TY  - JOUR
AU  - Ušjak, Dušan
AU  - Novović, Katarina
AU  - Filipić, Brankica
AU  - Kojić, Milan
AU  - Filipović, Nenad
AU  - Stevanović, Magdalena
AU  - Milenković, Marina
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4177
AB  - Aims: To investigate the synergistic activity of colistin and selenium nanoparticles (SeNPs) against pandrug-resistant (PDR) Ac. baumannii. Methods and Results: Chequerboard and time-kill assays were employed to ex- plore the potential synergistic interactions between colistin and SeNPs against Ac. baumannii isolates (8), previously determined as colistin-resistant (MIC range 16– 256 μg ml−1 ). Also, whole-genome sequencing (WGS) and gene expression analyses were used to elucidate the mechanisms of colistin resistance. Exceptionally strong synergistic activity (FICI range 0.004–0.035) of colistin and SeNPs against colistin- resistant isolates was revealed. Colistin (0.5 or 1 μg ml −1 ) used in combination with SeNPs (0.5 μg ml−1 ) was able to reduce initial inoculum during the first 4 h of incuba- tion, in contrast to colistin (0.5, 1 or 2 μg ml−1 ) alone. Conclusions: These findings propose colistin/SeNPs combination as a new option to fight PDR Ac. baumannii, the therapeutic possibilities of which should be proved in future in vivo studies. Significance and Impact of Study: Here we present the first evidence of synergy between colistin and selenium compounds against bacteria in general. Also, WGS and gene expression analyses provide some new insights into Ac. baumannii colistin resistance mechanisms.
PB  - John Wiley and Sons Inc
T2  - Journal of Applied Microbiology
T1  - In vitro colistin susceptibility of pandrug-resistant Ac. baumannii is restored in the presence of selenium nanoparticles
VL  - 133
IS  - 3
SP  - 1197
SP  - 1197
EP  - 1206
DO  - 10.1111/jam.15638
ER  - 

@article{
author = "Ušjak, Dušan and Novović, Katarina and Filipić, Brankica and Kojić, Milan and Filipović, Nenad and Stevanović, Magdalena and Milenković, Marina",
year = "2022",
abstract = "Aims: To investigate the synergistic activity of colistin and selenium nanoparticles (SeNPs) against pandrug-resistant (PDR) Ac. baumannii. Methods and Results: Chequerboard and time-kill assays were employed to ex- plore the potential synergistic interactions between colistin and SeNPs against Ac. baumannii isolates (8), previously determined as colistin-resistant (MIC range 16– 256 μg ml−1 ). Also, whole-genome sequencing (WGS) and gene expression analyses were used to elucidate the mechanisms of colistin resistance. Exceptionally strong synergistic activity (FICI range 0.004–0.035) of colistin and SeNPs against colistin- resistant isolates was revealed. Colistin (0.5 or 1 μg ml −1 ) used in combination with SeNPs (0.5 μg ml−1 ) was able to reduce initial inoculum during the first 4 h of incuba- tion, in contrast to colistin (0.5, 1 or 2 μg ml−1 ) alone. Conclusions: These findings propose colistin/SeNPs combination as a new option to fight PDR Ac. baumannii, the therapeutic possibilities of which should be proved in future in vivo studies. Significance and Impact of Study: Here we present the first evidence of synergy between colistin and selenium compounds against bacteria in general. Also, WGS and gene expression analyses provide some new insights into Ac. baumannii colistin resistance mechanisms.",
publisher = "John Wiley and Sons Inc",
journal = "Journal of Applied Microbiology",
title = "In vitro colistin susceptibility of pandrug-resistant Ac. baumannii is restored in the presence of selenium nanoparticles",
volume = "133",
number = "3",
pages = "1197-1197-1206",
doi = "10.1111/jam.15638"
}

Ušjak, D., Novović, K., Filipić, B., Kojić, M., Filipović, N., Stevanović, M.,& Milenković, M.. (2022). In vitro colistin susceptibility of pandrug-resistant Ac. baumannii is restored in the presence of selenium nanoparticles. in Journal of Applied Microbiology
John Wiley and Sons Inc., 133(3), 1197-1206.
https://doi.org/10.1111/jam.15638

Ušjak D, Novović K, Filipić B, Kojić M, Filipović N, Stevanović M, Milenković M. In vitro colistin susceptibility of pandrug-resistant Ac. baumannii is restored in the presence of selenium nanoparticles. in Journal of Applied Microbiology. 2022;133(3):1197-1206.
doi:10.1111/jam.15638 .

Ušjak, Dušan, Novović, Katarina, Filipić, Brankica, Kojić, Milan, Filipović, Nenad, Stevanović, Magdalena, Milenković, Marina, "In vitro colistin susceptibility of pandrug-resistant Ac. baumannii is restored in the presence of selenium nanoparticles" in Journal of Applied Microbiology, 133, no. 3 (2022):1197-1206,
https://doi.org/10.1111/jam.15638 . .

TY  - JOUR
AU  - Mirković, Nemanja
AU  - Obradović, Mina
AU  - O’Connor, Paula M.
AU  - Filipić, Brankica
AU  - Jovčić, Branko
AU  - Cotter, Paul D.
AU  - Kojić, Milan
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3932
AB  - Screening for producers of potent antimicrobial peptides, resulted in the isolation of Bacillus cereus BGNM1 with strong antimicrobial activity against Listeria monocytogenes. Genome sequence analysis revealed that BGNM1 contains the gene cluster associated with the production of the lantibiotic, thusin, previously identified in B. thuringiensis. Purification of the antimicrobial activity confirmed that strain BGMN1 produces thusin. Both thusin sensitive and resistant strains were detected among clinical isolates of Streptococcus agalactiae. Random mutagenesis of a thusin sensitive strain, S. agalactiae B782, was performed in an attempt to identify the receptor protein for thusin. Three independent thusin resistant mutants were selected and their complete genomes sequenced. Comparative sequence analysis of these mutants with the WT strain revealed that duplication of a region encoding a 79 amino acids repeat in a C-protein α-antigen was a common difference, suggesting it to be responsible for increased resistance to thusin. Since induced thusin resistant mutants showed higher level of resistance than the naturally resistant B761 strain, complete genome sequencing of strain B761 was performed to check the integrity of the C-protein α-antigen-encoding gene. This analysis revealed that this gene is deleted in B761, providing further evidence that this protein promotes interaction of the thusin with receptor.
PB  - Springer Nature
T2  - Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology
T1  - C-protein α-antigen modulates the lantibiotic thusin resistance in Streptococcus agalactiae
VL  - 114
SP  - 1595
EP  - 1607
DO  - 10.1007/s10482-021-01626-3
ER  - 

@article{
author = "Mirković, Nemanja and Obradović, Mina and O’Connor, Paula M. and Filipić, Brankica and Jovčić, Branko and Cotter, Paul D. and Kojić, Milan",
year = "2021",
abstract = "Screening for producers of potent antimicrobial peptides, resulted in the isolation of Bacillus cereus BGNM1 with strong antimicrobial activity against Listeria monocytogenes. Genome sequence analysis revealed that BGNM1 contains the gene cluster associated with the production of the lantibiotic, thusin, previously identified in B. thuringiensis. Purification of the antimicrobial activity confirmed that strain BGMN1 produces thusin. Both thusin sensitive and resistant strains were detected among clinical isolates of Streptococcus agalactiae. Random mutagenesis of a thusin sensitive strain, S. agalactiae B782, was performed in an attempt to identify the receptor protein for thusin. Three independent thusin resistant mutants were selected and their complete genomes sequenced. Comparative sequence analysis of these mutants with the WT strain revealed that duplication of a region encoding a 79 amino acids repeat in a C-protein α-antigen was a common difference, suggesting it to be responsible for increased resistance to thusin. Since induced thusin resistant mutants showed higher level of resistance than the naturally resistant B761 strain, complete genome sequencing of strain B761 was performed to check the integrity of the C-protein α-antigen-encoding gene. This analysis revealed that this gene is deleted in B761, providing further evidence that this protein promotes interaction of the thusin with receptor.",
publisher = "Springer Nature",
journal = "Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology",
title = "C-protein α-antigen modulates the lantibiotic thusin resistance in Streptococcus agalactiae",
volume = "114",
pages = "1595-1607",
doi = "10.1007/s10482-021-01626-3"
}

Mirković, N., Obradović, M., O’Connor, P. M., Filipić, B., Jovčić, B., Cotter, P. D.,& Kojić, M.. (2021). C-protein α-antigen modulates the lantibiotic thusin resistance in Streptococcus agalactiae. in Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology
Springer Nature., 114, 1595-1607.
https://doi.org/10.1007/s10482-021-01626-3

Mirković N, Obradović M, O’Connor PM, Filipić B, Jovčić B, Cotter PD, Kojić M. C-protein α-antigen modulates the lantibiotic thusin resistance in Streptococcus agalactiae. in Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology. 2021;114:1595-1607.
doi:10.1007/s10482-021-01626-3 .

Mirković, Nemanja, Obradović, Mina, O’Connor, Paula M., Filipić, Brankica, Jovčić, Branko, Cotter, Paul D., Kojić, Milan, "C-protein α-antigen modulates the lantibiotic thusin resistance in Streptococcus agalactiae" in Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology, 114 (2021):1595-1607,
https://doi.org/10.1007/s10482-021-01626-3 . .

TY  - JOUR
AU  - Pirković-Čabarkapa, Andrea
AU  - Živković, Lada
AU  - Zlatković-Švenda, Mirjana
AU  - Borozan, Sunčica
AU  - Topalović, Dijana
AU  - Dekanski, Dragana
AU  - Bruić, Marija
AU  - Bajić, Vladan
AU  - Radak-Perović, Marija
AU  - Spremo-Potparević, Biljana
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3629
AB  - Oxidative stress and inflammation are DNA instability factors for rheumatoid arthritis (RA) patients. The aims of this study were to evaluate cytogenetic alterations in Peripheral Blood Lymphocytes (PBL) in two groups of RA patients: the early and the long-term RA group; and to examine potential of concomitant treatment with Methotrexate (MTX) and Dry olive leaf extract (DOLE) against cytogenetic damage in RA patients after a 3-weeks treatment. A total of 32 RA patients and 10 healthy individuals were included. RA patients were equally divided into four groups: two groups with early phase RA (one treated with MTX alone, the other in combination with DOLE); and two long-term phase RA groups (group with active disease and group with low disease activity)-both treated with MTX and DOLE combination. PBL cultures were screened for chromosome aberrations and micronuclei frequencies. Significantly increased frequencies of micronuclei were shown in active phase RA disease (both early and long-term) but not in the group with low disease activity, as compared to controls. Chromosome aberrations were detected for all 4 RA groups. The highest frequencies of micronuclei and chromosome aberrations were found in the long-term active RA group. After 3 weeks-treatment, there were no significant decrease of the micronuclei frequencies compared to baseline, although they were reduced in all RA groups, except for the group with the long-term active disease. High level of cytogenetic damage in RA patients was concordant with duration and activity of the RA disease. At 3 weeks of therapy, neither the combined treatment (MTX+DOLE), nor MTX alone did not affect the frequency of micronuclei formation.
PB  - Serbian Genetics Society
T2  - Genetika
T1  - Cytogenetic alterations in rheumatoid arthritis patients treated with methotrexate and dry olive leaf extrac
VL  - 52
IS  - 1
SP  - 67
EP  - 80
DO  - 10.2298/GENSR2001067P
ER  - 

