TY  - JOUR
AU  - Stevanović, Milena
AU  - Đošić, Marija
AU  - Janković, Ana
AU  - Kojić, Vesna
AU  - Vukašinović-Sekulić, Maja
AU  - Stojanović, Jovica
AU  - Odović, Jadranka
AU  - Crevar-Sakač, Milkica
AU  - Kyong Yop, Rhee
AU  - Mišković-Stanković, Vesna
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3657
AB  - Electrophoretic deposition process (EPD) was successfully used for obtaining graphene (Gr)-reinforced composite coating based on hydroxyapatite (HAP), chitosan (CS), and antibiotic gentamicin (Gent), from aqueous suspension. The deposition process was performed as a single step process at a constant voltage (5 V, deposition time 12 min) on pure titanium foils. The influence of graphene was examined through detailed physicochemical and biological characterization. Fourier transform infrared spectroscopy, field emission scanning electron microscopy, thermogravimetric analysis, X-ray diffraction, Raman, and X-ray photoelectron analyses confirmed the formation of composite HAP/CS/Gr and HAP/CS/Gr/Gent coatings on Ti. Obtained coatings had porous, uniform, fracture-free surfaces, suggesting strong interfacial interaction between HAP, CS, and Gr. Large specific area of graphene enabled strong bonding with chitosan, acting as nanofiller throughout the polymer matrix. Gentamicin addition strongly improved the antibacterial activity of HAP/CS/Gr/Gent coating that was confirmed by antibacterial activity kinetics in suspension and agar diffusion testing, while results indicated more pronounced antibacterial effect against Staphylococcus aureus (bactericidal, viable cells number reduction >3 logarithmic units) compared to Escherichia coli (bacteriostatic, <3 logarithmic units). MTT assay indicated low cytotoxicity (75% cell viability) against MRC-5 and L929 (70% cell viability) tested cell lines, indicating good biocompatibility of HAP/CS/Gr/Gent coating. Therefore, electrodeposited HAP/CS/Gr/Gent coating on Ti can be considered as a prospective material for bone tissue engineering as a hard tissue implant.
PB  - Wiley Periodicals, LLC.
T2  - Journal of Biomedical Materials Research - Part A
T1  - Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering
VL  - 108
IS  - 11
SP  - 2175
EP  - 2189
DO  - 10.1002/jbm.a.36974
ER  - 

@article{
author = "Stevanović, Milena and Đošić, Marija and Janković, Ana and Kojić, Vesna and Vukašinović-Sekulić, Maja and Stojanović, Jovica and Odović, Jadranka and Crevar-Sakač, Milkica and Kyong Yop, Rhee and Mišković-Stanković, Vesna",
year = "2020",
abstract = "Electrophoretic deposition process (EPD) was successfully used for obtaining graphene (Gr)-reinforced composite coating based on hydroxyapatite (HAP), chitosan (CS), and antibiotic gentamicin (Gent), from aqueous suspension. The deposition process was performed as a single step process at a constant voltage (5 V, deposition time 12 min) on pure titanium foils. The influence of graphene was examined through detailed physicochemical and biological characterization. Fourier transform infrared spectroscopy, field emission scanning electron microscopy, thermogravimetric analysis, X-ray diffraction, Raman, and X-ray photoelectron analyses confirmed the formation of composite HAP/CS/Gr and HAP/CS/Gr/Gent coatings on Ti. Obtained coatings had porous, uniform, fracture-free surfaces, suggesting strong interfacial interaction between HAP, CS, and Gr. Large specific area of graphene enabled strong bonding with chitosan, acting as nanofiller throughout the polymer matrix. Gentamicin addition strongly improved the antibacterial activity of HAP/CS/Gr/Gent coating that was confirmed by antibacterial activity kinetics in suspension and agar diffusion testing, while results indicated more pronounced antibacterial effect against Staphylococcus aureus (bactericidal, viable cells number reduction >3 logarithmic units) compared to Escherichia coli (bacteriostatic, <3 logarithmic units). MTT assay indicated low cytotoxicity (75% cell viability) against MRC-5 and L929 (70% cell viability) tested cell lines, indicating good biocompatibility of HAP/CS/Gr/Gent coating. Therefore, electrodeposited HAP/CS/Gr/Gent coating on Ti can be considered as a prospective material for bone tissue engineering as a hard tissue implant.",
publisher = "Wiley Periodicals, LLC.",
journal = "Journal of Biomedical Materials Research - Part A",
title = "Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering",
volume = "108",
number = "11",
pages = "2175-2189",
doi = "10.1002/jbm.a.36974"
}

