TY  - JOUR
AU  - Madžgalj, Valerija
AU  - Petrović, Aleksandar
AU  - Čakar, Uroš
AU  - Maraš, Vesna
AU  - Sofrenić, Ivana
AU  - Tešević, Vele
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4428
AB  - This study aimed to show aromatic profile of wines produced from two autochthonous grape cultivars Krstač (K) and Žižak (Z). During the wine production two enzymatic preparations (EP) Lallzyme cuvee blanc (CB) and Lallzyme enzymatic preparation β (EB) and different time of skin contact (4 and 8 h) were applied. Aromatic compounds were detected by GC/FID–MS analysis. Significantly higher content of total detected aromatic compounds compared to appropriate controls (168.54 and 161.72 mg L-1 ) was observed for K EB4h (176.33 mg L -1 ) and Z CB4h (177.29 mg L -1 ) wines. Skin contact and usage of EP mostly increased content of 2-phenylethyl and isoamyl alcohols. Wines from both varieties showed higher content of hexanoic and octanoic acids compared to the control. It is interesting to emphasize that content of esters that are responsible for fruity aroma of wine which is important for plea- sant taste (isoamyl acetate – banana, ethyl hexanoate – ripe banana, 2-phenyl- ethyl acetate – powerful fruity rose like) were increased in all samples com- pared to the controls. The highest grades, after sensory evaluation, were obtained for K EB 8h (18.0 out of 20.0) and Z CB 8h (18.2 out of 20.0).
AB  - Ова студија је имала за циљ да прикаже профиле ароматичних једињења вина про- изведених од аутохтоних сорти грожђа Крстач (K) и Жижак (Z). Током производње вина од обе сорте коришћени су ензимски препарати (EP): Lallzyme cuvee blanc (CB), Lall- zyme enzymatic preparation β (EB) и различито време контакта покожице (4 и 8 h). Аро- матична једињења су анализирана GC/FID–MS техником. За вина K ЕВ4h (176,33 mg L -1 ) и Z CB4h (177,29 mg L -1 ) уочава се значајно већи садржај укупних ароматичних једињења у поређењу са одговарајућим контролним винима (168,54 and 161,72 mg L -1 ). Про- дужење времена контакта покожице и употреба ЕP углавном повећава садржај 2-фенил- етил- и изоамил-алкохола. Вина обе сорте су показала већи садржај хексанске и октан- ске киселине у односу на контролна вина. Занимљиво је поменути да је у свим узорцима повећан садржај естара који су одговорни за воћну арому вина, која је заслужна за при- јатан укус (изоамил-ацетат – банана, етил-хексаноат – зрела банана, 2-фенилетил-аце- тат – јак воћни мирис руже), у поређењу са контролним винима. Највише оцене, након сензорног оцењивања, добијене су за K EB 8h (18,0 од максималних 20,0) и Z CB 8h (18,2 од максималних 20,0).
PB  - Serbian Chemical Society
T2  - Journal of the Serbian Chemical Society
T1  - The influence of different enzymatic preparations and skin contact time on aromatic profile of wines produced from autochthonous grape varieties Krstac and Zizak
T1  - Утицај различитих ензимских третмана и времена контакта покожице на ароматске профиле вина произведених од аутохтоних сорти грожђа Крстач и Жижак
VL  - 88
IS  - 1
SP  - 11
EP  - 23
DO  - 10.2298/JSC220311056M
ER  - 

@article{
author = "Madžgalj, Valerija and Petrović, Aleksandar and Čakar, Uroš and Maraš, Vesna and Sofrenić, Ivana and Tešević, Vele",
year = "2023",
abstract = "This study aimed to show aromatic profile of wines produced from two autochthonous grape cultivars Krstač (K) and Žižak (Z). During the wine production two enzymatic preparations (EP) Lallzyme cuvee blanc (CB) and Lallzyme enzymatic preparation β (EB) and different time of skin contact (4 and 8 h) were applied. Aromatic compounds were detected by GC/FID–MS analysis. Significantly higher content of total detected aromatic compounds compared to appropriate controls (168.54 and 161.72 mg L-1 ) was observed for K EB4h (176.33 mg L -1 ) and Z CB4h (177.29 mg L -1 ) wines. Skin contact and usage of EP mostly increased content of 2-phenylethyl and isoamyl alcohols. Wines from both varieties showed higher content of hexanoic and octanoic acids compared to the control. It is interesting to emphasize that content of esters that are responsible for fruity aroma of wine which is important for plea- sant taste (isoamyl acetate – banana, ethyl hexanoate – ripe banana, 2-phenyl- ethyl acetate – powerful fruity rose like) were increased in all samples com- pared to the controls. The highest grades, after sensory evaluation, were obtained for K EB 8h (18.0 out of 20.0) and Z CB 8h (18.2 out of 20.0)., Ова студија је имала за циљ да прикаже профиле ароматичних једињења вина про- изведених од аутохтоних сорти грожђа Крстач (K) и Жижак (Z). Током производње вина од обе сорте коришћени су ензимски препарати (EP): Lallzyme cuvee blanc (CB), Lall- zyme enzymatic preparation β (EB) и различито време контакта покожице (4 и 8 h). Аро- матична једињења су анализирана GC/FID–MS техником. За вина K ЕВ4h (176,33 mg L -1 ) и Z CB4h (177,29 mg L -1 ) уочава се значајно већи садржај укупних ароматичних једињења у поређењу са одговарајућим контролним винима (168,54 and 161,72 mg L -1 ). Про- дужење времена контакта покожице и употреба ЕP углавном повећава садржај 2-фенил- етил- и изоамил-алкохола. Вина обе сорте су показала већи садржај хексанске и октан- ске киселине у односу на контролна вина. Занимљиво је поменути да је у свим узорцима повећан садржај естара који су одговорни за воћну арому вина, која је заслужна за при- јатан укус (изоамил-ацетат – банана, етил-хексаноат – зрела банана, 2-фенилетил-аце- тат – јак воћни мирис руже), у поређењу са контролним винима. Највише оцене, након сензорног оцењивања, добијене су за K EB 8h (18,0 од максималних 20,0) и Z CB 8h (18,2 од максималних 20,0).",
publisher = "Serbian Chemical Society",
journal = "Journal of the Serbian Chemical Society",
title = "The influence of different enzymatic preparations and skin contact time on aromatic profile of wines produced from autochthonous grape varieties Krstac and Zizak, Утицај различитих ензимских третмана и времена контакта покожице на ароматске профиле вина произведених од аутохтоних сорти грожђа Крстач и Жижак",
volume = "88",
number = "1",
pages = "11-23",
doi = "10.2298/JSC220311056M"
}

