TY  - JOUR
AU  - Pavlović, Miloš
AU  - Đukić, Mirjana
AU  - Vojvodić, Danilo
AU  - Ninković, Milica
AU  - Stevanović, Ivana
AU  - Đurić, Ana
AU  - Stanojević, Boban
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3956
AB  - Background/Aim. Exposure  of  living  organisms  to  γ- radiation results in the overproduction of free radicals. The  aim of the study was to test if the subacute administration  of micronized zeolite (MZC) accomplishes radioprotective  role based on the evaluation of the status of oxidative stress  (OS) in the brain and 8-hydroxyguanosine (8-OH-dG)  in  the plasma of the rats exposed to the single γ-ray irradiation  of 2 and/or 10 Gray (Gy). Methods. Wistar rats were on a  four-week normal or 5% MZC supplemented diet and af- terward exposed to the single γ-ray irradiation  of 2 and 10  Gy. Groups of rats were: a) on a normal diet (the control  group, and 2Gy and 10Gy groups); b) on 5% MZC supple- mented diet (the control group –  MZC, MZC  +  2Gy, and  MZC + 10Gy  groups).  We  measured  malondialdehyde  (MDA), glutathione (GSH) total, and activity of total super- oxide  dismutase  (tSOD)  and  manganese  superoxide  dis- mutase (MnSOD)  in vulnerable brain regions (cerebellum,  hippocampus, and cerebral cortex) and 8-OH-dG in plasma.  Results.  Lower MDA was found in the MZC+2Gy and  MZC+10Gy  groups  compared  to  the  2Gy  and  10Gy  groups.  Activity  od  total  SOD  was  higher  in  the  MZC+10Gy group  than in the 10Gy  one. GSH was the  highest in the 10Gy group. Compared to the control group,  8-OH-dG was extremely higher in groups radiated with 10  Gy regardless of a diet, but slightly lower in the MZC+2Gy  and 2Gy groups. Conclusion. Subacute MZC pretreatment  accomplished partial radioprotective effect in irradiated rats  compared to non-irradiated rats, based on suppressed SOD  activity at 2 Gy, and reduced brain MDA when exposed to 2  Gy and 10 Gy.
AB  - Uvod/Cilj.  Izlaganje živih organizama gama zračenju re- zultira hiperprodukcijom slobodnih radikala. Cilj istraživanja  je bio da se ispita da li subakutna ishrana  dopunjena  sa 5%  mikronizovanog zeolita (MZC) ispoljava radiozaštitnu ulogu  na osnovu statusa oksidativnog stresa (OS) u mozgu i 8- hidroksiguanozina  (8-OH-dG)  u  plazmi  pacova  izloženih  pojedinačnim dozama jonizujućeg zračenja od 2 i 10 Gray  (Gy).  Metode.  Wistar  pacovi  su  bili  na  četvoronedeljnoj  normalnoj ishrani ili ishrani obogaćenoj sa 5% MZC, posle  čega su bili izloženi pojedinačnom jonizujućem zračenju od  2  Gy,  odnosno  10  Gy.  Grupe  pacova  bile  su:  a)  gru pa  pacova na normalnoj ishrani (kontrolna grupa i grupe 2Gy i  10Gy); b) grupa pacova na ishrani obogaćenoj sa 5%  MZC  (kontrolna  grupa  – MZC  i  grupe  MZC+2Gy  i   MZC+10Gy).  Meren  je  malondialdehid  (MDA),  glutation  (GSH)  i  aktivnost  ukupne  (tSOD)  i  mangan  superoksid  dizmutaze  (MnSOD)  u  osetljivim  strukturama  mozga  (cerebelum, hipokampus i cerebralni korteks), a 8-OH-dG u  plazmi.  Rezultati. Biomarker  MDA  je  bio  niži  u  MZC+2Gy i MZC+10Gy grupama, u odnosu na grupe 2Gy  i  10Gy.  Aktivnost  ukupne SOD  je  bila  viša  u  grupi  MZC+10Gy, u odnosu na grupu 10Gy. Najviši nivo GSH  bio je u grupi 10Gy. U pređenju sa kontrolnom grupom, 8- OH-dG je bio izuzetno viši u grupama ozračenim sa 10 Gy,  bez obzira na dijetetski režim i niži u grupama MZC+2Gy i  2Gy.  Zaključak. Pacovi  koji  su  bili  na  režimu  ishrane  obogaćene  sa  5%  MZC  bili  su  delimično  zaštićeni  od  zračenja, shodno redukovanoj moždanoj aktivnosti SOD pri  2  Gy i sniženom nivou MDA pri izlaganju zračenju od 2 i  10 Gy.
PB  - Belgrade: Military Medical Academy, INI
T2  - Vojnosanitetski pregled
T1  - Moderate radioprotective role of zeolite in rats
T1  - Umerena radioprotektivna uloga zeolita kod pacova
VL  - 78
IS  - 7
SP  - 760
EP  - 768
DO  - 10.2298/VSP190702136P
ER  - 

@article{
author = "Pavlović, Miloš and Đukić, Mirjana and Vojvodić, Danilo and Ninković, Milica and Stevanović, Ivana and Đurić, Ana and Stanojević, Boban",
year = "2021",
abstract = "Background/Aim. Exposure  of  living  organisms  to  γ- radiation results in the overproduction of free radicals. The  aim of the study was to test if the subacute administration  of micronized zeolite (MZC) accomplishes radioprotective  role based on the evaluation of the status of oxidative stress  (OS) in the brain and 8-hydroxyguanosine (8-OH-dG)  in  the plasma of the rats exposed to the single γ-ray irradiation  of 2 and/or 10 Gray (Gy). Methods. Wistar rats were on a  four-week normal or 5% MZC supplemented diet and af- terward exposed to the single γ-ray irradiation  of 2 and 10  Gy. Groups of rats were: a) on a normal diet (the control  group, and 2Gy and 10Gy groups); b) on 5% MZC supple- mented diet (the control group –  MZC, MZC  +  2Gy, and  MZC + 10Gy  groups).  We  measured  malondialdehyde  (MDA), glutathione (GSH) total, and activity of total super- oxide  dismutase  (tSOD)  and  manganese  superoxide  dis- mutase (MnSOD)  in vulnerable brain regions (cerebellum,  hippocampus, and cerebral cortex) and 8-OH-dG in plasma.  Results.  Lower MDA was found in the MZC+2Gy and  MZC+10Gy  groups  compared  to  the  2Gy  and  10Gy  groups.  Activity  od  total  SOD  was  higher  in  the  MZC+10Gy group  than in the 10Gy  one. GSH was the  highest in the 10Gy group. Compared to the control group,  8-OH-dG was extremely higher in groups radiated with 10  Gy regardless of a diet, but slightly lower in the MZC+2Gy  and 2Gy groups. Conclusion. Subacute MZC pretreatment  accomplished partial radioprotective effect in irradiated rats  compared to non-irradiated rats, based on suppressed SOD  activity at 2 Gy, and reduced brain MDA when exposed to 2  Gy and 10 Gy., Uvod/Cilj.  Izlaganje živih organizama gama zračenju re- zultira hiperprodukcijom slobodnih radikala. Cilj istraživanja  je bio da se ispita da li subakutna ishrana  dopunjena  sa 5%  mikronizovanog zeolita (MZC) ispoljava radiozaštitnu ulogu  na osnovu statusa oksidativnog stresa (OS) u mozgu i 8- hidroksiguanozina  (8-OH-dG)  u  plazmi  pacova  izloženih  pojedinačnim dozama jonizujućeg zračenja od 2 i 10 Gray  (Gy).  Metode.  Wistar  pacovi  su  bili  na  četvoronedeljnoj  normalnoj ishrani ili ishrani obogaćenoj sa 5% MZC, posle  čega su bili izloženi pojedinačnom jonizujućem zračenju od  2  Gy,  odnosno  10  Gy.  Grupe  pacova  bile  su:  a)  gru pa  pacova na normalnoj ishrani (kontrolna grupa i grupe 2Gy i  10Gy); b) grupa pacova na ishrani obogaćenoj sa 5%  MZC  (kontrolna  grupa  – MZC  i  grupe  MZC+2Gy  i   MZC+10Gy).  Meren  je  malondialdehid  (MDA),  glutation  (GSH)  i  aktivnost  ukupne  (tSOD)  i  mangan  superoksid  dizmutaze  (MnSOD)  u  osetljivim  strukturama  mozga  (cerebelum, hipokampus i cerebralni korteks), a 8-OH-dG u  plazmi.  Rezultati. Biomarker  MDA  je  bio  niži  u  MZC+2Gy i MZC+10Gy grupama, u odnosu na grupe 2Gy  i  10Gy.  Aktivnost  ukupne SOD  je  bila  viša  u  grupi  MZC+10Gy, u odnosu na grupu 10Gy. Najviši nivo GSH  bio je u grupi 10Gy. U pređenju sa kontrolnom grupom, 8- OH-dG je bio izuzetno viši u grupama ozračenim sa 10 Gy,  bez obzira na dijetetski režim i niži u grupama MZC+2Gy i  2Gy.  Zaključak. Pacovi  koji  su  bili  na  režimu  ishrane  obogaćene  sa  5%  MZC  bili  su  delimično  zaštićeni  od  zračenja, shodno redukovanoj moždanoj aktivnosti SOD pri  2  Gy i sniženom nivou MDA pri izlaganju zračenju od 2 i  10 Gy.",
publisher = "Belgrade: Military Medical Academy, INI",
journal = "Vojnosanitetski pregled",
title = "Moderate radioprotective role of zeolite in rats, Umerena radioprotektivna uloga zeolita kod pacova",
volume = "78",
number = "7",
pages = "760-768",
doi = "10.2298/VSP190702136P"
}

Pavlović, M., Đukić, M., Vojvodić, D., Ninković, M., Stevanović, I., Đurić, A.,& Stanojević, B.. (2021). Moderate radioprotective role of zeolite in rats. in Vojnosanitetski pregled
Belgrade: Military Medical Academy, INI., 78(7), 760-768.
https://doi.org/10.2298/VSP190702136P

Pavlović M, Đukić M, Vojvodić D, Ninković M, Stevanović I, Đurić A, Stanojević B. Moderate radioprotective role of zeolite in rats. in Vojnosanitetski pregled. 2021;78(7):760-768.
doi:10.2298/VSP190702136P .

Pavlović, Miloš, Đukić, Mirjana, Vojvodić, Danilo, Ninković, Milica, Stevanović, Ivana, Đurić, Ana, Stanojević, Boban, "Moderate radioprotective role of zeolite in rats" in Vojnosanitetski pregled, 78, no. 7 (2021):760-768,
https://doi.org/10.2298/VSP190702136P . .

TY  - JOUR
AU  - Stevanović, Ivana
AU  - Ninković, Milica
AU  - Mančić, Bojana
AU  - Milivojević, Marija
AU  - Stojanović, Ivana
AU  - Ilić, Tihomir
AU  - Vujović, Maja
AU  - Đukić, Mirjana
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3658
AB  - Cortical theta burst stimulation (TBS) structured as intermittent (iTBS) and continuous (cTBS)could prevent the progression of the experimental autoimmune encephalomyelitis (EAE). The interplayof brain antioxidant defense systems against free radicals (FRs) overproduction induced by EAE,as well as during iTBS or cTBS, have not been entirely investigated.  This study aimed to examinewhether oxidative-nitrogen stress (ONS) is one of the underlying pathophysiological mechanisms ofEAE, which may be changed in terms of health improvement by iTBS or cTBS. Dark Agouti strainfemale rats were tested for the effects of EAE and TBS. The rats were randomly divided into the controlgroup, rats specifically immunized for EAE and nonspecifically immuno-stimulated with CompleteFreund’s adjuvant. TBS or sham TBS was applied to EAE rats from 14th–24th post-immunizationday. Superoxide dismutase activity, levels of superoxide anion (O2•–), lipid peroxidation, glutathione(GSH), nicotinamide adenine dinucleotide phosphate (NADPH), and thioredoxin reductase (TrxR)activity were analyzed in rat spinal cords homogenates. The severity of EAE clinical coincided withthe climax of ONS. The most critical result refers to TrxR, which immensely responded against theapplied stressors of the central nervous system (CNS), including immunization and TBS. We foundthat the compensatory neuroprotective role of TrxR upregulation is a positive feedback mechanismthat reduces the harmfulness of ONS. iTBS and cTBS both modulate the biochemical environmentagainst ONS at a distance from the area of stimulation, alleviating symptoms of EAE. The results ofour study increase the understanding of FRs’ interplay and the role of Trx/TrxR in ONS-associatedneuroinflammatory diseases, such as EAE. Also, our results might help the development of new ideasfor designing more effective medical treatment, combining neuropsychological with noninvasiveneurostimulation–neuromodulation techniques to patients living with MS.
PB  - MDPI
T2  - Molecules
T1  - Compensatory Neuroprotective Response of Thioredoxin Reductase against Oxidative-Nitrosative Stress Induced by Experimental Autoimmune Encephalomyelitis in Rats: Modulation by Theta Burst Stimulation
VL  - 25
IS  - 17
DO  - 10.3390/molecules25173922
ER  - 

@article{
author = "Stevanović, Ivana and Ninković, Milica and Mančić, Bojana and Milivojević, Marija and Stojanović, Ivana and Ilić, Tihomir and Vujović, Maja and Đukić, Mirjana",
year = "2020",
abstract = "Cortical theta burst stimulation (TBS) structured as intermittent (iTBS) and continuous (cTBS)could prevent the progression of the experimental autoimmune encephalomyelitis (EAE). The interplayof brain antioxidant defense systems against free radicals (FRs) overproduction induced by EAE,as well as during iTBS or cTBS, have not been entirely investigated.  This study aimed to examinewhether oxidative-nitrogen stress (ONS) is one of the underlying pathophysiological mechanisms ofEAE, which may be changed in terms of health improvement by iTBS or cTBS. Dark Agouti strainfemale rats were tested for the effects of EAE and TBS. The rats were randomly divided into the controlgroup, rats specifically immunized for EAE and nonspecifically immuno-stimulated with CompleteFreund’s adjuvant. TBS or sham TBS was applied to EAE rats from 14th–24th post-immunizationday. Superoxide dismutase activity, levels of superoxide anion (O2•–), lipid peroxidation, glutathione(GSH), nicotinamide adenine dinucleotide phosphate (NADPH), and thioredoxin reductase (TrxR)activity were analyzed in rat spinal cords homogenates. The severity of EAE clinical coincided withthe climax of ONS. The most critical result refers to TrxR, which immensely responded against theapplied stressors of the central nervous system (CNS), including immunization and TBS. We foundthat the compensatory neuroprotective role of TrxR upregulation is a positive feedback mechanismthat reduces the harmfulness of ONS. iTBS and cTBS both modulate the biochemical environmentagainst ONS at a distance from the area of stimulation, alleviating symptoms of EAE. The results ofour study increase the understanding of FRs’ interplay and the role of Trx/TrxR in ONS-associatedneuroinflammatory diseases, such as EAE. Also, our results might help the development of new ideasfor designing more effective medical treatment, combining neuropsychological with noninvasiveneurostimulation–neuromodulation techniques to patients living with MS.",
publisher = "MDPI",
journal = "Molecules",
title = "Compensatory Neuroprotective Response of Thioredoxin Reductase against Oxidative-Nitrosative Stress Induced by Experimental Autoimmune Encephalomyelitis in Rats: Modulation by Theta Burst Stimulation",
volume = "25",
number = "17",
doi = "10.3390/molecules25173922"
}

Stevanović, I., Ninković, M., Mančić, B., Milivojević, M., Stojanović, I., Ilić, T., Vujović, M.,& Đukić, M.. (2020). Compensatory Neuroprotective Response of Thioredoxin Reductase against Oxidative-Nitrosative Stress Induced by Experimental Autoimmune Encephalomyelitis in Rats: Modulation by Theta Burst Stimulation. in Molecules
MDPI., 25(17).
https://doi.org/10.3390/molecules25173922

Stevanović I, Ninković M, Mančić B, Milivojević M, Stojanović I, Ilić T, Vujović M, Đukić M. Compensatory Neuroprotective Response of Thioredoxin Reductase against Oxidative-Nitrosative Stress Induced by Experimental Autoimmune Encephalomyelitis in Rats: Modulation by Theta Burst Stimulation. in Molecules. 2020;25(17).
doi:10.3390/molecules25173922 .

Stevanović, Ivana, Ninković, Milica, Mančić, Bojana, Milivojević, Marija, Stojanović, Ivana, Ilić, Tihomir, Vujović, Maja, Đukić, Mirjana, "Compensatory Neuroprotective Response of Thioredoxin Reductase against Oxidative-Nitrosative Stress Induced by Experimental Autoimmune Encephalomyelitis in Rats: Modulation by Theta Burst Stimulation" in Molecules, 25, no. 17 (2020),
https://doi.org/10.3390/molecules25173922 . .

TY  - CONF
AU  - Pavlović, Dragana
AU  - Vukelić-Nikolić, Marija
AU  - Marčetić, Mirjana
AU  - Kitić, Dušanka
AU  - Kostić, Milica
AU  - Milutinović, Milica
AU  - Miladinović, Bojana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5305
AB  - Rubus idaeus L., folium (raspberry leaf) is according to EMA/HMPC
monograph indicated as traditional herbal medicinal product for: the symptomatic
relief of minor spasm associated with menstrual periods, the symptomatic treatment
of mild inflammation in the mouth or throat and the symptomatic treatment of mild
diarrhea. The aim of this work was quantitative determination of phenolic compounds
(namely: total polyphenols, tannins, flavonoids, arbutin and hydroquinone) and
antioxidant capacity of the different leaves extracts of wild and cultivated Rubus
ideus. Total polyphenols, tannins and flavonoids were determined
spectrophotometrically, HPLC method was employed for quantification of arbutin
and hydroquinone. DPPH test was used for fast antioxidant screening. Arbutin and
hydroquinone were absent in all samples despite some literature date on arbutin
presence in Rubus idaeus. Our phytochemical study showed that raspberry leaves
extracts contain significant amounts of polyphenolic compounds, which could be
useful in its quality estimation. Cultured raspberry leaf extracts seem to be reacher
source of this secondary metabolites while hydromethanolic extracts of wild growing
plant material exhibited the strongest antioxidant activity. Polyphenolic content and
demonstrated activity could, at least in part, explain pharmacological effects of
raspberry leaves.
PB  - Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš
PB  - Institute for Nature Conservation of Serbia
C3  - 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts
T1  - Assessment of polyphenol content and in vitro antioxidant potential of wild growing and cultured raspberry leaf
SP  - 178
EP  - 178
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5305
ER  - 

@conference{
author = "Pavlović, Dragana and Vukelić-Nikolić, Marija and Marčetić, Mirjana and Kitić, Dušanka and Kostić, Milica and Milutinović, Milica and Miladinović, Bojana",
year = "2019",
abstract = "Rubus idaeus L., folium (raspberry leaf) is according to EMA/HMPC
monograph indicated as traditional herbal medicinal product for: the symptomatic
relief of minor spasm associated with menstrual periods, the symptomatic treatment
of mild inflammation in the mouth or throat and the symptomatic treatment of mild
diarrhea. The aim of this work was quantitative determination of phenolic compounds
(namely: total polyphenols, tannins, flavonoids, arbutin and hydroquinone) and
antioxidant capacity of the different leaves extracts of wild and cultivated Rubus
ideus. Total polyphenols, tannins and flavonoids were determined
spectrophotometrically, HPLC method was employed for quantification of arbutin
and hydroquinone. DPPH test was used for fast antioxidant screening. Arbutin and
hydroquinone were absent in all samples despite some literature date on arbutin
presence in Rubus idaeus. Our phytochemical study showed that raspberry leaves
extracts contain significant amounts of polyphenolic compounds, which could be
useful in its quality estimation. Cultured raspberry leaf extracts seem to be reacher
source of this secondary metabolites while hydromethanolic extracts of wild growing
plant material exhibited the strongest antioxidant activity. Polyphenolic content and
demonstrated activity could, at least in part, explain pharmacological effects of
raspberry leaves.",
publisher = "Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, Institute for Nature Conservation of Serbia",
journal = "13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts",
title = "Assessment of polyphenol content and in vitro antioxidant potential of wild growing and cultured raspberry leaf",
pages = "178-178",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5305"
}

Pavlović, D., Vukelić-Nikolić, M., Marčetić, M., Kitić, D., Kostić, M., Milutinović, M.,& Miladinović, B.. (2019). Assessment of polyphenol content and in vitro antioxidant potential of wild growing and cultured raspberry leaf. in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts
Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš., 178-178.
https://hdl.handle.net/21.15107/rcub_farfar_5305

Pavlović D, Vukelić-Nikolić M, Marčetić M, Kitić D, Kostić M, Milutinović M, Miladinović B. Assessment of polyphenol content and in vitro antioxidant potential of wild growing and cultured raspberry leaf. in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts. 2019;:178-178.
https://hdl.handle.net/21.15107/rcub_farfar_5305 .

Pavlović, Dragana, Vukelić-Nikolić, Marija, Marčetić, Mirjana, Kitić, Dušanka, Kostić, Milica, Milutinović, Milica, Miladinović, Bojana, "Assessment of polyphenol content and in vitro antioxidant potential of wild growing and cultured raspberry leaf" in 13th Symposium on the Flora of Southeastern Serbia and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts (2019):178-178,
https://hdl.handle.net/21.15107/rcub_farfar_5305 .

