TY  - JOUR
AU  - Stojanović, Danilo
AU  - Marčetić, Mirjana
AU  - Ušjak, Dušan
AU  - Milenković, Marina
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4061
AB  - Background/Aim. Plant essential oils (EOs) can have a significant antibacterial effect especially through additive or synergistic action as antibiotic adjuvants. We investi-gated the composition and activity of EOs of two species of genus Salvia (S) from Greece with the aim to deter-mine their antimicrobial activity as well as the activity in combination with selected antibiotics. Methods. The aerial parts of wild-growing S. fruticosa and S. ringens were subjected to a steam distillation and the obtained EOs were analyzed by gas chromatography and gas chroma-tography/mass spectrometry. The broth-microdilution method was used in order to determine the minimum in-hibitory concentrations (MICs) of EOs on seven strains of bacteria and one yeast. Antimicrobial activity of the combination of EO and antibiotics was investigated by checkerboard method and estimated by calculating frac-tional inhibitory concentration (FIC) of each component and fractional inhibitory concentration index (FICI). Re-sults. Dominant component of S. fruticosa EO was trans-thujone (54.2%) and for S. ringens EO it was α-pinene (28.1%). The MICs of EOs of both species were in the range from 200 μg/mL to > 500 μg/mL. The strongest antimicrobial effect was achieved against Bacillus subtilis and Candida albicans. According to FICI values, EO of S. fruticosa had additive effect with ciprofloxacin against most of bacterial strains but not with amikacin. Conclu-sion. The essential oils of S. ringens and S. fruticosa showed modest antimicrobial activity. However, the es-sential oil of S. fruticosa showed a promising additive ef-fect in combination with ciprofloxacin.
AB  - Uvod/Cilj. Etarska ulja različitih biljaka mogu imati značajna antibakterijska svojstva, posebno kao adjuvanti antibiotika sa kojima ostvaruju aditivno ili sinergistično dejstvo. Ispitivali smo sastav i aktivnost etarskih ulja dve vrste roda Salvia (S) iz Grčke sa ciljem da odredimo njihovu antimkrobnu aktivnost, kao i dejstvo u kombi-naciji sa odabranim antibioticima. Metode. Nadzemni delovi samoniklih S. fruticosa i S. ringens su destilovani vodenom parom i dobijena etarska ulja su analizirana gasnom hromatografijom i gasnom hromatografijom sa masenom spektrometrijom. Radi određivanja minimalnih inhibitornih koncentracija (MICs) etarskog ulja na sedam sojeva bakterija i na jednoj patogenoj gljivici korišćena je mikrodiluciona metoda. Antimikrobna aktivnost kombi-nacije etarskog ulja i antibiotika ispitana je checkerboard metodom i procenjena je na osnovu frakcione inhibi-torne koncentracije (FIC) svake komponente i indeksa frakcione inhibitorne koncentracije (FICI). Resultati. Dominantna komponenta etarskog ulja S. fruticosa je bio trans-tujon (54,2%), a etarskog ulja S. ringens α-pinen (28,1%). MICs etarskog ulja obe vrste su bile u opsegu od 200 μg/mL do > 500 μg/mL. Najsnažnija anti-mikrobna aktivnost ostvarena je protiv Bacillus subtilis i Candida albicans. Na osnovu FICI vrednosti, etarsko ulje S. fruticosa je sa ciprofloksacinom, ali ne i sa amikacinom imalo aditivni efekat protiv većine bakterijskih sojeva. Zaključak. Etarska ulja S. ringens i S. fruticosa su pokazala skromnu antimikrobnu aktivnost, ali je etarsko ulje S. fru-ticosa u kombinaciji sa ciprofloksacinom ispoljilo značajan aditivni efekat.
PB  - Belgrade : Military Medical Academy, INI
T2  - Vojnosanitetski pregled
T1  - Composition and antimicrobial activity of essential oils of Salvia fruticosa and Salvia ringens (Lamiaceae)
T1  - Sastav i antimikrobna aktivnost etarskih ulja Salvia fruticosa i Salvia ringens (Lamiaceae)
VL  - 79
IS  - 1
SP  - 62
EP  - 68
DO  - 10.2298/VSP200508083S
ER  - 

@article{
author = "Stojanović, Danilo and Marčetić, Mirjana and Ušjak, Dušan and Milenković, Marina",
year = "2022",
abstract = "Background/Aim. Plant essential oils (EOs) can have a significant antibacterial effect especially through additive or synergistic action as antibiotic adjuvants. We investi-gated the composition and activity of EOs of two species of genus Salvia (S) from Greece with the aim to deter-mine their antimicrobial activity as well as the activity in combination with selected antibiotics. Methods. The aerial parts of wild-growing S. fruticosa and S. ringens were subjected to a steam distillation and the obtained EOs were analyzed by gas chromatography and gas chroma-tography/mass spectrometry. The broth-microdilution method was used in order to determine the minimum in-hibitory concentrations (MICs) of EOs on seven strains of bacteria and one yeast. Antimicrobial activity of the combination of EO and antibiotics was investigated by checkerboard method and estimated by calculating frac-tional inhibitory concentration (FIC) of each component and fractional inhibitory concentration index (FICI). Re-sults. Dominant component of S. fruticosa EO was trans-thujone (54.2%) and for S. ringens EO it was α-pinene (28.1%). The MICs of EOs of both species were in the range from 200 μg/mL to > 500 μg/mL. The strongest antimicrobial effect was achieved against Bacillus subtilis and Candida albicans. According to FICI values, EO of S. fruticosa had additive effect with ciprofloxacin against most of bacterial strains but not with amikacin. Conclu-sion. The essential oils of S. ringens and S. fruticosa showed modest antimicrobial activity. However, the es-sential oil of S. fruticosa showed a promising additive ef-fect in combination with ciprofloxacin., Uvod/Cilj. Etarska ulja različitih biljaka mogu imati značajna antibakterijska svojstva, posebno kao adjuvanti antibiotika sa kojima ostvaruju aditivno ili sinergistično dejstvo. Ispitivali smo sastav i aktivnost etarskih ulja dve vrste roda Salvia (S) iz Grčke sa ciljem da odredimo njihovu antimkrobnu aktivnost, kao i dejstvo u kombi-naciji sa odabranim antibioticima. Metode. Nadzemni delovi samoniklih S. fruticosa i S. ringens su destilovani vodenom parom i dobijena etarska ulja su analizirana gasnom hromatografijom i gasnom hromatografijom sa masenom spektrometrijom. Radi određivanja minimalnih inhibitornih koncentracija (MICs) etarskog ulja na sedam sojeva bakterija i na jednoj patogenoj gljivici korišćena je mikrodiluciona metoda. Antimikrobna aktivnost kombi-nacije etarskog ulja i antibiotika ispitana je checkerboard metodom i procenjena je na osnovu frakcione inhibi-torne koncentracije (FIC) svake komponente i indeksa frakcione inhibitorne koncentracije (FICI). Resultati. Dominantna komponenta etarskog ulja S. fruticosa je bio trans-tujon (54,2%), a etarskog ulja S. ringens α-pinen (28,1%). MICs etarskog ulja obe vrste su bile u opsegu od 200 μg/mL do > 500 μg/mL. Najsnažnija anti-mikrobna aktivnost ostvarena je protiv Bacillus subtilis i Candida albicans. Na osnovu FICI vrednosti, etarsko ulje S. fruticosa je sa ciprofloksacinom, ali ne i sa amikacinom imalo aditivni efekat protiv većine bakterijskih sojeva. Zaključak. Etarska ulja S. ringens i S. fruticosa su pokazala skromnu antimikrobnu aktivnost, ali je etarsko ulje S. fru-ticosa u kombinaciji sa ciprofloksacinom ispoljilo značajan aditivni efekat.",
publisher = "Belgrade : Military Medical Academy, INI",
journal = "Vojnosanitetski pregled",
title = "Composition and antimicrobial activity of essential oils of Salvia fruticosa and Salvia ringens (Lamiaceae), Sastav i antimikrobna aktivnost etarskih ulja Salvia fruticosa i Salvia ringens (Lamiaceae)",
volume = "79",
number = "1",
pages = "62-68",
doi = "10.2298/VSP200508083S"
}

Stojanović, D., Marčetić, M., Ušjak, D.,& Milenković, M.. (2022). Composition and antimicrobial activity of essential oils of Salvia fruticosa and Salvia ringens (Lamiaceae). in Vojnosanitetski pregled
Belgrade : Military Medical Academy, INI., 79(1), 62-68.
https://doi.org/10.2298/VSP200508083S

Stojanović D, Marčetić M, Ušjak D, Milenković M. Composition and antimicrobial activity of essential oils of Salvia fruticosa and Salvia ringens (Lamiaceae). in Vojnosanitetski pregled. 2022;79(1):62-68.
doi:10.2298/VSP200508083S .

Stojanović, Danilo, Marčetić, Mirjana, Ušjak, Dušan, Milenković, Marina, "Composition and antimicrobial activity of essential oils of Salvia fruticosa and Salvia ringens (Lamiaceae)" in Vojnosanitetski pregled, 79, no. 1 (2022):62-68,
https://doi.org/10.2298/VSP200508083S . .

TY  - CONF
AU  - Milutinović, Violeta
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4449
AB  - The hawkweed genus Hieracium L. s. str. (Asteraceae) is one of the species-richest and
systematically the most complex genera of flowering plants. The genus traditional
classification, beside Hieracium, included, among others, subgenus Pilosella, which is
currently considered as separate genus. In folk medicine, application of some Hieracium s.
str. species was reported for various respiratory, gastrointestinal, urogenital, and some skin
disorders. The 28 species from the Balkan Peninsula were investigated, and majority was
collected on the Mt Durmitor (Montenegro), where the greatest diversity of taxa of this genus
was registered. In flowering aerial parts, composition of pharmaceutically significant
secondary metabolites - flavonoids, phenolic acids, sesquiterpene lactones and triterpenes
was established, and by multivariate statistical analysis their chemosystematic significance
was demonstrated. Corresponding metabolites isolation was performed using column
chromatography and semi-preparative liquid chromatography (LC-MS), identification using
mass spectrometry, NMR spectroscopy and refractometry, and extracts comparative
qualitative and quantitative analysis using LC-MS or gas chromatography (GC-FID-MS). In all
28 species, sesquiterpene lactones were identified for the first time. Three of them were
previously undescribed proline conjugates. Sesquiterpene lactone-amino acid conjugates are
quite rare in nature, and formerly were identified only in a few species of other Asteraceae
genera. Results obtained by investigation of pharmacological activities (in vitro antioxidant,
antimicrobial, cytotoxic, anticholinesterase, and in vivo antihyperalgesic and antiedematous),
as well as safety profiles of selected, chemically characterized extracts and/or secondary
metabolites, indicated the medicinal potential of the analysed plants and gave justification
for further research aiming to find new natural medicinal raw materials.
AB  - Rod Hieracium L. s. str. (jastrebnik, runjavica) (Asteraceae) je jedan od najvećih i
sistematski najsloženijih rodova skrivenosemenica. Tradicionalna klasifikacija roda je pored
podroda Hieracium uključivala, između ostalog, i podrod Pilosella, koji prema savremenoj
klasifikaciji ima status roda. Za pojedine vrste roda Hieracium s. str. je zabeležena primena u
narodnoj medicini kod različitih poremećaja respiratornog, gastrointestinalnog, urogenitalnog
trakta i kod nekih kožnih bolesti. Sprovedeno je istraživanje 28 vrsta sa Balkanskog
poluostrva, od kojih je najveć i broj sakupljen na planini Durmitor (Crna Gora), na kojoj je
konstatovan najveći diverzitet taksona roda. U nadzemnim delovima biljaka u cvetu ustanovljena
je kompozicija sekundarnih metabolita od značaja za farmaciju - flavonoida, fenolkarboksilnih
kiselina, seskviterpenskih laktona i triterpena, i multivarijantnom statističkom analizom
pokazan i njihov hemosistematski značaj. Izolovanje odgovarajućih metabolita izvršeno je
primenom hromatografije u koloni i semi-preparativne tečne hromatografije (LC-MS),
identifikacija primenom masene spektrometrije, NMR spektroskopije i refraktometrije, a uporedna
kvalitativna i kvantitativna analiza ekstrakata primenom LC-MS ili gasne hromatografije (GC-FID-
MS). Seskviterpenski laktoni su u svih 28 vrsta identifikovani po prvi put, od kojih su tri do
sada neopisani konjugati sa prolinom. Konjugati seskviterpenskih laktona sa
aminokiselinama su inače vrlo retki u prirodi i do sada su identifikovani samo u nekoliko
vrsta drugih rodova familije Asteraceae. Rezultati dobijeni ispitivanjem farmakološke
aktivnosti (in vitro antioksidantne, antimikrobne, citotoksične, antiholinesterazne, i in vivo
antihiperalgezijske i antiedematozne), kao i rezultati ispitivanja bezbednosti primene za
odabrane, hemijski okarakterisane ekstrakte i/ili sekundarne metabolite ukazali su na
lekoviti potencijal ispitivanih biljaka i opravdanost daljih istraživanja u cilju iznalaženja
novih prirodnih lekovitih sirovina.
PB  - Savez farmaceutskih udruženja Srbije (SFUS)
C3  - Arhiv za farmaciju
T1  - Hieracium s. str. species from the Balkan Peninsula: secondary metabolites and pharmacological potential
T1  - Vrste roda Hieracium s. str. sa Balkanskog poluostrva: sekundarni metaboliti i farmakološki potencijal
VL  - 72
IS  - 4 suplement
SP  - S64
EP  - S65
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4449
ER  - 

@conference{
author = "Milutinović, Violeta",
year = "2022",
abstract = "The hawkweed genus Hieracium L. s. str. (Asteraceae) is one of the species-richest and
systematically the most complex genera of flowering plants. The genus traditional
classification, beside Hieracium, included, among others, subgenus Pilosella, which is
currently considered as separate genus. In folk medicine, application of some Hieracium s.
str. species was reported for various respiratory, gastrointestinal, urogenital, and some skin
disorders. The 28 species from the Balkan Peninsula were investigated, and majority was
collected on the Mt Durmitor (Montenegro), where the greatest diversity of taxa of this genus
was registered. In flowering aerial parts, composition of pharmaceutically significant
secondary metabolites - flavonoids, phenolic acids, sesquiterpene lactones and triterpenes
was established, and by multivariate statistical analysis their chemosystematic significance
was demonstrated. Corresponding metabolites isolation was performed using column
chromatography and semi-preparative liquid chromatography (LC-MS), identification using
mass spectrometry, NMR spectroscopy and refractometry, and extracts comparative
qualitative and quantitative analysis using LC-MS or gas chromatography (GC-FID-MS). In all
28 species, sesquiterpene lactones were identified for the first time. Three of them were
previously undescribed proline conjugates. Sesquiterpene lactone-amino acid conjugates are
quite rare in nature, and formerly were identified only in a few species of other Asteraceae
genera. Results obtained by investigation of pharmacological activities (in vitro antioxidant,
antimicrobial, cytotoxic, anticholinesterase, and in vivo antihyperalgesic and antiedematous),
as well as safety profiles of selected, chemically characterized extracts and/or secondary
metabolites, indicated the medicinal potential of the analysed plants and gave justification
for further research aiming to find new natural medicinal raw materials., Rod Hieracium L. s. str. (jastrebnik, runjavica) (Asteraceae) je jedan od najvećih i
sistematski najsloženijih rodova skrivenosemenica. Tradicionalna klasifikacija roda je pored
podroda Hieracium uključivala, između ostalog, i podrod Pilosella, koji prema savremenoj
klasifikaciji ima status roda. Za pojedine vrste roda Hieracium s. str. je zabeležena primena u
narodnoj medicini kod različitih poremećaja respiratornog, gastrointestinalnog, urogenitalnog
trakta i kod nekih kožnih bolesti. Sprovedeno je istraživanje 28 vrsta sa Balkanskog
poluostrva, od kojih je najveć i broj sakupljen na planini Durmitor (Crna Gora), na kojoj je
konstatovan najveći diverzitet taksona roda. U nadzemnim delovima biljaka u cvetu ustanovljena
je kompozicija sekundarnih metabolita od značaja za farmaciju - flavonoida, fenolkarboksilnih
kiselina, seskviterpenskih laktona i triterpena, i multivarijantnom statističkom analizom
pokazan i njihov hemosistematski značaj. Izolovanje odgovarajućih metabolita izvršeno je
primenom hromatografije u koloni i semi-preparativne tečne hromatografije (LC-MS),
identifikacija primenom masene spektrometrije, NMR spektroskopije i refraktometrije, a uporedna
kvalitativna i kvantitativna analiza ekstrakata primenom LC-MS ili gasne hromatografije (GC-FID-
MS). Seskviterpenski laktoni su u svih 28 vrsta identifikovani po prvi put, od kojih su tri do
sada neopisani konjugati sa prolinom. Konjugati seskviterpenskih laktona sa
aminokiselinama su inače vrlo retki u prirodi i do sada su identifikovani samo u nekoliko
vrsta drugih rodova familije Asteraceae. Rezultati dobijeni ispitivanjem farmakološke
aktivnosti (in vitro antioksidantne, antimikrobne, citotoksične, antiholinesterazne, i in vivo
antihiperalgezijske i antiedematozne), kao i rezultati ispitivanja bezbednosti primene za
odabrane, hemijski okarakterisane ekstrakte i/ili sekundarne metabolite ukazali su na
lekoviti potencijal ispitivanih biljaka i opravdanost daljih istraživanja u cilju iznalaženja
novih prirodnih lekovitih sirovina.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "Hieracium s. str. species from the Balkan Peninsula: secondary metabolites and pharmacological potential, Vrste roda Hieracium s. str. sa Balkanskog poluostrva: sekundarni metaboliti i farmakološki potencijal",
volume = "72",
number = "4 suplement",
pages = "S64-S65",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4449"
}

Milutinović, V.. (2022). Hieracium s. str. species from the Balkan Peninsula: secondary metabolites and pharmacological potential. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije (SFUS)., 72(4 suplement), S64-S65.
https://hdl.handle.net/21.15107/rcub_farfar_4449

Milutinović V. Hieracium s. str. species from the Balkan Peninsula: secondary metabolites and pharmacological potential. in Arhiv za farmaciju. 2022;72(4 suplement):S64-S65.
https://hdl.handle.net/21.15107/rcub_farfar_4449 .

Milutinović, Violeta, "Hieracium s. str. species from the Balkan Peninsula: secondary metabolites and pharmacological potential" in Arhiv za farmaciju, 72, no. 4 suplement (2022):S64-S65,
https://hdl.handle.net/21.15107/rcub_farfar_4449 .