Stevanović, M., Đošić, M., Janković, A., Kojić, V., Vukašinović-Sekulić, M., Stojanović, J., Odović, J., Crevar-Sakač, M., Kyong Yop, R.,& Mišković-Stanković, V.. (2020). Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering. in Journal of Biomedical Materials Research - Part A
Wiley Periodicals, LLC.., 108(11), 2175-2189.
https://doi.org/10.1002/jbm.a.36974

Stevanović M, Đošić M, Janković A, Kojić V, Vukašinović-Sekulić M, Stojanović J, Odović J, Crevar-Sakač M, Kyong Yop R, Mišković-Stanković V. Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering. in Journal of Biomedical Materials Research - Part A. 2020;108(11):2175-2189.
doi:10.1002/jbm.a.36974 .

Stevanović, Milena, Đošić, Marija, Janković, Ana, Kojić, Vesna, Vukašinović-Sekulić, Maja, Stojanović, Jovica, Odović, Jadranka, Crevar-Sakač, Milkica, Kyong Yop, Rhee, Mišković-Stanković, Vesna, "Antibacterial graphene-based hydroxyapatite/chitosan coating with gentamicin for potential applications in bone tissue engineering" in Journal of Biomedical Materials Research - Part A, 108, no. 11 (2020):2175-2189,
https://doi.org/10.1002/jbm.a.36974 . .

TY  - JOUR
AU  - Osmokrović, Andrea
AU  - Jančić, Ivan
AU  - Janković-Castvan, Ivona
AU  - Petrović, Predrag
AU  - Milenković, Marina
AU  - Obradović, Bojana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3308
AB  - Composites based on Zn-alginate hydrogels in the form of beads were produced by extrusion of a suspension containing 0.5 % w/w of alginate and 20 % w/w of activated charcoal (AC) with the intent to simultaneously release two active agents, Zn2+ and AC particles, in a physiological-like environment. The obtained composite beads were analyzed by FE-SEM and characterized regarding textural parameters, as well as Zn2+ and AC release kinetics in the physiological saline solution. Zn(2+)ions were quickly released reaching the equilibrium concentration within the first hour in the contrary to the release of AC particles, which was described by internal diffusion with the apparent diffusion coefficient of approximately 10(-13) m(2) s(-1). Potential functionality of the obtained beads was evaluated regarding antibacterial activity in suspensions of the standard bacterial strain Escherichia coli 25922. The observed strong bactericidal effects were related to the quick release of Zn2+ that was not affected by AC. Thus, taking into account results of this study, as well as high sorption capacity of alginate hydrogel, efficiency of AC to adsorb malodor and tissue degradation products and positive effects of Zn2+ on wound healing, the obtained composites have shown promising potentials for applications as multifunctional wound dressings.
PB  - Savez hemijskih inženjera, Beograd
T2  - Hemijska industrija
T1  - Novel composite zinc-alginate hydrogels with activated charcoal aimed for potential applications in multifunctional primary wound dressings
VL  - 73
IS  - 1
SP  - 37
EP  - 46
DO  - 10.2298/HEMIND180629003O
ER  - 