Madžgalj, V., Petrović, A., Čakar, U., Maraš, V., Sofrenić, I.,& Tešević, V.. (2023). The influence of different enzymatic preparations and skin contact time on aromatic profile of wines produced from autochthonous grape varieties Krstac and Zizak. in Journal of the Serbian Chemical Society
Serbian Chemical Society., 88(1), 11-23.
https://doi.org/10.2298/JSC220311056M

Madžgalj V, Petrović A, Čakar U, Maraš V, Sofrenić I, Tešević V. The influence of different enzymatic preparations and skin contact time on aromatic profile of wines produced from autochthonous grape varieties Krstac and Zizak. in Journal of the Serbian Chemical Society. 2023;88(1):11-23.
doi:10.2298/JSC220311056M .

Madžgalj, Valerija, Petrović, Aleksandar, Čakar, Uroš, Maraš, Vesna, Sofrenić, Ivana, Tešević, Vele, "The influence of different enzymatic preparations and skin contact time on aromatic profile of wines produced from autochthonous grape varieties Krstac and Zizak" in Journal of the Serbian Chemical Society, 88, no. 1 (2023):11-23,
https://doi.org/10.2298/JSC220311056M . .

TY  - JOUR
AU  - Ivković, Branka
AU  - Opačić, Dragan
AU  - Džudović, Boris
AU  - Crevar, Milkica
AU  - Gojković-Bukarica, Ljiljana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4306
AB  - It is well known that the presence of different chemical groups in drug molecules influences their pharmacological properties. The aim of our study is to investigate whether newly synthesized derivatives of propafenone, with changes in benzyl moiety, have a different effect upon arrhythmia, compared to propafenone. 5OCl-PF and 5OF-PF are derivatives of propafenone with-Cl or –F substituent on the ortho position of the benzyl moiety. For verification of their antiarrhythmic effect, we used an in vivo rat model of aconitine-induced arrhythmia. 5OCl-PF speeded the appearance of supraventricular premature beats (SVPB) and death more than aconitine. All animals treated with 5OCl-PF developed ventricular premature beats in salvos (VPBS), bigeminies (VPBB) and paroxysmal ventricular tachycardia (PVT). 5OF-PF had a negative chronotropic effect and potentiated atrial excitability (more SVPB). It had a positive effect on the occurrence and onset time of supraventricular tachycardia, VPBS, and PVT. Based on the obtained results, it can be concluded that newly synthesized propafenone derivatives have no better antiarrhythmic effect than the parent compound. In the future, our research will be focused on the synthesis of different derivatives and examining their antiarrhythmic effects.
AB  - Dobro je poznato da prisustvo različitih hemijskih grupa u molekulima leka utiče na
njegova farmakološka svojstva. Cilj našeg istraživanja je ispitati da li novosintetisani derivati
propafenona, s promenama u benzilnoj grupi, imaju drugačiji efekat na aritmiju u odnosu na
propafenon. 5OCl-PF i 5OF-PF su derivati propafenona sa -Cl ili –F supstituentom na orto
položaju benzilnog dela. Za proveru njihovog antiaritmičnog efekta koristili smo in vivo model
na pacovima sa aritmijom izazvanom akonitinom. 5OCl-PF je ubrzao pojavu
supraventrikularnih prevremenih otkucaja (SVPB) i smrt više nego akonitin. Sve životinje
lečene sa 5OCl-PF razvile su ventrikularne prevremene otkucaje (VPBS i VPBB) i
paroksizmalnu ventrikularnu tahikardiju (PVT). 5OF-PF je imao negativan hronotropni efekat i
potencirao atrijalnu ekscitabilnost (više SVPB). Pozitivno je uticao na pojavu i vreme početka
supraventrikularne tahikardije, VPBS i PVT. Na osnovu dobijenih rezultata se može zaključiti
da novosintetisani derivati propafenona nemaju bolji antiaritmijski efekat od polaznog
jedinjenja. U budućnosti, istraživanje će biti usmereno ka sintezi hemijski drugačijih derivata i
ispitivanju njihovog antiaritmijskog efekta.
PB  - Pharmaceutical Association of Serbia
T2  - Arhiv za farmaciju
T1  - Antiarrhythmic effects of newly developed propafenone derivatives
T1  - Antiaritmički efekti novosintetisanih derivata
propafenona
VL  - 72
IS  - 4
SP  - 392
EP  - 412
DO  - 10.5937/arhfarm72-37114
ER  - 