TY  - CONF
AU  - Ušjak, Ljuboš
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4448
AB  - Significance of pharmacognostic studies on previously untested or scarcely tested
wild plants is constantly rising, considering growing demand of pharmaceutical industry for
new bioactive isolates, including essential oils and extracts. Accordingly, comprehensive
research that included all nine taxa of genus Heracleum L. (Apiaceae) (three of which are
endemic) from Serbia, Montenegro, North Macedonia and Slovenia was performed (60
samples of various plant organs collected from 14 localities in period 2009-2016). Plants of
this genus (cow parsnips) got their Latin name after ancient hero Heracles, because of their
habitus and use in folk medicine in treatment of various diseases. Also, some of them were
the main ingredient of borscht, a traditional dish in Eastern European countries. They are
interesting because of wide range of metabolites: from essential oil components, coumarins,
flavonoids, phenolic acids, triterpenes and phytosterols, to some specific fatty acids. Analysis
of these secondary and primary metabolites was performed using modern chromatographic
and spectroscopic techniques (GC-FID-MS, LC-MS and NMR). In the case of 35 selected
essential oils, antimicrobial, cytotoxic and/or antioxidant activities were also tested. It was
revealed that studied plants represent potential new sources of compounds of importance
not only for pharmaceutical, but also for cosmetic, food and chemical industries. For
components of essential oils and furanocoumarins, by multivariate statistical analysis,
chemosystematic significance was also established. Results of pharmacological
investigations of essential oils indicated their importance as potential new medicinal plant
raw materials, under condition of respecting established maximum daily intakes for those
oils in which furanocoumarins were detected.
AB  - Značaj farmakognozijskih studija na do sada neispitivanim ili nedovoljno ispitivanim
samoniklim biljkama konstantno raste s obzirom na sve veće potrebe farmaceutske
industrije za novim bioaktivnim izolatima, uključujući i etarska ulja i ekstrakte. U skladu sa
tim, sprovedeno je opsežno istraživanje svih devet taksona roda Heracleum L. (Apiaceae) (od
kojih su tri endemična), sa područja Srbije, Crne Gore, Severne Makedonije i Slovenije (60
uzoraka različitih biljnih organa sakupljanih sa 14 lokaliteta u periodu od 2009-2016. god.).
Biljke ovog roda (mečje šape) dobile su latinski naziv po antičkom heroju Heraklu, kako zbog
svog habitusa, tako i zbog primene u narodnoj medicini u lečenju različitih bolesti. Takođe,
neke od njih bile su glavni sastojak boršča, tradicionalnog jela u zemljama istočne Evrope.
Interesantne su zbog širokog spektra metabolita: od komponenti etarskih ulja, kumarina,
flavonoida, fenolkarboksilnih kiselina, triterpena i fitosterola, sve do nekih specifičnih
masnih kiselina. Ispitivanje navedenih sekundarnih i primarnih metabolita ovih taksona
sprovedeno je uz korišćenje savremenih hromatografskih i spektroskopskih tehnika (GC-
FID-MS, LC-MS i NMR). U slučaju 35 odabranih etarskih ulja ispitivane su i antimikrobna,
citotoksična i/ili antioksidantna aktivnost. Utvrđeno je da su proučavane biljke potencijalno
novi izvori jedinjenja od značaja ne samo za farmaceutsku, već i za kozmetičku,
prehrambenu i hemijsku industriju. Za komponente etarskih ulja i furanokumarine
ustanovljeno je multivarijantnom statističkom analizom da imaju i hemosistematski značaj.
Rezultati ispitivanja farmakoloških aktivnosti etarskih ulja ukazali su na njihovu važnost kao
potencijalno novih lekovitih biljnih sirovina, uz uslov poštovanja ustanovljenih maksimalnih
dozvoljenih dnevnih unosa za ona etarska ulja u kojima su detektovani furanokumarini.
PB  - Savez farmaceutskih udruženja Srbije (SFUS)
C3  - Arhiv za farmaciju
T1  - Investigations of plants of the genus Heracleum autochthonous for Southeastern Europe - searching for new natural medicinal raw materials
T1  - Ispitivanja biljaka roda heracleum autohtonih za jugoistočnu evropu ‐ u potrazi za novim prirodnim lekovitim sirovinama
VL  - 72
IS  - 4 suplement
SP  - S62
EP  - S63
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4448
ER  - 

@conference{
author = "Ušjak, Ljuboš",
year = "2022",
abstract = "Significance of pharmacognostic studies on previously untested or scarcely tested
wild plants is constantly rising, considering growing demand of pharmaceutical industry for
new bioactive isolates, including essential oils and extracts. Accordingly, comprehensive
research that included all nine taxa of genus Heracleum L. (Apiaceae) (three of which are
endemic) from Serbia, Montenegro, North Macedonia and Slovenia was performed (60
samples of various plant organs collected from 14 localities in period 2009-2016). Plants of
this genus (cow parsnips) got their Latin name after ancient hero Heracles, because of their
habitus and use in folk medicine in treatment of various diseases. Also, some of them were
the main ingredient of borscht, a traditional dish in Eastern European countries. They are
interesting because of wide range of metabolites: from essential oil components, coumarins,
flavonoids, phenolic acids, triterpenes and phytosterols, to some specific fatty acids. Analysis
of these secondary and primary metabolites was performed using modern chromatographic
and spectroscopic techniques (GC-FID-MS, LC-MS and NMR). In the case of 35 selected
essential oils, antimicrobial, cytotoxic and/or antioxidant activities were also tested. It was
revealed that studied plants represent potential new sources of compounds of importance
not only for pharmaceutical, but also for cosmetic, food and chemical industries. For
components of essential oils and furanocoumarins, by multivariate statistical analysis,
chemosystematic significance was also established. Results of pharmacological
investigations of essential oils indicated their importance as potential new medicinal plant
raw materials, under condition of respecting established maximum daily intakes for those
oils in which furanocoumarins were detected., Značaj farmakognozijskih studija na do sada neispitivanim ili nedovoljno ispitivanim
samoniklim biljkama konstantno raste s obzirom na sve veće potrebe farmaceutske
industrije za novim bioaktivnim izolatima, uključujući i etarska ulja i ekstrakte. U skladu sa
tim, sprovedeno je opsežno istraživanje svih devet taksona roda Heracleum L. (Apiaceae) (od
kojih su tri endemična), sa područja Srbije, Crne Gore, Severne Makedonije i Slovenije (60
uzoraka različitih biljnih organa sakupljanih sa 14 lokaliteta u periodu od 2009-2016. god.).
Biljke ovog roda (mečje šape) dobile su latinski naziv po antičkom heroju Heraklu, kako zbog
svog habitusa, tako i zbog primene u narodnoj medicini u lečenju različitih bolesti. Takođe,
neke od njih bile su glavni sastojak boršča, tradicionalnog jela u zemljama istočne Evrope.
Interesantne su zbog širokog spektra metabolita: od komponenti etarskih ulja, kumarina,
flavonoida, fenolkarboksilnih kiselina, triterpena i fitosterola, sve do nekih specifičnih
masnih kiselina. Ispitivanje navedenih sekundarnih i primarnih metabolita ovih taksona
sprovedeno je uz korišćenje savremenih hromatografskih i spektroskopskih tehnika (GC-
FID-MS, LC-MS i NMR). U slučaju 35 odabranih etarskih ulja ispitivane su i antimikrobna,
citotoksična i/ili antioksidantna aktivnost. Utvrđeno je da su proučavane biljke potencijalno
novi izvori jedinjenja od značaja ne samo za farmaceutsku, već i za kozmetičku,
prehrambenu i hemijsku industriju. Za komponente etarskih ulja i furanokumarine
ustanovljeno je multivarijantnom statističkom analizom da imaju i hemosistematski značaj.
Rezultati ispitivanja farmakoloških aktivnosti etarskih ulja ukazali su na njihovu važnost kao
potencijalno novih lekovitih biljnih sirovina, uz uslov poštovanja ustanovljenih maksimalnih
dozvoljenih dnevnih unosa za ona etarska ulja u kojima su detektovani furanokumarini.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "Investigations of plants of the genus Heracleum autochthonous for Southeastern Europe - searching for new natural medicinal raw materials, Ispitivanja biljaka roda heracleum autohtonih za jugoistočnu evropu ‐ u potrazi za novim prirodnim lekovitim sirovinama",
volume = "72",
number = "4 suplement",
pages = "S62-S63",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4448"
}

Ušjak, L.. (2022). Investigations of plants of the genus Heracleum autochthonous for Southeastern Europe - searching for new natural medicinal raw materials. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije (SFUS)., 72(4 suplement), S62-S63.
https://hdl.handle.net/21.15107/rcub_farfar_4448

Ušjak L. Investigations of plants of the genus Heracleum autochthonous for Southeastern Europe - searching for new natural medicinal raw materials. in Arhiv za farmaciju. 2022;72(4 suplement):S62-S63.
https://hdl.handle.net/21.15107/rcub_farfar_4448 .

Ušjak, Ljuboš, "Investigations of plants of the genus Heracleum autochthonous for Southeastern Europe - searching for new natural medicinal raw materials" in Arhiv za farmaciju, 72, no. 4 suplement (2022):S62-S63,
https://hdl.handle.net/21.15107/rcub_farfar_4448 .

TY  - JOUR
AU  - Suručić, Relja
AU  - Kundaković-Vasović, Tatjana
AU  - Marčetić, Mirjana
AU  - Drakul, Dragana
AU  - Milenković, Marina
AU  - Kovačević, Nada
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4002
AB  - Phytochemical and pharmacological in-vestigations of essential oils isolated from plant species of the genus Seseli have been intensified recently. These plant species have long-term use in nutrition and traditional medicine in the treatment of various disorders. Volatile secondary metabolites of Seseli gracile Waldst. & Kit (Apiaceae) have not been pharma-cologically examined so far. The aim of the conducted research was to assess the antiradical, antimicrobial and spasmolytic ac-tivities of S. gracile essential oil isolated from the aerial parts of the plant. Methods. The antiradical activity was determined us-ing the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scaveng-ing test, antimicrobial activity using broth microdilution meth-od with standard strains of Gram (+), Gram (-) bacteria and yeast, while spasmolytic activity was evaluated on isolated rat il-eum, pre-contracted with a high concentration of potassium. Results. The results showed moderate antiradical (SC50 = 78.60 μg/mL) and good spasmolytic activity (IC50 = 271.4 nL/mL) of S. gracile essential oil. In the range of tested concen-trations, minimal inhibitory concentration (MIC) was deter-mined only for the strain of Escherichia coli (500 μg/mL). Con-clusion. Results obtained in this study justify the need for fur-ther studies to elucidate exact molecular mechanism underlay this spasmolytic effect of S. gracile herb essential oil.
AB  - Etarska ulja izolovana iz vrsta roda Seseli su u poslednje vreme intenzivno farmakološki i fitohemijski proučavana. Biljke iz ovog roda se koriste već duži niz godina u ishrani i tradicionalnoj terapiji različitih oboljenja. Farmakološka aktivnost etarskog ulja izolovanog iz Seseli gracile do sada nije ispitivana. Stoga je cilj ovog istraživanja bio da se ispita antiradikalska, antimikrobna i spazmolitična aktivnost etarskog ulja izolovanog iz nadzemnih delova biljne vrste S. gracile. Metode. Antiradikalska aktivnost je utvrđena testom neutralizacije 2.2-difenil-1-pikrilhidrazil (DPPH) radikala, antimikrobna, bujon-mikrodilucionom metodom uz upotrebu standardnih sojeva Gram (+), Gram (-) bakterija i gljivica, a spazmolitična na izolovanom ileumu pacova, prethodno kontrahovanom visokom koncentarcijom kalijuma. Rezultati. Rezultati pokazuju umerenu antiradikalsku (SC50 = 78.60 μg/mL) i dobru spazmolitičnu aktivnost (IC50 = 271.4 nL/mL) etarskog ulja S. gracile. U opsegu testiranih koncentracija utvrđena je minimalna inhibitorna koncentracija (MIC) samo za soj Escherichia coli (500 μg/mL). Zaključak. Dobijeni rezultati opravdavaju potrebu za budućim istraživanjima koja bi razjasnila tačne mehanizme kojima se ostvaruje spazmolitički efekat etarskog ulja nadzemnog dela S. gracile.
PB  - Belgrade : Military Medical Academy, INI
T2  - Vojnosanitetski pregled
T1  - Pharmacological characterisation of Seseli gracile Waldst. & Kit. (Apiaceae) essential oil
T1  - Farmakološka karakterizacija etarskog ulja Seseli gracile Waldst. & Kit. (Apiaceae)
VL  - 78
IS  - 10
SP  - 1065
EP  - 1071
DO  - 10.2298/VSP190320028S
ER  - 

@article{
author = "Suručić, Relja and Kundaković-Vasović, Tatjana and Marčetić, Mirjana and Drakul, Dragana and Milenković, Marina and Kovačević, Nada",
year = "2021",
abstract = "Phytochemical and pharmacological in-vestigations of essential oils isolated from plant species of the genus Seseli have been intensified recently. These plant species have long-term use in nutrition and traditional medicine in the treatment of various disorders. Volatile secondary metabolites of Seseli gracile Waldst. & Kit (Apiaceae) have not been pharma-cologically examined so far. The aim of the conducted research was to assess the antiradical, antimicrobial and spasmolytic ac-tivities of S. gracile essential oil isolated from the aerial parts of the plant. Methods. The antiradical activity was determined us-ing the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scaveng-ing test, antimicrobial activity using broth microdilution meth-od with standard strains of Gram (+), Gram (-) bacteria and yeast, while spasmolytic activity was evaluated on isolated rat il-eum, pre-contracted with a high concentration of potassium. Results. The results showed moderate antiradical (SC50 = 78.60 μg/mL) and good spasmolytic activity (IC50 = 271.4 nL/mL) of S. gracile essential oil. In the range of tested concen-trations, minimal inhibitory concentration (MIC) was deter-mined only for the strain of Escherichia coli (500 μg/mL). Con-clusion. Results obtained in this study justify the need for fur-ther studies to elucidate exact molecular mechanism underlay this spasmolytic effect of S. gracile herb essential oil., Etarska ulja izolovana iz vrsta roda Seseli su u poslednje vreme intenzivno farmakološki i fitohemijski proučavana. Biljke iz ovog roda se koriste već duži niz godina u ishrani i tradicionalnoj terapiji različitih oboljenja. Farmakološka aktivnost etarskog ulja izolovanog iz Seseli gracile do sada nije ispitivana. Stoga je cilj ovog istraživanja bio da se ispita antiradikalska, antimikrobna i spazmolitična aktivnost etarskog ulja izolovanog iz nadzemnih delova biljne vrste S. gracile. Metode. Antiradikalska aktivnost je utvrđena testom neutralizacije 2.2-difenil-1-pikrilhidrazil (DPPH) radikala, antimikrobna, bujon-mikrodilucionom metodom uz upotrebu standardnih sojeva Gram (+), Gram (-) bakterija i gljivica, a spazmolitična na izolovanom ileumu pacova, prethodno kontrahovanom visokom koncentarcijom kalijuma. Rezultati. Rezultati pokazuju umerenu antiradikalsku (SC50 = 78.60 μg/mL) i dobru spazmolitičnu aktivnost (IC50 = 271.4 nL/mL) etarskog ulja S. gracile. U opsegu testiranih koncentracija utvrđena je minimalna inhibitorna koncentracija (MIC) samo za soj Escherichia coli (500 μg/mL). Zaključak. Dobijeni rezultati opravdavaju potrebu za budućim istraživanjima koja bi razjasnila tačne mehanizme kojima se ostvaruje spazmolitički efekat etarskog ulja nadzemnog dela S. gracile.",
publisher = "Belgrade : Military Medical Academy, INI",
journal = "Vojnosanitetski pregled",
title = "Pharmacological characterisation of Seseli gracile Waldst. & Kit. (Apiaceae) essential oil, Farmakološka karakterizacija etarskog ulja Seseli gracile Waldst. & Kit. (Apiaceae)",
volume = "78",
number = "10",
pages = "1065-1071",
doi = "10.2298/VSP190320028S"
}

Suručić, R., Kundaković-Vasović, T., Marčetić, M., Drakul, D., Milenković, M.,& Kovačević, N.. (2021). Pharmacological characterisation of Seseli gracile Waldst. & Kit. (Apiaceae) essential oil. in Vojnosanitetski pregled
Belgrade : Military Medical Academy, INI., 78(10), 1065-1071.
https://doi.org/10.2298/VSP190320028S

Suručić R, Kundaković-Vasović T, Marčetić M, Drakul D, Milenković M, Kovačević N. Pharmacological characterisation of Seseli gracile Waldst. & Kit. (Apiaceae) essential oil. in Vojnosanitetski pregled. 2021;78(10):1065-1071.
doi:10.2298/VSP190320028S .

Suručić, Relja, Kundaković-Vasović, Tatjana, Marčetić, Mirjana, Drakul, Dragana, Milenković, Marina, Kovačević, Nada, "Pharmacological characterisation of Seseli gracile Waldst. & Kit. (Apiaceae) essential oil" in Vojnosanitetski pregled, 78, no. 10 (2021):1065-1071,
https://doi.org/10.2298/VSP190320028S . .

