TY  - JOUR
AU  - Grozdanić, Nađa
AU  - Đuričić, Ivana
AU  - Kosanić, Marijana
AU  - Zdunić, Gordana
AU  - Šavikin, Katarina
AU  - Etahiri, Samira
AU  - Assobhei, Omar
AU  - Benba, Jamila
AU  - Petović, Slavica
AU  - Matić, Ivana Z.
AU  - Stanojković, Tatjana P.
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3605
AB  - Purpose: Sea macroalgae are an important source of biologically highly valuable compounds. The main aim of this study was to investigate the in vitro anticancer properties and chemical composition of the dichloromethane-methanol extract and three fractions of the Fucus spiralis from coastline of Morocco. Methods: Fractions were made from dichloromethane: methanol (1:1) extract of Fucus spiralis: petroleum-ether, ethyl-acetate and n-butanol. Extract and fractions were screened for in vitro cytotoxicity by MTT assay against human cervical adenocarcinoma (HeLa), colorectal adenocarcinoma (LS-174T), lung carcinoma (A549), and normal human lung fibroblasts (MRC-5). Cell cycle distribution of the HeLa cells was evaluated using flow cytometry. Acridine orange (AO)-ethidium bromide (EB) staining was used to assess morphological changes of HeLa cells under fluorescence microscope. Anti-migration and anti-angiogenic properties were investigated using scratch and tube formation assays against human endothelium-derived permanent EA.hy926 cell line. Antidiabetic activity was tested using anti-α-glucosidase assay. Antimicrobial effect was tested using micro- dilution method. Results: Petroleum-ether fraction оf Fucus spiralis rich in fatty acids exerted the highest cytotoxicity against HeLa cells. Ethyl-acetate and petroleum-ether fractions induced the highest accumulation of the HeLa cells in sub-G1 and G2/M phases. Extract and fractions showed proapoptotic effect on HeLa cells under fluorescent microscope. They exhibited antimigratory and antiangiogenic effects in vitro. IC50 value for α-glucosidase inhibitory activity was much stronger than standard acarbose. n-Butanol fraction exerted the highest antibacterial and antifungal activity. Conclusions: The investigation of various biological activities of the extract and fractions obtained from Fucus spiralis may suggest a promising anticancer and pharmacological potential of this edible macroalga.
PB  - Zerbinis Publications
T2  - Journal of B.U.ON.
T1  - Fucus spiralis extract and fractions: Anticancer and pharmacological potentials
VL  - 25
IS  - 2
SP  - 1219
EP  - 1229
UR  - https://hdl.handle.net/21.15107/rcub_farfar_3605
ER  - 

@article{
author = "Grozdanić, Nađa and Đuričić, Ivana and Kosanić, Marijana and Zdunić, Gordana and Šavikin, Katarina and Etahiri, Samira and Assobhei, Omar and Benba, Jamila and Petović, Slavica and Matić, Ivana Z. and Stanojković, Tatjana P.",
year = "2020",
abstract = "Purpose: Sea macroalgae are an important source of biologically highly valuable compounds. The main aim of this study was to investigate the in vitro anticancer properties and chemical composition of the dichloromethane-methanol extract and three fractions of the Fucus spiralis from coastline of Morocco. Methods: Fractions were made from dichloromethane: methanol (1:1) extract of Fucus spiralis: petroleum-ether, ethyl-acetate and n-butanol. Extract and fractions were screened for in vitro cytotoxicity by MTT assay against human cervical adenocarcinoma (HeLa), colorectal adenocarcinoma (LS-174T), lung carcinoma (A549), and normal human lung fibroblasts (MRC-5). Cell cycle distribution of the HeLa cells was evaluated using flow cytometry. Acridine orange (AO)-ethidium bromide (EB) staining was used to assess morphological changes of HeLa cells under fluorescence microscope. Anti-migration and anti-angiogenic properties were investigated using scratch and tube formation assays against human endothelium-derived permanent EA.hy926 cell line. Antidiabetic activity was tested using anti-α-glucosidase assay. Antimicrobial effect was tested using micro- dilution method. Results: Petroleum-ether fraction оf Fucus spiralis rich in fatty acids exerted the highest cytotoxicity against HeLa cells. Ethyl-acetate and petroleum-ether fractions induced the highest accumulation of the HeLa cells in sub-G1 and G2/M phases. Extract and fractions showed proapoptotic effect on HeLa cells under fluorescent microscope. They exhibited antimigratory and antiangiogenic effects in vitro. IC50 value for α-glucosidase inhibitory activity was much stronger than standard acarbose. n-Butanol fraction exerted the highest antibacterial and antifungal activity. Conclusions: The investigation of various biological activities of the extract and fractions obtained from Fucus spiralis may suggest a promising anticancer and pharmacological potential of this edible macroalga.",
publisher = "Zerbinis Publications",
journal = "Journal of B.U.ON.",
title = "Fucus spiralis extract and fractions: Anticancer and pharmacological potentials",
volume = "25",
number = "2",
pages = "1219-1229",
url = "https://hdl.handle.net/21.15107/rcub_farfar_3605"
}

Grozdanić, N., Đuričić, I., Kosanić, M., Zdunić, G., Šavikin, K., Etahiri, S., Assobhei, O., Benba, J., Petović, S., Matić, I. Z.,& Stanojković, T. P.. (2020). Fucus spiralis extract and fractions: Anticancer and pharmacological potentials. in Journal of B.U.ON.
Zerbinis Publications., 25(2), 1219-1229.
https://hdl.handle.net/21.15107/rcub_farfar_3605

Grozdanić N, Đuričić I, Kosanić M, Zdunić G, Šavikin K, Etahiri S, Assobhei O, Benba J, Petović S, Matić IZ, Stanojković TP. Fucus spiralis extract and fractions: Anticancer and pharmacological potentials. in Journal of B.U.ON.. 2020;25(2):1219-1229.
https://hdl.handle.net/21.15107/rcub_farfar_3605 .

Grozdanić, Nađa, Đuričić, Ivana, Kosanić, Marijana, Zdunić, Gordana, Šavikin, Katarina, Etahiri, Samira, Assobhei, Omar, Benba, Jamila, Petović, Slavica, Matić, Ivana Z., Stanojković, Tatjana P., "Fucus spiralis extract and fractions: Anticancer and pharmacological potentials" in Journal of B.U.ON., 25, no. 2 (2020):1219-1229,
https://hdl.handle.net/21.15107/rcub_farfar_3605 .

TY  - JOUR
AU  - Milović, Sanja
AU  - Stanković, Ivan
AU  - Nikolić, Dejan
AU  - Radović, Jelena
AU  - Kolundžić, Marina
AU  - Nikolić, Vesna
AU  - Stanojković, Tatjana
AU  - Petović, Slavica
AU  - Kundaković-Vasović, Tatjana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3468
AB  - Three seaweeds (Halimeda tuna, Codium bursa and Cystoseira barbata) and one seagrass (Cymodocea nodosa) were collected from the Coast of Montenegro, Gulf of Boka Kotorska and their chemical analysis was performed. In seagrass C. nodosa, three phenolic compounds were identified (diosmetin 7-sulfate, caftaric and coutaric acid). The content of β-glucan, fatty acids, sterols and micro- and macro-elements were investigated among all samples. The highest content of β-glucan was detected in C. nodosa seagrass (13.04±0.42 g/100 g). The highest polyunsaturated fatty acids (PUFAs) level was reported in C. barbata, the brown alga (7.157 mg/g), which also had the significant sterol content (fucosterol, 21.76±0.1 μg/g). Green algae, C. bursa and H. tuna, showed the highest level of sterols (β-sitosterol, 95.21±0.16 μg/g and 73.90±0.08 μg/g, respectively). H. tuna had the highest content of calcium (Ca) in amount of 55125 μg/g. In C. bursa, C. barbata and C. nodosa, the Na/K ratio was low (0.43, 0.46 and 0.69, respectively).
PB  - Wiley
T2  - Chemistry and Biodiversity
T1  - Chemical Analysis of Selected Seaweeds and Seagrass from the Adriatic Coast of Montenegro
VL  - 16
IS  - 10
SP  - 1
EP  - 11
DO  - 10.1002/cbdv.201900327
ER  - 

@article{
author = "Milović, Sanja and Stanković, Ivan and Nikolić, Dejan and Radović, Jelena and Kolundžić, Marina and Nikolić, Vesna and Stanojković, Tatjana and Petović, Slavica and Kundaković-Vasović, Tatjana",
year = "2019",
abstract = "Three seaweeds (Halimeda tuna, Codium bursa and Cystoseira barbata) and one seagrass (Cymodocea nodosa) were collected from the Coast of Montenegro, Gulf of Boka Kotorska and their chemical analysis was performed. In seagrass C. nodosa, three phenolic compounds were identified (diosmetin 7-sulfate, caftaric and coutaric acid). The content of β-glucan, fatty acids, sterols and micro- and macro-elements were investigated among all samples. The highest content of β-glucan was detected in C. nodosa seagrass (13.04±0.42 g/100 g). The highest polyunsaturated fatty acids (PUFAs) level was reported in C. barbata, the brown alga (7.157 mg/g), which also had the significant sterol content (fucosterol, 21.76±0.1 μg/g). Green algae, C. bursa and H. tuna, showed the highest level of sterols (β-sitosterol, 95.21±0.16 μg/g and 73.90±0.08 μg/g, respectively). H. tuna had the highest content of calcium (Ca) in amount of 55125 μg/g. In C. bursa, C. barbata and C. nodosa, the Na/K ratio was low (0.43, 0.46 and 0.69, respectively).",
publisher = "Wiley",
journal = "Chemistry and Biodiversity",
title = "Chemical Analysis of Selected Seaweeds and Seagrass from the Adriatic Coast of Montenegro",
volume = "16",
number = "10",
pages = "1-11",
doi = "10.1002/cbdv.201900327"
}

Milović, S., Stanković, I., Nikolić, D., Radović, J., Kolundžić, M., Nikolić, V., Stanojković, T., Petović, S.,& Kundaković-Vasović, T.. (2019). Chemical Analysis of Selected Seaweeds and Seagrass from the Adriatic Coast of Montenegro. in Chemistry and Biodiversity
Wiley., 16(10), 1-11.
https://doi.org/10.1002/cbdv.201900327

Milović S, Stanković I, Nikolić D, Radović J, Kolundžić M, Nikolić V, Stanojković T, Petović S, Kundaković-Vasović T. Chemical Analysis of Selected Seaweeds and Seagrass from the Adriatic Coast of Montenegro. in Chemistry and Biodiversity. 2019;16(10):1-11.
doi:10.1002/cbdv.201900327 .

Milović, Sanja, Stanković, Ivan, Nikolić, Dejan, Radović, Jelena, Kolundžić, Marina, Nikolić, Vesna, Stanojković, Tatjana, Petović, Slavica, Kundaković-Vasović, Tatjana, "Chemical Analysis of Selected Seaweeds and Seagrass from the Adriatic Coast of Montenegro" in Chemistry and Biodiversity, 16, no. 10 (2019):1-11,
https://doi.org/10.1002/cbdv.201900327 . .

TY  - JOUR
AU  - Milović, Sanja
AU  - Stanković, Ivan
AU  - Nikolić, Dejan
AU  - Radović, Jelena
AU  - Kolundžić, Marina
AU  - Nikolić, Vesna
AU  - Stanojković, Tatjana
AU  - Petović, Slavica
AU  - Kundaković-Vasović, Tatjana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3463
AB  - Three seaweeds (Halimeda tuna, Codium bursa and Cystoseira barbata) and one seagrass (Cymodocea nodosa) were collected from the Coast of Montenegro, Gulf of Boka Kotorska and their chemical analysis was performed. In seagrass C. nodosa, three phenolic compounds were identified (diosmetin 7-sulfate, caftaric and coutaric acid). The content of β-glucan, fatty acids, sterols and micro- and macro-elements were investigated among all samples. The highest content of β-glucan was detected in C. nodosa seagrass (13.04±0.42 g/100 g). The highest polyunsaturated fatty acids (PUFAs) level was reported in C. barbata, the brown alga (7.157 mg/g), which also had the significant sterol content (fucosterol, 21.76±0.1 μg/g). Green algae, C. bursa and H. tuna, showed the highest level of sterols (β-sitosterol, 95.21±0.16 μg/g and 73.90±0.08 μg/g, respectively). H. tuna had the highest content of calcium (Ca) in amount of 55125 μg/g. In C. bursa, C. barbata and C. nodosa, the Na/K ratio was low (0.43, 0.46 and 0.69, respectively).
PB  - Wiley
T2  - Chemistry and Biodiversity
T1  - Chemical Analysis of Selected Seaweeds and Seagrass from the Adriatic Coast of Montenegro
VL  - 16
IS  - 10
SP  - 1
EP  - 11
DO  - 10.1002/cbdv.201900327
ER  - 

@article{
author = "Milović, Sanja and Stanković, Ivan and Nikolić, Dejan and Radović, Jelena and Kolundžić, Marina and Nikolić, Vesna and Stanojković, Tatjana and Petović, Slavica and Kundaković-Vasović, Tatjana",
year = "2019",
abstract = "Three seaweeds (Halimeda tuna, Codium bursa and Cystoseira barbata) and one seagrass (Cymodocea nodosa) were collected from the Coast of Montenegro, Gulf of Boka Kotorska and their chemical analysis was performed. In seagrass C. nodosa, three phenolic compounds were identified (diosmetin 7-sulfate, caftaric and coutaric acid). The content of β-glucan, fatty acids, sterols and micro- and macro-elements were investigated among all samples. The highest content of β-glucan was detected in C. nodosa seagrass (13.04±0.42 g/100 g). The highest polyunsaturated fatty acids (PUFAs) level was reported in C. barbata, the brown alga (7.157 mg/g), which also had the significant sterol content (fucosterol, 21.76±0.1 μg/g). Green algae, C. bursa and H. tuna, showed the highest level of sterols (β-sitosterol, 95.21±0.16 μg/g and 73.90±0.08 μg/g, respectively). H. tuna had the highest content of calcium (Ca) in amount of 55125 μg/g. In C. bursa, C. barbata and C. nodosa, the Na/K ratio was low (0.43, 0.46 and 0.69, respectively).",
publisher = "Wiley",
journal = "Chemistry and Biodiversity",
title = "Chemical Analysis of Selected Seaweeds and Seagrass from the Adriatic Coast of Montenegro",
volume = "16",
number = "10",
pages = "1-11",
doi = "10.1002/cbdv.201900327"
}

Milović, S., Stanković, I., Nikolić, D., Radović, J., Kolundžić, M., Nikolić, V., Stanojković, T., Petović, S.,& Kundaković-Vasović, T.. (2019). Chemical Analysis of Selected Seaweeds and Seagrass from the Adriatic Coast of Montenegro. in Chemistry and Biodiversity
Wiley., 16(10), 1-11.
https://doi.org/10.1002/cbdv.201900327

Milović S, Stanković I, Nikolić D, Radović J, Kolundžić M, Nikolić V, Stanojković T, Petović S, Kundaković-Vasović T. Chemical Analysis of Selected Seaweeds and Seagrass from the Adriatic Coast of Montenegro. in Chemistry and Biodiversity. 2019;16(10):1-11.
doi:10.1002/cbdv.201900327 .

Milović, Sanja, Stanković, Ivan, Nikolić, Dejan, Radović, Jelena, Kolundžić, Marina, Nikolić, Vesna, Stanojković, Tatjana, Petović, Slavica, Kundaković-Vasović, Tatjana, "Chemical Analysis of Selected Seaweeds and Seagrass from the Adriatic Coast of Montenegro" in Chemistry and Biodiversity, 16, no. 10 (2019):1-11,
https://doi.org/10.1002/cbdv.201900327 . .

