sokovi-
ma od pomorandže.",
publisher = "Društvo za ishranu Srbije, Beograd",
journal = "Hrana i ishrana",
title = "Spectrophotometric determination of hesperidin in supplements and orange juices, Spektrofotometrijsko određivanje hesperidina u suplementima i
sokovima od pomorandže",
volume = "60",
number = "1",
pages = "18-23",
doi = "10.5937/HraIsh1901018P"
}