TY  - THES
AU  - Milutinović, Violeta
PY  - 2021
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=8442
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:24744/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=49042953
UR  - https://nardus.mpn.gov.rs/handle/123456789/18845
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4039
AB  - hibridogenih).Semipreparativnim LC-MS metodama, iz MeOH ekstrakta cvasti H. calophyllum izolovana sučetiri seskviterpenska laktona (krepizid E i tri nova jedinjenja: 8-epi-ikserisamin A, kalofilamin A ikalofilamin B), a iz MeOH ekstrakta podzemnih organa H. scheppigianum pet fenolkarboksilnih(mono- ili di-O-kafeoilhina) kiselina (identifikacija na osnovu NMR i MS spektara).Ukupno četiri seskviterpenska laktona, sedam mono- ili di-O-kafeoilhina kiselina i 19flavonoida (luteolin, apigenin, diosmetin, 15 njihovih heterozida i heterozid kvercetina)kvantifikovani su u suvim MeOH ekstraktima ispitivanih podzemnih organa dve vrste i herbi 28vrsta LC-MS metodama, i pet triterpena (derivata ursana, oleana i lupana) u suvim CH2Cl2ekstraktima herbi 28 vrsta GC-FID-MS metodom (identifikacija pomoću standarda, NMR, MS i/iliUV spektara). Hemosistematski značaj jedinjenja identifikovanih u herbama 28 vrsta utvrđen jemultivarijantnim statističkim metodama (PCA, nMDS i UPGMA).Suvi MeOH ekstrakti herbi su in vitro pokazali značajan ukupni antioksidantni potencijal isposobnost neutralizacije slobodnih DPPH•, ABTS•+ i OH• radikala (28 vrsta) i inhibicijeacetilholinesteraze (27 vrsta) i butirilholinesteraze (7 vrsta). Među testiranih 12 jedinjenja, najboljuantiholinesteraznu aktivnost ispoljila su tri flavonoidna aglikona i 8-epi-ikserisamin A. Odabranisuvi MeOH i CH2Cl2 ekstrakti herbi šest vrsta pokazali su antimikrobnu aktivnost prema 18, a 8-epi-ikserisamin A i krepizid E prema 4 mikroorganizma. Isti odabrani izolati ispoljili sucitotoksični efekat prema tri, odnosno jednoj tumorskoj ćelijskoj liniji, uz zanemarljivu inhibicijurasta zdravih ćelija. Tri odabrana MeOH i CH2Cl2 ekstrakta su u modelu inflamacije šape pacovaizazvane karageninom pokazala antihiperalgezijsku, ali ne i antiedematoznu aktivnost; bezbednostprimene dva ekstrakta koji su ispoljili značajnu antihiperalgezijsku aktivnost pokazana je in vivorotarod i testom akutne toksičnosti.Ključne reči:
AB  - nonhybridogenousand 16 hybridogenous).Using semipreparative LC-MS methods, four sesquiterpene lactones (crepiside E and three newcompounds: 8-epiixerisamine A, calophyllamine A and calophyllamine B) were isolated from theMeOH extract of H. calophyllum flowering heads, and five phenolic (mono- or di-Ocaffeoylquinic)acids from the MeOH extract of H. scheppigianum underground parts(identification based on NMR and MS spectra).Total of four sesquiterpene lactones, seven mono- or di-O-caffeoylquinic acids and 19flavonoids (luteolin, apigenin, diosmetin, their 15 glycosides and one quercetin glycoside) werequantified in the dried MeOH extracts of the investigated underground parts of two species andherbs of 28 species, using LC-MS methods, and five triterpenes (ursane, oleane and lupanederivatives) in the dried CH2Cl2 extracts of the herbs of 28 species, using GC-FID-MS method(identification using standards, NMR, MS and/or UV spectra). Chemosystematic significance ofthe compounds identified in the herbs of 28 species was determined using multivariate statistics(PCA, nMDS and UPGMA).Dried MeOH herb extracts in vitro exhibited significant total antioxidant activity andscavenging of DPPH•, ABTS•+ and OH• radicals (28 species), as well as the ability to inhibitacetylcholinesterase (27 species) and butyrylcholinesterase (7 species). Among 12 testedcompounds, three flavonoid aglycones and 8-epiixerisamine A exhibited the highestanticholinesterase activity. Selected dried MeOH and CH2Cl2 herb extracts of six species showedantimicrobial activity against 18, whereas 8-epiixerisamine A and crepiside E against fourmicroorganisms. The same selected isolates exhibited cytotoxic activity against three and onecancer cell lines, respectively, with negligible inhibition of the normal cells growth. In thecarrageenan-induced localized inflammation model in rats, three selected MeOH and CH2Cl2extracts exhibited antihyperalgesic, but not antiedematous activity; safety of the oral administrationof two extracts that exhibited significant antihyperalgesic activity was demonstrated in vivo inrotarod test and acute oral toxicity study.
PB  - Универзитет у Београду, Фармацеутски факултет
T2  - Универзитет у Београду
T1  - Ispitivanje sekundarnih metabolita i farmakološke aktivnosti odabranih vrsta roda Hieracium L. (Asteraceae) sa Balkanskog poluostrva
UR  - https://hdl.handle.net/21.15107/rcub_nardus_18845
ER  - 

@phdthesis{
author = "Milutinović, Violeta",
year = "2021",
abstract = "hibridogenih).Semipreparativnim LC-MS metodama, iz MeOH ekstrakta cvasti H. calophyllum izolovana sučetiri seskviterpenska laktona (krepizid E i tri nova jedinjenja: 8-epi-ikserisamin A, kalofilamin A ikalofilamin B), a iz MeOH ekstrakta podzemnih organa H. scheppigianum pet fenolkarboksilnih(mono- ili di-O-kafeoilhina) kiselina (identifikacija na osnovu NMR i MS spektara).Ukupno četiri seskviterpenska laktona, sedam mono- ili di-O-kafeoilhina kiselina i 19flavonoida (luteolin, apigenin, diosmetin, 15 njihovih heterozida i heterozid kvercetina)kvantifikovani su u suvim MeOH ekstraktima ispitivanih podzemnih organa dve vrste i herbi 28vrsta LC-MS metodama, i pet triterpena (derivata ursana, oleana i lupana) u suvim CH2Cl2ekstraktima herbi 28 vrsta GC-FID-MS metodom (identifikacija pomoću standarda, NMR, MS i/iliUV spektara). Hemosistematski značaj jedinjenja identifikovanih u herbama 28 vrsta utvrđen jemultivarijantnim statističkim metodama (PCA, nMDS i UPGMA).Suvi MeOH ekstrakti herbi su in vitro pokazali značajan ukupni antioksidantni potencijal isposobnost neutralizacije slobodnih DPPH•, ABTS•+ i OH• radikala (28 vrsta) i inhibicijeacetilholinesteraze (27 vrsta) i butirilholinesteraze (7 vrsta). Među testiranih 12 jedinjenja, najboljuantiholinesteraznu aktivnost ispoljila su tri flavonoidna aglikona i 8-epi-ikserisamin A. Odabranisuvi MeOH i CH2Cl2 ekstrakti herbi šest vrsta pokazali su antimikrobnu aktivnost prema 18, a 8-epi-ikserisamin A i krepizid E prema 4 mikroorganizma. Isti odabrani izolati ispoljili sucitotoksični efekat prema tri, odnosno jednoj tumorskoj ćelijskoj liniji, uz zanemarljivu inhibicijurasta zdravih ćelija. Tri odabrana MeOH i CH2Cl2 ekstrakta su u modelu inflamacije šape pacovaizazvane karageninom pokazala antihiperalgezijsku, ali ne i antiedematoznu aktivnost; bezbednostprimene dva ekstrakta koji su ispoljili značajnu antihiperalgezijsku aktivnost pokazana je in vivorotarod i testom akutne toksičnosti.Ključne reči:, nonhybridogenousand 16 hybridogenous).Using semipreparative LC-MS methods, four sesquiterpene lactones (crepiside E and three newcompounds: 8-epiixerisamine A, calophyllamine A and calophyllamine B) were isolated from theMeOH extract of H. calophyllum flowering heads, and five phenolic (mono- or di-Ocaffeoylquinic)acids from the MeOH extract of H. scheppigianum underground parts(identification based on NMR and MS spectra).Total of four sesquiterpene lactones, seven mono- or di-O-caffeoylquinic acids and 19flavonoids (luteolin, apigenin, diosmetin, their 15 glycosides and one quercetin glycoside) werequantified in the dried MeOH extracts of the investigated underground parts of two species andherbs of 28 species, using LC-MS methods, and five triterpenes (ursane, oleane and lupanederivatives) in the dried CH2Cl2 extracts of the herbs of 28 species, using GC-FID-MS method(identification using standards, NMR, MS and/or UV spectra). Chemosystematic significance ofthe compounds identified in the herbs of 28 species was determined using multivariate statistics(PCA, nMDS and UPGMA).Dried MeOH herb extracts in vitro exhibited significant total antioxidant activity andscavenging of DPPH•, ABTS•+ and OH• radicals (28 species), as well as the ability to inhibitacetylcholinesterase (27 species) and butyrylcholinesterase (7 species). Among 12 testedcompounds, three flavonoid aglycones and 8-epiixerisamine A exhibited the highestanticholinesterase activity. Selected dried MeOH and CH2Cl2 herb extracts of six species showedantimicrobial activity against 18, whereas 8-epiixerisamine A and crepiside E against fourmicroorganisms. The same selected isolates exhibited cytotoxic activity against three and onecancer cell lines, respectively, with negligible inhibition of the normal cells growth. In thecarrageenan-induced localized inflammation model in rats, three selected MeOH and CH2Cl2extracts exhibited antihyperalgesic, but not antiedematous activity; safety of the oral administrationof two extracts that exhibited significant antihyperalgesic activity was demonstrated in vivo inrotarod test and acute oral toxicity study.",
publisher = "Универзитет у Београду, Фармацеутски факултет",
journal = "Универзитет у Београду",
title = "Ispitivanje sekundarnih metabolita i farmakološke aktivnosti odabranih vrsta roda Hieracium L. (Asteraceae) sa Balkanskog poluostrva",
url = "https://hdl.handle.net/21.15107/rcub_nardus_18845"
}

Milutinović, V.. (2021). Ispitivanje sekundarnih metabolita i farmakološke aktivnosti odabranih vrsta roda Hieracium L. (Asteraceae) sa Balkanskog poluostrva. in Универзитет у Београду
Универзитет у Београду, Фармацеутски факултет..
https://hdl.handle.net/21.15107/rcub_nardus_18845

Milutinović V. Ispitivanje sekundarnih metabolita i farmakološke aktivnosti odabranih vrsta roda Hieracium L. (Asteraceae) sa Balkanskog poluostrva. in Универзитет у Београду. 2021;.
https://hdl.handle.net/21.15107/rcub_nardus_18845 .

Milutinović, Violeta, "Ispitivanje sekundarnih metabolita i farmakološke aktivnosti odabranih vrsta roda Hieracium L. (Asteraceae) sa Balkanskog poluostrva" in Универзитет у Београду (2021),
https://hdl.handle.net/21.15107/rcub_nardus_18845 .

TY  - JOUR
AU  - Milutinović, Violeta
AU  - Pecikoza, Uroš
AU  - Tomić, Maja
AU  - Stepanović-Petrović, Radica
AU  - Niketić, Marjan
AU  - Ušjak, Ljuboš
AU  - Petrović, Silvana
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3641
AB  - Present study investigated triterpene profile, antihyperalgesic and antiedematous activities of Hieracium scheppigianum flowering aerial parts dichloromethane extract (SCH), and antihyperalgesic and antiedematous activities of previously chemically characterised polyphenol-rich H. glabratum and H. calophyllum flowering aerial parts methanol extracts (GLA and CAL, respectively). α- and β-Amyrin and their acetates, and lupeol acetate were identified and quantified in SCH by GC-FID and GC-MS. In carrageenan-induced localised inflammation model in rats, SCH and GLA (50–200 mg/kg, p.o.) produced significant and dose-dependent antihyperalgesic effect of 26.9%–56.2% (ED50=163.0 ± 26.5 mg/kg) and 25.3%–51.6% (ED50=211.6 ± 70.6 mg/kg), respectively, and CAL (200 mg/kg, p.o.) exhibited effect of 38.1%. Extracts did not significantly reduce paw edema. SCH and GLA, which demonstrated higher (over 50%) antihyperalgesic efficacy, were tested in a rotarod test (200 mg/kg, p.o.) and no alteration of motor coordination was observed. Also, acute administration of SCH and GLA in mice (2000 mg/kg, p.o.) caused neither mortality nor toxicity.
PB  - Taylor & Francis Ltd
T2  - Natural Product Research
T1  - Investigation of antihyperalgesic and antiedematous activities of three Hieracium species
VL  - 35
IS  - 23
SP  - 5384
EP  - 5388
DO  - 10.1080/14786419.2020.1768086
ER  - 

@article{
author = "Milutinović, Violeta and Pecikoza, Uroš and Tomić, Maja and Stepanović-Petrović, Radica and Niketić, Marjan and Ušjak, Ljuboš and Petrović, Silvana",
year = "2021",
abstract = "Present study investigated triterpene profile, antihyperalgesic and antiedematous activities of Hieracium scheppigianum flowering aerial parts dichloromethane extract (SCH), and antihyperalgesic and antiedematous activities of previously chemically characterised polyphenol-rich H. glabratum and H. calophyllum flowering aerial parts methanol extracts (GLA and CAL, respectively). α- and β-Amyrin and their acetates, and lupeol acetate were identified and quantified in SCH by GC-FID and GC-MS. In carrageenan-induced localised inflammation model in rats, SCH and GLA (50–200 mg/kg, p.o.) produced significant and dose-dependent antihyperalgesic effect of 26.9%–56.2% (ED50=163.0 ± 26.5 mg/kg) and 25.3%–51.6% (ED50=211.6 ± 70.6 mg/kg), respectively, and CAL (200 mg/kg, p.o.) exhibited effect of 38.1%. Extracts did not significantly reduce paw edema. SCH and GLA, which demonstrated higher (over 50%) antihyperalgesic efficacy, were tested in a rotarod test (200 mg/kg, p.o.) and no alteration of motor coordination was observed. Also, acute administration of SCH and GLA in mice (2000 mg/kg, p.o.) caused neither mortality nor toxicity.",
publisher = "Taylor & Francis Ltd",
journal = "Natural Product Research",
title = "Investigation of antihyperalgesic and antiedematous activities of three Hieracium species",
volume = "35",
number = "23",
pages = "5384-5388",
doi = "10.1080/14786419.2020.1768086"
}

Milutinović, V., Pecikoza, U., Tomić, M., Stepanović-Petrović, R., Niketić, M., Ušjak, L.,& Petrović, S.. (2021). Investigation of antihyperalgesic and antiedematous activities of three Hieracium species. in Natural Product Research
Taylor & Francis Ltd., 35(23), 5384-5388.
https://doi.org/10.1080/14786419.2020.1768086

Milutinović V, Pecikoza U, Tomić M, Stepanović-Petrović R, Niketić M, Ušjak L, Petrović S. Investigation of antihyperalgesic and antiedematous activities of three Hieracium species. in Natural Product Research. 2021;35(23):5384-5388.
doi:10.1080/14786419.2020.1768086 .

Milutinović, Violeta, Pecikoza, Uroš, Tomić, Maja, Stepanović-Petrović, Radica, Niketić, Marjan, Ušjak, Ljuboš, Petrović, Silvana, "Investigation of antihyperalgesic and antiedematous activities of three Hieracium species" in Natural Product Research, 35, no. 23 (2021):5384-5388,
https://doi.org/10.1080/14786419.2020.1768086 . .

TY  - JOUR
AU  - Ilić, Milan D.
AU  - Marčetić, Mirjana
AU  - Zlatković, Bojan K.
AU  - Lakušić, Branislava
AU  - Kovačević, Nada
AU  - Drobac, Milica
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3533
AB  - Geranium species are widely used in traditional medicine of Balkan. The aim of this work was to investigate and compare chemical composition of volatile fractions obtained by hydrodistillation from aerial parts of G. macrorrhizum, G. phaeum, G. sanguineum, G. robertianum, G. palustre, G. pyrenaicum, G. columbinum and G. lucidum as well as from underground parts of G. macrorrhizum and G. phaeum, originated from Vlasina plateau in South Eastern Serbia. The volatiles were analyzed using GC/MS and GC-FID. G. palustre volatiles have been studied for the first time with β-selinene (18.6 %) as a characteristic compound. The cluster analysis revealed separation of volatiles into two main groups. Volatile fractions of G. macrorrhizum were separated from all other samples due to high sesquiterpene content (92.3 % in aerial and 94.6 % in underground parts). The volatile fractions of other samples were mainly composed of sesquiterpenes (10.8–61.8 %), diterpenes (12.9–43.0 %) and fatty acids and their derivatives (6.6–21.6 %) with the exception of volatile fraction of G. phaeum underground parts which was dominated only by fatty acids and their derivatives (76.6 %). The results presented in this article contribute to the knowledge on the chemistry of this genus and advances the knowledge on flora of southeast Serbia.
PB  - Wiley-VHCA AG
T2  - Chemistry and Biodiversity
T1  - Chemical Composition of Volatiles of Eight Geranium L. Species from Vlasina Plateau (South Eastern Serbia)
VL  - 17
IS  - 2
DO  - 10.1002/cbdv.201900544
ER  - 

@article{
author = "Ilić, Milan D. and Marčetić, Mirjana and Zlatković, Bojan K. and Lakušić, Branislava and Kovačević, Nada and Drobac, Milica",
year = "2020",
abstract = "Geranium species are widely used in traditional medicine of Balkan. The aim of this work was to investigate and compare chemical composition of volatile fractions obtained by hydrodistillation from aerial parts of G. macrorrhizum, G. phaeum, G. sanguineum, G. robertianum, G. palustre, G. pyrenaicum, G. columbinum and G. lucidum as well as from underground parts of G. macrorrhizum and G. phaeum, originated from Vlasina plateau in South Eastern Serbia. The volatiles were analyzed using GC/MS and GC-FID. G. palustre volatiles have been studied for the first time with β-selinene (18.6 %) as a characteristic compound. The cluster analysis revealed separation of volatiles into two main groups. Volatile fractions of G. macrorrhizum were separated from all other samples due to high sesquiterpene content (92.3 % in aerial and 94.6 % in underground parts). The volatile fractions of other samples were mainly composed of sesquiterpenes (10.8–61.8 %), diterpenes (12.9–43.0 %) and fatty acids and their derivatives (6.6–21.6 %) with the exception of volatile fraction of G. phaeum underground parts which was dominated only by fatty acids and their derivatives (76.6 %). The results presented in this article contribute to the knowledge on the chemistry of this genus and advances the knowledge on flora of southeast Serbia.",
publisher = "Wiley-VHCA AG",
journal = "Chemistry and Biodiversity",
title = "Chemical Composition of Volatiles of Eight Geranium L. Species from Vlasina Plateau (South Eastern Serbia)",
volume = "17",
number = "2",
doi = "10.1002/cbdv.201900544"
}

Ilić, M. D., Marčetić, M., Zlatković, B. K., Lakušić, B., Kovačević, N.,& Drobac, M.. (2020). Chemical Composition of Volatiles of Eight Geranium L. Species from Vlasina Plateau (South Eastern Serbia). in Chemistry and Biodiversity
Wiley-VHCA AG., 17(2).
https://doi.org/10.1002/cbdv.201900544

Ilić MD, Marčetić M, Zlatković BK, Lakušić B, Kovačević N, Drobac M. Chemical Composition of Volatiles of Eight Geranium L. Species from Vlasina Plateau (South Eastern Serbia). in Chemistry and Biodiversity. 2020;17(2).
doi:10.1002/cbdv.201900544 .

Ilić, Milan D., Marčetić, Mirjana, Zlatković, Bojan K., Lakušić, Branislava, Kovačević, Nada, Drobac, Milica, "Chemical Composition of Volatiles of Eight Geranium L. Species from Vlasina Plateau (South Eastern Serbia)" in Chemistry and Biodiversity, 17, no. 2 (2020),
https://doi.org/10.1002/cbdv.201900544 . .