@article{
author = "Osmokrović, Andrea and Jančić, Ivan and Janković-Castvan, Ivona and Petrović, Predrag and Milenković, Marina and Obradović, Bojana",
year = "2019",
abstract = "Composites based on Zn-alginate hydrogels in the form of beads were produced by extrusion of a suspension containing 0.5 % w/w of alginate and 20 % w/w of activated charcoal (AC) with the intent to simultaneously release two active agents, Zn2+ and AC particles, in a physiological-like environment. The obtained composite beads were analyzed by FE-SEM and characterized regarding textural parameters, as well as Zn2+ and AC release kinetics in the physiological saline solution. Zn(2+)ions were quickly released reaching the equilibrium concentration within the first hour in the contrary to the release of AC particles, which was described by internal diffusion with the apparent diffusion coefficient of approximately 10(-13) m(2) s(-1). Potential functionality of the obtained beads was evaluated regarding antibacterial activity in suspensions of the standard bacterial strain Escherichia coli 25922. The observed strong bactericidal effects were related to the quick release of Zn2+ that was not affected by AC. Thus, taking into account results of this study, as well as high sorption capacity of alginate hydrogel, efficiency of AC to adsorb malodor and tissue degradation products and positive effects of Zn2+ on wound healing, the obtained composites have shown promising potentials for applications as multifunctional wound dressings.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Hemijska industrija",
title = "Novel composite zinc-alginate hydrogels with activated charcoal aimed for potential applications in multifunctional primary wound dressings",
volume = "73",
number = "1",
pages = "37-46",
doi = "10.2298/HEMIND180629003O"
}

Osmokrović, A., Jančić, I., Janković-Castvan, I., Petrović, P., Milenković, M.,& Obradović, B.. (2019). Novel composite zinc-alginate hydrogels with activated charcoal aimed for potential applications in multifunctional primary wound dressings. in Hemijska industrija
Savez hemijskih inženjera, Beograd., 73(1), 37-46.
https://doi.org/10.2298/HEMIND180629003O

Osmokrović A, Jančić I, Janković-Castvan I, Petrović P, Milenković M, Obradović B. Novel composite zinc-alginate hydrogels with activated charcoal aimed for potential applications in multifunctional primary wound dressings. in Hemijska industrija. 2019;73(1):37-46.
doi:10.2298/HEMIND180629003O .

Osmokrović, Andrea, Jančić, Ivan, Janković-Castvan, Ivona, Petrović, Predrag, Milenković, Marina, Obradović, Bojana, "Novel composite zinc-alginate hydrogels with activated charcoal aimed for potential applications in multifunctional primary wound dressings" in Hemijska industrija, 73, no. 1 (2019):37-46,
https://doi.org/10.2298/HEMIND180629003O . .

TY  - JOUR
AU  - Osmokrović, Andrea
AU  - Jančić, Ivan
AU  - Vunduk, Jovana
AU  - Petrović, Predrag
AU  - Milenković, Marina
AU  - Obradović, Bojana
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3240
AB  - New composites based on Ca-alginate hydrogels were produced that release activated charcoal (AC) particles with adsorbed povidone iodine (PVP-I) as a model antimicrobial substance in a physiological-like environment. Composite beads with different alginate (0.5-1.5% w/w) and AC (1-20% w/w) concentrations were analyzed by FE-SEM and characterized regarding textural parameters, swelling, and AC release kinetics. PVP-I was easily adsorbed onto AC particles within the optimized beads (0.5% w/w alginate, 20% w/w AC) as indicated by UV-vis spectroscopy, EDX and FT-IR analyses. The obtained beads have shown strong bactericidal effects against two standard bacterial strains (Staphylococcus aureus and Pseudomonas aeruginosa) and clinical multi-resistant wound isolates (MRSA, Escherichia coli, Pseudomonas aeruginosa, Enterococcus faecalis and Proteus mirabilis) and, at the same time, exhibited negligible PVP-I desorption in physiological saline solution. Thus, the obtained composites could provide utilization of potent antiseptics such as iodine, in wound dressings, without the concern of systemic absorption.
PB  - Elsevier Sci Ltd, Oxford
T2  - Carbohydrate Polymers
T1  - Achieving high antimicrobial activity: Composite alginate hydrogel beads releasing activated charcoal with an immobilized active agent
VL  - 196
SP  - 279
EP  - 288
DO  - 10.1016/j.carbpol.2018.05.045
ER  - 

