TY  - JOUR
AU  - Žugić, Ana
AU  - Tadić, Vanja
AU  - Savić, Snežana
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3545
AB  - Usnic acid is one of the most investigated lichen secondary metabolites, with several proven biological properties with potential medical relevance. However, its unfavorable physico-chemical properties, as well as observed hepatotoxicity, have discouraged wide-range utilization of usnic acid as a promising therapeutic agent. In accordance with the growing research interest in the development of nanotechnology, especially in the arena of preparations based on natural sources of medicinal compounds, usnic acid incorporated into nano-and microsized colloidal carriers has been a subject of a large number of publications. Therefore, this review discusses the overall results of the studies dealing with usnic acid encapsulated into lipid-based, polymeric and nonorganic micro-and/or nanocarriers, as potential drug delivery systems for this natural compound, in an attempt to introduce its usage as a potential antitumor, antimicrobial, wound-healing, antioxidative and anti-inflammatory drug.
PB  - MDPI
T2  - Pharmaceutics
T1  - Nano-and microcarriers as drug delivery systems for usnic acid: Review of literature
VL  - 12
IS  - 2
DO  - 10.3390/pharmaceutics12020156
ER  - 

@article{
author = "Žugić, Ana and Tadić, Vanja and Savić, Snežana",
year = "2020",
abstract = "Usnic acid is one of the most investigated lichen secondary metabolites, with several proven biological properties with potential medical relevance. However, its unfavorable physico-chemical properties, as well as observed hepatotoxicity, have discouraged wide-range utilization of usnic acid as a promising therapeutic agent. In accordance with the growing research interest in the development of nanotechnology, especially in the arena of preparations based on natural sources of medicinal compounds, usnic acid incorporated into nano-and microsized colloidal carriers has been a subject of a large number of publications. Therefore, this review discusses the overall results of the studies dealing with usnic acid encapsulated into lipid-based, polymeric and nonorganic micro-and/or nanocarriers, as potential drug delivery systems for this natural compound, in an attempt to introduce its usage as a potential antitumor, antimicrobial, wound-healing, antioxidative and anti-inflammatory drug.",
publisher = "MDPI",
journal = "Pharmaceutics",
title = "Nano-and microcarriers as drug delivery systems for usnic acid: Review of literature",
volume = "12",
number = "2",
doi = "10.3390/pharmaceutics12020156"
}

Žugić, A., Tadić, V.,& Savić, S.. (2020). Nano-and microcarriers as drug delivery systems for usnic acid: Review of literature. in Pharmaceutics
MDPI., 12(2).
https://doi.org/10.3390/pharmaceutics12020156

Žugić A, Tadić V, Savić S. Nano-and microcarriers as drug delivery systems for usnic acid: Review of literature. in Pharmaceutics. 2020;12(2).
doi:10.3390/pharmaceutics12020156 .

Žugić, Ana, Tadić, Vanja, Savić, Snežana, "Nano-and microcarriers as drug delivery systems for usnic acid: Review of literature" in Pharmaceutics, 12, no. 2 (2020),
https://doi.org/10.3390/pharmaceutics12020156 . .

TY  - JOUR
AU  - Žugić, Ana
AU  - Isaković, Aleksandra
AU  - Jeremić, Ivica
AU  - Savić, Snežana
AU  - Tadić, Vanja
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4664
AB  - Old man’s beard (Usnea barbata) is one of the most investigated lichens which biological properties
(such as antimicrobial, anti-inflammatory and cytotoxic activities) have been proven in several scien-
tific studies. Stated properties of old man’s beard have been attributed mainly to its major secondary
metabolite - a dibenzofuran derivative, usnic acid. The aim of the current study was to examine cyto-
toxic activity of the quantified supercritical CO2 extract and pure usnic acid in parallel against L929
fibrosarcoma cells. Our results indicated the tested extract to be more toxic towards the investigated
cell line compared to usnic acid per se, despite the high amount of this compound (81.41% (w/w))
revealed in the extract using HPLC. Such result may be connected to the unidentified compounds
present in the supercritical CO2 extract that could be responsible for apoptosis and oxidative stress in
the exerted cytotoxicity of the investigated extract, but not usnic acid. Our study supports further in-
vestigations of supercritical CO2 extract of U. barbata as a prospective therapeutic agent with potential
relevance in the treatment of sarcoma.
PB  - Institut za proučavanje lekovitog bilja "Dr Josif Pančić", Beograd
T2  - Lekovite sirovine
T1  - Cytotoxic activity of supercritical CO2 extract of old man's beard in L929 fibrosarcoma cell line
VL  - 39
SP  - 30
EP  - 34
DO  - 10.5937/leksir1939030Z
ER  - 

@article{
author = "Žugić, Ana and Isaković, Aleksandra and Jeremić, Ivica and Savić, Snežana and Tadić, Vanja",
year = "2019",
abstract = "Old man’s beard (Usnea barbata) is one of the most investigated lichens which biological properties
(such as antimicrobial, anti-inflammatory and cytotoxic activities) have been proven in several scien-
tific studies. Stated properties of old man’s beard have been attributed mainly to its major secondary
metabolite - a dibenzofuran derivative, usnic acid. The aim of the current study was to examine cyto-
toxic activity of the quantified supercritical CO2 extract and pure usnic acid in parallel against L929
fibrosarcoma cells. Our results indicated the tested extract to be more toxic towards the investigated
cell line compared to usnic acid per se, despite the high amount of this compound (81.41% (w/w))
revealed in the extract using HPLC. Such result may be connected to the unidentified compounds
present in the supercritical CO2 extract that could be responsible for apoptosis and oxidative stress in
the exerted cytotoxicity of the investigated extract, but not usnic acid. Our study supports further in-
vestigations of supercritical CO2 extract of U. barbata as a prospective therapeutic agent with potential
relevance in the treatment of sarcoma.",
publisher = "Institut za proučavanje lekovitog bilja "Dr Josif Pančić", Beograd",
journal = "Lekovite sirovine",
title = "Cytotoxic activity of supercritical CO2 extract of old man's beard in L929 fibrosarcoma cell line",
volume = "39",
pages = "30-34",
doi = "10.5937/leksir1939030Z"
}

Žugić, A., Isaković, A., Jeremić, I., Savić, S.,& Tadić, V.. (2019). Cytotoxic activity of supercritical CO2 extract of old man's beard in L929 fibrosarcoma cell line. in Lekovite sirovine
Institut za proučavanje lekovitog bilja "Dr Josif Pančić", Beograd., 39, 30-34.
https://doi.org/10.5937/leksir1939030Z

Žugić A, Isaković A, Jeremić I, Savić S, Tadić V. Cytotoxic activity of supercritical CO2 extract of old man's beard in L929 fibrosarcoma cell line. in Lekovite sirovine. 2019;39:30-34.
doi:10.5937/leksir1939030Z .

Žugić, Ana, Isaković, Aleksandra, Jeremić, Ivica, Savić, Snežana, Tadić, Vanja, "Cytotoxic activity of supercritical CO2 extract of old man's beard in L929 fibrosarcoma cell line" in Lekovite sirovine, 39 (2019):30-34,
https://doi.org/10.5937/leksir1939030Z . .

TY  - CONF
AU  - Đuriš, Jelena
AU  - Milovanović, Stoja
AU  - Medarević, Đorđe
AU  - Dobričić, Vladimir
AU  - Ibrić, Svetlana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5483
AB  - Formulation of solid dispersions is one of the most feasible strategies for overcoming the poor
drugs’ solubility – one of the major issues affecting drug bioavailability and therapeutic outcomes.
Since traditional methods used for preparation of solid dispersions often require usage of organic
solvents, it is of great importance to seek for more environment-friendly methods. It has been
demonstrated that supercritical (sc) CO2 may be effectively utilized for dispersion of drugs into the
suitable carrier (polymer), thus obtaining solid dispersions with the improved drug dissolution rate.
The aim of the presented study was to investigate the potential of scCO2 for preparation of poorly
soluble antihypertensive drugs (carvedilol and valsartan) solid dispersions in the conventional
pharmaceutical polymers (polyvinilypyrolidone-PVP and hypromellose-HPMC). Prepared solid
dispersions were characterized by scanning electron microscopy, differential scanning calorimetry
and Fourier-transform infrared spectroscopy; their porosity and density were determined and drug
dissolution rate was assessed and compared to the results obtained from solid dispersions prepared
by the traditional solvent casting method. Selected samples were tested for their tableting properties
as well. It has been demonstrated that scCO2 may be successfully applied for preparation of
carvedilol or valsartan solid dispersions with the improved drug dissolution rate. Further
characterization revealed the nature of interactions between the drugs and selected polymers. Due
to the low density (and high porosity), some of the prepared solid dispersions may be used for
further development of the floating dosage forms. It has also been demonstrated that some of the
prepared dispersions have excellent compressibility and compactibility, which is of great
importance for further development of solid dosage forms. The obtained results provide framework
for further development of environment-friendly methods in pharmaceutical development and
production.
PB  - Materials Research Society of Serbia
PB  - International Institute for the Science of Sintering
C3  - Twenty-first Annual Conference YUCOMAT 2019 & Eleventh World Round Table Conference on Sintering WRTCS 2019, September 2-6, 2019, Herceg Novi, Montenegro
T1  - Supercritical CO2 utilization in preparation of poorly soluble drugs solid dispersions
SP  - 71
EP  - 71
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5483
ER  - 

