TY  - JOUR
AU  - Dragačević, Luka
AU  - Đorđević, Brižita
AU  - Gavrović-Jankulović, Marija
AU  - Ilić, Vesna
AU  - Kanazir, Danijela
AU  - Minić, Rajna
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3531
AB  - The surface of microorganisms is covered with polysaccharide structures which are in immediate contact with receptor structures on host’s cells and antibodies. The interaction between microorganisms and their host is dependent on surface glycosylation and in this study we have tested the interaction of plant lectins with different microorganisms. Enzyme-linked lectin sorbent assay - ELLSA was used to test the binding of recombinant Musa acuminata lectin - BL to 27 selected microorganisms and 7 other lectins were used for comparison: Soy bean agglutinin - SBA, Lens culinaris lectin - LCA, Wheat germ agglutinin - WGA, RCA120 - Ricinus communis agglutinin, Con A - from Canavalia ensiformis, Sambucus nigra agglutinin - SNA I and Maackia amurensis agglutinin - MAA. The goal was to define the microorganisms’ surface glycosylation by means of interaction with the selected plant lectins and to make a comparison with BL. Among the tested lectins most selective binding was observed for RCA120 which preferentially bound Lactobacillus casei DG. Recombinant banana lectin showed specific binding to all of the tested fungal species. The binding of BL to Candida albicans was further tested with fluorescence microscopy and flow cytometry and it was concluded that this lectin can differentiate ß-glucan rich surfaces. The binding of BL to S. boulardii could be inhibited with ß-glucan from yeast with IC50 1.81 μg mL−1 and to P. roqueforti with 1.10 μg mL−1. This unique specificity of BL could be exploited for screening purposes and potentially for the detection of ß-glucan in solutions.
PB  - Springer Nature
T2  - Glycoconjugate Journal
T1  - ELLSA based profiling of surface glycosylation in microorganisms reveals that ß-glucan rich yeasts’ surfaces are selectively recognized with recombinant banana lectin
VL  - 37
IS  - 1
SP  - 95
EP  - 105
DO  - 10.1007/s10719-019-09898-8
ER  - 

@article{
author = "Dragačević, Luka and Đorđević, Brižita and Gavrović-Jankulović, Marija and Ilić, Vesna and Kanazir, Danijela and Minić, Rajna",
year = "2020",
abstract = "The surface of microorganisms is covered with polysaccharide structures which are in immediate contact with receptor structures on host’s cells and antibodies. The interaction between microorganisms and their host is dependent on surface glycosylation and in this study we have tested the interaction of plant lectins with different microorganisms. Enzyme-linked lectin sorbent assay - ELLSA was used to test the binding of recombinant Musa acuminata lectin - BL to 27 selected microorganisms and 7 other lectins were used for comparison: Soy bean agglutinin - SBA, Lens culinaris lectin - LCA, Wheat germ agglutinin - WGA, RCA120 - Ricinus communis agglutinin, Con A - from Canavalia ensiformis, Sambucus nigra agglutinin - SNA I and Maackia amurensis agglutinin - MAA. The goal was to define the microorganisms’ surface glycosylation by means of interaction with the selected plant lectins and to make a comparison with BL. Among the tested lectins most selective binding was observed for RCA120 which preferentially bound Lactobacillus casei DG. Recombinant banana lectin showed specific binding to all of the tested fungal species. The binding of BL to Candida albicans was further tested with fluorescence microscopy and flow cytometry and it was concluded that this lectin can differentiate ß-glucan rich surfaces. The binding of BL to S. boulardii could be inhibited with ß-glucan from yeast with IC50 1.81 μg mL−1 and to P. roqueforti with 1.10 μg mL−1. This unique specificity of BL could be exploited for screening purposes and potentially for the detection of ß-glucan in solutions.",
publisher = "Springer Nature",
journal = "Glycoconjugate Journal",
title = "ELLSA based profiling of surface glycosylation in microorganisms reveals that ß-glucan rich yeasts’ surfaces are selectively recognized with recombinant banana lectin",
volume = "37",
number = "1",
pages = "95-105",
doi = "10.1007/s10719-019-09898-8"
}

Dragačević, L., Đorđević, B., Gavrović-Jankulović, M., Ilić, V., Kanazir, D.,& Minić, R.. (2020). ELLSA based profiling of surface glycosylation in microorganisms reveals that ß-glucan rich yeasts’ surfaces are selectively recognized with recombinant banana lectin. in Glycoconjugate Journal
Springer Nature., 37(1), 95-105.
https://doi.org/10.1007/s10719-019-09898-8

Dragačević L, Đorđević B, Gavrović-Jankulović M, Ilić V, Kanazir D, Minić R. ELLSA based profiling of surface glycosylation in microorganisms reveals that ß-glucan rich yeasts’ surfaces are selectively recognized with recombinant banana lectin. in Glycoconjugate Journal. 2020;37(1):95-105.
doi:10.1007/s10719-019-09898-8 .