TY  - JOUR
AU  - Rajković, Katarina M
AU  - Vasić, Marijana
AU  - Drobac, Milica
AU  - Mutić, Jelena
AU  - Jeremić, Sanja
AU  - Simić, Valentina
AU  - Stanković, Jovan
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3604
AB  - The extraction yield of Juglans nigra L. leaves was assessed at different ethanol concen-trations (0–96% (v/v)) and solvent-to-solid ratios (5–20 kg kg−1). The response surfacemethodology (RSM) and artificial neural network with genetic algorithms (ANN-GA) weredeveloped to optimize the extraction variables. The RSM and ANN-GA models determined50% (v/v) ethanol concentration and 20 kg kg−1solvent-to-solid ratio as optimal conditions,ensuring an extraction yield of 27.69 and 27.19 g 100 g−1of dry leaves. The phenolic com-pounds in optimal extract were quantified: 3-O-caffeoylquinic acid (2.27 mg g−1of dry leaves),quercetin-3-O-galactoside (10.99 mg g−1of dry leaves) and quercetin-3-O-rhamnoside (15.07mg g−1of dry leaves) using high-performance liquid chromatography (HPLC). The mineralsin optimal extract were quantified: macro-elements (the relative order by content was: K> Mg > Ca) using inductively coupled plasma optical emission spectrometry (ICP-OES) andmicro-elements (the relative order by content was: Zn > Rb > Mn > I>Sr > Ni > Cu > Co >V > Ag > Se) using inductively coupled plasma mass spectrometry (ICP-MS). The extrac-tion coefficients for minerals were determined and were highest for K (64.3%) and I (53.5%).Optimization of extraction process resulted in high extraction yield from J. nigra leaves andoptimal extract containing different phytochemical compounds.
PB  - Elsevier
T2  - Chemical Engineering Research and Design
T1  - Optimization of extraction yield and chemical characterization of optimal extract from Juglans nigra L. leaves
VL  - 157
SP  - 25
EP  - 33
DO  - doi.org/10.1016/j.cherd.2020.03.002
ER  - 

@article{
author = "Rajković, Katarina M and Vasić, Marijana and Drobac, Milica and Mutić, Jelena and Jeremić, Sanja and Simić, Valentina and Stanković, Jovan",
year = "2020",
abstract = "The extraction yield of Juglans nigra L. leaves was assessed at different ethanol concen-trations (0–96% (v/v)) and solvent-to-solid ratios (5–20 kg kg−1). The response surfacemethodology (RSM) and artificial neural network with genetic algorithms (ANN-GA) weredeveloped to optimize the extraction variables. The RSM and ANN-GA models determined50% (v/v) ethanol concentration and 20 kg kg−1solvent-to-solid ratio as optimal conditions,ensuring an extraction yield of 27.69 and 27.19 g 100 g−1of dry leaves. The phenolic com-pounds in optimal extract were quantified: 3-O-caffeoylquinic acid (2.27 mg g−1of dry leaves),quercetin-3-O-galactoside (10.99 mg g−1of dry leaves) and quercetin-3-O-rhamnoside (15.07mg g−1of dry leaves) using high-performance liquid chromatography (HPLC). The mineralsin optimal extract were quantified: macro-elements (the relative order by content was: K> Mg > Ca) using inductively coupled plasma optical emission spectrometry (ICP-OES) andmicro-elements (the relative order by content was: Zn > Rb > Mn > I>Sr > Ni > Cu > Co >V > Ag > Se) using inductively coupled plasma mass spectrometry (ICP-MS). The extrac-tion coefficients for minerals were determined and were highest for K (64.3%) and I (53.5%).Optimization of extraction process resulted in high extraction yield from J. nigra leaves andoptimal extract containing different phytochemical compounds.",
publisher = "Elsevier",
journal = "Chemical Engineering Research and Design",
title = "Optimization of extraction yield and chemical characterization of optimal extract from Juglans nigra L. leaves",
volume = "157",
pages = "25-33",
doi = "doi.org/10.1016/j.cherd.2020.03.002"
}

Rajković, K. M., Vasić, M., Drobac, M., Mutić, J., Jeremić, S., Simić, V.,& Stanković, J.. (2020). Optimization of extraction yield and chemical characterization of optimal extract from Juglans nigra L. leaves. in Chemical Engineering Research and Design
Elsevier., 157, 25-33.
https://doi.org/doi.org/10.1016/j.cherd.2020.03.002

Rajković KM, Vasić M, Drobac M, Mutić J, Jeremić S, Simić V, Stanković J. Optimization of extraction yield and chemical characterization of optimal extract from Juglans nigra L. leaves. in Chemical Engineering Research and Design. 2020;157:25-33.
doi:doi.org/10.1016/j.cherd.2020.03.002 .

Rajković, Katarina M, Vasić, Marijana, Drobac, Milica, Mutić, Jelena, Jeremić, Sanja, Simić, Valentina, Stanković, Jovan, "Optimization of extraction yield and chemical characterization of optimal extract from Juglans nigra L. leaves" in Chemical Engineering Research and Design, 157 (2020):25-33,
https://doi.org/doi.org/10.1016/j.cherd.2020.03.002 . .

TY  - JOUR
AU  - Ušjak, Ljuboš
AU  - Niketić, Marjan
AU  - Drobac, Milica
AU  - Petrović, Silvana
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3530
AB  - The composition of leaf and flower essential oils of eight Heracleum taxa (populations collected in Serbia, Montenegro, North Macedonia and Slovenia) was statistically analyzed to evaluate its chemosystematic significance. Investigated taxa included H. orphanidis and the representatives of H. sphondylium group: H. sphondylium, H. sibiricum, H. montanum, H. ternatum, H. pyrenaicum subsp. pollinianum, H. pyrenaicum subsp. orsinii and H. verticillatum. Hydrodistilled essential oils were analyzed by GC–FID and GC–MS. Chemosystematic significance was evaluated using multivariate statistics: principal component analysis (PCA), non-metric multidimensional scaling (NMDS) and unweighted pair-group arithmetic averages clustering (UPGMA). Statistical analyses included our previously published data on the composition of essential oils of eight leaf and three flower samples, as well as the data on the composition of the oils of additional eight leaf and five flower samples obtained in the current work. Leaf and flower essential oils of H. sphondylium group members were dominated by various sesquiterpenes, phenylpropanoids and/or monoterpenes. Heracleum orphanidis leaf and flower essential oils were rich in aliphatic esters, mostly octyl acetate. Statistical analysis of the composition of leaf essential oils, as well as of flower oils, demonstrated the grouping of investigated populations of these Heracleum taxa according to their systematics, i.e., separation of H. orphanidis from the representatives of H. sphondylium group, and grouping of H. pyrenaicum subspecies within this group. Morphologically related H. sibiricum and H. ternatum were closely located in PCA and NMDS and in UPGMA even shared the same cluster.
PB  - Springer Nature
T2  - Plant Systematics and Evolution
T1  - Chemosystematic evaluation of leaf and flower essential oils of eight Heracleum taxa from Southeastern Europe
VL  - 306
IS  - 1
DO  - 10.1007/s00606-020-01645-z
ER  - 

@article{
author = "Ušjak, Ljuboš and Niketić, Marjan and Drobac, Milica and Petrović, Silvana",
year = "2020",
abstract = "The composition of leaf and flower essential oils of eight Heracleum taxa (populations collected in Serbia, Montenegro, North Macedonia and Slovenia) was statistically analyzed to evaluate its chemosystematic significance. Investigated taxa included H. orphanidis and the representatives of H. sphondylium group: H. sphondylium, H. sibiricum, H. montanum, H. ternatum, H. pyrenaicum subsp. pollinianum, H. pyrenaicum subsp. orsinii and H. verticillatum. Hydrodistilled essential oils were analyzed by GC–FID and GC–MS. Chemosystematic significance was evaluated using multivariate statistics: principal component analysis (PCA), non-metric multidimensional scaling (NMDS) and unweighted pair-group arithmetic averages clustering (UPGMA). Statistical analyses included our previously published data on the composition of essential oils of eight leaf and three flower samples, as well as the data on the composition of the oils of additional eight leaf and five flower samples obtained in the current work. Leaf and flower essential oils of H. sphondylium group members were dominated by various sesquiterpenes, phenylpropanoids and/or monoterpenes. Heracleum orphanidis leaf and flower essential oils were rich in aliphatic esters, mostly octyl acetate. Statistical analysis of the composition of leaf essential oils, as well as of flower oils, demonstrated the grouping of investigated populations of these Heracleum taxa according to their systematics, i.e., separation of H. orphanidis from the representatives of H. sphondylium group, and grouping of H. pyrenaicum subspecies within this group. Morphologically related H. sibiricum and H. ternatum were closely located in PCA and NMDS and in UPGMA even shared the same cluster.",
publisher = "Springer Nature",
journal = "Plant Systematics and Evolution",
title = "Chemosystematic evaluation of leaf and flower essential oils of eight Heracleum taxa from Southeastern Europe",
volume = "306",
number = "1",
doi = "10.1007/s00606-020-01645-z"
}

Ušjak, L., Niketić, M., Drobac, M.,& Petrović, S.. (2020). Chemosystematic evaluation of leaf and flower essential oils of eight Heracleum taxa from Southeastern Europe. in Plant Systematics and Evolution
Springer Nature., 306(1).
https://doi.org/10.1007/s00606-020-01645-z

Ušjak L, Niketić M, Drobac M, Petrović S. Chemosystematic evaluation of leaf and flower essential oils of eight Heracleum taxa from Southeastern Europe. in Plant Systematics and Evolution. 2020;306(1).
doi:10.1007/s00606-020-01645-z .

Ušjak, Ljuboš, Niketić, Marjan, Drobac, Milica, Petrović, Silvana, "Chemosystematic evaluation of leaf and flower essential oils of eight Heracleum taxa from Southeastern Europe" in Plant Systematics and Evolution, 306, no. 1 (2020),
https://doi.org/10.1007/s00606-020-01645-z . .

TY  - JOUR
AU  - Ćebović, Tatjana
AU  - Jakovljević, Dunja
AU  - Maksimović, Zoran
AU  - Đorđević, Snežana
AU  - Jakovljević, Sanja
AU  - Četojević-Simin, Dragana
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3592
AB  - Uvod/Cilj: Rumex crispus (štavelj) je višegodišnja zeljasta biljka, koja se smatra bogatim izvorom biološki aktivnih molekula sa antioksidantnom i citotoksičnom aktivnošću. Cilj rada bio je procena antioksidantnog kapaciteta i ispitivanje antitumorske aktivnosti vodenog ekstrakta ploda štavelja. Metode. Antioksidantna aktivnost vodenog ekstrakta ploda štavelja procenjena je na osnovu in vitro testova: ferric-reducing antioxidant power (FRAP), sposobnosti ekstrakta da neutrališe slobodne radikale NO•, OH• i 2,2-difentil-1-pikril hidrazil (DPPH) i utiče na lipidnu peroksidaciju u lipozomima. Citotoksičnost ispitivanog ekstrakta je određena in vitro na tumorskim ćelijskim linijama: humani karcinom cerviksa (HeLa), adenokarcinom (HT-29) i adenokarcinom dojke (MCF7). Rezultati. Testirani ekstrakt pokazao je potencijalnu antioksidantnu aktivnost manifestovanu velikom moći u neutralizaciji slobodnih radikala, kao i sposobnost da smanji lipidnu peroksidaciju u lipozomima. Ustanovljena je tkivno-selektivnu citotoksičnost ekstrakta ploda štavelja in vitro. Najizraženija antitumorska aktivnost primećena je prema HeLa i MCF7 ćelijskim linijama. Zaključak. Vodeni ekstrakt ploda štavelja ima potencijalnu antioksidantnu i citotoksičnu aktivnost, sa nekrozom kao glavnim mehanizmom indukovane ćelijske smrti. Za dalja istraživanja, preporučuju se i druge metode ekstrakcije ploda štavelja.
AB  - Background/Aim. Rumex crispus (curly dock) is a wild perennial herbaceous plant, which products are considered as a rich source of biologically active molecules with antioxidant and cytotoxic activities. The aim of this study was to estimate of antioxidant and cytotoxic activities of aqueous extract of curly dock fruits. Methods. The aqueous extract of curly dock fruits was evaluated for its antioxidant activity by in vitro assays for ferric-reducing antioxidant power (FRAP), NO•, OH• and 2,2-diphenyl-1-picrylhydrazyl (DPPH)-free radical scavenging activities and the influence on lipid peroxidation in liposomes. The cytotoxicity of tested extract was examined in vitro in human cervix carcinoma (HeLa), colon adenocarcinoma (HT-29) and breast adenocarcinoma (MCF7) cells. Results. The tested extract showed a potential antioxidant activity manifested in scavenging of free radicals as well as an ability to decrease lipid peroxidation in liposomes. The results indicated tissue-selective cytotoxicity of R. crispus fruit extract in vitro. The most prominent antitumor activity was observed towards HeLa and MCF7 cell lines. Conclusion. The investigated aqueous fruit extract of R. crispus had potential antioxidant and cytotoxic activities, with necrosis as a main mechanism of induced cell-death. Different methods of extraction of R. crispus fruits, apart from aqueous, are recommended for further investigations.
PB  - Beograd : Vojnomedicinska akademija - Institut za naučne informacije
T2  - Vojnosanitetski pregled
T1  - Antioxidant and cytotoxic activities of curly dock (Rumex crispus L., Polygonaceae) fruit extract
T1  - Antioksidantna i citotoksična aktivnost ekstrakta ploda štavelja (Rumex crispus L., Polygonaceae)
VL  - 77
IS  - 3
SP  - 308
EP  - 316
DO  - 10.2298/VSP170713084C
ER  - 

@article{
author = "Ćebović, Tatjana and Jakovljević, Dunja and Maksimović, Zoran and Đorđević, Snežana and Jakovljević, Sanja and Četojević-Simin, Dragana",
year = "2020",
abstract = "Uvod/Cilj: Rumex crispus (štavelj) je višegodišnja zeljasta biljka, koja se smatra bogatim izvorom biološki aktivnih molekula sa antioksidantnom i citotoksičnom aktivnošću. Cilj rada bio je procena antioksidantnog kapaciteta i ispitivanje antitumorske aktivnosti vodenog ekstrakta ploda štavelja. Metode. Antioksidantna aktivnost vodenog ekstrakta ploda štavelja procenjena je na osnovu in vitro testova: ferric-reducing antioxidant power (FRAP), sposobnosti ekstrakta da neutrališe slobodne radikale NO•, OH• i 2,2-difentil-1-pikril hidrazil (DPPH) i utiče na lipidnu peroksidaciju u lipozomima. Citotoksičnost ispitivanog ekstrakta je određena in vitro na tumorskim ćelijskim linijama: humani karcinom cerviksa (HeLa), adenokarcinom (HT-29) i adenokarcinom dojke (MCF7). Rezultati. Testirani ekstrakt pokazao je potencijalnu antioksidantnu aktivnost manifestovanu velikom moći u neutralizaciji slobodnih radikala, kao i sposobnost da smanji lipidnu peroksidaciju u lipozomima. Ustanovljena je tkivno-selektivnu citotoksičnost ekstrakta ploda štavelja in vitro. Najizraženija antitumorska aktivnost primećena je prema HeLa i MCF7 ćelijskim linijama. Zaključak. Vodeni ekstrakt ploda štavelja ima potencijalnu antioksidantnu i citotoksičnu aktivnost, sa nekrozom kao glavnim mehanizmom indukovane ćelijske smrti. Za dalja istraživanja, preporučuju se i druge metode ekstrakcije ploda štavelja., Background/Aim. Rumex crispus (curly dock) is a wild perennial herbaceous plant, which products are considered as a rich source of biologically active molecules with antioxidant and cytotoxic activities. The aim of this study was to estimate of antioxidant and cytotoxic activities of aqueous extract of curly dock fruits. Methods. The aqueous extract of curly dock fruits was evaluated for its antioxidant activity by in vitro assays for ferric-reducing antioxidant power (FRAP), NO•, OH• and 2,2-diphenyl-1-picrylhydrazyl (DPPH)-free radical scavenging activities and the influence on lipid peroxidation in liposomes. The cytotoxicity of tested extract was examined in vitro in human cervix carcinoma (HeLa), colon adenocarcinoma (HT-29) and breast adenocarcinoma (MCF7) cells. Results. The tested extract showed a potential antioxidant activity manifested in scavenging of free radicals as well as an ability to decrease lipid peroxidation in liposomes. The results indicated tissue-selective cytotoxicity of R. crispus fruit extract in vitro. The most prominent antitumor activity was observed towards HeLa and MCF7 cell lines. Conclusion. The investigated aqueous fruit extract of R. crispus had potential antioxidant and cytotoxic activities, with necrosis as a main mechanism of induced cell-death. Different methods of extraction of R. crispus fruits, apart from aqueous, are recommended for further investigations.",
publisher = "Beograd : Vojnomedicinska akademija - Institut za naučne informacije",
journal = "Vojnosanitetski pregled",
title = "Antioxidant and cytotoxic activities of curly dock (Rumex crispus L., Polygonaceae) fruit extract, Antioksidantna i citotoksična aktivnost ekstrakta ploda štavelja (Rumex crispus L., Polygonaceae)",
volume = "77",
number = "3",
pages = "308-316",
doi = "10.2298/VSP170713084C"
}

Ćebović, T., Jakovljević, D., Maksimović, Z., Đorđević, S., Jakovljević, S.,& Četojević-Simin, D.. (2020). Antioxidant and cytotoxic activities of curly dock (Rumex crispus L., Polygonaceae) fruit extract. in Vojnosanitetski pregled
Beograd : Vojnomedicinska akademija - Institut za naučne informacije., 77(3), 308-316.
https://doi.org/10.2298/VSP170713084C

Ćebović T, Jakovljević D, Maksimović Z, Đorđević S, Jakovljević S, Četojević-Simin D. Antioxidant and cytotoxic activities of curly dock (Rumex crispus L., Polygonaceae) fruit extract. in Vojnosanitetski pregled. 2020;77(3):308-316.
doi:10.2298/VSP170713084C .

Ćebović, Tatjana, Jakovljević, Dunja, Maksimović, Zoran, Đorđević, Snežana, Jakovljević, Sanja, Četojević-Simin, Dragana, "Antioxidant and cytotoxic activities of curly dock (Rumex crispus L., Polygonaceae) fruit extract" in Vojnosanitetski pregled, 77, no. 3 (2020):308-316,
https://doi.org/10.2298/VSP170713084C . .