@conference{
author = "Đuriš, Jelena and Milovanović, Stoja and Medarević, Đorđe and Dobričić, Vladimir and Ibrić, Svetlana",
year = "2019",
abstract = "Formulation of solid dispersions is one of the most feasible strategies for overcoming the poor
drugs’ solubility – one of the major issues affecting drug bioavailability and therapeutic outcomes.
Since traditional methods used for preparation of solid dispersions often require usage of organic
solvents, it is of great importance to seek for more environment-friendly methods. It has been
demonstrated that supercritical (sc) CO2 may be effectively utilized for dispersion of drugs into the
suitable carrier (polymer), thus obtaining solid dispersions with the improved drug dissolution rate.
The aim of the presented study was to investigate the potential of scCO2 for preparation of poorly
soluble antihypertensive drugs (carvedilol and valsartan) solid dispersions in the conventional
pharmaceutical polymers (polyvinilypyrolidone-PVP and hypromellose-HPMC). Prepared solid
dispersions were characterized by scanning electron microscopy, differential scanning calorimetry
and Fourier-transform infrared spectroscopy; their porosity and density were determined and drug
dissolution rate was assessed and compared to the results obtained from solid dispersions prepared
by the traditional solvent casting method. Selected samples were tested for their tableting properties
as well. It has been demonstrated that scCO2 may be successfully applied for preparation of
carvedilol or valsartan solid dispersions with the improved drug dissolution rate. Further
characterization revealed the nature of interactions between the drugs and selected polymers. Due
to the low density (and high porosity), some of the prepared solid dispersions may be used for
further development of the floating dosage forms. It has also been demonstrated that some of the
prepared dispersions have excellent compressibility and compactibility, which is of great
importance for further development of solid dosage forms. The obtained results provide framework
for further development of environment-friendly methods in pharmaceutical development and
production.",
publisher = "Materials Research Society of Serbia, International Institute for the Science of Sintering",
journal = "Twenty-first Annual Conference YUCOMAT 2019 & Eleventh World Round Table Conference on Sintering WRTCS 2019, September 2-6, 2019, Herceg Novi, Montenegro",
title = "Supercritical CO2 utilization in preparation of poorly soluble drugs solid dispersions",
pages = "71-71",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5483"
}

Đuriš, J., Milovanović, S., Medarević, Đ., Dobričić, V.,& Ibrić, S.. (2019). Supercritical CO2 utilization in preparation of poorly soluble drugs solid dispersions. in Twenty-first Annual Conference YUCOMAT 2019 & Eleventh World Round Table Conference on Sintering WRTCS 2019, September 2-6, 2019, Herceg Novi, Montenegro
Materials Research Society of Serbia., 71-71.
https://hdl.handle.net/21.15107/rcub_farfar_5483

Đuriš J, Milovanović S, Medarević Đ, Dobričić V, Ibrić S. Supercritical CO2 utilization in preparation of poorly soluble drugs solid dispersions. in Twenty-first Annual Conference YUCOMAT 2019 & Eleventh World Round Table Conference on Sintering WRTCS 2019, September 2-6, 2019, Herceg Novi, Montenegro. 2019;:71-71.
https://hdl.handle.net/21.15107/rcub_farfar_5483 .

Đuriš, Jelena, Milovanović, Stoja, Medarević, Đorđe, Dobričić, Vladimir, Ibrić, Svetlana, "Supercritical CO2 utilization in preparation of poorly soluble drugs solid dispersions" in Twenty-first Annual Conference YUCOMAT 2019 & Eleventh World Round Table Conference on Sintering WRTCS 2019, September 2-6, 2019, Herceg Novi, Montenegro (2019):71-71,
https://hdl.handle.net/21.15107/rcub_farfar_5483 .

TY  - JOUR
AU  - Nesić, I.
AU  - Stojiljković, D.
AU  - Savić, Snežana
AU  - Tasić-Kostov, Marija
AU  - Tadić, Vanja M.
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3246
AB  - Objective The aim of the study was in vitro and in vivo characterization of cosmetic cream with 6% of standardized wild apple fruit extract, stabilized by conventional non-ionic emulsifier-CEW, in order to determine the influence of emulsifiers (conventional vs. biodegradable) on the characteristics of creams and their effects on the skin. Methods Organoleptic and physico-chemical (pH values and electrical conductivity) analysis was performed, determination of fruit acids-FAs content (using HPLC analysis) and estimation of its antioxidant activity-AA (using DPPH test) during 180 days. In vivo study included following examinations: screening of safety profile (after creams application under occlusion during 24 h at human skin); skin moisturizing potential, transepidermal water loss-TEWL, skin pH after 28 days of cream application and hypopigmentation efficacy 7 days of cream application at artificially induced skin hyperpigmentation. Results Investigated cosmetic cream-CEW showed satisfactory organoleptic, physico-chemical characteristics, stability, FAs content (0.13%) and AA (19.25 +/- 0.67 %RSC) after preparation, which remained unchanged over the study period. In vivo investigation revealed absence of skin irritation after CEW's application under occlusion. An increase of skin moisturization (after 14 days Delta EC was 18.52 +/- 11.51 and after 28 days of applications 16.52 +/- 9.36) during 28 day-study, with unchanged TEWL and skin pH values was shown. Decrease of melanin index was revealed, too (after 7 days Delta MI was -31.40 +/- 16.50). Conclusion Cosmetic cream stabilized by conventional emulsifier showed better antioxidant potential and weaker moisturizing and hypopigmentation effects related to the cream with same composition but stabilized by biodegradable emulsifiers. Based on all mentioned above, investigated cosmetic cream might be considered for potential use as modern, stable, safe and efficient cosmetic product in the prevention and/or treatment of oxidative stress-related skin changes and/or damages, for moisturization of dry, even irritated skin as well as for lightening of hyperpigmented skin.
PB  - Wiley, Hoboken
T2  - International Journal of Cosmetic Science
T1  - Stability, antioxidant activity, in vivo safety and efficacy of creams with standardized wild apple fruit extract: a comparison of conventional and biodegradable emulsifiers
VL  - 41
IS  - 3
SP  - 300
EP  - 310
DO  - 10.1111/ics.12537
ER  - 

@article{
author = "Nesić, I. and Stojiljković, D. and Savić, Snežana and Tasić-Kostov, Marija and Tadić, Vanja M.",
year = "2019",
abstract = "Objective The aim of the study was in vitro and in vivo characterization of cosmetic cream with 6% of standardized wild apple fruit extract, stabilized by conventional non-ionic emulsifier-CEW, in order to determine the influence of emulsifiers (conventional vs. biodegradable) on the characteristics of creams and their effects on the skin. Methods Organoleptic and physico-chemical (pH values and electrical conductivity) analysis was performed, determination of fruit acids-FAs content (using HPLC analysis) and estimation of its antioxidant activity-AA (using DPPH test) during 180 days. In vivo study included following examinations: screening of safety profile (after creams application under occlusion during 24 h at human skin); skin moisturizing potential, transepidermal water loss-TEWL, skin pH after 28 days of cream application and hypopigmentation efficacy 7 days of cream application at artificially induced skin hyperpigmentation. Results Investigated cosmetic cream-CEW showed satisfactory organoleptic, physico-chemical characteristics, stability, FAs content (0.13%) and AA (19.25 +/- 0.67 %RSC) after preparation, which remained unchanged over the study period. In vivo investigation revealed absence of skin irritation after CEW's application under occlusion. An increase of skin moisturization (after 14 days Delta EC was 18.52 +/- 11.51 and after 28 days of applications 16.52 +/- 9.36) during 28 day-study, with unchanged TEWL and skin pH values was shown. Decrease of melanin index was revealed, too (after 7 days Delta MI was -31.40 +/- 16.50). Conclusion Cosmetic cream stabilized by conventional emulsifier showed better antioxidant potential and weaker moisturizing and hypopigmentation effects related to the cream with same composition but stabilized by biodegradable emulsifiers. Based on all mentioned above, investigated cosmetic cream might be considered for potential use as modern, stable, safe and efficient cosmetic product in the prevention and/or treatment of oxidative stress-related skin changes and/or damages, for moisturization of dry, even irritated skin as well as for lightening of hyperpigmented skin.",
publisher = "Wiley, Hoboken",
journal = "International Journal of Cosmetic Science",
title = "Stability, antioxidant activity, in vivo safety and efficacy of creams with standardized wild apple fruit extract: a comparison of conventional and biodegradable emulsifiers",
volume = "41",
number = "3",
pages = "300-310",
doi = "10.1111/ics.12537"
}

Nesić, I., Stojiljković, D., Savić, S., Tasić-Kostov, M.,& Tadić, V. M.. (2019). Stability, antioxidant activity, in vivo safety and efficacy of creams with standardized wild apple fruit extract: a comparison of conventional and biodegradable emulsifiers. in International Journal of Cosmetic Science
Wiley, Hoboken., 41(3), 300-310.
https://doi.org/10.1111/ics.12537

Nesić I, Stojiljković D, Savić S, Tasić-Kostov M, Tadić VM. Stability, antioxidant activity, in vivo safety and efficacy of creams with standardized wild apple fruit extract: a comparison of conventional and biodegradable emulsifiers. in International Journal of Cosmetic Science. 2019;41(3):300-310.
doi:10.1111/ics.12537 .

Nesić, I., Stojiljković, D., Savić, Snežana, Tasić-Kostov, Marija, Tadić, Vanja M., "Stability, antioxidant activity, in vivo safety and efficacy of creams with standardized wild apple fruit extract: a comparison of conventional and biodegradable emulsifiers" in International Journal of Cosmetic Science, 41, no. 3 (2019):300-310,
https://doi.org/10.1111/ics.12537 . .