@article{
author = "Osmokrović, Andrea and Jančić, Ivan and Vunduk, Jovana and Petrović, Predrag and Milenković, Marina and Obradović, Bojana",
year = "2018",
abstract = "New composites based on Ca-alginate hydrogels were produced that release activated charcoal (AC) particles with adsorbed povidone iodine (PVP-I) as a model antimicrobial substance in a physiological-like environment. Composite beads with different alginate (0.5-1.5% w/w) and AC (1-20% w/w) concentrations were analyzed by FE-SEM and characterized regarding textural parameters, swelling, and AC release kinetics. PVP-I was easily adsorbed onto AC particles within the optimized beads (0.5% w/w alginate, 20% w/w AC) as indicated by UV-vis spectroscopy, EDX and FT-IR analyses. The obtained beads have shown strong bactericidal effects against two standard bacterial strains (Staphylococcus aureus and Pseudomonas aeruginosa) and clinical multi-resistant wound isolates (MRSA, Escherichia coli, Pseudomonas aeruginosa, Enterococcus faecalis and Proteus mirabilis) and, at the same time, exhibited negligible PVP-I desorption in physiological saline solution. Thus, the obtained composites could provide utilization of potent antiseptics such as iodine, in wound dressings, without the concern of systemic absorption.",
publisher = "Elsevier Sci Ltd, Oxford",
journal = "Carbohydrate Polymers",
title = "Achieving high antimicrobial activity: Composite alginate hydrogel beads releasing activated charcoal with an immobilized active agent",
volume = "196",
pages = "279-288",
doi = "10.1016/j.carbpol.2018.05.045"
}

Osmokrović, A., Jančić, I., Vunduk, J., Petrović, P., Milenković, M.,& Obradović, B.. (2018). Achieving high antimicrobial activity: Composite alginate hydrogel beads releasing activated charcoal with an immobilized active agent. in Carbohydrate Polymers
Elsevier Sci Ltd, Oxford., 196, 279-288.
https://doi.org/10.1016/j.carbpol.2018.05.045

Osmokrović A, Jančić I, Vunduk J, Petrović P, Milenković M, Obradović B. Achieving high antimicrobial activity: Composite alginate hydrogel beads releasing activated charcoal with an immobilized active agent. in Carbohydrate Polymers. 2018;196:279-288.
doi:10.1016/j.carbpol.2018.05.045 .

Osmokrović, Andrea, Jančić, Ivan, Vunduk, Jovana, Petrović, Predrag, Milenković, Marina, Obradović, Bojana, "Achieving high antimicrobial activity: Composite alginate hydrogel beads releasing activated charcoal with an immobilized active agent" in Carbohydrate Polymers, 196 (2018):279-288,
https://doi.org/10.1016/j.carbpol.2018.05.045 . .