TY  - CONF
AU  - Milutinović, Violeta
AU  - Matić, Ivana
AU  - Grozdanić-Stanisavljević, Nađa
AU  - Stanojković, Tatjana
AU  - Soković, Marina
AU  - Ćirić, Ana
AU  - Niketić, Marjan
AU  - Petrović, Silvana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5109
AB  - Cytotoxic and antimicrobial activities were investigated for MeOH extracts of flowering aerial parts of Hieracium calophyllum R. Uechtr. (CAL), H. coloriscapum Rohlena & Zahn (COL), H. pseudoschenkii (Rohlena & Zahn) Niketić (PSE), H. valdepilosum Vill. s.l. (VAL) and H. glabratum Willd. (GLA), their two isolated sesquiterpene lactones 8-epiixerisamine A and crepiside E, as well as for CH2Cl2 extract of flowering aerial parts of H. scheppigianum Freyn (SCH). Crepiside E (IC50 = 46.00 μg/mL), COL (IC50 = 152.28 μg/mL) and SCH (IC50 = 148.12 μg/mL) showed 
the highest cytotoxic activity against HeLa cells in MTT test. The cytotoxic activity against LS174, A549, as well as against normal MRC-5 cells was significantly weaker. In microdilution test, crepiside E and 8-epiixerisamine A exhibited noteworthy antifungal effect against Aspergillus ochraceus, Penicillium funiculosum, Candida albicans and C. krusei (MIC = 0.15 - 0.4 mg/mL, MFC = 0.3 - 0.8 mg/mL). Among the extracts, the best antibacterial activity was shown by SCH and CAL against Pseudomonas aeruginosa (MIC = 1.68 and 2.52 mg/mL, MBC = 3.36 and 5.04 mg/mL), and the highest antifungal activity by SCH and VAL against C. albicans (MIC = 2.48 and 2.52 mg/mL, MFC = 4.96 and 5.04 mg/mL).
PB  - Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš
PB  - Institute for Nature Conservation of Serbia
C3  - 13th Symposium on the Flora of  Southeastern Serbia  and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts
T1  - Cytotoxic and antimicrobial activity of selected Hieracium L. extracts and isolated sesquiterpene lactones
SP  - 162
EP  - 163
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5109
ER  - 

@conference{
author = "Milutinović, Violeta and Matić, Ivana and Grozdanić-Stanisavljević, Nađa and Stanojković, Tatjana and Soković, Marina and Ćirić, Ana and Niketić, Marjan and Petrović, Silvana",
year = "2019",
abstract = "Cytotoxic and antimicrobial activities were investigated for MeOH extracts of flowering aerial parts of Hieracium calophyllum R. Uechtr. (CAL), H. coloriscapum Rohlena & Zahn (COL), H. pseudoschenkii (Rohlena & Zahn) Niketić (PSE), H. valdepilosum Vill. s.l. (VAL) and H. glabratum Willd. (GLA), their two isolated sesquiterpene lactones 8-epiixerisamine A and crepiside E, as well as for CH2Cl2 extract of flowering aerial parts of H. scheppigianum Freyn (SCH). Crepiside E (IC50 = 46.00 μg/mL), COL (IC50 = 152.28 μg/mL) and SCH (IC50 = 148.12 μg/mL) showed 
the highest cytotoxic activity against HeLa cells in MTT test. The cytotoxic activity against LS174, A549, as well as against normal MRC-5 cells was significantly weaker. In microdilution test, crepiside E and 8-epiixerisamine A exhibited noteworthy antifungal effect against Aspergillus ochraceus, Penicillium funiculosum, Candida albicans and C. krusei (MIC = 0.15 - 0.4 mg/mL, MFC = 0.3 - 0.8 mg/mL). Among the extracts, the best antibacterial activity was shown by SCH and CAL against Pseudomonas aeruginosa (MIC = 1.68 and 2.52 mg/mL, MBC = 3.36 and 5.04 mg/mL), and the highest antifungal activity by SCH and VAL against C. albicans (MIC = 2.48 and 2.52 mg/mL, MFC = 4.96 and 5.04 mg/mL).",
publisher = "Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, Institute for Nature Conservation of Serbia",
journal = "13th Symposium on the Flora of  Southeastern Serbia  and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts",
title = "Cytotoxic and antimicrobial activity of selected Hieracium L. extracts and isolated sesquiterpene lactones",
pages = "162-163",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5109"
}

Milutinović, V., Matić, I., Grozdanić-Stanisavljević, N., Stanojković, T., Soković, M., Ćirić, A., Niketić, M.,& Petrović, S.. (2019). Cytotoxic and antimicrobial activity of selected Hieracium L. extracts and isolated sesquiterpene lactones. in 13th Symposium on the Flora of  Southeastern Serbia  and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts
Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš., 162-163.
https://hdl.handle.net/21.15107/rcub_farfar_5109

Milutinović V, Matić I, Grozdanić-Stanisavljević N, Stanojković T, Soković M, Ćirić A, Niketić M, Petrović S. Cytotoxic and antimicrobial activity of selected Hieracium L. extracts and isolated sesquiterpene lactones. in 13th Symposium on the Flora of  Southeastern Serbia  and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts. 2019;:162-163.
https://hdl.handle.net/21.15107/rcub_farfar_5109 .

Milutinović, Violeta, Matić, Ivana, Grozdanić-Stanisavljević, Nađa, Stanojković, Tatjana, Soković, Marina, Ćirić, Ana, Niketić, Marjan, Petrović, Silvana, "Cytotoxic and antimicrobial activity of selected Hieracium L. extracts and isolated sesquiterpene lactones" in 13th Symposium on the Flora of  Southeastern Serbia  and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts (2019):162-163,
https://hdl.handle.net/21.15107/rcub_farfar_5109 .

TY  - JOUR
AU  - Antonijević Nikolić, Mirjana
AU  - Dražić, Branka
AU  - Antić-Stanković, Jelena
AU  - Tanasković, Slađana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4379
AB  - New carboxylate complexes of the tetraazamacrocyclic ligand
N,N',N'',N'''-tetrakis(2-pyridilmethyl)-1,4,8,11-tetraazacyclotetradecane (tpmc)
with Ni(II) and Zn(II) as central ions were prepared. In mixed-ligand com-
plexes (endo,endo)-bicyclo[2.2.1]hept-5-ene-2,3-dicarboxylate dianion (C9H8O42-
)
is also coordinated to metal ions. The complexes were characterized by ele-
mental analysis (C, H, N), FTIR and UV–Vis spectroscopy, molar conductivity
determination and magnetic susceptibility measurement at room temperature.
The analytical data of the complexes show the formation of binuclear
[Ni2(C9H8O4)tpmc](ClO4)2·4H2O and tetranuclear [Zn4(C9H8O4)(tpmc)2](ClO4)6·
·CH3CN·KClO 4·4H2O complexes. In tetranuclear Zn(II) complex bicyclic
dicarboxylate ligand is most likely to be bridge coordinated, and in binuclear
Ni(II) complex it is coordinated in a combined bridged manner with chelate
rings formation. In both complexes macrocyclic ligand was exo coordinated,
out of cyclam ring and adopts a boat conformation. The Zn(II) complex is one
of the rare tetranuclear Zn(II)-tpmc complexes with carboxylate ion bridging
two Zn2tpmc units. The complexes were tested for antibacterial activity against
Gram-positive bacteria Staphylococcus aureus (ATCC 25923) and Bacillus
subtilis (ATCC 6633), Gram-negative bacterium Escherichia coli (ATCC
25922), and yeast Candida albicans (ATCC 10231), and were screened for
antiproliferative activity against human cervix adenocarcinoma (HeLa) and
human myelogenous leukemia (K562) cell lines.
AB  - Синтетисани су нови карбоксилато комплекси са тетраазамакроцикличним ли-
гандом N,N',N'',N'''-тетракис(2-пиридилметил)-1,4,8,11-тетраазациклотетрадеканом
(tpmc) и Ni(II) и Zn(II) као централним јонима. У мешовитолигандним комплексима
(endo,endo)-бицикло-[2.2.1]-хепт-5-ен-2,3-дикарбоксилато дианјон (C9H8O4
2-
) је такође
везан за метални јон. Комплекси су окарактерисани елементалном анализом (C, H, N),
FTIR и UV/Vis спектроскопијом, као и мерењем моларне проводљивости и магнетне
сусцептибилности на собној температури. На основу добијених резултата претпос-
тављена је бинуклеарна структура [Ni2(C9H8O4)tpmc](ClO4) 2·4H2O комплекса, односно,
тетрануклеарна [Zn4(C9H8O4 )(tpmc) 2](ClO4)6·CH3CN·KClO4 ·4H2O комплекса. У тетра-
нуклеарном Zn(II) комплексу, бициклични дикарбоксилато лиганд је највероватније
мостно везан, док је у бинуклеарном Ni(II) комплексу мешовити мостно-хелатни начин
координације. Други начини мостног везивања дикарбоксилата не могу бити у потпуности искључени. У оба комплекса макроциклични лиганд је егзо координован, изван
цикламовог прстена и заузима конформацију лађе. Добијени Zn(II) кoмплекс је један од
ретких тетрануклеарних Zn(II)–tpmc комплекса са карбоксилато лигандом у мосту
између две Zn2tpmc јединице. Добијени комплекси су тестирани на антибактеријску
активност према Грам-позитивним бактеријама Staphylococcus aureus (ATCC 25923) и
Bacillus subtilis (ATCC 6633), Грам-негативној бактерији Escherichia coli (ATCC 25922) и
квасцу Candida albicans (ATCC 10231). Испитивана је њихова антипролиферативна
активност на хуманим малигним ћелијским линијама: цервикалног аденокарцинома
(HeLa) и мијелогене леукемије (K562).
PB  - Serbian Chemical Society
T2  - Journal of the Serbian Chemical Society
T1  - New mixed-ligand Ni(II) and Zn(II) macrocyclic complexes with bridged bicyclo-[2,2,1]-hept-5-en-endo-2,3-cis-dicarboxylate: synthesis, characterization, antimicrobial and cytotoxic activity
T1  - Nови мешовитолигандни макроциклични комплекси Ni(II) и Zn(II) са мостовним (endo,endo)-бицикло-[2.2.1]-хепт-5-ен-2,3-дикарбоксилатом: синтеза, карактеризација, антимикробна и цитотоксична активност
VL  - 84
IS  - 9
SP  - 961
EP  - 973
DO  - https://doi.org/10.2298/JSC181216028A
ER  - 

@article{
author = "Antonijević Nikolić, Mirjana and Dražić, Branka and Antić-Stanković, Jelena and Tanasković, Slađana",
year = "2019",
abstract = "New carboxylate complexes of the tetraazamacrocyclic ligand
N,N',N'',N'''-tetrakis(2-pyridilmethyl)-1,4,8,11-tetraazacyclotetradecane (tpmc)
with Ni(II) and Zn(II) as central ions were prepared. In mixed-ligand com-
plexes (endo,endo)-bicyclo[2.2.1]hept-5-ene-2,3-dicarboxylate dianion (C9H8O42-
)
is also coordinated to metal ions. The complexes were characterized by ele-
mental analysis (C, H, N), FTIR and UV–Vis spectroscopy, molar conductivity
determination and magnetic susceptibility measurement at room temperature.
The analytical data of the complexes show the formation of binuclear
[Ni2(C9H8O4)tpmc](ClO4)2·4H2O and tetranuclear [Zn4(C9H8O4)(tpmc)2](ClO4)6·
·CH3CN·KClO 4·4H2O complexes. In tetranuclear Zn(II) complex bicyclic
dicarboxylate ligand is most likely to be bridge coordinated, and in binuclear
Ni(II) complex it is coordinated in a combined bridged manner with chelate
rings formation. In both complexes macrocyclic ligand was exo coordinated,
out of cyclam ring and adopts a boat conformation. The Zn(II) complex is one
of the rare tetranuclear Zn(II)-tpmc complexes with carboxylate ion bridging
two Zn2tpmc units. The complexes were tested for antibacterial activity against
Gram-positive bacteria Staphylococcus aureus (ATCC 25923) and Bacillus
subtilis (ATCC 6633), Gram-negative bacterium Escherichia coli (ATCC
25922), and yeast Candida albicans (ATCC 10231), and were screened for
antiproliferative activity against human cervix adenocarcinoma (HeLa) and
human myelogenous leukemia (K562) cell lines., Синтетисани су нови карбоксилато комплекси са тетраазамакроцикличним ли-
гандом N,N',N'',N'''-тетракис(2-пиридилметил)-1,4,8,11-тетраазациклотетрадеканом
(tpmc) и Ni(II) и Zn(II) као централним јонима. У мешовитолигандним комплексима
(endo,endo)-бицикло-[2.2.1]-хепт-5-ен-2,3-дикарбоксилато дианјон (C9H8O4
2-
) је такође
везан за метални јон. Комплекси су окарактерисани елементалном анализом (C, H, N),
FTIR и UV/Vis спектроскопијом, као и мерењем моларне проводљивости и магнетне
сусцептибилности на собној температури. На основу добијених резултата претпос-
тављена је бинуклеарна структура [Ni2(C9H8O4)tpmc](ClO4) 2·4H2O комплекса, односно,
тетрануклеарна [Zn4(C9H8O4 )(tpmc) 2](ClO4)6·CH3CN·KClO4 ·4H2O комплекса. У тетра-
нуклеарном Zn(II) комплексу, бициклични дикарбоксилато лиганд је највероватније
мостно везан, док је у бинуклеарном Ni(II) комплексу мешовити мостно-хелатни начин
координације. Други начини мостног везивања дикарбоксилата не могу бити у потпуности искључени. У оба комплекса макроциклични лиганд је егзо координован, изван
цикламовог прстена и заузима конформацију лађе. Добијени Zn(II) кoмплекс је један од
ретких тетрануклеарних Zn(II)–tpmc комплекса са карбоксилато лигандом у мосту
између две Zn2tpmc јединице. Добијени комплекси су тестирани на антибактеријску
активност према Грам-позитивним бактеријама Staphylococcus aureus (ATCC 25923) и
Bacillus subtilis (ATCC 6633), Грам-негативној бактерији Escherichia coli (ATCC 25922) и
квасцу Candida albicans (ATCC 10231). Испитивана је њихова антипролиферативна
активност на хуманим малигним ћелијским линијама: цервикалног аденокарцинома
(HeLa) и мијелогене леукемије (K562).",
publisher = "Serbian Chemical Society",
journal = "Journal of the Serbian Chemical Society",
title = "New mixed-ligand Ni(II) and Zn(II) macrocyclic complexes with bridged bicyclo-[2,2,1]-hept-5-en-endo-2,3-cis-dicarboxylate: synthesis, characterization, antimicrobial and cytotoxic activity, Nови мешовитолигандни макроциклични комплекси Ni(II) и Zn(II) са мостовним (endo,endo)-бицикло-[2.2.1]-хепт-5-ен-2,3-дикарбоксилатом: синтеза, карактеризација, антимикробна и цитотоксична активност",
volume = "84",
number = "9",
pages = "961-973",
doi = "https://doi.org/10.2298/JSC181216028A"
}

Antonijević Nikolić, M., Dražić, B., Antić-Stanković, J.,& Tanasković, S.. (2019). New mixed-ligand Ni(II) and Zn(II) macrocyclic complexes with bridged bicyclo-[2,2,1]-hept-5-en-endo-2,3-cis-dicarboxylate: synthesis, characterization, antimicrobial and cytotoxic activity. in Journal of the Serbian Chemical Society
Serbian Chemical Society., 84(9), 961-973.
https://doi.org/https://doi.org/10.2298/JSC181216028A

Antonijević Nikolić M, Dražić B, Antić-Stanković J, Tanasković S. New mixed-ligand Ni(II) and Zn(II) macrocyclic complexes with bridged bicyclo-[2,2,1]-hept-5-en-endo-2,3-cis-dicarboxylate: synthesis, characterization, antimicrobial and cytotoxic activity. in Journal of the Serbian Chemical Society. 2019;84(9):961-973.
doi:https://doi.org/10.2298/JSC181216028A .