TY  - JOUR
AU  - Milovanović, S
AU  - Đuriš, Jelena
AU  - Dapcević, Aleksandra
AU  - Medarević, Đorđe
AU  - Ibrić, Svetlana
AU  - Zizović, I
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3252
AB  - The present work is aimed towards designing advanced materials by means of sustainable processes. In that sense, the utilization of supercritical CO 2 (scCO 2 ) for processing of pharmaceutical polymers (Soluplus ® , Eudragit ® , and hydroxypropyl methylcellulose acetate succinate), alone and with an addition of cardiovascular drug Carvedilol, was explored. Employed single-step static scCO 2 process (pressure of 30 MPa and temperature of 100 °C for 2 h) enabled fabrication of solvent-free polymeric foams and Carvedilol solid dispersions with controlled microstructure and average pore diameter of 101–257 μm suitable for application in the pharmaceutical industry. ScCO 2 did not remain in the foams after processing or affected the polymer composition, while Carvedilol formed hydrogen bonds with the polymers. Carvedilol was molecularly dispersed in the fabricated solid dispersions and its transition from the crystalline to amorphous form was complete. Korsmeyer-Peppas model was successfully used for the mathematical description of Carvedilol dissolution from solid dispersions. The dissolution rate of Carvedilol in acidic medium was significantly enhanced by its dispersion in tested polymers using the proposed high-pressure method.
PB  - Elsevier Ltd
T2  - Polymer Testing
T1  - Soluplus ® , Eudragit ® , HPMC-AS foams and solid dispersions for enhancement of Carvedilol dissolution rate prepared by a supercritical CO 2 process
VL  - 76
SP  - 54
EP  - 64
DO  - 10.1016/j.polymertesting.2019.03.001
ER  - 

@article{
author = "Milovanović, S and Đuriš, Jelena and Dapcević, Aleksandra and Medarević, Đorđe and Ibrić, Svetlana and Zizović, I",
year = "2019",
abstract = "The present work is aimed towards designing advanced materials by means of sustainable processes. In that sense, the utilization of supercritical CO 2 (scCO 2 ) for processing of pharmaceutical polymers (Soluplus ® , Eudragit ® , and hydroxypropyl methylcellulose acetate succinate), alone and with an addition of cardiovascular drug Carvedilol, was explored. Employed single-step static scCO 2 process (pressure of 30 MPa and temperature of 100 °C for 2 h) enabled fabrication of solvent-free polymeric foams and Carvedilol solid dispersions with controlled microstructure and average pore diameter of 101–257 μm suitable for application in the pharmaceutical industry. ScCO 2 did not remain in the foams after processing or affected the polymer composition, while Carvedilol formed hydrogen bonds with the polymers. Carvedilol was molecularly dispersed in the fabricated solid dispersions and its transition from the crystalline to amorphous form was complete. Korsmeyer-Peppas model was successfully used for the mathematical description of Carvedilol dissolution from solid dispersions. The dissolution rate of Carvedilol in acidic medium was significantly enhanced by its dispersion in tested polymers using the proposed high-pressure method.",
publisher = "Elsevier Ltd",
journal = "Polymer Testing",
title = "Soluplus ® , Eudragit ® , HPMC-AS foams and solid dispersions for enhancement of Carvedilol dissolution rate prepared by a supercritical CO 2 process",
volume = "76",
pages = "54-64",
doi = "10.1016/j.polymertesting.2019.03.001"
}

Milovanović, S., Đuriš, J., Dapcević, A., Medarević, Đ., Ibrić, S.,& Zizović, I.. (2019). Soluplus ® , Eudragit ® , HPMC-AS foams and solid dispersions for enhancement of Carvedilol dissolution rate prepared by a supercritical CO 2 process. in Polymer Testing
Elsevier Ltd., 76, 54-64.
https://doi.org/10.1016/j.polymertesting.2019.03.001

Milovanović S, Đuriš J, Dapcević A, Medarević Đ, Ibrić S, Zizović I. Soluplus ® , Eudragit ® , HPMC-AS foams and solid dispersions for enhancement of Carvedilol dissolution rate prepared by a supercritical CO 2 process. in Polymer Testing. 2019;76:54-64.
doi:10.1016/j.polymertesting.2019.03.001 .

Milovanović, S, Đuriš, Jelena, Dapcević, Aleksandra, Medarević, Đorđe, Ibrić, Svetlana, Zizović, I, "Soluplus ® , Eudragit ® , HPMC-AS foams and solid dispersions for enhancement of Carvedilol dissolution rate prepared by a supercritical CO 2 process" in Polymer Testing, 76 (2019):54-64,
https://doi.org/10.1016/j.polymertesting.2019.03.001 . .

TY  - JOUR
AU  - Žugić, Ana R.
AU  - Lunter, Dominique
AU  - Daniels, Rolf
AU  - Pantelić, Ivana
AU  - Tasić-Kostov, Marija
AU  - Tadić, Vanja M.
AU  - Mišić, Dušan
AU  - Arsić, Ivana A.
AU  - Savić, Snežana
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2588
AB  - Topical treatment of skin infections is often limited by drawbacks related to both antimicrobial agents and their vehicles. In addition, considering the growing promotion of natural therapeutic products, our objective was to develop and evaluate naturally-based emulsion system, as prospective topical formulation for skin infections-treatment. Therefore, alkyl polyglucoside surfactants were used for stabilization of a vehicle serving as potential carrier for supercritical CO2-extract of Usnea barbata, lichen with well-documented antimicrobial activity, incorporated using two protocols and three concentrations. Comprehensive physicochemical characterization suggested possible involvement of extract's particles in stabilization of the investigated system. Raman spectral imaging served as the key method in disclosing extract's particles potential to participate in the microstructure of the tested emulsion system via three mechanisms: (1) particle-particle aggregation, (2) adsorption at the oil-water interface and (3) hydrophobic particle-surfactant interactions. Stated extract-vehicle interaction proved to be correlated to the preparation procedure and extract concentration on one hand and to affect the physicochemical and biopharmaceutical features of investigated system, on the other hand. Thereafter, formulation with the best preliminary stability and liberation profile was selected for further efficiency and in vivo skin irritation potential evaluation, implying pertinent in vitro antimicrobial activity against G+ bacteria and overall satisfying preliminary safety profile.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Pharmaceutical Development and Technology
T1  - Usnea barbata CO2-supercritical extract in alkyl polyglucoside-based emulsion system: contribution of Confocal Raman imaging to the formulation development of a natural product
VL  - 21
IS  - 5
SP  - 563
EP  - 575
DO  - 10.3109/10837450.2015.1026606
ER  - 

@article{
author = "Žugić, Ana R. and Lunter, Dominique and Daniels, Rolf and Pantelić, Ivana and Tasić-Kostov, Marija and Tadić, Vanja M. and Mišić, Dušan and Arsić, Ivana A. and Savić, Snežana",
year = "2016",
abstract = "Topical treatment of skin infections is often limited by drawbacks related to both antimicrobial agents and their vehicles. In addition, considering the growing promotion of natural therapeutic products, our objective was to develop and evaluate naturally-based emulsion system, as prospective topical formulation for skin infections-treatment. Therefore, alkyl polyglucoside surfactants were used for stabilization of a vehicle serving as potential carrier for supercritical CO2-extract of Usnea barbata, lichen with well-documented antimicrobial activity, incorporated using two protocols and three concentrations. Comprehensive physicochemical characterization suggested possible involvement of extract's particles in stabilization of the investigated system. Raman spectral imaging served as the key method in disclosing extract's particles potential to participate in the microstructure of the tested emulsion system via three mechanisms: (1) particle-particle aggregation, (2) adsorption at the oil-water interface and (3) hydrophobic particle-surfactant interactions. Stated extract-vehicle interaction proved to be correlated to the preparation procedure and extract concentration on one hand and to affect the physicochemical and biopharmaceutical features of investigated system, on the other hand. Thereafter, formulation with the best preliminary stability and liberation profile was selected for further efficiency and in vivo skin irritation potential evaluation, implying pertinent in vitro antimicrobial activity against G+ bacteria and overall satisfying preliminary safety profile.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Pharmaceutical Development and Technology",
title = "Usnea barbata CO2-supercritical extract in alkyl polyglucoside-based emulsion system: contribution of Confocal Raman imaging to the formulation development of a natural product",
volume = "21",
number = "5",
pages = "563-575",
doi = "10.3109/10837450.2015.1026606"
}

Žugić, A. R., Lunter, D., Daniels, R., Pantelić, I., Tasić-Kostov, M., Tadić, V. M., Mišić, D., Arsić, I. A.,& Savić, S.. (2016). Usnea barbata CO2-supercritical extract in alkyl polyglucoside-based emulsion system: contribution of Confocal Raman imaging to the formulation development of a natural product. in Pharmaceutical Development and Technology
Taylor & Francis Ltd, Abingdon., 21(5), 563-575.
https://doi.org/10.3109/10837450.2015.1026606

Žugić AR, Lunter D, Daniels R, Pantelić I, Tasić-Kostov M, Tadić VM, Mišić D, Arsić IA, Savić S. Usnea barbata CO2-supercritical extract in alkyl polyglucoside-based emulsion system: contribution of Confocal Raman imaging to the formulation development of a natural product. in Pharmaceutical Development and Technology. 2016;21(5):563-575.
doi:10.3109/10837450.2015.1026606 .

Žugić, Ana R., Lunter, Dominique, Daniels, Rolf, Pantelić, Ivana, Tasić-Kostov, Marija, Tadić, Vanja M., Mišić, Dušan, Arsić, Ivana A., Savić, Snežana, "Usnea barbata CO2-supercritical extract in alkyl polyglucoside-based emulsion system: contribution of Confocal Raman imaging to the formulation development of a natural product" in Pharmaceutical Development and Technology, 21, no. 5 (2016):563-575,
https://doi.org/10.3109/10837450.2015.1026606 . .