@article{
author = "Pirković-Čabarkapa, Andrea and Živković, Lada and Zlatković-Švenda, Mirjana and Borozan, Sunčica and Topalović, Dijana and Dekanski, Dragana and Bruić, Marija and Bajić, Vladan and Radak-Perović, Marija and Spremo-Potparević, Biljana",
year = "2020",
abstract = "Oxidative stress and inflammation are DNA instability factors for rheumatoid arthritis (RA) patients. The aims of this study were to evaluate cytogenetic alterations in Peripheral Blood Lymphocytes (PBL) in two groups of RA patients: the early and the long-term RA group; and to examine potential of concomitant treatment with Methotrexate (MTX) and Dry olive leaf extract (DOLE) against cytogenetic damage in RA patients after a 3-weeks treatment. A total of 32 RA patients and 10 healthy individuals were included. RA patients were equally divided into four groups: two groups with early phase RA (one treated with MTX alone, the other in combination with DOLE); and two long-term phase RA groups (group with active disease and group with low disease activity)-both treated with MTX and DOLE combination. PBL cultures were screened for chromosome aberrations and micronuclei frequencies. Significantly increased frequencies of micronuclei were shown in active phase RA disease (both early and long-term) but not in the group with low disease activity, as compared to controls. Chromosome aberrations were detected for all 4 RA groups. The highest frequencies of micronuclei and chromosome aberrations were found in the long-term active RA group. After 3 weeks-treatment, there were no significant decrease of the micronuclei frequencies compared to baseline, although they were reduced in all RA groups, except for the group with the long-term active disease. High level of cytogenetic damage in RA patients was concordant with duration and activity of the RA disease. At 3 weeks of therapy, neither the combined treatment (MTX+DOLE), nor MTX alone did not affect the frequency of micronuclei formation.",
publisher = "Serbian Genetics Society",
journal = "Genetika",
title = "Cytogenetic alterations in rheumatoid arthritis patients treated with methotrexate and dry olive leaf extrac",
volume = "52",
number = "1",
pages = "67-80",
doi = "10.2298/GENSR2001067P"
}

Pirković-Čabarkapa, A., Živković, L., Zlatković-Švenda, M., Borozan, S., Topalović, D., Dekanski, D., Bruić, M., Bajić, V., Radak-Perović, M.,& Spremo-Potparević, B.. (2020). Cytogenetic alterations in rheumatoid arthritis patients treated with methotrexate and dry olive leaf extrac. in Genetika
Serbian Genetics Society., 52(1), 67-80.
https://doi.org/10.2298/GENSR2001067P

Pirković-Čabarkapa A, Živković L, Zlatković-Švenda M, Borozan S, Topalović D, Dekanski D, Bruić M, Bajić V, Radak-Perović M, Spremo-Potparević B. Cytogenetic alterations in rheumatoid arthritis patients treated with methotrexate and dry olive leaf extrac. in Genetika. 2020;52(1):67-80.
doi:10.2298/GENSR2001067P .

Pirković-Čabarkapa, Andrea, Živković, Lada, Zlatković-Švenda, Mirjana, Borozan, Sunčica, Topalović, Dijana, Dekanski, Dragana, Bruić, Marija, Bajić, Vladan, Radak-Perović, Marija, Spremo-Potparević, Biljana, "Cytogenetic alterations in rheumatoid arthritis patients treated with methotrexate and dry olive leaf extrac" in Genetika, 52, no. 1 (2020):67-80,
https://doi.org/10.2298/GENSR2001067P . .

TY  - JOUR
AU  - Filipić, Brankica
AU  - Novović, Katarina
AU  - Studholme, David
AU  - Malešević, Milka
AU  - Mirković, Nemanja
AU  - Kojić, Milan
AU  - Jovčić, Branko
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3631
AB  - Long-term overuse of antibiotics has driven the propagation and spreading of antibiotic resistance genes (ARGs) such as efflux pumps in the environment, which can be transferred to clinically relevant pathogens. This study explored the abundance and diversity of ARGs and mobile genetic elements within bacterial communities from sediments of three Western Balkans glacial lakes: Plav Lake (high impact of human population), Black Lake (medium impact of human population) and Donje Bare Lake (remote lake, minimal impact of human population) via shotgun metagenomics. Assembled metagenomic sequences revealed that Resistance-Nodulation-Division (RND) efflux pumps genes were most abundant in metagenome from the Plav Lake. The Integron Finder bioinformatics tool detected 38 clusters of attC sites lacking integron-integrases (CALIN) elements: 20 from Plav Lake, four from Black Lake and 14 from Donje Bare Lake. A complete integron sequence was recovered only from the assembled metagenome from Plav Lake. Plasmid contents within the metagenomes were similar, with proportions of contigs being plasmid-related: 1.73% for Plav Lake, 1.59% for Black Lake and 1.64% for Donje Bare Lake. The investigation showed that RNDs and mobile genetic elements content correlated with human population impact.
PB  - NLM (Medline)
T2  - Journal of water and health
T1  - Shotgun metagenomics reveals differences in antibiotic resistance genes among bacterial communities in Western Balkans glacial lakes sediments
VL  - 18
IS  - 3
SP  - 383
EP  - 397
DO  - 10.2166/wh.2020.227
ER  - 

@article{
author = "Filipić, Brankica and Novović, Katarina and Studholme, David and Malešević, Milka and Mirković, Nemanja and Kojić, Milan and Jovčić, Branko",
year = "2020",
abstract = "Long-term overuse of antibiotics has driven the propagation and spreading of antibiotic resistance genes (ARGs) such as efflux pumps in the environment, which can be transferred to clinically relevant pathogens. This study explored the abundance and diversity of ARGs and mobile genetic elements within bacterial communities from sediments of three Western Balkans glacial lakes: Plav Lake (high impact of human population), Black Lake (medium impact of human population) and Donje Bare Lake (remote lake, minimal impact of human population) via shotgun metagenomics. Assembled metagenomic sequences revealed that Resistance-Nodulation-Division (RND) efflux pumps genes were most abundant in metagenome from the Plav Lake. The Integron Finder bioinformatics tool detected 38 clusters of attC sites lacking integron-integrases (CALIN) elements: 20 from Plav Lake, four from Black Lake and 14 from Donje Bare Lake. A complete integron sequence was recovered only from the assembled metagenome from Plav Lake. Plasmid contents within the metagenomes were similar, with proportions of contigs being plasmid-related: 1.73% for Plav Lake, 1.59% for Black Lake and 1.64% for Donje Bare Lake. The investigation showed that RNDs and mobile genetic elements content correlated with human population impact.",
publisher = "NLM (Medline)",
journal = "Journal of water and health",
title = "Shotgun metagenomics reveals differences in antibiotic resistance genes among bacterial communities in Western Balkans glacial lakes sediments",
volume = "18",
number = "3",
pages = "383-397",
doi = "10.2166/wh.2020.227"
}

Filipić, B., Novović, K., Studholme, D., Malešević, M., Mirković, N., Kojić, M.,& Jovčić, B.. (2020). Shotgun metagenomics reveals differences in antibiotic resistance genes among bacterial communities in Western Balkans glacial lakes sediments. in Journal of water and health
NLM (Medline)., 18(3), 383-397.
https://doi.org/10.2166/wh.2020.227

Filipić B, Novović K, Studholme D, Malešević M, Mirković N, Kojić M, Jovčić B. Shotgun metagenomics reveals differences in antibiotic resistance genes among bacterial communities in Western Balkans glacial lakes sediments. in Journal of water and health. 2020;18(3):383-397.
doi:10.2166/wh.2020.227 .

Filipić, Brankica, Novović, Katarina, Studholme, David, Malešević, Milka, Mirković, Nemanja, Kojić, Milan, Jovčić, Branko, "Shotgun metagenomics reveals differences in antibiotic resistance genes among bacterial communities in Western Balkans glacial lakes sediments" in Journal of water and health, 18, no. 3 (2020):383-397,
https://doi.org/10.2166/wh.2020.227 . .

TY  - JOUR
AU  - Miljković, Marija
AU  - Jovanović, Sofija
AU  - O'Connor, Paula M.
AU  - Mirković, Nemanja
AU  - Jovčić, Branko
AU  - Filipić, Brankica
AU  - Dinić, Miroslav
AU  - Studholme, David John
AU  - Fira, Đorđe
AU  - Cotter, Paul D.
AU  - Kojić, Milan
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3372
AB  - Bacteria active against multi-drug resistant pathogens, isolated by direct selection of colonies from clover silage samples, produce zones of inhibition against two Gram-negative (Klebsiella pneumoniae Ni9 and Pseudomonas aeruginosa MMA83) and two Gram-positive (Staphylococcus aureus ATCC25923 and Listeria monocytogenes ATCC19111) pathogens. Isolates BGSP7, BGSP9, BGSP11 and BGSP12 produced the largest zones of inhibition against all four pathogens when grown in LB broth with aeration at 37 degrees C. Isolates BGSP7, BGSP9, BGSP11 and BGSP12 were identified as Brevibacillus laterosporus and pulsed field gel electrophoresis and extracellular protein profiles showed that three different strains (BGSP7, BGSP9 and BGSP11) were isolated. A semi-native SDS-PAGE (sodium dodecyl sulphate-polyacrylamide gel electrophoresis) gel overlay assay showed that BGSP7 and BGSP9 produce small antimicrobial molecules of about 1.5 kDa, while BGSP11 produces antimicrobial molecules of 1.5 and 6 kDa active against S. aureus ATCC25923. Amino acid analysis of two antimicrobial molecules (1583.73 Da; from BGSP7 and 1556.31 Da; from BGSP11) revealed that they have a similar composition and differ only by virtue of the presence of a methionine which is present only in BGSP11 molecule. Genome sequencing of the three isolates revealed the presence of gene clusters associated with the production of non-ribosomally synthesized peptides (brevibacillin, bogorol, gramicidin S, plipastatin and tyrocin) and bacteriocins (laterosporulin, a lactococcin 972-like bacteriocin, as well as putative linocin M18, sactipeptide, UviB and lantipeptide-like molecules). Ultimately, the purification of a number of antimicrobial molecules from each isolate suggests that they can be considered as potent biocontrol strains that produce an arsenal of antimicrobial molecules active against Gram-positive and Gram-negative multi-resistant pathogens, fungi and insects.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - Brevibacillus laterosporus strains BGSP7, BGSP9 and BGSP11 isolated from silage produce broad spectrum multi-antimicrobials
VL  - 14
IS  - 5
DO  - 10.1371/journal.pone.0216773
ER  - 