TY  - CONF
AU  - Zbiljić, Miloš
AU  - Lakušić, Branislava
AU  - Marčetić, Mirjana
AU  - Bogdanović, Sandro
AU  - Lakušić, Dmitar
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5304
AB  - Teucrium × rohlenae Maly is a natural hybrid between T. montanum and T.
polium. It was found in the surrounding of Kotor (Montenegro) by L. Čelakovski fil.
and reported by J. Rohlena as T montanum × polium in 1922. This hybrid is validly
described by K. Maly in 1951. After the original description, Teucrium × rohlenae
have not been reported in the Balkan Peninsula, but hybrids between T montanum and
T. polium have been cited in Italy (as Teucrium x castrense) and Moldova (as
Teucrium × bogoutdinovae). In sympatric population of T. montanum and T. polium
in the surrounding of Trilj near Split (Croatia), we found intermediate individuals with
potentially hybridogenic origin. The aim of the study was to analyse 44 morphoanatomical
characters of stem, leaf, inflorescens and calyx, and chemical composition
of volatile compounds of parental species and their potential hybrid Teucrium ×
rohlenae. The composition of hexane extracts of aerial parts was determined using
GC-FID and GC-MS methods. A detailed morpho-anatomical analysis has shown that
parental species are well differentiated, while potential hybrids have an intermediate
position. Volatiles of potential hybrid represented both species, main hydrocarbon
compounds were alike volatiles of T. polium while the terpene profile was more
similar to T. montanum terpenes.
PB  - Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš
PB  - Institute for Nature Conservation of Serbia
C3  - 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts
T1  - Teucrium × rohlenae, a new natural hybrid in flora of Croatia - Morphological and chemical evidence
SP  - 16
EP  - 16
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5304
ER  - 

@conference{
author = "Zbiljić, Miloš and Lakušić, Branislava and Marčetić, Mirjana and Bogdanović, Sandro and Lakušić, Dmitar",
year = "2019",
abstract = "Teucrium × rohlenae Maly is a natural hybrid between T. montanum and T.
polium. It was found in the surrounding of Kotor (Montenegro) by L. Čelakovski fil.
and reported by J. Rohlena as T montanum × polium in 1922. This hybrid is validly
described by K. Maly in 1951. After the original description, Teucrium × rohlenae
have not been reported in the Balkan Peninsula, but hybrids between T montanum and
T. polium have been cited in Italy (as Teucrium x castrense) and Moldova (as
Teucrium × bogoutdinovae). In sympatric population of T. montanum and T. polium
in the surrounding of Trilj near Split (Croatia), we found intermediate individuals with
potentially hybridogenic origin. The aim of the study was to analyse 44 morphoanatomical
characters of stem, leaf, inflorescens and calyx, and chemical composition
of volatile compounds of parental species and their potential hybrid Teucrium ×
rohlenae. The composition of hexane extracts of aerial parts was determined using
GC-FID and GC-MS methods. A detailed morpho-anatomical analysis has shown that
parental species are well differentiated, while potential hybrids have an intermediate
position. Volatiles of potential hybrid represented both species, main hydrocarbon
compounds were alike volatiles of T. polium while the terpene profile was more
similar to T. montanum terpenes.",
publisher = "Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, Institute for Nature Conservation of Serbia",
journal = "13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts",
title = "Teucrium × rohlenae, a new natural hybrid in flora of Croatia - Morphological and chemical evidence",
pages = "16-16",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5304"
}

Zbiljić, M., Lakušić, B., Marčetić, M., Bogdanović, S.,& Lakušić, D.. (2019). Teucrium × rohlenae, a new natural hybrid in flora of Croatia - Morphological and chemical evidence. in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts
Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš., 16-16.
https://hdl.handle.net/21.15107/rcub_farfar_5304

Zbiljić M, Lakušić B, Marčetić M, Bogdanović S, Lakušić D. Teucrium × rohlenae, a new natural hybrid in flora of Croatia - Morphological and chemical evidence. in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts. 2019;:16-16.
https://hdl.handle.net/21.15107/rcub_farfar_5304 .

Zbiljić, Miloš, Lakušić, Branislava, Marčetić, Mirjana, Bogdanović, Sandro, Lakušić, Dmitar, "Teucrium × rohlenae, a new natural hybrid in flora of Croatia - Morphological and chemical evidence" in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts (2019):16-16,
https://hdl.handle.net/21.15107/rcub_farfar_5304 .

TY  - CONF
AU  - Marčetić, Mirjana
AU  - Obradović, Marijana
AU  - Tomić, Emilija
AU  - Teovanović, Anđelka
AU  - Lakušić, Branislava
AU  - Božić, Dragana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5303
AB  - Lavender flower and essential oil are traditionally used for relief of mild
mental stress and to aid sleep. Staphylococcus spp. are part of skin microbiota and
frequent contaminants of the wounds. The aim of the study was to investigate the
composition and antimicrobial activity of lavender oils on standard strains of bacteria,
wound isolates of Staphylococcus epidermidis and S. aureus and also the activity of
the oil in the combination with gentamicin. The essential oils were analysed by GCFID/
GC-MS. Sensitivity of seven standard bacterial strains and eight wound isolates
of staphylococci were examined by disk-diffusion or broth-microdilution method. The
dominant components in the essential oils of lavender flower were: linalool, linalyl
acetate and camphor and in oil of leaves and stems borneol, 1,8-cineol and camphor.
The essential oil of lavender inhibited the growth of laboratory bacterial strains (MIC
0.125 – 1 mg/ml). The best activity (0.125 mg/ml) was obtained with commercial
samples that contained higher content of camphor and borneol. The essential oil of
lavender flower (2%) inhibited the growth of multiresistant wound isolates of
staphylococci. Significant synergistic effects against Staphylococcus spp. were
observed in combinations of subinhibitory concentrations of essential oils (15.6 – 31.2
μg/ml) and gentamicine (0.06 – 0.25 μg/ml).
PB  - Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš
PB  - Institute for Nature Conservation of Serbia
C3  - 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts
T1  - Composition and antimicrobial activity of lavender, Lavandula angustifolia Mill. (Lamiaceae) essential oil against standard strains and wound isolates of bacteria
SP  - 151
EP  - 151
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5303
ER  - 

@conference{
author = "Marčetić, Mirjana and Obradović, Marijana and Tomić, Emilija and Teovanović, Anđelka and Lakušić, Branislava and Božić, Dragana",
year = "2019",
abstract = "Lavender flower and essential oil are traditionally used for relief of mild
mental stress and to aid sleep. Staphylococcus spp. are part of skin microbiota and
frequent contaminants of the wounds. The aim of the study was to investigate the
composition and antimicrobial activity of lavender oils on standard strains of bacteria,
wound isolates of Staphylococcus epidermidis and S. aureus and also the activity of
the oil in the combination with gentamicin. The essential oils were analysed by GCFID/
GC-MS. Sensitivity of seven standard bacterial strains and eight wound isolates
of staphylococci were examined by disk-diffusion or broth-microdilution method. The
dominant components in the essential oils of lavender flower were: linalool, linalyl
acetate and camphor and in oil of leaves and stems borneol, 1,8-cineol and camphor.
The essential oil of lavender inhibited the growth of laboratory bacterial strains (MIC
0.125 – 1 mg/ml). The best activity (0.125 mg/ml) was obtained with commercial
samples that contained higher content of camphor and borneol. The essential oil of
lavender flower (2%) inhibited the growth of multiresistant wound isolates of
staphylococci. Significant synergistic effects against Staphylococcus spp. were
observed in combinations of subinhibitory concentrations of essential oils (15.6 – 31.2
μg/ml) and gentamicine (0.06 – 0.25 μg/ml).",
publisher = "Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, Institute for Nature Conservation of Serbia",
journal = "13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts",
title = "Composition and antimicrobial activity of lavender, Lavandula angustifolia Mill. (Lamiaceae) essential oil against standard strains and wound isolates of bacteria",
pages = "151-151",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5303"
}

Marčetić, M., Obradović, M., Tomić, E., Teovanović, A., Lakušić, B.,& Božić, D.. (2019). Composition and antimicrobial activity of lavender, Lavandula angustifolia Mill. (Lamiaceae) essential oil against standard strains and wound isolates of bacteria. in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts
Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš., 151-151.
https://hdl.handle.net/21.15107/rcub_farfar_5303

Marčetić M, Obradović M, Tomić E, Teovanović A, Lakušić B, Božić D. Composition and antimicrobial activity of lavender, Lavandula angustifolia Mill. (Lamiaceae) essential oil against standard strains and wound isolates of bacteria. in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts. 2019;:151-151.
https://hdl.handle.net/21.15107/rcub_farfar_5303 .

Marčetić, Mirjana, Obradović, Marijana, Tomić, Emilija, Teovanović, Anđelka, Lakušić, Branislava, Božić, Dragana, "Composition and antimicrobial activity of lavender, Lavandula angustifolia Mill. (Lamiaceae) essential oil against standard strains and wound isolates of bacteria" in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts (2019):151-151,
https://hdl.handle.net/21.15107/rcub_farfar_5303 .

TY  - CONF
AU  - Slavkovska, Violeta
AU  - Zlatković, Bojan
AU  - Lakušić, Branislava
AU  - Kovačević, Nada
AU  - Drobac, Milica
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5293
AB  - This study explores the shoot anatomical structure, content and composition of
the essential oils of the vegetative organs and fruit of the Pimpinella alpina. The plant
material was collected at Mt. Besna Kobila in South-Eastern Serbia, and Šar planina
in Metohija. The anatomical studies were conducted on permanent slides obtained by
standard method of preparation. The essential oils obtained by hydrodistillation from
flowering aerial parts, fruits and roots (from flowering and fruiting period), were
analysed by GC-FID and GC-MS. Anatomical analysis showed that the rhizome has
a secondary structure in the bark zone, while in the zone of vascular tissues it has a
primary structure. The stem has a primary structure, with vascular bundles. The leaf
is dorsiventral, amphistomatic. Secretory channels are numerous in all organs.
Sesquiterpene hydrocarbons with (E)-β-farnesene (23.1-52.1%) were dominant in the
essential oils from aerial parts, fruit and roots, except in the root sample from Šar
planina, which was characterised by high amount of phenylpropanoids (44.2%) with
epoxy-pseudoisoeugenyl 2-methylbutyrate (25.9%). Phenylpropanoids with
pseudoisoeugenol skeleton, which are unique to Pimpinella species essential oils,
were also present in prominent amounts (7.9-26.8%) in other samples. This is the first
report on content and composition of P. alpina essential oil.
PB  - Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš
PB  - Institute for Nature Conservation of Serbia
C3  - 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts
T1  - Study of anatomy and essential oil of Pimpinella alpina Host (Apiaceae) from Serbia
SP  - 27
EP  - 27
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5293
ER  - 

@conference{
author = "Slavkovska, Violeta and Zlatković, Bojan and Lakušić, Branislava and Kovačević, Nada and Drobac, Milica",
year = "2019",
abstract = "This study explores the shoot anatomical structure, content and composition of
the essential oils of the vegetative organs and fruit of the Pimpinella alpina. The plant
material was collected at Mt. Besna Kobila in South-Eastern Serbia, and Šar planina
in Metohija. The anatomical studies were conducted on permanent slides obtained by
standard method of preparation. The essential oils obtained by hydrodistillation from
flowering aerial parts, fruits and roots (from flowering and fruiting period), were
analysed by GC-FID and GC-MS. Anatomical analysis showed that the rhizome has
a secondary structure in the bark zone, while in the zone of vascular tissues it has a
primary structure. The stem has a primary structure, with vascular bundles. The leaf
is dorsiventral, amphistomatic. Secretory channels are numerous in all organs.
Sesquiterpene hydrocarbons with (E)-β-farnesene (23.1-52.1%) were dominant in the
essential oils from aerial parts, fruit and roots, except in the root sample from Šar
planina, which was characterised by high amount of phenylpropanoids (44.2%) with
epoxy-pseudoisoeugenyl 2-methylbutyrate (25.9%). Phenylpropanoids with
pseudoisoeugenol skeleton, which are unique to Pimpinella species essential oils,
were also present in prominent amounts (7.9-26.8%) in other samples. This is the first
report on content and composition of P. alpina essential oil.",
publisher = "Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, Institute for Nature Conservation of Serbia",
journal = "13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts",
title = "Study of anatomy and essential oil of Pimpinella alpina Host (Apiaceae) from Serbia",
pages = "27-27",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5293"
}

Slavkovska, V., Zlatković, B., Lakušić, B., Kovačević, N.,& Drobac, M.. (2019). Study of anatomy and essential oil of Pimpinella alpina Host (Apiaceae) from Serbia. in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts
Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš., 27-27.
https://hdl.handle.net/21.15107/rcub_farfar_5293

Slavkovska V, Zlatković B, Lakušić B, Kovačević N, Drobac M. Study of anatomy and essential oil of Pimpinella alpina Host (Apiaceae) from Serbia. in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts. 2019;:27-27.
https://hdl.handle.net/21.15107/rcub_farfar_5293 .

Slavkovska, Violeta, Zlatković, Bojan, Lakušić, Branislava, Kovačević, Nada, Drobac, Milica, "Study of anatomy and essential oil of Pimpinella alpina Host (Apiaceae) from Serbia" in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts (2019):27-27,
https://hdl.handle.net/21.15107/rcub_farfar_5293 .

TY  - CONF
AU  - Radović, Jelena
AU  - Dodevska, Margarita
AU  - Kopanja, Đurđica
AU  - Kundaković-Vasović, Tatjana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5309
AB  - St. George's mushroom, Calocybe gambosa, is an edible mushroom from the family Lyophyllaceae. It is widespread in Europe. The colour of the mushroom varies from white, yellow to greyish-brown. ...
PB  - Phytochemical Society of Europe
PB  - Faculty of Pharmacy, University of Lisbon, Lisbon, Portugal
C3  - Natural Products in Drug Discovery and Human Health (NatProdDDH) conference Lisbon, Portugal, 2019, Book of abstracts
T1  - Chemical and nutritive composition of St. George’mushroom, Calocybe gambosa (Fr.) Donk
SP  - 212
EP  - 212
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5309
ER  - 

@conference{
author = "Radović, Jelena and Dodevska, Margarita and Kopanja, Đurđica and Kundaković-Vasović, Tatjana",
year = "2019",
abstract = "St. George's mushroom, Calocybe gambosa, is an edible mushroom from the family Lyophyllaceae. It is widespread in Europe. The colour of the mushroom varies from white, yellow to greyish-brown. ...",
publisher = "Phytochemical Society of Europe, Faculty of Pharmacy, University of Lisbon, Lisbon, Portugal",
journal = "Natural Products in Drug Discovery and Human Health (NatProdDDH) conference Lisbon, Portugal, 2019, Book of abstracts",
title = "Chemical and nutritive composition of St. George’mushroom, Calocybe gambosa (Fr.) Donk",
pages = "212-212",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5309"
}

Radović, J., Dodevska, M., Kopanja, Đ.,& Kundaković-Vasović, T.. (2019). Chemical and nutritive composition of St. George’mushroom, Calocybe gambosa (Fr.) Donk. in Natural Products in Drug Discovery and Human Health (NatProdDDH) conference Lisbon, Portugal, 2019, Book of abstracts
Phytochemical Society of Europe., 212-212.
https://hdl.handle.net/21.15107/rcub_farfar_5309

Radović J, Dodevska M, Kopanja Đ, Kundaković-Vasović T. Chemical and nutritive composition of St. George’mushroom, Calocybe gambosa (Fr.) Donk. in Natural Products in Drug Discovery and Human Health (NatProdDDH) conference Lisbon, Portugal, 2019, Book of abstracts. 2019;:212-212.
https://hdl.handle.net/21.15107/rcub_farfar_5309 .

Radović, Jelena, Dodevska, Margarita, Kopanja, Đurđica, Kundaković-Vasović, Tatjana, "Chemical and nutritive composition of St. George’mushroom, Calocybe gambosa (Fr.) Donk" in Natural Products in Drug Discovery and Human Health (NatProdDDH) conference Lisbon, Portugal, 2019, Book of abstracts (2019):212-212,
https://hdl.handle.net/21.15107/rcub_farfar_5309 .

TY  - CONF
AU  - Arsenijević, Jelena
AU  - Radović, Jelena
AU  - Stanojković, Tatjana
AU  - Ranđelović, Vladimir
AU  - Kundaković-Vasović, Tatjana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5308
AB  - Rumex balcanicus Rech.f. (Polygonaceae) is an endemic species that inhabits central parts of Serbia and Kosovo. Recent studies have demonstrated that the isolates of some Rumex species exhibited anti-inflammatory, antioxidant, anticancer, antidiabetic and antimicrobial activities, and aerial parts of some species are used as food. ...
PB  - Phytochemical Society of Europe
PB  - Faculty of Pharmacy, University of Lisbon, Lisbon, Portugal
C3  - Natural Products in Drug Discovery and Human Health (NatProdDDH) conference Lisbon, Portugal, 2019, Book of abstracts
T1  - Chemical analysis and cytotoxicity of Rumex balcanicus Rech. f. (Polygonaceae)
SP  - 169
EP  - 169
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5308
ER  - 

@conference{
author = "Arsenijević, Jelena and Radović, Jelena and Stanojković, Tatjana and Ranđelović, Vladimir and Kundaković-Vasović, Tatjana",
year = "2019",
abstract = "Rumex balcanicus Rech.f. (Polygonaceae) is an endemic species that inhabits central parts of Serbia and Kosovo. Recent studies have demonstrated that the isolates of some Rumex species exhibited anti-inflammatory, antioxidant, anticancer, antidiabetic and antimicrobial activities, and aerial parts of some species are used as food. ...",
publisher = "Phytochemical Society of Europe, Faculty of Pharmacy, University of Lisbon, Lisbon, Portugal",
journal = "Natural Products in Drug Discovery and Human Health (NatProdDDH) conference Lisbon, Portugal, 2019, Book of abstracts",
title = "Chemical analysis and cytotoxicity of Rumex balcanicus Rech. f. (Polygonaceae)",
pages = "169-169",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5308"
}

Arsenijević, J., Radović, J., Stanojković, T., Ranđelović, V.,& Kundaković-Vasović, T.. (2019). Chemical analysis and cytotoxicity of Rumex balcanicus Rech. f. (Polygonaceae). in Natural Products in Drug Discovery and Human Health (NatProdDDH) conference Lisbon, Portugal, 2019, Book of abstracts
Phytochemical Society of Europe., 169-169.
https://hdl.handle.net/21.15107/rcub_farfar_5308

Arsenijević J, Radović J, Stanojković T, Ranđelović V, Kundaković-Vasović T. Chemical analysis and cytotoxicity of Rumex balcanicus Rech. f. (Polygonaceae). in Natural Products in Drug Discovery and Human Health (NatProdDDH) conference Lisbon, Portugal, 2019, Book of abstracts. 2019;:169-169.
https://hdl.handle.net/21.15107/rcub_farfar_5308 .