TY  - JOUR
AU  - Đukić, Mirjana
AU  - Fesatidou, Mara
AU  - Xenikakis, Iakovos
AU  - Geronikaki, Athina
AU  - Angelova, Violina T.
AU  - Savić, Vladimir
AU  - Pasić, Marta
AU  - Krilović, Branislav
AU  - Đukić, Dušan
AU  - Gobeljić, Borko
AU  - Pavlica, Marina
AU  - Đurić, Ana
AU  - Stanojević, Ivan
AU  - Vojvodić, Danilo
AU  - Saso, Luciano
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3154
AB  - The initial steps in preclinical drug developing research concern the synthesis of new compounds for specific therapeutic use which needs to be confirmed by in vitro and then in vivo testing. Nine thiazolidinone derivatives (numerically labeled 1-9) classified as follows: 1,3-thiazole-based compounds (1 and 2); 1,3,4-thiadiazole based compounds (3 and 4); substituted 5-benzylideno-2-adamantylthiazol[3,2-b] [1,2,4] triazol-6(5H) ones (5-8); and an ethylaminothiazole-based chalcone (9), were tested for antioxidant activity (AOA) by using three in vitro assays: DPPH (1,1-diphenyl-2-picrylhydrazyl scavenging capacity test); FRAP (ferric reducing antioxidant power test); and TBARS (thiobarbituric acid reactive substances test). Compounds 1-4 and 9 in particular are newly synthesized compounds. Also, traditional antioxidants Vitamins E and C and alpha-lipoic acid (alpha-LA) were tested. The results of DPPH testing: Vitamin C 94.35%, Vitamin E 2.99% and alpha-LA 1.57%; compounds: 4 33.98%; 2 18.73%; 1 15.62%; 5 6.59%; 3 4.99%; 6-9 demonstrated almost no AOA. The results of TBARS testing (% of LPO inhibition): Vitamin C 62.32%; Vitamin E 36.29%; alpha-LA 51.36%; compounds: 1 62.11%; 5 66.71%; 9 60.93%; 4, 6 and 7 demonstrated similar to 50%; 3 and 8 displayed similar to 38%; 2 23.51%. By FRAP method, Vitamins E and C showed equal AOA, similar to 100%, unlike alpha-LA (no AOA), and AOA of the tested compounds (expressed as a fraction of the AOA of Vitamin C) were: 2 and 4-75%; 8, 3 and 1-45%; 5-7 and 9-27%. Different red-ox reaction principles between these assays dictate different AOA outcomes for a single compound. Vitamin C appeared to be the superior antioxidant out of the traditional antioxidants; and compound 4 was superior to other tested thiazolidinone derivatives. Vitamin C appeared to be the superior antioxidant out of the traditional antioxidants; and compound 4 was superior to other tested thiazolidinone derivatives. Phenyl-functionalized benzylidene, amino-carbonyl functional domains and chelating ligand properties of the thiazolidinone derivatives correlated with AOA.
PB  - Elsevier Ireland Ltd, Clare
T2  - Chemico-Biological Interactions
T1  - In vitro antioxidant activity of thiazolidinone derivatives of 1,3-thiazole and 1,3,4-thiadiazole
VL  - 286
SP  - 119
EP  - 131
DO  - 10.1016/j.cbi.2018.03.013
ER  - 

@article{
author = "Đukić, Mirjana and Fesatidou, Mara and Xenikakis, Iakovos and Geronikaki, Athina and Angelova, Violina T. and Savić, Vladimir and Pasić, Marta and Krilović, Branislav and Đukić, Dušan and Gobeljić, Borko and Pavlica, Marina and Đurić, Ana and Stanojević, Ivan and Vojvodić, Danilo and Saso, Luciano",
year = "2018",
abstract = "The initial steps in preclinical drug developing research concern the synthesis of new compounds for specific therapeutic use which needs to be confirmed by in vitro and then in vivo testing. Nine thiazolidinone derivatives (numerically labeled 1-9) classified as follows: 1,3-thiazole-based compounds (1 and 2); 1,3,4-thiadiazole based compounds (3 and 4); substituted 5-benzylideno-2-adamantylthiazol[3,2-b] [1,2,4] triazol-6(5H) ones (5-8); and an ethylaminothiazole-based chalcone (9), were tested for antioxidant activity (AOA) by using three in vitro assays: DPPH (1,1-diphenyl-2-picrylhydrazyl scavenging capacity test); FRAP (ferric reducing antioxidant power test); and TBARS (thiobarbituric acid reactive substances test). Compounds 1-4 and 9 in particular are newly synthesized compounds. Also, traditional antioxidants Vitamins E and C and alpha-lipoic acid (alpha-LA) were tested. The results of DPPH testing: Vitamin C 94.35%, Vitamin E 2.99% and alpha-LA 1.57%; compounds: 4 33.98%; 2 18.73%; 1 15.62%; 5 6.59%; 3 4.99%; 6-9 demonstrated almost no AOA. The results of TBARS testing (% of LPO inhibition): Vitamin C 62.32%; Vitamin E 36.29%; alpha-LA 51.36%; compounds: 1 62.11%; 5 66.71%; 9 60.93%; 4, 6 and 7 demonstrated similar to 50%; 3 and 8 displayed similar to 38%; 2 23.51%. By FRAP method, Vitamins E and C showed equal AOA, similar to 100%, unlike alpha-LA (no AOA), and AOA of the tested compounds (expressed as a fraction of the AOA of Vitamin C) were: 2 and 4-75%; 8, 3 and 1-45%; 5-7 and 9-27%. Different red-ox reaction principles between these assays dictate different AOA outcomes for a single compound. Vitamin C appeared to be the superior antioxidant out of the traditional antioxidants; and compound 4 was superior to other tested thiazolidinone derivatives. Vitamin C appeared to be the superior antioxidant out of the traditional antioxidants; and compound 4 was superior to other tested thiazolidinone derivatives. Phenyl-functionalized benzylidene, amino-carbonyl functional domains and chelating ligand properties of the thiazolidinone derivatives correlated with AOA.",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Chemico-Biological Interactions",
title = "In vitro antioxidant activity of thiazolidinone derivatives of 1,3-thiazole and 1,3,4-thiadiazole",
volume = "286",
pages = "119-131",
doi = "10.1016/j.cbi.2018.03.013"
}

Đukić, M., Fesatidou, M., Xenikakis, I., Geronikaki, A., Angelova, V. T., Savić, V., Pasić, M., Krilović, B., Đukić, D., Gobeljić, B., Pavlica, M., Đurić, A., Stanojević, I., Vojvodić, D.,& Saso, L.. (2018). In vitro antioxidant activity of thiazolidinone derivatives of 1,3-thiazole and 1,3,4-thiadiazole. in Chemico-Biological Interactions
Elsevier Ireland Ltd, Clare., 286, 119-131.
https://doi.org/10.1016/j.cbi.2018.03.013

Đukić M, Fesatidou M, Xenikakis I, Geronikaki A, Angelova VT, Savić V, Pasić M, Krilović B, Đukić D, Gobeljić B, Pavlica M, Đurić A, Stanojević I, Vojvodić D, Saso L. In vitro antioxidant activity of thiazolidinone derivatives of 1,3-thiazole and 1,3,4-thiadiazole. in Chemico-Biological Interactions. 2018;286:119-131.
doi:10.1016/j.cbi.2018.03.013 .

Đukić, Mirjana, Fesatidou, Mara, Xenikakis, Iakovos, Geronikaki, Athina, Angelova, Violina T., Savić, Vladimir, Pasić, Marta, Krilović, Branislav, Đukić, Dušan, Gobeljić, Borko, Pavlica, Marina, Đurić, Ana, Stanojević, Ivan, Vojvodić, Danilo, Saso, Luciano, "In vitro antioxidant activity of thiazolidinone derivatives of 1,3-thiazole and 1,3,4-thiadiazole" in Chemico-Biological Interactions, 286 (2018):119-131,
https://doi.org/10.1016/j.cbi.2018.03.013 . .

TY  - JOUR
AU  - Pavlović, Milan
AU  - Apostolović, Svetlana
AU  - Stokanović, Dragana
AU  - Lilić, Jelena
AU  - Konstantinović, Sandra S.
AU  - Zvezdanović, Jelena B.
AU  - Marinković, Valentina
AU  - Nikolić, Valentina N.
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3094
AB  - Background A significant number of ischemic events occur even when adhering to dual antiplatelet therapy including aspirin and clopidogrel. Objectives The aim of our study was to determine predictors of long-term patient clinical outcome, among variables such as prodrug clopidogrel and intermediary metabolite 2-oxoclopidogrel concentrations, as well as patients' clinical characteristics. Setting Department for the Treatment of Acute Coronary Syndrome in tertiary teaching hospital, Serbia. Methods This study enrolled 88 consecutive patients with first STEMI, treated with primary PCI, within 6h of the chest pain onsetand followed them 40 months. On the third day of hospitalization, blood samples were collected from each patient to measure clopidogrel and its metabolite 2-oxo-clopidogrel concentration by UHPLC-DAD-MS method. Main outcome measure Mortality from cardiovascular causes, nonfatal myocardial infarction, nonfatal stroke or hospitalization for urgent myocardial revascularization or heart failure. Results The composite clinical outcome of cardiovascular mortality, nonfatal myocardial infarction, nonfatal stroke, or hospitalization for urgent myocardial revascularization or heart failure, was registered in 31 patients (35.2%) during the 40-month follow-up. Lower clopidogrel (p lt 0.05) and dose-adjusted clopidogrel concentrations (p lt 0.05) were associated with the higher incidence of composite outcome events. Their low plasma concentrations may be predicted by fentanyl administration (p lt 0.001) and creatinine clearance (p lt 0.01). The decrease in dose-adjusted clopidogrel unit for each ng/ml/mg increases the risk 21.7 times (p lt 0.05). Conclusion Clopidogrel dose-adjusted plasma concentration in STEMI patients, as well as multivessel coronary artery disease, showed significance in predicting an unfavorable composite clinical outcome after 40-month follow-up.
PB  - Springer, Dordrecht
T2  - International Journal of Clinical Pharmacy
T1  - The association of clopidogrel and 2-oxo-clopidogrel plasma levels and the 40 months clinical outcome after primary PCI
VL  - 40
IS  - 6
SP  - 1482
EP  - 1489
DO  - 10.1007/s11096-018-0730-9
ER  - 

@article{
author = "Pavlović, Milan and Apostolović, Svetlana and Stokanović, Dragana and Lilić, Jelena and Konstantinović, Sandra S. and Zvezdanović, Jelena B. and Marinković, Valentina and Nikolić, Valentina N.",
year = "2018",
abstract = "Background A significant number of ischemic events occur even when adhering to dual antiplatelet therapy including aspirin and clopidogrel. Objectives The aim of our study was to determine predictors of long-term patient clinical outcome, among variables such as prodrug clopidogrel and intermediary metabolite 2-oxoclopidogrel concentrations, as well as patients' clinical characteristics. Setting Department for the Treatment of Acute Coronary Syndrome in tertiary teaching hospital, Serbia. Methods This study enrolled 88 consecutive patients with first STEMI, treated with primary PCI, within 6h of the chest pain onsetand followed them 40 months. On the third day of hospitalization, blood samples were collected from each patient to measure clopidogrel and its metabolite 2-oxo-clopidogrel concentration by UHPLC-DAD-MS method. Main outcome measure Mortality from cardiovascular causes, nonfatal myocardial infarction, nonfatal stroke or hospitalization for urgent myocardial revascularization or heart failure. Results The composite clinical outcome of cardiovascular mortality, nonfatal myocardial infarction, nonfatal stroke, or hospitalization for urgent myocardial revascularization or heart failure, was registered in 31 patients (35.2%) during the 40-month follow-up. Lower clopidogrel (p lt 0.05) and dose-adjusted clopidogrel concentrations (p lt 0.05) were associated with the higher incidence of composite outcome events. Their low plasma concentrations may be predicted by fentanyl administration (p lt 0.001) and creatinine clearance (p lt 0.01). The decrease in dose-adjusted clopidogrel unit for each ng/ml/mg increases the risk 21.7 times (p lt 0.05). Conclusion Clopidogrel dose-adjusted plasma concentration in STEMI patients, as well as multivessel coronary artery disease, showed significance in predicting an unfavorable composite clinical outcome after 40-month follow-up.",
publisher = "Springer, Dordrecht",
journal = "International Journal of Clinical Pharmacy",
title = "The association of clopidogrel and 2-oxo-clopidogrel plasma levels and the 40 months clinical outcome after primary PCI",
volume = "40",
number = "6",
pages = "1482-1489",
doi = "10.1007/s11096-018-0730-9"
}

Pavlović, M., Apostolović, S., Stokanović, D., Lilić, J., Konstantinović, S. S., Zvezdanović, J. B., Marinković, V.,& Nikolić, V. N.. (2018). The association of clopidogrel and 2-oxo-clopidogrel plasma levels and the 40 months clinical outcome after primary PCI. in International Journal of Clinical Pharmacy
Springer, Dordrecht., 40(6), 1482-1489.
https://doi.org/10.1007/s11096-018-0730-9

Pavlović M, Apostolović S, Stokanović D, Lilić J, Konstantinović SS, Zvezdanović JB, Marinković V, Nikolić VN. The association of clopidogrel and 2-oxo-clopidogrel plasma levels and the 40 months clinical outcome after primary PCI. in International Journal of Clinical Pharmacy. 2018;40(6):1482-1489.
doi:10.1007/s11096-018-0730-9 .

Pavlović, Milan, Apostolović, Svetlana, Stokanović, Dragana, Lilić, Jelena, Konstantinović, Sandra S., Zvezdanović, Jelena B., Marinković, Valentina, Nikolić, Valentina N., "The association of clopidogrel and 2-oxo-clopidogrel plasma levels and the 40 months clinical outcome after primary PCI" in International Journal of Clinical Pharmacy, 40, no. 6 (2018):1482-1489,
https://doi.org/10.1007/s11096-018-0730-9 . .

TY  - JOUR
AU  - Taso, Ervin
AU  - Stefanović, Vladimir
AU  - Stevanović, Ivana
AU  - Vojvodić, Danilo
AU  - Topić, Aleksandra
AU  - Petković-Ćurčin, Aleksandra
AU  - Obradović-Đuričić, Kosovka
AU  - Marković, Aleksa
AU  - Đukić, Mirjana
AU  - Vujanović, Dragana
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3219
AB  - Biocompatibility of dental materials (DM) can be evaluated by gingival crevicular fluid (GCF) oxidative stress (OS) status. The goal of the study was to ascertain influence of dental caries degree, teeth position, and type and amount of applied DM on GCF OS profile. For this purpose, we tested six DMs that were sealed in one session: amalgam (Amg), composites: Tetric EvoCeram and Beautifil (BF), phosphate cement-zinc phosphate and polycarboxylate cements zinc polycarboxylate cements, and glass ionomer cement (GIC). The study included 88 dental outpatients. Follow-up was scheduled at 7th and 30th day. Oxidative stress parameters (malondialdehyde (MDA) and glutathione (GSH) levels and total superoxide dismutase (tSOD) activity) were measured before (0th day) and after the treatment (7th and 30th day) in GCF. Control teeth were mirror-positioned healthy teeth. The DM accomplished the following effects (listed in descending order): increase of GSH in GCF was realized by ZPoC > BF > GIC > Amg; tSOD activity increase by ZPoC > BF > Amg; and MDA decrease by ZPoC > ZPhC > Amg > TEC. Dental caries provokes insignificant rise of OS in GCF. ZPoC and ZPhC showed the highest antioxidant effect, contrary to GIC. Restorations with antioxidant properties may reduce gum diseases initiated by caries lesion, what is of great clinical relevance in dentistry.
PB  - Hindawi Ltd, London
T2  - Oxidative Medicine and Cellular Longevity
T1  - Influence of Dental Restorations on Oxidative Stress in Gingival Crevicular Fluid
DO  - 10.1155/2018/1823189
ER  - 

@article{
author = "Taso, Ervin and Stefanović, Vladimir and Stevanović, Ivana and Vojvodić, Danilo and Topić, Aleksandra and Petković-Ćurčin, Aleksandra and Obradović-Đuričić, Kosovka and Marković, Aleksa and Đukić, Mirjana and Vujanović, Dragana",
year = "2018",
abstract = "Biocompatibility of dental materials (DM) can be evaluated by gingival crevicular fluid (GCF) oxidative stress (OS) status. The goal of the study was to ascertain influence of dental caries degree, teeth position, and type and amount of applied DM on GCF OS profile. For this purpose, we tested six DMs that were sealed in one session: amalgam (Amg), composites: Tetric EvoCeram and Beautifil (BF), phosphate cement-zinc phosphate and polycarboxylate cements zinc polycarboxylate cements, and glass ionomer cement (GIC). The study included 88 dental outpatients. Follow-up was scheduled at 7th and 30th day. Oxidative stress parameters (malondialdehyde (MDA) and glutathione (GSH) levels and total superoxide dismutase (tSOD) activity) were measured before (0th day) and after the treatment (7th and 30th day) in GCF. Control teeth were mirror-positioned healthy teeth. The DM accomplished the following effects (listed in descending order): increase of GSH in GCF was realized by ZPoC > BF > GIC > Amg; tSOD activity increase by ZPoC > BF > Amg; and MDA decrease by ZPoC > ZPhC > Amg > TEC. Dental caries provokes insignificant rise of OS in GCF. ZPoC and ZPhC showed the highest antioxidant effect, contrary to GIC. Restorations with antioxidant properties may reduce gum diseases initiated by caries lesion, what is of great clinical relevance in dentistry.",
publisher = "Hindawi Ltd, London",
journal = "Oxidative Medicine and Cellular Longevity",
title = "Influence of Dental Restorations on Oxidative Stress in Gingival Crevicular Fluid",
doi = "10.1155/2018/1823189"
}

Taso, E., Stefanović, V., Stevanović, I., Vojvodić, D., Topić, A., Petković-Ćurčin, A., Obradović-Đuričić, K., Marković, A., Đukić, M.,& Vujanović, D.. (2018). Influence of Dental Restorations on Oxidative Stress in Gingival Crevicular Fluid. in Oxidative Medicine and Cellular Longevity
Hindawi Ltd, London..
https://doi.org/10.1155/2018/1823189

Taso E, Stefanović V, Stevanović I, Vojvodić D, Topić A, Petković-Ćurčin A, Obradović-Đuričić K, Marković A, Đukić M, Vujanović D. Influence of Dental Restorations on Oxidative Stress in Gingival Crevicular Fluid. in Oxidative Medicine and Cellular Longevity. 2018;.
doi:10.1155/2018/1823189 .

Taso, Ervin, Stefanović, Vladimir, Stevanović, Ivana, Vojvodić, Danilo, Topić, Aleksandra, Petković-Ćurčin, Aleksandra, Obradović-Đuričić, Kosovka, Marković, Aleksa, Đukić, Mirjana, Vujanović, Dragana, "Influence of Dental Restorations on Oxidative Stress in Gingival Crevicular Fluid" in Oxidative Medicine and Cellular Longevity (2018),
https://doi.org/10.1155/2018/1823189 . .

TY  - THES
AU  - Đurić, Ana
PY  - 2017
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=5449
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:16836/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=49498383
UR  - http://nardus.mpn.gov.rs/123456789/8967
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3386
AB  - Dosadašnja istraživanja mehanizama toksičnosti alkohola i kadmijuma (Cd) pri pojedinačnoj i udruženoj izloženosti eksprimantalnih životinja potvrđuju da oba agensa dovode do reproduktivne toksičnosti. Brojne epidemiološke studije su takođe ukazale da su pušenje i alkoholizam uzročnici poremećaja u spermatogenezi i neplodnosti kod muškaraca. S obzirom na činjenicu da je duvanski dim značajan izvor izloženosti Cd, kao i da su alkoholičari uglavnom i pušači, potrebno je utvrditi kako disulfiram (DSF), lek koji se koristi u averzivnoj terapiji alkoholizma, utiče na reproduktivnu toksičnost izazvanu pomenutim toksikantima. Cilj ove studije je bio da se ispita efekat DSF na narušenu homeostazu bioelemenata, redoks i androgeni status u testisima pacova prethodno izloženih alkoholu i/ili Cd kao i tokom koekspozicije sa Cd. Slobodna sulfhidrilna grupa (–SH) u hemijskoj strukturi metabolita DSF, dietilditiokarbamata (DDTC), ukazuje na redukcioni i helirajući potencijal DSF koji umnogome može da promeni metabolizam kako ispitivanih toksičnih agenasa (alkohola i Cd), tako i esencijalnih bioelemenata, kao i endogenih jedinjenja koja poseduju –SH grupe zbog mogućih reakcija tiolnog ukrštanja.Studija na životinjama (mužjaci pacova Wistar soja, n = 288) je dizajnirana sa ciljem da se imitiraju navike alkoholičara-pušača pre i nakon uvođenja DSF. Životinje su bile izložene ispitivanim supstancama subakutno (alkoholu i DSF), odnosno subhronično (Cd). Alkohol, DSF i maslinovo ulje (MU) kao rastvarač za DSF, su davani putem oralne sonde (per os), a Cd intraperitonelano (i.p.), svakodnevno u jednoj dozi. Životinje su slučajnim odabirom podeljene na kontrolne grupe i 7 eksperimentalnih grupa, od kojih je svaka grupa podeljena u 4 ili 6 podgrupa (n = 6) u odnosu na dužinu izloženosti svakoj od ispitivanih supstanci pojedinačno ili njihovoj kombinovanoj primeni. Podela eksperimentalnih životinja na grupe u odnosu na trajanje ekspozicije (1, 3, 10, 21 i 42 dana) je sledeća: tri kontrolne grupe (K1-42 , MU1-21, i K1-21/MU22-42); tri grupe koje su individualno bile izložene ispitivanim agensima (A1-21, Cd1-42 i DSF1-21); i četiri grupe koje su bile paralelno izložene ispitivanim agesnima (A1-21/Cd1-21, A1-21/DSF22-42, Cd1-42/DSF22-42 i A1-21/Cd1-42/DSF22-42)...
AB  - Previous animal studies on the mechanisms of toxicity after exposure or co-exposure to alcohol and cadmium (Cd) confirmed that both agents induce reproductive toxicity. Numerous epidemiological studies have also suggested that smoking and alcoholism cause disturbances in spermatogenesis and infertility in men. Due to the fact that tobacco smoke is an important source of Cd exposure, as well as alcoholics are usually smokers too, it is necessary to determine if disulfiram (DSF), a drug used in aversive therapy of alcoholism, affects the reproductive toxicity induced by the abovementioned toxicants. The aim of this study was to examine the effect of DSF on disturbed bioelements’ homeostasis, redox and androgen status in the testes of rats previously exposed to alcohol and / or Cd and during co-exposure with Cd. Free sulfhydryl group (-SH) in the chemical structure of the DSF’s metabolite, diethyldithiocarbamate (DDTC), indicates the reducing and chelating potential of DSF, which in many ways can change the metabolism of tested toxic agents (A and Cd), as well as essential bioelements, and endogenous compounds containing -SH groups due to possible reactions of thiol linking.The animal study (male Wistar rats, n = 288) was designed in order to imitate the conditions of alcoholics-smokers before and after the introduction of DSF. The animals were exposed to the tested substances subacute (alcohol and DSF) and subchronic (Cd). Alcohol, DSF, and olive oil (OL), as a solvent for DSF, were administered via oral gavage (per os), and Cd intraperitoneally, in a single daily dose. Animals were randomly divided into control groups and 7 experimental groups, each group was divided into 4 or 6 subgroups (n = 6) according to the duration of exposure to the tested substances alone or in combination. The division of the groups of the experimental animals with respect to the exposure time (1, 3, 10, 21 and 42 days) was as follows: three control groups (C1-42, OL1-21 and C1-21 / OL22-42); three groups which were independently exposed to the tested agents (A1-21, Cd1-42 and DSF1-21); and four groups that were subjected parallelly to the tested agents (A1-21 / Cd1-21, A1-21 / DSF22-42, Cd1-42 / DSF22-42 and A1-21 / Cd1-42 / DSF22-42)...
PB  - Универзитет у Београду, Фармацеутски факултет
T2  - Универзитет у Београду
T1  - Efekat disulfirama na mehanizme reproduktivne subakutne toksičnosti etanola i/ili kadmijuma kod mužjaka pacova
UR  - https://hdl.handle.net/21.15107/rcub_nardus_8967
ER  - 