TY  - JOUR
AU  - Žugić, Ana R.
AU  - Jeremić, Ivica
AU  - Isaković, Aleksandra
AU  - Arsić, Ivana A.
AU  - Savić, Snežana
AU  - Tadić, Vanja M.
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2577
AB  - There is a worldwide ongoing investigation for novel natural constituents with cytotoxic and antioxidant properties. The aim of this study was to investigate chemical profile and stated biological activities of the supercritical CO2 extract (SCE) of old man's beard compared to the extracts obtained using the conventional techniques (Soxhlet extracts and macerate). The most abundant compound identified was usnic acid, which content was inversely proportional to the polarity of the solvent used and was the highest in the SCE, which was the sample revealing the highest cytotoxic activity in tested tumor cell lines (B16 mouse melanoma and C6 rat glioma), with lower IC50 values compared to pure usnic acid. Further investigations suggested both SCE and usnic acid to induce apoptosis and/ or autophagy in B16 and C6, indicating higher cytotoxicity of SCE to be related to the higher degree of ROS production. A good correlation of usnic acid content in the extracts and their antioxidant capacity was established, extricating SCE as the most active one. Presented results support further investigations of SCE of old man's beard as a prospective therapeutic agent with potential relevance in the treatment of cancer and/ or in oxidative stress-mediated conditions.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - Evaluation of Anticancer and Antioxidant Activity of a Commercially Available CO2 Supercritical Extract of Old Man's Beard (Usnea barbata)
VL  - 11
IS  - 1
DO  - 10.1371/journal.pone.0146342
ER  - 

@article{
author = "Žugić, Ana R. and Jeremić, Ivica and Isaković, Aleksandra and Arsić, Ivana A. and Savić, Snežana and Tadić, Vanja M.",
year = "2016",
abstract = "There is a worldwide ongoing investigation for novel natural constituents with cytotoxic and antioxidant properties. The aim of this study was to investigate chemical profile and stated biological activities of the supercritical CO2 extract (SCE) of old man's beard compared to the extracts obtained using the conventional techniques (Soxhlet extracts and macerate). The most abundant compound identified was usnic acid, which content was inversely proportional to the polarity of the solvent used and was the highest in the SCE, which was the sample revealing the highest cytotoxic activity in tested tumor cell lines (B16 mouse melanoma and C6 rat glioma), with lower IC50 values compared to pure usnic acid. Further investigations suggested both SCE and usnic acid to induce apoptosis and/ or autophagy in B16 and C6, indicating higher cytotoxicity of SCE to be related to the higher degree of ROS production. A good correlation of usnic acid content in the extracts and their antioxidant capacity was established, extricating SCE as the most active one. Presented results support further investigations of SCE of old man's beard as a prospective therapeutic agent with potential relevance in the treatment of cancer and/ or in oxidative stress-mediated conditions.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "Evaluation of Anticancer and Antioxidant Activity of a Commercially Available CO2 Supercritical Extract of Old Man's Beard (Usnea barbata)",
volume = "11",
number = "1",
doi = "10.1371/journal.pone.0146342"
}

Žugić, A. R., Jeremić, I., Isaković, A., Arsić, I. A., Savić, S.,& Tadić, V. M.. (2016). Evaluation of Anticancer and Antioxidant Activity of a Commercially Available CO2 Supercritical Extract of Old Man's Beard (Usnea barbata). in PLoS One
Public Library Science, San Francisco., 11(1).
https://doi.org/10.1371/journal.pone.0146342

Žugić AR, Jeremić I, Isaković A, Arsić IA, Savić S, Tadić VM. Evaluation of Anticancer and Antioxidant Activity of a Commercially Available CO2 Supercritical Extract of Old Man's Beard (Usnea barbata). in PLoS One. 2016;11(1).
doi:10.1371/journal.pone.0146342 .

Žugić, Ana R., Jeremić, Ivica, Isaković, Aleksandra, Arsić, Ivana A., Savić, Snežana, Tadić, Vanja M., "Evaluation of Anticancer and Antioxidant Activity of a Commercially Available CO2 Supercritical Extract of Old Man's Beard (Usnea barbata)" in PLoS One, 11, no. 1 (2016),
https://doi.org/10.1371/journal.pone.0146342 . .

TY  - JOUR
AU  - Žugić, Ana R.
AU  - Lukić, Milica
AU  - Tasić-Kostov, Marija
AU  - Tadić, Vanja M.
AU  - Arsić, Ivana A.
AU  - Mišić, Dušan
AU  - Petrović, Slobodan D.
AU  - Savić, Snežana
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2477
AB  - Antimicrobial activity of Usnea barbata especially against bacteria involved in pathogenesis of various skin conditions has been well documented in literature. Nevertheless, there are no papers dealing with formulation of its isolates into topical preparations for treatment of skin infections. In present study, alkyl polyglucoside (APG)-based vehicle was developed as carrier of U. barbata CO2-supercritical extract (U-SE) that demonstrated the best anti- microbial potential in preliminary screening. For comparison, chosen extract in the same concentration and using the same procedure was incorporated into a pharmacopoeial vehicle. Comparative evaluation of physicochemical stability, efficiency and safety proved APG-based vehicle to possess certain preferential features as carrier of U-SE compared to the reference one, composing a topical formulation with potential clinical relevance in treatment of skin infections.
AB  - Antimikrobna aktivnost vrste Usnea barbata naročito protiv bakterija koje učestvuju u patogenezi različitih bolesti kože, dobro je dokumentovana u naučnoj literaturi. Uprkos tome, ne postoje radovi koji se bave formulacijom topikalnih preparata na bazi ovog lišaja namenjenih lečenju kožnih infekcija. U ovoj studiji, razvijena je podloga stablizovana alkil poliglukozidnim (APG) emulgatorom, kao potencijalni nosač za ekstrakt vrste U. barbata koji je pokazao najbolji antimikrobni potencijal u preliminarnom istraživanju nekoliko ekstrakata dobijenih upotrebom različitih ekstragenasa/postupaka. Radi poređenja, odabrani CO2-natkritični ekstrakt je inkorporiran u istoj koncentraciji (koja odgovara 2 mas.% usninske kiseline) i istim postupkom izrade u često korišćenu podlogu farmakopejskog kvaliteta, a zatim je sprovedeno uporedno istraživanje fizičko-hemijske stabilnosti, efikasnosti i bezbednosti na obe grupe uzoraka. Rezulati našeg istraživanja pokazali su da se razvijena podloga stabilizovana APG emulgatorom može smatrati pogodnim nosačem za CO2-natkritični ekstrakt vrste U. barbata, čineći topikalnu formulaciju sa potencijalnim kliničkim značajem u terapiji kožnih infekcija. Reološka istraživanja pokazala su zadovoljavajuću sposobnost emulzije stabilizovane APG emulgatorom da ostane fizički stabilna nakon dodatka CO2-natkritičnog ekstrakta vrste U. barbata, u poređenju sa farmakopejskom. Međutim, konduktometrijska merenja su pokazala određena neslaganja sa reološkim nala- zima, namećući potrebu za dodatnim istraživanjima radi konačne procene fizičke stabilnosti testiranih emulzionih sistema. Izmerene vrednosti pH tokom perioda od 90 dana čuvanja uzoraka na sobnoj temperaturi ukazale su na zadovoljavajuću preliminarnu hemijsku stabilnost svih uzoraka. Dalja istraživanja efikasnosti i bezbednosti pokazala su bolji antimikrobni potencijal uzoraka sa podlogom stabilizovanom APG emulgatorom u odnosu na tradicionalno upotrebljavanu farmakopejsku bazu poznatog kvaliteta. Zadovoljavajući preliminarni bezbedonosni profili su pokazani kako za uzorke stabilizovane APG emulgatorom tako i za referentne krem uzorke. Na osnovu navedenog, može se zaključiti da je istraživana podloga bazirana na APG emulgatoru pokazala određene povoljnije karakteristike kao nosač za CO2-natkritični ekstrakt vrste U. barbata u poređenju sa konvencionalno korišćenom podlogom, naročito uzimajući u obzir njene poboljšane senzorne karakteristike, kao osobinu koja je često odlučujuća za komplijansu pacijenata.
PB  - Savez hemijskih inženjera, Beograd
T2  - Hemijska industrija
T1  - Alkyl polyglucoside-stabilized emulsion as a prospective vehicle for usnea barbata CO2-supercritical extract: Assessing stability, safety and efficiency of a topical formulation
T1  - Emulzija stabilizovana alkil poliglukozidnim emulgatorom kao potencijalni nosač za CO2-natkritični ekstrakt vrste Usnea barbata - procena stabilnosti, bezbednosti i efikasnosti topikalne formulacije
VL  - 69
IS  - 6
SP  - 703
EP  - 712
DO  - 10.2298/HEMIND140701002Z
ER  - 