@article{
author = "Ivković, Branka and Opačić, Dragan and Džudović, Boris and Crevar, Milkica and Gojković-Bukarica, Ljiljana",
year = "2022",
abstract = "It is well known that the presence of different chemical groups in drug molecules influences their pharmacological properties. The aim of our study is to investigate whether newly synthesized derivatives of propafenone, with changes in benzyl moiety, have a different effect upon arrhythmia, compared to propafenone. 5OCl-PF and 5OF-PF are derivatives of propafenone with-Cl or –F substituent on the ortho position of the benzyl moiety. For verification of their antiarrhythmic effect, we used an in vivo rat model of aconitine-induced arrhythmia. 5OCl-PF speeded the appearance of supraventricular premature beats (SVPB) and death more than aconitine. All animals treated with 5OCl-PF developed ventricular premature beats in salvos (VPBS), bigeminies (VPBB) and paroxysmal ventricular tachycardia (PVT). 5OF-PF had a negative chronotropic effect and potentiated atrial excitability (more SVPB). It had a positive effect on the occurrence and onset time of supraventricular tachycardia, VPBS, and PVT. Based on the obtained results, it can be concluded that newly synthesized propafenone derivatives have no better antiarrhythmic effect than the parent compound. In the future, our research will be focused on the synthesis of different derivatives and examining their antiarrhythmic effects., Dobro je poznato da prisustvo različitih hemijskih grupa u molekulima leka utiče na
njegova farmakološka svojstva. Cilj našeg istraživanja je ispitati da li novosintetisani derivati
propafenona, s promenama u benzilnoj grupi, imaju drugačiji efekat na aritmiju u odnosu na
propafenon. 5OCl-PF i 5OF-PF su derivati propafenona sa -Cl ili –F supstituentom na orto
položaju benzilnog dela. Za proveru njihovog antiaritmičnog efekta koristili smo in vivo model
na pacovima sa aritmijom izazvanom akonitinom. 5OCl-PF je ubrzao pojavu
supraventrikularnih prevremenih otkucaja (SVPB) i smrt više nego akonitin. Sve životinje
lečene sa 5OCl-PF razvile su ventrikularne prevremene otkucaje (VPBS i VPBB) i
paroksizmalnu ventrikularnu tahikardiju (PVT). 5OF-PF je imao negativan hronotropni efekat i
potencirao atrijalnu ekscitabilnost (više SVPB). Pozitivno je uticao na pojavu i vreme početka
supraventrikularne tahikardije, VPBS i PVT. Na osnovu dobijenih rezultata se može zaključiti
da novosintetisani derivati propafenona nemaju bolji antiaritmijski efekat od polaznog
jedinjenja. U budućnosti, istraživanje će biti usmereno ka sintezi hemijski drugačijih derivata i
ispitivanju njihovog antiaritmijskog efekta.",
publisher = "Pharmaceutical Association of Serbia",
journal = "Arhiv za farmaciju",
title = "Antiarrhythmic effects of newly developed propafenone derivatives, Antiaritmički efekti novosintetisanih derivata
propafenona",
volume = "72",
number = "4",
pages = "392-412",
doi = "10.5937/arhfarm72-37114"
}

Ivković, B., Opačić, D., Džudović, B., Crevar, M.,& Gojković-Bukarica, L.. (2022). Antiarrhythmic effects of newly developed propafenone derivatives. in Arhiv za farmaciju
Pharmaceutical Association of Serbia., 72(4), 392-412.
https://doi.org/10.5937/arhfarm72-37114

Ivković B, Opačić D, Džudović B, Crevar M, Gojković-Bukarica L. Antiarrhythmic effects of newly developed propafenone derivatives. in Arhiv za farmaciju. 2022;72(4):392-412.
doi:10.5937/arhfarm72-37114 .

Ivković, Branka, Opačić, Dragan, Džudović, Boris, Crevar, Milkica, Gojković-Bukarica, Ljiljana, "Antiarrhythmic effects of newly developed propafenone derivatives" in Arhiv za farmaciju, 72, no. 4 (2022):392-412,
https://doi.org/10.5937/arhfarm72-37114 . .

TY  - JOUR
AU  - Lisov, Nikolina
AU  - Petrović, Aleksandar
AU  - Čakar, Uroš
AU  - Jadranin, Milka
AU  - Tešević, Vele
AU  - Bukarica-Gojković, Ljiljana
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3767
AB  - In this study, we estimated the usage of Cabernet Sauvignon in microvinifications, obtaining wines with increased phenolic compound content. Kinetic extraction of phenolic compounds during alcoholic fermentation was affected by maceration time (3, 5, 7, 14 and 21 days) and the addition and kinetics of enzyme preparations (EP). The highest extraction rates were observed for catechin (EXV EP – EXV en-zyme preparation and CP EP – Color plus enzyme preparation) and p-hydroxybenzoic acid (Car EP - Ca-ractere enzyme preparation). According to extraction time of the analyzed phenolic compounds, maximal values (ellagic acid, ferulic acid, chlorogenic acid, caffeic acid, naringenin, p-hydroxybenzoic acid, p-coumaric acid, protocatechuic acid, trans-resveratrol, syringic acid, vanillin, and vanillic acid) were ob-tained on day 15 of maceration with addition of CP EP, with exceptions of gallic acid, catechin, and my-ricetin. Prolonged maceration times, up to 21 days, showed the most potent DPPH free radical scavenging activity with Car EP and the highest Ferric Reducing Ability of Plasma (FRAP) values with CP EP.
PB  - Macedonian Journal of Chemistry and Chemical Engineering
T2  - Macedonian Journal of Chemistry and Chemical Engineering
T1  - Extraction Kinetic of Some Phenolic Compounds During Cabernet Sauvignon Alcoholic Fermentation and Antioxidant Properties of Derived Wines
VL  - 39
IS  - 2
SP  - 185
EP  - 196
DO  - 10.20450/mjcce.2020.2060
ER  - 