Dragačević, Luka, Đorđević, Brižita, Gavrović-Jankulović, Marija, Ilić, Vesna, Kanazir, Danijela, Minić, Rajna, "ELLSA based profiling of surface glycosylation in microorganisms reveals that ß-glucan rich yeasts’ surfaces are selectively recognized with recombinant banana lectin" in Glycoconjugate Journal, 37, no. 1 (2020):95-105,
https://doi.org/10.1007/s10719-019-09898-8 . .

TY  - JOUR
AU  - Minić, Rajna
AU  - Papić, Zlatko
AU  - Đorđević, Brižita
AU  - Michalickova, Danica
AU  - Llić, Vesna
AU  - Mathiesen, Geir
AU  - Živković, Irena
AU  - Pantić, Višnja
AU  - Dimitrijević, Ljiljana
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3199
AB  - The goal of this work was to elucidate similarities between microorganisms from the perspective of the humoral immune system reactivity in professional athletes. The reactivity of serum IgG of 14 young, individuals was analyzed to 23 selected microorganisms as antigens by use of the in house ELISA. Serum IgM and IgA reactivity was also analyzed and a control group of sex and age matched individuals was used for comparison. The obtained absorbance levels were used as a string of values to correlate the reactivity to different microorganisms. IgM was found to be the most cross reactive antibody class, Pearson's r = 0.7 +/- 0.92, for very distant bacterial species such as Lactobacillus and E. coli. High correlation in IgG levels was found for Gammaproteobacteria and LPS (from E. coli) (r = 0.77 for LPS vs. P. aeruginosa to r = 0.98 for LPS vs. E. coli), whereas this correlation was lower in the control group (r = 0.49 for LPS vs. P. aeruginosa to r = 0.66 for LPS vs. E. coli). The correlation was also analyzed between total IgG and IgG subclasses specific for the same microorganism, and IgG2 was identified as the main subclass recognising different microorganisms, as well as recognising LPS. Upon correlation of IgG with IgA for the same microorganism absence of or negative correlation was found between bacteria-specific IgA and IgG in case of Lactobacillus and Staphylococcusgeni, whereas correlation was absent or positive for Candida albicans, Enterococcusfaecalis, Streptococcus species tested in professional athletes. Opposite results were obtained for the control group. Outlined here is a simple experimental procedure and data analysis which yields functional significance and which can be used for determining the similarities between microorganisms from the aspect of the humoral immune system, for determining the main IgG subclass involved in an immune response as well as for the analysis of different target populations.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - Profiling of microorganism-binding serum antibody specificities in professional athletes
VL  - 13
IS  - 9
DO  - 10.1371/journal.pone.0203665
ER  - 

@article{
author = "Minić, Rajna and Papić, Zlatko and Đorđević, Brižita and Michalickova, Danica and Llić, Vesna and Mathiesen, Geir and Živković, Irena and Pantić, Višnja and Dimitrijević, Ljiljana",
year = "2018",
abstract = "The goal of this work was to elucidate similarities between microorganisms from the perspective of the humoral immune system reactivity in professional athletes. The reactivity of serum IgG of 14 young, individuals was analyzed to 23 selected microorganisms as antigens by use of the in house ELISA. Serum IgM and IgA reactivity was also analyzed and a control group of sex and age matched individuals was used for comparison. The obtained absorbance levels were used as a string of values to correlate the reactivity to different microorganisms. IgM was found to be the most cross reactive antibody class, Pearson's r = 0.7 +/- 0.92, for very distant bacterial species such as Lactobacillus and E. coli. High correlation in IgG levels was found for Gammaproteobacteria and LPS (from E. coli) (r = 0.77 for LPS vs. P. aeruginosa to r = 0.98 for LPS vs. E. coli), whereas this correlation was lower in the control group (r = 0.49 for LPS vs. P. aeruginosa to r = 0.66 for LPS vs. E. coli). The correlation was also analyzed between total IgG and IgG subclasses specific for the same microorganism, and IgG2 was identified as the main subclass recognising different microorganisms, as well as recognising LPS. Upon correlation of IgG with IgA for the same microorganism absence of or negative correlation was found between bacteria-specific IgA and IgG in case of Lactobacillus and Staphylococcusgeni, whereas correlation was absent or positive for Candida albicans, Enterococcusfaecalis, Streptococcus species tested in professional athletes. Opposite results were obtained for the control group. Outlined here is a simple experimental procedure and data analysis which yields functional significance and which can be used for determining the similarities between microorganisms from the aspect of the humoral immune system, for determining the main IgG subclass involved in an immune response as well as for the analysis of different target populations.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "Profiling of microorganism-binding serum antibody specificities in professional athletes",
volume = "13",
number = "9",
doi = "10.1371/journal.pone.0203665"
}

Minić, R., Papić, Z., Đorđević, B., Michalickova, D., Llić, V., Mathiesen, G., Živković, I., Pantić, V.,& Dimitrijević, L.. (2018). Profiling of microorganism-binding serum antibody specificities in professional athletes. in PLoS One
Public Library Science, San Francisco., 13(9).
https://doi.org/10.1371/journal.pone.0203665

Minić R, Papić Z, Đorđević B, Michalickova D, Llić V, Mathiesen G, Živković I, Pantić V, Dimitrijević L. Profiling of microorganism-binding serum antibody specificities in professional athletes. in PLoS One. 2018;13(9).
doi:10.1371/journal.pone.0203665 .