@article{
author = "Žugić, Ana R. and Lukić, Milica and Tasić-Kostov, Marija and Tadić, Vanja M. and Arsić, Ivana A. and Mišić, Dušan and Petrović, Slobodan D. and Savić, Snežana",
year = "2015",
abstract = "Antimicrobial activity of Usnea barbata especially against bacteria involved in pathogenesis of various skin conditions has been well documented in literature. Nevertheless, there are no papers dealing with formulation of its isolates into topical preparations for treatment of skin infections. In present study, alkyl polyglucoside (APG)-based vehicle was developed as carrier of U. barbata CO2-supercritical extract (U-SE) that demonstrated the best anti- microbial potential in preliminary screening. For comparison, chosen extract in the same concentration and using the same procedure was incorporated into a pharmacopoeial vehicle. Comparative evaluation of physicochemical stability, efficiency and safety proved APG-based vehicle to possess certain preferential features as carrier of U-SE compared to the reference one, composing a topical formulation with potential clinical relevance in treatment of skin infections., Antimikrobna aktivnost vrste Usnea barbata naročito protiv bakterija koje učestvuju u patogenezi različitih bolesti kože, dobro je dokumentovana u naučnoj literaturi. Uprkos tome, ne postoje radovi koji se bave formulacijom topikalnih preparata na bazi ovog lišaja namenjenih lečenju kožnih infekcija. U ovoj studiji, razvijena je podloga stablizovana alkil poliglukozidnim (APG) emulgatorom, kao potencijalni nosač za ekstrakt vrste U. barbata koji je pokazao najbolji antimikrobni potencijal u preliminarnom istraživanju nekoliko ekstrakata dobijenih upotrebom različitih ekstragenasa/postupaka. Radi poređenja, odabrani CO2-natkritični ekstrakt je inkorporiran u istoj koncentraciji (koja odgovara 2 mas.% usninske kiseline) i istim postupkom izrade u često korišćenu podlogu farmakopejskog kvaliteta, a zatim je sprovedeno uporedno istraživanje fizičko-hemijske stabilnosti, efikasnosti i bezbednosti na obe grupe uzoraka. Rezulati našeg istraživanja pokazali su da se razvijena podloga stabilizovana APG emulgatorom može smatrati pogodnim nosačem za CO2-natkritični ekstrakt vrste U. barbata, čineći topikalnu formulaciju sa potencijalnim kliničkim značajem u terapiji kožnih infekcija. Reološka istraživanja pokazala su zadovoljavajuću sposobnost emulzije stabilizovane APG emulgatorom da ostane fizički stabilna nakon dodatka CO2-natkritičnog ekstrakta vrste U. barbata, u poređenju sa farmakopejskom. Međutim, konduktometrijska merenja su pokazala određena neslaganja sa reološkim nala- zima, namećući potrebu za dodatnim istraživanjima radi konačne procene fizičke stabilnosti testiranih emulzionih sistema. Izmerene vrednosti pH tokom perioda od 90 dana čuvanja uzoraka na sobnoj temperaturi ukazale su na zadovoljavajuću preliminarnu hemijsku stabilnost svih uzoraka. Dalja istraživanja efikasnosti i bezbednosti pokazala su bolji antimikrobni potencijal uzoraka sa podlogom stabilizovanom APG emulgatorom u odnosu na tradicionalno upotrebljavanu farmakopejsku bazu poznatog kvaliteta. Zadovoljavajući preliminarni bezbedonosni profili su pokazani kako za uzorke stabilizovane APG emulgatorom tako i za referentne krem uzorke. Na osnovu navedenog, može se zaključiti da je istraživana podloga bazirana na APG emulgatoru pokazala određene povoljnije karakteristike kao nosač za CO2-natkritični ekstrakt vrste U. barbata u poređenju sa konvencionalno korišćenom podlogom, naročito uzimajući u obzir njene poboljšane senzorne karakteristike, kao osobinu koja je često odlučujuća za komplijansu pacijenata.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Hemijska industrija",
title = "Alkyl polyglucoside-stabilized emulsion as a prospective vehicle for usnea barbata CO2-supercritical extract: Assessing stability, safety and efficiency of a topical formulation, Emulzija stabilizovana alkil poliglukozidnim emulgatorom kao potencijalni nosač za CO2-natkritični ekstrakt vrste Usnea barbata - procena stabilnosti, bezbednosti i efikasnosti topikalne formulacije",
volume = "69",
number = "6",
pages = "703-712",
doi = "10.2298/HEMIND140701002Z"
}

Žugić, A. R., Lukić, M., Tasić-Kostov, M., Tadić, V. M., Arsić, I. A., Mišić, D., Petrović, S. D.,& Savić, S.. (2015). Alkyl polyglucoside-stabilized emulsion as a prospective vehicle for usnea barbata CO2-supercritical extract: Assessing stability, safety and efficiency of a topical formulation. in Hemijska industrija
Savez hemijskih inženjera, Beograd., 69(6), 703-712.
https://doi.org/10.2298/HEMIND140701002Z

Žugić AR, Lukić M, Tasić-Kostov M, Tadić VM, Arsić IA, Mišić D, Petrović SD, Savić S. Alkyl polyglucoside-stabilized emulsion as a prospective vehicle for usnea barbata CO2-supercritical extract: Assessing stability, safety and efficiency of a topical formulation. in Hemijska industrija. 2015;69(6):703-712.
doi:10.2298/HEMIND140701002Z .

Žugić, Ana R., Lukić, Milica, Tasić-Kostov, Marija, Tadić, Vanja M., Arsić, Ivana A., Mišić, Dušan, Petrović, Slobodan D., Savić, Snežana, "Alkyl polyglucoside-stabilized emulsion as a prospective vehicle for usnea barbata CO2-supercritical extract: Assessing stability, safety and efficiency of a topical formulation" in Hemijska industrija, 69, no. 6 (2015):703-712,
https://doi.org/10.2298/HEMIND140701002Z . .

TY  - JOUR
AU  - Arsić, Ivana A.
AU  - Tadić, Vanja M.
AU  - Đorđević, Sofija
AU  - Žugić, Ana R.
AU  - Vujić, Zorica
AU  - Petrović, Slobodan D.
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2267
AB  - Recently, research efforts have been directed toward medicinal plants and their extracts, as important sources of natural antioxidant. Lots of biologically active compounds are responsible for the antioxidant effects of yarrow - Achillea millefilium L. extracts. The aim of our study was to determine which of the process parameters of pressure enhanced solvent extraction of Millefolii herba is significant for its efficiency and weather there are interactions between the examined parameters. Compression time, decompression time and the number of cycles were identified as independent variables, while the content of total flavonoids, tannins and total polyphenols were selected as dependent variables. For obtaining the extract of M. herba, rich in antioxidative ingredients, compression time should be set on its higher level (2.0 min), decompression time on its lower level (1.30 min) and the number of cycles on its higher level (99).
AB  - Oksidativna degradacija sastojaka hrane dovodi do promene njenog mirisa i ukusa, čime se narušava njen nutritivni i senzorni kvalitet. Iz navedenog razloga je neophodno dodavati antioksidante tokom tehnološkog procesa izrade namirnica. Lekovito bilje i njihovi izolati predmet su istraživanja, pored ostalog i kao izvor antioksidantnih materija i alternativa sintetičkim antioksidansima za primenu u industriji hrane. Istraživanja velikog broja biljaka ukazala su da skoro sve poseduju određena antioksidativna svojstava. Hajdučka trava - Achillea millefilium L. (Asteraceae) i njeni ekstrakti, zahvaljujući prisustvu, pre svega, polifenola, flavonoida i tanina, ispoljavaju antioksidativni efekat. Sadržaj ovih materija u izolatima zavisi od njihove koncentracije u polaznom biljnom materijalu ali i od načina/uslova ekstrakcije. Cilj našeg ispitivanja bio je da utvrdimo optimalne vrednosti procesnih parametara za izvođenje pritiskom ubrzane ekstrakcije iz herbe hajdučke trave (Millefolii herba), radi dobijanja ekstrakata bogatih antioksidativnim materijama, kao i da utvrdimo zavisnost koja postoji između procesnih parametara. Ekstrakcija je obavljena u Timatic mikro ekstraktoru. Takozvano vreme kompresije, vreme dekompresije i broj ciklusa kruženja ekstrakcionog sredstva predstavljaju nezavisno promenljive veličine, dok su sadržaj ukupnih flavonoida, tanina, ukupnih polifenola kao i indeks refrakcije, suvi ostatak, relativna gustina, sadržaj etanola i pH vrednost zavisno promenljive veličine. Vreme kompresije bilo je 1 odnosno 2 minuta, vrednost vremena dekompresije 1,3 i 2 min, dok su ekstrakcije obavljene uz minimalno 20 odnosno maksimalno 99 ciklusa. Kao ekstragens korišćen je 70% (V/V) etanol. Droga: ekstrakt odnos iznosio je 1:3. Radni pritisak kretao se u opsegu od 1 do 99 bar. Primenjen je 23 faktorski dizajn, odnosno urađeno je osam ekstrakcija herbe hajdučke trave. Statistička analiza eksperimentalnih rezultata izvršena je upotrebom softvera Design Expert 7.0. U cilju potpune optimizacije primenjena je multivarijantna metodologija upotrebom Derringer funkcije poželjnih odgovora. Proces optimizacije postupka ekstrakcije pokazao je da je za izradu ekstrakata herbe hajdučke trave bogatih sadržajem antioksidativnih materija (flavonoidi, tanini i polifenoli), potrebno podesiti vreme kompresije na najviši nivo (2,0 min), vreme dekompresije na najniži (1,30 min) i vršiti ekstrakciju uz maksimalni broj ciklusa (99).
PB  - Savez hemijskih inženjera, Beograd
T2  - Hemijska industrija
T1  - Optimization of extraction of antioxidant components from yarrow herb
T1  - Optimizacija ekstrakcije antioksidantnih komponenata iz herbe hajdučke trave
VL  - 68
IS  - 4
SP  - 511
EP  - 517
DO  - 10.2298/HEMIND130910076A
ER  - 