@article{
author = "Miljković, Marija and Jovanović, Sofija and O'Connor, Paula M. and Mirković, Nemanja and Jovčić, Branko and Filipić, Brankica and Dinić, Miroslav and Studholme, David John and Fira, Đorđe and Cotter, Paul D. and Kojić, Milan",
year = "2019",
abstract = "Bacteria active against multi-drug resistant pathogens, isolated by direct selection of colonies from clover silage samples, produce zones of inhibition against two Gram-negative (Klebsiella pneumoniae Ni9 and Pseudomonas aeruginosa MMA83) and two Gram-positive (Staphylococcus aureus ATCC25923 and Listeria monocytogenes ATCC19111) pathogens. Isolates BGSP7, BGSP9, BGSP11 and BGSP12 produced the largest zones of inhibition against all four pathogens when grown in LB broth with aeration at 37 degrees C. Isolates BGSP7, BGSP9, BGSP11 and BGSP12 were identified as Brevibacillus laterosporus and pulsed field gel electrophoresis and extracellular protein profiles showed that three different strains (BGSP7, BGSP9 and BGSP11) were isolated. A semi-native SDS-PAGE (sodium dodecyl sulphate-polyacrylamide gel electrophoresis) gel overlay assay showed that BGSP7 and BGSP9 produce small antimicrobial molecules of about 1.5 kDa, while BGSP11 produces antimicrobial molecules of 1.5 and 6 kDa active against S. aureus ATCC25923. Amino acid analysis of two antimicrobial molecules (1583.73 Da; from BGSP7 and 1556.31 Da; from BGSP11) revealed that they have a similar composition and differ only by virtue of the presence of a methionine which is present only in BGSP11 molecule. Genome sequencing of the three isolates revealed the presence of gene clusters associated with the production of non-ribosomally synthesized peptides (brevibacillin, bogorol, gramicidin S, plipastatin and tyrocin) and bacteriocins (laterosporulin, a lactococcin 972-like bacteriocin, as well as putative linocin M18, sactipeptide, UviB and lantipeptide-like molecules). Ultimately, the purification of a number of antimicrobial molecules from each isolate suggests that they can be considered as potent biocontrol strains that produce an arsenal of antimicrobial molecules active against Gram-positive and Gram-negative multi-resistant pathogens, fungi and insects.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "Brevibacillus laterosporus strains BGSP7, BGSP9 and BGSP11 isolated from silage produce broad spectrum multi-antimicrobials",
volume = "14",
number = "5",
doi = "10.1371/journal.pone.0216773"
}

Miljković, M., Jovanović, S., O'Connor, P. M., Mirković, N., Jovčić, B., Filipić, B., Dinić, M., Studholme, D. J., Fira, Đ., Cotter, P. D.,& Kojić, M.. (2019). Brevibacillus laterosporus strains BGSP7, BGSP9 and BGSP11 isolated from silage produce broad spectrum multi-antimicrobials. in PLoS One
Public Library Science, San Francisco., 14(5).
https://doi.org/10.1371/journal.pone.0216773

Miljković M, Jovanović S, O'Connor PM, Mirković N, Jovčić B, Filipić B, Dinić M, Studholme DJ, Fira Đ, Cotter PD, Kojić M. Brevibacillus laterosporus strains BGSP7, BGSP9 and BGSP11 isolated from silage produce broad spectrum multi-antimicrobials. in PLoS One. 2019;14(5).
doi:10.1371/journal.pone.0216773 .

Miljković, Marija, Jovanović, Sofija, O'Connor, Paula M., Mirković, Nemanja, Jovčić, Branko, Filipić, Brankica, Dinić, Miroslav, Studholme, David John, Fira, Đorđe, Cotter, Paul D., Kojić, Milan, "Brevibacillus laterosporus strains BGSP7, BGSP9 and BGSP11 isolated from silage produce broad spectrum multi-antimicrobials" in PLoS One, 14, no. 5 (2019),
https://doi.org/10.1371/journal.pone.0216773 . .

TY  - JOUR
AU  - Novović, Katarina
AU  - Malesević, Milka
AU  - Filipić, Brankica
AU  - Mirković, Nemanja
AU  - Miljković, Marija
AU  - Kojić, Milan
AU  - Jovčić, Branko
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3313
AB  - Pseudomonas aeruginosa, which is a clinically important representative of Pseudomonas spp., has been recognized as causative agent of severe nosocomial infections worldwide. An increase in antibiotic resistance of P. aeruginosa clinical strains could be attributed to their capacity to acquire resistance through mobile genetic elements such as mobile integrons that are present in one-half of multidrug-resistant P. aeruginosa strains. Mobile class 1 integrons are recognized as genetic elements involved in the rapid dissemination of multiple genes encoding for antibiotic resistance. The LexA protein is a major repressor of integrase transcription, but differences in transcription regulation among bacterial species have also been noted. In this study, the promoter activity of class 1 integron integrase gene (intI1) and its variant lacking the LexA binding site in Pseudomonas putida WCS358 wild type, rpoS and psrA was analysed. The results show that the activity of the intI1 gene promoter decreased in the rpoS and psrA mutants in the stationary phase of growth compared to the wild type, which indicates the role of RpoS and PsrA proteins in the positive regulation of integrase transcription. Additionally, it was determined that the activity of the lexA gene promoter decreased in rpoS and psrA, and thus, we propose that PsrA indirectly regulates the intI1 gene promoter activity through regulation of lexA gene expression in co-operation with some additional regulators. In this study, intI1 gene expression was shown to be controlled by two major stress response (SOS and RpoS) regulons, which indicates that integrase has evolved to use both systems to sense the cell status.
PB  - Springer, New York
T2  - Current Microbiology
T1  - PsrA Regulator Connects Cell Physiology and Class 1 Integron Integrase Gene Expression Through the Regulation of lexA Gene Expression in Pseudomonas spp.
VL  - 76
IS  - 3
SP  - 320
EP  - 328
DO  - 10.1007/s00284-019-01626-7
ER  - 

@article{
author = "Novović, Katarina and Malesević, Milka and Filipić, Brankica and Mirković, Nemanja and Miljković, Marija and Kojić, Milan and Jovčić, Branko",
year = "2019",
abstract = "Pseudomonas aeruginosa, which is a clinically important representative of Pseudomonas spp., has been recognized as causative agent of severe nosocomial infections worldwide. An increase in antibiotic resistance of P. aeruginosa clinical strains could be attributed to their capacity to acquire resistance through mobile genetic elements such as mobile integrons that are present in one-half of multidrug-resistant P. aeruginosa strains. Mobile class 1 integrons are recognized as genetic elements involved in the rapid dissemination of multiple genes encoding for antibiotic resistance. The LexA protein is a major repressor of integrase transcription, but differences in transcription regulation among bacterial species have also been noted. In this study, the promoter activity of class 1 integron integrase gene (intI1) and its variant lacking the LexA binding site in Pseudomonas putida WCS358 wild type, rpoS and psrA was analysed. The results show that the activity of the intI1 gene promoter decreased in the rpoS and psrA mutants in the stationary phase of growth compared to the wild type, which indicates the role of RpoS and PsrA proteins in the positive regulation of integrase transcription. Additionally, it was determined that the activity of the lexA gene promoter decreased in rpoS and psrA, and thus, we propose that PsrA indirectly regulates the intI1 gene promoter activity through regulation of lexA gene expression in co-operation with some additional regulators. In this study, intI1 gene expression was shown to be controlled by two major stress response (SOS and RpoS) regulons, which indicates that integrase has evolved to use both systems to sense the cell status.",
publisher = "Springer, New York",
journal = "Current Microbiology",
title = "PsrA Regulator Connects Cell Physiology and Class 1 Integron Integrase Gene Expression Through the Regulation of lexA Gene Expression in Pseudomonas spp.",
volume = "76",
number = "3",
pages = "320-328",
doi = "10.1007/s00284-019-01626-7"
}

Novović, K., Malesević, M., Filipić, B., Mirković, N., Miljković, M., Kojić, M.,& Jovčić, B.. (2019). PsrA Regulator Connects Cell Physiology and Class 1 Integron Integrase Gene Expression Through the Regulation of lexA Gene Expression in Pseudomonas spp.. in Current Microbiology
Springer, New York., 76(3), 320-328.
https://doi.org/10.1007/s00284-019-01626-7

Novović K, Malesević M, Filipić B, Mirković N, Miljković M, Kojić M, Jovčić B. PsrA Regulator Connects Cell Physiology and Class 1 Integron Integrase Gene Expression Through the Regulation of lexA Gene Expression in Pseudomonas spp.. in Current Microbiology. 2019;76(3):320-328.
doi:10.1007/s00284-019-01626-7 .

Novović, Katarina, Malesević, Milka, Filipić, Brankica, Mirković, Nemanja, Miljković, Marija, Kojić, Milan, Jovčić, Branko, "PsrA Regulator Connects Cell Physiology and Class 1 Integron Integrase Gene Expression Through the Regulation of lexA Gene Expression in Pseudomonas spp." in Current Microbiology, 76, no. 3 (2019):320-328,
https://doi.org/10.1007/s00284-019-01626-7 . .

TY  - JOUR
AU  - Malešević, Milka
AU  - Di Lorenzo, Flaviana
AU  - Filipić, Brankica
AU  - Stanisavljević, Nemanja
AU  - Novović, Katarina
AU  - Senerovic, Lidija
AU  - Polović, Natalija
AU  - Molinaro, Antonio
AU  - Kojić, Milan
AU  - Jovčić, Branko
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3538
AB  - Pseudomonas aeruginosa is one of the most common opportunistic pathogens that use quorum sensing (QS) system to regulate virulence factors expression and biofilm development. Delftia sp. 11304 was selected among 663 Gram-negative clinical isolates based on its QS inhibitory activity against P. aeruginosa MMA83 clinical isolate. Whole genome sequencing identified this isolate as D. tsuruhatensis and revealed genetic armamentarium of virulence factors and antibiotic resistance determinants. Ethyl acetate extract of D. tsuruhatensis 11304 culture supernatant (QSI extract) prevented biofilm formation of P. aeruginosa MMA83, but was unable to cause biofilm decomposition. QSI extract showed a synergistic effect in combination with meropenem and gentamycin, against P. aeruginosa MMA83. A dose-dependent reduction of the virulence factors: elastase, rhamnolipid and pyocyanin production by P. aeruginosa MMA83 and significant downregulation of lasI, lasR, rhlI, rhlR, pqs and mvfR expression were observed. Matrix-assisted Laser Desorption Ionization (MALDI) mass spectrometry of D. tsuruhatensis 11304 QSI extract revealed the presence of N-acyl homoserine lactones (AHL) with chain lengths of C12 to C18. The main ion peak was identified as N-octadecanoylhomoserine lactone (C18-HSL). Commercial C18-HSL (20 µM) reduced pyocyanin production as well as mRNA level of the lasI gene. A novel AHL species, dihydroxy-N-octadecanoylhomoserine lactone, was also described.
PB  - NatureResearch
T2  - Scientific Reports
T1  - Pseudomonas aeruginosa quorum sensing inhibition by clinical isolate Delftia tsuruhatensis 11304: involvement of N-octadecanoylhomoserine lactones
VL  - 9
IS  - 1
DO  - 10.1038/s41598-019-52955-3
ER  - 

@article{
author = "Malešević, Milka and Di Lorenzo, Flaviana and Filipić, Brankica and Stanisavljević, Nemanja and Novović, Katarina and Senerovic, Lidija and Polović, Natalija and Molinaro, Antonio and Kojić, Milan and Jovčić, Branko",
year = "2019",
abstract = "Pseudomonas aeruginosa is one of the most common opportunistic pathogens that use quorum sensing (QS) system to regulate virulence factors expression and biofilm development. Delftia sp. 11304 was selected among 663 Gram-negative clinical isolates based on its QS inhibitory activity against P. aeruginosa MMA83 clinical isolate. Whole genome sequencing identified this isolate as D. tsuruhatensis and revealed genetic armamentarium of virulence factors and antibiotic resistance determinants. Ethyl acetate extract of D. tsuruhatensis 11304 culture supernatant (QSI extract) prevented biofilm formation of P. aeruginosa MMA83, but was unable to cause biofilm decomposition. QSI extract showed a synergistic effect in combination with meropenem and gentamycin, against P. aeruginosa MMA83. A dose-dependent reduction of the virulence factors: elastase, rhamnolipid and pyocyanin production by P. aeruginosa MMA83 and significant downregulation of lasI, lasR, rhlI, rhlR, pqs and mvfR expression were observed. Matrix-assisted Laser Desorption Ionization (MALDI) mass spectrometry of D. tsuruhatensis 11304 QSI extract revealed the presence of N-acyl homoserine lactones (AHL) with chain lengths of C12 to C18. The main ion peak was identified as N-octadecanoylhomoserine lactone (C18-HSL). Commercial C18-HSL (20 µM) reduced pyocyanin production as well as mRNA level of the lasI gene. A novel AHL species, dihydroxy-N-octadecanoylhomoserine lactone, was also described.",
publisher = "NatureResearch",
journal = "Scientific Reports",
title = "Pseudomonas aeruginosa quorum sensing inhibition by clinical isolate Delftia tsuruhatensis 11304: involvement of N-octadecanoylhomoserine lactones",
volume = "9",
number = "1",
doi = "10.1038/s41598-019-52955-3"
}