Antonijević Nikolić, Mirjana, Dražić, Branka, Antić-Stanković, Jelena, Tanasković, Slađana, "New mixed-ligand Ni(II) and Zn(II) macrocyclic complexes with bridged bicyclo-[2,2,1]-hept-5-en-endo-2,3-cis-dicarboxylate: synthesis, characterization, antimicrobial and cytotoxic activity" in Journal of the Serbian Chemical Society, 84, no. 9 (2019):961-973,
https://doi.org/https://doi.org/10.2298/JSC181216028A . .

TY  - GEN
AU  - Ušjak, Ljuboš
AU  - Drobac, Milica
AU  - Soković, Marina
AU  - Stanojković, Tatjana
AU  - Niketić, Marjan
AU  - Petrović, Silvana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3482
AB  - Uvod: Predmet istraživanja je devet taksona roda Heracleum (Apiaceae): sedam iz grupe 
H. sphondylium (H. sphondylium, H. sibiricum, H. montanum, H. ternatum, H. pyrenaicum subsp. pollinianum, H. pyrenaicum subsp. orsinii i H. verticillatum), i H. orphanidis, svi iz (tipske) sect. Heracleum, kao i H. austriacum subsp. siifolium iz sect. Wendia.
Rezultati i diskusija: Gasnom hromatografijom sa FID i MS detekcijom analiziran je sastav etarskih ulja (ukupno 59 uzoraka) izolovanih destilacijom vodenom parom iz podzemnih organa, listova, cvasti i plodova. U uljima podzemnih organa dominirali su monoterpeni i/ili fenilpropani (taksoni grupe H. sphondylium), ili (Z)-falkarinol (H. orphanidis i H. austriacum); u uljima plodova alifatični estri oktilacetat (sect. Heracleum) ili oktilheksanoat (H. austriacum), a u uljima listova i cvasti alifatični estri (H. orphanidis) ili seskviterpeni i/ili fenilpropani. Antimikrobna aktivnost 30 odabranih uzoraka etarskih ulja ispitivana je mikrodilucionom metodom na po osam sojeva bakterija i mikromiceta (u slučaju 17 uzoraka ulja MIK vrednosti su prema barem jednom mikroorganizmu bile niže ili bliske 100 µg/mL); selektivna citotoksičnost 26 uzoraka ulja MTT testom na tumorske HeLa, LS174 i A549 ćelije (u slučaju 13 uzoraka ulja IC50 vrednosti su prema barem jednoj ćelijskoj liniji bile niže ili bliske 30 µg/mL; ulja uglavnom nisu inhibirala rast normalnih MRC-5 ćelija). Ukupno 8 od ispitivanih 14 uzoraka ulja pokazalo je slab anti-DPPH potencijal. Sastav aktivnih zona u TLC-DPPH testu ispitan je gasnom hromatografijom (dominirali su oksidovani terpeni i fenilpropani). S obzirom da je u pojedinim uljima utvrđeno prisustvo furanokumarina koji mogu delovati fototoksično, definisan je bezbednosni profil ulja prema preporuci Evropske agencije za lekove (utvrđeno je da dnevni unos 1,94-15,68 mL ulja ne doprinosi značajno ukupnom riziku, koji postoji kao posledica unosa izvesnih količina furanokumarina hranom).
U dihlormetanskim ekstraktima podzemnih organa i plodova svih taksona identifikovano je ukupno 12 furanokumarina (LC-MS metodom pomoću standardnih supstanci ili na osnovu UV, MS, 1H i ROESY NMR spektara), od kojih su dominantni bili pimpinelin, bergapten, biakangelikol, heraklenin i/ili imperatorin. U uljastim supernatantima dihlormetanskih ekstrakata plodova gasnom hromatografijom (GC-FID i GC-MS) analizirane su masne kiseline (dominantna je bila petroselinska), steroli (najzastupljeniji je bio β-sitosterol) i triterpeni. 
Hemosistematski značaj komponenti etarskih ulja (u slučaju podzemnih organa i plodova i komponenti njihovih headspace frakcija) i furanokumarina utvrđen je multivarijantnom statističkom analizom (PCA, nMDS i UPGMA).
Zaključak: Sprovedena hemijska karakterizacija značajno je doprinela boljem poznavanju kompozicije sekundarnih i primarnih metabolita odabranih predstavnika sekcije Heracleum, dok su ispitivanja H. austriacum subsp. siifolium iz sekcije Wendia sprovedena po prvi put. Utvrđeno je da su ispitivani taksoni potencijalno novi biljni izvori jedinjenja značajnih za farmaceutsku, kozmetičku, prehrambenu ili hemijsku industriju. Rezultati ispitivanja farmakoloških aktivnosti etarskih ulja ukazali su na opravdanost daljih istraživanja ovih izolata kao potencijalno novih lekovitih biljnih sirovina, uz uslov poštovanja ustanovljenih maksimalnih dozvoljenih dnevnih unosa za ona etarska ulja u kojima su detektovani furanokumarini.
T1  - Chemical and pharmacological characterisation of 9 taxa of the genus Heracleum L. (Apiaceae), autochthonous for Southeastern Europe
T1  - Hemijska i farmakološka karakterizacija 9 taksona roda Heracleum L. (Apiaceae), autohtonih za jugoistočnu Evropu
UR  - https://hdl.handle.net/21.15107/rcub_farfar_3482
ER  - 

@misc{
author = "Ušjak, Ljuboš and Drobac, Milica and Soković, Marina and Stanojković, Tatjana and Niketić, Marjan and Petrović, Silvana",
year = "2019",
abstract = "Uvod: Predmet istraživanja je devet taksona roda Heracleum (Apiaceae): sedam iz grupe 
H. sphondylium (H. sphondylium, H. sibiricum, H. montanum, H. ternatum, H. pyrenaicum subsp. pollinianum, H. pyrenaicum subsp. orsinii i H. verticillatum), i H. orphanidis, svi iz (tipske) sect. Heracleum, kao i H. austriacum subsp. siifolium iz sect. Wendia.
Rezultati i diskusija: Gasnom hromatografijom sa FID i MS detekcijom analiziran je sastav etarskih ulja (ukupno 59 uzoraka) izolovanih destilacijom vodenom parom iz podzemnih organa, listova, cvasti i plodova. U uljima podzemnih organa dominirali su monoterpeni i/ili fenilpropani (taksoni grupe H. sphondylium), ili (Z)-falkarinol (H. orphanidis i H. austriacum); u uljima plodova alifatični estri oktilacetat (sect. Heracleum) ili oktilheksanoat (H. austriacum), a u uljima listova i cvasti alifatični estri (H. orphanidis) ili seskviterpeni i/ili fenilpropani. Antimikrobna aktivnost 30 odabranih uzoraka etarskih ulja ispitivana je mikrodilucionom metodom na po osam sojeva bakterija i mikromiceta (u slučaju 17 uzoraka ulja MIK vrednosti su prema barem jednom mikroorganizmu bile niže ili bliske 100 µg/mL); selektivna citotoksičnost 26 uzoraka ulja MTT testom na tumorske HeLa, LS174 i A549 ćelije (u slučaju 13 uzoraka ulja IC50 vrednosti su prema barem jednoj ćelijskoj liniji bile niže ili bliske 30 µg/mL; ulja uglavnom nisu inhibirala rast normalnih MRC-5 ćelija). Ukupno 8 od ispitivanih 14 uzoraka ulja pokazalo je slab anti-DPPH potencijal. Sastav aktivnih zona u TLC-DPPH testu ispitan je gasnom hromatografijom (dominirali su oksidovani terpeni i fenilpropani). S obzirom da je u pojedinim uljima utvrđeno prisustvo furanokumarina koji mogu delovati fototoksično, definisan je bezbednosni profil ulja prema preporuci Evropske agencije za lekove (utvrđeno je da dnevni unos 1,94-15,68 mL ulja ne doprinosi značajno ukupnom riziku, koji postoji kao posledica unosa izvesnih količina furanokumarina hranom).
U dihlormetanskim ekstraktima podzemnih organa i plodova svih taksona identifikovano je ukupno 12 furanokumarina (LC-MS metodom pomoću standardnih supstanci ili na osnovu UV, MS, 1H i ROESY NMR spektara), od kojih su dominantni bili pimpinelin, bergapten, biakangelikol, heraklenin i/ili imperatorin. U uljastim supernatantima dihlormetanskih ekstrakata plodova gasnom hromatografijom (GC-FID i GC-MS) analizirane su masne kiseline (dominantna je bila petroselinska), steroli (najzastupljeniji je bio β-sitosterol) i triterpeni. 
Hemosistematski značaj komponenti etarskih ulja (u slučaju podzemnih organa i plodova i komponenti njihovih headspace frakcija) i furanokumarina utvrđen je multivarijantnom statističkom analizom (PCA, nMDS i UPGMA).
Zaključak: Sprovedena hemijska karakterizacija značajno je doprinela boljem poznavanju kompozicije sekundarnih i primarnih metabolita odabranih predstavnika sekcije Heracleum, dok su ispitivanja H. austriacum subsp. siifolium iz sekcije Wendia sprovedena po prvi put. Utvrđeno je da su ispitivani taksoni potencijalno novi biljni izvori jedinjenja značajnih za farmaceutsku, kozmetičku, prehrambenu ili hemijsku industriju. Rezultati ispitivanja farmakoloških aktivnosti etarskih ulja ukazali su na opravdanost daljih istraživanja ovih izolata kao potencijalno novih lekovitih biljnih sirovina, uz uslov poštovanja ustanovljenih maksimalnih dozvoljenih dnevnih unosa za ona etarska ulja u kojima su detektovani furanokumarini.",
title = "Chemical and pharmacological characterisation of 9 taxa of the genus Heracleum L. (Apiaceae), autochthonous for Southeastern Europe, Hemijska i farmakološka karakterizacija 9 taksona roda Heracleum L. (Apiaceae), autohtonih za jugoistočnu Evropu",
url = "https://hdl.handle.net/21.15107/rcub_farfar_3482"
}

Ušjak, L., Drobac, M., Soković, M., Stanojković, T., Niketić, M.,& Petrović, S.. (2019). Chemical and pharmacological characterisation of 9 taxa of the genus Heracleum L. (Apiaceae), autochthonous for Southeastern Europe. .
https://hdl.handle.net/21.15107/rcub_farfar_3482

Ušjak L, Drobac M, Soković M, Stanojković T, Niketić M, Petrović S. Chemical and pharmacological characterisation of 9 taxa of the genus Heracleum L. (Apiaceae), autochthonous for Southeastern Europe. 2019;.
https://hdl.handle.net/21.15107/rcub_farfar_3482 .

Ušjak, Ljuboš, Drobac, Milica, Soković, Marina, Stanojković, Tatjana, Niketić, Marjan, Petrović, Silvana, "Chemical and pharmacological characterisation of 9 taxa of the genus Heracleum L. (Apiaceae), autochthonous for Southeastern Europe" (2019),
https://hdl.handle.net/21.15107/rcub_farfar_3482 .

TY  - CONF
AU  - Milutinović, Violeta
AU  - Ušjak, Ljuboš
AU  - Stanojković, Tatjana
AU  - Đorđić Crnogorac, Marija
AU  - Niketić, Marjan
AU  - Petrović, Silvana
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5258
AB  - Pyrus × velenovskyi Dostálek (Rosaceae) [P. pyraster (L.) Burgsd. × P. spinosa Forssk.] is a deciduous tree up to 5 m height. Leaf-blades 2.2–4.8 × 1.3–2.7 cm, ± glabrate, ellip tic, gradually narrowed to a rather long petiole. Th is natural hybrid plant was described from Bulgaria, and has been also found in Serbia. The objective of this work was to investigate the phenolic profile, as well as in vitro antioxidant, α-amylase and α-glucosidase inhibitory activities of dried methanol ex tract from the bark of this tree. The plant material was collected in eastern Serbia (Jelašnička Klisura gorge). In the dried bark, the contents of different classes of phenolics were spectrophotometrically determined: total polyphenols (10.36%), tannins (8.78%), procyanidins (4.21%) and phenolic glycosides (3.54%). After pre-extraction with dichloromethane, powdered bark was extracted with methanol by bimaceration at room temperature. In obtained dried methanol extract, using aforementioned spectrophotometric tests, the contents of total polyphenols (33.42%) and tannins (21.55%) were determined, and by HPLC, arbutin, chlorogenic acid, catechin and procyanidin B2 were identifi ed. Its antioxidant activity, i.e. ferric reducing capacity (FRAP), 2,2-diphenyl-1-picrylhydrazyl (DPPH) and OH radical scavenging ability, as well as the inhibition of the enzymes α-amylase and α-glucosidase were assessed using corresponding colorimetric assays. Tested dried methanol extract exhibited signifi cant DPPH and OH radical scavenging abilities (SC50 = 6.85 and 12.21 μg/mL, respectively), and ferric reducing capacity (10.74 mmol Fe (II)/g of dried extract), as well as the inhibition of α-amylase (IC50 = 11.4 μg/mL) and α-glucosidase (IC50 = 5.48 μg/mL).
PB  - Institute of Botany and Botanical Garden Jevremovac, Belgrade
C3  - Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts
T1  - Phenolic constituents, antioxidant, α-amylase and α-glucosidase inhibitory activities of Pyrus × velenovskyi bark
VL  - 42
IS  - 1 supplement
SP  - 139
EP  - 139
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5258
ER  - 

@conference{
author = "Milutinović, Violeta and Ušjak, Ljuboš and Stanojković, Tatjana and Đorđić Crnogorac, Marija and Niketić, Marjan and Petrović, Silvana",
year = "2018",
abstract = "Pyrus × velenovskyi Dostálek (Rosaceae) [P. pyraster (L.) Burgsd. × P. spinosa Forssk.] is a deciduous tree up to 5 m height. Leaf-blades 2.2–4.8 × 1.3–2.7 cm, ± glabrate, ellip tic, gradually narrowed to a rather long petiole. Th is natural hybrid plant was described from Bulgaria, and has been also found in Serbia. The objective of this work was to investigate the phenolic profile, as well as in vitro antioxidant, α-amylase and α-glucosidase inhibitory activities of dried methanol ex tract from the bark of this tree. The plant material was collected in eastern Serbia (Jelašnička Klisura gorge). In the dried bark, the contents of different classes of phenolics were spectrophotometrically determined: total polyphenols (10.36%), tannins (8.78%), procyanidins (4.21%) and phenolic glycosides (3.54%). After pre-extraction with dichloromethane, powdered bark was extracted with methanol by bimaceration at room temperature. In obtained dried methanol extract, using aforementioned spectrophotometric tests, the contents of total polyphenols (33.42%) and tannins (21.55%) were determined, and by HPLC, arbutin, chlorogenic acid, catechin and procyanidin B2 were identifi ed. Its antioxidant activity, i.e. ferric reducing capacity (FRAP), 2,2-diphenyl-1-picrylhydrazyl (DPPH) and OH radical scavenging ability, as well as the inhibition of the enzymes α-amylase and α-glucosidase were assessed using corresponding colorimetric assays. Tested dried methanol extract exhibited signifi cant DPPH and OH radical scavenging abilities (SC50 = 6.85 and 12.21 μg/mL, respectively), and ferric reducing capacity (10.74 mmol Fe (II)/g of dried extract), as well as the inhibition of α-amylase (IC50 = 11.4 μg/mL) and α-glucosidase (IC50 = 5.48 μg/mL).",
publisher = "Institute of Botany and Botanical Garden Jevremovac, Belgrade",
journal = "Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts",
title = "Phenolic constituents, antioxidant, α-amylase and α-glucosidase inhibitory activities of Pyrus × velenovskyi bark",
volume = "42",
number = "1 supplement",
pages = "139-139",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5258"
}

Milutinović, V., Ušjak, L., Stanojković, T., Đorđić Crnogorac, M., Niketić, M.,& Petrović, S.. (2018). Phenolic constituents, antioxidant, α-amylase and α-glucosidase inhibitory activities of Pyrus × velenovskyi bark. in Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts
Institute of Botany and Botanical Garden Jevremovac, Belgrade., 42(1 supplement), 139-139.
https://hdl.handle.net/21.15107/rcub_farfar_5258

Milutinović V, Ušjak L, Stanojković T, Đorđić Crnogorac M, Niketić M, Petrović S. Phenolic constituents, antioxidant, α-amylase and α-glucosidase inhibitory activities of Pyrus × velenovskyi bark. in Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts. 2018;42(1 supplement):139-139.
https://hdl.handle.net/21.15107/rcub_farfar_5258 .