@article{
author = "Arsić, Ivana A. and Tadić, Vanja M. and Đorđević, Sofija and Žugić, Ana R. and Vujić, Zorica and Petrović, Slobodan D.",
year = "2014",
abstract = "Recently, research efforts have been directed toward medicinal plants and their extracts, as important sources of natural antioxidant. Lots of biologically active compounds are responsible for the antioxidant effects of yarrow - Achillea millefilium L. extracts. The aim of our study was to determine which of the process parameters of pressure enhanced solvent extraction of Millefolii herba is significant for its efficiency and weather there are interactions between the examined parameters. Compression time, decompression time and the number of cycles were identified as independent variables, while the content of total flavonoids, tannins and total polyphenols were selected as dependent variables. For obtaining the extract of M. herba, rich in antioxidative ingredients, compression time should be set on its higher level (2.0 min), decompression time on its lower level (1.30 min) and the number of cycles on its higher level (99)., Oksidativna degradacija sastojaka hrane dovodi do promene njenog mirisa i ukusa, čime se narušava njen nutritivni i senzorni kvalitet. Iz navedenog razloga je neophodno dodavati antioksidante tokom tehnološkog procesa izrade namirnica. Lekovito bilje i njihovi izolati predmet su istraživanja, pored ostalog i kao izvor antioksidantnih materija i alternativa sintetičkim antioksidansima za primenu u industriji hrane. Istraživanja velikog broja biljaka ukazala su da skoro sve poseduju određena antioksidativna svojstava. Hajdučka trava - Achillea millefilium L. (Asteraceae) i njeni ekstrakti, zahvaljujući prisustvu, pre svega, polifenola, flavonoida i tanina, ispoljavaju antioksidativni efekat. Sadržaj ovih materija u izolatima zavisi od njihove koncentracije u polaznom biljnom materijalu ali i od načina/uslova ekstrakcije. Cilj našeg ispitivanja bio je da utvrdimo optimalne vrednosti procesnih parametara za izvođenje pritiskom ubrzane ekstrakcije iz herbe hajdučke trave (Millefolii herba), radi dobijanja ekstrakata bogatih antioksidativnim materijama, kao i da utvrdimo zavisnost koja postoji između procesnih parametara. Ekstrakcija je obavljena u Timatic mikro ekstraktoru. Takozvano vreme kompresije, vreme dekompresije i broj ciklusa kruženja ekstrakcionog sredstva predstavljaju nezavisno promenljive veličine, dok su sadržaj ukupnih flavonoida, tanina, ukupnih polifenola kao i indeks refrakcije, suvi ostatak, relativna gustina, sadržaj etanola i pH vrednost zavisno promenljive veličine. Vreme kompresije bilo je 1 odnosno 2 minuta, vrednost vremena dekompresije 1,3 i 2 min, dok su ekstrakcije obavljene uz minimalno 20 odnosno maksimalno 99 ciklusa. Kao ekstragens korišćen je 70% (V/V) etanol. Droga: ekstrakt odnos iznosio je 1:3. Radni pritisak kretao se u opsegu od 1 do 99 bar. Primenjen je 23 faktorski dizajn, odnosno urađeno je osam ekstrakcija herbe hajdučke trave. Statistička analiza eksperimentalnih rezultata izvršena je upotrebom softvera Design Expert 7.0. U cilju potpune optimizacije primenjena je multivarijantna metodologija upotrebom Derringer funkcije poželjnih odgovora. Proces optimizacije postupka ekstrakcije pokazao je da je za izradu ekstrakata herbe hajdučke trave bogatih sadržajem antioksidativnih materija (flavonoidi, tanini i polifenoli), potrebno podesiti vreme kompresije na najviši nivo (2,0 min), vreme dekompresije na najniži (1,30 min) i vršiti ekstrakciju uz maksimalni broj ciklusa (99).",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Hemijska industrija",
title = "Optimization of extraction of antioxidant components from yarrow herb, Optimizacija ekstrakcije antioksidantnih komponenata iz herbe hajdučke trave",
volume = "68",
number = "4",
pages = "511-517",
doi = "10.2298/HEMIND130910076A"
}

Arsić, I. A., Tadić, V. M., Đorđević, S., Žugić, A. R., Vujić, Z.,& Petrović, S. D.. (2014). Optimization of extraction of antioxidant components from yarrow herb. in Hemijska industrija
Savez hemijskih inženjera, Beograd., 68(4), 511-517.
https://doi.org/10.2298/HEMIND130910076A

Arsić IA, Tadić VM, Đorđević S, Žugić AR, Vujić Z, Petrović SD. Optimization of extraction of antioxidant components from yarrow herb. in Hemijska industrija. 2014;68(4):511-517.
doi:10.2298/HEMIND130910076A .

Arsić, Ivana A., Tadić, Vanja M., Đorđević, Sofija, Žugić, Ana R., Vujić, Zorica, Petrović, Slobodan D., "Optimization of extraction of antioxidant components from yarrow herb" in Hemijska industrija, 68, no. 4 (2014):511-517,
https://doi.org/10.2298/HEMIND130910076A . .

TY  - JOUR
AU  - Đorđević, Sofija
AU  - Tadić, Vanja M.
AU  - Petrović, Silvana
AU  - Kukić-Marković, Jelena
AU  - Dobrić, Silva
AU  - Milenković, Marina
AU  - Hadžifejzović, Nihad
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1688
AB  - The present study describes investigation on chemical profile and pharmacological properties of hydromethanolic extracts of Carlina acaulis and C. acanthifolia roots and herbs. In order to investigate the antioxidant properties several methods were applied: FRAP (Ferric Reducing Antioxidant Power) assay, TBA assay (Fe2+/ascorbic induced lipid peroxidation) and scavenging of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and OH radicals. In assessing anti-inflammatory activity the carrageenan-induced rat paw oedema test was used, with indomethacin as a reference drug. Anti-ulcer activity of the extracts was investigated using ethanol-induced stress ulcer in rats and ranitidine as a reference drug. Antimicrobial activity was tested on five bacterial strains and one strain of fungi using the agar diffusion method. Investigated extracts exhibited considerable dose-dependent antioxidant, anti-inflammatory and anti-ulcer activities. Extracts of herbs were more active than the corresponding root extracts. All extracts exhibited moderate activity against all tested microorganisms. Herb extracts of C. acaulis and C. acanthifolia, as well as their root extracts exhibited similar bioactivity, which could be explained with their similar chemical composition revealed by HPLC analysis. The pharmacological activities of investigated Carlina extracts demonstrated here will serve as the basis for their further investigations in order to estimate their impact on human health and nutrition.
PB  - Inst Materials Physics, Bucharest
T2  - Digest Journal of Nanomaterials and Biostructures
T1  - Bioactivity assays on Carlina acaulis and C. acanthifolia root and herb extracts
VL  - 7
IS  - 3
SP  - 1213
EP  - 1222
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1688
ER  - 

@article{
author = "Đorđević, Sofija and Tadić, Vanja M. and Petrović, Silvana and Kukić-Marković, Jelena and Dobrić, Silva and Milenković, Marina and Hadžifejzović, Nihad",
year = "2012",
abstract = "The present study describes investigation on chemical profile and pharmacological properties of hydromethanolic extracts of Carlina acaulis and C. acanthifolia roots and herbs. In order to investigate the antioxidant properties several methods were applied: FRAP (Ferric Reducing Antioxidant Power) assay, TBA assay (Fe2+/ascorbic induced lipid peroxidation) and scavenging of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and OH radicals. In assessing anti-inflammatory activity the carrageenan-induced rat paw oedema test was used, with indomethacin as a reference drug. Anti-ulcer activity of the extracts was investigated using ethanol-induced stress ulcer in rats and ranitidine as a reference drug. Antimicrobial activity was tested on five bacterial strains and one strain of fungi using the agar diffusion method. Investigated extracts exhibited considerable dose-dependent antioxidant, anti-inflammatory and anti-ulcer activities. Extracts of herbs were more active than the corresponding root extracts. All extracts exhibited moderate activity against all tested microorganisms. Herb extracts of C. acaulis and C. acanthifolia, as well as their root extracts exhibited similar bioactivity, which could be explained with their similar chemical composition revealed by HPLC analysis. The pharmacological activities of investigated Carlina extracts demonstrated here will serve as the basis for their further investigations in order to estimate their impact on human health and nutrition.",
publisher = "Inst Materials Physics, Bucharest",
journal = "Digest Journal of Nanomaterials and Biostructures",
title = "Bioactivity assays on Carlina acaulis and C. acanthifolia root and herb extracts",
volume = "7",
number = "3",
pages = "1213-1222",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1688"
}

Đorđević, S., Tadić, V. M., Petrović, S., Kukić-Marković, J., Dobrić, S., Milenković, M.,& Hadžifejzović, N.. (2012). Bioactivity assays on Carlina acaulis and C. acanthifolia root and herb extracts. in Digest Journal of Nanomaterials and Biostructures
Inst Materials Physics, Bucharest., 7(3), 1213-1222.
https://hdl.handle.net/21.15107/rcub_farfar_1688

Đorđević S, Tadić VM, Petrović S, Kukić-Marković J, Dobrić S, Milenković M, Hadžifejzović N. Bioactivity assays on Carlina acaulis and C. acanthifolia root and herb extracts. in Digest Journal of Nanomaterials and Biostructures. 2012;7(3):1213-1222.
https://hdl.handle.net/21.15107/rcub_farfar_1688 .

Đorđević, Sofija, Tadić, Vanja M., Petrović, Silvana, Kukić-Marković, Jelena, Dobrić, Silva, Milenković, Marina, Hadžifejzović, Nihad, "Bioactivity assays on Carlina acaulis and C. acanthifolia root and herb extracts" in Digest Journal of Nanomaterials and Biostructures, 7, no. 3 (2012):1213-1222,
https://hdl.handle.net/21.15107/rcub_farfar_1688 .

