Faculty of Medicine University of Niš Internal Scientific Project No. 15

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Faculty of Medicine University of Niš Internal Scientific Project No. 15

Authors

Publications

In vitro cytotoxic and anti-inflammatory activity of Bruckenthalia spiculifolia extracts and fractions

Pavlović, Dragana; Stojanović, Nikola; Zlatković, Bojan; Radulović, Niko; Kovačević, Nada

(Savez farmaceutskih udruženja Srbije (SFUS), 2022) 

TY  - CONF
AU  - Pavlović, Dragana
AU  - Stojanović, Nikola
AU  - Zlatković, Bojan
AU  - Radulović, Niko
AU  - Kovačević, Nada
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4579
AB  - According to our previous studies, Bruckenthalia spiculifolia Rchb. (Ericaceae) possess
strong antioxidant activity and hydrogel loaded with 2% ethanolic extract from its flowers
and leaves is a potential southing and wound healing agent (1). Ethanolic extracts of leaves,
flowers, and herba, alongside fractions obtained by systematic solvent extraction method
with solvents of the increasing polarity of liquid-liquid fractionation of areal parts of B.
spiculifolia, were prepared. To estimate in vitro anti-inflammatory activities of eight different
extracts and fractions, isolated rat peritoneal macrophages were employed. MTT test was
used for the cytotoxicity determination, while the anti-inflammatory potential
lipopolysaccharide-induced NO production model was utilized (2). Experimental procedures
with animals complied with the rules of the European Union Normative. Resident rat
peritoneal macrophages were harvested by peritoneal lavage and used for both procedures.
Phytochemical analysis was done by RP-HPLC coupled with DAD detection. Tested samples
statistically significantly decreased macrophage ability to metabolize MTT, mainly in a
concentration-dependent manner. All of them also significantly inhibited NO production
from macrophages stimulated with lipopolysaccharide, which can be related to either their
cytotoxic or anti-inflammatory activity. Quantified polar compounds (especially quercetin-3-
O-glucoside) could be associated with better antioxidant and anti-inflammatory effects, as
well as with a lower cytotoxic potential. Indeed, fractions obtained with ethyl acetate and
water, but also ethanol extract obtained after lipids removal with petroleum ether, showed
the most promising effects. These results point out that B. spiculifolia is worth further
investigation in order to detect new naturally occurring anti-inflammatory agents.
AB  - Prema našim prethodnim istraživanjima, Bruckenthalia spiculifolia Rchb. (Ericaceae)
poseduje jaku antioksidativnu aktivnost, a hidrogel sa 2% etanolnog ekstrakta cveta i lista
ove biljne vrste pospešuje zarastanje rana (1). Pripremljeni su etanolni ekstrakti listova,
cvetova i nadzemnog dela biljne vrste B. spiculifolia, kao i frakcije dobijene sistematskom
ekstrakcijom rastvaračima rastuć eg polariteta tečno-tečno frakcionisanjem nadzemnih
delova biljke. Za procenu lokalne antiinflamatorne aktivnosti ukupno osam različitih
ekstrakata i frakcija korišćeni su izolovani peritonealni makrofagi pacova. MTT test je služio
za određivanje citotoksičnosti, a model produkcije NO izazvane lipopolisaharidom za in vitro
kvantifikaciju antiinflamatornog potencijala (2). Eksperimentalne procedure sa životinjama
su usklađeni sa zahtevima Evropske unije. Peritonealni makrofagi pacova su sakupljeni
peritonealnim ispiranjem i korišćeni za obe procedure. Fitohemijska analiza je izvršna
pomoć u RP-HPLC sa DAD detekcijom. Testirani uzorci su statistički značajno smanjili
sposobnost makrofaga da metabolišu MTT i to uglavnom na dozno-zavisan način. Svi oni su
takođe značajno inhibirali proizvodnju NO iz makrofaga stimulisanih lipopolisaharidom, što
se može dovesti u vezu sa njihovom citotoksičnom ili antiinflamatornom aktivnošć u.
Kvantifikovana polarna jedinjenja (naročito kvercetin-3-O-glukozid) mogu biti povezana sa
boljim antioksidativnim i antiinflamatornim efektima, kao i sa nižim citotoksičnim
potencijalom. Zaista, frakcije dobijene sa etil acetatom i vodom, ali i etanolni ekstrakt dobijen
nakon uklanjanja lipida petroletrom, su pokazale najbolje efekte. Izloženi rezultati ukazuju
na to da vrstu B. spiculifolia treba dalje ispitati u cilju pronalaženja novih prirodnih
antiinflamatornih agensa.
PB  - Savez farmaceutskih udruženja Srbije (SFUS)
C3  - Arhiv za farmaciju
T1  - In vitro cytotoxic and anti-inflammatory activity of Bruckenthalia spiculifolia extracts and fractions
T1  - In vitro citotoksična i antiinflamatorna aktivnost ekstrakata i frakcija Bruckenthalia spiculifolia
VL  - 72
IS  - 4 suplement
SP  - S453
EP  - S454
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4579
ER  - 
@conference{
author = "Pavlović, Dragana and Stojanović, Nikola and Zlatković, Bojan and Radulović, Niko and Kovačević, Nada",
year = "2022",