@article{
author = "Lisov, Nikolina and Petrović, Aleksandar and Čakar, Uroš and Jadranin, Milka and Tešević, Vele and Bukarica-Gojković, Ljiljana",
year = "2020",
abstract = "In this study, we estimated the usage of Cabernet Sauvignon in microvinifications, obtaining wines with increased phenolic compound content. Kinetic extraction of phenolic compounds during alcoholic fermentation was affected by maceration time (3, 5, 7, 14 and 21 days) and the addition and kinetics of enzyme preparations (EP). The highest extraction rates were observed for catechin (EXV EP – EXV en-zyme preparation and CP EP – Color plus enzyme preparation) and p-hydroxybenzoic acid (Car EP - Ca-ractere enzyme preparation). According to extraction time of the analyzed phenolic compounds, maximal values (ellagic acid, ferulic acid, chlorogenic acid, caffeic acid, naringenin, p-hydroxybenzoic acid, p-coumaric acid, protocatechuic acid, trans-resveratrol, syringic acid, vanillin, and vanillic acid) were ob-tained on day 15 of maceration with addition of CP EP, with exceptions of gallic acid, catechin, and my-ricetin. Prolonged maceration times, up to 21 days, showed the most potent DPPH free radical scavenging activity with Car EP and the highest Ferric Reducing Ability of Plasma (FRAP) values with CP EP.",
publisher = "Macedonian Journal of Chemistry and Chemical Engineering",
journal = "Macedonian Journal of Chemistry and Chemical Engineering",
title = "Extraction Kinetic of Some Phenolic Compounds During Cabernet Sauvignon Alcoholic Fermentation and Antioxidant Properties of Derived Wines",
volume = "39",
number = "2",
pages = "185-196",
doi = "10.20450/mjcce.2020.2060"
}

Lisov, N., Petrović, A., Čakar, U., Jadranin, M., Tešević, V.,& Bukarica-Gojković, L.. (2020). Extraction Kinetic of Some Phenolic Compounds During Cabernet Sauvignon Alcoholic Fermentation and Antioxidant Properties of Derived Wines. in Macedonian Journal of Chemistry and Chemical Engineering
Macedonian Journal of Chemistry and Chemical Engineering., 39(2), 185-196.
https://doi.org/10.20450/mjcce.2020.2060

Lisov N, Petrović A, Čakar U, Jadranin M, Tešević V, Bukarica-Gojković L. Extraction Kinetic of Some Phenolic Compounds During Cabernet Sauvignon Alcoholic Fermentation and Antioxidant Properties of Derived Wines. in Macedonian Journal of Chemistry and Chemical Engineering. 2020;39(2):185-196.
doi:10.20450/mjcce.2020.2060 .

Lisov, Nikolina, Petrović, Aleksandar, Čakar, Uroš, Jadranin, Milka, Tešević, Vele, Bukarica-Gojković, Ljiljana, "Extraction Kinetic of Some Phenolic Compounds During Cabernet Sauvignon Alcoholic Fermentation and Antioxidant Properties of Derived Wines" in Macedonian Journal of Chemistry and Chemical Engineering, 39, no. 2 (2020):185-196,
https://doi.org/10.20450/mjcce.2020.2060 . .

TY  - JOUR
AU  - Novaković, Radmila
AU  - Radunović, Nebojša
AU  - Rajković, Jovana
AU  - Đokić, Vladimir
AU  - Petrović, Aleksandar V.
AU  - Ivković, Branka
AU  - Ćupić, Vitomir
AU  - Kanjuh, Vladimir
AU  - Helmut, Heinlev
AU  - Gojković-Bukarica, Ljiljana
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2769
AB  - Resveratrol is a phytoalexin produced in a number of plant species including grapes. The benefit of resveratrol to health is widely reported. Resveratrol has been found to promote relaxation of non-pregnant and pregnant uterus, but its mechanism of action is unclear. The aims of our study were to investigate the involvement of inwardly rectifying potassium channels (Kir) in inhibitory effects of resveratrol on three models of contractions of non-pregnant rat uterus: the spontaneous rhythmic contractions (SRC), oxytocin-elicited phasic contractions and tonic oxytocin-elicited contractions. Uterine strips were obtained from virgin female Wistar rats in oestrus. Strips were mounted into organ bath for recording isometric tension in Krebs-Ringer solution. Experiments followed a multiple curve design. In order to test the involvement of Kirchannels in a mechanism of action of resveratrol (1-100 μM),BaCl2 (1 mM),a antagonist of inwardly rectifying potassium channels was used. Resveratrol induced a concentration-dependent relaxation of all models of contractions. BaCl2 antagonized the response to resveratrolon SRC and oxytocin-elicited phasic contractions. Relaxation achieved by resveratrolon tonic oxytocin-elicited concentrations was insensitive to BaCl2.The antagonism of resveratrol effects by inwardly rectifying potassium channels antagonist suggests that Kir channels are involved in resveratrol action on phasic contractions of rat uterus. Inhibitory effect of resveratrol on tonic contractions did not include Kir channels.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski glasnik
T1  - Wine polyphenol resveratrol inhibits contractions of isolated rat uterus by activation of smooth muscle inwardly rectifying potassium channels
VL  - 70
IS  - 3-4
SP  - 121
EP  - 129
DO  - 10.2298/VETGL1604121N
ER  - 