@phdthesis{
author = "Đurić, Ana",
year = "2017",
abstract = "Dosadašnja istraživanja mehanizama toksičnosti alkohola i kadmijuma (Cd) pri pojedinačnoj i udruženoj izloženosti eksprimantalnih životinja potvrđuju da oba agensa dovode do reproduktivne toksičnosti. Brojne epidemiološke studije su takođe ukazale da su pušenje i alkoholizam uzročnici poremećaja u spermatogenezi i neplodnosti kod muškaraca. S obzirom na činjenicu da je duvanski dim značajan izvor izloženosti Cd, kao i da su alkoholičari uglavnom i pušači, potrebno je utvrditi kako disulfiram (DSF), lek koji se koristi u averzivnoj terapiji alkoholizma, utiče na reproduktivnu toksičnost izazvanu pomenutim toksikantima. Cilj ove studije je bio da se ispita efekat DSF na narušenu homeostazu bioelemenata, redoks i androgeni status u testisima pacova prethodno izloženih alkoholu i/ili Cd kao i tokom koekspozicije sa Cd. Slobodna sulfhidrilna grupa (–SH) u hemijskoj strukturi metabolita DSF, dietilditiokarbamata (DDTC), ukazuje na redukcioni i helirajući potencijal DSF koji umnogome može da promeni metabolizam kako ispitivanih toksičnih agenasa (alkohola i Cd), tako i esencijalnih bioelemenata, kao i endogenih jedinjenja koja poseduju –SH grupe zbog mogućih reakcija tiolnog ukrštanja.Studija na životinjama (mužjaci pacova Wistar soja, n = 288) je dizajnirana sa ciljem da se imitiraju navike alkoholičara-pušača pre i nakon uvođenja DSF. Životinje su bile izložene ispitivanim supstancama subakutno (alkoholu i DSF), odnosno subhronično (Cd). Alkohol, DSF i maslinovo ulje (MU) kao rastvarač za DSF, su davani putem oralne sonde (per os), a Cd intraperitonelano (i.p.), svakodnevno u jednoj dozi. Životinje su slučajnim odabirom podeljene na kontrolne grupe i 7 eksperimentalnih grupa, od kojih je svaka grupa podeljena u 4 ili 6 podgrupa (n = 6) u odnosu na dužinu izloženosti svakoj od ispitivanih supstanci pojedinačno ili njihovoj kombinovanoj primeni. Podela eksperimentalnih životinja na grupe u odnosu na trajanje ekspozicije (1, 3, 10, 21 i 42 dana) je sledeća: tri kontrolne grupe (K1-42 , MU1-21, i K1-21/MU22-42); tri grupe koje su individualno bile izložene ispitivanim agensima (A1-21, Cd1-42 i DSF1-21); i četiri grupe koje su bile paralelno izložene ispitivanim agesnima (A1-21/Cd1-21, A1-21/DSF22-42, Cd1-42/DSF22-42 i A1-21/Cd1-42/DSF22-42)..., Previous animal studies on the mechanisms of toxicity after exposure or co-exposure to alcohol and cadmium (Cd) confirmed that both agents induce reproductive toxicity. Numerous epidemiological studies have also suggested that smoking and alcoholism cause disturbances in spermatogenesis and infertility in men. Due to the fact that tobacco smoke is an important source of Cd exposure, as well as alcoholics are usually smokers too, it is necessary to determine if disulfiram (DSF), a drug used in aversive therapy of alcoholism, affects the reproductive toxicity induced by the abovementioned toxicants. The aim of this study was to examine the effect of DSF on disturbed bioelements’ homeostasis, redox and androgen status in the testes of rats previously exposed to alcohol and / or Cd and during co-exposure with Cd. Free sulfhydryl group (-SH) in the chemical structure of the DSF’s metabolite, diethyldithiocarbamate (DDTC), indicates the reducing and chelating potential of DSF, which in many ways can change the metabolism of tested toxic agents (A and Cd), as well as essential bioelements, and endogenous compounds containing -SH groups due to possible reactions of thiol linking.The animal study (male Wistar rats, n = 288) was designed in order to imitate the conditions of alcoholics-smokers before and after the introduction of DSF. The animals were exposed to the tested substances subacute (alcohol and DSF) and subchronic (Cd). Alcohol, DSF, and olive oil (OL), as a solvent for DSF, were administered via oral gavage (per os), and Cd intraperitoneally, in a single daily dose. Animals were randomly divided into control groups and 7 experimental groups, each group was divided into 4 or 6 subgroups (n = 6) according to the duration of exposure to the tested substances alone or in combination. The division of the groups of the experimental animals with respect to the exposure time (1, 3, 10, 21 and 42 days) was as follows: three control groups (C1-42, OL1-21 and C1-21 / OL22-42); three groups which were independently exposed to the tested agents (A1-21, Cd1-42 and DSF1-21); and four groups that were subjected parallelly to the tested agents (A1-21 / Cd1-21, A1-21 / DSF22-42, Cd1-42 / DSF22-42 and A1-21 / Cd1-42 / DSF22-42)...",
publisher = "Универзитет у Београду, Фармацеутски факултет",
journal = "Универзитет у Београду",
title = "Efekat disulfirama na mehanizme reproduktivne subakutne toksičnosti etanola i/ili kadmijuma kod mužjaka pacova",
url = "https://hdl.handle.net/21.15107/rcub_nardus_8967"
}

Đurić, A.. (2017). Efekat disulfirama na mehanizme reproduktivne subakutne toksičnosti etanola i/ili kadmijuma kod mužjaka pacova. in Универзитет у Београду
Универзитет у Београду, Фармацеутски факултет..
https://hdl.handle.net/21.15107/rcub_nardus_8967

Đurić A. Efekat disulfirama na mehanizme reproduktivne subakutne toksičnosti etanola i/ili kadmijuma kod mužjaka pacova. in Универзитет у Београду. 2017;.
https://hdl.handle.net/21.15107/rcub_nardus_8967 .

Đurić, Ana, "Efekat disulfirama na mehanizme reproduktivne subakutne toksičnosti etanola i/ili kadmijuma kod mužjaka pacova" in Универзитет у Београду (2017),
https://hdl.handle.net/21.15107/rcub_nardus_8967 .

TY  - JOUR
AU  - Đurić, Ana
AU  - Begić, Aida
AU  - Gobeljić, Borko
AU  - Pantelić, Ana
AU  - Zebić, Goran
AU  - Stevanović, Ivana
AU  - Đurđević, Dragan
AU  - Ninković, Milica
AU  - Prokić, Vera
AU  - Stanojević, Ivan
AU  - Vojvodić, Danilo
AU  - Đukić, Mirjana
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2969
AB  - The aim of the study was to investigate if alcohol and disulfiram (DSF) individually and in combination affect bioelements' and red-ox homeostasis in testes of the exposed rats. The animals were divided into groups according to the duration of treatments (21 and/or 42 days): C-21/C-42 groups (controls); OL21 and OL22-42 groups (0.5 mL olive oil intake); A(1-21) groups (3 mL 20% ethanol intake); DSF1-21 groups (178.5 mg DSF/kg/day intake); and A(21)+DSF22-42 groups (the DSF ingestion followed previous 21 days' treatment with alcohol). The measured parameters in testes included metals: zinc (Zn), copper (Cu), iron (Fe), magnesium (Mg) and selenium (Se); as well as oxidative stress (OS) parameters: superoxide anion radical (O-2(center dot-)), glutathione reduced (GSH) and oxidized (GSSG), malondialdehyde (MDA), hydrogen peroxide (H2O2) decomposition and activities of total superoxide dismutase (tSOD), glutathione-Stransferase (GST) and glutathione reductase (GR). Metal status was changed in all experimental groups (Fe rose, Zn fell, while Cu increased in A(21)+DSF24-32 groups). Development of OS was demonstrated in A(1-21) groups, but not in DSF1-21 groups. In A(21)+DSF22-42 groups, OS was partially reduced compared to A groups (A(1-21)>MDA>C; A(1-21) lt GSH lt C). High metal-binding affinity of DSF/DDTC changes red-ox homeostasis in rat testes.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Food and Chemical Toxicology
T1  - Subacute alcohol and/or disulfiram intake affects bioelements and redox status in rat testes
VL  - 105
SP  - 44
EP  - 51
DO  - 10.1016/j.fct.2017.03.041
ER  - 

@article{
author = "Đurić, Ana and Begić, Aida and Gobeljić, Borko and Pantelić, Ana and Zebić, Goran and Stevanović, Ivana and Đurđević, Dragan and Ninković, Milica and Prokić, Vera and Stanojević, Ivan and Vojvodić, Danilo and Đukić, Mirjana",
year = "2017",
abstract = "The aim of the study was to investigate if alcohol and disulfiram (DSF) individually and in combination affect bioelements' and red-ox homeostasis in testes of the exposed rats. The animals were divided into groups according to the duration of treatments (21 and/or 42 days): C-21/C-42 groups (controls); OL21 and OL22-42 groups (0.5 mL olive oil intake); A(1-21) groups (3 mL 20% ethanol intake); DSF1-21 groups (178.5 mg DSF/kg/day intake); and A(21)+DSF22-42 groups (the DSF ingestion followed previous 21 days' treatment with alcohol). The measured parameters in testes included metals: zinc (Zn), copper (Cu), iron (Fe), magnesium (Mg) and selenium (Se); as well as oxidative stress (OS) parameters: superoxide anion radical (O-2(center dot-)), glutathione reduced (GSH) and oxidized (GSSG), malondialdehyde (MDA), hydrogen peroxide (H2O2) decomposition and activities of total superoxide dismutase (tSOD), glutathione-Stransferase (GST) and glutathione reductase (GR). Metal status was changed in all experimental groups (Fe rose, Zn fell, while Cu increased in A(21)+DSF24-32 groups). Development of OS was demonstrated in A(1-21) groups, but not in DSF1-21 groups. In A(21)+DSF22-42 groups, OS was partially reduced compared to A groups (A(1-21)>MDA>C; A(1-21) lt GSH lt C). High metal-binding affinity of DSF/DDTC changes red-ox homeostasis in rat testes.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Food and Chemical Toxicology",
title = "Subacute alcohol and/or disulfiram intake affects bioelements and redox status in rat testes",
volume = "105",
pages = "44-51",
doi = "10.1016/j.fct.2017.03.041"
}

Đurić, A., Begić, A., Gobeljić, B., Pantelić, A., Zebić, G., Stevanović, I., Đurđević, D., Ninković, M., Prokić, V., Stanojević, I., Vojvodić, D.,& Đukić, M.. (2017). Subacute alcohol and/or disulfiram intake affects bioelements and redox status in rat testes. in Food and Chemical Toxicology
Pergamon-Elsevier Science Ltd, Oxford., 105, 44-51.
https://doi.org/10.1016/j.fct.2017.03.041

Đurić A, Begić A, Gobeljić B, Pantelić A, Zebić G, Stevanović I, Đurđević D, Ninković M, Prokić V, Stanojević I, Vojvodić D, Đukić M. Subacute alcohol and/or disulfiram intake affects bioelements and redox status in rat testes. in Food and Chemical Toxicology. 2017;105:44-51.
doi:10.1016/j.fct.2017.03.041 .

Đurić, Ana, Begić, Aida, Gobeljić, Borko, Pantelić, Ana, Zebić, Goran, Stevanović, Ivana, Đurđević, Dragan, Ninković, Milica, Prokić, Vera, Stanojević, Ivan, Vojvodić, Danilo, Đukić, Mirjana, "Subacute alcohol and/or disulfiram intake affects bioelements and redox status in rat testes" in Food and Chemical Toxicology, 105 (2017):44-51,
https://doi.org/10.1016/j.fct.2017.03.041 . .

TY  - JOUR
AU  - Begić, Aida
AU  - Đurić, Ana
AU  - Ninković, Milica
AU  - Stevanović, Ivana
AU  - Đurđević, Dragan
AU  - Pavlović, Miloš
AU  - Jelić, Katarina
AU  - Pantelić, Ana
AU  - Zebić, Goran
AU  - Dejanović, Bratislav
AU  - Stanojević, Ivan
AU  - Vojvodić, Danilo
AU  - Milosavljević, Petar
AU  - Đukić, Mirjana
AU  - Saso, Luciano
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2963
AB  - Examination of cadmium (Cd) toxicity and disulfiram (DSF) effect on liver was focused on oxidative stress (OS), bioelements status, morphological and functional changes. Male Wistar rats were intraperitoneally treated with 1mg CdCl2/kg BW/day; orally with 178.5 mg DSF/kg BW/day for 1, 3, 10 and 21 days; and co-exposed from 22nd to 42nd day. The co-exposure nearly restored previously suppressed total superoxide dismutase (SOD), catalase (CAT) and increased glutathione peroxidase (GPx) activities; increased previously reduced glutathione reductase (GR) and total glutathione-S-transferase (GST) activities; reduced previously increased superoxide anion radical (O-2(center dot-)) and malondialdehyde (MDA) levels; increased zinc (Zn) and iron (Fe), and decreased copper (Cu) (yet above control value), while magnesium (Mg) was not affected; and decreased serum alanine aminotransferases (ALT) levels. Histopathological examination showed signs of inflammation process as previously demonstrated by exposure to Cd. Overall, we ascertained partial liver redox status improvement, compared with the formerly Cd-induced impact.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Journal of Enzyme Inhibition and Medicinal Chemistry
T1  - Disulfiram moderately restores impaired hepatic redox status of rats subchronically exposed to cadmium
VL  - 32
IS  - 1
SP  - 478
EP  - 489
DO  - 10.1080/14756366.2016.1261132
ER  - 

@article{
author = "Begić, Aida and Đurić, Ana and Ninković, Milica and Stevanović, Ivana and Đurđević, Dragan and Pavlović, Miloš and Jelić, Katarina and Pantelić, Ana and Zebić, Goran and Dejanović, Bratislav and Stanojević, Ivan and Vojvodić, Danilo and Milosavljević, Petar and Đukić, Mirjana and Saso, Luciano",
year = "2017",
abstract = "Examination of cadmium (Cd) toxicity and disulfiram (DSF) effect on liver was focused on oxidative stress (OS), bioelements status, morphological and functional changes. Male Wistar rats were intraperitoneally treated with 1mg CdCl2/kg BW/day; orally with 178.5 mg DSF/kg BW/day for 1, 3, 10 and 21 days; and co-exposed from 22nd to 42nd day. The co-exposure nearly restored previously suppressed total superoxide dismutase (SOD), catalase (CAT) and increased glutathione peroxidase (GPx) activities; increased previously reduced glutathione reductase (GR) and total glutathione-S-transferase (GST) activities; reduced previously increased superoxide anion radical (O-2(center dot-)) and malondialdehyde (MDA) levels; increased zinc (Zn) and iron (Fe), and decreased copper (Cu) (yet above control value), while magnesium (Mg) was not affected; and decreased serum alanine aminotransferases (ALT) levels. Histopathological examination showed signs of inflammation process as previously demonstrated by exposure to Cd. Overall, we ascertained partial liver redox status improvement, compared with the formerly Cd-induced impact.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Journal of Enzyme Inhibition and Medicinal Chemistry",
title = "Disulfiram moderately restores impaired hepatic redox status of rats subchronically exposed to cadmium",
volume = "32",
number = "1",
pages = "478-489",
doi = "10.1080/14756366.2016.1261132"
}

Begić, A., Đurić, A., Ninković, M., Stevanović, I., Đurđević, D., Pavlović, M., Jelić, K., Pantelić, A., Zebić, G., Dejanović, B., Stanojević, I., Vojvodić, D., Milosavljević, P., Đukić, M.,& Saso, L.. (2017). Disulfiram moderately restores impaired hepatic redox status of rats subchronically exposed to cadmium. in Journal of Enzyme Inhibition and Medicinal Chemistry
Taylor & Francis Ltd, Abingdon., 32(1), 478-489.
https://doi.org/10.1080/14756366.2016.1261132

Begić A, Đurić A, Ninković M, Stevanović I, Đurđević D, Pavlović M, Jelić K, Pantelić A, Zebić G, Dejanović B, Stanojević I, Vojvodić D, Milosavljević P, Đukić M, Saso L. Disulfiram moderately restores impaired hepatic redox status of rats subchronically exposed to cadmium. in Journal of Enzyme Inhibition and Medicinal Chemistry. 2017;32(1):478-489.
doi:10.1080/14756366.2016.1261132 .

Begić, Aida, Đurić, Ana, Ninković, Milica, Stevanović, Ivana, Đurđević, Dragan, Pavlović, Miloš, Jelić, Katarina, Pantelić, Ana, Zebić, Goran, Dejanović, Bratislav, Stanojević, Ivan, Vojvodić, Danilo, Milosavljević, Petar, Đukić, Mirjana, Saso, Luciano, "Disulfiram moderately restores impaired hepatic redox status of rats subchronically exposed to cadmium" in Journal of Enzyme Inhibition and Medicinal Chemistry, 32, no. 1 (2017):478-489,
https://doi.org/10.1080/14756366.2016.1261132 . .

TY  - JOUR
AU  - Begić, Aida
AU  - Đurić, Ana
AU  - Gobeljić, Borko
AU  - Stevanović, Ivana
AU  - Lukić, Vera
AU  - Stanojević, Ivan
AU  - Ninković, Milica
AU  - Saso, Luciano
AU  - Vojvodić, Danilo
AU  - Đukić, Mirjana
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2952
AB  - The aim of our work was to optimize and apply simple high-performance liquid chromatography method with ultraviolet detection (HPLC-UV) for simultaneous determination of reduced (GSH) and oxidized (GSSG) glutathione in biological matrix (specifically, the rat liver tissue was used herein), since the ratio between oxidized and reduced glutathione forms (GSSG-GSH) has been recognized as an important biological marker of oxidatively depleted GSH in oxidative stress (OS)-associated diseases and poisonings. An isocratic chromatographic separation of GSH and GSSG (2.8 min and 6.3 min, respectively) was performed with the mobile phase consisted of sodium perchlorate solution (pH adjusted to 2.8) at flow rate of 1 mL min(-1), detection set at 215 nm, and column temperature of 40 degrees C. The method offers short run time, linearity in the range of 0.01-200 mu M concentration for both compounds (R-2 = 1), low limits of detection and quantification (GSH: 0.18 mu M and 0.56 mu M, GSSG: 0.52 mu M and 1.58 mu M, respectively), precision, accuracy (bias  lt 2%), and high reproducibility. Through suitable sample handling, an overestimation of GSSG was prevented. High recovery (>99%) was achieved. The method was successfully applied for the analysis of GSH and GSSG in liver homogenates of Wistar rats intraperitoneally exposed to cadmium (Cd) (1 mg kg(-1) CdCl2/21 days). Regardless of other Cd-mediated hepatotoxicity mechanisms, herein, we have exclusively interpreted/emphasized oxidative GSH depletion. The presented method is acceptable for a routine analysis of GSH and GSSG in biological matrix, while the calculated ratio GSSG-GSH is considered as a valuable OS marker.
PB  - Akademiai Kiado Rt, Budapest
T2  - Acta Chromatographica
T1  - The Simple Isocratic HPLC-UV Method for the Simultaneous Determination of Reduced and Oxidized Glutathione in Animal Tissue
VL  - 29
IS  - 1
SP  - 67
EP  - 84
DO  - 10.1556/1326.2017.29.1.5
ER  - 

@article{
author = "Begić, Aida and Đurić, Ana and Gobeljić, Borko and Stevanović, Ivana and Lukić, Vera and Stanojević, Ivan and Ninković, Milica and Saso, Luciano and Vojvodić, Danilo and Đukić, Mirjana",
year = "2017",
abstract = "The aim of our work was to optimize and apply simple high-performance liquid chromatography method with ultraviolet detection (HPLC-UV) for simultaneous determination of reduced (GSH) and oxidized (GSSG) glutathione in biological matrix (specifically, the rat liver tissue was used herein), since the ratio between oxidized and reduced glutathione forms (GSSG-GSH) has been recognized as an important biological marker of oxidatively depleted GSH in oxidative stress (OS)-associated diseases and poisonings. An isocratic chromatographic separation of GSH and GSSG (2.8 min and 6.3 min, respectively) was performed with the mobile phase consisted of sodium perchlorate solution (pH adjusted to 2.8) at flow rate of 1 mL min(-1), detection set at 215 nm, and column temperature of 40 degrees C. The method offers short run time, linearity in the range of 0.01-200 mu M concentration for both compounds (R-2 = 1), low limits of detection and quantification (GSH: 0.18 mu M and 0.56 mu M, GSSG: 0.52 mu M and 1.58 mu M, respectively), precision, accuracy (bias  lt 2%), and high reproducibility. Through suitable sample handling, an overestimation of GSSG was prevented. High recovery (>99%) was achieved. The method was successfully applied for the analysis of GSH and GSSG in liver homogenates of Wistar rats intraperitoneally exposed to cadmium (Cd) (1 mg kg(-1) CdCl2/21 days). Regardless of other Cd-mediated hepatotoxicity mechanisms, herein, we have exclusively interpreted/emphasized oxidative GSH depletion. The presented method is acceptable for a routine analysis of GSH and GSSG in biological matrix, while the calculated ratio GSSG-GSH is considered as a valuable OS marker.",
publisher = "Akademiai Kiado Rt, Budapest",
journal = "Acta Chromatographica",
title = "The Simple Isocratic HPLC-UV Method for the Simultaneous Determination of Reduced and Oxidized Glutathione in Animal Tissue",
volume = "29",
number = "1",
pages = "67-84",
doi = "10.1556/1326.2017.29.1.5"
}

Begić, A., Đurić, A., Gobeljić, B., Stevanović, I., Lukić, V., Stanojević, I., Ninković, M., Saso, L., Vojvodić, D.,& Đukić, M.. (2017). The Simple Isocratic HPLC-UV Method for the Simultaneous Determination of Reduced and Oxidized Glutathione in Animal Tissue. in Acta Chromatographica
Akademiai Kiado Rt, Budapest., 29(1), 67-84.
https://doi.org/10.1556/1326.2017.29.1.5

Begić A, Đurić A, Gobeljić B, Stevanović I, Lukić V, Stanojević I, Ninković M, Saso L, Vojvodić D, Đukić M. The Simple Isocratic HPLC-UV Method for the Simultaneous Determination of Reduced and Oxidized Glutathione in Animal Tissue. in Acta Chromatographica. 2017;29(1):67-84.
doi:10.1556/1326.2017.29.1.5 .

Begić, Aida, Đurić, Ana, Gobeljić, Borko, Stevanović, Ivana, Lukić, Vera, Stanojević, Ivan, Ninković, Milica, Saso, Luciano, Vojvodić, Danilo, Đukić, Mirjana, "The Simple Isocratic HPLC-UV Method for the Simultaneous Determination of Reduced and Oxidized Glutathione in Animal Tissue" in Acta Chromatographica, 29, no. 1 (2017):67-84,
https://doi.org/10.1556/1326.2017.29.1.5 . .