Milutinović, Violeta, Ušjak, Ljuboš, Stanojković, Tatjana, Đorđić Crnogorac, Marija, Niketić, Marjan, Petrović, Silvana, "Phenolic constituents, antioxidant, α-amylase and α-glucosidase inhibitory activities of Pyrus × velenovskyi bark" in Botanica Serbica, The 7th Balkan Botanical Congress – 7BBC 2018, Book of abstracts, 42, no. 1 supplement (2018):139-139,
https://hdl.handle.net/21.15107/rcub_farfar_5258 .

TY  - THES
AU  - Damjanović, Ana B.
PY  - 2018
UR  - http://nardus.mpn.gov.rs/handle/123456789/9479
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=5790
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:17663/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=50063119
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3674
AB  - Na osnovu podataka o upotrebi vrste Mahonia aquifolium u tradicionalnoj medicini definisan je cilj našeg istraživanja, ispitivanje antitumorske i antimikrobne aktivnosti ekstrakata kore mahonije.Ispitivanjem citotoksičnosti vodenog i etanolnog ekstrakta na humanim malignim ćelijskim linijama je dokazano da ekstrakti imaju selektivnu citotoksičnu aktivnost, a najviši intenzitet dejstva su pokazali na HeLa ćelijama adenokarcinoma cerviksa. Fluorescentnom mikroskopijom i analizom faza ćelijskog ciklusa je pokazano da ekstrakti indukuju apoptozu u HeLa ćelijama. Primenom inhibitora kaspaza pokazano je da ekstrakti indukuju apoptozu preko spoljašnjeg puta aktivacije. Ispitivanja na endotelijalnim EA.hy926 ćelijama pokazala su antiangiogenetski i antimigratorni efekat ekstrakata.Primena tretmana doksorubicin/ekstrakt ukazala je na izraženo sinergističko dejstvo ekstrakta sa doksorubicinom na A549 ćelijama karcinoma pluća. Ekstrakti povećavaju zadržavanje leka u ćeliji i pojačavaju efekat doksorubicina na smanjenje migracije A549 ćelija i njihovo grupisanje u kolonije. Antimetastatski potencijal kombinacije doksorubicin/ekstrakt potvrđen je analizom ekspresije gena odgovornih za invazivnost.Oba ekstrakta ispoljavaju antimikrobni efekat, koji je najizraženiji na bakterijskoj vrsti Staphylococcus aureus. Ekstrakti dovode do smanjenja produkcije biofilma i glikokaliksa. Sa antibioticima i antimikoticima ekstrakti su delovali sinergistički.Hemijskom analizom potvrđeno je da su za aktivnost ekstrakata M. aquifolium odgovorni alkaloidi tipa berberina. Na intenzitet citotoksičnosti najviše utiču berberin, palmatin i berbamin.Rezultati su pokazali da M. aquifolium može naći svoje mesto u terapiji malignih ili infektivnih oboljenja, pojedinačno i u kombinaciji sa postojećim lekovima.
AB  - The goal of our research was investigation of antitumor and antimicrobial activities of Mahonia aquifolium extracts and it was based on its use in traditional medicine. Water and ethanol extract exerted selective cytotoxic activity on examined malignant cell lines, and the best activity was observed against cervical adenocarcinoma, HeLa, cells. Fluorescence microscopy and cell cycle analysis showed that both extracts induce apoptosis in HeLa cells. Experiments with caspase inhibitors showed that extracts induce a caspase-dependent apoptosis through extrinsic pathway. Both extracts inhibit the migration of EA.hy926 cells, and exerted anti-angiogenic effect.The investigation of the doxorubicine/extract treatment showed synergistic effect of extracts with doxorubicine against lung carcinoma A549, cells. Extracts were shown to prolong doxorubicin retention time in A549 cells. A549 cells treated with doxorubicin/extract were shown to have lower migratory and colony formation potentials than the cells treated with doxorubicin alone. Antimetastatic effect of the treatment was confirmed with analysis of expression of genes responsible for malignant cells invasiveness.Extracts exerted antimicrobial effect. The best effect was observed against Staphylococcus aureus. Extracts showed potential to inhibit production of biofilm and glycocalycs. The use of extracts with antibiotics and antimycotics showed synergistic effect.Chemical analysis confirmed that berberine type alkaloids are the most responsible for activities of M. aquifolium. The compounds with the highest cytotoxic activity are: berberine, palmatine, and berbamine.The results of our research suggested that M. aquifolium may be used in therapy of malignant or infective diseases, alone or in combination with other drugs.
PB  - Универзитет у Београду, Фармацеутски факултет
T2  - Универзитет у Београду
T1  - In vitro ispitivanje antitumorske i antimikrobne aktivnosti ekstrakata mahonije, Mahonia aquifolium (Pursh) Nutt.
UR  - https://hdl.handle.net/21.15107/rcub_nardus_9479
ER  - 

@phdthesis{
author = "Damjanović, Ana B.",
year = "2018",
abstract = "Na osnovu podataka o upotrebi vrste Mahonia aquifolium u tradicionalnoj medicini definisan je cilj našeg istraživanja, ispitivanje antitumorske i antimikrobne aktivnosti ekstrakata kore mahonije.Ispitivanjem citotoksičnosti vodenog i etanolnog ekstrakta na humanim malignim ćelijskim linijama je dokazano da ekstrakti imaju selektivnu citotoksičnu aktivnost, a najviši intenzitet dejstva su pokazali na HeLa ćelijama adenokarcinoma cerviksa. Fluorescentnom mikroskopijom i analizom faza ćelijskog ciklusa je pokazano da ekstrakti indukuju apoptozu u HeLa ćelijama. Primenom inhibitora kaspaza pokazano je da ekstrakti indukuju apoptozu preko spoljašnjeg puta aktivacije. Ispitivanja na endotelijalnim EA.hy926 ćelijama pokazala su antiangiogenetski i antimigratorni efekat ekstrakata.Primena tretmana doksorubicin/ekstrakt ukazala je na izraženo sinergističko dejstvo ekstrakta sa doksorubicinom na A549 ćelijama karcinoma pluća. Ekstrakti povećavaju zadržavanje leka u ćeliji i pojačavaju efekat doksorubicina na smanjenje migracije A549 ćelija i njihovo grupisanje u kolonije. Antimetastatski potencijal kombinacije doksorubicin/ekstrakt potvrđen je analizom ekspresije gena odgovornih za invazivnost.Oba ekstrakta ispoljavaju antimikrobni efekat, koji je najizraženiji na bakterijskoj vrsti Staphylococcus aureus. Ekstrakti dovode do smanjenja produkcije biofilma i glikokaliksa. Sa antibioticima i antimikoticima ekstrakti su delovali sinergistički.Hemijskom analizom potvrđeno je da su za aktivnost ekstrakata M. aquifolium odgovorni alkaloidi tipa berberina. Na intenzitet citotoksičnosti najviše utiču berberin, palmatin i berbamin.Rezultati su pokazali da M. aquifolium može naći svoje mesto u terapiji malignih ili infektivnih oboljenja, pojedinačno i u kombinaciji sa postojećim lekovima., The goal of our research was investigation of antitumor and antimicrobial activities of Mahonia aquifolium extracts and it was based on its use in traditional medicine. Water and ethanol extract exerted selective cytotoxic activity on examined malignant cell lines, and the best activity was observed against cervical adenocarcinoma, HeLa, cells. Fluorescence microscopy and cell cycle analysis showed that both extracts induce apoptosis in HeLa cells. Experiments with caspase inhibitors showed that extracts induce a caspase-dependent apoptosis through extrinsic pathway. Both extracts inhibit the migration of EA.hy926 cells, and exerted anti-angiogenic effect.The investigation of the doxorubicine/extract treatment showed synergistic effect of extracts with doxorubicine against lung carcinoma A549, cells. Extracts were shown to prolong doxorubicin retention time in A549 cells. A549 cells treated with doxorubicin/extract were shown to have lower migratory and colony formation potentials than the cells treated with doxorubicin alone. Antimetastatic effect of the treatment was confirmed with analysis of expression of genes responsible for malignant cells invasiveness.Extracts exerted antimicrobial effect. The best effect was observed against Staphylococcus aureus. Extracts showed potential to inhibit production of biofilm and glycocalycs. The use of extracts with antibiotics and antimycotics showed synergistic effect.Chemical analysis confirmed that berberine type alkaloids are the most responsible for activities of M. aquifolium. The compounds with the highest cytotoxic activity are: berberine, palmatine, and berbamine.The results of our research suggested that M. aquifolium may be used in therapy of malignant or infective diseases, alone or in combination with other drugs.",
publisher = "Универзитет у Београду, Фармацеутски факултет",
journal = "Универзитет у Београду",
title = "In vitro ispitivanje antitumorske i antimikrobne aktivnosti ekstrakata mahonije, Mahonia aquifolium (Pursh) Nutt.",
url = "https://hdl.handle.net/21.15107/rcub_nardus_9479"
}

Damjanović, A. B.. (2018). In vitro ispitivanje antitumorske i antimikrobne aktivnosti ekstrakata mahonije, Mahonia aquifolium (Pursh) Nutt.. in Универзитет у Београду
Универзитет у Београду, Фармацеутски факултет..
https://hdl.handle.net/21.15107/rcub_nardus_9479

Damjanović AB. In vitro ispitivanje antitumorske i antimikrobne aktivnosti ekstrakata mahonije, Mahonia aquifolium (Pursh) Nutt.. in Универзитет у Београду. 2018;.
https://hdl.handle.net/21.15107/rcub_nardus_9479 .

Damjanović, Ana B., "In vitro ispitivanje antitumorske i antimikrobne aktivnosti ekstrakata mahonije, Mahonia aquifolium (Pursh) Nutt." in Универзитет у Београду (2018),
https://hdl.handle.net/21.15107/rcub_nardus_9479 .

TY  - JOUR
AU  - Milović, Sanja
AU  - Kundaković, Tatjana
AU  - Macić, Vesna
AU  - Antić-Stanković, Jelena
AU  - Grozdanić, Nada
AU  - Đuričić, Ivana
AU  - Stanković, Ivan
AU  - Stanojković, Tatjana
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2866
AB  - The aim of the present study was to reveal the potential biological activities of the dichlorometane: methanol (1:1) dry extract (DME) and the nutritional value of selected seaweeds: Cymodocea nodosa, Halimeda tuna, Cystoseira barbata and Codium bursa, collected from the Adriatic coast, Montenegro. We assessed the chemical composition and several biological activities such as: anti-a-glucosidase, antitumour, antimicrobial and antioxidative activity. H. tuna had the best cytotoxic activity against human colon carcinoma cell line, LS174 (IC50 = 17.92 +/- 1.54 mu g/mL). C. nodosa demonstrated strong cytotoxicity against human adenocarcinoma cell line, HeLa (IC50 = 13.28 +/- 0.39 mu g/mL) and human chronic myelogenous leukaemia cell line, K562 (IC50 = 19.64 +/- 1.55 mu g/mL). C. barbata had the best anti a-glucosidase (IC50 = 9.98 +/- 3.34 mu g/mL) and antimicrobial activity (minimal inhibitory concentration of 100 mu g/mL) for Staphylococcus aureus and Bacillus subtilis. C. bursa showed the highest nutritional value (490.4 kcal).
PB  - Soc Stinte Farmaceutice Romania, Bucuresti
T2  - Farmacia
T1  - Anti α-glucosidase, antitumour, antioxidative, antimicrobial activity, nutritive and health protective potential of some seaweeds from the Adriatic coast of Montenegro
VL  - 65
IS  - 5
SP  - 731
EP  - 740
UR  - https://hdl.handle.net/21.15107/rcub_farfar_2866
ER  - 

@article{
author = "Milović, Sanja and Kundaković, Tatjana and Macić, Vesna and Antić-Stanković, Jelena and Grozdanić, Nada and Đuričić, Ivana and Stanković, Ivan and Stanojković, Tatjana",
year = "2017",
abstract = "The aim of the present study was to reveal the potential biological activities of the dichlorometane: methanol (1:1) dry extract (DME) and the nutritional value of selected seaweeds: Cymodocea nodosa, Halimeda tuna, Cystoseira barbata and Codium bursa, collected from the Adriatic coast, Montenegro. We assessed the chemical composition and several biological activities such as: anti-a-glucosidase, antitumour, antimicrobial and antioxidative activity. H. tuna had the best cytotoxic activity against human colon carcinoma cell line, LS174 (IC50 = 17.92 +/- 1.54 mu g/mL). C. nodosa demonstrated strong cytotoxicity against human adenocarcinoma cell line, HeLa (IC50 = 13.28 +/- 0.39 mu g/mL) and human chronic myelogenous leukaemia cell line, K562 (IC50 = 19.64 +/- 1.55 mu g/mL). C. barbata had the best anti a-glucosidase (IC50 = 9.98 +/- 3.34 mu g/mL) and antimicrobial activity (minimal inhibitory concentration of 100 mu g/mL) for Staphylococcus aureus and Bacillus subtilis. C. bursa showed the highest nutritional value (490.4 kcal).",
publisher = "Soc Stinte Farmaceutice Romania, Bucuresti",
journal = "Farmacia",
title = "Anti α-glucosidase, antitumour, antioxidative, antimicrobial activity, nutritive and health protective potential of some seaweeds from the Adriatic coast of Montenegro",
volume = "65",
number = "5",
pages = "731-740",
url = "https://hdl.handle.net/21.15107/rcub_farfar_2866"
}

Milović, S., Kundaković, T., Macić, V., Antić-Stanković, J., Grozdanić, N., Đuričić, I., Stanković, I.,& Stanojković, T.. (2017). Anti α-glucosidase, antitumour, antioxidative, antimicrobial activity, nutritive and health protective potential of some seaweeds from the Adriatic coast of Montenegro. in Farmacia
Soc Stinte Farmaceutice Romania, Bucuresti., 65(5), 731-740.
https://hdl.handle.net/21.15107/rcub_farfar_2866

Milović S, Kundaković T, Macić V, Antić-Stanković J, Grozdanić N, Đuričić I, Stanković I, Stanojković T. Anti α-glucosidase, antitumour, antioxidative, antimicrobial activity, nutritive and health protective potential of some seaweeds from the Adriatic coast of Montenegro. in Farmacia. 2017;65(5):731-740.
https://hdl.handle.net/21.15107/rcub_farfar_2866 .

