TY  - JOUR
AU  - Žugić, Ana
AU  - Isaković, Aleksandra
AU  - Jeremić, Ivica
AU  - Savić, Snežana
AU  - Tadić, Vanja
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4664
AB  - Old man’s beard (Usnea barbata) is one of the most investigated lichens which biological properties
(such as antimicrobial, anti-inflammatory and cytotoxic activities) have been proven in several scien-
tific studies. Stated properties of old man’s beard have been attributed mainly to its major secondary
metabolite - a dibenzofuran derivative, usnic acid. The aim of the current study was to examine cyto-
toxic activity of the quantified supercritical CO2 extract and pure usnic acid in parallel against L929
fibrosarcoma cells. Our results indicated the tested extract to be more toxic towards the investigated
cell line compared to usnic acid per se, despite the high amount of this compound (81.41% (w/w))
revealed in the extract using HPLC. Such result may be connected to the unidentified compounds
present in the supercritical CO2 extract that could be responsible for apoptosis and oxidative stress in
the exerted cytotoxicity of the investigated extract, but not usnic acid. Our study supports further in-
vestigations of supercritical CO2 extract of U. barbata as a prospective therapeutic agent with potential
relevance in the treatment of sarcoma.
PB  - Institut za proučavanje lekovitog bilja "Dr Josif Pančić", Beograd
T2  - Lekovite sirovine
T1  - Cytotoxic activity of supercritical CO2 extract of old man's beard in L929 fibrosarcoma cell line
VL  - 39
SP  - 30
EP  - 34
DO  - 10.5937/leksir1939030Z
ER  - 

@article{
author = "Žugić, Ana and Isaković, Aleksandra and Jeremić, Ivica and Savić, Snežana and Tadić, Vanja",
year = "2019",
abstract = "Old man’s beard (Usnea barbata) is one of the most investigated lichens which biological properties
(such as antimicrobial, anti-inflammatory and cytotoxic activities) have been proven in several scien-
tific studies. Stated properties of old man’s beard have been attributed mainly to its major secondary
metabolite - a dibenzofuran derivative, usnic acid. The aim of the current study was to examine cyto-
toxic activity of the quantified supercritical CO2 extract and pure usnic acid in parallel against L929
fibrosarcoma cells. Our results indicated the tested extract to be more toxic towards the investigated
cell line compared to usnic acid per se, despite the high amount of this compound (81.41% (w/w))
revealed in the extract using HPLC. Such result may be connected to the unidentified compounds
present in the supercritical CO2 extract that could be responsible for apoptosis and oxidative stress in
the exerted cytotoxicity of the investigated extract, but not usnic acid. Our study supports further in-
vestigations of supercritical CO2 extract of U. barbata as a prospective therapeutic agent with potential
relevance in the treatment of sarcoma.",
publisher = "Institut za proučavanje lekovitog bilja "Dr Josif Pančić", Beograd",
journal = "Lekovite sirovine",
title = "Cytotoxic activity of supercritical CO2 extract of old man's beard in L929 fibrosarcoma cell line",
volume = "39",
pages = "30-34",
doi = "10.5937/leksir1939030Z"
}

Žugić, A., Isaković, A., Jeremić, I., Savić, S.,& Tadić, V.. (2019). Cytotoxic activity of supercritical CO2 extract of old man's beard in L929 fibrosarcoma cell line. in Lekovite sirovine
Institut za proučavanje lekovitog bilja "Dr Josif Pančić", Beograd., 39, 30-34.
https://doi.org/10.5937/leksir1939030Z

Žugić A, Isaković A, Jeremić I, Savić S, Tadić V. Cytotoxic activity of supercritical CO2 extract of old man's beard in L929 fibrosarcoma cell line. in Lekovite sirovine. 2019;39:30-34.
doi:10.5937/leksir1939030Z .

Žugić, Ana, Isaković, Aleksandra, Jeremić, Ivica, Savić, Snežana, Tadić, Vanja, "Cytotoxic activity of supercritical CO2 extract of old man's beard in L929 fibrosarcoma cell line" in Lekovite sirovine, 39 (2019):30-34,
https://doi.org/10.5937/leksir1939030Z . .