TY  - JOUR
AU  - Pavlović, Dragana R.
AU  - Veljković, Milica
AU  - Stojanović, Nikola M.
AU  - Gocmanac-Ignjatović, Marija
AU  - Mihailov-Krstev, Tatjana
AU  - Branković, Suzana
AU  - Sokolović, Dušan
AU  - Marčetić, Mirjana
AU  - Radulović, Niko
AU  - Radenković, Mirjana
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2802
AB  - ObjectivesAs there are no previous studies of the European wild-garlic (Allium ursinum) effects on the gastrointestinal system, despite its traditional applications in gastrointestinal disorders' treatment and regular use in the human diet, we have quantified and compared spasmolytic, antimicrobial and antioxidant activities of its different leaf extracts. MethodsWild-garlic extracts were tested for spasmolytic activity on isolated rat ileum, antimicrobial activity on selected Gram-positive and Gram-negative bacteria and fungi by microdilution method and antioxidant capacity by DPPH radical-scavenging assay. Key findingsWild-garlic extracts were found to decrease ileal basal tone. As the relaxation of K+-induced contractions was similar to one caused by papaverin, the observed spasmolytic effect was most likely mediated through Ca2+-channel inhibition. Ethanolic extract (with the highest phenolic and high alk(en)yl cysteine sulphoxides' levels) produced the strongest spasmolytic activity. In case of acetylcholine-induced contractions, only hydromethanolic extract showed no statistical difference in comparison with positive control. All samples exhibited certain antioxidant potential and strong antimicrobial activity against tested enteropathogenic strains (Salmonella enteritidis was the most sensitive, followed by Escherichia coli, Proteus mirabilis and Enterococcus faecalis). ConclusionBesides other already established health-promoting effects, wild garlic could be useful in treatment of mild gastrointestinal disturbances.
PB  - Wiley, Hoboken
T2  - Journal of Pharmacy and Pharmacology
T1  - Influence of different wild-garlic (Allium ursinum) extracts on the gastrointestinal system: spasmolytic, antimicrobial and antioxidant properties
VL  - 69
IS  - 9
SP  - 1208
EP  - 1218
DO  - 10.1111/jphp.12746
ER  - 

@article{
author = "Pavlović, Dragana R. and Veljković, Milica and Stojanović, Nikola M. and Gocmanac-Ignjatović, Marija and Mihailov-Krstev, Tatjana and Branković, Suzana and Sokolović, Dušan and Marčetić, Mirjana and Radulović, Niko and Radenković, Mirjana",
year = "2017",
abstract = "ObjectivesAs there are no previous studies of the European wild-garlic (Allium ursinum) effects on the gastrointestinal system, despite its traditional applications in gastrointestinal disorders' treatment and regular use in the human diet, we have quantified and compared spasmolytic, antimicrobial and antioxidant activities of its different leaf extracts. MethodsWild-garlic extracts were tested for spasmolytic activity on isolated rat ileum, antimicrobial activity on selected Gram-positive and Gram-negative bacteria and fungi by microdilution method and antioxidant capacity by DPPH radical-scavenging assay. Key findingsWild-garlic extracts were found to decrease ileal basal tone. As the relaxation of K+-induced contractions was similar to one caused by papaverin, the observed spasmolytic effect was most likely mediated through Ca2+-channel inhibition. Ethanolic extract (with the highest phenolic and high alk(en)yl cysteine sulphoxides' levels) produced the strongest spasmolytic activity. In case of acetylcholine-induced contractions, only hydromethanolic extract showed no statistical difference in comparison with positive control. All samples exhibited certain antioxidant potential and strong antimicrobial activity against tested enteropathogenic strains (Salmonella enteritidis was the most sensitive, followed by Escherichia coli, Proteus mirabilis and Enterococcus faecalis). ConclusionBesides other already established health-promoting effects, wild garlic could be useful in treatment of mild gastrointestinal disturbances.",
publisher = "Wiley, Hoboken",
journal = "Journal of Pharmacy and Pharmacology",
title = "Influence of different wild-garlic (Allium ursinum) extracts on the gastrointestinal system: spasmolytic, antimicrobial and antioxidant properties",
volume = "69",
number = "9",
pages = "1208-1218",
doi = "10.1111/jphp.12746"
}

Pavlović, D. R., Veljković, M., Stojanović, N. M., Gocmanac-Ignjatović, M., Mihailov-Krstev, T., Branković, S., Sokolović, D., Marčetić, M., Radulović, N.,& Radenković, M.. (2017). Influence of different wild-garlic (Allium ursinum) extracts on the gastrointestinal system: spasmolytic, antimicrobial and antioxidant properties. in Journal of Pharmacy and Pharmacology
Wiley, Hoboken., 69(9), 1208-1218.
https://doi.org/10.1111/jphp.12746

Pavlović DR, Veljković M, Stojanović NM, Gocmanac-Ignjatović M, Mihailov-Krstev T, Branković S, Sokolović D, Marčetić M, Radulović N, Radenković M. Influence of different wild-garlic (Allium ursinum) extracts on the gastrointestinal system: spasmolytic, antimicrobial and antioxidant properties. in Journal of Pharmacy and Pharmacology. 2017;69(9):1208-1218.
doi:10.1111/jphp.12746 .

Pavlović, Dragana R., Veljković, Milica, Stojanović, Nikola M., Gocmanac-Ignjatović, Marija, Mihailov-Krstev, Tatjana, Branković, Suzana, Sokolović, Dušan, Marčetić, Mirjana, Radulović, Niko, Radenković, Mirjana, "Influence of different wild-garlic (Allium ursinum) extracts on the gastrointestinal system: spasmolytic, antimicrobial and antioxidant properties" in Journal of Pharmacy and Pharmacology, 69, no. 9 (2017):1208-1218,
https://doi.org/10.1111/jphp.12746 . .

TY  - JOUR
AU  - Stefanović, Vladimir
AU  - Taso, Ervin
AU  - Petković-Ćurčin, Aleksandra
AU  - Đukić, Mirjana
AU  - Gardašević, Milka
AU  - Rakić, Mia
AU  - Xavier, Struillou
AU  - Jović, Milena
AU  - Miller, Karolina
AU  - Stanojević, Ivan
AU  - Vojvodić, Danilo
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2668
AB  - Background/Aim. Several cytokines and lymphokines (IL1β, ENA78, IL6, TNFα, IL8 and S100A8) are expressed during dental pulp inflammation. Analysis of gingival crevicular fluid (GCF) offers a non-invasive means of studying general host response in oral cavity. Although GCF levels of various mediators could reflect the state of inflammation both in dental pulp and gingiva adjacent to a tooth, GCF samples of those without significant gingivitis could be interpreted as reflection of pulpal process. The aim of this study was to investigate IL9 GCF values in patients with dental caries and to assess possible influence of various dental fillings materials on local IL9 production. Methods. The study group included 90 patients, aged 18-70, with inclusion and exclusion criteria in the prospective clinical study. Of the 6 types of material used for the restoration of prepared cavities, 3 were intended for temporary and 3 for definitive restoration. According to dental fillings weight, all the participants were divided into 3 groups: those with fillings lighter than 0.50 g, those with 0.50-1.00 g, and those with fillings heavier than 1.00 g. Samples were taken from gingival sulcus using the filter paper technique. Clinical parameters were determined by bleeding index, plaque index (Silness-Lou, 0-3), gingival index (0-3), and gingival sulcus depth. Cytokine concentrations were assessed using commercially available cytomix. Results. According to the weight of dental fillings, there was a clear decreament trend of IL9 values meaning that dental defects greater than 1.00 g of dental filling were associated with lower GCF IL9 concentration. The IL9 values correlated with the degree of gingival index and depth of gingival sulcus, being higher with more advanced gingivitis and more pronounced anatomical changes in the tooth edge. Different filling materials exerted various local IL9 responses. Zink polycarbonate cement and amalgam fillings induced a significant and long-lasting local IL9 decrement, while the use of Tetric EvoCeram and GMA-BISK significantly increased IL9 levels. Conclusion. The obtained results indicate that IL9 GCF could be regarded as a measure of odontoblasts' response to the extensity of dental caries. The type of material used for dental fillings could profoundly alter biological function of gingival and pulpal cells. Also, the results obtained in this study suggest that some materials could even enhance wound repair by modulating macrophage activation.
AB  - Uvod/Cilj. Jedan broj citokina i limfokina (IL1β, ENA78, IL6, TNFα, IL8 I S100A8) izlučuje se tokom upale zubne pulpe. Analiza gingivalne sulkusne tečnosti (gingival crevicular fluid - GCF) omogućava neinvazivno proučavanje opšteg odgovora pacijenta na lokalne promene u usnoj duplji. Iako nivoi GCF mogu da odražavaju stanje upale kako u zubnoj pulpi, tako i u gingivi susednog zuba, uzorci GCF zuba bez značajnijeg gingivitisa mogli bi se tumačiti kao pokazatelji procesa u pulpi. Cilj ovog istraživanja bio je da se ispitaju nivoi IL9 u GCF kod pacijenata sa zubnim karijesom i da se utvrdi eventualni uticaj različitih materijala za zubne ispune na lokalnu proizvodnju IL9. Metode. U studiju je bilo uključeno 90 pacijenata, starih od 18 do 70 godina, uz primenu kriterijuma za uključivanje/isključivanje za prospektivne kliničke studije. Od ukupno šest materijala za punjenje pripremljenih kaviteta, tri je korišćeno za privremenu i tri za trajnu ispunu. Prema težini zubnog ispuna, pacijenti su bili podeljeni na tri grupe: oni sa ispunama lakšim od 0,50 g, sa ispunama od 0,50 do 1,00 g i sa ispunama težim od 1,00 g. Uzorci su uzimani iz gingivalnog sulkusa primenom tehnike filter papira. Korišćeni klinički parametri bili su indeks krvarenja, plak indeks (Silness-Lou, 0-3), gingivalni indeks (0-3) i dubina gingivalnog sulkusa. Koncentracije citokina određene su komercijalnim citomiksom. Rezultati. Težina zubne ispune ukazivala je na tendenciju opadanja vrednosti IL9, što je značilo da je veće oštećenje zuba, sa zubnom ispunom težom od 1,00 g, praćeno nižom koncentracijom IL9 u GCF. Vrednosti IL9 bile su u korelaciji sa stepenom gingivalnog indeksa i dubinom gingivalnog sulkusa, pogotovu u poodmaklom gingivitisu i izraženijim anatomskim promenama ivica zuba. Različite ispune izazivale su različite lokalne sekrecije IL9. Cink-polikarbonatni cement i amalgamska ispuna izazvale su značajan i dugotrajan pad nivoa lokalnog IL9, dok je primena tetrik-evocerama i GMA-BISK znatno povisila nivoe IL9. Zaključak. Rezultati dobijeni u ovoj studiji ukazuju da se IL9 u GCF može koristiti kao mera za reakciju odontoblasta na veličinu karijesa. Tip zubne ispune može da promeni biološku funkciju ćelija gingive i pulpe. Rezultati ove studije, takođe, ukazuju i na to da neke vrste ispuna mogu čak da ubrzaju zarastanje rane modulacijom aktivnosti makrofaga.
PB  - Vojnomedicinska akademija - Institut za naučne informacije, Beograd
T2  - Vojnosanitetski pregled
T1  - Influence of dental filling material type on the concentration of interleukin 9 in the samples of gingival crevicular fluid
T1  - Uticaj tipa materijala za plombiranje zuba na koncentraciju interleukina 9 u uzorcima gingivalne sulkusne tečnosti
VL  - 73
IS  - 8
SP  - 728
EP  - 734
DO  - 10.2298/VSP140227054S
ER  - 

@article{
author = "Stefanović, Vladimir and Taso, Ervin and Petković-Ćurčin, Aleksandra and Đukić, Mirjana and Gardašević, Milka and Rakić, Mia and Xavier, Struillou and Jović, Milena and Miller, Karolina and Stanojević, Ivan and Vojvodić, Danilo",
year = "2016",
abstract = "Background/Aim. Several cytokines and lymphokines (IL1β, ENA78, IL6, TNFα, IL8 and S100A8) are expressed during dental pulp inflammation. Analysis of gingival crevicular fluid (GCF) offers a non-invasive means of studying general host response in oral cavity. Although GCF levels of various mediators could reflect the state of inflammation both in dental pulp and gingiva adjacent to a tooth, GCF samples of those without significant gingivitis could be interpreted as reflection of pulpal process. The aim of this study was to investigate IL9 GCF values in patients with dental caries and to assess possible influence of various dental fillings materials on local IL9 production. Methods. The study group included 90 patients, aged 18-70, with inclusion and exclusion criteria in the prospective clinical study. Of the 6 types of material used for the restoration of prepared cavities, 3 were intended for temporary and 3 for definitive restoration. According to dental fillings weight, all the participants were divided into 3 groups: those with fillings lighter than 0.50 g, those with 0.50-1.00 g, and those with fillings heavier than 1.00 g. Samples were taken from gingival sulcus using the filter paper technique. Clinical parameters were determined by bleeding index, plaque index (Silness-Lou, 0-3), gingival index (0-3), and gingival sulcus depth. Cytokine concentrations were assessed using commercially available cytomix. Results. According to the weight of dental fillings, there was a clear decreament trend of IL9 values meaning that dental defects greater than 1.00 g of dental filling were associated with lower GCF IL9 concentration. The IL9 values correlated with the degree of gingival index and depth of gingival sulcus, being higher with more advanced gingivitis and more pronounced anatomical changes in the tooth edge. Different filling materials exerted various local IL9 responses. Zink polycarbonate cement and amalgam fillings induced a significant and long-lasting local IL9 decrement, while the use of Tetric EvoCeram and GMA-BISK significantly increased IL9 levels. Conclusion. The obtained results indicate that IL9 GCF could be regarded as a measure of odontoblasts' response to the extensity of dental caries. The type of material used for dental fillings could profoundly alter biological function of gingival and pulpal cells. Also, the results obtained in this study suggest that some materials could even enhance wound repair by modulating macrophage activation., Uvod/Cilj. Jedan broj citokina i limfokina (IL1β, ENA78, IL6, TNFα, IL8 I S100A8) izlučuje se tokom upale zubne pulpe. Analiza gingivalne sulkusne tečnosti (gingival crevicular fluid - GCF) omogućava neinvazivno proučavanje opšteg odgovora pacijenta na lokalne promene u usnoj duplji. Iako nivoi GCF mogu da odražavaju stanje upale kako u zubnoj pulpi, tako i u gingivi susednog zuba, uzorci GCF zuba bez značajnijeg gingivitisa mogli bi se tumačiti kao pokazatelji procesa u pulpi. Cilj ovog istraživanja bio je da se ispitaju nivoi IL9 u GCF kod pacijenata sa zubnim karijesom i da se utvrdi eventualni uticaj različitih materijala za zubne ispune na lokalnu proizvodnju IL9. Metode. U studiju je bilo uključeno 90 pacijenata, starih od 18 do 70 godina, uz primenu kriterijuma za uključivanje/isključivanje za prospektivne kliničke studije. Od ukupno šest materijala za punjenje pripremljenih kaviteta, tri je korišćeno za privremenu i tri za trajnu ispunu. Prema težini zubnog ispuna, pacijenti su bili podeljeni na tri grupe: oni sa ispunama lakšim od 0,50 g, sa ispunama od 0,50 do 1,00 g i sa ispunama težim od 1,00 g. Uzorci su uzimani iz gingivalnog sulkusa primenom tehnike filter papira. Korišćeni klinički parametri bili su indeks krvarenja, plak indeks (Silness-Lou, 0-3), gingivalni indeks (0-3) i dubina gingivalnog sulkusa. Koncentracije citokina određene su komercijalnim citomiksom. Rezultati. Težina zubne ispune ukazivala je na tendenciju opadanja vrednosti IL9, što je značilo da je veće oštećenje zuba, sa zubnom ispunom težom od 1,00 g, praćeno nižom koncentracijom IL9 u GCF. Vrednosti IL9 bile su u korelaciji sa stepenom gingivalnog indeksa i dubinom gingivalnog sulkusa, pogotovu u poodmaklom gingivitisu i izraženijim anatomskim promenama ivica zuba. Različite ispune izazivale su različite lokalne sekrecije IL9. Cink-polikarbonatni cement i amalgamska ispuna izazvale su značajan i dugotrajan pad nivoa lokalnog IL9, dok je primena tetrik-evocerama i GMA-BISK znatno povisila nivoe IL9. Zaključak. Rezultati dobijeni u ovoj studiji ukazuju da se IL9 u GCF može koristiti kao mera za reakciju odontoblasta na veličinu karijesa. Tip zubne ispune može da promeni biološku funkciju ćelija gingive i pulpe. Rezultati ove studije, takođe, ukazuju i na to da neke vrste ispuna mogu čak da ubrzaju zarastanje rane modulacijom aktivnosti makrofaga.",
publisher = "Vojnomedicinska akademija - Institut za naučne informacije, Beograd",
journal = "Vojnosanitetski pregled",
title = "Influence of dental filling material type on the concentration of interleukin 9 in the samples of gingival crevicular fluid, Uticaj tipa materijala za plombiranje zuba na koncentraciju interleukina 9 u uzorcima gingivalne sulkusne tečnosti",
volume = "73",
number = "8",
pages = "728-734",
doi = "10.2298/VSP140227054S"
}

Stefanović, V., Taso, E., Petković-Ćurčin, A., Đukić, M., Gardašević, M., Rakić, M., Xavier, S., Jović, M., Miller, K., Stanojević, I.,& Vojvodić, D.. (2016). Influence of dental filling material type on the concentration of interleukin 9 in the samples of gingival crevicular fluid. in Vojnosanitetski pregled
Vojnomedicinska akademija - Institut za naučne informacije, Beograd., 73(8), 728-734.
https://doi.org/10.2298/VSP140227054S

Stefanović V, Taso E, Petković-Ćurčin A, Đukić M, Gardašević M, Rakić M, Xavier S, Jović M, Miller K, Stanojević I, Vojvodić D. Influence of dental filling material type on the concentration of interleukin 9 in the samples of gingival crevicular fluid. in Vojnosanitetski pregled. 2016;73(8):728-734.
doi:10.2298/VSP140227054S .

Stefanović, Vladimir, Taso, Ervin, Petković-Ćurčin, Aleksandra, Đukić, Mirjana, Gardašević, Milka, Rakić, Mia, Xavier, Struillou, Jović, Milena, Miller, Karolina, Stanojević, Ivan, Vojvodić, Danilo, "Influence of dental filling material type on the concentration of interleukin 9 in the samples of gingival crevicular fluid" in Vojnosanitetski pregled, 73, no. 8 (2016):728-734,
https://doi.org/10.2298/VSP140227054S . .

TY  - JOUR
AU  - Mijušković, Mirjana
AU  - Stanojević, Ivan
AU  - Milović, Novak
AU  - Cerović, Snežana
AU  - Petrović, Dejan
AU  - Jovanović, Dragan
AU  - Aleksić, Predrag
AU  - Kovačević, Božidar
AU  - Anđelić, Tamara
AU  - Terzić, Brankica
AU  - Đukić, Mirjana
AU  - Vojvodić, Danilo
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2676
AB  - Background/Aim. Kidney injury molecule-1 (KIM-1) and aquaporin-1 (AQP-1) are potential early urinary biomarkers of clear renal cell carcinoma (cRCC). The aim of this study was to ascertain relationship between the urine concentrations KIM-1 and AQP-1 with tumor size, grade, pT stage and type of operation (radical or partial nephrectomy) in patients with cRCC. Methods. Urinary concentrations of urinary KIM-1 (uKIM-1) and urinary AQP-1 (uAQP-1) were determined by commercially available ELISA kits. The analysis included 40 patients undergoing partial or radical nephrectomy for cRCC and 40 age- and sex-matched healthy adult volunteers. Results. The median preoperative concentrations of KIM-1 in the cRCC group [0.724 ± 1.120 ng/mg urinary creatinine (Ucr)] were significantly greater compared with controls (healthy volunteers) (0.210 ± 0.082 ng/mgUcr) (p = 0.0227). Postoperatively, uKIM-1 concentration decreased significantly to control values (0.177 ± 0.099 ng/mgUcr vs 0.210 ± 0.082 ng/mgUcr, respectively). The size, grade and stage of tumor were correlated positively with preoperative uKIM-1 concentrations. Contrary to these results, concentrations of uAQP-1 in the cRCC group were significantly lower (0.111 ± 0.092 ng/mgUcr) compared with the control group (0.202 ± 0.078 ng/mgUcr) (p = 0.0014). Postoperatively, the concentrations of uAQP-1 increased progressively up to control values, approximately. We find no significant correlation between preoperative uAQP-1 concentrations and tumor size, grade and stage. Conclusion. uKIM-1 was found to be a reliable diagnostic marker of cRCC, based on its significantly increased values before and decreased values after the nephrectomy.
AB  - Uvod/Cilj. Molekul oštećenja bubrega-1 (KIM-1) i akvaporin-1 (AQP-1) su potencijalni rani biomarkeri karcinoma svetlih ćelija (cRCC). Cilj ove studije bio je da se utvrdi povezanost između koncentracija KIM-1 i AQP-1 u urinu i veličine, gradusa, stadijuma i vrste operacije (radikalna ili parcijalna nefrektomija) kod bolesnika sa cRCC. Metode. Urinarne koncentracije KIM-1 i AQP-1 određene su primenom komercijalnih ELISA kitova. Analizom je bilo obuhvaćeno 40 bolesnika koji su bili podvrgnuti parcijalnoj ili radikalnoj nefrektomiji zbog tumora bubrega i 40 zdravih odraslih ispitanika. Grupe su bile komparabilne po polu i godinama života. Rezultati. Srednja preoperativna koncentracija urinarnog KIM-1 (uKIM-1) u cRCC grupi [0,724 ± 1,120 ng/mg kreatinia u urinu (Ucr)] bila je statistički značajno viša u poređenju sa koncentracijom u kontrolnoj grupi (0,210 ± 0,082 ng/mgUcr ) (p = 0,0227). Postoperativno, koncentracija uKIM-1 značajno je padala i približavala se vrednosti u kontrolnoj grupi (0,177 ± 0,099 ng/mgUcr nasuprot 0,210 ± 0,082 ng/mgUcr). Veličina, gradus i stadijum tumora bili su u pozitivnoj korelaciji sa preoperativnim koncentracijama uKIM-1. Nasuprot ovim rezultatima, koncentracija urinarnog AQP-1 (uAQP-1) u cRCC grupi bila je značajno niža (0,111 ± 0,092 ng/mgUcr) u poređenju sa kontrolnom grupom zdravih osoba (0,202 ± 0,078 ng/mgUcr) (p = 0,0014). Postoperativno, koncentracija uAQP-1 progresivno se povećavala, približno do vrednosti u kontrolnoj grupi. Nismo našli značajnu korelaciju između preoperativnih koncentracija uAQP-1 i veličine, gradusa i stadijuma tumora. Zaključak. uKIM-1 bi mogao biti dodatni pouzdani dijagnostički marker za cRRC na osnovu njegove značajno više preoperativne koncentracije i sniženja vrednosti nakon nefrektomije.
PB  - Vojnomedicinska akademija - Institut za naučne informacije, Beograd
T2  - Vojnosanitetski pregled
T1  - Urinary KIM-1 and AQP-1 in patients with clear renal cell carcinoma: Potential noninvasive biomarkers
T1  - Molekul oštećenja bubrega-1 (KIM-1) i akvaporin-1 (AQP-1) u urinu kod bolesnika sa karcinomom svetlih ćelija bubrega - potencijalni neiznvazivni biomarkeri
VL  - 73
IS  - 3
SP  - 266
EP  - 272
DO  - 10.2298/VSP150124006M
ER  - 

