TY  - JOUR
AU  - Krombholz, Richard
AU  - Fressle, Stefanie
AU  - Nikolić, Ines
AU  - Pantelić, Ivana
AU  - Savić, Snežana
AU  - Crevar-Sakač, Milkica
AU  - Lunter, Dominique
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4333
AB  - When it comes to skin penetration analysis of a topically applied formulation, the number of suitable methods is limited, and they often lack in spatial resolution. In vivo studies are pivotal, especially in the approval of a new product, but high costs and ethical difficulties are limiting factors. For that reason, good ex vivo models for testing skin penetration are crucial. In this study, caffeine was used as a hydrophilic model drug, applied as a 2% (w/w) hydrogel, to compare different techniques for skin penetration analysis. Confocal Raman microspectroscopy (CRM) and tape stripping with subsequent HPLC analysis were used to quantify caffeine. Experiments were performed ex vivo and in vivo. Furthermore, the effect of 5% (w/w) 1,2-pentanediol on caffeine skin penetration was tested, to compare those methods regarding their effectiveness in detecting differences between both formulations.
PB  - John Wiley and Sons Inc
T2  - Experimental Dermatology
T1  - ex vivo–in vivo comparison of drug penetration analysis by confocal Raman microspectroscopy and tape stripping
VL  - 31
IS  - 12
DO  - 10.1111/exd.14672
ER  - 

@article{
author = "Krombholz, Richard and Fressle, Stefanie and Nikolić, Ines and Pantelić, Ivana and Savić, Snežana and Crevar-Sakač, Milkica and Lunter, Dominique",
year = "2022",
abstract = "When it comes to skin penetration analysis of a topically applied formulation, the number of suitable methods is limited, and they often lack in spatial resolution. In vivo studies are pivotal, especially in the approval of a new product, but high costs and ethical difficulties are limiting factors. For that reason, good ex vivo models for testing skin penetration are crucial. In this study, caffeine was used as a hydrophilic model drug, applied as a 2% (w/w) hydrogel, to compare different techniques for skin penetration analysis. Confocal Raman microspectroscopy (CRM) and tape stripping with subsequent HPLC analysis were used to quantify caffeine. Experiments were performed ex vivo and in vivo. Furthermore, the effect of 5% (w/w) 1,2-pentanediol on caffeine skin penetration was tested, to compare those methods regarding their effectiveness in detecting differences between both formulations.",
publisher = "John Wiley and Sons Inc",
journal = "Experimental Dermatology",
title = "ex vivo–in vivo comparison of drug penetration analysis by confocal Raman microspectroscopy and tape stripping",
volume = "31",
number = "12",
doi = "10.1111/exd.14672"
}

Krombholz, R., Fressle, S., Nikolić, I., Pantelić, I., Savić, S., Crevar-Sakač, M.,& Lunter, D.. (2022). ex vivo–in vivo comparison of drug penetration analysis by confocal Raman microspectroscopy and tape stripping. in Experimental Dermatology
John Wiley and Sons Inc., 31(12).
https://doi.org/10.1111/exd.14672

Krombholz R, Fressle S, Nikolić I, Pantelić I, Savić S, Crevar-Sakač M, Lunter D. ex vivo–in vivo comparison of drug penetration analysis by confocal Raman microspectroscopy and tape stripping. in Experimental Dermatology. 2022;31(12).
doi:10.1111/exd.14672 .

Krombholz, Richard, Fressle, Stefanie, Nikolić, Ines, Pantelić, Ivana, Savić, Snežana, Crevar-Sakač, Milkica, Lunter, Dominique, "ex vivo–in vivo comparison of drug penetration analysis by confocal Raman microspectroscopy and tape stripping" in Experimental Dermatology, 31, no. 12 (2022),
https://doi.org/10.1111/exd.14672 . .