Milović, Sanja, Kundaković, Tatjana, Macić, Vesna, Antić-Stanković, Jelena, Grozdanić, Nada, Đuričić, Ivana, Stanković, Ivan, Stanojković, Tatjana, "Anti α-glucosidase, antitumour, antioxidative, antimicrobial activity, nutritive and health protective potential of some seaweeds from the Adriatic coast of Montenegro" in Farmacia, 65, no. 5 (2017):731-740,
https://hdl.handle.net/21.15107/rcub_farfar_2866 .

TY  - JOUR
AU  - Ušjak, Ljuboš
AU  - Petrović, Silvana
AU  - Drobac, Milica
AU  - Soković, Marina
AU  - Stanojković, Tatjana
AU  - Ćirić, Ana D.
AU  - Niketić, Marjan
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3000
AB  - Many Heracleum L. taxa (Apiaceae) are used as food and spices, and in traditional medicine. In this work, the chemical composition of Heracleum pyrenaicum subsp. orsinii (Guss.) F. Pedrotti and Pignatti root, leaf and fruit essential oils, their antimicrobial activity and cytotoxic effect on malignant and normal cells were investigated for the first time. The composition of the oils was analyzed by GC and GC-MS. Monoterpenes prevailed in the root oil, with beta-pinene (38.6%) being dominant, while in the leaf oil, sesquiterpenes, mostly (E)-nerolidol (20.5%) and (E)-caryophyllene (17.0%), were the most abundant constituents. The fruit oil contained the majority of aliphatic esters, mainly octyl acetate (36.8%) and octyl hexanoate (22.1%). The antimicrobial activity was determined by microdilution method against eight bacteria and eight fungi (standard strains, clinical or food isolates). The best antibacterial activity, better than the activity of ampicillin, was shown by the root oil against Salmonella typhimurium, Escherichia coli and Pseudomonas aeruginosa. The strongest antifungal activity, stronger than the activity of ketoconazole, was exhibited by the leaf and root oils against Trichoderma viride, and by the root oil against Aspergillus ochraceus. The cytotoxic effect of the oils, determined by MTT test, was prominent against malignant HeLa, LS174 and A549 cells (IC50 = 6.49-14.56 µg/mL). On the other hand, the oils did not show toxicity against normal MRC-5 cells at tested concentrations (IC50 > 200.00 µg/mL). It can be concluded that investigated H. pyrenaicum subsp. orsinii oils represent potential new raw materials for food and pharmaceutical industry.
PB  - Springer India, New Delhi
T2  - Journal of Food Science and Technology-Mysore
T1  - Edible wild plant Heracleum pyrenaicum subsp orsinii as a potential new source of bioactive essential oils
VL  - 54
IS  - 8
SP  - 2193
EP  - 2202
DO  - 10.1007/s13197-017-2610-z
ER  - 

@article{
author = "Ušjak, Ljuboš and Petrović, Silvana and Drobac, Milica and Soković, Marina and Stanojković, Tatjana and Ćirić, Ana D. and Niketić, Marjan",
year = "2017",
abstract = "Many Heracleum L. taxa (Apiaceae) are used as food and spices, and in traditional medicine. In this work, the chemical composition of Heracleum pyrenaicum subsp. orsinii (Guss.) F. Pedrotti and Pignatti root, leaf and fruit essential oils, their antimicrobial activity and cytotoxic effect on malignant and normal cells were investigated for the first time. The composition of the oils was analyzed by GC and GC-MS. Monoterpenes prevailed in the root oil, with beta-pinene (38.6%) being dominant, while in the leaf oil, sesquiterpenes, mostly (E)-nerolidol (20.5%) and (E)-caryophyllene (17.0%), were the most abundant constituents. The fruit oil contained the majority of aliphatic esters, mainly octyl acetate (36.8%) and octyl hexanoate (22.1%). The antimicrobial activity was determined by microdilution method against eight bacteria and eight fungi (standard strains, clinical or food isolates). The best antibacterial activity, better than the activity of ampicillin, was shown by the root oil against Salmonella typhimurium, Escherichia coli and Pseudomonas aeruginosa. The strongest antifungal activity, stronger than the activity of ketoconazole, was exhibited by the leaf and root oils against Trichoderma viride, and by the root oil against Aspergillus ochraceus. The cytotoxic effect of the oils, determined by MTT test, was prominent against malignant HeLa, LS174 and A549 cells (IC50 = 6.49-14.56 µg/mL). On the other hand, the oils did not show toxicity against normal MRC-5 cells at tested concentrations (IC50 > 200.00 µg/mL). It can be concluded that investigated H. pyrenaicum subsp. orsinii oils represent potential new raw materials for food and pharmaceutical industry.",
publisher = "Springer India, New Delhi",
journal = "Journal of Food Science and Technology-Mysore",
title = "Edible wild plant Heracleum pyrenaicum subsp orsinii as a potential new source of bioactive essential oils",
volume = "54",
number = "8",
pages = "2193-2202",
doi = "10.1007/s13197-017-2610-z"
}

Ušjak, L., Petrović, S., Drobac, M., Soković, M., Stanojković, T., Ćirić, A. D.,& Niketić, M.. (2017). Edible wild plant Heracleum pyrenaicum subsp orsinii as a potential new source of bioactive essential oils. in Journal of Food Science and Technology-Mysore
Springer India, New Delhi., 54(8), 2193-2202.
https://doi.org/10.1007/s13197-017-2610-z

Ušjak L, Petrović S, Drobac M, Soković M, Stanojković T, Ćirić AD, Niketić M. Edible wild plant Heracleum pyrenaicum subsp orsinii as a potential new source of bioactive essential oils. in Journal of Food Science and Technology-Mysore. 2017;54(8):2193-2202.
doi:10.1007/s13197-017-2610-z .

Ušjak, Ljuboš, Petrović, Silvana, Drobac, Milica, Soković, Marina, Stanojković, Tatjana, Ćirić, Ana D., Niketić, Marjan, "Edible wild plant Heracleum pyrenaicum subsp orsinii as a potential new source of bioactive essential oils" in Journal of Food Science and Technology-Mysore, 54, no. 8 (2017):2193-2202,
https://doi.org/10.1007/s13197-017-2610-z . .

TY  - JOUR
AU  - Ušjak, Ljuboš
AU  - Petrović, Silvana
AU  - Drobac, Milica
AU  - Soković, Marina
AU  - Stanojković, Tatjana
AU  - Ćirić, Ana D.
AU  - Niketić, Marjan
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2908
AB  - Although some widespread, native cow parsnips (Heracleum L. spp., Apiaceae) had broad medicinal and culinary applications throughout history, the knowledge about their volatile constituents is insufficient. This work investigates the composition and bioactivities of H. sphondylium L. (HSPH), H. sibiricum L. (HSIB) and H. montanum Schleich. ex Gaudin (HMON) essential oils. The composition was tested by GC and GC-MS. (Z)-β-Ocimene was the most abundant in HSPH (28.9%) and HMON (20.4%) root oils, while in HSIB root oil, β-pinene (26.2%), methyl eugenol (22.3%) and elemicin (25.6%) prevailed. Leaf and flower oils were dominated by various sesquiterpenes (germacrene D, β-sesquiphellandrene, (E)-β-farnesene and/or (E)-caryophyllene) and/or phenylpropanoids (apiole, methyl eugenol, elemicin and/or (Z)-isoelemicin). Octyl acetate (57.5-67.1%) was the main constituent of all fruit oils. The antimicrobial activity was screened by a microdilution method against eight bacteria and eight fungi. The strongest antimicrobial effect, in several cases better than the activity of antibiotics, was shown by HSPH (MICs = 0.12-3.30 mg mL-1) and HMON (MICs = 0.10-1.30 mg mL-1) flower oils against bacteria, and HSIB fruit oil against fungi (MICs = 0.15-0.40 mg mL-1). The MTT test revealed that the oils were not or weakly cytotoxic against human malignant HeLa, LS174 and/or A549 cells (except HSPH root oil; IC50 = 5.72-24.31 μg mL-1) and that tested oils were not toxic against human normal MRC-5 cells (at 200.00 μg mL-1). Significant activity observed against microorganisms that are the common cause of foodborne diseases, food contamination and/or hospital-acquired infections justifies certain traditional uses of the investigated plants and represents a good basis for further research of these Heracleum oils.
AB  - 
PB  - Royal Soc Chemistry, Cambridge
T2  - Food & Function
T1  - Essential oils of three cow parsnips - composition and activity against nosocomial and foodborne pathogens and food contaminants
VL  - 8
IS  - 1
SP  - 278
EP  - 290
DO  - 10.1039/c6fo01698g
ER  - 

@article{
author = "Ušjak, Ljuboš and Petrović, Silvana and Drobac, Milica and Soković, Marina and Stanojković, Tatjana and Ćirić, Ana D. and Niketić, Marjan",
year = "2017",
abstract = "Although some widespread, native cow parsnips (Heracleum L. spp., Apiaceae) had broad medicinal and culinary applications throughout history, the knowledge about their volatile constituents is insufficient. This work investigates the composition and bioactivities of H. sphondylium L. (HSPH), H. sibiricum L. (HSIB) and H. montanum Schleich. ex Gaudin (HMON) essential oils. The composition was tested by GC and GC-MS. (Z)-β-Ocimene was the most abundant in HSPH (28.9%) and HMON (20.4%) root oils, while in HSIB root oil, β-pinene (26.2%), methyl eugenol (22.3%) and elemicin (25.6%) prevailed. Leaf and flower oils were dominated by various sesquiterpenes (germacrene D, β-sesquiphellandrene, (E)-β-farnesene and/or (E)-caryophyllene) and/or phenylpropanoids (apiole, methyl eugenol, elemicin and/or (Z)-isoelemicin). Octyl acetate (57.5-67.1%) was the main constituent of all fruit oils. The antimicrobial activity was screened by a microdilution method against eight bacteria and eight fungi. The strongest antimicrobial effect, in several cases better than the activity of antibiotics, was shown by HSPH (MICs = 0.12-3.30 mg mL-1) and HMON (MICs = 0.10-1.30 mg mL-1) flower oils against bacteria, and HSIB fruit oil against fungi (MICs = 0.15-0.40 mg mL-1). The MTT test revealed that the oils were not or weakly cytotoxic against human malignant HeLa, LS174 and/or A549 cells (except HSPH root oil; IC50 = 5.72-24.31 μg mL-1) and that tested oils were not toxic against human normal MRC-5 cells (at 200.00 μg mL-1). Significant activity observed against microorganisms that are the common cause of foodborne diseases, food contamination and/or hospital-acquired infections justifies certain traditional uses of the investigated plants and represents a good basis for further research of these Heracleum oils., ",
publisher = "Royal Soc Chemistry, Cambridge",
journal = "Food & Function",
title = "Essential oils of three cow parsnips - composition and activity against nosocomial and foodborne pathogens and food contaminants",
volume = "8",
number = "1",
pages = "278-290",
doi = "10.1039/c6fo01698g"
}

Ušjak, L., Petrović, S., Drobac, M., Soković, M., Stanojković, T., Ćirić, A. D.,& Niketić, M.. (2017). Essential oils of three cow parsnips - composition and activity against nosocomial and foodborne pathogens and food contaminants. in Food & Function
Royal Soc Chemistry, Cambridge., 8(1), 278-290.
https://doi.org/10.1039/c6fo01698g

Ušjak L, Petrović S, Drobac M, Soković M, Stanojković T, Ćirić AD, Niketić M. Essential oils of three cow parsnips - composition and activity against nosocomial and foodborne pathogens and food contaminants. in Food & Function. 2017;8(1):278-290.
doi:10.1039/c6fo01698g .

Ušjak, Ljuboš, Petrović, Silvana, Drobac, Milica, Soković, Marina, Stanojković, Tatjana, Ćirić, Ana D., Niketić, Marjan, "Essential oils of three cow parsnips - composition and activity against nosocomial and foodborne pathogens and food contaminants" in Food & Function, 8, no. 1 (2017):278-290,
https://doi.org/10.1039/c6fo01698g . .

TY  - JOUR
AU  - Ušjak, Ljuboš
AU  - Petrović, Silvana
AU  - Drobac, Milica
AU  - Soković, Marina
AU  - Stanojković, Tatjana
AU  - Ćirić, Ana D.
AU  - Niketić, Marjan
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2571
AB  - The objective of this research was to analyze the chemical composition, antimicrobial and cytotoxic activity of Heracleum pyrenaicum subsp. pollinianum
(Bertol.) F. Pedrotti & Pignatti (HPP) and H. orphanidis Boiss. (HO) essential oils. The composition of the oils was analyzed by GC and GC-MS. β-Pinene
(35.1%) was the most abundant compound in HPP root oil, while (Z)-falcarinol (80.0%) dominated in HO root oil. (E)-Nerolidol (28.5%) was the main
constituent in HPP leaf oil. HPP fruit oil, as well as HO leaf and fruit oils mainly contained aliphatic esters, mostly octyl acetate (50.5-84.5%). Antimicrobial
screening was performed by microdilution method against eight bacterial and eight fungal strains. The strongest antibacterial activity was shown by both root
oils (MICs 0.02-0.60 mg/mL and MBCs 0.04-2.50 mg/mL for HPP, and MICs 0.02-1.25 mg/mL and MBCs 0.04-2.50 mg/mL for HO), while the best
antifungal potential was exhibited by HPP fruit oil (MICs 0.30-0.60 mg/mL and MFCs 0.60-1.25 mg/mL) and HO leaf oil (MICs 0.15-0.63 mg/mL and MFCs
0.30-1.25 mg/mL). The tested root and fruit oils exhibited strong cytotoxic effect, which was determined by MTT test against HeLa (IC50 7.53-21.07 μg/mL)
and LS174 (IC50 24.16-58.86 μg/mL) cell lines.
PB  - Natural Products Inc, Westerville
T2  - Natural Product Communications
T1  - Chemical Composition and Bioactivity of the Essential Oils of Heracleum pyrenaicum subsp. pollinianum and Heracleum orphanidis
VL  - 11
IS  - 4
SP  - 529
EP  - 534
UR  - https://hdl.handle.net/21.15107/rcub_ibiss_2990
ER  - 

@article{
author = "Ušjak, Ljuboš and Petrović, Silvana and Drobac, Milica and Soković, Marina and Stanojković, Tatjana and Ćirić, Ana D. and Niketić, Marjan",
year = "2016",
abstract = "The objective of this research was to analyze the chemical composition, antimicrobial and cytotoxic activity of Heracleum pyrenaicum subsp. pollinianum
(Bertol.) F. Pedrotti & Pignatti (HPP) and H. orphanidis Boiss. (HO) essential oils. The composition of the oils was analyzed by GC and GC-MS. β-Pinene
(35.1%) was the most abundant compound in HPP root oil, while (Z)-falcarinol (80.0%) dominated in HO root oil. (E)-Nerolidol (28.5%) was the main
constituent in HPP leaf oil. HPP fruit oil, as well as HO leaf and fruit oils mainly contained aliphatic esters, mostly octyl acetate (50.5-84.5%). Antimicrobial
screening was performed by microdilution method against eight bacterial and eight fungal strains. The strongest antibacterial activity was shown by both root
oils (MICs 0.02-0.60 mg/mL and MBCs 0.04-2.50 mg/mL for HPP, and MICs 0.02-1.25 mg/mL and MBCs 0.04-2.50 mg/mL for HO), while the best
antifungal potential was exhibited by HPP fruit oil (MICs 0.30-0.60 mg/mL and MFCs 0.60-1.25 mg/mL) and HO leaf oil (MICs 0.15-0.63 mg/mL and MFCs
0.30-1.25 mg/mL). The tested root and fruit oils exhibited strong cytotoxic effect, which was determined by MTT test against HeLa (IC50 7.53-21.07 μg/mL)
and LS174 (IC50 24.16-58.86 μg/mL) cell lines.",
publisher = "Natural Products Inc, Westerville",
journal = "Natural Product Communications",
title = "Chemical Composition and Bioactivity of the Essential Oils of Heracleum pyrenaicum subsp. pollinianum and Heracleum orphanidis",
volume = "11",
number = "4",
pages = "529-534",
url = "https://hdl.handle.net/21.15107/rcub_ibiss_2990"
}

Ušjak, L., Petrović, S., Drobac, M., Soković, M., Stanojković, T., Ćirić, A. D.,& Niketić, M.. (2016). Chemical Composition and Bioactivity of the Essential Oils of Heracleum pyrenaicum subsp. pollinianum and Heracleum orphanidis. in Natural Product Communications
Natural Products Inc, Westerville., 11(4), 529-534.
https://hdl.handle.net/21.15107/rcub_ibiss_2990

Ušjak L, Petrović S, Drobac M, Soković M, Stanojković T, Ćirić AD, Niketić M. Chemical Composition and Bioactivity of the Essential Oils of Heracleum pyrenaicum subsp. pollinianum and Heracleum orphanidis. in Natural Product Communications. 2016;11(4):529-534.
https://hdl.handle.net/21.15107/rcub_ibiss_2990 .