TY  - JOUR
AU  - Zizović, Irena
AU  - Ivanović, Jasna
AU  - Mišić, Dušan
AU  - Stamenić, Marko
AU  - Đorđević, Sofija
AU  - Kukić-Marković, Jelena
AU  - Petrović, Slobodan D.
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1665
AB  - The number of cases of fatal infections in humans and animals caused by multiresistant and panresistant bacterial strains has been dramatically increased over the past 10 years on almost every continent. Therefore, implementation of newer technologies in order to develop processes for isolation of substances with strong antibacterial activities from natural sources is of increasing interest. In this study, advantage of high pressure processing and supercritical fluid extraction with carbon dioxide in isolation of active principles from lichen Usnea barbata L was presented. Supercritical extraction showed extremely higher selectivity toward usnic acid compared to the conventional techniques tested. In order to simulate the process and estimate mass transfer coefficients, the new mathematical model of Sovova (2011) was applied. Supercritical extract obtained at 40 C and 30 MPa showed stronger antibacterial activity against the most of tested strains compared to pure usnic acid and extracts obtained using ethanol as a solvent. Further, supercritical extract with lower usnic acid content expressed stronger antibacterial activity against the majority of tested strains including MRSA strains compared to the pure usnic acid and the supercritical extract with the higher usnic acid content. The same extract was far more effective than ampicillin against Streptococcus uberis, all tested Enterococcus faecalis, MRSA and MRSA ATCC 33591. Against few tested strains, the supercritical extract was superior to erythromycin and penicillin as well. These findings justified possible benefits of inclusion of supercritical extracts into pharmaceutical and cosmetic formulations. Applied mathematical model described the SFE process with high accuracy.
PB  - Elsevier Science BV, Amsterdam
T2  - Journal of Supercritical Fluids
T1  - SFE as a superior technique for isolation of extracts with strong antibacterial activities from lichen Usnea barbata L.
VL  - 72
SP  - 7
EP  - 14
DO  - 10.1016/j.supflu.2012.07.018
ER  - 

@article{
author = "Zizović, Irena and Ivanović, Jasna and Mišić, Dušan and Stamenić, Marko and Đorđević, Sofija and Kukić-Marković, Jelena and Petrović, Slobodan D.",
year = "2012",
abstract = "The number of cases of fatal infections in humans and animals caused by multiresistant and panresistant bacterial strains has been dramatically increased over the past 10 years on almost every continent. Therefore, implementation of newer technologies in order to develop processes for isolation of substances with strong antibacterial activities from natural sources is of increasing interest. In this study, advantage of high pressure processing and supercritical fluid extraction with carbon dioxide in isolation of active principles from lichen Usnea barbata L was presented. Supercritical extraction showed extremely higher selectivity toward usnic acid compared to the conventional techniques tested. In order to simulate the process and estimate mass transfer coefficients, the new mathematical model of Sovova (2011) was applied. Supercritical extract obtained at 40 C and 30 MPa showed stronger antibacterial activity against the most of tested strains compared to pure usnic acid and extracts obtained using ethanol as a solvent. Further, supercritical extract with lower usnic acid content expressed stronger antibacterial activity against the majority of tested strains including MRSA strains compared to the pure usnic acid and the supercritical extract with the higher usnic acid content. The same extract was far more effective than ampicillin against Streptococcus uberis, all tested Enterococcus faecalis, MRSA and MRSA ATCC 33591. Against few tested strains, the supercritical extract was superior to erythromycin and penicillin as well. These findings justified possible benefits of inclusion of supercritical extracts into pharmaceutical and cosmetic formulations. Applied mathematical model described the SFE process with high accuracy.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Journal of Supercritical Fluids",
title = "SFE as a superior technique for isolation of extracts with strong antibacterial activities from lichen Usnea barbata L.",
volume = "72",
pages = "7-14",
doi = "10.1016/j.supflu.2012.07.018"
}

Zizović, I., Ivanović, J., Mišić, D., Stamenić, M., Đorđević, S., Kukić-Marković, J.,& Petrović, S. D.. (2012). SFE as a superior technique for isolation of extracts with strong antibacterial activities from lichen Usnea barbata L.. in Journal of Supercritical Fluids
Elsevier Science BV, Amsterdam., 72, 7-14.
https://doi.org/10.1016/j.supflu.2012.07.018

Zizović I, Ivanović J, Mišić D, Stamenić M, Đorđević S, Kukić-Marković J, Petrović SD. SFE as a superior technique for isolation of extracts with strong antibacterial activities from lichen Usnea barbata L.. in Journal of Supercritical Fluids. 2012;72:7-14.
doi:10.1016/j.supflu.2012.07.018 .

Zizović, Irena, Ivanović, Jasna, Mišić, Dušan, Stamenić, Marko, Đorđević, Sofija, Kukić-Marković, Jelena, Petrović, Slobodan D., "SFE as a superior technique for isolation of extracts with strong antibacterial activities from lichen Usnea barbata L." in Journal of Supercritical Fluids, 72 (2012):7-14,
https://doi.org/10.1016/j.supflu.2012.07.018 . .

TY  - JOUR
AU  - Tasić-Kostov, Marija
AU  - Pavlović, Dragana R.
AU  - Lukić, Milica
AU  - Jaksić, I.
AU  - Arsić, I.
AU  - Savić, Snežana
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1643
AB  - Synopsis Cosmeceutical antioxidants may protect the skin against oxidative injury, involved in the pathogenesis of many skin disorders. However, an unsuitable topical delivery system with compromising safety profile can affect the efficacy of an antioxidant active. This study investigated the antioxidant potential of lactobionic acid (LA), a newer cosmeceutical active, per se (in solution) and incorporated into natural alkyl polyglucoside (APG) emulsifier-based system using 1,1-diphenyl-2-picrylhydrazyl free radical scavenging and lipid peroxidation inhibition assays. The a-tocopherol was used as a reference compound. The physical stability (using rheology, polarization microscopy, pH and conductivity measurements) of an Alkyl glucoside-based emulsion was evaluated with and without the active (LA); colloidal structure was assessed using polarization and transmission electron microscopy, rheology, thermal and texture analysis. Additionally, the safety profile and moisturizing potential were investigated using the methods of skin bioengineering. Good physical stability and applicative characteristics were obtained although LA strongly influenced the colloidal structure of the vehicle. LA per se and in APG-based emulsion showed satisfying antioxidant activity that promotes it as mild multifunctional cosmeceutical efficient in the treatment and prevention of the photoaged skin. Employed assays were shown as suitable for the antioxidant activity evaluation of LA in APG-based emulsions, but not for a-tocopherol in the same vehicle.
PB  - Wiley-Blackwell, Hoboken
T2  - International Journal of Cosmetic Science
T1  - Lactobionic acid as antioxidant and moisturizing active in alkyl polyglucoside-based topical emulsions: the colloidal structure, stability and efficacy evaluation
VL  - 34
IS  - 5
SP  - 424
EP  - 434
DO  - 10.1111/j.1468-2494.2012.00732.x
ER  - 

@article{
author = "Tasić-Kostov, Marija and Pavlović, Dragana R. and Lukić, Milica and Jaksić, I. and Arsić, I. and Savić, Snežana",
year = "2012",
abstract = "Synopsis Cosmeceutical antioxidants may protect the skin against oxidative injury, involved in the pathogenesis of many skin disorders. However, an unsuitable topical delivery system with compromising safety profile can affect the efficacy of an antioxidant active. This study investigated the antioxidant potential of lactobionic acid (LA), a newer cosmeceutical active, per se (in solution) and incorporated into natural alkyl polyglucoside (APG) emulsifier-based system using 1,1-diphenyl-2-picrylhydrazyl free radical scavenging and lipid peroxidation inhibition assays. The a-tocopherol was used as a reference compound. The physical stability (using rheology, polarization microscopy, pH and conductivity measurements) of an Alkyl glucoside-based emulsion was evaluated with and without the active (LA); colloidal structure was assessed using polarization and transmission electron microscopy, rheology, thermal and texture analysis. Additionally, the safety profile and moisturizing potential were investigated using the methods of skin bioengineering. Good physical stability and applicative characteristics were obtained although LA strongly influenced the colloidal structure of the vehicle. LA per se and in APG-based emulsion showed satisfying antioxidant activity that promotes it as mild multifunctional cosmeceutical efficient in the treatment and prevention of the photoaged skin. Employed assays were shown as suitable for the antioxidant activity evaluation of LA in APG-based emulsions, but not for a-tocopherol in the same vehicle.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "International Journal of Cosmetic Science",
title = "Lactobionic acid as antioxidant and moisturizing active in alkyl polyglucoside-based topical emulsions: the colloidal structure, stability and efficacy evaluation",
volume = "34",
number = "5",
pages = "424-434",
doi = "10.1111/j.1468-2494.2012.00732.x"
}

Tasić-Kostov, M., Pavlović, D. R., Lukić, M., Jaksić, I., Arsić, I.,& Savić, S.. (2012). Lactobionic acid as antioxidant and moisturizing active in alkyl polyglucoside-based topical emulsions: the colloidal structure, stability and efficacy evaluation. in International Journal of Cosmetic Science
Wiley-Blackwell, Hoboken., 34(5), 424-434.
https://doi.org/10.1111/j.1468-2494.2012.00732.x

Tasić-Kostov M, Pavlović DR, Lukić M, Jaksić I, Arsić I, Savić S. Lactobionic acid as antioxidant and moisturizing active in alkyl polyglucoside-based topical emulsions: the colloidal structure, stability and efficacy evaluation. in International Journal of Cosmetic Science. 2012;34(5):424-434.
doi:10.1111/j.1468-2494.2012.00732.x .

Tasić-Kostov, Marija, Pavlović, Dragana R., Lukić, Milica, Jaksić, I., Arsić, I., Savić, Snežana, "Lactobionic acid as antioxidant and moisturizing active in alkyl polyglucoside-based topical emulsions: the colloidal structure, stability and efficacy evaluation" in International Journal of Cosmetic Science, 34, no. 5 (2012):424-434,
https://doi.org/10.1111/j.1468-2494.2012.00732.x . .