TY  - JOUR
AU  - Stojkovska, Jasmina
AU  - Đurđević, Željka
AU  - Jančić, Ivan
AU  - Bufan, Biljana
AU  - Milenković, Marina
AU  - Janković, Radmila
AU  - Mišković-Stanković, Vesna
AU  - Obradović, Bojana
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3169
AB  - In the present study, possibilities for using novel nanocomposites based on alginate and silver nanoparticles for wound treatment were investigated in a second-degree thermal burn model in Wistar rats. Silver nanoparticles (AgNPs) were electrochemically synthesized in alginate solutions that were further utilized to obtain the Ag/alginate solution and microfibers for subsequent in vivo studies. Daily applications of the Ag/alginate colloid solution, containing AgNPs, alginate and ascorbic acid (G3), wet Ag/alginate microfibers containing AgNPs (G5) and dry Ag/alginate microfibers containing AgNPs (G6) were compared to treatments with a commercial cream containing silver sulfadiazine (G2) and a commercial Ca-alginate wound dressing containing silver ions (G4), as well as to the untreated controls (G1). Results of the in vivo study have shown faster healing in treated wounds, which completely healed on day 19 (G4, G5 and G6) and 21 (G2 and G3) after the thermal injury, while the period for complete reepitelization of untreated wounds (G1) was 25 days. The macroscopic analysis has shown that scabs fell off between day 10 and 12 after the thermal injury induction in treated groups, whereas between day 15 and 16 in the control group. These macroscopic findings were supported by the results of histopathological analyses, which have shown enhanced granulation and reepithelization, reduced inflammation and improved organization of the extracellular matrix in treated groups without adverse effects. Among the treated groups, dressings based on Ca-alginate (G4-G6) induced enhanced healing as compared to the other two groups (G2, G3), which could be attributed to additional stimuli of released Ca2+. The obtained results indicated potentials of novel nanocomposites based on alginate and AgNPs for therapeutic applications in wound treatments.
PB  - Sage Publications Ltd, London
T2  - Journal of Biomaterials Applications
T1  - Comparative in vivo evaluation of novel formulations based on alginate and silver nanoparticles for wound treatments
VL  - 32
IS  - 9
SP  - 1197
EP  - 1211
DO  - 10.1177/0885328218759564
ER  - 

@article{
author = "Stojkovska, Jasmina and Đurđević, Željka and Jančić, Ivan and Bufan, Biljana and Milenković, Marina and Janković, Radmila and Mišković-Stanković, Vesna and Obradović, Bojana",
year = "2018",
abstract = "In the present study, possibilities for using novel nanocomposites based on alginate and silver nanoparticles for wound treatment were investigated in a second-degree thermal burn model in Wistar rats. Silver nanoparticles (AgNPs) were electrochemically synthesized in alginate solutions that were further utilized to obtain the Ag/alginate solution and microfibers for subsequent in vivo studies. Daily applications of the Ag/alginate colloid solution, containing AgNPs, alginate and ascorbic acid (G3), wet Ag/alginate microfibers containing AgNPs (G5) and dry Ag/alginate microfibers containing AgNPs (G6) were compared to treatments with a commercial cream containing silver sulfadiazine (G2) and a commercial Ca-alginate wound dressing containing silver ions (G4), as well as to the untreated controls (G1). Results of the in vivo study have shown faster healing in treated wounds, which completely healed on day 19 (G4, G5 and G6) and 21 (G2 and G3) after the thermal injury, while the period for complete reepitelization of untreated wounds (G1) was 25 days. The macroscopic analysis has shown that scabs fell off between day 10 and 12 after the thermal injury induction in treated groups, whereas between day 15 and 16 in the control group. These macroscopic findings were supported by the results of histopathological analyses, which have shown enhanced granulation and reepithelization, reduced inflammation and improved organization of the extracellular matrix in treated groups without adverse effects. Among the treated groups, dressings based on Ca-alginate (G4-G6) induced enhanced healing as compared to the other two groups (G2, G3), which could be attributed to additional stimuli of released Ca2+. The obtained results indicated potentials of novel nanocomposites based on alginate and AgNPs for therapeutic applications in wound treatments.",
publisher = "Sage Publications Ltd, London",
journal = "Journal of Biomaterials Applications",
title = "Comparative in vivo evaluation of novel formulations based on alginate and silver nanoparticles for wound treatments",
volume = "32",
number = "9",
pages = "1197-1211",
doi = "10.1177/0885328218759564"
}