@article{
author = "Novaković, Radmila and Radunović, Nebojša and Rajković, Jovana and Đokić, Vladimir and Petrović, Aleksandar V. and Ivković, Branka and Ćupić, Vitomir and Kanjuh, Vladimir and Helmut, Heinlev and Gojković-Bukarica, Ljiljana",
year = "2016",
abstract = "Resveratrol is a phytoalexin produced in a number of plant species including grapes. The benefit of resveratrol to health is widely reported. Resveratrol has been found to promote relaxation of non-pregnant and pregnant uterus, but its mechanism of action is unclear. The aims of our study were to investigate the involvement of inwardly rectifying potassium channels (Kir) in inhibitory effects of resveratrol on three models of contractions of non-pregnant rat uterus: the spontaneous rhythmic contractions (SRC), oxytocin-elicited phasic contractions and tonic oxytocin-elicited contractions. Uterine strips were obtained from virgin female Wistar rats in oestrus. Strips were mounted into organ bath for recording isometric tension in Krebs-Ringer solution. Experiments followed a multiple curve design. In order to test the involvement of Kirchannels in a mechanism of action of resveratrol (1-100 μM),BaCl2 (1 mM),a antagonist of inwardly rectifying potassium channels was used. Resveratrol induced a concentration-dependent relaxation of all models of contractions. BaCl2 antagonized the response to resveratrolon SRC and oxytocin-elicited phasic contractions. Relaxation achieved by resveratrolon tonic oxytocin-elicited concentrations was insensitive to BaCl2.The antagonism of resveratrol effects by inwardly rectifying potassium channels antagonist suggests that Kir channels are involved in resveratrol action on phasic contractions of rat uterus. Inhibitory effect of resveratrol on tonic contractions did not include Kir channels.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski glasnik",
title = "Wine polyphenol resveratrol inhibits contractions of isolated rat uterus by activation of smooth muscle inwardly rectifying potassium channels",
volume = "70",
number = "3-4",
pages = "121-129",
doi = "10.2298/VETGL1604121N"
}

Novaković, R., Radunović, N., Rajković, J., Đokić, V., Petrović, A. V., Ivković, B., Ćupić, V., Kanjuh, V., Helmut, H.,& Gojković-Bukarica, L.. (2016). Wine polyphenol resveratrol inhibits contractions of isolated rat uterus by activation of smooth muscle inwardly rectifying potassium channels. in Veterinarski glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 70(3-4), 121-129.
https://doi.org/10.2298/VETGL1604121N

Novaković R, Radunović N, Rajković J, Đokić V, Petrović AV, Ivković B, Ćupić V, Kanjuh V, Helmut H, Gojković-Bukarica L. Wine polyphenol resveratrol inhibits contractions of isolated rat uterus by activation of smooth muscle inwardly rectifying potassium channels. in Veterinarski glasnik. 2016;70(3-4):121-129.
doi:10.2298/VETGL1604121N .

Novaković, Radmila, Radunović, Nebojša, Rajković, Jovana, Đokić, Vladimir, Petrović, Aleksandar V., Ivković, Branka, Ćupić, Vitomir, Kanjuh, Vladimir, Helmut, Heinlev, Gojković-Bukarica, Ljiljana, "Wine polyphenol resveratrol inhibits contractions of isolated rat uterus by activation of smooth muscle inwardly rectifying potassium channels" in Veterinarski glasnik, 70, no. 3-4 (2016):121-129,
https://doi.org/10.2298/VETGL1604121N . .

TY  - JOUR
AU  - Novaković, Radmila
AU  - Radunović, Nebojša
AU  - Marković-Lipkovski, Jasmina
AU  - Cirović, S.
AU  - Beleslin-Cokić, Bojana B.
AU  - Ilić, B.
AU  - Ivković, Branka
AU  - Heinle, Helmut
AU  - Živanović, Vladimir
AU  - Gojković-Bukarica, Ljiljana
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2462
AB  - The ideal agent for prevention and treatment of uterine abnormal contractility has not been found. The polyphenol resveratrol possesses a wide spectrum of pharmacologic properties, but its influence on the contractility of human myometrium is not defined. The present study evaluated the effect of resveratrol on the oxytocin-induced contractions of human term pregnant myometrium in vitro and the contribution of different K+ channels to resveratrol action. Resveratrol induced a concentration-dependent relaxation of myometrium contractions (pD(2) value and maximal responses were 4.52 and 82.25%, respectively). Glibenclamide, a selective blocker of ATP-sensitive (K-ATP), iberiotoxin, a selective blockers of big-calcium sensitive (BKCa) and 4-aminopiridine, a non-selective blocker of voltage-sensitive (Kv) channels induced a significant shift to the right of the concentration-response curves of resveratrol. Inhibition achieved by 0.1 mM resveratrol was insensitive to all K+ channel blockers. A K+ channel opener, pinacidil, inhibited oxytocin-induced contractions of pregnant myometrium with comparable potency and efficacy to resveratrol (pD(2) values and maximal relaxation were 4.52 and 83.67%, respectively). Based on K+ channel opener/blocker affinities, it appears that the inhibitory response of resveratrol involves different myometrial K+ channels. When applied in high concentrations, resveratrol has an additional K+-channel-independent mechanism(s) of action. Furthermore, immunohistochemistry staining and western blot analyses detected the presence and distribution of K-ATP, BKCa and Kv channel proteins in pregnant myometrium.
PB  - Oxford Univ Press, Oxford
T2  - Molecular Human Reproduction
T1  - Effects of the polyphenol resveratrol on contractility of human term pregnant myometrium
VL  - 21
IS  - 6
SP  - 545
EP  - 551
DO  - 10.1093/molehr/gav011
ER  - 