Malešević, M., Di Lorenzo, F., Filipić, B., Stanisavljević, N., Novović, K., Senerovic, L., Polović, N., Molinaro, A., Kojić, M.,& Jovčić, B.. (2019). Pseudomonas aeruginosa quorum sensing inhibition by clinical isolate Delftia tsuruhatensis 11304: involvement of N-octadecanoylhomoserine lactones. in Scientific Reports
NatureResearch., 9(1).
https://doi.org/10.1038/s41598-019-52955-3

Malešević M, Di Lorenzo F, Filipić B, Stanisavljević N, Novović K, Senerovic L, Polović N, Molinaro A, Kojić M, Jovčić B. Pseudomonas aeruginosa quorum sensing inhibition by clinical isolate Delftia tsuruhatensis 11304: involvement of N-octadecanoylhomoserine lactones. in Scientific Reports. 2019;9(1).
doi:10.1038/s41598-019-52955-3 .

Malešević, Milka, Di Lorenzo, Flaviana, Filipić, Brankica, Stanisavljević, Nemanja, Novović, Katarina, Senerovic, Lidija, Polović, Natalija, Molinaro, Antonio, Kojić, Milan, Jovčić, Branko, "Pseudomonas aeruginosa quorum sensing inhibition by clinical isolate Delftia tsuruhatensis 11304: involvement of N-octadecanoylhomoserine lactones" in Scientific Reports, 9, no. 1 (2019),
https://doi.org/10.1038/s41598-019-52955-3 . .

TY  - JOUR
AU  - Malesević, Milka
AU  - Mirković, Nemanja
AU  - Lozo, Jelena
AU  - Novović, Katarina
AU  - Filipić, Brankica
AU  - Kojić, Milan
AU  - Jovčić, Branko
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3333
AB  - 16S rRNA gene-based metagenomic approach was used to assess the biodiversity of bacterial communities in the sediments of selected glacial lakes in the Western Balkans and to assess the impact of human population on these microbial communities. Sediment samples were collected from three glacial lakes, viz., Plav Lake (in a zone of the highest impact of human population), Black Lake (a zone of medium impact of human population), and Donje Bare Lake (a remote lake with minimal impact of human population). Canonical correlation analysis analysis indicated correlation between the distance of the lake from urbanized population and bacterial diversity in Donje Bare Lake sediment. Bacterial diversity of Black Lake sediment was correlated with high content of phosphorous and pH value. Chemical compounds exhibiting the most prominent correlation with bacterial diversity of Plav Lake were NH4-N, K2O, CaCo3, and total nitrogen . Additionally, CCA analysis indicated that population density was correlated with biodiversity of bacterial communities in Plav Lake sediment, which is the most exposed to human population. Multivariate regression revealed the highest correlation between the presence of Proteobacteria classes and population density and levels of NH4-N. The influence of human population was observed to be important for shaping the sediment communities in addition to biological and chemical factors.
PB  - Taylor & Francis Inc, Philadelphia
T2  - Geomicrobiology Journal
T1  - Bacterial Diversity among the Sediments of Glacial Lakes in the Western Balkans: Exploring the Impact of Human Population
VL  - 36
IS  - 3
SP  - 261
EP  - 270
DO  - 10.1080/01490451.2018.1550128
ER  - 

@article{
author = "Malesević, Milka and Mirković, Nemanja and Lozo, Jelena and Novović, Katarina and Filipić, Brankica and Kojić, Milan and Jovčić, Branko",
year = "2019",
abstract = "16S rRNA gene-based metagenomic approach was used to assess the biodiversity of bacterial communities in the sediments of selected glacial lakes in the Western Balkans and to assess the impact of human population on these microbial communities. Sediment samples were collected from three glacial lakes, viz., Plav Lake (in a zone of the highest impact of human population), Black Lake (a zone of medium impact of human population), and Donje Bare Lake (a remote lake with minimal impact of human population). Canonical correlation analysis analysis indicated correlation between the distance of the lake from urbanized population and bacterial diversity in Donje Bare Lake sediment. Bacterial diversity of Black Lake sediment was correlated with high content of phosphorous and pH value. Chemical compounds exhibiting the most prominent correlation with bacterial diversity of Plav Lake were NH4-N, K2O, CaCo3, and total nitrogen . Additionally, CCA analysis indicated that population density was correlated with biodiversity of bacterial communities in Plav Lake sediment, which is the most exposed to human population. Multivariate regression revealed the highest correlation between the presence of Proteobacteria classes and population density and levels of NH4-N. The influence of human population was observed to be important for shaping the sediment communities in addition to biological and chemical factors.",
publisher = "Taylor & Francis Inc, Philadelphia",
journal = "Geomicrobiology Journal",
title = "Bacterial Diversity among the Sediments of Glacial Lakes in the Western Balkans: Exploring the Impact of Human Population",
volume = "36",
number = "3",
pages = "261-270",
doi = "10.1080/01490451.2018.1550128"
}

Malesević, M., Mirković, N., Lozo, J., Novović, K., Filipić, B., Kojić, M.,& Jovčić, B.. (2019). Bacterial Diversity among the Sediments of Glacial Lakes in the Western Balkans: Exploring the Impact of Human Population. in Geomicrobiology Journal
Taylor & Francis Inc, Philadelphia., 36(3), 261-270.
https://doi.org/10.1080/01490451.2018.1550128

Malesević M, Mirković N, Lozo J, Novović K, Filipić B, Kojić M, Jovčić B. Bacterial Diversity among the Sediments of Glacial Lakes in the Western Balkans: Exploring the Impact of Human Population. in Geomicrobiology Journal. 2019;36(3):261-270.
doi:10.1080/01490451.2018.1550128 .

Malesević, Milka, Mirković, Nemanja, Lozo, Jelena, Novović, Katarina, Filipić, Brankica, Kojić, Milan, Jovčić, Branko, "Bacterial Diversity among the Sediments of Glacial Lakes in the Western Balkans: Exploring the Impact of Human Population" in Geomicrobiology Journal, 36, no. 3 (2019):261-270,
https://doi.org/10.1080/01490451.2018.1550128 . .

TY  - JOUR
AU  - Lilić, Branislav
AU  - Filipić, Brankica
AU  - Malesević, Milka
AU  - Novović, Katarina
AU  - Vasiljević, Zorica
AU  - Kojić, Milan
AU  - Jovčić, Branko
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3298
AB  - The aim of this study was to evaluate the contribution of plasmid-mediated genes and efflux to fluoroquinolone resistance in collection of Achromobacter spp. gathered during a 3-year period. Susceptibility to ciprofloxacin and levofloxacin was tested by disk diffusion and microdilution tests for a collection of 98 Achromobacter spp. clinical isolates. Identification of fluoroquinolone-resistant isolates was performed by sequencing and phylogenetic analyses of the nrdA gene. Genetic relatedness among resistant isolates was determined by pulsed-field gel electrophoresis (PFGE) analysis. The influence of an H+ conductor cyanide m-chlorophenyl hydrazone (CCCP) and a resistance-nodulation-division-type efflux pump inhibitor phenylalanine-arginine beta-naphthylamide (PAN) on minimal inhibitory concentration (MIC) value was evaluated by broth microdilution. The presence of the plasmid-mediated qnrA, qnrB, qnrC, qnrS, and aac-(6)-Ib-cr genes was investigated by PCR and sequencing. Achromobacter spp. isolates that were resistant or intermediately resistant to fluoroquinolones in disk diffusion tests (44/98) were subjected to microdilution. As a result, 20/98 isolates were confirmed to be resistant to ciprofloxacin while 10/98 was resistant to levofloxacin. CCCP decreased twofold MIC value for ciprofloxacin in six isolates and more than 16 times in one isolate, while MIC value for levofloxacin was decreased in all isolates (twofold to more than eightfold). Fluoroquinolone-resistant isolates were identified as A. xylosoxidans with the nrdA gene sequencing. PFGE revealed that resistant isolates belonged to seven different genotypes. Ten isolates belonging to four genotypes were positive for the aac-(6)-Ib-cr gene. Although resistance to fluoroquinolones was not widespread among analyzed isolates, detected contribution of efflux pumps and the presence of the aac-(6)-Ib-cr gene present a platform for emergence of more resistant strains.
PB  - Springer, Dordrecht
T2  - Folia Microbiologica
T1  - Fluoroquinolone-resistant Achromobacter xylosoxidans clinical isolates from Serbia: high prevalence of the aac-(6)-Ib-cr gene among resistant isolates
VL  - 64
IS  - 2
SP  - 153
EP  - 159
DO  - 10.1007/s12223-018-0639-7
ER  - 

@article{
author = "Lilić, Branislav and Filipić, Brankica and Malesević, Milka and Novović, Katarina and Vasiljević, Zorica and Kojić, Milan and Jovčić, Branko",
year = "2019",
abstract = "The aim of this study was to evaluate the contribution of plasmid-mediated genes and efflux to fluoroquinolone resistance in collection of Achromobacter spp. gathered during a 3-year period. Susceptibility to ciprofloxacin and levofloxacin was tested by disk diffusion and microdilution tests for a collection of 98 Achromobacter spp. clinical isolates. Identification of fluoroquinolone-resistant isolates was performed by sequencing and phylogenetic analyses of the nrdA gene. Genetic relatedness among resistant isolates was determined by pulsed-field gel electrophoresis (PFGE) analysis. The influence of an H+ conductor cyanide m-chlorophenyl hydrazone (CCCP) and a resistance-nodulation-division-type efflux pump inhibitor phenylalanine-arginine beta-naphthylamide (PAN) on minimal inhibitory concentration (MIC) value was evaluated by broth microdilution. The presence of the plasmid-mediated qnrA, qnrB, qnrC, qnrS, and aac-(6)-Ib-cr genes was investigated by PCR and sequencing. Achromobacter spp. isolates that were resistant or intermediately resistant to fluoroquinolones in disk diffusion tests (44/98) were subjected to microdilution. As a result, 20/98 isolates were confirmed to be resistant to ciprofloxacin while 10/98 was resistant to levofloxacin. CCCP decreased twofold MIC value for ciprofloxacin in six isolates and more than 16 times in one isolate, while MIC value for levofloxacin was decreased in all isolates (twofold to more than eightfold). Fluoroquinolone-resistant isolates were identified as A. xylosoxidans with the nrdA gene sequencing. PFGE revealed that resistant isolates belonged to seven different genotypes. Ten isolates belonging to four genotypes were positive for the aac-(6)-Ib-cr gene. Although resistance to fluoroquinolones was not widespread among analyzed isolates, detected contribution of efflux pumps and the presence of the aac-(6)-Ib-cr gene present a platform for emergence of more resistant strains.",
publisher = "Springer, Dordrecht",
journal = "Folia Microbiologica",
title = "Fluoroquinolone-resistant Achromobacter xylosoxidans clinical isolates from Serbia: high prevalence of the aac-(6)-Ib-cr gene among resistant isolates",
volume = "64",
number = "2",
pages = "153-159",
doi = "10.1007/s12223-018-0639-7"
}

Lilić, B., Filipić, B., Malesević, M., Novović, K., Vasiljević, Z., Kojić, M.,& Jovčić, B.. (2019). Fluoroquinolone-resistant Achromobacter xylosoxidans clinical isolates from Serbia: high prevalence of the aac-(6)-Ib-cr gene among resistant isolates. in Folia Microbiologica
Springer, Dordrecht., 64(2), 153-159.
https://doi.org/10.1007/s12223-018-0639-7

Lilić B, Filipić B, Malesević M, Novović K, Vasiljević Z, Kojić M, Jovčić B. Fluoroquinolone-resistant Achromobacter xylosoxidans clinical isolates from Serbia: high prevalence of the aac-(6)-Ib-cr gene among resistant isolates. in Folia Microbiologica. 2019;64(2):153-159.
doi:10.1007/s12223-018-0639-7 .