abstract = "According to our previous studies, Bruckenthalia spiculifolia Rchb. (Ericaceae) possess
strong antioxidant activity and hydrogel loaded with 2% ethanolic extract from its flowers
and leaves is a potential southing and wound healing agent (1). Ethanolic extracts of leaves,
flowers, and herba, alongside fractions obtained by systematic solvent extraction method
with solvents of the increasing polarity of liquid-liquid fractionation of areal parts of B.
spiculifolia, were prepared. To estimate in vitro anti-inflammatory activities of eight different
extracts and fractions, isolated rat peritoneal macrophages were employed. MTT test was
used for the cytotoxicity determination, while the anti-inflammatory potential
lipopolysaccharide-induced NO production model was utilized (2). Experimental procedures
with animals complied with the rules of the European Union Normative. Resident rat
peritoneal macrophages were harvested by peritoneal lavage and used for both procedures.
Phytochemical analysis was done by RP-HPLC coupled with DAD detection. Tested samples
statistically significantly decreased macrophage ability to metabolize MTT, mainly in a
concentration-dependent manner. All of them also significantly inhibited NO production
from macrophages stimulated with lipopolysaccharide, which can be related to either their
cytotoxic or anti-inflammatory activity. Quantified polar compounds (especially quercetin-3-
O-glucoside) could be associated with better antioxidant and anti-inflammatory effects, as
well as with a lower cytotoxic potential. Indeed, fractions obtained with ethyl acetate and
water, but also ethanol extract obtained after lipids removal with petroleum ether, showed
the most promising effects. These results point out that B. spiculifolia is worth further
investigation in order to detect new naturally occurring anti-inflammatory agents., Prema našim prethodnim istraživanjima, Bruckenthalia spiculifolia Rchb. (Ericaceae)
poseduje jaku antioksidativnu aktivnost, a hidrogel sa 2% etanolnog ekstrakta cveta i lista
ove biljne vrste pospešuje zarastanje rana (1). Pripremljeni su etanolni ekstrakti listova,
cvetova i nadzemnog dela biljne vrste B. spiculifolia, kao i frakcije dobijene sistematskom
ekstrakcijom rastvaračima rastuć eg polariteta tečno-tečno frakcionisanjem nadzemnih
delova biljke. Za procenu lokalne antiinflamatorne aktivnosti ukupno osam različitih
ekstrakata i frakcija korišćeni su izolovani peritonealni makrofagi pacova. MTT test je služio
za određivanje citotoksičnosti, a model produkcije NO izazvane lipopolisaharidom za in vitro
kvantifikaciju antiinflamatornog potencijala (2). Eksperimentalne procedure sa životinjama
su usklađeni sa zahtevima Evropske unije. Peritonealni makrofagi pacova su sakupljeni
peritonealnim ispiranjem i korišćeni za obe procedure. Fitohemijska analiza je izvršna
pomoć u RP-HPLC sa DAD detekcijom. Testirani uzorci su statistički značajno smanjili
sposobnost makrofaga da metabolišu MTT i to uglavnom na dozno-zavisan način. Svi oni su
takođe značajno inhibirali proizvodnju NO iz makrofaga stimulisanih lipopolisaharidom, što
se može dovesti u vezu sa njihovom citotoksičnom ili antiinflamatornom aktivnošć u.
Kvantifikovana polarna jedinjenja (naročito kvercetin-3-O-glukozid) mogu biti povezana sa
boljim antioksidativnim i antiinflamatornim efektima, kao i sa nižim citotoksičnim
potencijalom. Zaista, frakcije dobijene sa etil acetatom i vodom, ali i etanolni ekstrakt dobijen
nakon uklanjanja lipida petroletrom, su pokazale najbolje efekte. Izloženi rezultati ukazuju
na to da vrstu B. spiculifolia treba dalje ispitati u cilju pronalaženja novih prirodnih
antiinflamatornih agensa.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "In vitro cytotoxic and anti-inflammatory activity of Bruckenthalia spiculifolia extracts and fractions, In vitro citotoksična i antiinflamatorna aktivnost ekstrakata i frakcija Bruckenthalia spiculifolia",
volume = "72",
number = "4 suplement",
pages = "S453-S454",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4579"
}
Pavlović, D., Stojanović, N., Zlatković, B., Radulović, N.,& Kovačević, N.. (2022). In vitro cytotoxic and anti-inflammatory activity of Bruckenthalia spiculifolia extracts and fractions. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije (SFUS)., 72(4 suplement), S453-S454.
https://hdl.handle.net/21.15107/rcub_farfar_4579
Pavlović D, Stojanović N, Zlatković B, Radulović N, Kovačević N. In vitro cytotoxic and anti-inflammatory activity of Bruckenthalia spiculifolia extracts and fractions. in Arhiv za farmaciju. 2022;72(4 suplement):S453-S454.
https://hdl.handle.net/21.15107/rcub_farfar_4579 .
Pavlović, Dragana, Stojanović, Nikola, Zlatković, Bojan, Radulović, Niko, Kovačević, Nada, "In vitro cytotoxic and anti-inflammatory activity of Bruckenthalia spiculifolia extracts and fractions" in Arhiv za farmaciju, 72, no. 4 suplement (2022):S453-S454,
https://hdl.handle.net/21.15107/rcub_farfar_4579 .