@article{
author = "Mijušković, Mirjana and Stanojević, Ivan and Milović, Novak and Cerović, Snežana and Petrović, Dejan and Jovanović, Dragan and Aleksić, Predrag and Kovačević, Božidar and Anđelić, Tamara and Terzić, Brankica and Đukić, Mirjana and Vojvodić, Danilo",
year = "2016",
abstract = "Background/Aim. Kidney injury molecule-1 (KIM-1) and aquaporin-1 (AQP-1) are potential early urinary biomarkers of clear renal cell carcinoma (cRCC). The aim of this study was to ascertain relationship between the urine concentrations KIM-1 and AQP-1 with tumor size, grade, pT stage and type of operation (radical or partial nephrectomy) in patients with cRCC. Methods. Urinary concentrations of urinary KIM-1 (uKIM-1) and urinary AQP-1 (uAQP-1) were determined by commercially available ELISA kits. The analysis included 40 patients undergoing partial or radical nephrectomy for cRCC and 40 age- and sex-matched healthy adult volunteers. Results. The median preoperative concentrations of KIM-1 in the cRCC group [0.724 ± 1.120 ng/mg urinary creatinine (Ucr)] were significantly greater compared with controls (healthy volunteers) (0.210 ± 0.082 ng/mgUcr) (p = 0.0227). Postoperatively, uKIM-1 concentration decreased significantly to control values (0.177 ± 0.099 ng/mgUcr vs 0.210 ± 0.082 ng/mgUcr, respectively). The size, grade and stage of tumor were correlated positively with preoperative uKIM-1 concentrations. Contrary to these results, concentrations of uAQP-1 in the cRCC group were significantly lower (0.111 ± 0.092 ng/mgUcr) compared with the control group (0.202 ± 0.078 ng/mgUcr) (p = 0.0014). Postoperatively, the concentrations of uAQP-1 increased progressively up to control values, approximately. We find no significant correlation between preoperative uAQP-1 concentrations and tumor size, grade and stage. Conclusion. uKIM-1 was found to be a reliable diagnostic marker of cRCC, based on its significantly increased values before and decreased values after the nephrectomy., Uvod/Cilj. Molekul oštećenja bubrega-1 (KIM-1) i akvaporin-1 (AQP-1) su potencijalni rani biomarkeri karcinoma svetlih ćelija (cRCC). Cilj ove studije bio je da se utvrdi povezanost između koncentracija KIM-1 i AQP-1 u urinu i veličine, gradusa, stadijuma i vrste operacije (radikalna ili parcijalna nefrektomija) kod bolesnika sa cRCC. Metode. Urinarne koncentracije KIM-1 i AQP-1 određene su primenom komercijalnih ELISA kitova. Analizom je bilo obuhvaćeno 40 bolesnika koji su bili podvrgnuti parcijalnoj ili radikalnoj nefrektomiji zbog tumora bubrega i 40 zdravih odraslih ispitanika. Grupe su bile komparabilne po polu i godinama života. Rezultati. Srednja preoperativna koncentracija urinarnog KIM-1 (uKIM-1) u cRCC grupi [0,724 ± 1,120 ng/mg kreatinia u urinu (Ucr)] bila je statistički značajno viša u poređenju sa koncentracijom u kontrolnoj grupi (0,210 ± 0,082 ng/mgUcr ) (p = 0,0227). Postoperativno, koncentracija uKIM-1 značajno je padala i približavala se vrednosti u kontrolnoj grupi (0,177 ± 0,099 ng/mgUcr nasuprot 0,210 ± 0,082 ng/mgUcr). Veličina, gradus i stadijum tumora bili su u pozitivnoj korelaciji sa preoperativnim koncentracijama uKIM-1. Nasuprot ovim rezultatima, koncentracija urinarnog AQP-1 (uAQP-1) u cRCC grupi bila je značajno niža (0,111 ± 0,092 ng/mgUcr) u poređenju sa kontrolnom grupom zdravih osoba (0,202 ± 0,078 ng/mgUcr) (p = 0,0014). Postoperativno, koncentracija uAQP-1 progresivno se povećavala, približno do vrednosti u kontrolnoj grupi. Nismo našli značajnu korelaciju između preoperativnih koncentracija uAQP-1 i veličine, gradusa i stadijuma tumora. Zaključak. uKIM-1 bi mogao biti dodatni pouzdani dijagnostički marker za cRRC na osnovu njegove značajno više preoperativne koncentracije i sniženja vrednosti nakon nefrektomije.",
publisher = "Vojnomedicinska akademija - Institut za naučne informacije, Beograd",
journal = "Vojnosanitetski pregled",
title = "Urinary KIM-1 and AQP-1 in patients with clear renal cell carcinoma: Potential noninvasive biomarkers, Molekul oštećenja bubrega-1 (KIM-1) i akvaporin-1 (AQP-1) u urinu kod bolesnika sa karcinomom svetlih ćelija bubrega - potencijalni neiznvazivni biomarkeri",
volume = "73",
number = "3",
pages = "266-272",
doi = "10.2298/VSP150124006M"
}

Mijušković, M., Stanojević, I., Milović, N., Cerović, S., Petrović, D., Jovanović, D., Aleksić, P., Kovačević, B., Anđelić, T., Terzić, B., Đukić, M.,& Vojvodić, D.. (2016). Urinary KIM-1 and AQP-1 in patients with clear renal cell carcinoma: Potential noninvasive biomarkers. in Vojnosanitetski pregled
Vojnomedicinska akademija - Institut za naučne informacije, Beograd., 73(3), 266-272.
https://doi.org/10.2298/VSP150124006M

Mijušković M, Stanojević I, Milović N, Cerović S, Petrović D, Jovanović D, Aleksić P, Kovačević B, Anđelić T, Terzić B, Đukić M, Vojvodić D. Urinary KIM-1 and AQP-1 in patients with clear renal cell carcinoma: Potential noninvasive biomarkers. in Vojnosanitetski pregled. 2016;73(3):266-272.
doi:10.2298/VSP150124006M .

Mijušković, Mirjana, Stanojević, Ivan, Milović, Novak, Cerović, Snežana, Petrović, Dejan, Jovanović, Dragan, Aleksić, Predrag, Kovačević, Božidar, Anđelić, Tamara, Terzić, Brankica, Đukić, Mirjana, Vojvodić, Danilo, "Urinary KIM-1 and AQP-1 in patients with clear renal cell carcinoma: Potential noninvasive biomarkers" in Vojnosanitetski pregled, 73, no. 3 (2016):266-272,
https://doi.org/10.2298/VSP150124006M . .

TY  - JOUR
AU  - Karlicić, Vukoica
AU  - Vuković, Jelena
AU  - Stanojević, Ivan
AU  - Sotirović, Jelena
AU  - Perić, Alexandra
AU  - Jović, Milena
AU  - Cvijanović, Vlado
AU  - Đukić, Mirjana
AU  - Banović, Tatjana
AU  - Vojvodić, Danilo
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2641
AB  - Purpose: Advanced lung carcinoma is charasterized with fast disease progression. Interleukin (IL)10 and transforming growth factor (TGF)b1 are immunosuppressive mediators and their role in lung carcinoma pathogenesis and in the antitumor response has not yet been elucidated. The purpose of this study was to correlate IL10 and TGFb1 levels in the serum and lung tumor microcirculation with clinical stage, disease extent, histological features and TNM stage. Methods: The study included 41 lung cancer patients in clinical stage III and IV. Histological type was determined immunohistochemically, while tumor size, localization and dissemination were determined radiologically by multislice computerized tomography (MSCT). IL10 and TGFb1 levels were quantified with commercial flow cytometric test in serum and lung tumor microcirculation samples. Results: Non small cell lung cancer (NSCLC) patients had significantly elevated TGFb1 while small cell lung cancer (SCLC) patients had significantly increased IL10 in tumor microcirculation. IL10 was significantly elevated in patients with the largest tumors, as well as in patients with III clinical stage and without metastases, both in the serum and tumor microcirculation. TGFb1 was significantly increased in serum and tumor microcirculation in patients with larger tumors. We found significant correlation between these two immunosuppressive cytokines, IL10 and TGFbl, in tumor microcirculation but not in patient serum samples. Conclusion: IL10 and TGFb1 in systemic and tumor microcirculation are significantly associated with particular histological type of lung cancer, tumor size and degree of disease extent.
PB  - Balkan Union of Oncology (B.U.ON.)
T2  - Journal of BUON
T1  - Association of locally produced IL10 and TGFb1 with tumor size, histological type and presence of metastases in patients with lung carcinoma
VL  - 21
IS  - 5
SP  - 1210
EP  - 1218
UR  - https://hdl.handle.net/21.15107/rcub_farfar_2641
ER  - 

@article{
author = "Karlicić, Vukoica and Vuković, Jelena and Stanojević, Ivan and Sotirović, Jelena and Perić, Alexandra and Jović, Milena and Cvijanović, Vlado and Đukić, Mirjana and Banović, Tatjana and Vojvodić, Danilo",
year = "2016",
abstract = "Purpose: Advanced lung carcinoma is charasterized with fast disease progression. Interleukin (IL)10 and transforming growth factor (TGF)b1 are immunosuppressive mediators and their role in lung carcinoma pathogenesis and in the antitumor response has not yet been elucidated. The purpose of this study was to correlate IL10 and TGFb1 levels in the serum and lung tumor microcirculation with clinical stage, disease extent, histological features and TNM stage. Methods: The study included 41 lung cancer patients in clinical stage III and IV. Histological type was determined immunohistochemically, while tumor size, localization and dissemination were determined radiologically by multislice computerized tomography (MSCT). IL10 and TGFb1 levels were quantified with commercial flow cytometric test in serum and lung tumor microcirculation samples. Results: Non small cell lung cancer (NSCLC) patients had significantly elevated TGFb1 while small cell lung cancer (SCLC) patients had significantly increased IL10 in tumor microcirculation. IL10 was significantly elevated in patients with the largest tumors, as well as in patients with III clinical stage and without metastases, both in the serum and tumor microcirculation. TGFb1 was significantly increased in serum and tumor microcirculation in patients with larger tumors. We found significant correlation between these two immunosuppressive cytokines, IL10 and TGFbl, in tumor microcirculation but not in patient serum samples. Conclusion: IL10 and TGFb1 in systemic and tumor microcirculation are significantly associated with particular histological type of lung cancer, tumor size and degree of disease extent.",
publisher = "Balkan Union of Oncology (B.U.ON.)",
journal = "Journal of BUON",
title = "Association of locally produced IL10 and TGFb1 with tumor size, histological type and presence of metastases in patients with lung carcinoma",
volume = "21",
number = "5",
pages = "1210-1218",
url = "https://hdl.handle.net/21.15107/rcub_farfar_2641"
}

Karlicić, V., Vuković, J., Stanojević, I., Sotirović, J., Perić, A., Jović, M., Cvijanović, V., Đukić, M., Banović, T.,& Vojvodić, D.. (2016). Association of locally produced IL10 and TGFb1 with tumor size, histological type and presence of metastases in patients with lung carcinoma. in Journal of BUON
Balkan Union of Oncology (B.U.ON.)., 21(5), 1210-1218.
https://hdl.handle.net/21.15107/rcub_farfar_2641

Karlicić V, Vuković J, Stanojević I, Sotirović J, Perić A, Jović M, Cvijanović V, Đukić M, Banović T, Vojvodić D. Association of locally produced IL10 and TGFb1 with tumor size, histological type and presence of metastases in patients with lung carcinoma. in Journal of BUON. 2016;21(5):1210-1218.
https://hdl.handle.net/21.15107/rcub_farfar_2641 .

Karlicić, Vukoica, Vuković, Jelena, Stanojević, Ivan, Sotirović, Jelena, Perić, Alexandra, Jović, Milena, Cvijanović, Vlado, Đukić, Mirjana, Banović, Tatjana, Vojvodić, Danilo, "Association of locally produced IL10 and TGFb1 with tumor size, histological type and presence of metastases in patients with lung carcinoma" in Journal of BUON, 21, no. 5 (2016):1210-1218,
https://hdl.handle.net/21.15107/rcub_farfar_2641 .

TY  - JOUR
AU  - Đurić, Ana
AU  - Begić, Aida
AU  - Gobeljić, Borko
AU  - Stanojević, Ivan
AU  - Ninković, Milica
AU  - Vojvodić, Danilo
AU  - Pantelić, Ana
AU  - Zebić, Goran
AU  - Prokić, Vera
AU  - Dejanović, Bratislav
AU  - Stojanović, Ivana
AU  - Pavlica, Marina
AU  - Đukić, Dušan
AU  - Saso, Luciano
AU  - Đurđević, Dragan
AU  - Pavlović, Miloš
AU  - Topić, Aleksandra
AU  - Vujanović, Dragana
AU  - Stevnović, Ivana
AU  - Đukić, Mirjana
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2364
AB  - The objective of our study was to examine testicular toxicity of cadmium (Cd), focusing on oxidative stress (OS), essential metals and androgenic status and morphological changes. Male Wistar rats [controls and four Cd-subgroups (n = 6) organized according to the exposure (1, 3, 10 and 21 days)] were intraperitoneally (i.p.) treated with 1 mg CdCl2/kg/day. Testicular Cd deposition was noticed from the 1st day. After 10 and 21 days, copper (Cu) and iron (Fe) increased by 60-109% and 43-67%, respectively, while zinc (Zn) decreased by 24-33%. During 121 days of the exposure, decrease in testicular total superoxide dismutase (SOD) and total glutathione-s-transferase (GST) activities occurred gradually by 30-78% and 15-84%, respectively, while superoxide anion radical (O-2(center dot-)) increased gradually by 114-271%. After 10-21 days, decrease in testicular catalase (CAT) activity appeared by 13-31%. After 21 days, malondialdehyde (MDA) decreased by 44% and the ratio of oxidized glutathione/reduced glutathione (GSSG/GSH) increased by 130% in testes of the rats exposed to Cd. Additionally, decreased testicular testosterone level and the relative testes mass, along with induced microscopic and macroscopic changes were occured, what can be explained as the consequence of instantly developed OS, impaired essential metals status and Cd testicular deposition.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Food and Chemical Toxicology
T1  - Oxidative stress, bioelements and androgen status in testes of rats subacutely exposed to cadmium
VL  - 86
SP  - 25
EP  - 33
DO  - 10.1016/j.fct.2015.09.004
ER  - 

@article{
author = "Đurić, Ana and Begić, Aida and Gobeljić, Borko and Stanojević, Ivan and Ninković, Milica and Vojvodić, Danilo and Pantelić, Ana and Zebić, Goran and Prokić, Vera and Dejanović, Bratislav and Stojanović, Ivana and Pavlica, Marina and Đukić, Dušan and Saso, Luciano and Đurđević, Dragan and Pavlović, Miloš and Topić, Aleksandra and Vujanović, Dragana and Stevnović, Ivana and Đukić, Mirjana",
year = "2015",
abstract = "The objective of our study was to examine testicular toxicity of cadmium (Cd), focusing on oxidative stress (OS), essential metals and androgenic status and morphological changes. Male Wistar rats [controls and four Cd-subgroups (n = 6) organized according to the exposure (1, 3, 10 and 21 days)] were intraperitoneally (i.p.) treated with 1 mg CdCl2/kg/day. Testicular Cd deposition was noticed from the 1st day. After 10 and 21 days, copper (Cu) and iron (Fe) increased by 60-109% and 43-67%, respectively, while zinc (Zn) decreased by 24-33%. During 121 days of the exposure, decrease in testicular total superoxide dismutase (SOD) and total glutathione-s-transferase (GST) activities occurred gradually by 30-78% and 15-84%, respectively, while superoxide anion radical (O-2(center dot-)) increased gradually by 114-271%. After 10-21 days, decrease in testicular catalase (CAT) activity appeared by 13-31%. After 21 days, malondialdehyde (MDA) decreased by 44% and the ratio of oxidized glutathione/reduced glutathione (GSSG/GSH) increased by 130% in testes of the rats exposed to Cd. Additionally, decreased testicular testosterone level and the relative testes mass, along with induced microscopic and macroscopic changes were occured, what can be explained as the consequence of instantly developed OS, impaired essential metals status and Cd testicular deposition.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Food and Chemical Toxicology",
title = "Oxidative stress, bioelements and androgen status in testes of rats subacutely exposed to cadmium",
volume = "86",
pages = "25-33",
doi = "10.1016/j.fct.2015.09.004"
}

Đurić, A., Begić, A., Gobeljić, B., Stanojević, I., Ninković, M., Vojvodić, D., Pantelić, A., Zebić, G., Prokić, V., Dejanović, B., Stojanović, I., Pavlica, M., Đukić, D., Saso, L., Đurđević, D., Pavlović, M., Topić, A., Vujanović, D., Stevnović, I.,& Đukić, M.. (2015). Oxidative stress, bioelements and androgen status in testes of rats subacutely exposed to cadmium. in Food and Chemical Toxicology
Pergamon-Elsevier Science Ltd, Oxford., 86, 25-33.
https://doi.org/10.1016/j.fct.2015.09.004

Đurić A, Begić A, Gobeljić B, Stanojević I, Ninković M, Vojvodić D, Pantelić A, Zebić G, Prokić V, Dejanović B, Stojanović I, Pavlica M, Đukić D, Saso L, Đurđević D, Pavlović M, Topić A, Vujanović D, Stevnović I, Đukić M. Oxidative stress, bioelements and androgen status in testes of rats subacutely exposed to cadmium. in Food and Chemical Toxicology. 2015;86:25-33.
doi:10.1016/j.fct.2015.09.004 .

Đurić, Ana, Begić, Aida, Gobeljić, Borko, Stanojević, Ivan, Ninković, Milica, Vojvodić, Danilo, Pantelić, Ana, Zebić, Goran, Prokić, Vera, Dejanović, Bratislav, Stojanović, Ivana, Pavlica, Marina, Đukić, Dušan, Saso, Luciano, Đurđević, Dragan, Pavlović, Miloš, Topić, Aleksandra, Vujanović, Dragana, Stevnović, Ivana, Đukić, Mirjana, "Oxidative stress, bioelements and androgen status in testes of rats subacutely exposed to cadmium" in Food and Chemical Toxicology, 86 (2015):25-33,
https://doi.org/10.1016/j.fct.2015.09.004 . .

TY  - JOUR
AU  - Pavlović, Dragana R.
AU  - Lakušić, Branislava
AU  - Kitić, Dušanka
AU  - Milutinović, Milica
AU  - Kostić, Milica
AU  - Miladinović, Bojana
AU  - Kovačević, Nada
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2239
AB  - Uvae ursi folium (Arctostaphylos uva ursi, Ericaceae) is the best known and most widely used herbal urinary antiseptic. In traditional medicine, other Ericaceae species are also used for the treatment of urinary tract infections. The present study investigates antimicrobial activity of five species of Ericaceae family native to the Balkan Peninsula: Arbutus unedo, Bruckenthalia spiculifolia, Calluna vulgaris, Erica arborea and Erica carnea. Ethanolic extracts were tested against 10 different gram-positive and gram-negative bacteria by the disc diffusion technique, where standard tetracycline, streptomycin and penicillin discs and discs containing crystal violet (1 mg/ml) and solvent (70.0% v/v ethanol) were used as controls. The most prominent antibacterial effect was achieved on Staphylococcus aureus with extracts of Calluna vulgaris and Erica carnea. Tested samples showed no activity against the gram-negative strains Pseudomonas aeruginosa, Escherichia coli and Klebsiella pneumoniae. Inhibitory effects on the growth of gram-positive bacteria were more potent. The exception is Arbutus unedo ethanol extract which exhibited certain activity against a laboratory strain of wild Escherichia coli. Antimicrobial activity of the ethanolic extracts against 10 tested strains of bacteria in disc diffusion assay was generally weak, even for sample in which HPLC determination confirmed the presence of arbutin (secondary metabolite responsible for most of the antibacterial activity of Uvaeursi folium).
AB  - Najpoznatiji i najviše korišćeni biljni uroantiseptik je list uve, Uvaeursi folium (Arctostaphylos uva ursi, Ericaceae). U tradicionalnoj medicini često se i druge vrste familije Ericaceae spominju u lečenju urinarnih infekcija. Provera antimikrobne aktivnosti izvršena je za sledeće biljne vrste ove familije koje samostalno rastu u flori Balkanskog poluostrva: Arbutus unedo, Bruckentalia spiculifolia, Calluna vulgaris, Erica arborea i Erica carnea. Antimikrobna aktivnost etanolnih ekstrakata listova ispitivana je diskdifuzionom metodom, korišćenjem 10 sojeva gram pozitivnih i gram negativnih bakterija, pri čemu su kao kontrole korišćeni standardni diskovi tetraciklina, streptomicina i penicilina, kao i diskovi sa rastvorom kristalvioleta (1 mg/ml) i rastvarača (70.0% v/v etanol). Pseudomonas aeruginosa, Escherichia coli i Klebsiella pneumoniae nisu osetljive ni na jedan od ispitivanih ekstrakata. Kao najbolji rezultati mogu se izdvojiti aktivnosti ekstrakta Calluna vulgaris i Erica carnea na bakterijski soj Staphylococcus aureus. Testirani uzorci uglavnom ispoljavaju antimikrobnu aktivnost prema gram pozitivnim sojevima. Izuzetak je etanolni ekstrakt vrste Arbutus unedo, koji ispoljava određenu antimikrobnu aktivnost prema laboratorijskom soju Escherichia coli. Antimikrobna aktivnost etanolnih ekstrakata prema 10 testiranih bakterijskih sojeva u diskdifuzionoj metodi je veoma slaba, čak i za uzorak za koji je HPLC analizom utvrđeno prisustvo arbutina (sekundarni metabolit odgovoran za uroantiseptično dejstvo lista uve).
PB  - Univerzitet u Nišu - Medicinski fakultet, Niš
T2  - Acta Facultatis Medicae Naissensis
T1  - Antimicrobial activity of selected plant species of genera Arbutus L., Bruckenthalia Rchb., Calluna Salisb. and Erica L. (Ericaceae)
T1  - Antimikrobna aktivnost odabranih biljnih vrsta rodova Arbutus L., Bruckenthalia rchb., Calluna salisb. i Erica L. (Ericaceae)
VL  - 31
IS  - 1
SP  - 81
EP  - 85
DO  - 10.2478/afmnai-2014-0009
ER  - 

@article{
author = "Pavlović, Dragana R. and Lakušić, Branislava and Kitić, Dušanka and Milutinović, Milica and Kostić, Milica and Miladinović, Bojana and Kovačević, Nada",
year = "2014",
abstract = "Uvae ursi folium (Arctostaphylos uva ursi, Ericaceae) is the best known and most widely used herbal urinary antiseptic. In traditional medicine, other Ericaceae species are also used for the treatment of urinary tract infections. The present study investigates antimicrobial activity of five species of Ericaceae family native to the Balkan Peninsula: Arbutus unedo, Bruckenthalia spiculifolia, Calluna vulgaris, Erica arborea and Erica carnea. Ethanolic extracts were tested against 10 different gram-positive and gram-negative bacteria by the disc diffusion technique, where standard tetracycline, streptomycin and penicillin discs and discs containing crystal violet (1 mg/ml) and solvent (70.0% v/v ethanol) were used as controls. The most prominent antibacterial effect was achieved on Staphylococcus aureus with extracts of Calluna vulgaris and Erica carnea. Tested samples showed no activity against the gram-negative strains Pseudomonas aeruginosa, Escherichia coli and Klebsiella pneumoniae. Inhibitory effects on the growth of gram-positive bacteria were more potent. The exception is Arbutus unedo ethanol extract which exhibited certain activity against a laboratory strain of wild Escherichia coli. Antimicrobial activity of the ethanolic extracts against 10 tested strains of bacteria in disc diffusion assay was generally weak, even for sample in which HPLC determination confirmed the presence of arbutin (secondary metabolite responsible for most of the antibacterial activity of Uvaeursi folium)., Najpoznatiji i najviše korišćeni biljni uroantiseptik je list uve, Uvaeursi folium (Arctostaphylos uva ursi, Ericaceae). U tradicionalnoj medicini često se i druge vrste familije Ericaceae spominju u lečenju urinarnih infekcija. Provera antimikrobne aktivnosti izvršena je za sledeće biljne vrste ove familije koje samostalno rastu u flori Balkanskog poluostrva: Arbutus unedo, Bruckentalia spiculifolia, Calluna vulgaris, Erica arborea i Erica carnea. Antimikrobna aktivnost etanolnih ekstrakata listova ispitivana je diskdifuzionom metodom, korišćenjem 10 sojeva gram pozitivnih i gram negativnih bakterija, pri čemu su kao kontrole korišćeni standardni diskovi tetraciklina, streptomicina i penicilina, kao i diskovi sa rastvorom kristalvioleta (1 mg/ml) i rastvarača (70.0% v/v etanol). Pseudomonas aeruginosa, Escherichia coli i Klebsiella pneumoniae nisu osetljive ni na jedan od ispitivanih ekstrakata. Kao najbolji rezultati mogu se izdvojiti aktivnosti ekstrakta Calluna vulgaris i Erica carnea na bakterijski soj Staphylococcus aureus. Testirani uzorci uglavnom ispoljavaju antimikrobnu aktivnost prema gram pozitivnim sojevima. Izuzetak je etanolni ekstrakt vrste Arbutus unedo, koji ispoljava određenu antimikrobnu aktivnost prema laboratorijskom soju Escherichia coli. Antimikrobna aktivnost etanolnih ekstrakata prema 10 testiranih bakterijskih sojeva u diskdifuzionoj metodi je veoma slaba, čak i za uzorak za koji je HPLC analizom utvrđeno prisustvo arbutina (sekundarni metabolit odgovoran za uroantiseptično dejstvo lista uve).",
publisher = "Univerzitet u Nišu - Medicinski fakultet, Niš",
journal = "Acta Facultatis Medicae Naissensis",
title = "Antimicrobial activity of selected plant species of genera Arbutus L., Bruckenthalia Rchb., Calluna Salisb. and Erica L. (Ericaceae), Antimikrobna aktivnost odabranih biljnih vrsta rodova Arbutus L., Bruckenthalia rchb., Calluna salisb. i Erica L. (Ericaceae)",
volume = "31",
number = "1",
pages = "81-85",
doi = "10.2478/afmnai-2014-0009"
}

Pavlović, D. R., Lakušić, B., Kitić, D., Milutinović, M., Kostić, M., Miladinović, B.,& Kovačević, N.. (2014). Antimicrobial activity of selected plant species of genera Arbutus L., Bruckenthalia Rchb., Calluna Salisb. and Erica L. (Ericaceae). in Acta Facultatis Medicae Naissensis
Univerzitet u Nišu - Medicinski fakultet, Niš., 31(1), 81-85.
https://doi.org/10.2478/afmnai-2014-0009

Pavlović DR, Lakušić B, Kitić D, Milutinović M, Kostić M, Miladinović B, Kovačević N. Antimicrobial activity of selected plant species of genera Arbutus L., Bruckenthalia Rchb., Calluna Salisb. and Erica L. (Ericaceae). in Acta Facultatis Medicae Naissensis. 2014;31(1):81-85.
doi:10.2478/afmnai-2014-0009 .