Lilić, Branislav, Filipić, Brankica, Malesević, Milka, Novović, Katarina, Vasiljević, Zorica, Kojić, Milan, Jovčić, Branko, "Fluoroquinolone-resistant Achromobacter xylosoxidans clinical isolates from Serbia: high prevalence of the aac-(6)-Ib-cr gene among resistant isolates" in Folia Microbiologica, 64, no. 2 (2019):153-159,
https://doi.org/10.1007/s12223-018-0639-7 . .

TY  - JOUR
AU  - Dinić, Miroslav
AU  - Pecikoza, Uroš
AU  - Đokić, Jelena
AU  - Stepanović-Petrović, Radica
AU  - Milenković, Marina
AU  - Stevanović, Magdalena
AU  - Filipović, Nenad
AU  - Begović, Jelena
AU  - Golić, Nataša
AU  - Lukić, Jovanka
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3152
AB  - The aim of this study was to test the potential of high molecular weight exopolysaccharide (EPS) produced by the putative probiotic strain Lactobacillus paraplantarum BGCG11 (EPS CG11) to alleviate inflammatory pain in Wistar rats. The EPS CG11 was isolated from bacterial surface and was subjected to Fourier-transform infrared spectroscopy (FTIR) and thermal analysis. FTIR spectra confirmed the polysaccharide structure of isolated sample, while the thermal methods revealed good thermal properties of the polymer. The antihyperalgesic and antiedematous effects of the EPS CG11 were examined in the rat model of inflammation induced by carrageenan injection in hind paw. The results showed that the intraperitoneal administration of EPS CG11 produced a significant decrease in pain sensations (mechanical hyperalgesia) and a paw swelling in a dose-dependent manner as it was measured using Von Frey anesthesiometer and plethysmometer, respectively. These effects were followed by a decreased expression of IL-1 beta and iNOS mRNAs in rat's paw tissue suggesting that the antihyperalgesic and antiedematous effects of the EPS CG11 are related to the suppression of inflammatory response. Additionally, we demonstrated that EPS CG11 exhibits immunosuppressive properties in the peritonitis model induced by carrageenan. Expression levels of pro-inflammatory mediators IL-1 beta, TNF-alpha and iNOS were decreased, together with the enhanced secretion of anti-inflammatory IL-10 and IL-6 cytokines, while neutrophil infiltration was not changed. To the best of our knowledge, this is the first study which reports an antihyperalgesic effect as the novel property of bacterial EPSs. Given the high demands of pharmaceutical industry for the replacement of commonly used analgesics due to numerous side effects, this study describes a promising natural compound for the future pharmacological testing in the area.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Pharmacology
T1  - Exopolysaccharide Produced by Probiotic Strain Lactobacillus paraplantarum BGCG11 Reduces Inflammatory Hyperalgesia in Rats
VL  - 9
DO  - 10.3389/fphar.2018.00001
ER  - 

@article{
author = "Dinić, Miroslav and Pecikoza, Uroš and Đokić, Jelena and Stepanović-Petrović, Radica and Milenković, Marina and Stevanović, Magdalena and Filipović, Nenad and Begović, Jelena and Golić, Nataša and Lukić, Jovanka",
year = "2018",
abstract = "The aim of this study was to test the potential of high molecular weight exopolysaccharide (EPS) produced by the putative probiotic strain Lactobacillus paraplantarum BGCG11 (EPS CG11) to alleviate inflammatory pain in Wistar rats. The EPS CG11 was isolated from bacterial surface and was subjected to Fourier-transform infrared spectroscopy (FTIR) and thermal analysis. FTIR spectra confirmed the polysaccharide structure of isolated sample, while the thermal methods revealed good thermal properties of the polymer. The antihyperalgesic and antiedematous effects of the EPS CG11 were examined in the rat model of inflammation induced by carrageenan injection in hind paw. The results showed that the intraperitoneal administration of EPS CG11 produced a significant decrease in pain sensations (mechanical hyperalgesia) and a paw swelling in a dose-dependent manner as it was measured using Von Frey anesthesiometer and plethysmometer, respectively. These effects were followed by a decreased expression of IL-1 beta and iNOS mRNAs in rat's paw tissue suggesting that the antihyperalgesic and antiedematous effects of the EPS CG11 are related to the suppression of inflammatory response. Additionally, we demonstrated that EPS CG11 exhibits immunosuppressive properties in the peritonitis model induced by carrageenan. Expression levels of pro-inflammatory mediators IL-1 beta, TNF-alpha and iNOS were decreased, together with the enhanced secretion of anti-inflammatory IL-10 and IL-6 cytokines, while neutrophil infiltration was not changed. To the best of our knowledge, this is the first study which reports an antihyperalgesic effect as the novel property of bacterial EPSs. Given the high demands of pharmaceutical industry for the replacement of commonly used analgesics due to numerous side effects, this study describes a promising natural compound for the future pharmacological testing in the area.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Pharmacology",
title = "Exopolysaccharide Produced by Probiotic Strain Lactobacillus paraplantarum BGCG11 Reduces Inflammatory Hyperalgesia in Rats",
volume = "9",
doi = "10.3389/fphar.2018.00001"
}

Dinić, M., Pecikoza, U., Đokić, J., Stepanović-Petrović, R., Milenković, M., Stevanović, M., Filipović, N., Begović, J., Golić, N.,& Lukić, J.. (2018). Exopolysaccharide Produced by Probiotic Strain Lactobacillus paraplantarum BGCG11 Reduces Inflammatory Hyperalgesia in Rats. in Frontiers in Pharmacology
Frontiers Media Sa, Lausanne., 9.
https://doi.org/10.3389/fphar.2018.00001

Dinić M, Pecikoza U, Đokić J, Stepanović-Petrović R, Milenković M, Stevanović M, Filipović N, Begović J, Golić N, Lukić J. Exopolysaccharide Produced by Probiotic Strain Lactobacillus paraplantarum BGCG11 Reduces Inflammatory Hyperalgesia in Rats. in Frontiers in Pharmacology. 2018;9.
doi:10.3389/fphar.2018.00001 .

Dinić, Miroslav, Pecikoza, Uroš, Đokić, Jelena, Stepanović-Petrović, Radica, Milenković, Marina, Stevanović, Magdalena, Filipović, Nenad, Begović, Jelena, Golić, Nataša, Lukić, Jovanka, "Exopolysaccharide Produced by Probiotic Strain Lactobacillus paraplantarum BGCG11 Reduces Inflammatory Hyperalgesia in Rats" in Frontiers in Pharmacology, 9 (2018),
https://doi.org/10.3389/fphar.2018.00001 . .

TY  - THES
AU  - Dinić, Miroslav
PY  - 2018
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=6030
UR  - http://nardus.mpn.gov.rs/handle/123456789/9986
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:18343/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=2048264546
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3680
AB  - Izolacija i karakterizacija bakterijskih biomolekula koji interaguju sa receptorimaćelija domaćina predstavlja kljuĉ za razumevanje mehanizama probiotiĉkog dejstvalaktobacila. Savremena istraţivanja probiotiĉkih bakterija usmerena su ka identifikacijibiomolekula koji mogu modulisati razliĉite signalne puteve u humanim ćelijama. Svimolekuli koji su poreklom iz probiotika i odgovorni su za njihov pozitivan efekat nazdravlje domaćina nazvani su postbiotici. Zbog slabijeg imunostimulišućeg potencijala,njihova primena predstavlja bezbednu alternativu primeni ţivih bakterija.Ovo istraţivanje je imalo za cilj da testira mogućnost primene postbiotika uublaţavanju simptoma bola i neţeljenih efekata koji nastaju kao posledica primeneanalgetika. Taĉnije, definisana su dva specifiĉna cilja istraţivanja: (i) ispitati uticajbioaktivnih molekula odabranih sojeva laktobacila na proces autofagije u hepatocitima invitro, kao i protektivan efekat ovih molekula kod toksiĉnosti izazvane paracetamolom i (ii)ispitati potencijalni imunomodulatorni efekat egzopolisaharida (EPS-CG11) izolovanog izsoja Lactobacillus paraplantarum BGCG11, u in vivo eksperimentalnim modelimainflamacije. Eksperimenti u kulturi hepatocita ukljuĉili su praćenje stepena oštećenjaHepG2 ćelija izloţenih toksiĉnoj koncentraciji paracetamola i procesa autofagije, sa ciljemidentifikacije potencijalnog mehanizma delovanja postbiotika. Metodološki, vijabilnostHepG2 ćelija analizirana je MTT i LDH esejima. Autofagija je praćena Western blotanalizom odreĊivanjem ekspresije p62/SQSTM1 proteina i akumulacijom liposolubilneforme LC3 proteina. Dodatno, autofagija je analizirana praćenjem ekspresije BECN1, Atg5,p62/SQSTM1 i PINK1 gena i autofagnog fluksa. Za analizu imunomodulatornog efektaEPS-CG11 korišćena su dva in vivo modela inflamacije izazvane karageninom: modelinflamatornog bola i model peritonitisa kod pacova Wistar soja. U eksperimentuinflamatornog bola praćeni su: vremenski tok razvoja hiperalgezije i edema šapica nakonprofilaktiĉke sistemske primene EPS-CG11, ekspresija medijatora inflamacije (IL-1β,TNF-α, IL-6 i iNOS), infiltracija neutrofila (ekspresija MPO enzima) i aktivacija/infiltracijamonocita (ekspresija CD14 markera)...
AB  - Isolation and characterization of bacterial biomolecules involved in the interactionwith the receptors of the host cells represent the key factor for understanding themechanisms of probiotic action of lactobacilli. Novel studies regarding probiotic bacteriahave been focused on the identification of biomolecules which can modulate differentsignaling pathways in human cells. All molecules that originate from probiotics which areresponsible for its positive effects on the host’s health are called postbiotics. Theirapplication represents the safe alternative to the use of live bacteria and itsimmunostimulating potential.This research aimed to test the possibility of using postbiotics in alleviation of painsymptoms and analgesics side effects. More precisely, two main objectives of this researchwere: (i) to examine the influence of bioactive molecules of selected strains of lactobacillion the autophagy process in the hepatocytes, in vitro, as well as protective effect of thesemolecules in paracetamol-induced toxicity and (ii) to examine the potentialimmunomodulatory effect of exopolysaccharide (EPS-CG11) isolated from Lactobacillusparaplantarum BGCG11 strain, in in vivo experimental models of inflammation.Experiments in the hepatocytes culture included monitoring the degree of damage ofHepG2 cells exposed to the toxic paracetamol concentration and the autophagy process,with the aim of identification of potential mechanism of postbiotic action.Methodologically, the cell viability was monitored by MTT and LDH assays. Autophagywas monitored by Western blot analysis, in order to determine the expression of thep62/SQSTM1 protein and the accumulation of the liposoluble form of the LC3 protein.Further, the autophagy was analyzed by monitoring the expression of BECN1, Atg5,p62/SQSTM1 and PINK1 genes and the autophagy flux. For the analysis of theimmunomodulatory effect of EPS-CG11, two in vivo models of carrageenan-inducedinflammation were used: an inflammatory pain model and a peritonitis model in the Wistarrats...
PB  - Универзитет у Београду, Фармацеутски факултет
T2  - Универзитет у Београду
T1  - Uticaj bioaktivnih molekula laktobacila na procese autofagije i inflamacije u in vitro i in vivo sistemima
UR  - https://hdl.handle.net/21.15107/rcub_nardus_9986
ER  - 