Minić, Rajna, Papić, Zlatko, Đorđević, Brižita, Michalickova, Danica, Llić, Vesna, Mathiesen, Geir, Živković, Irena, Pantić, Višnja, Dimitrijević, Ljiljana, "Profiling of microorganism-binding serum antibody specificities in professional athletes" in PLoS One, 13, no. 9 (2018),
https://doi.org/10.1371/journal.pone.0203665 . .

TY  - JOUR
AU  - Ivanović, Nevena
AU  - Minić, Rajna
AU  - Đuričić, Ivana
AU  - Radojević-Skodrić, Sanja
AU  - Živković, Irena
AU  - Šobajić, Slađana
AU  - Đorđević, Brižita
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2770
AB  - Western life style, and high calorie diet in particular is causing major health problems such as insulin resistance, hepatic steatosis and heart disease in the modern age. High fat diet (HFD) induces similar changes in mice, such as increased body weight, hypercholesterolemia and accumulation of triglycerides in the liver. These changes can be ameliorated by the administration of some Lactobacillus species. The focus of this study was to analyze the fatty acid content of liver, heart and brain tissues of mice fed HFD and administered with either Lactobacillus plantarum WCFS1 or Lactobacillus rhamnosus LA68, and to analyze the fatty acid content of these organs after a two months washout period. The fatty acid composition of mouse liver tissue changed significantly due to probiotic administration during a 12 weeks HFD regime and active Lactobacillus administration had a slightly reversing effect toward the standard mouse diet group, but after the washout period these changes disappeared. The fatty acid composition of the heart and brain tissues was significantly changed in the HFD regime but probiotic administration had no significant influence on the fatty acid profile of these two organs. Upon the 8 weeks washout period the only remaining beneficial effect was the significantly lower mouse weight in the supplemented groups compared to the HFD group.
PB  - Royal Soc Chemistry, Cambridge
T2  - Food & Function
T1  - Active Lactobacillus rhamnosus LA68 or Lactobacillus plantarum WCFS1 administration positively influences liver fatty acid composition in mice on a HFD regime
VL  - 7
IS  - 6
SP  - 2840
EP  - 2848
DO  - 10.1039/c5fo01432h
ER  - 

@article{
author = "Ivanović, Nevena and Minić, Rajna and Đuričić, Ivana and Radojević-Skodrić, Sanja and Živković, Irena and Šobajić, Slađana and Đorđević, Brižita",
year = "2016",
abstract = "Western life style, and high calorie diet in particular is causing major health problems such as insulin resistance, hepatic steatosis and heart disease in the modern age. High fat diet (HFD) induces similar changes in mice, such as increased body weight, hypercholesterolemia and accumulation of triglycerides in the liver. These changes can be ameliorated by the administration of some Lactobacillus species. The focus of this study was to analyze the fatty acid content of liver, heart and brain tissues of mice fed HFD and administered with either Lactobacillus plantarum WCFS1 or Lactobacillus rhamnosus LA68, and to analyze the fatty acid content of these organs after a two months washout period. The fatty acid composition of mouse liver tissue changed significantly due to probiotic administration during a 12 weeks HFD regime and active Lactobacillus administration had a slightly reversing effect toward the standard mouse diet group, but after the washout period these changes disappeared. The fatty acid composition of the heart and brain tissues was significantly changed in the HFD regime but probiotic administration had no significant influence on the fatty acid profile of these two organs. Upon the 8 weeks washout period the only remaining beneficial effect was the significantly lower mouse weight in the supplemented groups compared to the HFD group.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Food & Function",
title = "Active Lactobacillus rhamnosus LA68 or Lactobacillus plantarum WCFS1 administration positively influences liver fatty acid composition in mice on a HFD regime",
volume = "7",
number = "6",
pages = "2840-2848",
doi = "10.1039/c5fo01432h"
}

Ivanović, N., Minić, R., Đuričić, I., Radojević-Skodrić, S., Živković, I., Šobajić, S.,& Đorđević, B.. (2016). Active Lactobacillus rhamnosus LA68 or Lactobacillus plantarum WCFS1 administration positively influences liver fatty acid composition in mice on a HFD regime. in Food & Function
Royal Soc Chemistry, Cambridge., 7(6), 2840-2848.
https://doi.org/10.1039/c5fo01432h

Ivanović N, Minić R, Đuričić I, Radojević-Skodrić S, Živković I, Šobajić S, Đorđević B. Active Lactobacillus rhamnosus LA68 or Lactobacillus plantarum WCFS1 administration positively influences liver fatty acid composition in mice on a HFD regime. in Food & Function. 2016;7(6):2840-2848.
doi:10.1039/c5fo01432h .

Ivanović, Nevena, Minić, Rajna, Đuričić, Ivana, Radojević-Skodrić, Sanja, Živković, Irena, Šobajić, Slađana, Đorđević, Brižita, "Active Lactobacillus rhamnosus LA68 or Lactobacillus plantarum WCFS1 administration positively influences liver fatty acid composition in mice on a HFD regime" in Food & Function, 7, no. 6 (2016):2840-2848,
https://doi.org/10.1039/c5fo01432h . .