Pavlović, Dragana R., Lakušić, Branislava, Kitić, Dušanka, Milutinović, Milica, Kostić, Milica, Miladinović, Bojana, Kovačević, Nada, "Antimicrobial activity of selected plant species of genera Arbutus L., Bruckenthalia Rchb., Calluna Salisb. and Erica L. (Ericaceae)" in Acta Facultatis Medicae Naissensis, 31, no. 1 (2014):81-85,
https://doi.org/10.2478/afmnai-2014-0009 . .

TY  - JOUR
AU  - Pavlović, Dragana R.
AU  - Tasić-Kostov, Marija
AU  - Marčetić, Mirjana
AU  - Lakušić, Branislava
AU  - Kitić, Dušanka
AU  - Savić, Snežana
AU  - Kovačević, Nada
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1885
AB  - Arbutus unedo, Bruckentalia spiculifolia, Calluna vulgaris, Erica arborea and Erica carnea possess strong antioxidant activity and they are traditionally used for the treatment of various skin diseases. The present study investigated the total phenylpropanoid content, antioxidant properties and phenolic composition of dry leaf ethanol extracts of these species. Furthermore, the in vivo effects of gels, each containing 2% of a single extract were tested on the artificially irritated human skin using the objective methods of skin biophysical measurements (erythema index (EI), pH of the skin and electrical capacitance (EC) as a measure of skin hydration level were assessed). In total, 13 components were identified by RP-HPLC coupled with DAD detection; quercitrin, quercetin 3-O-glucoside and gallic acid were detected in all investigated samples while chlorogenic acid and quercetin were present in 4 samples. Regarding the in vivo study, all investigated gels significantly decreased the skin irritation level and reversed the pH of the skin disturbed by preirritation, while results were contradictory regarding skin hydration measurements. In conclusion, the assessed in vivo topical effects of investigated extracts matched well with their phenylpropanoid content as well as with assessed antioxidant activities.
PB  - Innovhub Ssi-Area Ssog, Milano
T2  - RSC Advances
T1  - Evaluation of in vivo effects on surfactant-irritated human skin, antioxidant properties and phenolic composition of five Ericaceae species extracts
VL  - 90
IS  - 4
SP  - 255
EP  - 264
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1885
ER  - 

@article{
author = "Pavlović, Dragana R. and Tasić-Kostov, Marija and Marčetić, Mirjana and Lakušić, Branislava and Kitić, Dušanka and Savić, Snežana and Kovačević, Nada",
year = "2013",
abstract = "Arbutus unedo, Bruckentalia spiculifolia, Calluna vulgaris, Erica arborea and Erica carnea possess strong antioxidant activity and they are traditionally used for the treatment of various skin diseases. The present study investigated the total phenylpropanoid content, antioxidant properties and phenolic composition of dry leaf ethanol extracts of these species. Furthermore, the in vivo effects of gels, each containing 2% of a single extract were tested on the artificially irritated human skin using the objective methods of skin biophysical measurements (erythema index (EI), pH of the skin and electrical capacitance (EC) as a measure of skin hydration level were assessed). In total, 13 components were identified by RP-HPLC coupled with DAD detection; quercitrin, quercetin 3-O-glucoside and gallic acid were detected in all investigated samples while chlorogenic acid and quercetin were present in 4 samples. Regarding the in vivo study, all investigated gels significantly decreased the skin irritation level and reversed the pH of the skin disturbed by preirritation, while results were contradictory regarding skin hydration measurements. In conclusion, the assessed in vivo topical effects of investigated extracts matched well with their phenylpropanoid content as well as with assessed antioxidant activities.",
publisher = "Innovhub Ssi-Area Ssog, Milano",
journal = "RSC Advances",
title = "Evaluation of in vivo effects on surfactant-irritated human skin, antioxidant properties and phenolic composition of five Ericaceae species extracts",
volume = "90",
number = "4",
pages = "255-264",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1885"
}

Pavlović, D. R., Tasić-Kostov, M., Marčetić, M., Lakušić, B., Kitić, D., Savić, S.,& Kovačević, N.. (2013). Evaluation of in vivo effects on surfactant-irritated human skin, antioxidant properties and phenolic composition of five Ericaceae species extracts. in RSC Advances
Innovhub Ssi-Area Ssog, Milano., 90(4), 255-264.
https://hdl.handle.net/21.15107/rcub_farfar_1885

Pavlović DR, Tasić-Kostov M, Marčetić M, Lakušić B, Kitić D, Savić S, Kovačević N. Evaluation of in vivo effects on surfactant-irritated human skin, antioxidant properties and phenolic composition of five Ericaceae species extracts. in RSC Advances. 2013;90(4):255-264.
https://hdl.handle.net/21.15107/rcub_farfar_1885 .

Pavlović, Dragana R., Tasić-Kostov, Marija, Marčetić, Mirjana, Lakušić, Branislava, Kitić, Dušanka, Savić, Snežana, Kovačević, Nada, "Evaluation of in vivo effects on surfactant-irritated human skin, antioxidant properties and phenolic composition of five Ericaceae species extracts" in RSC Advances, 90, no. 4 (2013):255-264,
https://hdl.handle.net/21.15107/rcub_farfar_1885 .

TY  - JOUR
AU  - Đurđević, Dragan
AU  - Đukić, Mirjana
AU  - Ninković, Milica
AU  - Stevanović, Ivana
AU  - Jovanović, Marina
AU  - Vasić, Una
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2062
AB  - Diquat (DQ) neurotoxicity mechanisms are unknown, although, it's systemic toxicity is mediated by free radical reactions. The role of glutathione cycle was assessed by glutathione reductase (GR) applied in the pre-treatment of DQ poisoning. Wistar rats were used and tested compounds were administered intrastriatally (i.s.) in one single dose. Total glutathione (tGSH), glutathione disulfide (GSSG) and glutathione peroxidase (GPx) were measured in the vulnerable brain regions (VBRs) (striatum, hippocampus and cortex), at 30 minutes, 24 hours and 7 days post treatment. Results from the intact and the sham operated groups were not statistically different. Rapid spatial spreading of oxidative stress was confirmed in the examined VBRs.. Mortality (30-40%, within 24hrs) and signs of lethargy were observed in the DQ group. Activity of GPx activity was elevated and GSSG/GSH was higher in the examined VBRs during the experiment, compared to the controls. The i.s. pre-treatment with GR achieved neuroprotective role against DQ induced neurotoxicity, based on animal survival, absence of lethargy and decreased GPx activity and GSSG/GSH in the examined VBRs during the experiment, compared to the DQ group. Our results confirmed that oxidation of GSH was the reason for the reduced antioxidative defense against DQ neurotoxicity.
AB  - Mehanizmi neurotoksičnosti dikvata (DK) su nepoznati, mada se zna da je sistemska toksičnost posredovana reakcijama slobodnih radikala. Uloga glutationskog ciklusa je isptivana primenom glutation reduktaze (GR) u predtretmanu trovanja DK. Wistar pacovi su korišćeni i testirana jedinjenja intrastrjiatalno (i.s.) primenjena u jednokratnoj dozi. Ukupni glutation (tGSH), glutation-disulfid (GSSG) i aktivnost glutation peroksidaze (GPx) su mereni u selektivno osetljivim regionima mozga (strijatum, hipokampus i korteks), 30. minuta, 24. sata i 7. dana posle tretmana. Rezultati netretiranih (intaktna grupa) i lažno operisanih pacova se ne razlikuju statistički. Vremensko i prostorno širenje oksidativnog stresa je potvrđeno kod ispitivanih moždanih struktura. Mortalitet (30-40%, u roku od 24 casa) i znaci letargije su uočeni u samo u DK grupi. Statistički povećana aktivnost GPx, kao i odnosa GSSG/GSH u ispitivanim moždanim strukturama tokom eksperimenta, potvrđuje oksidativno narušenu ravnotežu i oštećenja moždanog tkiva. Predtretman i.s. sa GR je ispoljio neurozaštitni efekat od neurotoksičnosti DK, bazirano na preživljavanju životinja, odsustvu letargije i smanjenoj aktivnost GPx i odnosa GSSG / GSH ispitivanih moždanih struktura tokom eksperimenta, u odnosu na DK grupu. Naši rezultati ukazuju da je oksidacija GSH kljucna za smanjenje antioksidativne odbrane od DK neurotoksicnosti.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta veterinaria
T1  - Glutathione cycle in diquat neurotoxicity: Assessed by intrastriatal pre-treatment with glutathione reductase
T1  - Uloga glutationskog ciklusa u neurotoksičnosti dikvata - ispitivano primenom intrastrijatalnog predtretmana sa glutation reduktazom
VL  - 63
IS  - 2-3
SP  - 159
EP  - 175
DO  - 10.2298/AVB1303159D
ER  - 

@article{
author = "Đurđević, Dragan and Đukić, Mirjana and Ninković, Milica and Stevanović, Ivana and Jovanović, Marina and Vasić, Una",
year = "2013",
abstract = "Diquat (DQ) neurotoxicity mechanisms are unknown, although, it's systemic toxicity is mediated by free radical reactions. The role of glutathione cycle was assessed by glutathione reductase (GR) applied in the pre-treatment of DQ poisoning. Wistar rats were used and tested compounds were administered intrastriatally (i.s.) in one single dose. Total glutathione (tGSH), glutathione disulfide (GSSG) and glutathione peroxidase (GPx) were measured in the vulnerable brain regions (VBRs) (striatum, hippocampus and cortex), at 30 minutes, 24 hours and 7 days post treatment. Results from the intact and the sham operated groups were not statistically different. Rapid spatial spreading of oxidative stress was confirmed in the examined VBRs.. Mortality (30-40%, within 24hrs) and signs of lethargy were observed in the DQ group. Activity of GPx activity was elevated and GSSG/GSH was higher in the examined VBRs during the experiment, compared to the controls. The i.s. pre-treatment with GR achieved neuroprotective role against DQ induced neurotoxicity, based on animal survival, absence of lethargy and decreased GPx activity and GSSG/GSH in the examined VBRs during the experiment, compared to the DQ group. Our results confirmed that oxidation of GSH was the reason for the reduced antioxidative defense against DQ neurotoxicity., Mehanizmi neurotoksičnosti dikvata (DK) su nepoznati, mada se zna da je sistemska toksičnost posredovana reakcijama slobodnih radikala. Uloga glutationskog ciklusa je isptivana primenom glutation reduktaze (GR) u predtretmanu trovanja DK. Wistar pacovi su korišćeni i testirana jedinjenja intrastrjiatalno (i.s.) primenjena u jednokratnoj dozi. Ukupni glutation (tGSH), glutation-disulfid (GSSG) i aktivnost glutation peroksidaze (GPx) su mereni u selektivno osetljivim regionima mozga (strijatum, hipokampus i korteks), 30. minuta, 24. sata i 7. dana posle tretmana. Rezultati netretiranih (intaktna grupa) i lažno operisanih pacova se ne razlikuju statistički. Vremensko i prostorno širenje oksidativnog stresa je potvrđeno kod ispitivanih moždanih struktura. Mortalitet (30-40%, u roku od 24 casa) i znaci letargije su uočeni u samo u DK grupi. Statistički povećana aktivnost GPx, kao i odnosa GSSG/GSH u ispitivanim moždanim strukturama tokom eksperimenta, potvrđuje oksidativno narušenu ravnotežu i oštećenja moždanog tkiva. Predtretman i.s. sa GR je ispoljio neurozaštitni efekat od neurotoksičnosti DK, bazirano na preživljavanju životinja, odsustvu letargije i smanjenoj aktivnost GPx i odnosa GSSG / GSH ispitivanih moždanih struktura tokom eksperimenta, u odnosu na DK grupu. Naši rezultati ukazuju da je oksidacija GSH kljucna za smanjenje antioksidativne odbrane od DK neurotoksicnosti.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta veterinaria",
title = "Glutathione cycle in diquat neurotoxicity: Assessed by intrastriatal pre-treatment with glutathione reductase, Uloga glutationskog ciklusa u neurotoksičnosti dikvata - ispitivano primenom intrastrijatalnog predtretmana sa glutation reduktazom",
volume = "63",
number = "2-3",
pages = "159-175",
doi = "10.2298/AVB1303159D"
}

Đurđević, D., Đukić, M., Ninković, M., Stevanović, I., Jovanović, M.,& Vasić, U.. (2013). Glutathione cycle in diquat neurotoxicity: Assessed by intrastriatal pre-treatment with glutathione reductase. in Acta veterinaria
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 63(2-3), 159-175.
https://doi.org/10.2298/AVB1303159D

Đurđević D, Đukić M, Ninković M, Stevanović I, Jovanović M, Vasić U. Glutathione cycle in diquat neurotoxicity: Assessed by intrastriatal pre-treatment with glutathione reductase. in Acta veterinaria. 2013;63(2-3):159-175.
doi:10.2298/AVB1303159D .

Đurđević, Dragan, Đukić, Mirjana, Ninković, Milica, Stevanović, Ivana, Jovanović, Marina, Vasić, Una, "Glutathione cycle in diquat neurotoxicity: Assessed by intrastriatal pre-treatment with glutathione reductase" in Acta veterinaria, 63, no. 2-3 (2013):159-175,
https://doi.org/10.2298/AVB1303159D . .

TY  - JOUR
AU  - Mirković, Dušica
AU  - Ibrić, Svetlana
AU  - Antunović, Mirjana
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2052
AB  - Background/Aim. Parenteral nutrition as a specific aspect of providing nutritients still remains a permanent topic of both theoretical and experimental research. Total parenteral nutrition (TPN) admixtures have complex contents making difficult to maintain their stability. The most critical parameter is the diameter of a lipid droplet, i.e. droplet size distribution. It is recommended that droplet size should not be more than 5 􀆬m and that the presence of greater droplets should not exceed the value of 0.05%. Lipid droplets size is affected particularly by electrolyte addition, especially polyvalent cations. There is a danger of the added electrolytes interaction with lipid droplets which leads to their aggregation and negative effects upon the admixtures stability. The aim of this study was to assess the effect of added electrolyte and lipid phase quantity on the admixture stability. Methods. Electrolytes were added to the studied admixture of a defined basic formulation contents in accordance with recommendations from the literature. Droplets size measurements were performed using the method of laser diffraction with a laser particles analyzer. Effects of independent variables were calculated and evaluated using commercial software. Na+, K+, Ca2+ and Mg2+ concentrations, as well as the quantity of fat phase were chosen as studied factors, i.e. independent variables. The system response, or dependent variable was the median of droplets size. Each of the factors was varied at two levels, higher (+1) and lower (-1), according to the 25-2 fractional factorial design. Results. The study suggested the presence of relative uniformity of the results of all the measurements regardless of the quantity of added electrolytes and lipid phase. It was shown that undoubtedly there is the influence of 2-valent cations (calcium and magnesium) upon lipid droplets size, which is in a direct correlation with theoretical assumption. Conclusion. Within a 72-hour testing period there was no significant increase in droplet size, i.e. the studied admixtures remained stable considering droplet size median as the criterion of stability.
AB  - Uvod/Cilj. Parenteralna ishrana, kao specifičan vid nadoknade hranljivih materija, i dalje predstavlja stalnu temu teorijskog i eksperimentalnog izučavanja. Složeni sastav smeše za totalnu parenteralnu ishranu (TPI) otežava održanje njihove stabilnosti. Najkritičniji parameter je dijametar lipidnih kapi, odnosno raspodela veličina kapi. Postoji preporuka da veličina kapi ne bi trebalo da prelazi 5 μm i da zastupljenost većih kapi ne prelazi vrednost od 0,05%. Na veličinu lipidnih kapi poseban uticaj ima dodavanje elektrolita, naročito viševalentnih katjona. Postoji opasnost da dodati elektroliti interreaguju sa lipidnim kapima, što dovodi do njihovog spajanja i ima negativan uticaj na stabilnost smeša. Cilj ovog rada bio je da se istraži kako dodati elektroliti i količina lipidne faze utiču na stabilnost ovih smeša. Metode. Ispitivanoj smeši sa definisanim sastavom osnovne formulacije izrađenoj u bolničkoj apoteci, dodavani su elektroliti na osnovu preporuka iz literature. Merenje veličine kapi vršeno je metodom laserske difrakcije pomoću laserskog analizatora čestica. Uticaj nezavisno promenljivih je procenjen i izračunat primenom komercijalnog softvera. Kao nezavisno promenljive, u svojstvu ispitivanih faktora izabrani su koncentracije Na+, K+, Ca2+ i Mg2+, kao i količina masne faze. Odgovor sistema, ili zavisno promenljiva veličina, bila je medijana veličine kapi. Svaki od faktora variran je na dva nivoa, gornji (+1) i donji (-1), odnosno primenjen je 25-2 frakcioni faktorijalni dizajn. Rezultati. Istraživanje je pokazalo da nezavisno od količine dodatih elektrolita i količine lipidne faze postoji relativna ujednačenost rezultata za sva merenja. Analiza pojedinačnih faktora ukazuje na nesumnjiv uticaj dvovalentnih katjona (kalcijuma i magnezijuma) na veličinu lipidnih kapi, što je u direktnoj korelaciji sa teoretskim postavkama. Zaključak: Tokom ispitivanog 72-časovnog perioda nije bilo značajnog povećanja veličine kapi, odnosno ispitivana smeša ostala je stabilna sa stanovišta medijane veličine kapi kao kriterijuma stabilnosti.
PB  - Vojnomedicinska akademija - Institut za naučne informacije, Beograd
T2  - Vojnosanitetski pregled
T1  - Quality assessment of total parenteral nutrition admixtures by the use of fractional factorial design
T1  - Analiza kvaliteta smeša za totalnu parenteralnu ishranu primenom delimičnog faktorijalnog dizajna
VL  - 70
IS  - 4
SP  - 374
EP  - 379
DO  - 10.2298/VSP1304374M
ER  - 

@article{
author = "Mirković, Dušica and Ibrić, Svetlana and Antunović, Mirjana",
year = "2013",
abstract = "Background/Aim. Parenteral nutrition as a specific aspect of providing nutritients still remains a permanent topic of both theoretical and experimental research. Total parenteral nutrition (TPN) admixtures have complex contents making difficult to maintain their stability. The most critical parameter is the diameter of a lipid droplet, i.e. droplet size distribution. It is recommended that droplet size should not be more than 5 􀆬m and that the presence of greater droplets should not exceed the value of 0.05%. Lipid droplets size is affected particularly by electrolyte addition, especially polyvalent cations. There is a danger of the added electrolytes interaction with lipid droplets which leads to their aggregation and negative effects upon the admixtures stability. The aim of this study was to assess the effect of added electrolyte and lipid phase quantity on the admixture stability. Methods. Electrolytes were added to the studied admixture of a defined basic formulation contents in accordance with recommendations from the literature. Droplets size measurements were performed using the method of laser diffraction with a laser particles analyzer. Effects of independent variables were calculated and evaluated using commercial software. Na+, K+, Ca2+ and Mg2+ concentrations, as well as the quantity of fat phase were chosen as studied factors, i.e. independent variables. The system response, or dependent variable was the median of droplets size. Each of the factors was varied at two levels, higher (+1) and lower (-1), according to the 25-2 fractional factorial design. Results. The study suggested the presence of relative uniformity of the results of all the measurements regardless of the quantity of added electrolytes and lipid phase. It was shown that undoubtedly there is the influence of 2-valent cations (calcium and magnesium) upon lipid droplets size, which is in a direct correlation with theoretical assumption. Conclusion. Within a 72-hour testing period there was no significant increase in droplet size, i.e. the studied admixtures remained stable considering droplet size median as the criterion of stability., Uvod/Cilj. Parenteralna ishrana, kao specifičan vid nadoknade hranljivih materija, i dalje predstavlja stalnu temu teorijskog i eksperimentalnog izučavanja. Složeni sastav smeše za totalnu parenteralnu ishranu (TPI) otežava održanje njihove stabilnosti. Najkritičniji parameter je dijametar lipidnih kapi, odnosno raspodela veličina kapi. Postoji preporuka da veličina kapi ne bi trebalo da prelazi 5 μm i da zastupljenost većih kapi ne prelazi vrednost od 0,05%. Na veličinu lipidnih kapi poseban uticaj ima dodavanje elektrolita, naročito viševalentnih katjona. Postoji opasnost da dodati elektroliti interreaguju sa lipidnim kapima, što dovodi do njihovog spajanja i ima negativan uticaj na stabilnost smeša. Cilj ovog rada bio je da se istraži kako dodati elektroliti i količina lipidne faze utiču na stabilnost ovih smeša. Metode. Ispitivanoj smeši sa definisanim sastavom osnovne formulacije izrađenoj u bolničkoj apoteci, dodavani su elektroliti na osnovu preporuka iz literature. Merenje veličine kapi vršeno je metodom laserske difrakcije pomoću laserskog analizatora čestica. Uticaj nezavisno promenljivih je procenjen i izračunat primenom komercijalnog softvera. Kao nezavisno promenljive, u svojstvu ispitivanih faktora izabrani su koncentracije Na+, K+, Ca2+ i Mg2+, kao i količina masne faze. Odgovor sistema, ili zavisno promenljiva veličina, bila je medijana veličine kapi. Svaki od faktora variran je na dva nivoa, gornji (+1) i donji (-1), odnosno primenjen je 25-2 frakcioni faktorijalni dizajn. Rezultati. Istraživanje je pokazalo da nezavisno od količine dodatih elektrolita i količine lipidne faze postoji relativna ujednačenost rezultata za sva merenja. Analiza pojedinačnih faktora ukazuje na nesumnjiv uticaj dvovalentnih katjona (kalcijuma i magnezijuma) na veličinu lipidnih kapi, što je u direktnoj korelaciji sa teoretskim postavkama. Zaključak: Tokom ispitivanog 72-časovnog perioda nije bilo značajnog povećanja veličine kapi, odnosno ispitivana smeša ostala je stabilna sa stanovišta medijane veličine kapi kao kriterijuma stabilnosti.",
publisher = "Vojnomedicinska akademija - Institut za naučne informacije, Beograd",
journal = "Vojnosanitetski pregled",
title = "Quality assessment of total parenteral nutrition admixtures by the use of fractional factorial design, Analiza kvaliteta smeša za totalnu parenteralnu ishranu primenom delimičnog faktorijalnog dizajna",
volume = "70",
number = "4",
pages = "374-379",
doi = "10.2298/VSP1304374M"
}

Mirković, D., Ibrić, S.,& Antunović, M.. (2013). Quality assessment of total parenteral nutrition admixtures by the use of fractional factorial design. in Vojnosanitetski pregled
Vojnomedicinska akademija - Institut za naučne informacije, Beograd., 70(4), 374-379.
https://doi.org/10.2298/VSP1304374M

Mirković D, Ibrić S, Antunović M. Quality assessment of total parenteral nutrition admixtures by the use of fractional factorial design. in Vojnosanitetski pregled. 2013;70(4):374-379.
doi:10.2298/VSP1304374M .