Ušjak, Ljuboš, Petrović, Silvana, Drobac, Milica, Soković, Marina, Stanojković, Tatjana, Ćirić, Ana D., Niketić, Marjan, "Chemical Composition and Bioactivity of the Essential Oils of Heracleum pyrenaicum subsp. pollinianum and Heracleum orphanidis" in Natural Product Communications, 11, no. 4 (2016):529-534,
https://hdl.handle.net/21.15107/rcub_ibiss_2990 .

TY  - JOUR
AU  - Ušjak, Ljuboš
AU  - Petrović, Silvana
AU  - Drobac, Milica
AU  - Soković, Marina
AU  - Stanojković, Tatjana
AU  - Ćirić, Ana D.
AU  - Grozdanić, Nađa
AU  - Niketić, Marjan
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2534
AB  - In this work, the chemical composition, antimicrobial and cytotoxic activity of Heracleum verticillatum Pančic and H. ternatum Velen. root, leaf, and fruit essential oils were investigated. The composition was analyzed by GC and GC/MS. Heracleum verticillatum and H. ternatum root oils were dominated by monoterpenes, mostly β-pinene (23.5% and 47.3%, respectively). Heracleum verticillatum leaf oil was characterized by monoterpenes, mainly limonene (20.3%), and sesquiterpenes, mostly (E)-caryophyllene (19.1%), while H. ternatum leaf oil by the high percentage of phenylpropanoids, with (Z)-isoelemicin (35.1%) being dominant constituent. Both fruit oils contained the majority of aliphatic esters, mostly octyl acetate (42.3% in H. verticillatum oil and 49.0% in H. ternatum oil). The antimicrobial activity of the oils was determined by microdilution method against eight bacterial and eight fungal strains. The strongest effect was exhibited by H. verticillatum root oil, particularly against Staphylococcus aureus, Salmonella typhimurium (MICs = 0.14 mg/ml, MBCs = 0.28 mg/ml), and Trichoderma viride (MIC = 0.05 mg/ml, MFC = 0.11 mg/ml). Cytotoxic effect was determined by MTT test against malignant HeLa, LS174, and A549 cells (IC50 = 5.9 - 146.0 μg/ml), and against normal MRC-5 cells (IC50> 120.1 μg/ml). The best effect was exhibited by H. verticillatum root oil on A549 cells (IC50= 5.9 μg/ml), and H. ternatum root oil against LS174 cells (IC50= 6.7 μg/ml).
PB  - Wiley-VCH Verlag GMBH, Weinheim
T2  - Chemistry & Biodiversity
T1  - Chemical Composition, Antimicrobial and Cytotoxic Activity of Heracleum verticillatum Pančić and H. ternatum Velen. (Apiaceae) Essential Oils
VL  - 13
IS  - 4
SP  - 466
EP  - 476
DO  - 10.1002/cbdv.201500151
ER  - 

@article{
author = "Ušjak, Ljuboš and Petrović, Silvana and Drobac, Milica and Soković, Marina and Stanojković, Tatjana and Ćirić, Ana D. and Grozdanić, Nađa and Niketić, Marjan",
year = "2016",
abstract = "In this work, the chemical composition, antimicrobial and cytotoxic activity of Heracleum verticillatum Pančic and H. ternatum Velen. root, leaf, and fruit essential oils were investigated. The composition was analyzed by GC and GC/MS. Heracleum verticillatum and H. ternatum root oils were dominated by monoterpenes, mostly β-pinene (23.5% and 47.3%, respectively). Heracleum verticillatum leaf oil was characterized by monoterpenes, mainly limonene (20.3%), and sesquiterpenes, mostly (E)-caryophyllene (19.1%), while H. ternatum leaf oil by the high percentage of phenylpropanoids, with (Z)-isoelemicin (35.1%) being dominant constituent. Both fruit oils contained the majority of aliphatic esters, mostly octyl acetate (42.3% in H. verticillatum oil and 49.0% in H. ternatum oil). The antimicrobial activity of the oils was determined by microdilution method against eight bacterial and eight fungal strains. The strongest effect was exhibited by H. verticillatum root oil, particularly against Staphylococcus aureus, Salmonella typhimurium (MICs = 0.14 mg/ml, MBCs = 0.28 mg/ml), and Trichoderma viride (MIC = 0.05 mg/ml, MFC = 0.11 mg/ml). Cytotoxic effect was determined by MTT test against malignant HeLa, LS174, and A549 cells (IC50 = 5.9 - 146.0 μg/ml), and against normal MRC-5 cells (IC50> 120.1 μg/ml). The best effect was exhibited by H. verticillatum root oil on A549 cells (IC50= 5.9 μg/ml), and H. ternatum root oil against LS174 cells (IC50= 6.7 μg/ml).",
publisher = "Wiley-VCH Verlag GMBH, Weinheim",
journal = "Chemistry & Biodiversity",
title = "Chemical Composition, Antimicrobial and Cytotoxic Activity of Heracleum verticillatum Pančić and H. ternatum Velen. (Apiaceae) Essential Oils",
volume = "13",
number = "4",
pages = "466-476",
doi = "10.1002/cbdv.201500151"
}

Ušjak, L., Petrović, S., Drobac, M., Soković, M., Stanojković, T., Ćirić, A. D., Grozdanić, N.,& Niketić, M.. (2016). Chemical Composition, Antimicrobial and Cytotoxic Activity of Heracleum verticillatum Pančić and H. ternatum Velen. (Apiaceae) Essential Oils. in Chemistry & Biodiversity
Wiley-VCH Verlag GMBH, Weinheim., 13(4), 466-476.
https://doi.org/10.1002/cbdv.201500151

Ušjak L, Petrović S, Drobac M, Soković M, Stanojković T, Ćirić AD, Grozdanić N, Niketić M. Chemical Composition, Antimicrobial and Cytotoxic Activity of Heracleum verticillatum Pančić and H. ternatum Velen. (Apiaceae) Essential Oils. in Chemistry & Biodiversity. 2016;13(4):466-476.
doi:10.1002/cbdv.201500151 .

Ušjak, Ljuboš, Petrović, Silvana, Drobac, Milica, Soković, Marina, Stanojković, Tatjana, Ćirić, Ana D., Grozdanić, Nađa, Niketić, Marjan, "Chemical Composition, Antimicrobial and Cytotoxic Activity of Heracleum verticillatum Pančić and H. ternatum Velen. (Apiaceae) Essential Oils" in Chemistry & Biodiversity, 13, no. 4 (2016):466-476,
https://doi.org/10.1002/cbdv.201500151 . .

TY  - JOUR
AU  - Konić-Ristić, Aleksandra
AU  - Stanojković, Tatjana
AU  - Srdić-Rajić, Tatjana
AU  - Dilber, Sanda
AU  - Đorđević, Brižita
AU  - Stanković, Ivan
AU  - Juranić, Zorica
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2671
AB  - Background/Aim. Numerous epidemiological studies have shown beneficial effects of cruciferous vegetables consumption in cancer chemoprevention. Biologically active compounds of different Brassicaceae species with antitumor potential are isothiocyanates, present in the form of their precursors - glucosinolates. The aim of this study was to determine the selectivity of antiproliferative action of dietary isothiocyanates for malignant versus normal cells. Methods. Antiproliferative activity of three isothiocyanates abundant in human diet: sulforaphane, benzyl isothiocyanate (BITC) and phenylethyl isothiocyanate, on human cervix carcinoma cell line - HeLa, melanoma cell line - Fem-x, and colon cancer cell line - LS 174, and on peripheral blood mononuclear cells (PBMC), with or without mitogen, were determined by MTT colorimetric assay 72 h after their continuous action. Results. All investigated isothiocyanates inhibited the proliferation of HeLa, Fem-x and LS 174 cells. On all cell lines treated, BITC was the most potent inhibitor of cell proliferation with half-maximum inhibitory concentration (IC50) values of 5.04 mmoL m-3 on HeLa cells, 2.76 mmol m-3 on Fem-x, and 14.30 mmol m-3 on LS 174 cells. Antiproliferative effects on human PBMC were with higher IC50 than on malignant cells. Indexes of selectivity, calculated as a ratio between IC50 values obtained on PBMC and malignant cells, were between 1.12 and 16.57, with the highest values obtained for the action of BITC on melanoma Fem-x cells. Conclusion. Based on its antiproliferative effects on malignant cells, as well as the selectivity of the action to malignant vs normal cells, benzyl isothiocyanate can be considered as a promising candidate in cancer chemoprevention. In general, the safety of investigated compounds, in addition to their antitumor potential, should be considered as an important criterion in cancer chemoprevention. Screening of selectivity is a plausible approach to the evaluation of safety of both natural isothiocyanates and synthesised analogues of these bioactive compounds.
AB  - Uvod/Cilj. Brojne epidemiološke studije pokazale su povoljne efekte konzumiranja povrća iz familije kupusnjača (Brassicaceae) u hemioprevenciji karcinoma. Osnovni biološki aktivni sastojci ovog povrća su izotiocijanati, prisutni u obliku prekursora - glukozinolata. Cilj ovog rada bio je određivanje selektivnosti antiproliferativnog delovanja dijetetskih izotiocijanata na maligne ćelije u odnosu na normalne ćelije. Metode. Antiproliferativna aktivnost tri izotiocijanata zastupljena u ljudskoj ishrani: sulforafana (SFN), benzil-izotiocijanata (BITC) i feniletil-izotiocijanata (FEITC) na humane maligne ćelijske linije, HeLa, ćelijsku liniju karcinoma grlića materice, Fem-x, ćelijsku liniju melanoma, i LS 174, ćelijsku liniju karcinoma kolona, kao i na mononuklearne ćelije periferne krvi (MNĆPK), sa ili bez delovanja mitogena, određivana je MTT kalorimetrijskim testom, 72 h nakon kontinuiranog delovanja agenasa. Rezultati. Svi ispitivani izotiocijanati inhibirali su proliferaciju HeLa, Fem-x i LS 174 ćelija. Na svim ćelijskim linijama BITC je pokazao najizraženije delovanje sa vrednostima polumaksimalne inhibitorne koncentracije (IC50) od 5.04 mmol m-3 na HeLa ćelijama, 2,76 mmoL m-3 na Fem-x i 14,30 mmol m-3 na LS 174 ćelijama. Svi ispitivani izotiocijanati pokazali su citotoksično delovanje na MNĆPK, ali sa višim IC50 vrednostima u odnosu na maligne ćelije. Indeksi selektivnosti antitumorkog delovanja, izraženi kao odnos IC50 vrednosti dobijenih na MNĆPK i malignim ćelijama, bili su između 1,12 i 16,57, sa najvišom vrednosti pri delovanju BITC na Fem-x ćelije. Zaključak. Na osnovu antiproliferativne aktivnosti na maligne ćelije i selektivnosti antiproliferativnog delovanja na maligne u odnosu na normalne ćelije, benzil izotiocijanat se ističe kao perspektivni agens u hemioprevenciji karcinoma. Generalno, pored antitumorskog delovanja, bezbednost primene ovih jedinjenja treba da predstavlja važan kriterijum u izboru odgovarajućih izotiocijanata za primenu u primarnoj, sekundarnoj i tercijarnoj hemioprevenciji kancera. Ispitivanje selektivnosti predstavlja pogodan pristup oceni bezbednosti i prirodnih izotiocijanata i sintetskih analoga.
PB  - Vojnomedicinska akademija - Institut za naučne informacije, Beograd
T2  - Vojnosanitetski pregled
T1  - In vitro assessment of antiproliferative action selectivity of dietary isothiocyanates for tumor versus normal human cells
T1  - In vitro ispitivanje selektivnosti antiproliferativnog dejstva dijetetskih izotiocijanata na tumorske u odnosu na normalne humane ćelije
VL  - 73
IS  - 7
SP  - 636
EP  - 642
DO  - 10.2298/VSP141103066K
ER  - 