TY  - THES
AU  - Ivanović, Nevena
PY  - 2016
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=4001
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:13471/bdef:Content/download
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:13587/bdef:Izvestaj/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=48325135
UR  - http://nardus.mpn.gov.rs/123456789/6949
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3389
AB  - Prema definiciji Svetske Zdravstvene Organizacije, probiotici su živi mikroorganizmi kojikada su konzumirani u adekvatnoj količini imaju povoljne efekte na zdravlje domaćina.Pod pojmom probiotskih bakterija misli se većinom na bakterije mlečne kiseline (BMK)koje obuhvataju uglavnom vrste iz rodova Lactobacillus i Bifidobacterium. Rastući je brojdokaza koji ukazuju da primena BMK može ispoljiti povoljne efekte u tretmanukomponenata metaboličkog sindroma, kao što su gojaznost, dijabetes tip 2, dislipidemije,nealkoholna masna bolest jetre (engl. Nonalcoholic Fatty Liver Disease, NAFLD). NAFLDobuhvata čitav spektar oboljenja, počev od hepatične steatoze preko nealkoholnogsteatohepatitisa do hepatične fibroze. Predstavlja najčešći uzrok ozbiljnih i hroničnihoboljenja jetre kod odraslih i kod dece u zapadnim zemljama, sa prevalencom od 20-30% uopštoj odrasloj populaciji, pri čemu prevalenca korelira sa povećanom incidencomgojaznosti i metaboličkog sindroma. Nedavno objavljena istraživanja ukazuju na ulogucrevne mikrobiote u razvoju steatoze i progresiji NAFLD-a, a s obzirom da probiotici mogudelovati na više nivoa na patogenezu oboljenja, a uz to su još i bezbedni i pristupačni, nijeiznenađujuće što LAB postaju sve privlačniji kao potencijalna dugoročna terapija NAFLDa.Kliničke i eksperimentalne studije sugerišu da se probiotici značajno razlikuju u efektimai mehanizmima dejstva a razlike postoje, ne samo između različitih vrsta, već i izmeđurazličitih soje iste vrste. Zbog toga je najvažniji cilj ove studije bio da se razvije modelNAFLD (stadijum steatoze) ishranom visokog sadržaja masti (VSM), a zatim da se ispita iizvrši poređenje dejstva oralne primene dva različita soja BMK, Lactobacillus rhamnosusLA68 i Lactobacillus plantarum WCFS1, na imunološke i metaboličke parametre, narazvoj steatoze, kao i na sastav masnih kiselina organa kod miševa soja C57BL/6 uuslovima VSM ishrane.Nakon 16 nedelja VSM ishrane, u kojoj je 41% energije poticao od masti, kod miševa sojaC57BL/6 došlo je do značajnog porasta telesne mase, i razvoja steatoze koju je pratioporemećaj lipidnih parametara i porast nivoa leptina, što odgovara početnoj fazi NAFLD(stadijum steatoze)...
AB  - According to the World Health Organisation's definition, probiotics are alivemicroorganisms which, when consumed in adequate quantities, have beneficial effects onhost's health. The term probiotics bacteria mostly refers to lactic acid bacteria (LAB)which include species from the genera Lactobacillus and Bifidobacteria. The increasingamount of evidence indicates that the usage of LAB can have positive effects on thetreatment of metabolic syndrome components, such as: obesity, type 2 diabetes,dyslipidemia, nonalcoholic fatty liver disease (NAFLD). NAFLD includes a whole spectarof diseases, starting from hepatic steatosis through nonalcoholic steatohepatitis to hepaticfibrosis which may further progress into ireversibile cirrhosis and hepatocellular carcinoma.NAFLD represents the most common cause of severe and chronic liver diseases in adultsand children in Western countres, with the prevelance correlating with increased incidenceof obesity and metabolic syndrome. Recently published research points to the role ofintestinal microbiota in the development of steatosis and progression of NAFLD.Probiotics, especially LAB, can have an influence on disease pathogenesis at multiplelevels and besides that they are safe and affordable. Taking into account all abovementionedreasons, it is not surprising that LAB are becoming more and more attractive aspotential long-term NAFLD therapy. Clinical and experimental studies suggest that LABdiffer significantly in the effects and mechanisms of action. Those differences are not onlyamong different species but also among different strains within the same species. Becauseof that, the most important goal of this study was to develop a model of NAFLD (steatosisstadium) with use of high fat diet (HFD) and then to examine and to compare the effects oforal administration of two different LAB species/strains, Lactobacillus rhamnosus LA68and Lactobacillus plantarum WCFS1, on immunological and metabolic parameters, onsteatosis development status, as well as on fatty acid composition of liver and brain inC57BL/6 mice under high fat diet regime...
PB  - Универзитет у Београду, Фармацеутски факултет
T2  - Универзитет у Београду
T1  - Uticaj oralne primene Lactobacillus rhamnosus LA68 i Lactobacillus plantarum WCFS1 na imunološke i metaboličke parametre miševa u uslovima eksperimentalno indukovane nealkoholne masne jetre
UR  - https://hdl.handle.net/21.15107/rcub_nardus_6949
ER  - 