@phdthesis{
author = "Dinić, Miroslav",
year = "2018",
abstract = "Izolacija i karakterizacija bakterijskih biomolekula koji interaguju sa receptorimaćelija domaćina predstavlja kljuĉ za razumevanje mehanizama probiotiĉkog dejstvalaktobacila. Savremena istraţivanja probiotiĉkih bakterija usmerena su ka identifikacijibiomolekula koji mogu modulisati razliĉite signalne puteve u humanim ćelijama. Svimolekuli koji su poreklom iz probiotika i odgovorni su za njihov pozitivan efekat nazdravlje domaćina nazvani su postbiotici. Zbog slabijeg imunostimulišućeg potencijala,njihova primena predstavlja bezbednu alternativu primeni ţivih bakterija.Ovo istraţivanje je imalo za cilj da testira mogućnost primene postbiotika uublaţavanju simptoma bola i neţeljenih efekata koji nastaju kao posledica primeneanalgetika. Taĉnije, definisana su dva specifiĉna cilja istraţivanja: (i) ispitati uticajbioaktivnih molekula odabranih sojeva laktobacila na proces autofagije u hepatocitima invitro, kao i protektivan efekat ovih molekula kod toksiĉnosti izazvane paracetamolom i (ii)ispitati potencijalni imunomodulatorni efekat egzopolisaharida (EPS-CG11) izolovanog izsoja Lactobacillus paraplantarum BGCG11, u in vivo eksperimentalnim modelimainflamacije. Eksperimenti u kulturi hepatocita ukljuĉili su praćenje stepena oštećenjaHepG2 ćelija izloţenih toksiĉnoj koncentraciji paracetamola i procesa autofagije, sa ciljemidentifikacije potencijalnog mehanizma delovanja postbiotika. Metodološki, vijabilnostHepG2 ćelija analizirana je MTT i LDH esejima. Autofagija je praćena Western blotanalizom odreĊivanjem ekspresije p62/SQSTM1 proteina i akumulacijom liposolubilneforme LC3 proteina. Dodatno, autofagija je analizirana praćenjem ekspresije BECN1, Atg5,p62/SQSTM1 i PINK1 gena i autofagnog fluksa. Za analizu imunomodulatornog efektaEPS-CG11 korišćena su dva in vivo modela inflamacije izazvane karageninom: modelinflamatornog bola i model peritonitisa kod pacova Wistar soja. U eksperimentuinflamatornog bola praćeni su: vremenski tok razvoja hiperalgezije i edema šapica nakonprofilaktiĉke sistemske primene EPS-CG11, ekspresija medijatora inflamacije (IL-1β,TNF-α, IL-6 i iNOS), infiltracija neutrofila (ekspresija MPO enzima) i aktivacija/infiltracijamonocita (ekspresija CD14 markera)..., Isolation and characterization of bacterial biomolecules involved in the interactionwith the receptors of the host cells represent the key factor for understanding themechanisms of probiotic action of lactobacilli. Novel studies regarding probiotic bacteriahave been focused on the identification of biomolecules which can modulate differentsignaling pathways in human cells. All molecules that originate from probiotics which areresponsible for its positive effects on the host’s health are called postbiotics. Theirapplication represents the safe alternative to the use of live bacteria and itsimmunostimulating potential.This research aimed to test the possibility of using postbiotics in alleviation of painsymptoms and analgesics side effects. More precisely, two main objectives of this researchwere: (i) to examine the influence of bioactive molecules of selected strains of lactobacillion the autophagy process in the hepatocytes, in vitro, as well as protective effect of thesemolecules in paracetamol-induced toxicity and (ii) to examine the potentialimmunomodulatory effect of exopolysaccharide (EPS-CG11) isolated from Lactobacillusparaplantarum BGCG11 strain, in in vivo experimental models of inflammation.Experiments in the hepatocytes culture included monitoring the degree of damage ofHepG2 cells exposed to the toxic paracetamol concentration and the autophagy process,with the aim of identification of potential mechanism of postbiotic action.Methodologically, the cell viability was monitored by MTT and LDH assays. Autophagywas monitored by Western blot analysis, in order to determine the expression of thep62/SQSTM1 protein and the accumulation of the liposoluble form of the LC3 protein.Further, the autophagy was analyzed by monitoring the expression of BECN1, Atg5,p62/SQSTM1 and PINK1 genes and the autophagy flux. For the analysis of theimmunomodulatory effect of EPS-CG11, two in vivo models of carrageenan-inducedinflammation were used: an inflammatory pain model and a peritonitis model in the Wistarrats...",
publisher = "Универзитет у Београду, Фармацеутски факултет",
journal = "Универзитет у Београду",
title = "Uticaj bioaktivnih molekula laktobacila na procese autofagije i inflamacije u in vitro i in vivo sistemima",
url = "https://hdl.handle.net/21.15107/rcub_nardus_9986"
}

Dinić, M.. (2018). Uticaj bioaktivnih molekula laktobacila na procese autofagije i inflamacije u in vitro i in vivo sistemima. in Универзитет у Београду
Универзитет у Београду, Фармацеутски факултет..
https://hdl.handle.net/21.15107/rcub_nardus_9986

Dinić M. Uticaj bioaktivnih molekula laktobacila na procese autofagije i inflamacije u in vitro i in vivo sistemima. in Универзитет у Београду. 2018;.
https://hdl.handle.net/21.15107/rcub_nardus_9986 .

Dinić, Miroslav, "Uticaj bioaktivnih molekula laktobacila na procese autofagije i inflamacije u in vitro i in vivo sistemima" in Универзитет у Београду (2018),
https://hdl.handle.net/21.15107/rcub_nardus_9986 .

TY  - JOUR
AU  - Miljković, Marija
AU  - Malesević, Milka
AU  - Filipić, Brankica
AU  - Vukotić, Goran
AU  - Kojić, Milan
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3192
AB  - Restriction enzymes are the main defence system against foreign DNA, in charge of preserving genome integrity. Lactococcus raffinolactis BGTRK10-1 expresses LraI Type II restriction-modification enzyme, whose activity is similar to that shown for EcoRI; LraI methyltransferase protects DNA from EcoRI cleavage. The gene encoding LraI endonuclease was cloned and overexpressed in E. coli. Purified enzyme showed the highest specific activity at lower temperatures (between 13 degrees C and 37 degrees C) and was stable after storage at -20 degrees C in 50% glycerol. The concentration of monovalent ions in the reaction buffer required for optimal activity of LraI restriction enzyme was 100 mM or higher. The recognition and cleavage sequence for LraI restriction enzyme was determined as 5'-G/AATTC-3', indicating that LraI restriction enzyme is an isoschizomer of EcoRI. In the reaction buffer with a lower salt concentration, LraI exhibits star activity and specifically recognizes and cuts another alternative sequence 5'-A/AATTC-3', leaving the same sticky ends on fragments as EcoRI, which makes them clonable into a linearized vector. Phylogenetic analysis based on sequence alignment pointed out the common origin of LraI restriction-modification system with previously described EcoRI-like restriction-modification systems.
PB  - Hindawi Ltd, London
T2  - Biomed Research International
T1  - LraI from Lactococcus raffinolactis BGTRK10-1, an Isoschizomer of EcoRI, Exhibits Ion Concentration-Dependent Specific Star Activity
DO  - 10.1155/2018/5657085
ER  - 

@article{
author = "Miljković, Marija and Malesević, Milka and Filipić, Brankica and Vukotić, Goran and Kojić, Milan",
year = "2018",
abstract = "Restriction enzymes are the main defence system against foreign DNA, in charge of preserving genome integrity. Lactococcus raffinolactis BGTRK10-1 expresses LraI Type II restriction-modification enzyme, whose activity is similar to that shown for EcoRI; LraI methyltransferase protects DNA from EcoRI cleavage. The gene encoding LraI endonuclease was cloned and overexpressed in E. coli. Purified enzyme showed the highest specific activity at lower temperatures (between 13 degrees C and 37 degrees C) and was stable after storage at -20 degrees C in 50% glycerol. The concentration of monovalent ions in the reaction buffer required for optimal activity of LraI restriction enzyme was 100 mM or higher. The recognition and cleavage sequence for LraI restriction enzyme was determined as 5'-G/AATTC-3', indicating that LraI restriction enzyme is an isoschizomer of EcoRI. In the reaction buffer with a lower salt concentration, LraI exhibits star activity and specifically recognizes and cuts another alternative sequence 5'-A/AATTC-3', leaving the same sticky ends on fragments as EcoRI, which makes them clonable into a linearized vector. Phylogenetic analysis based on sequence alignment pointed out the common origin of LraI restriction-modification system with previously described EcoRI-like restriction-modification systems.",
publisher = "Hindawi Ltd, London",
journal = "Biomed Research International",
title = "LraI from Lactococcus raffinolactis BGTRK10-1, an Isoschizomer of EcoRI, Exhibits Ion Concentration-Dependent Specific Star Activity",
doi = "10.1155/2018/5657085"
}

Miljković, M., Malesević, M., Filipić, B., Vukotić, G.,& Kojić, M.. (2018). LraI from Lactococcus raffinolactis BGTRK10-1, an Isoschizomer of EcoRI, Exhibits Ion Concentration-Dependent Specific Star Activity. in Biomed Research International
Hindawi Ltd, London..
https://doi.org/10.1155/2018/5657085

Miljković M, Malesević M, Filipić B, Vukotić G, Kojić M. LraI from Lactococcus raffinolactis BGTRK10-1, an Isoschizomer of EcoRI, Exhibits Ion Concentration-Dependent Specific Star Activity. in Biomed Research International. 2018;.
doi:10.1155/2018/5657085 .

Miljković, Marija, Malesević, Milka, Filipić, Brankica, Vukotić, Goran, Kojić, Milan, "LraI from Lactococcus raffinolactis BGTRK10-1, an Isoschizomer of EcoRI, Exhibits Ion Concentration-Dependent Specific Star Activity" in Biomed Research International (2018),
https://doi.org/10.1155/2018/5657085 . .