@article{
author = "Konić-Ristić, Aleksandra and Stanojković, Tatjana and Srdić-Rajić, Tatjana and Dilber, Sanda and Đorđević, Brižita and Stanković, Ivan and Juranić, Zorica",
year = "2016",
abstract = "Background/Aim. Numerous epidemiological studies have shown beneficial effects of cruciferous vegetables consumption in cancer chemoprevention. Biologically active compounds of different Brassicaceae species with antitumor potential are isothiocyanates, present in the form of their precursors - glucosinolates. The aim of this study was to determine the selectivity of antiproliferative action of dietary isothiocyanates for malignant versus normal cells. Methods. Antiproliferative activity of three isothiocyanates abundant in human diet: sulforaphane, benzyl isothiocyanate (BITC) and phenylethyl isothiocyanate, on human cervix carcinoma cell line - HeLa, melanoma cell line - Fem-x, and colon cancer cell line - LS 174, and on peripheral blood mononuclear cells (PBMC), with or without mitogen, were determined by MTT colorimetric assay 72 h after their continuous action. Results. All investigated isothiocyanates inhibited the proliferation of HeLa, Fem-x and LS 174 cells. On all cell lines treated, BITC was the most potent inhibitor of cell proliferation with half-maximum inhibitory concentration (IC50) values of 5.04 mmoL m-3 on HeLa cells, 2.76 mmol m-3 on Fem-x, and 14.30 mmol m-3 on LS 174 cells. Antiproliferative effects on human PBMC were with higher IC50 than on malignant cells. Indexes of selectivity, calculated as a ratio between IC50 values obtained on PBMC and malignant cells, were between 1.12 and 16.57, with the highest values obtained for the action of BITC on melanoma Fem-x cells. Conclusion. Based on its antiproliferative effects on malignant cells, as well as the selectivity of the action to malignant vs normal cells, benzyl isothiocyanate can be considered as a promising candidate in cancer chemoprevention. In general, the safety of investigated compounds, in addition to their antitumor potential, should be considered as an important criterion in cancer chemoprevention. Screening of selectivity is a plausible approach to the evaluation of safety of both natural isothiocyanates and synthesised analogues of these bioactive compounds., Uvod/Cilj. Brojne epidemiološke studije pokazale su povoljne efekte konzumiranja povrća iz familije kupusnjača (Brassicaceae) u hemioprevenciji karcinoma. Osnovni biološki aktivni sastojci ovog povrća su izotiocijanati, prisutni u obliku prekursora - glukozinolata. Cilj ovog rada bio je određivanje selektivnosti antiproliferativnog delovanja dijetetskih izotiocijanata na maligne ćelije u odnosu na normalne ćelije. Metode. Antiproliferativna aktivnost tri izotiocijanata zastupljena u ljudskoj ishrani: sulforafana (SFN), benzil-izotiocijanata (BITC) i feniletil-izotiocijanata (FEITC) na humane maligne ćelijske linije, HeLa, ćelijsku liniju karcinoma grlića materice, Fem-x, ćelijsku liniju melanoma, i LS 174, ćelijsku liniju karcinoma kolona, kao i na mononuklearne ćelije periferne krvi (MNĆPK), sa ili bez delovanja mitogena, određivana je MTT kalorimetrijskim testom, 72 h nakon kontinuiranog delovanja agenasa. Rezultati. Svi ispitivani izotiocijanati inhibirali su proliferaciju HeLa, Fem-x i LS 174 ćelija. Na svim ćelijskim linijama BITC je pokazao najizraženije delovanje sa vrednostima polumaksimalne inhibitorne koncentracije (IC50) od 5.04 mmol m-3 na HeLa ćelijama, 2,76 mmoL m-3 na Fem-x i 14,30 mmol m-3 na LS 174 ćelijama. Svi ispitivani izotiocijanati pokazali su citotoksično delovanje na MNĆPK, ali sa višim IC50 vrednostima u odnosu na maligne ćelije. Indeksi selektivnosti antitumorkog delovanja, izraženi kao odnos IC50 vrednosti dobijenih na MNĆPK i malignim ćelijama, bili su između 1,12 i 16,57, sa najvišom vrednosti pri delovanju BITC na Fem-x ćelije. Zaključak. Na osnovu antiproliferativne aktivnosti na maligne ćelije i selektivnosti antiproliferativnog delovanja na maligne u odnosu na normalne ćelije, benzil izotiocijanat se ističe kao perspektivni agens u hemioprevenciji karcinoma. Generalno, pored antitumorskog delovanja, bezbednost primene ovih jedinjenja treba da predstavlja važan kriterijum u izboru odgovarajućih izotiocijanata za primenu u primarnoj, sekundarnoj i tercijarnoj hemioprevenciji kancera. Ispitivanje selektivnosti predstavlja pogodan pristup oceni bezbednosti i prirodnih izotiocijanata i sintetskih analoga.",
publisher = "Vojnomedicinska akademija - Institut za naučne informacije, Beograd",
journal = "Vojnosanitetski pregled",
title = "In vitro assessment of antiproliferative action selectivity of dietary isothiocyanates for tumor versus normal human cells, In vitro ispitivanje selektivnosti antiproliferativnog dejstva dijetetskih izotiocijanata na tumorske u odnosu na normalne humane ćelije",
volume = "73",
number = "7",
pages = "636-642",
doi = "10.2298/VSP141103066K"
}

Konić-Ristić, A., Stanojković, T., Srdić-Rajić, T., Dilber, S., Đorđević, B., Stanković, I.,& Juranić, Z.. (2016). In vitro assessment of antiproliferative action selectivity of dietary isothiocyanates for tumor versus normal human cells. in Vojnosanitetski pregled
Vojnomedicinska akademija - Institut za naučne informacije, Beograd., 73(7), 636-642.
https://doi.org/10.2298/VSP141103066K

Konić-Ristić A, Stanojković T, Srdić-Rajić T, Dilber S, Đorđević B, Stanković I, Juranić Z. In vitro assessment of antiproliferative action selectivity of dietary isothiocyanates for tumor versus normal human cells. in Vojnosanitetski pregled. 2016;73(7):636-642.
doi:10.2298/VSP141103066K .

Konić-Ristić, Aleksandra, Stanojković, Tatjana, Srdić-Rajić, Tatjana, Dilber, Sanda, Đorđević, Brižita, Stanković, Ivan, Juranić, Zorica, "In vitro assessment of antiproliferative action selectivity of dietary isothiocyanates for tumor versus normal human cells" in Vojnosanitetski pregled, 73, no. 7 (2016):636-642,
https://doi.org/10.2298/VSP141103066K . .

TY  - JOUR
AU  - Damjanović, Ana B.
AU  - Matić, Ivana Z.
AU  - Dordić, Marija
AU  - Nikolić-Durović, Marina
AU  - Nikolić, Srdan
AU  - Roki, Ksenija
AU  - Milovanović, Zorka
AU  - Antić-Stanković, Jelena
AU  - Dzodić, Radan
AU  - Damjanović, Svetozar S.
AU  - Kanjer, Ksenija
AU  - Abu Rabi, Zaki
AU  - Juranić, Zorica
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2457
AB  - The aim of research was to determine the effects of maximally therapeutically achievable concentrations of metformin on malignant cells and healthy peripheral blood mononuclear cells (PBMC). Eight patients with T2D or hyperglycemia and nine healthy volunteers were included in the study. For determination of the influence of metformin on the phenotype of breast carcinoma, 1,410 patients with surgically removed tumors were included. From this group 37 breast cancer patients had DM type 2 or hyperglycemia and were pretreated with metformin alone or sometimes in combination with other antidiabetic drugs. Our results proved that metformin at low concentrations induced mild decrease in survival of malignant cells and PBMC stimulated for proliferation, but it didn't affect survival of resting PBMC. The effects of plasma of hyperglycemic patients who were under metformin therapy on autologous PBMC-induced decrease in survival of MDA-MB-361 cells, was noticeable in some patients. Metformin pretreatment for 24 h of HER2+ MDA-MB-361 cells, which were subsequently treated for 48 h with Herceptin, induced additional decline in cell survival. The analysis of influence of metformin on phenotype of breast cancer cells revealed significantly lower number of diabetic cancer patients treated with metformin with overexpressed HER2+ tumors (p  lt  0.013), while the number of patients with ER+PR+ tumors was not significantly changed (p  lt  0.832). In conclusion, therapeutically used concentrations of metformin exhibit mild cytotoxic action on malignant and dividing normal cells pointing to its preferred role in malignant and autoimmune diseases. The use of metformin was associated with pronounced decrease in HER2 overexpressing tumors.
PB  - Springer, Dordrecht
T2  - Pathology & Oncology Research
T1  - Metformin Effects on Malignant Cells and Healthy PBMC; The Influence of Metformin on the Phenotype of Breast Cancer Cells
VL  - 21
IS  - 3
SP  - 605
EP  - 612
DO  - 10.1007/s12253-014-9864-9
ER  - 

@article{
author = "Damjanović, Ana B. and Matić, Ivana Z. and Dordić, Marija and Nikolić-Durović, Marina and Nikolić, Srdan and Roki, Ksenija and Milovanović, Zorka and Antić-Stanković, Jelena and Dzodić, Radan and Damjanović, Svetozar S. and Kanjer, Ksenija and Abu Rabi, Zaki and Juranić, Zorica",
year = "2015",
abstract = "The aim of research was to determine the effects of maximally therapeutically achievable concentrations of metformin on malignant cells and healthy peripheral blood mononuclear cells (PBMC). Eight patients with T2D or hyperglycemia and nine healthy volunteers were included in the study. For determination of the influence of metformin on the phenotype of breast carcinoma, 1,410 patients with surgically removed tumors were included. From this group 37 breast cancer patients had DM type 2 or hyperglycemia and were pretreated with metformin alone or sometimes in combination with other antidiabetic drugs. Our results proved that metformin at low concentrations induced mild decrease in survival of malignant cells and PBMC stimulated for proliferation, but it didn't affect survival of resting PBMC. The effects of plasma of hyperglycemic patients who were under metformin therapy on autologous PBMC-induced decrease in survival of MDA-MB-361 cells, was noticeable in some patients. Metformin pretreatment for 24 h of HER2+ MDA-MB-361 cells, which were subsequently treated for 48 h with Herceptin, induced additional decline in cell survival. The analysis of influence of metformin on phenotype of breast cancer cells revealed significantly lower number of diabetic cancer patients treated with metformin with overexpressed HER2+ tumors (p  lt  0.013), while the number of patients with ER+PR+ tumors was not significantly changed (p  lt  0.832). In conclusion, therapeutically used concentrations of metformin exhibit mild cytotoxic action on malignant and dividing normal cells pointing to its preferred role in malignant and autoimmune diseases. The use of metformin was associated with pronounced decrease in HER2 overexpressing tumors.",
publisher = "Springer, Dordrecht",
journal = "Pathology & Oncology Research",
title = "Metformin Effects on Malignant Cells and Healthy PBMC; The Influence of Metformin on the Phenotype of Breast Cancer Cells",
volume = "21",
number = "3",
pages = "605-612",
doi = "10.1007/s12253-014-9864-9"
}

Damjanović, A. B., Matić, I. Z., Dordić, M., Nikolić-Durović, M., Nikolić, S., Roki, K., Milovanović, Z., Antić-Stanković, J., Dzodić, R., Damjanović, S. S., Kanjer, K., Abu Rabi, Z.,& Juranić, Z.. (2015). Metformin Effects on Malignant Cells and Healthy PBMC; The Influence of Metformin on the Phenotype of Breast Cancer Cells. in Pathology & Oncology Research
Springer, Dordrecht., 21(3), 605-612.
https://doi.org/10.1007/s12253-014-9864-9

Damjanović AB, Matić IZ, Dordić M, Nikolić-Durović M, Nikolić S, Roki K, Milovanović Z, Antić-Stanković J, Dzodić R, Damjanović SS, Kanjer K, Abu Rabi Z, Juranić Z. Metformin Effects on Malignant Cells and Healthy PBMC; The Influence of Metformin on the Phenotype of Breast Cancer Cells. in Pathology & Oncology Research. 2015;21(3):605-612.
doi:10.1007/s12253-014-9864-9 .

Damjanović, Ana B., Matić, Ivana Z., Dordić, Marija, Nikolić-Durović, Marina, Nikolić, Srdan, Roki, Ksenija, Milovanović, Zorka, Antić-Stanković, Jelena, Dzodić, Radan, Damjanović, Svetozar S., Kanjer, Ksenija, Abu Rabi, Zaki, Juranić, Zorica, "Metformin Effects on Malignant Cells and Healthy PBMC; The Influence of Metformin on the Phenotype of Breast Cancer Cells" in Pathology & Oncology Research, 21, no. 3 (2015):605-612,
https://doi.org/10.1007/s12253-014-9864-9 . .

TY  - JOUR
AU  - Tanasković, Slađana
AU  - Antonijević-Nikolić, Mirjana
AU  - Hollo, Berta Barta
AU  - Dražić, Branka
AU  - Stanojković, Tatjana
AU  - Meszaros-Szecsenyi, Katalin
AU  - Vucković, Gordana
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2131
AB  - Seven macrocyclic dinuclear Cu(II) complexes with tpmc = = N,N ',N '',N'''-tetrakis(2-pyridylmethyl)-1,4,8,11-tetraazacyclotetradecane of coordination formulae [Cu(2)tpmc](ClO4)(4) (1), [Cu-2(X)tpmc](ClO4)(3)center dot nH(2)O, X= F-, n = 0 (2), X = Cl-, n = 1 (3), X = Br-, n = 0 (4), X= I-, n = 1 (5), X = = NO2-, n = 0 (6), [Cu-2(NCS)(2)tpmc](ClO4)(2) (7) were evaluated for their cytotoxic activity against human cervix adenocarcinoma (HeLa), human melanoma (Fem-x) and human colon carcinoma (LS174) cell lines. The results were compared with the corresponding data for the cis-diamminedichloridoplafinum(H) (CDDP) as referent cytostatic, as well as with the free ligands and the solvent dimethyl sulfoxide (DMSO) as controls. The complexes showed considerable antiproliferative effect, although significantly less than CDDP. The thermal decomposition pattern of the complexes was determined by simultaneous TG/DSC measurements. The thermal stability of the compounds 2-7 followed the trend of their antiproliferative activity against the HeLa cell line, as well as their corresponding stability constants. The highest thermal stability and cytotoxicity belonged to complex [Cu(2)tpmc](ClO4)(4), with no anionic co-ligand. Complex [Cu-2(NO2)tpmc](ClO4)(3) exhibited a selective cytotoxicity against LS174 cells, at the level of the most active [Cu(2)tpmc](ClO4)(4).
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Correlations between the in vitro antiproliferative activity, structure and thermal stability of some macrocyclic dinuclear Cu(II) complexes
VL  - 79
IS  - 10
SP  - 1235
EP  - 1247
DO  - 10.2298/JSC140404044T
ER  - 

@article{
author = "Tanasković, Slađana and Antonijević-Nikolić, Mirjana and Hollo, Berta Barta and Dražić, Branka and Stanojković, Tatjana and Meszaros-Szecsenyi, Katalin and Vucković, Gordana",
year = "2014",
abstract = "Seven macrocyclic dinuclear Cu(II) complexes with tpmc = = N,N ',N '',N'''-tetrakis(2-pyridylmethyl)-1,4,8,11-tetraazacyclotetradecane of coordination formulae [Cu(2)tpmc](ClO4)(4) (1), [Cu-2(X)tpmc](ClO4)(3)center dot nH(2)O, X= F-, n = 0 (2), X = Cl-, n = 1 (3), X = Br-, n = 0 (4), X= I-, n = 1 (5), X = = NO2-, n = 0 (6), [Cu-2(NCS)(2)tpmc](ClO4)(2) (7) were evaluated for their cytotoxic activity against human cervix adenocarcinoma (HeLa), human melanoma (Fem-x) and human colon carcinoma (LS174) cell lines. The results were compared with the corresponding data for the cis-diamminedichloridoplafinum(H) (CDDP) as referent cytostatic, as well as with the free ligands and the solvent dimethyl sulfoxide (DMSO) as controls. The complexes showed considerable antiproliferative effect, although significantly less than CDDP. The thermal decomposition pattern of the complexes was determined by simultaneous TG/DSC measurements. The thermal stability of the compounds 2-7 followed the trend of their antiproliferative activity against the HeLa cell line, as well as their corresponding stability constants. The highest thermal stability and cytotoxicity belonged to complex [Cu(2)tpmc](ClO4)(4), with no anionic co-ligand. Complex [Cu-2(NO2)tpmc](ClO4)(3) exhibited a selective cytotoxicity against LS174 cells, at the level of the most active [Cu(2)tpmc](ClO4)(4).",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Correlations between the in vitro antiproliferative activity, structure and thermal stability of some macrocyclic dinuclear Cu(II) complexes",
volume = "79",
number = "10",
pages = "1235-1247",
doi = "10.2298/JSC140404044T"
}

Tanasković, S., Antonijević-Nikolić, M., Hollo, B. B., Dražić, B., Stanojković, T., Meszaros-Szecsenyi, K.,& Vucković, G.. (2014). Correlations between the in vitro antiproliferative activity, structure and thermal stability of some macrocyclic dinuclear Cu(II) complexes. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 79(10), 1235-1247.
https://doi.org/10.2298/JSC140404044T

Tanasković S, Antonijević-Nikolić M, Hollo BB, Dražić B, Stanojković T, Meszaros-Szecsenyi K, Vucković G. Correlations between the in vitro antiproliferative activity, structure and thermal stability of some macrocyclic dinuclear Cu(II) complexes. in Journal of the Serbian Chemical Society. 2014;79(10):1235-1247.
doi:10.2298/JSC140404044T .

