TY  - JOUR
AU  - Aminjafari, Akram
AU  - Miroliaei, Mehran
AU  - Angelova, Violina T.
AU  - Emamzadeh, Rahman
AU  - Đukić, Mirjana
AU  - Đurić, Ana
AU  - Saso, Luciano
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2679
AB  - Background: Synthesized aminocoumarins are heterocyclic compounds possessing potential for the treatment of insulin-dependent diabetes mellitus with unexplored anti-glycative action. Results: In this study 4-aminocoumarin derivatives (4-ACDs) were evaluated in vitro for antiglycation (AG) activities by using the human serum albumin (HSA)/glucose system, for 8 weeks of incubation. The glycation and conformational alteration of HSA in the presence of the tested compounds were evaluated by Congo red assay, fluorescence and circular dichroism spectroscopy. The antioxidant (AO) capacity were also tested by four different assays including: DPPH (2,2'-diphenyl-1-picrylhydrazyl radical), ABTS (2,2-azinobis (3-ethylbenzothiazoline-6-sulphonate) diammonium salt), FRAP (ferric reducing antioxidant power) and beta-carotene-linoleic acid assay. The tested compounds showed AG and AO effects. The intensity of the accomplished AO potential is related to the type of the used assay. Significant alterations in the secondary (monitored by CD spectropolarimetry) and tertiary structure (assessed by spectrofluorimetry) of HSA upon glycation were mitigated by the 4-ACDs, suggesting their suppressive role in the late stage (post-Amadori) of the HSA glycation. Conclusions: By the analogues, in vitro ascertained AO and AG properties of 4-ACDmay be recognized as rationale for their protective role against oxidative changes of proteins, thereby precluding diabetic complications in humans.
PB  - Univ Catolica de Valparaiso, Valparaiso
T2  - Electronic Journal of Biotechnology
T1  - Antioxidant activity and protective role on protein glycation of synthetic aminocoumarins
VL  - 24
SP  - 43
EP  - 48
DO  - 10.1016/j.ejbt.2016.08.004
ER  - 

@article{
author = "Aminjafari, Akram and Miroliaei, Mehran and Angelova, Violina T. and Emamzadeh, Rahman and Đukić, Mirjana and Đurić, Ana and Saso, Luciano",
year = "2016",
abstract = "Background: Synthesized aminocoumarins are heterocyclic compounds possessing potential for the treatment of insulin-dependent diabetes mellitus with unexplored anti-glycative action. Results: In this study 4-aminocoumarin derivatives (4-ACDs) were evaluated in vitro for antiglycation (AG) activities by using the human serum albumin (HSA)/glucose system, for 8 weeks of incubation. The glycation and conformational alteration of HSA in the presence of the tested compounds were evaluated by Congo red assay, fluorescence and circular dichroism spectroscopy. The antioxidant (AO) capacity were also tested by four different assays including: DPPH (2,2'-diphenyl-1-picrylhydrazyl radical), ABTS (2,2-azinobis (3-ethylbenzothiazoline-6-sulphonate) diammonium salt), FRAP (ferric reducing antioxidant power) and beta-carotene-linoleic acid assay. The tested compounds showed AG and AO effects. The intensity of the accomplished AO potential is related to the type of the used assay. Significant alterations in the secondary (monitored by CD spectropolarimetry) and tertiary structure (assessed by spectrofluorimetry) of HSA upon glycation were mitigated by the 4-ACDs, suggesting their suppressive role in the late stage (post-Amadori) of the HSA glycation. Conclusions: By the analogues, in vitro ascertained AO and AG properties of 4-ACDmay be recognized as rationale for their protective role against oxidative changes of proteins, thereby precluding diabetic complications in humans.",
publisher = "Univ Catolica de Valparaiso, Valparaiso",
journal = "Electronic Journal of Biotechnology",
title = "Antioxidant activity and protective role on protein glycation of synthetic aminocoumarins",
volume = "24",
pages = "43-48",
doi = "10.1016/j.ejbt.2016.08.004"
}

Aminjafari, A., Miroliaei, M., Angelova, V. T., Emamzadeh, R., Đukić, M., Đurić, A.,& Saso, L.. (2016). Antioxidant activity and protective role on protein glycation of synthetic aminocoumarins. in Electronic Journal of Biotechnology
Univ Catolica de Valparaiso, Valparaiso., 24, 43-48.
https://doi.org/10.1016/j.ejbt.2016.08.004

Aminjafari A, Miroliaei M, Angelova VT, Emamzadeh R, Đukić M, Đurić A, Saso L. Antioxidant activity and protective role on protein glycation of synthetic aminocoumarins. in Electronic Journal of Biotechnology. 2016;24:43-48.
doi:10.1016/j.ejbt.2016.08.004 .

Aminjafari, Akram, Miroliaei, Mehran, Angelova, Violina T., Emamzadeh, Rahman, Đukić, Mirjana, Đurić, Ana, Saso, Luciano, "Antioxidant activity and protective role on protein glycation of synthetic aminocoumarins" in Electronic Journal of Biotechnology, 24 (2016):43-48,
https://doi.org/10.1016/j.ejbt.2016.08.004 . .