@article{
author = "Novaković, Radmila and Radunović, Nebojša and Marković-Lipkovski, Jasmina and Cirović, S. and Beleslin-Cokić, Bojana B. and Ilić, B. and Ivković, Branka and Heinle, Helmut and Živanović, Vladimir and Gojković-Bukarica, Ljiljana",
year = "2015",
abstract = "The ideal agent for prevention and treatment of uterine abnormal contractility has not been found. The polyphenol resveratrol possesses a wide spectrum of pharmacologic properties, but its influence on the contractility of human myometrium is not defined. The present study evaluated the effect of resveratrol on the oxytocin-induced contractions of human term pregnant myometrium in vitro and the contribution of different K+ channels to resveratrol action. Resveratrol induced a concentration-dependent relaxation of myometrium contractions (pD(2) value and maximal responses were 4.52 and 82.25%, respectively). Glibenclamide, a selective blocker of ATP-sensitive (K-ATP), iberiotoxin, a selective blockers of big-calcium sensitive (BKCa) and 4-aminopiridine, a non-selective blocker of voltage-sensitive (Kv) channels induced a significant shift to the right of the concentration-response curves of resveratrol. Inhibition achieved by 0.1 mM resveratrol was insensitive to all K+ channel blockers. A K+ channel opener, pinacidil, inhibited oxytocin-induced contractions of pregnant myometrium with comparable potency and efficacy to resveratrol (pD(2) values and maximal relaxation were 4.52 and 83.67%, respectively). Based on K+ channel opener/blocker affinities, it appears that the inhibitory response of resveratrol involves different myometrial K+ channels. When applied in high concentrations, resveratrol has an additional K+-channel-independent mechanism(s) of action. Furthermore, immunohistochemistry staining and western blot analyses detected the presence and distribution of K-ATP, BKCa and Kv channel proteins in pregnant myometrium.",
publisher = "Oxford Univ Press, Oxford",
journal = "Molecular Human Reproduction",
title = "Effects of the polyphenol resveratrol on contractility of human term pregnant myometrium",
volume = "21",
number = "6",
pages = "545-551",
doi = "10.1093/molehr/gav011"
}

Novaković, R., Radunović, N., Marković-Lipkovski, J., Cirović, S., Beleslin-Cokić, B. B., Ilić, B., Ivković, B., Heinle, H., Živanović, V.,& Gojković-Bukarica, L.. (2015). Effects of the polyphenol resveratrol on contractility of human term pregnant myometrium. in Molecular Human Reproduction
Oxford Univ Press, Oxford., 21(6), 545-551.
https://doi.org/10.1093/molehr/gav011

Novaković R, Radunović N, Marković-Lipkovski J, Cirović S, Beleslin-Cokić BB, Ilić B, Ivković B, Heinle H, Živanović V, Gojković-Bukarica L. Effects of the polyphenol resveratrol on contractility of human term pregnant myometrium. in Molecular Human Reproduction. 2015;21(6):545-551.
doi:10.1093/molehr/gav011 .

Novaković, Radmila, Radunović, Nebojša, Marković-Lipkovski, Jasmina, Cirović, S., Beleslin-Cokić, Bojana B., Ilić, B., Ivković, Branka, Heinle, Helmut, Živanović, Vladimir, Gojković-Bukarica, Ljiljana, "Effects of the polyphenol resveratrol on contractility of human term pregnant myometrium" in Molecular Human Reproduction, 21, no. 6 (2015):545-551,
https://doi.org/10.1093/molehr/gav011 . .

TY  - JOUR
AU  - Ivković, Branka
AU  - Gojković-Bukarica, Ljiljana
AU  - Novaković, Radmila
AU  - Ćupić, Vitomir
AU  - Vladimirov, Sote
AU  - Živanović, Vladimir
AU  - Šćepanović, Radisav
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2297
AB  - By applying of aconitictest in in vivo experiments in rats under deep anesthesia, there was investigated the antiarrhythmic potential of newly synthetized fluorinated derivatives of propafenone. The animals were divided into four experimental groups. The first (aconitine group) was treated with aconitine at a dose of 60 μg/kg, which led to pronounced cardiac rhythm disorder in a short period of time. The appearance of ventricular extrasystole (VES) was taken as a parameter for ascertainment of cardiac rhytm disorder. The remaining three animal groups were taken for testing the potential of propafenone and propafenone fluorinated derivatives to stop the arrhythmia, and which was induced by i.v. aconitine injection (60 μg/kg). Propafenone, as well as 50F derivative, did not convert the disturbed cardiac rhythm (survival of animals was 0%). By applying 5PF derivative in a dose of 6 mg/kg, the animals survived with occasional establishment of sinus rhythm.
AB  - Primenom akonitinskog testa, u in vivo eksperimentima na pacovima u dubokoj anesteziji, ispitivan je antiaritmijski potencijal novosintetisanih fluoriranih derivata propafenona. Životinje su podeljene u četiri eksperimentalne grupe. Prva grupa (akonitinska grupa) je tretirana akonitinom u dozi od 60 μg/kg t.m., koja dovodi do vidnog poremećaja srčanog ritma u kratkom vremenskom periodu. Kao parameter za registrovanje poremećaja srčanog ritma uzetaje pojava ventrikularne ekstrasistole (VES). Ostale (tri) eksperimentalne grupe činile su životinje na kojima je ispitivan potencijal propafenona i fluoriranih derivata propafenona da zaustave aritmiju indukovanu i.v. injekcijom akonitina (60 μg/kg t.m.). Propafenon, kao i 50F derivat, nisu uspeli da konvertuju poremećen srčani ritam (preživljavanje životinja je 0 %). Prilikom aplikacije 5PF derivata u dozi od 6 mg/kg t.m. životinje su preživele, uz povremeno uspostavljanje sinusnog ritma.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski glasnik
T1  - Testing of newly synthesized propafenone derivatives antiarrhythmic activity in aconitic model of cardiac arrhythmia in rats
T1  - Issledovanie antiaritmičeskoj aktivnosti vnov' sintezirovannyh proizvodnyh propafenona pri modelirovanii akonitovoi aritmii serdca u krys
T1  - Ispitivanje antiaritmijske aktivnosti novosintetisanih derivata propafenona u akonitinskom modelu srčane aritmije kod pacova
VL  - 68
IS  - 5-6
SP  - 281
EP  - 290
DO  - 10.2298/VETGL1406281I
ER  - 