@phdthesis{
author = "Ivanović, Nevena",
year = "2016",
abstract = "Prema definiciji Svetske Zdravstvene Organizacije, probiotici su živi mikroorganizmi kojikada su konzumirani u adekvatnoj količini imaju povoljne efekte na zdravlje domaćina.Pod pojmom probiotskih bakterija misli se većinom na bakterije mlečne kiseline (BMK)koje obuhvataju uglavnom vrste iz rodova Lactobacillus i Bifidobacterium. Rastući je brojdokaza koji ukazuju da primena BMK može ispoljiti povoljne efekte u tretmanukomponenata metaboličkog sindroma, kao što su gojaznost, dijabetes tip 2, dislipidemije,nealkoholna masna bolest jetre (engl. Nonalcoholic Fatty Liver Disease, NAFLD). NAFLDobuhvata čitav spektar oboljenja, počev od hepatične steatoze preko nealkoholnogsteatohepatitisa do hepatične fibroze. Predstavlja najčešći uzrok ozbiljnih i hroničnihoboljenja jetre kod odraslih i kod dece u zapadnim zemljama, sa prevalencom od 20-30% uopštoj odrasloj populaciji, pri čemu prevalenca korelira sa povećanom incidencomgojaznosti i metaboličkog sindroma. Nedavno objavljena istraživanja ukazuju na ulogucrevne mikrobiote u razvoju steatoze i progresiji NAFLD-a, a s obzirom da probiotici mogudelovati na više nivoa na patogenezu oboljenja, a uz to su još i bezbedni i pristupačni, nijeiznenađujuće što LAB postaju sve privlačniji kao potencijalna dugoročna terapija NAFLDa.Kliničke i eksperimentalne studije sugerišu da se probiotici značajno razlikuju u efektimai mehanizmima dejstva a razlike postoje, ne samo između različitih vrsta, već i izmeđurazličitih soje iste vrste. Zbog toga je najvažniji cilj ove studije bio da se razvije modelNAFLD (stadijum steatoze) ishranom visokog sadržaja masti (VSM), a zatim da se ispita iizvrši poređenje dejstva oralne primene dva različita soja BMK, Lactobacillus rhamnosusLA68 i Lactobacillus plantarum WCFS1, na imunološke i metaboličke parametre, narazvoj steatoze, kao i na sastav masnih kiselina organa kod miševa soja C57BL/6 uuslovima VSM ishrane.Nakon 16 nedelja VSM ishrane, u kojoj je 41% energije poticao od masti, kod miševa sojaC57BL/6 došlo je do značajnog porasta telesne mase, i razvoja steatoze koju je pratioporemećaj lipidnih parametara i porast nivoa leptina, što odgovara početnoj fazi NAFLD(stadijum steatoze)..., According to the World Health Organisation's definition, probiotics are alivemicroorganisms which, when consumed in adequate quantities, have beneficial effects onhost's health. The term probiotics bacteria mostly refers to lactic acid bacteria (LAB)which include species from the genera Lactobacillus and Bifidobacteria. The increasingamount of evidence indicates that the usage of LAB can have positive effects on thetreatment of metabolic syndrome components, such as: obesity, type 2 diabetes,dyslipidemia, nonalcoholic fatty liver disease (NAFLD). NAFLD includes a whole spectarof diseases, starting from hepatic steatosis through nonalcoholic steatohepatitis to hepaticfibrosis which may further progress into ireversibile cirrhosis and hepatocellular carcinoma.NAFLD represents the most common cause of severe and chronic liver diseases in adultsand children in Western countres, with the prevelance correlating with increased incidenceof obesity and metabolic syndrome. Recently published research points to the role ofintestinal microbiota in the development of steatosis and progression of NAFLD.Probiotics, especially LAB, can have an influence on disease pathogenesis at multiplelevels and besides that they are safe and affordable. Taking into account all abovementionedreasons, it is not surprising that LAB are becoming more and more attractive aspotential long-term NAFLD therapy. Clinical and experimental studies suggest that LABdiffer significantly in the effects and mechanisms of action. Those differences are not onlyamong different species but also among different strains within the same species. Becauseof that, the most important goal of this study was to develop a model of NAFLD (steatosisstadium) with use of high fat diet (HFD) and then to examine and to compare the effects oforal administration of two different LAB species/strains, Lactobacillus rhamnosus LA68and Lactobacillus plantarum WCFS1, on immunological and metabolic parameters, onsteatosis development status, as well as on fatty acid composition of liver and brain inC57BL/6 mice under high fat diet regime...",
publisher = "Универзитет у Београду, Фармацеутски факултет",
journal = "Универзитет у Београду",
title = "Uticaj oralne primene Lactobacillus rhamnosus LA68 i Lactobacillus plantarum WCFS1 na imunološke i metaboličke parametre miševa u uslovima eksperimentalno indukovane nealkoholne masne jetre",
url = "https://hdl.handle.net/21.15107/rcub_nardus_6949"
}

Ivanović, N.. (2016). Uticaj oralne primene Lactobacillus rhamnosus LA68 i Lactobacillus plantarum WCFS1 na imunološke i metaboličke parametre miševa u uslovima eksperimentalno indukovane nealkoholne masne jetre. in Универзитет у Београду
Универзитет у Београду, Фармацеутски факултет..
https://hdl.handle.net/21.15107/rcub_nardus_6949

Ivanović N. Uticaj oralne primene Lactobacillus rhamnosus LA68 i Lactobacillus plantarum WCFS1 na imunološke i metaboličke parametre miševa u uslovima eksperimentalno indukovane nealkoholne masne jetre. in Универзитет у Београду. 2016;.
https://hdl.handle.net/21.15107/rcub_nardus_6949 .