Tanasković, Slađana, Antonijević-Nikolić, Mirjana, Hollo, Berta Barta, Dražić, Branka, Stanojković, Tatjana, Meszaros-Szecsenyi, Katalin, Vucković, Gordana, "Correlations between the in vitro antiproliferative activity, structure and thermal stability of some macrocyclic dinuclear Cu(II) complexes" in Journal of the Serbian Chemical Society, 79, no. 10 (2014):1235-1247,
https://doi.org/10.2298/JSC140404044T . .

TY  - JOUR
AU  - Kundaković, Tatjana
AU  - Stanojković, Tatjana
AU  - Kolundžija, Branka
AU  - Marković, Stevan
AU  - Šukilović, Branka
AU  - Milenković, Marina
AU  - Lakušić, Branislava
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2217
AB  - The antimicrobial and cytotoxic activities of the essential oil of Satureja montana ssp. pisidica from two localities (mountains Korab and Galicica) were studied. Forty-nine components were identified in the each sample. Oxygenated monoterpene hydrocarbons were the major compounds: carvacrol, thymol, carvacrol methyl ether and beta-linalool. Both tested essential oils showed very high and similar antimicrobial activity. Minimal inhibitory concentrations ranged from 12.5 mu g/mL against S. epidermidis to 50 mu g/mL against P. aerugmosa and C. albicans. The cytotoxic effect of the essential oils was tested against MDA-MB-361, MDA-MB-453, HeLa, LS174 and MRCS cells. The essential oil from Korab demonstrated significantly better results than the oil from Galicica, particularly against HeLa and MDA-MB-453 cell lines, with IC50 values of 63.5 and 72.3 mu g/mL, while the oil from Galicica was the most active on the human epithelial cervical cancer HeLa cells (IC50 99.7 mu g/mL).
PB  - Sage Publications Inc, Thousand Oaks
T2  - Natural Product Communications
T1  - Cytotoxicity and Antimicrobial Activity of the Essential Oil from Satureja montana subsp pisidica (Lamiceae)
VL  - 9
IS  - 4
SP  - 569
EP  - 572
UR  - https://hdl.handle.net/21.15107/rcub_farfar_2217
ER  - 

@article{
author = "Kundaković, Tatjana and Stanojković, Tatjana and Kolundžija, Branka and Marković, Stevan and Šukilović, Branka and Milenković, Marina and Lakušić, Branislava",
year = "2014",
abstract = "The antimicrobial and cytotoxic activities of the essential oil of Satureja montana ssp. pisidica from two localities (mountains Korab and Galicica) were studied. Forty-nine components were identified in the each sample. Oxygenated monoterpene hydrocarbons were the major compounds: carvacrol, thymol, carvacrol methyl ether and beta-linalool. Both tested essential oils showed very high and similar antimicrobial activity. Minimal inhibitory concentrations ranged from 12.5 mu g/mL against S. epidermidis to 50 mu g/mL against P. aerugmosa and C. albicans. The cytotoxic effect of the essential oils was tested against MDA-MB-361, MDA-MB-453, HeLa, LS174 and MRCS cells. The essential oil from Korab demonstrated significantly better results than the oil from Galicica, particularly against HeLa and MDA-MB-453 cell lines, with IC50 values of 63.5 and 72.3 mu g/mL, while the oil from Galicica was the most active on the human epithelial cervical cancer HeLa cells (IC50 99.7 mu g/mL).",
publisher = "Sage Publications Inc, Thousand Oaks",
journal = "Natural Product Communications",
title = "Cytotoxicity and Antimicrobial Activity of the Essential Oil from Satureja montana subsp pisidica (Lamiceae)",
volume = "9",
number = "4",
pages = "569-572",
url = "https://hdl.handle.net/21.15107/rcub_farfar_2217"
}

Kundaković, T., Stanojković, T., Kolundžija, B., Marković, S., Šukilović, B., Milenković, M.,& Lakušić, B.. (2014). Cytotoxicity and Antimicrobial Activity of the Essential Oil from Satureja montana subsp pisidica (Lamiceae). in Natural Product Communications
Sage Publications Inc, Thousand Oaks., 9(4), 569-572.
https://hdl.handle.net/21.15107/rcub_farfar_2217

Kundaković T, Stanojković T, Kolundžija B, Marković S, Šukilović B, Milenković M, Lakušić B. Cytotoxicity and Antimicrobial Activity of the Essential Oil from Satureja montana subsp pisidica (Lamiceae). in Natural Product Communications. 2014;9(4):569-572.
https://hdl.handle.net/21.15107/rcub_farfar_2217 .

Kundaković, Tatjana, Stanojković, Tatjana, Kolundžija, Branka, Marković, Stevan, Šukilović, Branka, Milenković, Marina, Lakušić, Branislava, "Cytotoxicity and Antimicrobial Activity of the Essential Oil from Satureja montana subsp pisidica (Lamiceae)" in Natural Product Communications, 9, no. 4 (2014):569-572,
https://hdl.handle.net/21.15107/rcub_farfar_2217 .

TY  - JOUR
AU  - Veinović, Gorana
AU  - Filipić, Brankica
AU  - Stanković, Jelena
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2067
AB  - Lyme borreliosis is the most common vector-borne disease in the northern hemisphere. The agents of Lyme borreliosis are borrelia, bacteria of the family Spirochaetaceae, which are grouped in Borrelia burgdorferi sensu lato species complex. Borreliae are fastidious, slow-growing and biochemically inactive bacteria that need special attention and optimal conditions for cultivation. The isolation of Borrelia from clinical material and their cultivation is a time-consuming and demanding procedure. Cultivation lasts from 9 up to 12 weeks, which is much longer than is necessary to grow most other human bacterial pathogens. Although B. burgdorferi sensu lato is susceptible to a wide range of antimicrobial agents in vitro, up to now the susceptibility of individual Borrelia species to antibiotics is defined only partially.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Isolation, cultivation, and in vitro susceptibility testing of Borrelia burgdorferi sensu lato: A review
VL  - 65
IS  - 2
SP  - 533
EP  - 547
DO  - 10.2298/ABS1302533V
ER  - 

@article{
author = "Veinović, Gorana and Filipić, Brankica and Stanković, Jelena",
year = "2013",
abstract = "Lyme borreliosis is the most common vector-borne disease in the northern hemisphere. The agents of Lyme borreliosis are borrelia, bacteria of the family Spirochaetaceae, which are grouped in Borrelia burgdorferi sensu lato species complex. Borreliae are fastidious, slow-growing and biochemically inactive bacteria that need special attention and optimal conditions for cultivation. The isolation of Borrelia from clinical material and their cultivation is a time-consuming and demanding procedure. Cultivation lasts from 9 up to 12 weeks, which is much longer than is necessary to grow most other human bacterial pathogens. Although B. burgdorferi sensu lato is susceptible to a wide range of antimicrobial agents in vitro, up to now the susceptibility of individual Borrelia species to antibiotics is defined only partially.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Isolation, cultivation, and in vitro susceptibility testing of Borrelia burgdorferi sensu lato: A review",
volume = "65",
number = "2",
pages = "533-547",
doi = "10.2298/ABS1302533V"
}

Veinović, G., Filipić, B.,& Stanković, J.. (2013). Isolation, cultivation, and in vitro susceptibility testing of Borrelia burgdorferi sensu lato: A review. in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 65(2), 533-547.
https://doi.org/10.2298/ABS1302533V

Veinović G, Filipić B, Stanković J. Isolation, cultivation, and in vitro susceptibility testing of Borrelia burgdorferi sensu lato: A review. in Archives of Biological Sciences. 2013;65(2):533-547.
doi:10.2298/ABS1302533V .

Veinović, Gorana, Filipić, Brankica, Stanković, Jelena, "Isolation, cultivation, and in vitro susceptibility testing of Borrelia burgdorferi sensu lato: A review" in Archives of Biological Sciences, 65, no. 2 (2013):533-547,
https://doi.org/10.2298/ABS1302533V . .

TY  - JOUR
AU  - Tanasković, Slađana
AU  - Antić-Stanković, Jelena
AU  - Antonijević-Nikolić, Mirjana
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2039
AB  - Four cationic mixed-ligand complexes of Cu (II) with N, N', N'', N''' -tetrakis (2-pyridylmethyl) -1,4,8,11 -tetraazacyclotetradecane (tpmc) and aliphatic dicarboxylic acids: succinic, glutaric , adipic and sebacic were investigated in vitro on the human cervix adenocarcinoma (HeLa), human malignant melanoma (Fem-x), oestrogen-receptor-positive human breast cancer (MCF-7) cells and the human acute monocyte leukemia cell line (THP1), while free ligands, Cu(II) salt and solvent (used as a controls) were inactive on the same conditions. The metabolic activity of the cells was determined by tetrazolium MTS test. All complexes showed cytotoxicity toward the used cell lines, while free ligands, Cu(II) salt and solvent (used as a controls) were inactive on the same conditions. Increasing the number of - CH2- groups in homologous serie of aliphatic dicarboxylate ligands leads to the increasing cytotoxic activity of the complex. The highest cytotoxic effect of azamacrocycle Cu(II) complexes with glutarate, adipate, sebacinate ligand showed on the cell line MCF-7 and the worst on HeLa cells. A significant effect of sebacinate Cu(II)complex to all tested tumor cell lines applied in concentrations of 100 and 200 μM and up to HeLa cells.
AB  - Četiri katjonska mešovito-ligandna kompleksa Cu(II) sa N,N',N'N''-tetrakis(2- piridilmetil)-1,4,8,11-tetraaza-ciklotetradekanom i alifatičnim dikarboksilatima: ćilibarnom, glutarnom, adipinskom i sebacinskom kiselinom su ispitivani na humanim malignim ćelijskim linijama: cervikalnog adenokarcinoma (HeLa), melanoma (Fem-X), estrogen pozitivnog karcinoma dojke (MCF-7) i akutne monocitne leukemjie (THP1). Uporedo su testirani i sami ligandi, proste soli i rastvarači. Metabolička aktivnost ćelija je određena MTS testom. Svi ispitivani kompleksi su pokazali citotoksični efekat prema testiranim ćelijskim linijama, dok su slobodni ligandi, Cu(II) so i rastvarač (koji su korišćeni kao kontrolni) pod istim uslovima, bili neaktivni. Sa povećanjem broja -CH2- grupa homologog niza alifatičnih dikarboksilato liganada dolazi i do povećane citotoksičnosti kompleksa. Najveći citotoksični efekat su pokazali makrociklični Cu(II) kompleksi sa glutarato, adipato i sebacinato ligandima prema MCF-7, a najslabiji prema HeLa ćelijama. Najveći antiproliferativni efekat pokazuje kompleks sa sebacinato ligandom prema svim testiranim linijama tumorskih ćelija u primenjenim koncentracijama od 100 i 200 μM, a najviše prema HeLa ćelijama.
PB  - Savez farmaceutskih udruženja Srbije, Beograd
T2  - Arhiv za farmaciju
T1  - Antiproliferative activity of mixed-ligand Cu(II) complexes with pendant octaazamacrocycle in vitro
T1  - Antiproliferativna aktivnost mešovito-ligandnih Cu (II) kompleksa sa pendantnim oktaazamakrociklom in vitro
VL  - 63
IS  - 4
SP  - 365
EP  - 374
UR  - https://hdl.handle.net/21.15107/rcub_farfar_2039
ER  - 

@article{
author = "Tanasković, Slađana and Antić-Stanković, Jelena and Antonijević-Nikolić, Mirjana",
year = "2013",
abstract = "Four cationic mixed-ligand complexes of Cu (II) with N, N', N'', N''' -tetrakis (2-pyridylmethyl) -1,4,8,11 -tetraazacyclotetradecane (tpmc) and aliphatic dicarboxylic acids: succinic, glutaric , adipic and sebacic were investigated in vitro on the human cervix adenocarcinoma (HeLa), human malignant melanoma (Fem-x), oestrogen-receptor-positive human breast cancer (MCF-7) cells and the human acute monocyte leukemia cell line (THP1), while free ligands, Cu(II) salt and solvent (used as a controls) were inactive on the same conditions. The metabolic activity of the cells was determined by tetrazolium MTS test. All complexes showed cytotoxicity toward the used cell lines, while free ligands, Cu(II) salt and solvent (used as a controls) were inactive on the same conditions. Increasing the number of - CH2- groups in homologous serie of aliphatic dicarboxylate ligands leads to the increasing cytotoxic activity of the complex. The highest cytotoxic effect of azamacrocycle Cu(II) complexes with glutarate, adipate, sebacinate ligand showed on the cell line MCF-7 and the worst on HeLa cells. A significant effect of sebacinate Cu(II)complex to all tested tumor cell lines applied in concentrations of 100 and 200 μM and up to HeLa cells., Četiri katjonska mešovito-ligandna kompleksa Cu(II) sa N,N',N'N''-tetrakis(2- piridilmetil)-1,4,8,11-tetraaza-ciklotetradekanom i alifatičnim dikarboksilatima: ćilibarnom, glutarnom, adipinskom i sebacinskom kiselinom su ispitivani na humanim malignim ćelijskim linijama: cervikalnog adenokarcinoma (HeLa), melanoma (Fem-X), estrogen pozitivnog karcinoma dojke (MCF-7) i akutne monocitne leukemjie (THP1). Uporedo su testirani i sami ligandi, proste soli i rastvarači. Metabolička aktivnost ćelija je određena MTS testom. Svi ispitivani kompleksi su pokazali citotoksični efekat prema testiranim ćelijskim linijama, dok su slobodni ligandi, Cu(II) so i rastvarač (koji su korišćeni kao kontrolni) pod istim uslovima, bili neaktivni. Sa povećanjem broja -CH2- grupa homologog niza alifatičnih dikarboksilato liganada dolazi i do povećane citotoksičnosti kompleksa. Najveći citotoksični efekat su pokazali makrociklični Cu(II) kompleksi sa glutarato, adipato i sebacinato ligandima prema MCF-7, a najslabiji prema HeLa ćelijama. Najveći antiproliferativni efekat pokazuje kompleks sa sebacinato ligandom prema svim testiranim linijama tumorskih ćelija u primenjenim koncentracijama od 100 i 200 μM, a najviše prema HeLa ćelijama.",
publisher = "Savez farmaceutskih udruženja Srbije, Beograd",
journal = "Arhiv za farmaciju",
title = "Antiproliferative activity of mixed-ligand Cu(II) complexes with pendant octaazamacrocycle in vitro, Antiproliferativna aktivnost mešovito-ligandnih Cu (II) kompleksa sa pendantnim oktaazamakrociklom in vitro",
volume = "63",
number = "4",
pages = "365-374",
url = "https://hdl.handle.net/21.15107/rcub_farfar_2039"
}

Tanasković, S., Antić-Stanković, J.,& Antonijević-Nikolić, M.. (2013). Antiproliferative activity of mixed-ligand Cu(II) complexes with pendant octaazamacrocycle in vitro. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije, Beograd., 63(4), 365-374.
https://hdl.handle.net/21.15107/rcub_farfar_2039

Tanasković S, Antić-Stanković J, Antonijević-Nikolić M. Antiproliferative activity of mixed-ligand Cu(II) complexes with pendant octaazamacrocycle in vitro. in Arhiv za farmaciju. 2013;63(4):365-374.
https://hdl.handle.net/21.15107/rcub_farfar_2039 .

Tanasković, Slađana, Antić-Stanković, Jelena, Antonijević-Nikolić, Mirjana, "Antiproliferative activity of mixed-ligand Cu(II) complexes with pendant octaazamacrocycle in vitro" in Arhiv za farmaciju, 63, no. 4 (2013):365-374,
https://hdl.handle.net/21.15107/rcub_farfar_2039 .