TY  - JOUR
AU  - Dinić, Miroslav
AU  - Lukić, Jovanka
AU  - Đokić, Jelena
AU  - Milenković, Marina
AU  - Strahinić, Ivana
AU  - Golić, Nataša
AU  - Begović, Jelena
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2917
AB  - The aim of this study was to investigate the potential of postbiotics originated from Lactobacillus fermentum BGHV110 strain (HV110) to counteract acetaminophen (APAP)-induced hepatotoxicity in HepG2 cells. This strain was selected according to its autophagy inducing potential, based on previous studies reporting protective role of autophagy in APAP caused cellular damage. Cell viability was assessed using MTT and LDH assays, while autophagy was monitored by qPCR analysis of BECN1, Atg5, p62/SQSTM1, and PINK1 mRNA expression and by Western blot analysis of p62/SQSTM1 and lipidated LC3 accumulation. Our results showed that detrimental effect of APAP on cell viability was suppressed in the presence of HV110 which was linked with increased conversion of LC3 protein and p62/SQSTM1 protein degradation. Additionally, higher p62/SQSTM1 and PINK1 mRNA transcription were noticed in cells co-treated with APAP/HV110, simultaneously. In conclusion, this study suggests that HV110 enhances activation of PINK1-dependent autophagy in HepG2 cells and its eventual co-supplementation with APAP could be potentially used for alleviation of hepatotoxic side effects caused by APAP overdose.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - Lactobacillus fermentum Postbiotic-induced Autophagy as Potential Approach for Treatment of Acetaminophen Hepatotoxicity
VL  - 8
DO  - 10.3389/fmicb.2017.00594
ER  - 

@article{
author = "Dinić, Miroslav and Lukić, Jovanka and Đokić, Jelena and Milenković, Marina and Strahinić, Ivana and Golić, Nataša and Begović, Jelena",
year = "2017",
abstract = "The aim of this study was to investigate the potential of postbiotics originated from Lactobacillus fermentum BGHV110 strain (HV110) to counteract acetaminophen (APAP)-induced hepatotoxicity in HepG2 cells. This strain was selected according to its autophagy inducing potential, based on previous studies reporting protective role of autophagy in APAP caused cellular damage. Cell viability was assessed using MTT and LDH assays, while autophagy was monitored by qPCR analysis of BECN1, Atg5, p62/SQSTM1, and PINK1 mRNA expression and by Western blot analysis of p62/SQSTM1 and lipidated LC3 accumulation. Our results showed that detrimental effect of APAP on cell viability was suppressed in the presence of HV110 which was linked with increased conversion of LC3 protein and p62/SQSTM1 protein degradation. Additionally, higher p62/SQSTM1 and PINK1 mRNA transcription were noticed in cells co-treated with APAP/HV110, simultaneously. In conclusion, this study suggests that HV110 enhances activation of PINK1-dependent autophagy in HepG2 cells and its eventual co-supplementation with APAP could be potentially used for alleviation of hepatotoxic side effects caused by APAP overdose.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "Lactobacillus fermentum Postbiotic-induced Autophagy as Potential Approach for Treatment of Acetaminophen Hepatotoxicity",
volume = "8",
doi = "10.3389/fmicb.2017.00594"
}

Dinić, M., Lukić, J., Đokić, J., Milenković, M., Strahinić, I., Golić, N.,& Begović, J.. (2017). Lactobacillus fermentum Postbiotic-induced Autophagy as Potential Approach for Treatment of Acetaminophen Hepatotoxicity. in Frontiers in Microbiology
Frontiers Media Sa, Lausanne., 8.
https://doi.org/10.3389/fmicb.2017.00594

Dinić M, Lukić J, Đokić J, Milenković M, Strahinić I, Golić N, Begović J. Lactobacillus fermentum Postbiotic-induced Autophagy as Potential Approach for Treatment of Acetaminophen Hepatotoxicity. in Frontiers in Microbiology. 2017;8.
doi:10.3389/fmicb.2017.00594 .

Dinić, Miroslav, Lukić, Jovanka, Đokić, Jelena, Milenković, Marina, Strahinić, Ivana, Golić, Nataša, Begović, Jelena, "Lactobacillus fermentum Postbiotic-induced Autophagy as Potential Approach for Treatment of Acetaminophen Hepatotoxicity" in Frontiers in Microbiology, 8 (2017),
https://doi.org/10.3389/fmicb.2017.00594 . .

TY  - JOUR
AU  - Lukić, Jovanka
AU  - Jančić, Ivan
AU  - Mirković, Nemanja
AU  - Bufan, Biljana
AU  - Đokić, Jelena
AU  - Milenković, Marina
AU  - Begović, J.
AU  - Strahinić, Ivana
AU  - Lozo, J.
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2871
AB  - In the light of the increasing resistance of bacterial pathogens to antibiotics, one of the main global strategies in applied science is development of alternative treatments, which would be safe both for the host and from the environmental perspective. Accordingly, the aim of this study was to test whether two lactic acid bacteria (LAB) strains, Lactococcus lactis BGBU1-4 and Lactobacillus salivarius BGHO1, could be applied as safe supplements for Listeria infection. Two major research objectives were set: to compare the effects of BGBU1-4 and BGHO1 on early immune response in gut tissue of Wistar rats co-administered with Listeria monocytogenes ATCC19111 and next, to test how this applies to their usage as therapeutics in acute ATCC19111 infection. Intestinal villi (IV), Peyer's patches (PP) and mesenteric lymph nodes (MLN) were used for the analysis. The results showed that BGHO1 increased the mRNA expression of innate immune markers CD14, interleukin (IL)-1 beta and tumour necrosis factor (TNF)-alpha in PP and IV, and, in parallel, caused a decrease of listeriolysin O (LLO) mRNA expression in same tissues. In MLN of BGHO1 treated rats, LLO expression was increased, along with an increase of the expression of OX-62 mRNA and CD69, pointing to the activation of adaptive immunity. On the other hand, in BGBU1-4 treated rats, there was no reduction of LLO mRNA expression and no induction of innate immunity markers in intestinal tissue. Additionally, CD14 and IL-1 beta, as well as LLO, but not OX-62 mRNA and CD69 expression, were elevated in MLN of BGBU1-4 treated rats. However, when applied therapeutically, both, BGBU1-4 and BGHO1, lowered Listeria count in spleens of infected rats. Our results not only reveal the potential of LAB to ameliorate Listeria infections, but suggest different immunological effects of two different LAB strains, both of which could be effective in Listeria elimination.
PB  - Wageningen Academic Publishers, Wageningen
T2  - Beneficial Microbes
T1  - Lactococcus lactis and Lactobacillus salivarius differently modulate early immunological response of Wistar rats co-administered with Listeria monocytogenes
VL  - 8
IS  - 5
SP  - 809
EP  - 822
DO  - 10.3920/BM2017.0007
ER  - 

@article{
author = "Lukić, Jovanka and Jančić, Ivan and Mirković, Nemanja and Bufan, Biljana and Đokić, Jelena and Milenković, Marina and Begović, J. and Strahinić, Ivana and Lozo, J.",
year = "2017",
abstract = "In the light of the increasing resistance of bacterial pathogens to antibiotics, one of the main global strategies in applied science is development of alternative treatments, which would be safe both for the host and from the environmental perspective. Accordingly, the aim of this study was to test whether two lactic acid bacteria (LAB) strains, Lactococcus lactis BGBU1-4 and Lactobacillus salivarius BGHO1, could be applied as safe supplements for Listeria infection. Two major research objectives were set: to compare the effects of BGBU1-4 and BGHO1 on early immune response in gut tissue of Wistar rats co-administered with Listeria monocytogenes ATCC19111 and next, to test how this applies to their usage as therapeutics in acute ATCC19111 infection. Intestinal villi (IV), Peyer's patches (PP) and mesenteric lymph nodes (MLN) were used for the analysis. The results showed that BGHO1 increased the mRNA expression of innate immune markers CD14, interleukin (IL)-1 beta and tumour necrosis factor (TNF)-alpha in PP and IV, and, in parallel, caused a decrease of listeriolysin O (LLO) mRNA expression in same tissues. In MLN of BGHO1 treated rats, LLO expression was increased, along with an increase of the expression of OX-62 mRNA and CD69, pointing to the activation of adaptive immunity. On the other hand, in BGBU1-4 treated rats, there was no reduction of LLO mRNA expression and no induction of innate immunity markers in intestinal tissue. Additionally, CD14 and IL-1 beta, as well as LLO, but not OX-62 mRNA and CD69 expression, were elevated in MLN of BGBU1-4 treated rats. However, when applied therapeutically, both, BGBU1-4 and BGHO1, lowered Listeria count in spleens of infected rats. Our results not only reveal the potential of LAB to ameliorate Listeria infections, but suggest different immunological effects of two different LAB strains, both of which could be effective in Listeria elimination.",
publisher = "Wageningen Academic Publishers, Wageningen",
journal = "Beneficial Microbes",
title = "Lactococcus lactis and Lactobacillus salivarius differently modulate early immunological response of Wistar rats co-administered with Listeria monocytogenes",
volume = "8",
number = "5",
pages = "809-822",
doi = "10.3920/BM2017.0007"
}

Lukić, J., Jančić, I., Mirković, N., Bufan, B., Đokić, J., Milenković, M., Begović, J., Strahinić, I.,& Lozo, J.. (2017). Lactococcus lactis and Lactobacillus salivarius differently modulate early immunological response of Wistar rats co-administered with Listeria monocytogenes. in Beneficial Microbes
Wageningen Academic Publishers, Wageningen., 8(5), 809-822.
https://doi.org/10.3920/BM2017.0007

Lukić J, Jančić I, Mirković N, Bufan B, Đokić J, Milenković M, Begović J, Strahinić I, Lozo J. Lactococcus lactis and Lactobacillus salivarius differently modulate early immunological response of Wistar rats co-administered with Listeria monocytogenes. in Beneficial Microbes. 2017;8(5):809-822.
doi:10.3920/BM2017.0007 .

Lukić, Jovanka, Jančić, Ivan, Mirković, Nemanja, Bufan, Biljana, Đokić, Jelena, Milenković, Marina, Begović, J., Strahinić, Ivana, Lozo, J., "Lactococcus lactis and Lactobacillus salivarius differently modulate early immunological response of Wistar rats co-administered with Listeria monocytogenes" in Beneficial Microbes, 8, no. 5 (2017):809-822,
https://doi.org/10.3920/BM2017.0007 . .