Arsenijević, Jelena, Radović, Jelena, Stanojković, Tatjana, Ranđelović, Vladimir, Kundaković-Vasović, Tatjana, "Chemical analysis and cytotoxicity of Rumex balcanicus Rech. f. (Polygonaceae)" in Natural Products in Drug Discovery and Human Health (NatProdDDH) conference Lisbon, Portugal, 2019, Book of abstracts (2019):169-169,
https://hdl.handle.net/21.15107/rcub_farfar_5308 .

TY  - CONF
AU  - Radović, Jelena
AU  - Milenković, Marina
AU  - Ranđelović, Vladimir
AU  - Kundaković-Vasović, Tatjana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5295
AB  - Recent studies demonstrated that Rumex species exhibit various
pharmacological activities, such as antiinflamatory, antioxidant, anticancer,
antimicrobial, but there are almost no data on pharmacological activities of Balcan
endemic species Rumex balcanicus. Thus, the aim was to examine antioxidant
activity, using DPPH and FRAP method, antimicrobial activity via broth
microdilution method and α-amylase inhibitory activity using DNS reagent of
aqueous and methanol extracts of roots and aerial parts of R. balcanicus, collected on
Vlasina Lake in 2014. All tested extracts exhibited high antioxidant activity which
has been correlated with the high content of polyphenols. Aqueous extracts exhibited
lower activity compared to methanol extracts. Methanol extract ofR. balcanicus roots
showed the highest activity, both in DPPH (IC50= 9.57 μg/mL) and FRAP test
(IC50=3.98 μmol Fe/mg of extract), while aqueous extract of aerial parts showed the
lowest activity (IC50= 24.73 μg/mL; IC50=2.11 μmol Fe/mg). Only methanol extract
of aerial parts showed α-amylase inhibitory activity with IC50=252 μg/mL. All
extracts had low antimicrobial activity against almost all tested bacterial strains except
against Bacillus subtilis. Positive results obtained in this study show that R. balcanisus
contains potential as a new raw material and for the development of drugs efficient in
treating diabetes.
PB  - Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš
PB  - Institute for Nature Conservation of Serbia
C3  - 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts
T1  - Pharmacological activity of Rumex balcanicus Rech. F. (Polygonaceae)
SP  - 159
EP  - 159
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5295
ER  - 

@conference{
author = "Radović, Jelena and Milenković, Marina and Ranđelović, Vladimir and Kundaković-Vasović, Tatjana",
year = "2019",
abstract = "Recent studies demonstrated that Rumex species exhibit various
pharmacological activities, such as antiinflamatory, antioxidant, anticancer,
antimicrobial, but there are almost no data on pharmacological activities of Balcan
endemic species Rumex balcanicus. Thus, the aim was to examine antioxidant
activity, using DPPH and FRAP method, antimicrobial activity via broth
microdilution method and α-amylase inhibitory activity using DNS reagent of
aqueous and methanol extracts of roots and aerial parts of R. balcanicus, collected on
Vlasina Lake in 2014. All tested extracts exhibited high antioxidant activity which
has been correlated with the high content of polyphenols. Aqueous extracts exhibited
lower activity compared to methanol extracts. Methanol extract ofR. balcanicus roots
showed the highest activity, both in DPPH (IC50= 9.57 μg/mL) and FRAP test
(IC50=3.98 μmol Fe/mg of extract), while aqueous extract of aerial parts showed the
lowest activity (IC50= 24.73 μg/mL; IC50=2.11 μmol Fe/mg). Only methanol extract
of aerial parts showed α-amylase inhibitory activity with IC50=252 μg/mL. All
extracts had low antimicrobial activity against almost all tested bacterial strains except
against Bacillus subtilis. Positive results obtained in this study show that R. balcanisus
contains potential as a new raw material and for the development of drugs efficient in
treating diabetes.",
publisher = "Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, Institute for Nature Conservation of Serbia",
journal = "13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts",
title = "Pharmacological activity of Rumex balcanicus Rech. F. (Polygonaceae)",
pages = "159-159",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5295"
}

Radović, J., Milenković, M., Ranđelović, V.,& Kundaković-Vasović, T.. (2019). Pharmacological activity of Rumex balcanicus Rech. F. (Polygonaceae). in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts
Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš., 159-159.
https://hdl.handle.net/21.15107/rcub_farfar_5295

Radović J, Milenković M, Ranđelović V, Kundaković-Vasović T. Pharmacological activity of Rumex balcanicus Rech. F. (Polygonaceae). in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts. 2019;:159-159.
https://hdl.handle.net/21.15107/rcub_farfar_5295 .

Radović, Jelena, Milenković, Marina, Ranđelović, Vladimir, Kundaković-Vasović, Tatjana, "Pharmacological activity of Rumex balcanicus Rech. F. (Polygonaceae)" in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts (2019):159-159,
https://hdl.handle.net/21.15107/rcub_farfar_5295 .

TY  - CONF
AU  - Arsenijević, Jelena
AU  - Stanojković, Tatjana
AU  - Ranđelović, Vladimir
AU  - Kundaković-Vasović, Tatjana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5294
AB  - Rumex balcanicus Rech.f. (Polygonaceae) is an endemic species that inhabits
central parts of Serbia and Kosovo. The genus Rumex is characterizied by the
accumulation of anthraquinones, naphthalene-1,8-diols, flavonoids and stilbenoids.
Aerial parts of R. balcanicus, collected on Vlasina Lake (Serbia) in 2014, were used
to determine the chemical composition and cytotoxic activity. Cyclohexane,
dichloromethane, methanol and aqueous extracts were prepared and tested using
MTT-test, against human epithelial cervical cancer cells (HeLa), human colon
carcinoma cell line (LS174), human non-small cell lung cancer cells (A549) and
healthy MRC-5 human embryonic lung fibroblast cell line. Methanol and aqueous
extracts were analyzed using LC-MS. In tested extracts, caffeic acid derivates and
flavonol derivatives were identified. The main components in both extracts were rutin,
miquelianin, quercitrin and quercetin 3-O-acetyl-rhamnoside. Caffeic acid and its
glycosides were also present in analyzed extracts. Aqueous extracts had no
cytotoxicity against tested cell lines, while cyclohexane, dichlormethane and
methanol extracts were active against all tested cell lines with IC50 ranged from
116.48±3.26 μg/mL to 178.21±1.30 μg/mL. Due to the presence of flavonoids and
exhibited cytotoxic effect, R. balcanicus herba could be interesting as a plant material
for further pharmacological analysis and isolation of the anti-tumor compounds.
PB  - Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš
PB  - Institute for Nature Conservation of Serbia
C3  - 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts
T1  - Chemical analysis and cytotoxity of Rumex balcanicus Rech.F. (Polygonaceae)
SP  - 158
EP  - 158
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5294
ER  - 

@conference{
author = "Arsenijević, Jelena and Stanojković, Tatjana and Ranđelović, Vladimir and Kundaković-Vasović, Tatjana",
year = "2019",
abstract = "Rumex balcanicus Rech.f. (Polygonaceae) is an endemic species that inhabits
central parts of Serbia and Kosovo. The genus Rumex is characterizied by the
accumulation of anthraquinones, naphthalene-1,8-diols, flavonoids and stilbenoids.
Aerial parts of R. balcanicus, collected on Vlasina Lake (Serbia) in 2014, were used
to determine the chemical composition and cytotoxic activity. Cyclohexane,
dichloromethane, methanol and aqueous extracts were prepared and tested using
MTT-test, against human epithelial cervical cancer cells (HeLa), human colon
carcinoma cell line (LS174), human non-small cell lung cancer cells (A549) and
healthy MRC-5 human embryonic lung fibroblast cell line. Methanol and aqueous
extracts were analyzed using LC-MS. In tested extracts, caffeic acid derivates and
flavonol derivatives were identified. The main components in both extracts were rutin,
miquelianin, quercitrin and quercetin 3-O-acetyl-rhamnoside. Caffeic acid and its
glycosides were also present in analyzed extracts. Aqueous extracts had no
cytotoxicity against tested cell lines, while cyclohexane, dichlormethane and
methanol extracts were active against all tested cell lines with IC50 ranged from
116.48±3.26 μg/mL to 178.21±1.30 μg/mL. Due to the presence of flavonoids and
exhibited cytotoxic effect, R. balcanicus herba could be interesting as a plant material
for further pharmacological analysis and isolation of the anti-tumor compounds.",
publisher = "Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, Institute for Nature Conservation of Serbia",
journal = "13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts",
title = "Chemical analysis and cytotoxity of Rumex balcanicus Rech.F. (Polygonaceae)",
pages = "158-158",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5294"
}

Arsenijević, J., Stanojković, T., Ranđelović, V.,& Kundaković-Vasović, T.. (2019). Chemical analysis and cytotoxity of Rumex balcanicus Rech.F. (Polygonaceae). in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts
Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš., 158-158.
https://hdl.handle.net/21.15107/rcub_farfar_5294

Arsenijević J, Stanojković T, Ranđelović V, Kundaković-Vasović T. Chemical analysis and cytotoxity of Rumex balcanicus Rech.F. (Polygonaceae). in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts. 2019;:158-158.
https://hdl.handle.net/21.15107/rcub_farfar_5294 .

Arsenijević, Jelena, Stanojković, Tatjana, Ranđelović, Vladimir, Kundaković-Vasović, Tatjana, "Chemical analysis and cytotoxity of Rumex balcanicus Rech.F. (Polygonaceae)" in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts (2019):158-158,
https://hdl.handle.net/21.15107/rcub_farfar_5294 .

TY  - JOUR
AU  - Milović, Sanja
AU  - Stanković, Ivan
AU  - Nikolić, Dejan
AU  - Radović, Jelena
AU  - Kolundžić, Marina
AU  - Nikolić, Vesna
AU  - Stanojković, Tatjana
AU  - Petović, Slavica
AU  - Kundaković-Vasović, Tatjana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3468
AB  - Three seaweeds (Halimeda tuna, Codium bursa and Cystoseira barbata) and one seagrass (Cymodocea nodosa) were collected from the Coast of Montenegro, Gulf of Boka Kotorska and their chemical analysis was performed. In seagrass C. nodosa, three phenolic compounds were identified (diosmetin 7-sulfate, caftaric and coutaric acid). The content of β-glucan, fatty acids, sterols and micro- and macro-elements were investigated among all samples. The highest content of β-glucan was detected in C. nodosa seagrass (13.04±0.42 g/100 g). The highest polyunsaturated fatty acids (PUFAs) level was reported in C. barbata, the brown alga (7.157 mg/g), which also had the significant sterol content (fucosterol, 21.76±0.1 μg/g). Green algae, C. bursa and H. tuna, showed the highest level of sterols (β-sitosterol, 95.21±0.16 μg/g and 73.90±0.08 μg/g, respectively). H. tuna had the highest content of calcium (Ca) in amount of 55125 μg/g. In C. bursa, C. barbata and C. nodosa, the Na/K ratio was low (0.43, 0.46 and 0.69, respectively).
PB  - Wiley
T2  - Chemistry and Biodiversity
T1  - Chemical Analysis of Selected Seaweeds and Seagrass from the Adriatic Coast of Montenegro
VL  - 16
IS  - 10
SP  - 1
EP  - 11
DO  - 10.1002/cbdv.201900327
ER  - 

@article{
author = "Milović, Sanja and Stanković, Ivan and Nikolić, Dejan and Radović, Jelena and Kolundžić, Marina and Nikolić, Vesna and Stanojković, Tatjana and Petović, Slavica and Kundaković-Vasović, Tatjana",
year = "2019",
abstract = "Three seaweeds (Halimeda tuna, Codium bursa and Cystoseira barbata) and one seagrass (Cymodocea nodosa) were collected from the Coast of Montenegro, Gulf of Boka Kotorska and their chemical analysis was performed. In seagrass C. nodosa, three phenolic compounds were identified (diosmetin 7-sulfate, caftaric and coutaric acid). The content of β-glucan, fatty acids, sterols and micro- and macro-elements were investigated among all samples. The highest content of β-glucan was detected in C. nodosa seagrass (13.04±0.42 g/100 g). The highest polyunsaturated fatty acids (PUFAs) level was reported in C. barbata, the brown alga (7.157 mg/g), which also had the significant sterol content (fucosterol, 21.76±0.1 μg/g). Green algae, C. bursa and H. tuna, showed the highest level of sterols (β-sitosterol, 95.21±0.16 μg/g and 73.90±0.08 μg/g, respectively). H. tuna had the highest content of calcium (Ca) in amount of 55125 μg/g. In C. bursa, C. barbata and C. nodosa, the Na/K ratio was low (0.43, 0.46 and 0.69, respectively).",
publisher = "Wiley",
journal = "Chemistry and Biodiversity",
title = "Chemical Analysis of Selected Seaweeds and Seagrass from the Adriatic Coast of Montenegro",
volume = "16",
number = "10",
pages = "1-11",
doi = "10.1002/cbdv.201900327"
}

Milović, S., Stanković, I., Nikolić, D., Radović, J., Kolundžić, M., Nikolić, V., Stanojković, T., Petović, S.,& Kundaković-Vasović, T.. (2019). Chemical Analysis of Selected Seaweeds and Seagrass from the Adriatic Coast of Montenegro. in Chemistry and Biodiversity
Wiley., 16(10), 1-11.
https://doi.org/10.1002/cbdv.201900327

Milović S, Stanković I, Nikolić D, Radović J, Kolundžić M, Nikolić V, Stanojković T, Petović S, Kundaković-Vasović T. Chemical Analysis of Selected Seaweeds and Seagrass from the Adriatic Coast of Montenegro. in Chemistry and Biodiversity. 2019;16(10):1-11.
doi:10.1002/cbdv.201900327 .

Milović, Sanja, Stanković, Ivan, Nikolić, Dejan, Radović, Jelena, Kolundžić, Marina, Nikolić, Vesna, Stanojković, Tatjana, Petović, Slavica, Kundaković-Vasović, Tatjana, "Chemical Analysis of Selected Seaweeds and Seagrass from the Adriatic Coast of Montenegro" in Chemistry and Biodiversity, 16, no. 10 (2019):1-11,
https://doi.org/10.1002/cbdv.201900327 . .

TY  - JOUR
AU  - Škobić, Slađana
AU  - Marčetić, Mirjana
AU  - Kundaković-Vasović, Tatjana
AU  - Crnobarac, Jovan
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3465
AB  - Sweet flag rhizome (Acorus calamus L., Araceae) exhibits various biological activities such as sedative, anticonvulsant,
immunosuppressant, antidiabetic, and anti-inflammatory. This plant is protected in Serbia, but due to
its high market requirements, the cultivation of this species could be the final solution. The content and composition
of essential oils from 24 cultivated samples and five samples of sweet flag from natural populations, as
well as the influence of nitrogen fertilization on the composition of essential oils were studied. Essential oils were
analysed using GC and GC/MS. Statistical analysis included the ANOVA and PCA analysis. The total content of
essential oil was not significantly different between the natural (0.80–1.10%) and cultivated samples
(1.15–1.30%). In all essential oils main compounds were β-asarone (4.82–17.98%), cadinadienol
(10.14–13.95%), camphor (3.55–13.20%), and acorenone (9.68–12.64%). The cultivation of sweet flag showed
significant (p < 0.05) impact on the composition of essential oil and the content of β-asarone (4.82–17.07%,
mean 11.95±4.76 vs. 17.51–17.98%, mean 17.71 ± 2.23). The PCA analysis also separated the essential oils
from natural and cultivated conditions based on the content of β-asarone. On the other hand, the amount of the
fertilizer did not show any effect (p > 0.05) on the composition of investigated samples. The process of cultivation
increased the level of active, but potentially genotoxic and carcinogenic compound β-asarone. It would be
significant to determine the content of β-asarone routinely in the sweet flag rhizome, especially in the cultivated
plants.
PB  - Elsevier
T2  - Industrial Crops & Products
T1  - Nitrogen fertilization and the essential oils profile of the rhizomes of different sweet flag populations (Acorus calamus L.)
VL  - 142
SP  - 1
EP  - 5
DO  - 10.1016/j.indcrop.2019.111871
ER  - 

@article{
author = "Škobić, Slađana and Marčetić, Mirjana and Kundaković-Vasović, Tatjana and Crnobarac, Jovan",
year = "2019",
abstract = "Sweet flag rhizome (Acorus calamus L., Araceae) exhibits various biological activities such as sedative, anticonvulsant,
immunosuppressant, antidiabetic, and anti-inflammatory. This plant is protected in Serbia, but due to
its high market requirements, the cultivation of this species could be the final solution. The content and composition
of essential oils from 24 cultivated samples and five samples of sweet flag from natural populations, as
well as the influence of nitrogen fertilization on the composition of essential oils were studied. Essential oils were
analysed using GC and GC/MS. Statistical analysis included the ANOVA and PCA analysis. The total content of
essential oil was not significantly different between the natural (0.80–1.10%) and cultivated samples
(1.15–1.30%). In all essential oils main compounds were β-asarone (4.82–17.98%), cadinadienol
(10.14–13.95%), camphor (3.55–13.20%), and acorenone (9.68–12.64%). The cultivation of sweet flag showed
significant (p < 0.05) impact on the composition of essential oil and the content of β-asarone (4.82–17.07%,
mean 11.95±4.76 vs. 17.51–17.98%, mean 17.71 ± 2.23). The PCA analysis also separated the essential oils
from natural and cultivated conditions based on the content of β-asarone. On the other hand, the amount of the
fertilizer did not show any effect (p > 0.05) on the composition of investigated samples. The process of cultivation
increased the level of active, but potentially genotoxic and carcinogenic compound β-asarone. It would be
significant to determine the content of β-asarone routinely in the sweet flag rhizome, especially in the cultivated
plants.",
publisher = "Elsevier",
journal = "Industrial Crops & Products",
title = "Nitrogen fertilization and the essential oils profile of the rhizomes of different sweet flag populations (Acorus calamus L.)",
volume = "142",
pages = "1-5",
doi = "10.1016/j.indcrop.2019.111871"
}

Škobić, S., Marčetić, M., Kundaković-Vasović, T.,& Crnobarac, J.. (2019). Nitrogen fertilization and the essential oils profile of the rhizomes of different sweet flag populations (Acorus calamus L.). in Industrial Crops & Products
Elsevier., 142, 1-5.
https://doi.org/10.1016/j.indcrop.2019.111871

Škobić S, Marčetić M, Kundaković-Vasović T, Crnobarac J. Nitrogen fertilization and the essential oils profile of the rhizomes of different sweet flag populations (Acorus calamus L.). in Industrial Crops & Products. 2019;142:1-5.
doi:10.1016/j.indcrop.2019.111871 .