TY  - JOUR
AU  - Tasić-Kostov, Marija
AU  - Reichl, Stephan
AU  - Lukić, Milica
AU  - Jaksić, I. N.
AU  - Savić, Snežana
PY  - 2011
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1513
AB  - Moisturizing creams are the most prescribed products in dermatology, essential in maintaining healthy skin as well as in the topical treatment of some diseases. The irritation potential of commonly used emulsifiers and moisturizing ingredients, but also their mutual interactions, could affect the functionality and safety of those dermopharmaceutics. The aim of this study was to promote moisturizing alkyl polyglucoside (APG)-based emulsion as vehicle for lactobionic acid (LA), advantageous representative of the alphahydroxyacids (AHAs)-multifunctional moisturizers, assessing the safety for use (in vitro acute skin irritation test using cytotoxicity assay compared with in vivo data obtained using skin bioengineering methods) and in vivo moisturizing capacity (bioengineering of the skin). In order to investigate possible interactions between APG mild natural emulsifier-based emulsion and LA, a deeper insight into the colloidal structure of the placebo and the emulsion with LA was given using polarization and transmission electron microscopy, rheology, thermal and texture analysis. This study showed that APG-based emulsions could be promoted as safe cosmetic/dermopharmaceutical vehicles and carriers for extremely acidic and hygroscopic AHA class of actives (specifically LA); prospective safety for human use of both APG and LA with the correlation between in vivo and in vitro findings was shown. However, it was revealed that LA strongly influenced the colloidal structure of the emulsion based on APGs and promoted the formation of lamellar structures which reflects onto the mode of water distribution within the cream. The advantageous skin hydrating potential of LA-containing emulsion vs. placebo was unlikely to be achieved, pointing that emulsions stabilized by lamellar liquid crystalline structures probably are not satisfying carriers for highly hygroscopic actives in order to reach the full moisturizing potential. Safe and effective use on dry skin is presumed.
PB  - Govi-Verlag  Pharmazeutischer Verlag Gmbh, Eschborn
T2  - Pharmazie
T1  - Does lactobionic acid affect the colloidal structure and skin moisturizing potential of the alkyl polyglucoside-based emulsion systems?
VL  - 66
IS  - 11
SP  - 862
EP  - 870
DO  - 10.1691/ph.2011.1066
ER  - 

@article{
author = "Tasić-Kostov, Marija and Reichl, Stephan and Lukić, Milica and Jaksić, I. N. and Savić, Snežana",
year = "2011",
abstract = "Moisturizing creams are the most prescribed products in dermatology, essential in maintaining healthy skin as well as in the topical treatment of some diseases. The irritation potential of commonly used emulsifiers and moisturizing ingredients, but also their mutual interactions, could affect the functionality and safety of those dermopharmaceutics. The aim of this study was to promote moisturizing alkyl polyglucoside (APG)-based emulsion as vehicle for lactobionic acid (LA), advantageous representative of the alphahydroxyacids (AHAs)-multifunctional moisturizers, assessing the safety for use (in vitro acute skin irritation test using cytotoxicity assay compared with in vivo data obtained using skin bioengineering methods) and in vivo moisturizing capacity (bioengineering of the skin). In order to investigate possible interactions between APG mild natural emulsifier-based emulsion and LA, a deeper insight into the colloidal structure of the placebo and the emulsion with LA was given using polarization and transmission electron microscopy, rheology, thermal and texture analysis. This study showed that APG-based emulsions could be promoted as safe cosmetic/dermopharmaceutical vehicles and carriers for extremely acidic and hygroscopic AHA class of actives (specifically LA); prospective safety for human use of both APG and LA with the correlation between in vivo and in vitro findings was shown. However, it was revealed that LA strongly influenced the colloidal structure of the emulsion based on APGs and promoted the formation of lamellar structures which reflects onto the mode of water distribution within the cream. The advantageous skin hydrating potential of LA-containing emulsion vs. placebo was unlikely to be achieved, pointing that emulsions stabilized by lamellar liquid crystalline structures probably are not satisfying carriers for highly hygroscopic actives in order to reach the full moisturizing potential. Safe and effective use on dry skin is presumed.",
publisher = "Govi-Verlag  Pharmazeutischer Verlag Gmbh, Eschborn",
journal = "Pharmazie",
title = "Does lactobionic acid affect the colloidal structure and skin moisturizing potential of the alkyl polyglucoside-based emulsion systems?",
volume = "66",
number = "11",
pages = "862-870",
doi = "10.1691/ph.2011.1066"
}

Tasić-Kostov, M., Reichl, S., Lukić, M., Jaksić, I. N.,& Savić, S.. (2011). Does lactobionic acid affect the colloidal structure and skin moisturizing potential of the alkyl polyglucoside-based emulsion systems?. in Pharmazie
Govi-Verlag  Pharmazeutischer Verlag Gmbh, Eschborn., 66(11), 862-870.
https://doi.org/10.1691/ph.2011.1066

Tasić-Kostov M, Reichl S, Lukić M, Jaksić IN, Savić S. Does lactobionic acid affect the colloidal structure and skin moisturizing potential of the alkyl polyglucoside-based emulsion systems?. in Pharmazie. 2011;66(11):862-870.
doi:10.1691/ph.2011.1066 .

Tasić-Kostov, Marija, Reichl, Stephan, Lukić, Milica, Jaksić, I. N., Savić, Snežana, "Does lactobionic acid affect the colloidal structure and skin moisturizing potential of the alkyl polyglucoside-based emulsion systems?" in Pharmazie, 66, no. 11 (2011):862-870,
https://doi.org/10.1691/ph.2011.1066 . .

TY  - JOUR
AU  - Tasić-Kostov, Marija
AU  - Reichl, Stephan
AU  - Lukić, Milica
AU  - Jaksić, I.
AU  - Savić, Snežana
PY  - 2011
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1490
AB  - There is a growing need for research into new skin and environmentally-friendly actives and surfactants in the field of cosmetics. This paper focuses on different natural alkylpolyglucoside (APG) emulsifiers (cetearyl alcohol and coco glucoside; arachidyl behenyl alcohol and arachidyl glucoside), which can form lyotropic liquid crystals, in order to promote them as cosmetic excipients. APG-based emulsions as carriers for extremely hygroscopic and acidic cosmetic active, lactobionic acid (LA), belonging to alpha-hydroxy acids (AHAs) were also studied. In the first part of the study, physicochemical properties (assessed by rheology, polarization microscopy and pH and conductivity measurements) and in vitro safety profile (skin irritation test using a cytotoxicity assay) of vehicles were determined in order to compare and estimate their applicability as cosmetic excipients. In the second part, the same methods were conducted in order to establish how those emulsions perform as carriers for different percents of LA. The study showed that both vehicles based on different APGs could be promoted as cosmetic excipients. Either of the APG based emulsions could be vehicles for 6% LA. Cetearyl alcohol and coco glucoside, more hydrophillic emulsifier, is acceptable carrier for 10% LA due to better cytotoxicity profile. The importance of a better cytotoxicity profile without neglecting satisfying physical stability for cosmetic formulations was emphasized.
PB  - Servizi Editoriali Assoc Srl, Como
T2  - RSC Advances
T1  - Two alkyl polyglucoside natural surfactants varying in chain length in stabilization of lactobionic acid containing emulsions: physicochemical characterization and in vitro irritation potential assessment
VL  - 88
IS  - 4
SP  - 256
EP  - 264
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1490
ER  - 

@article{
author = "Tasić-Kostov, Marija and Reichl, Stephan and Lukić, Milica and Jaksić, I. and Savić, Snežana",
year = "2011",
abstract = "There is a growing need for research into new skin and environmentally-friendly actives and surfactants in the field of cosmetics. This paper focuses on different natural alkylpolyglucoside (APG) emulsifiers (cetearyl alcohol and coco glucoside; arachidyl behenyl alcohol and arachidyl glucoside), which can form lyotropic liquid crystals, in order to promote them as cosmetic excipients. APG-based emulsions as carriers for extremely hygroscopic and acidic cosmetic active, lactobionic acid (LA), belonging to alpha-hydroxy acids (AHAs) were also studied. In the first part of the study, physicochemical properties (assessed by rheology, polarization microscopy and pH and conductivity measurements) and in vitro safety profile (skin irritation test using a cytotoxicity assay) of vehicles were determined in order to compare and estimate their applicability as cosmetic excipients. In the second part, the same methods were conducted in order to establish how those emulsions perform as carriers for different percents of LA. The study showed that both vehicles based on different APGs could be promoted as cosmetic excipients. Either of the APG based emulsions could be vehicles for 6% LA. Cetearyl alcohol and coco glucoside, more hydrophillic emulsifier, is acceptable carrier for 10% LA due to better cytotoxicity profile. The importance of a better cytotoxicity profile without neglecting satisfying physical stability for cosmetic formulations was emphasized.",
publisher = "Servizi Editoriali Assoc Srl, Como",
journal = "RSC Advances",
title = "Two alkyl polyglucoside natural surfactants varying in chain length in stabilization of lactobionic acid containing emulsions: physicochemical characterization and in vitro irritation potential assessment",
volume = "88",
number = "4",
pages = "256-264",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1490"
}

Tasić-Kostov, M., Reichl, S., Lukić, M., Jaksić, I.,& Savić, S.. (2011). Two alkyl polyglucoside natural surfactants varying in chain length in stabilization of lactobionic acid containing emulsions: physicochemical characterization and in vitro irritation potential assessment. in RSC Advances
Servizi Editoriali Assoc Srl, Como., 88(4), 256-264.
https://hdl.handle.net/21.15107/rcub_farfar_1490

Tasić-Kostov M, Reichl S, Lukić M, Jaksić I, Savić S. Two alkyl polyglucoside natural surfactants varying in chain length in stabilization of lactobionic acid containing emulsions: physicochemical characterization and in vitro irritation potential assessment. in RSC Advances. 2011;88(4):256-264.
https://hdl.handle.net/21.15107/rcub_farfar_1490 .

Tasić-Kostov, Marija, Reichl, Stephan, Lukić, Milica, Jaksić, I., Savić, Snežana, "Two alkyl polyglucoside natural surfactants varying in chain length in stabilization of lactobionic acid containing emulsions: physicochemical characterization and in vitro irritation potential assessment" in RSC Advances, 88, no. 4 (2011):256-264,
https://hdl.handle.net/21.15107/rcub_farfar_1490 .