Stojkovska, J., Đurđević, Ž., Jančić, I., Bufan, B., Milenković, M., Janković, R., Mišković-Stanković, V.,& Obradović, B.. (2018). Comparative in vivo evaluation of novel formulations based on alginate and silver nanoparticles for wound treatments. in Journal of Biomaterials Applications
Sage Publications Ltd, London., 32(9), 1197-1211.
https://doi.org/10.1177/0885328218759564

Stojkovska J, Đurđević Ž, Jančić I, Bufan B, Milenković M, Janković R, Mišković-Stanković V, Obradović B. Comparative in vivo evaluation of novel formulations based on alginate and silver nanoparticles for wound treatments. in Journal of Biomaterials Applications. 2018;32(9):1197-1211.
doi:10.1177/0885328218759564 .

Stojkovska, Jasmina, Đurđević, Željka, Jančić, Ivan, Bufan, Biljana, Milenković, Marina, Janković, Radmila, Mišković-Stanković, Vesna, Obradović, Bojana, "Comparative in vivo evaluation of novel formulations based on alginate and silver nanoparticles for wound treatments" in Journal of Biomaterials Applications, 32, no. 9 (2018):1197-1211,
https://doi.org/10.1177/0885328218759564 . .

TY  - CONF
AU  - Zvicer, Jovana S
AU  - Girandon, L.
AU  - Potocar, U.
AU  - Froehlich, M.
AU  - Jančić, Ivan
AU  - Bufan, Biljana
AU  - Milenković, Marina
AU  - Stojkovska, J.
AU  - Mišković-Stanković, Vesna
AU  - Obradović, Bojana
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2220
PB  - Wiley-Blackwell, Hoboken
C3  - Journal of Tissue Engineering and Regenerative Medicine
T1  - Cytotoxicity studies of novel Ag/alginate nanocomposites aimed for wound treatment
VL  - 8
IS  - Supplement 1
SP  - 345
EP  - 345
DO  - 10.1002/term.1932
ER  - 

@conference{
author = "Zvicer, Jovana S and Girandon, L. and Potocar, U. and Froehlich, M. and Jančić, Ivan and Bufan, Biljana and Milenković, Marina and Stojkovska, J. and Mišković-Stanković, Vesna and Obradović, Bojana",
year = "2014",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Journal of Tissue Engineering and Regenerative Medicine",
title = "Cytotoxicity studies of novel Ag/alginate nanocomposites aimed for wound treatment",
volume = "8",
number = "Supplement 1",
pages = "345-345",
doi = "10.1002/term.1932"
}

Zvicer, J. S., Girandon, L., Potocar, U., Froehlich, M., Jančić, I., Bufan, B., Milenković, M., Stojkovska, J., Mišković-Stanković, V.,& Obradović, B.. (2014). Cytotoxicity studies of novel Ag/alginate nanocomposites aimed for wound treatment. in Journal of Tissue Engineering and Regenerative Medicine
Wiley-Blackwell, Hoboken., 8(Supplement 1), 345-345.
https://doi.org/10.1002/term.1932

Zvicer JS, Girandon L, Potocar U, Froehlich M, Jančić I, Bufan B, Milenković M, Stojkovska J, Mišković-Stanković V, Obradović B. Cytotoxicity studies of novel Ag/alginate nanocomposites aimed for wound treatment. in Journal of Tissue Engineering and Regenerative Medicine. 2014;8(Supplement 1):345-345.
doi:10.1002/term.1932 .

Zvicer, Jovana S, Girandon, L., Potocar, U., Froehlich, M., Jančić, Ivan, Bufan, Biljana, Milenković, Marina, Stojkovska, J., Mišković-Stanković, Vesna, Obradović, Bojana, "Cytotoxicity studies of novel Ag/alginate nanocomposites aimed for wound treatment" in Journal of Tissue Engineering and Regenerative Medicine, 8, no. Supplement 1 (2014):345-345,
https://doi.org/10.1002/term.1932 . .