Škobić, Slađana, Marčetić, Mirjana, Kundaković-Vasović, Tatjana, Crnobarac, Jovan, "Nitrogen fertilization and the essential oils profile of the rhizomes of different sweet flag populations (Acorus calamus L.)" in Industrial Crops & Products, 142 (2019):1-5,
https://doi.org/10.1016/j.indcrop.2019.111871 . .

TY  - JOUR
AU  - Milović, Sanja
AU  - Stanković, Ivan
AU  - Nikolić, Dejan
AU  - Radović, Jelena
AU  - Kolundžić, Marina
AU  - Nikolić, Vesna
AU  - Stanojković, Tatjana
AU  - Petović, Slavica
AU  - Kundaković-Vasović, Tatjana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3463
AB  - Three seaweeds (Halimeda tuna, Codium bursa and Cystoseira barbata) and one seagrass (Cymodocea nodosa) were collected from the Coast of Montenegro, Gulf of Boka Kotorska and their chemical analysis was performed. In seagrass C. nodosa, three phenolic compounds were identified (diosmetin 7-sulfate, caftaric and coutaric acid). The content of β-glucan, fatty acids, sterols and micro- and macro-elements were investigated among all samples. The highest content of β-glucan was detected in C. nodosa seagrass (13.04±0.42 g/100 g). The highest polyunsaturated fatty acids (PUFAs) level was reported in C. barbata, the brown alga (7.157 mg/g), which also had the significant sterol content (fucosterol, 21.76±0.1 μg/g). Green algae, C. bursa and H. tuna, showed the highest level of sterols (β-sitosterol, 95.21±0.16 μg/g and 73.90±0.08 μg/g, respectively). H. tuna had the highest content of calcium (Ca) in amount of 55125 μg/g. In C. bursa, C. barbata and C. nodosa, the Na/K ratio was low (0.43, 0.46 and 0.69, respectively).
PB  - Wiley
T2  - Chemistry and Biodiversity
T1  - Chemical Analysis of Selected Seaweeds and Seagrass from the Adriatic Coast of Montenegro
VL  - 16
IS  - 10
SP  - 1
EP  - 11
DO  - 10.1002/cbdv.201900327
ER  - 

@article{
author = "Milović, Sanja and Stanković, Ivan and Nikolić, Dejan and Radović, Jelena and Kolundžić, Marina and Nikolić, Vesna and Stanojković, Tatjana and Petović, Slavica and Kundaković-Vasović, Tatjana",
year = "2019",
abstract = "Three seaweeds (Halimeda tuna, Codium bursa and Cystoseira barbata) and one seagrass (Cymodocea nodosa) were collected from the Coast of Montenegro, Gulf of Boka Kotorska and their chemical analysis was performed. In seagrass C. nodosa, three phenolic compounds were identified (diosmetin 7-sulfate, caftaric and coutaric acid). The content of β-glucan, fatty acids, sterols and micro- and macro-elements were investigated among all samples. The highest content of β-glucan was detected in C. nodosa seagrass (13.04±0.42 g/100 g). The highest polyunsaturated fatty acids (PUFAs) level was reported in C. barbata, the brown alga (7.157 mg/g), which also had the significant sterol content (fucosterol, 21.76±0.1 μg/g). Green algae, C. bursa and H. tuna, showed the highest level of sterols (β-sitosterol, 95.21±0.16 μg/g and 73.90±0.08 μg/g, respectively). H. tuna had the highest content of calcium (Ca) in amount of 55125 μg/g. In C. bursa, C. barbata and C. nodosa, the Na/K ratio was low (0.43, 0.46 and 0.69, respectively).",
publisher = "Wiley",
journal = "Chemistry and Biodiversity",
title = "Chemical Analysis of Selected Seaweeds and Seagrass from the Adriatic Coast of Montenegro",
volume = "16",
number = "10",
pages = "1-11",
doi = "10.1002/cbdv.201900327"
}

Milović, S., Stanković, I., Nikolić, D., Radović, J., Kolundžić, M., Nikolić, V., Stanojković, T., Petović, S.,& Kundaković-Vasović, T.. (2019). Chemical Analysis of Selected Seaweeds and Seagrass from the Adriatic Coast of Montenegro. in Chemistry and Biodiversity
Wiley., 16(10), 1-11.
https://doi.org/10.1002/cbdv.201900327

Milović S, Stanković I, Nikolić D, Radović J, Kolundžić M, Nikolić V, Stanojković T, Petović S, Kundaković-Vasović T. Chemical Analysis of Selected Seaweeds and Seagrass from the Adriatic Coast of Montenegro. in Chemistry and Biodiversity. 2019;16(10):1-11.
doi:10.1002/cbdv.201900327 .

Milović, Sanja, Stanković, Ivan, Nikolić, Dejan, Radović, Jelena, Kolundžić, Marina, Nikolić, Vesna, Stanojković, Tatjana, Petović, Slavica, Kundaković-Vasović, Tatjana, "Chemical Analysis of Selected Seaweeds and Seagrass from the Adriatic Coast of Montenegro" in Chemistry and Biodiversity, 16, no. 10 (2019):1-11,
https://doi.org/10.1002/cbdv.201900327 . .

TY  - CONF
AU  - Milutinović, Violeta
AU  - Petrović, Predrag
AU  - Klaus, Anita
AU  - Ušjak, Ljuboš
AU  - Niketić, Marjan
AU  - Petrović, Silvana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5110
AB  - The ability of the dried MeOH extracts of aerial flowering parts of 28 Hieracium s. str. species from Balkan Peninsula and their selected metabolites (seven flavonoids, three phenolic acids and two sesquiterpene lactones) to inhibit acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) was determined using colorimetric Ellman method. The study included: H. gymnocephalum, H. orieni, H. blecicii, H. paratrichum, H. spirocaule, H. mokragorae, H. pannosum s.l., H. plumulosum, H. villosum, H. pilosum, H. pseudoschenkii, H. naegelianum, H. anastrum, H. calophyllum, H. scheppigianum, H. durmitoricum, H. guentheri-beckii, H. mirificissimum, H. coloriscapum, H. pyricephalum, H. albopellitum, H. glabratum, H. scorzonerifolium s.l., H. dentatum s.l., H. neilreichii, H. valdepilosum s.l., H. tommasinianum and H. macrodontoides. The extracts were mainly more active towards AChE, i.e. all inhibited more than 50% AChE, with H. pseudoschenkii extract being the most potent (IC50=0.64 mg/mL). Seven extracts reached 50% inhibition of 
BuChE, and H. pilosum extract was the most active (IC50=0.56 mg/mL). The observed activity could be attributed to some tested constituents. Flavonoid aglycones apigenin, luteolin and diosmetin significantly inhibited both enzymes (IC50AChE=47.12-89.89 μg/mL; C50BuChE=18.40-73.44 μg/mL). Sesquiterpene lactone 8-epiixerisamine A selectively inhibited AChE (IC50=80.01 μg/mL). The other tested metabolites did not reach 50% inhibition of both enzymes.
PB  - Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš
PB  - Institute for Nature Conservation of Serbia
C3  - 13th Symposium on the Flora of  Southeastern Serbia  and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts
T1  - Acetylcholinesterase and butyrylcholinesterase inhibitory activity of methanol extracts of 28 Hieracium species and their selected metabolites
SP  - 180
EP  - 180
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5110
ER  - 

@conference{
author = "Milutinović, Violeta and Petrović, Predrag and Klaus, Anita and Ušjak, Ljuboš and Niketić, Marjan and Petrović, Silvana",
year = "2019",
abstract = "The ability of the dried MeOH extracts of aerial flowering parts of 28 Hieracium s. str. species from Balkan Peninsula and their selected metabolites (seven flavonoids, three phenolic acids and two sesquiterpene lactones) to inhibit acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) was determined using colorimetric Ellman method. The study included: H. gymnocephalum, H. orieni, H. blecicii, H. paratrichum, H. spirocaule, H. mokragorae, H. pannosum s.l., H. plumulosum, H. villosum, H. pilosum, H. pseudoschenkii, H. naegelianum, H. anastrum, H. calophyllum, H. scheppigianum, H. durmitoricum, H. guentheri-beckii, H. mirificissimum, H. coloriscapum, H. pyricephalum, H. albopellitum, H. glabratum, H. scorzonerifolium s.l., H. dentatum s.l., H. neilreichii, H. valdepilosum s.l., H. tommasinianum and H. macrodontoides. The extracts were mainly more active towards AChE, i.e. all inhibited more than 50% AChE, with H. pseudoschenkii extract being the most potent (IC50=0.64 mg/mL). Seven extracts reached 50% inhibition of 
BuChE, and H. pilosum extract was the most active (IC50=0.56 mg/mL). The observed activity could be attributed to some tested constituents. Flavonoid aglycones apigenin, luteolin and diosmetin significantly inhibited both enzymes (IC50AChE=47.12-89.89 μg/mL; C50BuChE=18.40-73.44 μg/mL). Sesquiterpene lactone 8-epiixerisamine A selectively inhibited AChE (IC50=80.01 μg/mL). The other tested metabolites did not reach 50% inhibition of both enzymes.",
publisher = "Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, Institute for Nature Conservation of Serbia",
journal = "13th Symposium on the Flora of  Southeastern Serbia  and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts",
title = "Acetylcholinesterase and butyrylcholinesterase inhibitory activity of methanol extracts of 28 Hieracium species and their selected metabolites",
pages = "180-180",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5110"
}

Milutinović, V., Petrović, P., Klaus, A., Ušjak, L., Niketić, M.,& Petrović, S.. (2019). Acetylcholinesterase and butyrylcholinesterase inhibitory activity of methanol extracts of 28 Hieracium species and their selected metabolites. in 13th Symposium on the Flora of  Southeastern Serbia  and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts
Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš., 180-180.
https://hdl.handle.net/21.15107/rcub_farfar_5110

Milutinović V, Petrović P, Klaus A, Ušjak L, Niketić M, Petrović S. Acetylcholinesterase and butyrylcholinesterase inhibitory activity of methanol extracts of 28 Hieracium species and their selected metabolites. in 13th Symposium on the Flora of  Southeastern Serbia  and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts. 2019;:180-180.
https://hdl.handle.net/21.15107/rcub_farfar_5110 .

Milutinović, Violeta, Petrović, Predrag, Klaus, Anita, Ušjak, Ljuboš, Niketić, Marjan, Petrović, Silvana, "Acetylcholinesterase and butyrylcholinesterase inhibitory activity of methanol extracts of 28 Hieracium species and their selected metabolites" in 13th Symposium on the Flora of  Southeastern Serbia  and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts (2019):180-180,
https://hdl.handle.net/21.15107/rcub_farfar_5110 .

TY  - CONF
AU  - Milutinović, Violeta
AU  - Matić, Ivana
AU  - Grozdanić-Stanisavljević, Nađa
AU  - Stanojković, Tatjana
AU  - Soković, Marina
AU  - Ćirić, Ana
AU  - Niketić, Marjan
AU  - Petrović, Silvana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5109
AB  - Cytotoxic and antimicrobial activities were investigated for MeOH extracts of flowering aerial parts of Hieracium calophyllum R. Uechtr. (CAL), H. coloriscapum Rohlena & Zahn (COL), H. pseudoschenkii (Rohlena & Zahn) Niketić (PSE), H. valdepilosum Vill. s.l. (VAL) and H. glabratum Willd. (GLA), their two isolated sesquiterpene lactones 8-epiixerisamine A and crepiside E, as well as for CH2Cl2 extract of flowering aerial parts of H. scheppigianum Freyn (SCH). Crepiside E (IC50 = 46.00 μg/mL), COL (IC50 = 152.28 μg/mL) and SCH (IC50 = 148.12 μg/mL) showed 
the highest cytotoxic activity against HeLa cells in MTT test. The cytotoxic activity against LS174, A549, as well as against normal MRC-5 cells was significantly weaker. In microdilution test, crepiside E and 8-epiixerisamine A exhibited noteworthy antifungal effect against Aspergillus ochraceus, Penicillium funiculosum, Candida albicans and C. krusei (MIC = 0.15 - 0.4 mg/mL, MFC = 0.3 - 0.8 mg/mL). Among the extracts, the best antibacterial activity was shown by SCH and CAL against Pseudomonas aeruginosa (MIC = 1.68 and 2.52 mg/mL, MBC = 3.36 and 5.04 mg/mL), and the highest antifungal activity by SCH and VAL against C. albicans (MIC = 2.48 and 2.52 mg/mL, MFC = 4.96 and 5.04 mg/mL).
PB  - Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš
PB  - Institute for Nature Conservation of Serbia
C3  - 13th Symposium on the Flora of  Southeastern Serbia  and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts
T1  - Cytotoxic and antimicrobial activity of selected Hieracium L. extracts and isolated sesquiterpene lactones
SP  - 162
EP  - 163
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5109
ER  - 

@conference{
author = "Milutinović, Violeta and Matić, Ivana and Grozdanić-Stanisavljević, Nađa and Stanojković, Tatjana and Soković, Marina and Ćirić, Ana and Niketić, Marjan and Petrović, Silvana",
year = "2019",
abstract = "Cytotoxic and antimicrobial activities were investigated for MeOH extracts of flowering aerial parts of Hieracium calophyllum R. Uechtr. (CAL), H. coloriscapum Rohlena & Zahn (COL), H. pseudoschenkii (Rohlena & Zahn) Niketić (PSE), H. valdepilosum Vill. s.l. (VAL) and H. glabratum Willd. (GLA), their two isolated sesquiterpene lactones 8-epiixerisamine A and crepiside E, as well as for CH2Cl2 extract of flowering aerial parts of H. scheppigianum Freyn (SCH). Crepiside E (IC50 = 46.00 μg/mL), COL (IC50 = 152.28 μg/mL) and SCH (IC50 = 148.12 μg/mL) showed 
the highest cytotoxic activity against HeLa cells in MTT test. The cytotoxic activity against LS174, A549, as well as against normal MRC-5 cells was significantly weaker. In microdilution test, crepiside E and 8-epiixerisamine A exhibited noteworthy antifungal effect against Aspergillus ochraceus, Penicillium funiculosum, Candida albicans and C. krusei (MIC = 0.15 - 0.4 mg/mL, MFC = 0.3 - 0.8 mg/mL). Among the extracts, the best antibacterial activity was shown by SCH and CAL against Pseudomonas aeruginosa (MIC = 1.68 and 2.52 mg/mL, MBC = 3.36 and 5.04 mg/mL), and the highest antifungal activity by SCH and VAL against C. albicans (MIC = 2.48 and 2.52 mg/mL, MFC = 4.96 and 5.04 mg/mL).",
publisher = "Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, Institute for Nature Conservation of Serbia",
journal = "13th Symposium on the Flora of  Southeastern Serbia  and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts",
title = "Cytotoxic and antimicrobial activity of selected Hieracium L. extracts and isolated sesquiterpene lactones",
pages = "162-163",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5109"
}

Milutinović, V., Matić, I., Grozdanić-Stanisavljević, N., Stanojković, T., Soković, M., Ćirić, A., Niketić, M.,& Petrović, S.. (2019). Cytotoxic and antimicrobial activity of selected Hieracium L. extracts and isolated sesquiterpene lactones. in 13th Symposium on the Flora of  Southeastern Serbia  and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts
Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš., 162-163.
https://hdl.handle.net/21.15107/rcub_farfar_5109

Milutinović V, Matić I, Grozdanić-Stanisavljević N, Stanojković T, Soković M, Ćirić A, Niketić M, Petrović S. Cytotoxic and antimicrobial activity of selected Hieracium L. extracts and isolated sesquiterpene lactones. in 13th Symposium on the Flora of  Southeastern Serbia  and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts. 2019;:162-163.
https://hdl.handle.net/21.15107/rcub_farfar_5109 .

Milutinović, Violeta, Matić, Ivana, Grozdanić-Stanisavljević, Nađa, Stanojković, Tatjana, Soković, Marina, Ćirić, Ana, Niketić, Marjan, Petrović, Silvana, "Cytotoxic and antimicrobial activity of selected Hieracium L. extracts and isolated sesquiterpene lactones" in 13th Symposium on the Flora of  Southeastern Serbia  and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts (2019):162-163,
https://hdl.handle.net/21.15107/rcub_farfar_5109 .