Mirković, Dušica, Ibrić, Svetlana, Antunović, Mirjana, "Quality assessment of total parenteral nutrition admixtures by the use of fractional factorial design" in Vojnosanitetski pregled, 70, no. 4 (2013):374-379,
https://doi.org/10.2298/VSP1304374M . .

TY  - JOUR
AU  - Đukić, Mirjana
AU  - Jovanović, Marina
AU  - Ninković, Milica
AU  - Stevanović, Ivana
AU  - Ćurčić, Marijana
AU  - Topić, Aleksandra
AU  - Vujanović, Dragana
AU  - Đurđević, Dragan
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1663
AB  - Introduction: Contact herbicide diquat (DQ), redox cycling compound, mediates its systemic toxicity throughout the enlarged production of free radicals. Target organs are liver and kidney in humans. To-date, the mechanism of DQ-induced neurotoxicity has not been rationalized. Objective: The objectives of the study were to examine the ability of DQ to induce oxidative stress (OS) and/or nitrosative stress (NS) upon intrastriatal (i.s.) administration and to investigate the role of nitric oxide (NOx) using NG-nitro-L-arginine methyl ester (L-NAME), a non-selective inhibitor of nitric oxide synthase (NOS) in the pretreatment of DQ i.s. administration. Material and Methods: The experiment was conducted on Wistar rats, randomly divided in experimental groups, receiving different treatments i.s. applied. Parameters of OS/NS such as: superoxide anion radical (O-2(center dot-)), superoxide dismutase (SOD), malondialdehyde (MDA) and nitrates (NO3-) were measured in the cortex (bilaterally), at 30th min, 24 hours and 7 days after the treatments. Results: Lethargy and high mortality rate were observed only in the DQ group (within 24 hours and 2-3 hours, respectively) after awakening from anesthesia. Markedly increased production of NOx and O-2(center dot-) along with elevated lipid peroxidation altogether contributed to DQ neurotoxicity. The most importantly, the L-NAME i.s. pretreatment protected treated animals from dying and diminished OS/NS response against DQ-induced neurotoxicity. Conclusion: The i.s. pretreatment with L-NAME resulted in neuroprotection against DQ neurotoxity, based on animal survival and reduced LPO in the cortex.
PB  - Inst Agricultural Medicine, Lublin
T2  - Annals of Agricultural and Environmental Medicine
T1  - Intrastriatal pre-treatment with L-NAME protects rats from diquat neurotoxcity
VL  - 19
IS  - 4
SP  - 666
EP  - 672
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1663
ER  - 

@article{
author = "Đukić, Mirjana and Jovanović, Marina and Ninković, Milica and Stevanović, Ivana and Ćurčić, Marijana and Topić, Aleksandra and Vujanović, Dragana and Đurđević, Dragan",
year = "2012",
abstract = "Introduction: Contact herbicide diquat (DQ), redox cycling compound, mediates its systemic toxicity throughout the enlarged production of free radicals. Target organs are liver and kidney in humans. To-date, the mechanism of DQ-induced neurotoxicity has not been rationalized. Objective: The objectives of the study were to examine the ability of DQ to induce oxidative stress (OS) and/or nitrosative stress (NS) upon intrastriatal (i.s.) administration and to investigate the role of nitric oxide (NOx) using NG-nitro-L-arginine methyl ester (L-NAME), a non-selective inhibitor of nitric oxide synthase (NOS) in the pretreatment of DQ i.s. administration. Material and Methods: The experiment was conducted on Wistar rats, randomly divided in experimental groups, receiving different treatments i.s. applied. Parameters of OS/NS such as: superoxide anion radical (O-2(center dot-)), superoxide dismutase (SOD), malondialdehyde (MDA) and nitrates (NO3-) were measured in the cortex (bilaterally), at 30th min, 24 hours and 7 days after the treatments. Results: Lethargy and high mortality rate were observed only in the DQ group (within 24 hours and 2-3 hours, respectively) after awakening from anesthesia. Markedly increased production of NOx and O-2(center dot-) along with elevated lipid peroxidation altogether contributed to DQ neurotoxicity. The most importantly, the L-NAME i.s. pretreatment protected treated animals from dying and diminished OS/NS response against DQ-induced neurotoxicity. Conclusion: The i.s. pretreatment with L-NAME resulted in neuroprotection against DQ neurotoxity, based on animal survival and reduced LPO in the cortex.",
publisher = "Inst Agricultural Medicine, Lublin",
journal = "Annals of Agricultural and Environmental Medicine",
title = "Intrastriatal pre-treatment with L-NAME protects rats from diquat neurotoxcity",
volume = "19",
number = "4",
pages = "666-672",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1663"
}

Đukić, M., Jovanović, M., Ninković, M., Stevanović, I., Ćurčić, M., Topić, A., Vujanović, D.,& Đurđević, D.. (2012). Intrastriatal pre-treatment with L-NAME protects rats from diquat neurotoxcity. in Annals of Agricultural and Environmental Medicine
Inst Agricultural Medicine, Lublin., 19(4), 666-672.
https://hdl.handle.net/21.15107/rcub_farfar_1663

Đukić M, Jovanović M, Ninković M, Stevanović I, Ćurčić M, Topić A, Vujanović D, Đurđević D. Intrastriatal pre-treatment with L-NAME protects rats from diquat neurotoxcity. in Annals of Agricultural and Environmental Medicine. 2012;19(4):666-672.
https://hdl.handle.net/21.15107/rcub_farfar_1663 .

Đukić, Mirjana, Jovanović, Marina, Ninković, Milica, Stevanović, Ivana, Ćurčić, Marijana, Topić, Aleksandra, Vujanović, Dragana, Đurđević, Dragan, "Intrastriatal pre-treatment with L-NAME protects rats from diquat neurotoxcity" in Annals of Agricultural and Environmental Medicine, 19, no. 4 (2012):666-672,
https://hdl.handle.net/21.15107/rcub_farfar_1663 .

TY  - JOUR
AU  - Đukić, Mirjana
AU  - Đurić, Ana
AU  - Tasić, Ljiljana
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1772
AB  - Abuse of psychoactive substances (PAS) is a major health and social problems, worldwide. The overall population is in direct daily contact with the PAS and thus, at risk of PAS abuse. Pharmacists have a unique and comprehensive knowledge about drugs and PAS, as well as, about the adverse effects of their inappropriate use (abuse). So far, regarding this problematic, the role of pharmacists, as health promoters, is mostly recognized in the field of pharmacotherapy and clinico-toxicological laboratory analysis. However, the role of pharmacists should be recognized more widely in the society, in the sense of prevention, advisory and educational role. According to the American model, in this area of work, pharmacists are responsible for prevention, education and assistance in overall health and social care system of addicts. Also, this paper gives an overview of the importance of institutional harmonization in the PAS domain abuse, at the city, region and state level. Inadequate cooperation at this level is a immense threat to society, and in the same time, an indicator of irresponsibility and neglect of the community regarding addiction related problems.
AB  - Zloupotreba psihoaktivnih supstanci (PAS) je veliki zdravstveno-društveni problem kod nas i u svetu. Sveukupna populacija je u neposrednom svakodnevnom kontaktu sa PAS i izložena je stalnom riziku od PAS. Farmaceuti imaju jedinstveno i sveobuhvatno znanje o bezbednoj i efikasnoj primeni lekova i PAS, kao i o neželjenim efektima do kojih dovodi njihova neadekvatna upotreba (zloupotreba). Uloga farmaceuta, za sada je uglavnom prepoznata u domenu farmakoterapije i kliničko-toksikoloških laboratorijskih analiza. Međutim, uloga farmaceuta kao promotera zdravlja bi trebalo da se prepozna šire u društvu, kada je reč o zloupotrebi i tretmanu zavisnika od PAS, kako u domenu prevencije, tako i tokom lečenja i kasnijoj fazi reintegracije lečenog pacijenta u društvenu zajednicu. Takođe, savetodavna i edukativna uloga farmaceuta u društvu, kod nas je oblast koja je u začetku, kada je reč o zloupotrebi PAS. Prema američkom modelu, odgovornosti farmaceuta u ovom domenu rada podeljene su u tri grupe: prevencija, edukacija i angažman u sveukupnom zdravstveno-socijalnom sistemu zbrinjavanja zavisnika. Takođe, u radu je dat osvrt na značaj institucionalne harmonizacije u domenu zloupotrebe PAS, na nivou grada, regiona i države. Neadekvatna saradnja na ovom nivou je velika pretnja društvu, a i u isto vreme, pokazatelj neodgovornosti i nebrige cele zajednice o problemu zavisnosti kod nas.
PB  - Savez farmaceutskih udruženja Srbije, Beograd
T2  - Arhiv za farmaciju
T1  - Abuse of psychoactive substances: The role of pharmacist
T1  - Zloupotreba psihoaktivnih supstanci - uloga farmaceuta
VL  - 62
IS  - 2
SP  - 179
EP  - 189
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1772
ER  - 

@article{
author = "Đukić, Mirjana and Đurić, Ana and Tasić, Ljiljana",
year = "2012",
abstract = "Abuse of psychoactive substances (PAS) is a major health and social problems, worldwide. The overall population is in direct daily contact with the PAS and thus, at risk of PAS abuse. Pharmacists have a unique and comprehensive knowledge about drugs and PAS, as well as, about the adverse effects of their inappropriate use (abuse). So far, regarding this problematic, the role of pharmacists, as health promoters, is mostly recognized in the field of pharmacotherapy and clinico-toxicological laboratory analysis. However, the role of pharmacists should be recognized more widely in the society, in the sense of prevention, advisory and educational role. According to the American model, in this area of work, pharmacists are responsible for prevention, education and assistance in overall health and social care system of addicts. Also, this paper gives an overview of the importance of institutional harmonization in the PAS domain abuse, at the city, region and state level. Inadequate cooperation at this level is a immense threat to society, and in the same time, an indicator of irresponsibility and neglect of the community regarding addiction related problems., Zloupotreba psihoaktivnih supstanci (PAS) je veliki zdravstveno-društveni problem kod nas i u svetu. Sveukupna populacija je u neposrednom svakodnevnom kontaktu sa PAS i izložena je stalnom riziku od PAS. Farmaceuti imaju jedinstveno i sveobuhvatno znanje o bezbednoj i efikasnoj primeni lekova i PAS, kao i o neželjenim efektima do kojih dovodi njihova neadekvatna upotreba (zloupotreba). Uloga farmaceuta, za sada je uglavnom prepoznata u domenu farmakoterapije i kliničko-toksikoloških laboratorijskih analiza. Međutim, uloga farmaceuta kao promotera zdravlja bi trebalo da se prepozna šire u društvu, kada je reč o zloupotrebi i tretmanu zavisnika od PAS, kako u domenu prevencije, tako i tokom lečenja i kasnijoj fazi reintegracije lečenog pacijenta u društvenu zajednicu. Takođe, savetodavna i edukativna uloga farmaceuta u društvu, kod nas je oblast koja je u začetku, kada je reč o zloupotrebi PAS. Prema američkom modelu, odgovornosti farmaceuta u ovom domenu rada podeljene su u tri grupe: prevencija, edukacija i angažman u sveukupnom zdravstveno-socijalnom sistemu zbrinjavanja zavisnika. Takođe, u radu je dat osvrt na značaj institucionalne harmonizacije u domenu zloupotrebe PAS, na nivou grada, regiona i države. Neadekvatna saradnja na ovom nivou je velika pretnja društvu, a i u isto vreme, pokazatelj neodgovornosti i nebrige cele zajednice o problemu zavisnosti kod nas.",
publisher = "Savez farmaceutskih udruženja Srbije, Beograd",
journal = "Arhiv za farmaciju",
title = "Abuse of psychoactive substances: The role of pharmacist, Zloupotreba psihoaktivnih supstanci - uloga farmaceuta",
volume = "62",
number = "2",
pages = "179-189",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1772"
}

Đukić, M., Đurić, A.,& Tasić, L.. (2012). Abuse of psychoactive substances: The role of pharmacist. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije, Beograd., 62(2), 179-189.
https://hdl.handle.net/21.15107/rcub_farfar_1772

Đukić M, Đurić A, Tasić L. Abuse of psychoactive substances: The role of pharmacist. in Arhiv za farmaciju. 2012;62(2):179-189.
https://hdl.handle.net/21.15107/rcub_farfar_1772 .

Đukić, Mirjana, Đurić, Ana, Tasić, Ljiljana, "Abuse of psychoactive substances: The role of pharmacist" in Arhiv za farmaciju, 62, no. 2 (2012):179-189,
https://hdl.handle.net/21.15107/rcub_farfar_1772 .

TY  - JOUR
AU  - Đukić, Mirjana
AU  - Ninković, Milica
AU  - Stevanović, Ivana
AU  - Ćurčić, Marijana
AU  - Ilić, Katarina
AU  - Đurđević, Dragan
AU  - Vujanović, Dragana
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1781
AB  - Most commonly observed central nervous system (CNS) effects induced by systemic toxicity of herbicide diquat (DQ) are general depression and lethargy. Generally, it is accepted that DQ exerts its toxicity through the production of superoxide anion radical (O2 ●-) during its redox metabolism in the presence of molecular oxygen, which further initiates radical chain reaction, contributing developing of oxidative stress (OS) as well. Mechanisms of DQ neurotoxic effect is not rationalized till now. The objective of the study was to examine whether OS contributes to DQ neurotxicity. For this purpose, male Wistar rats were intrastriataly (i.s.) treated with DQ and oxidative status parameters such as: superoxide anion radical (O2 ●-); nitrates (NO3 -), as a final metabolite of reactive nitogen species; malondialdehyde (MDA), an indicator of lipid peroxidation; activity of superoxide dismutase (SOD); glutathione peroxidase (GPx), and glutathione (GSH), were measured in the hippocampus at 30 minutes, 24 hours and 7 days post treatment. Noteworthy, mortality rate (30 - 40 %) was observed in the group of rats treated with DQ, within 2-3 hours after awakening from anesthesia. Additionally, lethargy was the only neurological symptom observed in that group. Analyzed parameters indicate that OS mediates DQ neurotxicity, which is documented with significant increase of lipid peroxidation.
AB  - Najčešće zapaženi efekti sistemskog trovanja herbicidom dikvatom (DK) na centralni nervni sistem (CNS) su opšta depresija i letargija. Opšte je prihvaćeno da se toksičnost DK ostvaruje posredstvom povećanog stvaranja superoksid anjon radikala (O2 ●-) tokom njegovog redoks metabolizma, u prisustvu molekularnog kiseonika, koji dalje inicira lančanu reakciju radikalskog tipa i razvoj oksidativnog stresa (OS). Do danas mehanizmi neurotoksičnog efekta DK nisu u potpunosti poznati. Cilj ove studije je bio da ispitamo da li OS posreduje u neurotoksičnosti indukovanoj DK. Eksperiment je sproveden na mužjacima Wistar pacova, intrastrijatalno tretiranih (i.s.) DK. Parametri oksidativnog statusa, kao što su: superoksid anjon radikal (O2 ●-), nitrati (NO3 -), kao finalni metaboliti reaktivnih vrsta azota; malondialdehid (MDA), indikator lipidne peroksidacije; aktivnost enzima; superoksid dizmutaze (SOD) i glutation peroksidaze (GPx); i glutation (GSH) mereni su u hipokampusu, 30 minuta, 24 sati i 7 dana posle tretmana. Stopa smrtnosti od 30 do 40 % ustanovljena je u grupi pacova tretiranih DK, tokom 2-3 sata od buđenja iz anestezije. Dodatno, pacovi ove grupe su pokazali neurološke simptome letargije. Značajno povećana lipidna peroksidacija pokazuju da OS posreduje u neurotoksičnom odgovoru indukovanom i.s. primenom DK.
PB  - Savez farmaceutskih udruženja Srbije, Beograd
T2  - Arhiv za farmaciju
T1  - Oxidative and nitrosative stress: Mediators of diquat neurotoxicity
T1  - Oksidativni i nitrozativni stres - medijatori neurotoksičnosti dikvata
VL  - 62
IS  - 5
SP  - 443
EP  - 460
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1781
ER  - 

@article{
author = "Đukić, Mirjana and Ninković, Milica and Stevanović, Ivana and Ćurčić, Marijana and Ilić, Katarina and Đurđević, Dragan and Vujanović, Dragana",
year = "2012",
abstract = "Most commonly observed central nervous system (CNS) effects induced by systemic toxicity of herbicide diquat (DQ) are general depression and lethargy. Generally, it is accepted that DQ exerts its toxicity through the production of superoxide anion radical (O2 ●-) during its redox metabolism in the presence of molecular oxygen, which further initiates radical chain reaction, contributing developing of oxidative stress (OS) as well. Mechanisms of DQ neurotoxic effect is not rationalized till now. The objective of the study was to examine whether OS contributes to DQ neurotxicity. For this purpose, male Wistar rats were intrastriataly (i.s.) treated with DQ and oxidative status parameters such as: superoxide anion radical (O2 ●-); nitrates (NO3 -), as a final metabolite of reactive nitogen species; malondialdehyde (MDA), an indicator of lipid peroxidation; activity of superoxide dismutase (SOD); glutathione peroxidase (GPx), and glutathione (GSH), were measured in the hippocampus at 30 minutes, 24 hours and 7 days post treatment. Noteworthy, mortality rate (30 - 40 %) was observed in the group of rats treated with DQ, within 2-3 hours after awakening from anesthesia. Additionally, lethargy was the only neurological symptom observed in that group. Analyzed parameters indicate that OS mediates DQ neurotxicity, which is documented with significant increase of lipid peroxidation., Najčešće zapaženi efekti sistemskog trovanja herbicidom dikvatom (DK) na centralni nervni sistem (CNS) su opšta depresija i letargija. Opšte je prihvaćeno da se toksičnost DK ostvaruje posredstvom povećanog stvaranja superoksid anjon radikala (O2 ●-) tokom njegovog redoks metabolizma, u prisustvu molekularnog kiseonika, koji dalje inicira lančanu reakciju radikalskog tipa i razvoj oksidativnog stresa (OS). Do danas mehanizmi neurotoksičnog efekta DK nisu u potpunosti poznati. Cilj ove studije je bio da ispitamo da li OS posreduje u neurotoksičnosti indukovanoj DK. Eksperiment je sproveden na mužjacima Wistar pacova, intrastrijatalno tretiranih (i.s.) DK. Parametri oksidativnog statusa, kao što su: superoksid anjon radikal (O2 ●-), nitrati (NO3 -), kao finalni metaboliti reaktivnih vrsta azota; malondialdehid (MDA), indikator lipidne peroksidacije; aktivnost enzima; superoksid dizmutaze (SOD) i glutation peroksidaze (GPx); i glutation (GSH) mereni su u hipokampusu, 30 minuta, 24 sati i 7 dana posle tretmana. Stopa smrtnosti od 30 do 40 % ustanovljena je u grupi pacova tretiranih DK, tokom 2-3 sata od buđenja iz anestezije. Dodatno, pacovi ove grupe su pokazali neurološke simptome letargije. Značajno povećana lipidna peroksidacija pokazuju da OS posreduje u neurotoksičnom odgovoru indukovanom i.s. primenom DK.",
publisher = "Savez farmaceutskih udruženja Srbije, Beograd",
journal = "Arhiv za farmaciju",
title = "Oxidative and nitrosative stress: Mediators of diquat neurotoxicity, Oksidativni i nitrozativni stres - medijatori neurotoksičnosti dikvata",
volume = "62",
number = "5",
pages = "443-460",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1781"
}

Đukić, M., Ninković, M., Stevanović, I., Ćurčić, M., Ilić, K., Đurđević, D.,& Vujanović, D.. (2012). Oxidative and nitrosative stress: Mediators of diquat neurotoxicity. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije, Beograd., 62(5), 443-460.
https://hdl.handle.net/21.15107/rcub_farfar_1781

Đukić M, Ninković M, Stevanović I, Ćurčić M, Ilić K, Đurđević D, Vujanović D. Oxidative and nitrosative stress: Mediators of diquat neurotoxicity. in Arhiv za farmaciju. 2012;62(5):443-460.
https://hdl.handle.net/21.15107/rcub_farfar_1781 .

Đukić, Mirjana, Ninković, Milica, Stevanović, Ivana, Ćurčić, Marijana, Ilić, Katarina, Đurđević, Dragan, Vujanović, Dragana, "Oxidative and nitrosative stress: Mediators of diquat neurotoxicity" in Arhiv za farmaciju, 62, no. 5 (2012):443-460,
https://hdl.handle.net/21.15107/rcub_farfar_1781 .