Ivanović, Nevena, "Uticaj oralne primene Lactobacillus rhamnosus LA68 i Lactobacillus plantarum WCFS1 na imunološke i metaboličke parametre miševa u uslovima eksperimentalno indukovane nealkoholne masne jetre" in Универзитет у Београду (2016),
https://hdl.handle.net/21.15107/rcub_nardus_6949 .

TY  - JOUR
AU  - Ivanović, Nevena
AU  - Minić, Rajna
AU  - Đuričić, Ivana
AU  - Dimitrijević, Ljiljana
AU  - Šobajić, Slađana
AU  - Živković, Irena
AU  - Đorđević, Brižita
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2399
AB  - Recent reports suggest that the metabolic activity of the enteric microbiota may influence the fatty acid composition of the host tissue. There are many studies dealing with the influence of lactobacilli on various pathological conditions, and some of the effects are strain-specific. This study was designed to test the effects of a particular Lactobacillus strain, Lactobacillus rhamnosus LA68 on fatty acid composition of the liver and the brain of C57BL/6 mice in the absence of an underlying pathological condition. Female mice were supplemented with live L. rhamnosus LA68 bacteria for the duration of 1 month. Serum biochemistry was analyzed and liver and brain fatty acid composition was assessed by gas-liquid chromatography. Significant changes in liver and brain fatty acid composition were detected. In the liver tissue we detected an increase in palmitoleic acid (p = 0.038), while in the brain compartment we found an increase in palmitic (p = 0.042), stearic (p = 0.017), arachidonic acid (p = 0.009) and docosahexaenoic acid (p = 0.004) for control versus experimental group. These results show discrete changes caused by LA68 strain consumption. Even short duration of administration of LA68 influences the fatty acid composition of the host which adds to the existing knowledge about Lactobacillus host interaction, and adds to the growing knowledge of metabolic intervention possibilities.
PB  - Informa Healthcare, London
T2  - International Journal of Food Sciences and Nutrition
T1  - Brain and liver fatty acid composition changes upon consumption of Lactobacillus rhamnosus LA68
VL  - 66
IS  - 1
SP  - 93
EP  - 97
DO  - 10.3109/09637486.2014.979313
ER  - 

@article{
author = "Ivanović, Nevena and Minić, Rajna and Đuričić, Ivana and Dimitrijević, Ljiljana and Šobajić, Slađana and Živković, Irena and Đorđević, Brižita",
year = "2015",
abstract = "Recent reports suggest that the metabolic activity of the enteric microbiota may influence the fatty acid composition of the host tissue. There are many studies dealing with the influence of lactobacilli on various pathological conditions, and some of the effects are strain-specific. This study was designed to test the effects of a particular Lactobacillus strain, Lactobacillus rhamnosus LA68 on fatty acid composition of the liver and the brain of C57BL/6 mice in the absence of an underlying pathological condition. Female mice were supplemented with live L. rhamnosus LA68 bacteria for the duration of 1 month. Serum biochemistry was analyzed and liver and brain fatty acid composition was assessed by gas-liquid chromatography. Significant changes in liver and brain fatty acid composition were detected. In the liver tissue we detected an increase in palmitoleic acid (p = 0.038), while in the brain compartment we found an increase in palmitic (p = 0.042), stearic (p = 0.017), arachidonic acid (p = 0.009) and docosahexaenoic acid (p = 0.004) for control versus experimental group. These results show discrete changes caused by LA68 strain consumption. Even short duration of administration of LA68 influences the fatty acid composition of the host which adds to the existing knowledge about Lactobacillus host interaction, and adds to the growing knowledge of metabolic intervention possibilities.",
publisher = "Informa Healthcare, London",
journal = "International Journal of Food Sciences and Nutrition",
title = "Brain and liver fatty acid composition changes upon consumption of Lactobacillus rhamnosus LA68",
volume = "66",
number = "1",
pages = "93-97",
doi = "10.3109/09637486.2014.979313"
}

Ivanović, N., Minić, R., Đuričić, I., Dimitrijević, L., Šobajić, S., Živković, I.,& Đorđević, B.. (2015). Brain and liver fatty acid composition changes upon consumption of Lactobacillus rhamnosus LA68. in International Journal of Food Sciences and Nutrition
Informa Healthcare, London., 66(1), 93-97.
https://doi.org/10.3109/09637486.2014.979313

Ivanović N, Minić R, Đuričić I, Dimitrijević L, Šobajić S, Živković I, Đorđević B. Brain and liver fatty acid composition changes upon consumption of Lactobacillus rhamnosus LA68. in International Journal of Food Sciences and Nutrition. 2015;66(1):93-97.
doi:10.3109/09637486.2014.979313 .