TY  - THES
AU  - Ušjak, Ljuboš
PY  - 2019
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=6810
UR  - http://nardus.mpn.gov.rs/handle/123456789/11249
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:19901/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=2048355682
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3696
AB  - Predmet istraņivanja doktorske disertacije je devet taksona roda Heracleum (Apiaceae): H. sphondylium, H. sibiricum, H. montanum, H. ternatum, H. pyrenaicum subsp. pollinianum, H. pyrenaicum subsp. orsinii i H. verticillatum iz grupe H. sphondylium, i H. orphanidis, svi iz (tipske) sect. Heracleum, kao i H. austriacum subsp. siifolium iz sect. Wendia.Gasnom hromatografijom sa FID i MS detekcijom analiziran je sastav etarskih ulja izolovanih destilacijom vodenom parom iz podzemnih organa, listova, cvasti i plodova. U uljima podzemnih organa dominirali su monoterpeni i/ili fenilpropani (taksoni grupe H. sphondylium), ili (Z)-falkarinol (H. orphanidis i H. austriacum); u uljima plodova alifatiĉni estri oktilacetat (sect. Heracleum) ili oktilheksanoat (H. austriacum), a u uljima listova i cvasti alifatiĉni estri (H. orphanidis) ili seskviterpeni i/ili fenilpropani. S obzirom da je u pojedinim uljima utvrĊeno prisustvo furanokumarina koji mogu delovati fototoksiĉno, definisan je bezbednosni profil ulja, tj. utvrĊen njihov maksimalni dozvoljeni dnevni unos.U dihlormetanskim ekstraktima podzemnih organa i plodova svih taksona identifikovano je ukupno 12 furanokumarina (LC-MS metodom pomoću standardnih supstanci ili na osnovu UV, MS, 1H i ROESY NMR spektara), od kojih su dominantni bili pimpinelin, bergapten, biakangelikol, heraklenin i/ili imperatorin.Hemosistematski znaĉaj komponenti etarskih ulja (u sluĉaju podzemnih organa i plodova i komponenti njihovih headspace frakcija) i furanokumarina utvrĊen je multivarijantnom statistiĉkom analizom (PCA, nMDS i UPGMA).U uljastim supernatantima dihlormetanskih ekstrakata plodova gasnom hromatografijom (GC-FID i GC-MS) analizirane su masne kiseline (dominantna je bila petroselinska), steroli (najzastupljeniji je bio β-sitosterol) i triterpeni.Antimikrobna aktivnost etarskih ulja pokazana je mikrodilucionom metodom na po osam sojeva bakterija i mikromiceta (u nekim sluĉajevima MIK su bile niņe ili bliske 0,1 mg/mL); selektivna citotoksiĉnost MTT testom na tumorske HeLa, LS174 i A549 ćelije (u nekim sluĉajevima IC50 su bile niņe ili bliske 30 μg/mL; ulja uglavnom nisuinhibirala rast normalnih MRC-5 ćelija)...
AB  - This    doctoral    dissertation focuses    on    nineHeracleumtaxa (Apiaceae): H.  sphondylium, H. sibiricum, H. montanum, H. ternatum, H. pyrenaicumsubsp. pollinianum, H. pyrenaicumsubsp. orsiniiandH. verticillatum,  belonging  togroupH.  sphondylium, andH. orphanidis, all  from  the (type)sect. Heracleum, as  well  asH.austriacum subsp.siifoliumfromsect. Wendia. By   GC-FID   and   GC-MS,   the   composition   of   essential   oils isolated by hydrodistillation fromunderground  parts,  leaves,  flowers  and  fruits  was  analysed. Underground  parts  oils  were  dominated  bymonoterpenesand/orphenylpropanoids (H. sphondyliumgroup), or(Z)-falcarinol (H. orphanidisandH. austriacum); fruit oils byaliphatic  estersoctylacetate(sect. Heracleum) oroctylhexanoate(H.  austriacum), andleaf  and  flower  oilsby  aliphatic  esters(H.  orphanidis) or  sesquiterpenesand/orphenylpropanoids. Considering   that   in   some   of   these   oils   potentially   phototoxic furanocoumarins  were  detected, safety  profileof  the  oils,  i.e.  their  maximum  daily intake wasestimated.In dichloromethane extracts of underground parts and fruits of investigated taxa,atotal  of 12  furanocoumarins  were  identified(by LC-MS using standard  compoundsorbased onUV, MS, 1H andROESY NMR spectra), among which pimpinellin, bergapten, byakangelicol, heraclenin and/orimperatorinwere dominant. Chemosystematicsignificance   ofessential   oil   components(in   the   case   ofunderground  parts  and  fruits,  of  their headspacefractioncomponents as  well) and furanocoumarinswas demonstrated  usingmultivariatestatistics  (PCA,  nMDS  andUPGMA).In  oily  supernatants  of  fruit dichloromethaneextracts,  by GC-FID and  GC-MS,fatty acids (petroselinic acid was dominant), sterols (β-sitosterolwas the most abundant) and triterpeneswere analysed. Antimicrobialactivity of the essential oilswas showedbymicrodilutionmethodoneightbacteria and eightfungi(in some cases MICs werelower or around0.1 mg/mL); selective cytotoxicityby  MTT  teston cancerHeLa,  LS174 andA549 cells(in  some 
cases IC50werelower or around 30 μg/mL; oils mostly did not inhibit growth ofnormalMRC-5 cells). Essential  oils  showed  weakanti-DPPH  potential. The  composition  of their active zones in TLC-DPPH test was analysed byGC-FID and GC-MS(oxygenated terpenes and phenylpropanoidswere dominant).
PB  - Универзитет у Београду, Фармацеутски факултет
T2  - Универзитет у Београду
T1  - Hemijska i farmakološka karakterizacija odabranih taksona roda Heracleum L. (Apiaceae), autohtonih za jugoistočnu Evropu
UR  - https://hdl.handle.net/21.15107/rcub_nardus_11249
ER  - 

@phdthesis{
author = "Ušjak, Ljuboš",
year = "2019",
abstract = "Predmet istraņivanja doktorske disertacije je devet taksona roda Heracleum (Apiaceae): H. sphondylium, H. sibiricum, H. montanum, H. ternatum, H. pyrenaicum subsp. pollinianum, H. pyrenaicum subsp. orsinii i H. verticillatum iz grupe H. sphondylium, i H. orphanidis, svi iz (tipske) sect. Heracleum, kao i H. austriacum subsp. siifolium iz sect. Wendia.Gasnom hromatografijom sa FID i MS detekcijom analiziran je sastav etarskih ulja izolovanih destilacijom vodenom parom iz podzemnih organa, listova, cvasti i plodova. U uljima podzemnih organa dominirali su monoterpeni i/ili fenilpropani (taksoni grupe H. sphondylium), ili (Z)-falkarinol (H. orphanidis i H. austriacum); u uljima plodova alifatiĉni estri oktilacetat (sect. Heracleum) ili oktilheksanoat (H. austriacum), a u uljima listova i cvasti alifatiĉni estri (H. orphanidis) ili seskviterpeni i/ili fenilpropani. S obzirom da je u pojedinim uljima utvrĊeno prisustvo furanokumarina koji mogu delovati fototoksiĉno, definisan je bezbednosni profil ulja, tj. utvrĊen njihov maksimalni dozvoljeni dnevni unos.U dihlormetanskim ekstraktima podzemnih organa i plodova svih taksona identifikovano je ukupno 12 furanokumarina (LC-MS metodom pomoću standardnih supstanci ili na osnovu UV, MS, 1H i ROESY NMR spektara), od kojih su dominantni bili pimpinelin, bergapten, biakangelikol, heraklenin i/ili imperatorin.Hemosistematski znaĉaj komponenti etarskih ulja (u sluĉaju podzemnih organa i plodova i komponenti njihovih headspace frakcija) i furanokumarina utvrĊen je multivarijantnom statistiĉkom analizom (PCA, nMDS i UPGMA).U uljastim supernatantima dihlormetanskih ekstrakata plodova gasnom hromatografijom (GC-FID i GC-MS) analizirane su masne kiseline (dominantna je bila petroselinska), steroli (najzastupljeniji je bio β-sitosterol) i triterpeni.Antimikrobna aktivnost etarskih ulja pokazana je mikrodilucionom metodom na po osam sojeva bakterija i mikromiceta (u nekim sluĉajevima MIK su bile niņe ili bliske 0,1 mg/mL); selektivna citotoksiĉnost MTT testom na tumorske HeLa, LS174 i A549 ćelije (u nekim sluĉajevima IC50 su bile niņe ili bliske 30 μg/mL; ulja uglavnom nisuinhibirala rast normalnih MRC-5 ćelija)..., This    doctoral    dissertation focuses    on    nineHeracleumtaxa (Apiaceae): H.  sphondylium, H. sibiricum, H. montanum, H. ternatum, H. pyrenaicumsubsp. pollinianum, H. pyrenaicumsubsp. orsiniiandH. verticillatum,  belonging  togroupH.  sphondylium, andH. orphanidis, all  from  the (type)sect. Heracleum, as  well  asH.austriacum subsp.siifoliumfromsect. Wendia. By   GC-FID   and   GC-MS,   the   composition   of   essential   oils isolated by hydrodistillation fromunderground  parts,  leaves,  flowers  and  fruits  was  analysed. Underground  parts  oils  were  dominated  bymonoterpenesand/orphenylpropanoids (H. sphondyliumgroup), or(Z)-falcarinol (H. orphanidisandH. austriacum); fruit oils byaliphatic  estersoctylacetate(sect. Heracleum) oroctylhexanoate(H.  austriacum), andleaf  and  flower  oilsby  aliphatic  esters(H.  orphanidis) or  sesquiterpenesand/orphenylpropanoids. Considering   that   in   some   of   these   oils   potentially   phototoxic furanocoumarins  were  detected, safety  profileof  the  oils,  i.e.  their  maximum  daily intake wasestimated.In dichloromethane extracts of underground parts and fruits of investigated taxa,atotal  of 12  furanocoumarins  were  identified(by LC-MS using standard  compoundsorbased onUV, MS, 1H andROESY NMR spectra), among which pimpinellin, bergapten, byakangelicol, heraclenin and/orimperatorinwere dominant. Chemosystematicsignificance   ofessential   oil   components(in   the   case   ofunderground  parts  and  fruits,  of  their headspacefractioncomponents as  well) and furanocoumarinswas demonstrated  usingmultivariatestatistics  (PCA,  nMDS  andUPGMA).In  oily  supernatants  of  fruit dichloromethaneextracts,  by GC-FID and  GC-MS,fatty acids (petroselinic acid was dominant), sterols (β-sitosterolwas the most abundant) and triterpeneswere analysed. Antimicrobialactivity of the essential oilswas showedbymicrodilutionmethodoneightbacteria and eightfungi(in some cases MICs werelower or around0.1 mg/mL); selective cytotoxicityby  MTT  teston cancerHeLa,  LS174 andA549 cells(in  some 
cases IC50werelower or around 30 μg/mL; oils mostly did not inhibit growth ofnormalMRC-5 cells). Essential  oils  showed  weakanti-DPPH  potential. The  composition  of their active zones in TLC-DPPH test was analysed byGC-FID and GC-MS(oxygenated terpenes and phenylpropanoidswere dominant).",
publisher = "Универзитет у Београду, Фармацеутски факултет",
journal = "Универзитет у Београду",
title = "Hemijska i farmakološka karakterizacija odabranih taksona roda Heracleum L. (Apiaceae), autohtonih za jugoistočnu Evropu",
url = "https://hdl.handle.net/21.15107/rcub_nardus_11249"
}

Ušjak, L.. (2019). Hemijska i farmakološka karakterizacija odabranih taksona roda Heracleum L. (Apiaceae), autohtonih za jugoistočnu Evropu. in Универзитет у Београду
Универзитет у Београду, Фармацеутски факултет..
https://hdl.handle.net/21.15107/rcub_nardus_11249

Ušjak L. Hemijska i farmakološka karakterizacija odabranih taksona roda Heracleum L. (Apiaceae), autohtonih za jugoistočnu Evropu. in Универзитет у Београду. 2019;.
https://hdl.handle.net/21.15107/rcub_nardus_11249 .

Ušjak, Ljuboš, "Hemijska i farmakološka karakterizacija odabranih taksona roda Heracleum L. (Apiaceae), autohtonih za jugoistočnu Evropu" in Универзитет у Београду (2019),
https://hdl.handle.net/21.15107/rcub_nardus_11249 .

TY  - CONF
AU  - Ušjak, Ljuboš
AU  - Drobac, Milica
AU  - Niketić, Marjan
AU  - Petrović, Silvana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5238
AB  - Headspace fractions of fruits and/or roots of Heracleum sphondylium, H. sibiricum, H. montanum, H. ternatum, H. pyrenaicum subsp. pollinianum, H. pyrenaicum subsp. orsinii and H. verticillatum, belonging to group H. sphondylium, and H. orphanidis, all from sect. Heracleum, as well as H. austriacum subsp. siifolium from sect. Wendia (from Serbia, Montenegro, North Macedonia or Slovenia) were isolated using automatic headspace sampler and analyzed by GC-FID and GC-MS. Chemosystematic significance of identified constituents was evaluated using multivariate statistics: PCA, nMDS and UPGMA. GC analysis of headspace fractions from 17 fruit samples of all investigated taxa showed that octyl acetate or α-pinene (sect. Heracleum), or n-octanol (H. austriacum) were the most abundant. The analysis of the fractions from 13 root samples of investigated taxa belonging to sect. Heracleum revealed prevalence of β-pinene or (Z)-β-ocimene (group H. sphondylium), or n-nonane (H. orphanidis). Statistical analysis of fruit fractions revealed separation of H. austriacum and H. orphanidis from investigated members of group H. sphondylium. Morphologically related H. sphondylium and H. montanum were grouped together and H. verticillatum was well separated from other representatives of group H. sphondylium. Complete separation of H. sibiricum, H. ternatum and H. pyrenaicum samples was noticed in root fractions statistical analysis.
PB  - Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš
PB  - Institute for Nature Conservation of Serbia
C3  - 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts
T1  - Chemical composition of headspace fractions from fruits and roots of selected Heracleum taxa collected in Southeastern Europe and its chemosystematic significance
SP  - 167
EP  - 167
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5238
ER  - 

@conference{
author = "Ušjak, Ljuboš and Drobac, Milica and Niketić, Marjan and Petrović, Silvana",
year = "2019",
abstract = "Headspace fractions of fruits and/or roots of Heracleum sphondylium, H. sibiricum, H. montanum, H. ternatum, H. pyrenaicum subsp. pollinianum, H. pyrenaicum subsp. orsinii and H. verticillatum, belonging to group H. sphondylium, and H. orphanidis, all from sect. Heracleum, as well as H. austriacum subsp. siifolium from sect. Wendia (from Serbia, Montenegro, North Macedonia or Slovenia) were isolated using automatic headspace sampler and analyzed by GC-FID and GC-MS. Chemosystematic significance of identified constituents was evaluated using multivariate statistics: PCA, nMDS and UPGMA. GC analysis of headspace fractions from 17 fruit samples of all investigated taxa showed that octyl acetate or α-pinene (sect. Heracleum), or n-octanol (H. austriacum) were the most abundant. The analysis of the fractions from 13 root samples of investigated taxa belonging to sect. Heracleum revealed prevalence of β-pinene or (Z)-β-ocimene (group H. sphondylium), or n-nonane (H. orphanidis). Statistical analysis of fruit fractions revealed separation of H. austriacum and H. orphanidis from investigated members of group H. sphondylium. Morphologically related H. sphondylium and H. montanum were grouped together and H. verticillatum was well separated from other representatives of group H. sphondylium. Complete separation of H. sibiricum, H. ternatum and H. pyrenaicum samples was noticed in root fractions statistical analysis.",
publisher = "Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, Institute for Nature Conservation of Serbia",
journal = "13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts",
title = "Chemical composition of headspace fractions from fruits and roots of selected Heracleum taxa collected in Southeastern Europe and its chemosystematic significance",
pages = "167-167",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5238"
}

Ušjak, L., Drobac, M., Niketić, M.,& Petrović, S.. (2019). Chemical composition of headspace fractions from fruits and roots of selected Heracleum taxa collected in Southeastern Europe and its chemosystematic significance. in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts
Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš., 167-167.
https://hdl.handle.net/21.15107/rcub_farfar_5238

Ušjak L, Drobac M, Niketić M, Petrović S. Chemical composition of headspace fractions from fruits and roots of selected Heracleum taxa collected in Southeastern Europe and its chemosystematic significance. in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts. 2019;:167-167.
https://hdl.handle.net/21.15107/rcub_farfar_5238 .

Ušjak, Ljuboš, Drobac, Milica, Niketić, Marjan, Petrović, Silvana, "Chemical composition of headspace fractions from fruits and roots of selected Heracleum taxa collected in Southeastern Europe and its chemosystematic significance" in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts (2019):167-167,
https://hdl.handle.net/21.15107/rcub_farfar_5238 .

TY  - CHAP
AU  - Maksimović, Zoran
AU  - Samardžić, Stevan
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5262
AB  - Osteoarthritis (OA) is the most common form of arthritis, which represents a substantial economic burden for society and significantly affects patients’ quality of life. Current conventional treatments of OA may be insufficiently effective and unsafe. In an attempt to overcome these limitations, many patients use herbal medicinal products (HMPs) and dietary supplements. A considerable number of herbal drugs and preparations (e.g., willow bark, Salicis cortex; devil’s claw root, Harpagophyti radix; blackcurrant leaf, Ribis nigri folium; nettle leaf/herb, Urticae folium/herba; meadowsweet/meadowsweet flower, Filipendulae ulmariae herba/flos; rosemary leaf/oil, Rosmarini folium/aetheroleum; and juniper oil, Juniperi aetheroleum) are traditionally employed to relieve minor articular pain. Active constituents (e.g., sesquiterpene lactones, triterpenic acids, diarylheptanoids, iridoid glycosides, phenolic glycosides, procyanidins, and alkaloids) are not often fully known. Experimental studies suggest that herbal extracts/compounds are able to suppress inflammation, inhibit catabolic processes, and stimulate anabolic processes relevant to OA. Therapeutic benefit of most HMPs is expected solely from the experience of their long-standing traditional use. Efficacy and safety of several HMPs were assessed in clinical trials. The growing body of preclinical and clinical evidence provides rationale for the use of herbal products in the treatment of OA. However, at present, they cannot be recommended to patients with confidence.
PB  - IntechOpen, London, United Kingdom
T2  - Osteoarthritis Biomarkers and Treatments
T1  - Herbal Medicinal Products in the Treatment of Osteoarthritis
VL  - 2
SP  - 57
EP  - 74
DO  - 10.5772/intechopen.80593
ER  - 

@inbook{
author = "Maksimović, Zoran and Samardžić, Stevan",
year = "2019",
abstract = "Osteoarthritis (OA) is the most common form of arthritis, which represents a substantial economic burden for society and significantly affects patients’ quality of life. Current conventional treatments of OA may be insufficiently effective and unsafe. In an attempt to overcome these limitations, many patients use herbal medicinal products (HMPs) and dietary supplements. A considerable number of herbal drugs and preparations (e.g., willow bark, Salicis cortex; devil’s claw root, Harpagophyti radix; blackcurrant leaf, Ribis nigri folium; nettle leaf/herb, Urticae folium/herba; meadowsweet/meadowsweet flower, Filipendulae ulmariae herba/flos; rosemary leaf/oil, Rosmarini folium/aetheroleum; and juniper oil, Juniperi aetheroleum) are traditionally employed to relieve minor articular pain. Active constituents (e.g., sesquiterpene lactones, triterpenic acids, diarylheptanoids, iridoid glycosides, phenolic glycosides, procyanidins, and alkaloids) are not often fully known. Experimental studies suggest that herbal extracts/compounds are able to suppress inflammation, inhibit catabolic processes, and stimulate anabolic processes relevant to OA. Therapeutic benefit of most HMPs is expected solely from the experience of their long-standing traditional use. Efficacy and safety of several HMPs were assessed in clinical trials. The growing body of preclinical and clinical evidence provides rationale for the use of herbal products in the treatment of OA. However, at present, they cannot be recommended to patients with confidence.",
publisher = "IntechOpen, London, United Kingdom",
journal = "Osteoarthritis Biomarkers and Treatments",
booktitle = "Herbal Medicinal Products in the Treatment of Osteoarthritis",
volume = "2",
pages = "57-74",
doi = "10.5772/intechopen.80593"
}

Maksimović, Z.,& Samardžić, S.. (2019). Herbal Medicinal Products in the Treatment of Osteoarthritis. in Osteoarthritis Biomarkers and Treatments
IntechOpen, London, United Kingdom., 2, 57-74.
https://doi.org/10.5772/intechopen.80593

Maksimović Z, Samardžić S. Herbal Medicinal Products in the Treatment of Osteoarthritis. in Osteoarthritis Biomarkers and Treatments. 2019;2:57-74.
doi:10.5772/intechopen.80593 .

Maksimović, Zoran, Samardžić, Stevan, "Herbal Medicinal Products in the Treatment of Osteoarthritis" in Osteoarthritis Biomarkers and Treatments, 2 (2019):57-74,
https://doi.org/10.5772/intechopen.80593 . .