TY  - JOUR
AU  - Đukić, Mirjana
AU  - Jovanović, Marina
AU  - Ninković, Milica
AU  - Stevanović, Ivana
AU  - Đurđević, Dragan
AU  - Vasić, Una
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1792
AB  - In this study we examined if the response of the cortex against diquat (DQ), intrastriatally (i.s.) applied to Wistar rats, was mediated by oxidative/nitrosative stress (OS/NS). In particular, we were focused on the glutathione (GSH) antioxidative role, thus we applied i.s. glutathione reductase (GR) in the pre-treatment of DQ administration. Superoxide anion radical (O2 •-), nitrate (NO3 -), malondialdehyde (MDA) and superoxide dismutase (SOD), were measured in ipsi- and contra- lateral sides of the cortex, at 30 minutes, 24 hours and 7 days post treatment. The redox balance was not significantly changed in the cortex of sham operated and intact groups. Also, no differences were observed between the ipsi- and contra- lateral side of the cortex. Lethargy and mortality (30-40%) of the animals in the DQ group within 24 hrs, coincided with rapidly developed lipid peroxidation supported by OS/NS upon i.s. DQ administration. Strong redox potential of DQ probably resulted in a huge deprivation of molecular oxygen. The pretreatment with GR acted neuro-protectively, based on animal survival and absence of lethargy, although, lipid peroxidation was not developed in the GR+DQ group, OS was documented by a high concentration of O2 •- (within 24 hrs), descending and eventually inhibiting SOD activity (at 7 days).
AB  - U ovoj studiji smo ispitali da li je oksidativni/nitrosativni stres (OS/NS), uključen u odgovor korteksa Wistar pacova nakon intrastrijatalne (i.s.) izloženosti dikvatu (DK). Posebno smo ispitivali značaj antioksidativne uloge glutationa (GSH), zbog čega smo primenili glutation reduktazu (GR) u predtretmanu davanja DK. Superoksid anjon radikal (O2•¯), nitrati (NO3¯), malondialdehid (MDA) i superoksid dismutaza (SOD), su mereni u obostranom korteksu (ipsi- i kontrastrana), nakon 30 minuta, 24 sati i 7 dana od tretmana. Redoks balans se nije značajno promenio u korteksu lažno operisanih i netretiranih pacova. Takođe, ne postoji statistički značajna razlika između ipsi- i kontra- strane korteksa. Letargija i mortalitet (30-40%) kod životinja u DK grupi su uočene tokom 24 časa od i.s. trovanja DK, što se poklopilo sa naglim razvojem OS/NS i lipidne peroksidacije. Visok redoks potencijal DK verovatno rezultira opsežnim utroškom molekularnog kiseonika. Zaključeno je da je ostvaren neuroprotektivni učinak predtretmana sa GR, na osnovu preživljavanja životinja i odsustva letargije. Lipidna peroksidacija nije bila razvijena u grupi predtretiranoj sa GR ali je ipak izmerena visoka koncentracija O2•¯ (tokom 24 sata) koja zatim opada i na kraju 7. dana u potpunosti inhibira aktivnost SOD.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Acta veterinaria
T1  - Influence of glutathione reductase on diquat neurotoxcity assessed by oxidative/nitrosative stress in the cortex of intrastriatally treated rats
T1  - Uticaj glutation reduktaze na neurotoksičnost dikvata - ispitivan je oksidativni/nitrozativni stres u korteksu intrastrijatalno tretiranih pacova
VL  - 62
IS  - 5-6
SP  - 553
EP  - 568
DO  - 10.2298/AVB1206553D
ER  - 

@article{
author = "Đukić, Mirjana and Jovanović, Marina and Ninković, Milica and Stevanović, Ivana and Đurđević, Dragan and Vasić, Una",
year = "2012",
abstract = "In this study we examined if the response of the cortex against diquat (DQ), intrastriatally (i.s.) applied to Wistar rats, was mediated by oxidative/nitrosative stress (OS/NS). In particular, we were focused on the glutathione (GSH) antioxidative role, thus we applied i.s. glutathione reductase (GR) in the pre-treatment of DQ administration. Superoxide anion radical (O2 •-), nitrate (NO3 -), malondialdehyde (MDA) and superoxide dismutase (SOD), were measured in ipsi- and contra- lateral sides of the cortex, at 30 minutes, 24 hours and 7 days post treatment. The redox balance was not significantly changed in the cortex of sham operated and intact groups. Also, no differences were observed between the ipsi- and contra- lateral side of the cortex. Lethargy and mortality (30-40%) of the animals in the DQ group within 24 hrs, coincided with rapidly developed lipid peroxidation supported by OS/NS upon i.s. DQ administration. Strong redox potential of DQ probably resulted in a huge deprivation of molecular oxygen. The pretreatment with GR acted neuro-protectively, based on animal survival and absence of lethargy, although, lipid peroxidation was not developed in the GR+DQ group, OS was documented by a high concentration of O2 •- (within 24 hrs), descending and eventually inhibiting SOD activity (at 7 days)., U ovoj studiji smo ispitali da li je oksidativni/nitrosativni stres (OS/NS), uključen u odgovor korteksa Wistar pacova nakon intrastrijatalne (i.s.) izloženosti dikvatu (DK). Posebno smo ispitivali značaj antioksidativne uloge glutationa (GSH), zbog čega smo primenili glutation reduktazu (GR) u predtretmanu davanja DK. Superoksid anjon radikal (O2•¯), nitrati (NO3¯), malondialdehid (MDA) i superoksid dismutaza (SOD), su mereni u obostranom korteksu (ipsi- i kontrastrana), nakon 30 minuta, 24 sati i 7 dana od tretmana. Redoks balans se nije značajno promenio u korteksu lažno operisanih i netretiranih pacova. Takođe, ne postoji statistički značajna razlika između ipsi- i kontra- strane korteksa. Letargija i mortalitet (30-40%) kod životinja u DK grupi su uočene tokom 24 časa od i.s. trovanja DK, što se poklopilo sa naglim razvojem OS/NS i lipidne peroksidacije. Visok redoks potencijal DK verovatno rezultira opsežnim utroškom molekularnog kiseonika. Zaključeno je da je ostvaren neuroprotektivni učinak predtretmana sa GR, na osnovu preživljavanja životinja i odsustva letargije. Lipidna peroksidacija nije bila razvijena u grupi predtretiranoj sa GR ali je ipak izmerena visoka koncentracija O2•¯ (tokom 24 sata) koja zatim opada i na kraju 7. dana u potpunosti inhibira aktivnost SOD.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Acta veterinaria",
title = "Influence of glutathione reductase on diquat neurotoxcity assessed by oxidative/nitrosative stress in the cortex of intrastriatally treated rats, Uticaj glutation reduktaze na neurotoksičnost dikvata - ispitivan je oksidativni/nitrozativni stres u korteksu intrastrijatalno tretiranih pacova",
volume = "62",
number = "5-6",
pages = "553-568",
doi = "10.2298/AVB1206553D"
}

Đukić, M., Jovanović, M., Ninković, M., Stevanović, I., Đurđević, D.,& Vasić, U.. (2012). Influence of glutathione reductase on diquat neurotoxcity assessed by oxidative/nitrosative stress in the cortex of intrastriatally treated rats. in Acta veterinaria
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 62(5-6), 553-568.
https://doi.org/10.2298/AVB1206553D

Đukić M, Jovanović M, Ninković M, Stevanović I, Đurđević D, Vasić U. Influence of glutathione reductase on diquat neurotoxcity assessed by oxidative/nitrosative stress in the cortex of intrastriatally treated rats. in Acta veterinaria. 2012;62(5-6):553-568.
doi:10.2298/AVB1206553D .

Đukić, Mirjana, Jovanović, Marina, Ninković, Milica, Stevanović, Ivana, Đurđević, Dragan, Vasić, Una, "Influence of glutathione reductase on diquat neurotoxcity assessed by oxidative/nitrosative stress in the cortex of intrastriatally treated rats" in Acta veterinaria, 62, no. 5-6 (2012):553-568,
https://doi.org/10.2298/AVB1206553D . .

TY  - JOUR
AU  - Đukić, Mirjana
AU  - Ninković, Milica
AU  - Stevanović, Ivana
AU  - Ilić, Katarina
AU  - Đurđević, Dragan
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1778
AB  - Contact herbicide paraquat (DQ) is bipyridylium compound, which undergoes redox metabolism; hence enlarged in humans radical production mediated its toxicity. Target organs of systemic effect of PQ poisoning are lung and kidney. The mechanism of PQ-induced neurotoxicity is not elucidated till now. The objective of our study was to examine the role of nitric oxide (NOx) in PQ-induced neurotoxicity, primarily focusing on glutathione cycles [total glutathione content (GSH) and glutathione peroxidase (GPx) activity]. In order to investigate the role of NOx in oxidative stress (OS) and/or nitrosative stress (NS) response to PQ neurotoxicity, we used NG-nitro-L-arginine methyl ester (L-NAME), a non-selective inhibitor of nitric oxide synthase (NOS) in the pretreatment of PQ administration. Study was conducted on Wistar rats randomly divided in groups (n=8 for controls and n=24 for experimental groups) depending on the applied treatments. The tested compounds were intrastriatally (i.s.) administered. Measuring of GSH content and GPx activity was performed at 30 min, 24 hours and 7 days after treatments. Parkinsonism’s like symptoms were observed only in the group of rats treated with PQ. The L-NAME protected animals from PQ-induced neurotoxicity, which could be concluded from the absence of Parkinsonism’s like symptoms and reduced OS/NS response in the striatum of rats pretreated with L-NAME.
AB  - Kontaktni herbicid parakvat (PK) je dipiridinsko jedinjenje, koje podleže redoks metabolizmu i svoju toksičnost ispoljava posredstvom povećanog stvaranja slobodnih radikala. Ciljni organi sistemskog toksičnog efekta PK kod čoveka su pluća i bubrezi. Mehanizam PKindukovane neurotoksičnosti do sada još uvek nije u potpunosti rasvetljen. Cilj našeg rada bio je da se ispita uloga azot oksida (NOx) u neurotoksičnosti PK, sa posebnim osvrtom na glutationski ciklus [glutation (GSH) i enzim glutation peroksidazu (GPx)]. U cilju da se istraži uloga NOx u oksidativnom stresu (OS) i / ili nitrosativnom stresu (NS), kao odgovoru na neurotoksičnost PK. U predtretmanu parakvatom koristili smo NG-nitro- L-arginin metil estar (L-NAME), neselektivni inhibitor azot oksid sintetaze (NOS), aplikovan je pre davanja PQ . Studija je sprovedena na pacovima Wistar soja nasumice podeljenim u grupe (n = 8 za kontrolnu grupu i n = 24 za eksperimentalne grupe) u zavisnosti od tretmana. Testirana jedinjenja su intrastrijatalno (i.s.) aplikovana. Merenje sadržaja GSH i aktivnosti GPx izvršena su 30 min, 24 sata i 7 dana posle tretmana. Parkinsonizam je kao simptom primećen samo u grupi pacova tretiranih PK - om. L-NAME je ispoljio zaštitni efekat kod životinja kod kojih je i.s. davan PK, što bi se moglo zaključiti na osnovu odsustva simptoma parkinsonizma i smanjenog OS/NS odgovora u strijatumu u grupi pretretiranoj sa L-NAME.
PB  - Savez farmaceutskih udruženja Srbije, Beograd
T2  - Arhiv za farmaciju
T1  - The effect of pre-treatment with l-name on glutathione and glutathione peroxidase in parquat-induced neurotoxicity in rat striatum
T1  - Efekat predtretmana sa l-name na glutation i glutation peroksidazu u strijatumu pacova na neurotoksičnost izazvanu parakvatom
VL  - 62
IS  - 3
SP  - 237
EP  - 251
UR  - https://hdl.handle.net/21.15107/rcub_farfar_1778
ER  - 

@article{
author = "Đukić, Mirjana and Ninković, Milica and Stevanović, Ivana and Ilić, Katarina and Đurđević, Dragan",
year = "2012",
abstract = "Contact herbicide paraquat (DQ) is bipyridylium compound, which undergoes redox metabolism; hence enlarged in humans radical production mediated its toxicity. Target organs of systemic effect of PQ poisoning are lung and kidney. The mechanism of PQ-induced neurotoxicity is not elucidated till now. The objective of our study was to examine the role of nitric oxide (NOx) in PQ-induced neurotoxicity, primarily focusing on glutathione cycles [total glutathione content (GSH) and glutathione peroxidase (GPx) activity]. In order to investigate the role of NOx in oxidative stress (OS) and/or nitrosative stress (NS) response to PQ neurotoxicity, we used NG-nitro-L-arginine methyl ester (L-NAME), a non-selective inhibitor of nitric oxide synthase (NOS) in the pretreatment of PQ administration. Study was conducted on Wistar rats randomly divided in groups (n=8 for controls and n=24 for experimental groups) depending on the applied treatments. The tested compounds were intrastriatally (i.s.) administered. Measuring of GSH content and GPx activity was performed at 30 min, 24 hours and 7 days after treatments. Parkinsonism’s like symptoms were observed only in the group of rats treated with PQ. The L-NAME protected animals from PQ-induced neurotoxicity, which could be concluded from the absence of Parkinsonism’s like symptoms and reduced OS/NS response in the striatum of rats pretreated with L-NAME., Kontaktni herbicid parakvat (PK) je dipiridinsko jedinjenje, koje podleže redoks metabolizmu i svoju toksičnost ispoljava posredstvom povećanog stvaranja slobodnih radikala. Ciljni organi sistemskog toksičnog efekta PK kod čoveka su pluća i bubrezi. Mehanizam PKindukovane neurotoksičnosti do sada još uvek nije u potpunosti rasvetljen. Cilj našeg rada bio je da se ispita uloga azot oksida (NOx) u neurotoksičnosti PK, sa posebnim osvrtom na glutationski ciklus [glutation (GSH) i enzim glutation peroksidazu (GPx)]. U cilju da se istraži uloga NOx u oksidativnom stresu (OS) i / ili nitrosativnom stresu (NS), kao odgovoru na neurotoksičnost PK. U predtretmanu parakvatom koristili smo NG-nitro- L-arginin metil estar (L-NAME), neselektivni inhibitor azot oksid sintetaze (NOS), aplikovan je pre davanja PQ . Studija je sprovedena na pacovima Wistar soja nasumice podeljenim u grupe (n = 8 za kontrolnu grupu i n = 24 za eksperimentalne grupe) u zavisnosti od tretmana. Testirana jedinjenja su intrastrijatalno (i.s.) aplikovana. Merenje sadržaja GSH i aktivnosti GPx izvršena su 30 min, 24 sata i 7 dana posle tretmana. Parkinsonizam je kao simptom primećen samo u grupi pacova tretiranih PK - om. L-NAME je ispoljio zaštitni efekat kod životinja kod kojih je i.s. davan PK, što bi se moglo zaključiti na osnovu odsustva simptoma parkinsonizma i smanjenog OS/NS odgovora u strijatumu u grupi pretretiranoj sa L-NAME.",
publisher = "Savez farmaceutskih udruženja Srbije, Beograd",
journal = "Arhiv za farmaciju",
title = "The effect of pre-treatment with l-name on glutathione and glutathione peroxidase in parquat-induced neurotoxicity in rat striatum, Efekat predtretmana sa l-name na glutation i glutation peroksidazu u strijatumu pacova na neurotoksičnost izazvanu parakvatom",
volume = "62",
number = "3",
pages = "237-251",
url = "https://hdl.handle.net/21.15107/rcub_farfar_1778"
}

Đukić, M., Ninković, M., Stevanović, I., Ilić, K.,& Đurđević, D.. (2012). The effect of pre-treatment with l-name on glutathione and glutathione peroxidase in parquat-induced neurotoxicity in rat striatum. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije, Beograd., 62(3), 237-251.
https://hdl.handle.net/21.15107/rcub_farfar_1778

Đukić M, Ninković M, Stevanović I, Ilić K, Đurđević D. The effect of pre-treatment with l-name on glutathione and glutathione peroxidase in parquat-induced neurotoxicity in rat striatum. in Arhiv za farmaciju. 2012;62(3):237-251.
https://hdl.handle.net/21.15107/rcub_farfar_1778 .

Đukić, Mirjana, Ninković, Milica, Stevanović, Ivana, Ilić, Katarina, Đurđević, Dragan, "The effect of pre-treatment with l-name on glutathione and glutathione peroxidase in parquat-induced neurotoxicity in rat striatum" in Arhiv za farmaciju, 62, no. 3 (2012):237-251,
https://hdl.handle.net/21.15107/rcub_farfar_1778 .

TY  - JOUR
AU  - Đukić, Mirjana
AU  - Jovanović, Marina
AU  - Ninković, Milica
AU  - Stevanović, Ivana
AU  - Ilić, Katarina
AU  - Ćurčić, Marijana
AU  - Vekić, Jelena
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1653
AB  - Paraquat (PQ), a widely used herbicide is a well-known free radical producing agent. The mechanistic pathways of PQ neurotoxicity were examined by assessing oxidative/nitrosative stress markers. Focus was on the role of glutathione (GSH) cycle and to examine whether the pre-treatment with enzyme glutathione reductase (GR) could protect the vulnerable brain regions (VBRs) against harmful oxidative effect of PQ. The study was conducted on Wistar rats, randomly divided in five groups: intact-control group, (n = 8) and four experimental groups (n = 24). All tested compounds were administered intrastriatally (i.s.) in one single dose. The following parameters of oxidative status were measured in the striatum, hippocampus and cortex, at 30 min, 24 h and 7 days post treatment: superoxide anion radical (O-2(center dot-)), nitrate (NO3-). malondialdehyde (MDA), superoxide dismutase (SOD), total GSH (tGSH) and its oxidized, disulfide form (GSSG) and glutathione peroxidase (GPx). Results obtained from the intact and the sham operated groups were not statistically different, confirming that invasive i.s. route of administration would not influence the reliability of results. Also, similar pattern of changes were observed between ipsi- and contra- lateral side of examined VBRs, indicating rapid spatial spreading of oxidative stress. Mortality of the animals (10%), within 24 h, along with symptoms of Parkinsonism, after awakening from anesthesia for 2-3 h, were observed in the PQ group, only. Increased levels of O2(center dot-), NO3- and MDA, increased ratio of GSSG/GSH and considerably high activity of GPx were measured at 30 min after the treatment. Cytotoxic effect of PQ was documented by drastic drop of all measured parameters and extremely high peak of the ratio GSSG/GSH at 24th hrs after the PQ i.s. injection. In the GR + PQ group, markedly low activity of GPx and low content of NO3- (in striatum and cortex) were measured during whole experiment, while increase value was observed only for O-2(center dot-), at 7th days. We concluded that oxidative/nitrosative stress and excitotoxicity are the most important events since the early stage of PQ induced neurotoxicity. Based on the ratio GSSG/GSH, the oxidation of GSH to GSSG is probably dominant way of GHS depletion and main reason for reduced antioxidative defense against PQ harmful oxidative effect. The GR pre-treatment resulted in the absence of Parkinson's disease-like symptoms and mortality of the rats. Additionally, oxidative/nitrosative stress did not developed, as well as almost diminished metabolism of the VBRs at 24th hours (as has been documented in the PQgroup) did not occurred in the GR + PQ suggesting a neuroprotective role for the GR in PQ induced neurotoxicity.
PB  - Elsevier Ireland Ltd, Clare
T2  - Chemico-Biological Interactions
T1  - Protective role of glutathione reductase in paraquat induced neurotoxicity
VL  - 199
IS  - 2
SP  - 74
EP  - 86
DO  - 10.1016/j.cbi.2012.05.008
ER  - 

@article{
author = "Đukić, Mirjana and Jovanović, Marina and Ninković, Milica and Stevanović, Ivana and Ilić, Katarina and Ćurčić, Marijana and Vekić, Jelena",
year = "2012",
abstract = "Paraquat (PQ), a widely used herbicide is a well-known free radical producing agent. The mechanistic pathways of PQ neurotoxicity were examined by assessing oxidative/nitrosative stress markers. Focus was on the role of glutathione (GSH) cycle and to examine whether the pre-treatment with enzyme glutathione reductase (GR) could protect the vulnerable brain regions (VBRs) against harmful oxidative effect of PQ. The study was conducted on Wistar rats, randomly divided in five groups: intact-control group, (n = 8) and four experimental groups (n = 24). All tested compounds were administered intrastriatally (i.s.) in one single dose. The following parameters of oxidative status were measured in the striatum, hippocampus and cortex, at 30 min, 24 h and 7 days post treatment: superoxide anion radical (O-2(center dot-)), nitrate (NO3-). malondialdehyde (MDA), superoxide dismutase (SOD), total GSH (tGSH) and its oxidized, disulfide form (GSSG) and glutathione peroxidase (GPx). Results obtained from the intact and the sham operated groups were not statistically different, confirming that invasive i.s. route of administration would not influence the reliability of results. Also, similar pattern of changes were observed between ipsi- and contra- lateral side of examined VBRs, indicating rapid spatial spreading of oxidative stress. Mortality of the animals (10%), within 24 h, along with symptoms of Parkinsonism, after awakening from anesthesia for 2-3 h, were observed in the PQ group, only. Increased levels of O2(center dot-), NO3- and MDA, increased ratio of GSSG/GSH and considerably high activity of GPx were measured at 30 min after the treatment. Cytotoxic effect of PQ was documented by drastic drop of all measured parameters and extremely high peak of the ratio GSSG/GSH at 24th hrs after the PQ i.s. injection. In the GR + PQ group, markedly low activity of GPx and low content of NO3- (in striatum and cortex) were measured during whole experiment, while increase value was observed only for O-2(center dot-), at 7th days. We concluded that oxidative/nitrosative stress and excitotoxicity are the most important events since the early stage of PQ induced neurotoxicity. Based on the ratio GSSG/GSH, the oxidation of GSH to GSSG is probably dominant way of GHS depletion and main reason for reduced antioxidative defense against PQ harmful oxidative effect. The GR pre-treatment resulted in the absence of Parkinson's disease-like symptoms and mortality of the rats. Additionally, oxidative/nitrosative stress did not developed, as well as almost diminished metabolism of the VBRs at 24th hours (as has been documented in the PQgroup) did not occurred in the GR + PQ suggesting a neuroprotective role for the GR in PQ induced neurotoxicity.",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Chemico-Biological Interactions",
title = "Protective role of glutathione reductase in paraquat induced neurotoxicity",
volume = "199",
number = "2",
pages = "74-86",
doi = "10.1016/j.cbi.2012.05.008"
}

Đukić, M., Jovanović, M., Ninković, M., Stevanović, I., Ilić, K., Ćurčić, M.,& Vekić, J.. (2012). Protective role of glutathione reductase in paraquat induced neurotoxicity. in Chemico-Biological Interactions
Elsevier Ireland Ltd, Clare., 199(2), 74-86.
https://doi.org/10.1016/j.cbi.2012.05.008

Đukić M, Jovanović M, Ninković M, Stevanović I, Ilić K, Ćurčić M, Vekić J. Protective role of glutathione reductase in paraquat induced neurotoxicity. in Chemico-Biological Interactions. 2012;199(2):74-86.
doi:10.1016/j.cbi.2012.05.008 .

Đukić, Mirjana, Jovanović, Marina, Ninković, Milica, Stevanović, Ivana, Ilić, Katarina, Ćurčić, Marijana, Vekić, Jelena, "Protective role of glutathione reductase in paraquat induced neurotoxicity" in Chemico-Biological Interactions, 199, no. 2 (2012):74-86,
https://doi.org/10.1016/j.cbi.2012.05.008 . .