Ivanović, Nevena, Minić, Rajna, Đuričić, Ivana, Dimitrijević, Ljiljana, Šobajić, Slađana, Živković, Irena, Đorđević, Brižita, "Brain and liver fatty acid composition changes upon consumption of Lactobacillus rhamnosus LA68" in International Journal of Food Sciences and Nutrition, 66, no. 1 (2015):93-97,
https://doi.org/10.3109/09637486.2014.979313 . .

TY  - JOUR
AU  - Ivanović, Nevena
AU  - Minić, Rajna
AU  - Dimitrijević, Ljiljana
AU  - Radojević-Skodrić, Sanja
AU  - Živković, Irena
AU  - Đorđević, Brižita
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2390
AB  - In this study, two Lactobacillus strains (L. rhamnosus LA68 and L. plantarum WCFS1) were evaluated for their effects on high fat diet induced pathology in mice. The aim was to determine whether the administration of lactic acid bacteria had beneficial effects on ameliorating pathology. C57BL/6 mice fed a high fat diet were orally administered with the Lactobacillus strains. Both the metabolic and immunological parameters were analyzed. The administration of both of the strains had beneficial effects on mouse weight, serum cholesterol, TNF-alpha levels and liver histology. LA68 lowered the total cholesterol and HDL levels more prominently, whereas WCFS1 was more potent in lowering the TG and LDL levels. Leptin and adiponectin levels were increased in all experimental groups to different extents. The administration of L. plantarum WCFS1 led to a marked increase in leptin levels, as well as an increase in CD3+CD4+ and CD3+CD8+ cells, and a decrease of CD25+ cells, and had a lowering effect on IL-6 production and cell metabolic activity. In conclusion, active administration of both Lactobacillus strains had a positive effect on HFD-induced pathology. Although both of the tested strains had beneficial effects, oral administration of WCFS1 increased leptin levels and had a more prominent immunomodulatory effect, which should be taken into consideration in case of humane usage.
PB  - Royal Soc Chemistry, Cambridge
T2  - Food & Function
T1  - Lactobacillus rhamnosus LA68 and Lactobacillus plantarum WCFS1 differently influence metabolic and immunological parameters in high fat diet-induced hypercholesterolemia and hepatic steatosis
VL  - 6
IS  - 2
SP  - 558
EP  - 565
DO  - 10.1039/c4fo00843j
ER  - 

@article{
author = "Ivanović, Nevena and Minić, Rajna and Dimitrijević, Ljiljana and Radojević-Skodrić, Sanja and Živković, Irena and Đorđević, Brižita",
year = "2015",
abstract = "In this study, two Lactobacillus strains (L. rhamnosus LA68 and L. plantarum WCFS1) were evaluated for their effects on high fat diet induced pathology in mice. The aim was to determine whether the administration of lactic acid bacteria had beneficial effects on ameliorating pathology. C57BL/6 mice fed a high fat diet were orally administered with the Lactobacillus strains. Both the metabolic and immunological parameters were analyzed. The administration of both of the strains had beneficial effects on mouse weight, serum cholesterol, TNF-alpha levels and liver histology. LA68 lowered the total cholesterol and HDL levels more prominently, whereas WCFS1 was more potent in lowering the TG and LDL levels. Leptin and adiponectin levels were increased in all experimental groups to different extents. The administration of L. plantarum WCFS1 led to a marked increase in leptin levels, as well as an increase in CD3+CD4+ and CD3+CD8+ cells, and a decrease of CD25+ cells, and had a lowering effect on IL-6 production and cell metabolic activity. In conclusion, active administration of both Lactobacillus strains had a positive effect on HFD-induced pathology. Although both of the tested strains had beneficial effects, oral administration of WCFS1 increased leptin levels and had a more prominent immunomodulatory effect, which should be taken into consideration in case of humane usage.",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Food & Function",
title = "Lactobacillus rhamnosus LA68 and Lactobacillus plantarum WCFS1 differently influence metabolic and immunological parameters in high fat diet-induced hypercholesterolemia and hepatic steatosis",
volume = "6",
number = "2",
pages = "558-565",
doi = "10.1039/c4fo00843j"
}

Ivanović, N., Minić, R., Dimitrijević, L., Radojević-Skodrić, S., Živković, I.,& Đorđević, B.. (2015). Lactobacillus rhamnosus LA68 and Lactobacillus plantarum WCFS1 differently influence metabolic and immunological parameters in high fat diet-induced hypercholesterolemia and hepatic steatosis. in Food & Function
Royal Soc Chemistry, Cambridge., 6(2), 558-565.
https://doi.org/10.1039/c4fo00843j

Ivanović N, Minić R, Dimitrijević L, Radojević-Skodrić S, Živković I, Đorđević B. Lactobacillus rhamnosus LA68 and Lactobacillus plantarum WCFS1 differently influence metabolic and immunological parameters in high fat diet-induced hypercholesterolemia and hepatic steatosis. in Food & Function. 2015;6(2):558-565.
doi:10.1039/c4fo00843j .