@article{
author = "Ivković, Branka and Gojković-Bukarica, Ljiljana and Novaković, Radmila and Ćupić, Vitomir and Vladimirov, Sote and Živanović, Vladimir and Šćepanović, Radisav",
year = "2014",
abstract = "By applying of aconitictest in in vivo experiments in rats under deep anesthesia, there was investigated the antiarrhythmic potential of newly synthetized fluorinated derivatives of propafenone. The animals were divided into four experimental groups. The first (aconitine group) was treated with aconitine at a dose of 60 μg/kg, which led to pronounced cardiac rhythm disorder in a short period of time. The appearance of ventricular extrasystole (VES) was taken as a parameter for ascertainment of cardiac rhytm disorder. The remaining three animal groups were taken for testing the potential of propafenone and propafenone fluorinated derivatives to stop the arrhythmia, and which was induced by i.v. aconitine injection (60 μg/kg). Propafenone, as well as 50F derivative, did not convert the disturbed cardiac rhythm (survival of animals was 0%). By applying 5PF derivative in a dose of 6 mg/kg, the animals survived with occasional establishment of sinus rhythm., Primenom akonitinskog testa, u in vivo eksperimentima na pacovima u dubokoj anesteziji, ispitivan je antiaritmijski potencijal novosintetisanih fluoriranih derivata propafenona. Životinje su podeljene u četiri eksperimentalne grupe. Prva grupa (akonitinska grupa) je tretirana akonitinom u dozi od 60 μg/kg t.m., koja dovodi do vidnog poremećaja srčanog ritma u kratkom vremenskom periodu. Kao parameter za registrovanje poremećaja srčanog ritma uzetaje pojava ventrikularne ekstrasistole (VES). Ostale (tri) eksperimentalne grupe činile su životinje na kojima je ispitivan potencijal propafenona i fluoriranih derivata propafenona da zaustave aritmiju indukovanu i.v. injekcijom akonitina (60 μg/kg t.m.). Propafenon, kao i 50F derivat, nisu uspeli da konvertuju poremećen srčani ritam (preživljavanje životinja je 0 %). Prilikom aplikacije 5PF derivata u dozi od 6 mg/kg t.m. životinje su preživele, uz povremeno uspostavljanje sinusnog ritma.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski glasnik",
title = "Testing of newly synthesized propafenone derivatives antiarrhythmic activity in aconitic model of cardiac arrhythmia in rats, Issledovanie antiaritmičeskoj aktivnosti vnov' sintezirovannyh proizvodnyh propafenona pri modelirovanii akonitovoi aritmii serdca u krys, Ispitivanje antiaritmijske aktivnosti novosintetisanih derivata propafenona u akonitinskom modelu srčane aritmije kod pacova",
volume = "68",
number = "5-6",
pages = "281-290",
doi = "10.2298/VETGL1406281I"
}

Ivković, B., Gojković-Bukarica, L., Novaković, R., Ćupić, V., Vladimirov, S., Živanović, V.,& Šćepanović, R.. (2014). Testing of newly synthesized propafenone derivatives antiarrhythmic activity in aconitic model of cardiac arrhythmia in rats. in Veterinarski glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 68(5-6), 281-290.
https://doi.org/10.2298/VETGL1406281I

Ivković B, Gojković-Bukarica L, Novaković R, Ćupić V, Vladimirov S, Živanović V, Šćepanović R. Testing of newly synthesized propafenone derivatives antiarrhythmic activity in aconitic model of cardiac arrhythmia in rats. in Veterinarski glasnik. 2014;68(5-6):281-290.
doi:10.2298/VETGL1406281I .

Ivković, Branka, Gojković-Bukarica, Ljiljana, Novaković, Radmila, Ćupić, Vitomir, Vladimirov, Sote, Živanović, Vladimir, Šćepanović, Radisav, "Testing of newly synthesized propafenone derivatives antiarrhythmic activity in aconitic model of cardiac arrhythmia in rats" in Veterinarski glasnik, 68, no. 5-6 (2014):281-290,
https://doi.org/10.2298/VETGL1406281I . .

