TY  - JOUR
AU  - Husejnović, Maida Sljivic
AU  - Bergant, Martina
AU  - Janković, Saša
AU  - Zizek, Suzana
AU  - Smajlović, Aida
AU  - Softić, Adaleta
AU  - Musić, Omer
AU  - Antonijević, Biljana
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3213
AB  - Soil contamination by heavy metals is a serious global environmental problem, especially for developing countries. A large number of industrial plants, which continually pollute the environment, characterize Tuzla Canton, Bosnia and Herzegovina. The aim of this study was to assess the level of soil pollution by heavy metals and to estimate cytotoxicity and genotoxicity of soil leachates from this area. Lead (Pb), cadmium (Cd) and mercury (Hg) were analyzed by ICP-AES and AAS. Soil contamination was assessed using contamination factor, degree of contamination, geoaccumulation index and pollution load index. To determine the connection of variables and understanding their origin in soils, principal component analysis (PCA) and cluster analysis (CA) were used. The results indicate that Cd and Hg originated from natural and anthropogenic activities, while Pb is of anthropogenic origin. For toxicity evaluation, CaCo-2 and HaCaT cells were used. PrestoBlue assay was used for cytotoxicity testing, and cH2A. X for genotoxicity evaluation. Concerning cytotoxicity, Cd and Hg had a positive correlation with cytotoxicity in HaCaT cells, but only Hg induced cytotoxicity in CaCo-2 cells. We also demonstrate that soil leachates contaminated by heavy metals can induce genotoxicity in both used cell lines. According to these results, combining bioassays with standard physicochemical analysis can be useful for evaluating environmental and health risks more accurately. These results are important for developing proper management strategies to decrease pollution. This is one of the first studies from this area and an important indication of soil quality in Southeast Europe.
PB  - Springer, Dordrecht
T2  - Environmental Geochemistry and Health
T1  - Assessment of Pb, Cd and Hg soil contamination and its potential to cause cytotoxic and genotoxic effects in human cell lines (CaCo-2 and HaCaT)
VL  - 40
IS  - 4
SP  - 1557
EP  - 1572
DO  - 10.1007/s10653-018-0071-6
ER  - 

@article{
author = "Husejnović, Maida Sljivic and Bergant, Martina and Janković, Saša and Zizek, Suzana and Smajlović, Aida and Softić, Adaleta and Musić, Omer and Antonijević, Biljana",
year = "2018",
abstract = "Soil contamination by heavy metals is a serious global environmental problem, especially for developing countries. A large number of industrial plants, which continually pollute the environment, characterize Tuzla Canton, Bosnia and Herzegovina. The aim of this study was to assess the level of soil pollution by heavy metals and to estimate cytotoxicity and genotoxicity of soil leachates from this area. Lead (Pb), cadmium (Cd) and mercury (Hg) were analyzed by ICP-AES and AAS. Soil contamination was assessed using contamination factor, degree of contamination, geoaccumulation index and pollution load index. To determine the connection of variables and understanding their origin in soils, principal component analysis (PCA) and cluster analysis (CA) were used. The results indicate that Cd and Hg originated from natural and anthropogenic activities, while Pb is of anthropogenic origin. For toxicity evaluation, CaCo-2 and HaCaT cells were used. PrestoBlue assay was used for cytotoxicity testing, and cH2A. X for genotoxicity evaluation. Concerning cytotoxicity, Cd and Hg had a positive correlation with cytotoxicity in HaCaT cells, but only Hg induced cytotoxicity in CaCo-2 cells. We also demonstrate that soil leachates contaminated by heavy metals can induce genotoxicity in both used cell lines. According to these results, combining bioassays with standard physicochemical analysis can be useful for evaluating environmental and health risks more accurately. These results are important for developing proper management strategies to decrease pollution. This is one of the first studies from this area and an important indication of soil quality in Southeast Europe.",
publisher = "Springer, Dordrecht",
journal = "Environmental Geochemistry and Health",
title = "Assessment of Pb, Cd and Hg soil contamination and its potential to cause cytotoxic and genotoxic effects in human cell lines (CaCo-2 and HaCaT)",
volume = "40",
number = "4",
pages = "1557-1572",
doi = "10.1007/s10653-018-0071-6"
}

Husejnović, M. S., Bergant, M., Janković, S., Zizek, S., Smajlović, A., Softić, A., Musić, O.,& Antonijević, B.. (2018). Assessment of Pb, Cd and Hg soil contamination and its potential to cause cytotoxic and genotoxic effects in human cell lines (CaCo-2 and HaCaT). in Environmental Geochemistry and Health
Springer, Dordrecht., 40(4), 1557-1572.
https://doi.org/10.1007/s10653-018-0071-6

Husejnović MS, Bergant M, Janković S, Zizek S, Smajlović A, Softić A, Musić O, Antonijević B. Assessment of Pb, Cd and Hg soil contamination and its potential to cause cytotoxic and genotoxic effects in human cell lines (CaCo-2 and HaCaT). in Environmental Geochemistry and Health. 2018;40(4):1557-1572.
doi:10.1007/s10653-018-0071-6 .

Husejnović, Maida Sljivic, Bergant, Martina, Janković, Saša, Zizek, Suzana, Smajlović, Aida, Softić, Adaleta, Musić, Omer, Antonijević, Biljana, "Assessment of Pb, Cd and Hg soil contamination and its potential to cause cytotoxic and genotoxic effects in human cell lines (CaCo-2 and HaCaT)" in Environmental Geochemistry and Health, 40, no. 4 (2018):1557-1572,
https://doi.org/10.1007/s10653-018-0071-6 . .