Ivanović, Nevena, Minić, Rajna, Dimitrijević, Ljiljana, Radojević-Skodrić, Sanja, Živković, Irena, Đorđević, Brižita, "Lactobacillus rhamnosus LA68 and Lactobacillus plantarum WCFS1 differently influence metabolic and immunological parameters in high fat diet-induced hypercholesterolemia and hepatic steatosis" in Food & Function, 6, no. 2 (2015):558-565,
https://doi.org/10.1039/c4fo00843j . .

TY  - JOUR
AU  - Dimitrijević, Rajna
AU  - Ivanović, Nevena
AU  - Mathiesen, Geir
AU  - Petrusić, Vladimir
AU  - Živković, Irena
AU  - Đorđević, Brižita
AU  - Dimitrijević, Ljiljana
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2165
AB  - Probiotic bacteria have been used in human nutrition for centuries and are now attracting more attention. In order to examine the immunological aspects of probiotic consumption, Lactobacillus rhamnosus LA68 was orally administrated using gavage to healthy C57BL/6 mice. After one month splenocytes were isolated, and analysed by flow cytometry. The magnitude of splenocyte proliferation upon stimulation with lipopolysaccharide and peptidoglycan and cytokine levels (IFN-gamma, IL-6, IL-10 and IL-17) was assessed. Cytokine levels in the serum were also analysed. Oral application of strain LA68 leads to a significant decrease of CD3+, CD25+ and CD19+ cells, and an increase of CD11b+ and CD16/CD32+ positive cell populations in the mouse spleen. Increased sensitivity to stimulation through proliferation and IL-6 secretion was detected. Increased serum IFN-gamma and decreased IL-10 levels were found. Our results show increased responsiveness of splenocytes, activation of the Th1 type of immune response, and a shift of leucocyte populations towards monocyte/granulocyte populations.
PB  - Cambridge Univ Press, New York
T2  - Journal of Dairy Research
T1  - Effects of Lactobacillus rhamnosus LA68 on the immune system of C57BL/6 mice upon oral administration
VL  - 81
IS  - 2
SP  - 202
EP  - 207
DO  - 10.1017/S0022029914000028
ER  - 

@article{
author = "Dimitrijević, Rajna and Ivanović, Nevena and Mathiesen, Geir and Petrusić, Vladimir and Živković, Irena and Đorđević, Brižita and Dimitrijević, Ljiljana",
year = "2014",
abstract = "Probiotic bacteria have been used in human nutrition for centuries and are now attracting more attention. In order to examine the immunological aspects of probiotic consumption, Lactobacillus rhamnosus LA68 was orally administrated using gavage to healthy C57BL/6 mice. After one month splenocytes were isolated, and analysed by flow cytometry. The magnitude of splenocyte proliferation upon stimulation with lipopolysaccharide and peptidoglycan and cytokine levels (IFN-gamma, IL-6, IL-10 and IL-17) was assessed. Cytokine levels in the serum were also analysed. Oral application of strain LA68 leads to a significant decrease of CD3+, CD25+ and CD19+ cells, and an increase of CD11b+ and CD16/CD32+ positive cell populations in the mouse spleen. Increased sensitivity to stimulation through proliferation and IL-6 secretion was detected. Increased serum IFN-gamma and decreased IL-10 levels were found. Our results show increased responsiveness of splenocytes, activation of the Th1 type of immune response, and a shift of leucocyte populations towards monocyte/granulocyte populations.",
publisher = "Cambridge Univ Press, New York",
journal = "Journal of Dairy Research",
title = "Effects of Lactobacillus rhamnosus LA68 on the immune system of C57BL/6 mice upon oral administration",
volume = "81",
number = "2",
pages = "202-207",
doi = "10.1017/S0022029914000028"
}

Dimitrijević, R., Ivanović, N., Mathiesen, G., Petrusić, V., Živković, I., Đorđević, B.,& Dimitrijević, L.. (2014). Effects of Lactobacillus rhamnosus LA68 on the immune system of C57BL/6 mice upon oral administration. in Journal of Dairy Research
Cambridge Univ Press, New York., 81(2), 202-207.
https://doi.org/10.1017/S0022029914000028

Dimitrijević R, Ivanović N, Mathiesen G, Petrusić V, Živković I, Đorđević B, Dimitrijević L. Effects of Lactobacillus rhamnosus LA68 on the immune system of C57BL/6 mice upon oral administration. in Journal of Dairy Research. 2014;81(2):202-207.
doi:10.1017/S0022029914000028 .

Dimitrijević, Rajna, Ivanović, Nevena, Mathiesen, Geir, Petrusić, Vladimir, Živković, Irena, Đorđević, Brižita, Dimitrijević, Ljiljana, "Effects of Lactobacillus rhamnosus LA68 on the immune system of C57BL/6 mice upon oral administration" in Journal of Dairy Research, 81, no. 2 (2014):202-207,
https://doi.org/10.1017/S0022029914000028 . .