TY  - JOUR
AU  - Gojković-Bukarica, Ljiljana
AU  - Beleslin-Cokić, Bojana B.
AU  - Novaković, Aleksandra
AU  - Perić, Miodrag
AU  - Marković-Lipkovski, Jasmina
AU  - Cirović, Sanja Z.
AU  - Nezić, Dušan
AU  - Lesić, Aleksandar R.
AU  - Kanjuh, Vladimir
AU  - Heinle, Helmut
PY  - 2011
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1571
AB  - Because adrenergic contractions can contribute to the development of life-threatening spasm of coronary artery bypass graft, this study was performed to investigate the effect of adenosine 3-phosphate (ATP)-sensitive K(+) channel (K(ATP)) opener P1075 on contractions of isolated human saphenous vein (HSV) and human internal mammary artery (HIMA). Phasic contractions were evoked by electric field stimulation (20 Hz) and noradrenaline. The sustained contractions were evoked by phenylephrine. The presence of pore-forming Kir6.1 and Kir6.2 subunits of the K(ATP) channels in the HIMA and only Kir6.2 in the HSV was confirmed immunomorphologically. P1075 inhibited in the HSV only, the electrical field stimulation contractions more strongly than noradrenaline contractions. In addition, the phenylephrine contractions of HSV were more sensitive to P1075 in comparison to those of HIMA. Glibenclamide, a K(ATP) channel blocker antagonized the vasodilatation produced by P1075 in both grafts differently, because its effect was more prominent on the P1075-induced inhibition of contractions of HSV than of HIMA. We conclude that P1075 has a vasorelaxant effect and inhibited adrenergic contractions of the tested grafts. This effect is graft and vasoconstrictor selective and seems to be mediated by Kir6.1-and/or Kir6.2-containing K(ATP) channels. Thus, P1075 can be considered as a potential drug in the prevention of graft spasm.
PB  - Lippincott Williams & Wilkins, Philadelphia
T2  - Journal of Cardiovascular Pharmacology
T1  - The Effects of Potassium Channel Opener P1075 on the Human Saphenous Vein and Human Internal Mammary Artery
VL  - 57
IS  - 6
SP  - 648
EP  - 655
DO  - 10.1097/FJC.0b013e3182145850
ER  - 

@article{
author = "Gojković-Bukarica, Ljiljana and Beleslin-Cokić, Bojana B. and Novaković, Aleksandra and Perić, Miodrag and Marković-Lipkovski, Jasmina and Cirović, Sanja Z. and Nezić, Dušan and Lesić, Aleksandar R. and Kanjuh, Vladimir and Heinle, Helmut",
year = "2011",
abstract = "Because adrenergic contractions can contribute to the development of life-threatening spasm of coronary artery bypass graft, this study was performed to investigate the effect of adenosine 3-phosphate (ATP)-sensitive K(+) channel (K(ATP)) opener P1075 on contractions of isolated human saphenous vein (HSV) and human internal mammary artery (HIMA). Phasic contractions were evoked by electric field stimulation (20 Hz) and noradrenaline. The sustained contractions were evoked by phenylephrine. The presence of pore-forming Kir6.1 and Kir6.2 subunits of the K(ATP) channels in the HIMA and only Kir6.2 in the HSV was confirmed immunomorphologically. P1075 inhibited in the HSV only, the electrical field stimulation contractions more strongly than noradrenaline contractions. In addition, the phenylephrine contractions of HSV were more sensitive to P1075 in comparison to those of HIMA. Glibenclamide, a K(ATP) channel blocker antagonized the vasodilatation produced by P1075 in both grafts differently, because its effect was more prominent on the P1075-induced inhibition of contractions of HSV than of HIMA. We conclude that P1075 has a vasorelaxant effect and inhibited adrenergic contractions of the tested grafts. This effect is graft and vasoconstrictor selective and seems to be mediated by Kir6.1-and/or Kir6.2-containing K(ATP) channels. Thus, P1075 can be considered as a potential drug in the prevention of graft spasm.",
publisher = "Lippincott Williams & Wilkins, Philadelphia",
journal = "Journal of Cardiovascular Pharmacology",
title = "The Effects of Potassium Channel Opener P1075 on the Human Saphenous Vein and Human Internal Mammary Artery",
volume = "57",
number = "6",
pages = "648-655",
doi = "10.1097/FJC.0b013e3182145850"
}

Gojković-Bukarica, L., Beleslin-Cokić, B. B., Novaković, A., Perić, M., Marković-Lipkovski, J., Cirović, S. Z., Nezić, D., Lesić, A. R., Kanjuh, V.,& Heinle, H.. (2011). The Effects of Potassium Channel Opener P1075 on the Human Saphenous Vein and Human Internal Mammary Artery. in Journal of Cardiovascular Pharmacology
Lippincott Williams & Wilkins, Philadelphia., 57(6), 648-655.
https://doi.org/10.1097/FJC.0b013e3182145850

Gojković-Bukarica L, Beleslin-Cokić BB, Novaković A, Perić M, Marković-Lipkovski J, Cirović SZ, Nezić D, Lesić AR, Kanjuh V, Heinle H. The Effects of Potassium Channel Opener P1075 on the Human Saphenous Vein and Human Internal Mammary Artery. in Journal of Cardiovascular Pharmacology. 2011;57(6):648-655.
doi:10.1097/FJC.0b013e3182145850 .

Gojković-Bukarica, Ljiljana, Beleslin-Cokić, Bojana B., Novaković, Aleksandra, Perić, Miodrag, Marković-Lipkovski, Jasmina, Cirović, Sanja Z., Nezić, Dušan, Lesić, Aleksandar R., Kanjuh, Vladimir, Heinle, Helmut, "The Effects of Potassium Channel Opener P1075 on the Human Saphenous Vein and Human Internal Mammary Artery" in Journal of Cardiovascular Pharmacology, 57, no. 6 (2011):648-655,
https://doi.org/10.1097/FJC.0b013e3182145850 . .