TY  - JOUR
AU  - Filipić, Brankica
AU  - Malesević, Milka
AU  - Vasiljević, Zorica
AU  - Lukić, Jovanka
AU  - Novović, Katarina
AU  - Kojić, Milan
AU  - Jovčić, Branko
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2999
AB  - Achromobacter spp. are recognized as emerging pathogens in hospitalized as well as in cystic fibrosis (CF) patients. From 2012 to 2015, we collected 69 clinical isolates (41 patient) of Achromobacter spp. from 13 patients with CF (CF isolates, n = 32) and 28 patients receiving care for other health conditions (non-CF isolates, n = 37). Molecular epidemiology and virulence potential of isolates were examined. Antimicrobial susceptibility, motility, ability to form biofilms and binding affinity to mucin, collagen, and fibronectin were tested to assess their virulence traits. The nrdA gene sequencing showed that A. xylosoxidans was the most prevalent species in both CF and non-CF patients. CF patients were also colonized with A. dolens/A, ruhlandii, A. insuavis, and A. spiritinus strains while non-CF group was somewhat less heterogenous, although A. insuavis, A. insolitus, and A. piechaudii strains were detected beside A. xylosoxidans. Three strains displayed clonal distribution, one among patients from the CF group and two among non-CF patients. No significant differences in susceptibility to antimicrobials were observed between CF and non-CF patients. About one third of the isolates were classified as strong biofilm producers, and the proportion of CF and non-CF isolates with the ability to form biofilm was almost identical. CF isolates were less motile compared to the non-CF group and no correlation was found between swimming phenotype and biofilm formation. On the other hand, CF isolates exhibited higher affinity to bind mucin, collagen, and fibronectin. In generall, CF isolates from our study exhibited in vitro properties that could be of importance for the colonization of CF patients.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Cellular and Infection Microbiology
T1  - Uncovering Differences in Virulence Markers Associated with Achromobacter Species of CF and Non-CF Origin
VL  - 7
DO  - 10.3389/fcimb.2017.00224
ER  - 

@article{
author = "Filipić, Brankica and Malesević, Milka and Vasiljević, Zorica and Lukić, Jovanka and Novović, Katarina and Kojić, Milan and Jovčić, Branko",
year = "2017",
abstract = "Achromobacter spp. are recognized as emerging pathogens in hospitalized as well as in cystic fibrosis (CF) patients. From 2012 to 2015, we collected 69 clinical isolates (41 patient) of Achromobacter spp. from 13 patients with CF (CF isolates, n = 32) and 28 patients receiving care for other health conditions (non-CF isolates, n = 37). Molecular epidemiology and virulence potential of isolates were examined. Antimicrobial susceptibility, motility, ability to form biofilms and binding affinity to mucin, collagen, and fibronectin were tested to assess their virulence traits. The nrdA gene sequencing showed that A. xylosoxidans was the most prevalent species in both CF and non-CF patients. CF patients were also colonized with A. dolens/A, ruhlandii, A. insuavis, and A. spiritinus strains while non-CF group was somewhat less heterogenous, although A. insuavis, A. insolitus, and A. piechaudii strains were detected beside A. xylosoxidans. Three strains displayed clonal distribution, one among patients from the CF group and two among non-CF patients. No significant differences in susceptibility to antimicrobials were observed between CF and non-CF patients. About one third of the isolates were classified as strong biofilm producers, and the proportion of CF and non-CF isolates with the ability to form biofilm was almost identical. CF isolates were less motile compared to the non-CF group and no correlation was found between swimming phenotype and biofilm formation. On the other hand, CF isolates exhibited higher affinity to bind mucin, collagen, and fibronectin. In generall, CF isolates from our study exhibited in vitro properties that could be of importance for the colonization of CF patients.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Cellular and Infection Microbiology",
title = "Uncovering Differences in Virulence Markers Associated with Achromobacter Species of CF and Non-CF Origin",
volume = "7",
doi = "10.3389/fcimb.2017.00224"
}

Filipić, B., Malesević, M., Vasiljević, Z., Lukić, J., Novović, K., Kojić, M.,& Jovčić, B.. (2017). Uncovering Differences in Virulence Markers Associated with Achromobacter Species of CF and Non-CF Origin. in Frontiers in Cellular and Infection Microbiology
Frontiers Media Sa, Lausanne., 7.
https://doi.org/10.3389/fcimb.2017.00224

Filipić B, Malesević M, Vasiljević Z, Lukić J, Novović K, Kojić M, Jovčić B. Uncovering Differences in Virulence Markers Associated with Achromobacter Species of CF and Non-CF Origin. in Frontiers in Cellular and Infection Microbiology. 2017;7.
doi:10.3389/fcimb.2017.00224 .

Filipić, Brankica, Malesević, Milka, Vasiljević, Zorica, Lukić, Jovanka, Novović, Katarina, Kojić, Milan, Jovčić, Branko, "Uncovering Differences in Virulence Markers Associated with Achromobacter Species of CF and Non-CF Origin" in Frontiers in Cellular and Infection Microbiology, 7 (2017),
https://doi.org/10.3389/fcimb.2017.00224 . .

TY  - JOUR
AU  - Malesević, Milka
AU  - Vasiljević, Zorica
AU  - Sovtić, Aleksandar
AU  - Filipić, Brankica
AU  - Novović, Katarina
AU  - Kojić, Milan
AU  - Jovčić, Branko
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2974
AB  - Background: Burkholderia cenocepacia is considered one of the most problematic cystic fibrosis (CF) pathogens. Colonization prevalence in the Serbian CF population is high and virtually exclusively limited to a single highly transmissible clone of B. cenocepacia ST856 which is positive for both the B. cepacia epidemic strain marker (BCESM) and cable pilin, and is closely related to the epidemic strain CZ1 (ST32). Methods: Biofilm formation for 182 isolates, and adhesion to components of the host extracellular matrix, proteolytic activity, mucoidy and motility of selected ST856 representatives, as well as B. cenocepacia ST858 and ST859, and B. stabilis ST857, novel STs isolated from Serbian CF patients, were investigated in this study. The presence of the cepI, cepR, fliG, llpE, wbiI, and bcscV genes was analyzed. Results: Biofilm-formation ability of analyzed strains was poor under standard laboratory conditions, but changed in stress conditions (cold stress) and conditions that mimic CF milieu (increased CO2). All strains expressed ability to bind to collagen and fibronectin albeit with different intensity. Representatives of ST856 exhibited gelatinase activity. ST858, ST859 and 9/11 of ST856 genotypes were positive for swimming and twitching motility whereas ST857 was non-motile. Mucoidy was demonstrated in all ST856 genotypes, ST857 was semi-mucoid, and ST858 and ST859 were non-mucoid. Molecular analysis for major virulence factors revealed that ST856 and ST857 carried the six analyzed genes, while ST858 and ST859 were negative for the llpE gene. Conclusion: Variations in virulence phenotypes in different genotypes of epidemic B. cenocepacia ST856 clone, in vitro, could be a consequence of diversification driven by pathoadaptation. Diversity of epidemic clone genotypes virulence, could be challenging for accurate diagnosis and treatment, as well as for infection control.
PB  - Biomed Central Ltd, London
T2  - Antimicrobial Resistance and Infection Control
T1  - Virulence traits associated with Burkholderia cenocepacia ST856 epidemic strain isolated from cystic fibrosis patients
VL  - 6
DO  - 10.1186/s13756-017-0215-y
ER  - 

@article{
author = "Malesević, Milka and Vasiljević, Zorica and Sovtić, Aleksandar and Filipić, Brankica and Novović, Katarina and Kojić, Milan and Jovčić, Branko",
year = "2017",
abstract = "Background: Burkholderia cenocepacia is considered one of the most problematic cystic fibrosis (CF) pathogens. Colonization prevalence in the Serbian CF population is high and virtually exclusively limited to a single highly transmissible clone of B. cenocepacia ST856 which is positive for both the B. cepacia epidemic strain marker (BCESM) and cable pilin, and is closely related to the epidemic strain CZ1 (ST32). Methods: Biofilm formation for 182 isolates, and adhesion to components of the host extracellular matrix, proteolytic activity, mucoidy and motility of selected ST856 representatives, as well as B. cenocepacia ST858 and ST859, and B. stabilis ST857, novel STs isolated from Serbian CF patients, were investigated in this study. The presence of the cepI, cepR, fliG, llpE, wbiI, and bcscV genes was analyzed. Results: Biofilm-formation ability of analyzed strains was poor under standard laboratory conditions, but changed in stress conditions (cold stress) and conditions that mimic CF milieu (increased CO2). All strains expressed ability to bind to collagen and fibronectin albeit with different intensity. Representatives of ST856 exhibited gelatinase activity. ST858, ST859 and 9/11 of ST856 genotypes were positive for swimming and twitching motility whereas ST857 was non-motile. Mucoidy was demonstrated in all ST856 genotypes, ST857 was semi-mucoid, and ST858 and ST859 were non-mucoid. Molecular analysis for major virulence factors revealed that ST856 and ST857 carried the six analyzed genes, while ST858 and ST859 were negative for the llpE gene. Conclusion: Variations in virulence phenotypes in different genotypes of epidemic B. cenocepacia ST856 clone, in vitro, could be a consequence of diversification driven by pathoadaptation. Diversity of epidemic clone genotypes virulence, could be challenging for accurate diagnosis and treatment, as well as for infection control.",
publisher = "Biomed Central Ltd, London",
journal = "Antimicrobial Resistance and Infection Control",
title = "Virulence traits associated with Burkholderia cenocepacia ST856 epidemic strain isolated from cystic fibrosis patients",
volume = "6",
doi = "10.1186/s13756-017-0215-y"
}

Malesević, M., Vasiljević, Z., Sovtić, A., Filipić, B., Novović, K., Kojić, M.,& Jovčić, B.. (2017). Virulence traits associated with Burkholderia cenocepacia ST856 epidemic strain isolated from cystic fibrosis patients. in Antimicrobial Resistance and Infection Control
Biomed Central Ltd, London., 6.
https://doi.org/10.1186/s13756-017-0215-y

Malesević M, Vasiljević Z, Sovtić A, Filipić B, Novović K, Kojić M, Jovčić B. Virulence traits associated with Burkholderia cenocepacia ST856 epidemic strain isolated from cystic fibrosis patients. in Antimicrobial Resistance and Infection Control. 2017;6.
doi:10.1186/s13756-017-0215-y .

Malesević, Milka, Vasiljević, Zorica, Sovtić, Aleksandar, Filipić, Brankica, Novović, Katarina, Kojić, Milan, Jovčić, Branko, "Virulence traits associated with Burkholderia cenocepacia ST856 epidemic strain isolated from cystic fibrosis patients" in Antimicrobial Resistance and Infection Control, 6 (2017),
https://doi.org/10.1186/s13756-017-0215-y . .

TY  - JOUR
AU  - Lukić, Jovanka
AU  - Dinić, Miroslav
AU  - Marčetić, Mirjana
AU  - Milenković, Marina
AU  - Begović, Jelena
AU  - Strahinić, Ivana
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2645
AB  - Aim of this work was to assess the metabolic and physiological changes that occurred in the hind gut of rats after feeding with soybean carbohydrates alone and in combination with Lactobacillus helveticus BGRA43. Wistar rats were gavaged with soybean flour for 28 days. The parameters assessed included fecal volatile organic compounds, and L-lactate, reducing sugars, proteins, ammonia and water levels in the colonic lumen. The presence of lactic acid (LAB), sulfate reducing (SRB) and methanogenic bacteria was assessed by semi-quantitative PCR. Malondialdehyde levels as well as lymphoid tissue size in ileal and colonic mucosa were also evaluated. On the basics of the results obtained, correlation network was created, setting the parameters tested in research in two metabolic groups: saccharolytic and proteolytic fermentation group. The principal finding of the study is a negative correlation between oral administration of BGRA43 and increase of parameters related to carbohydrate fermentation in the gut, and a positive correlation to factors related to proteolytic fermentation. On the contrary, soybean carbohydrates were correlated with increased values of factors related to carbohydrate catabolism. Different effects of BGRA43 and soybean carbohydrates on metabolic processes in colonic lumen indicate the possibility of applying the BGRA43 in alleviating the gastrointestinal symptoms occurring after consuming hardly digestible carbohydrates.
PB  - Društvo genetičara Srbije, Beograd
T2  - Genetika, Belgrade
T1  - Effects of soybean carbohydrates and Lactobacillus helveticus BGRA43 on metabolic processes in rat colon
VL  - 48
IS  - 3
SP  - 903
EP  - 921
DO  - 10.2298/GENSR1603903L
ER  - 

@article{
author = "Lukić, Jovanka and Dinić, Miroslav and Marčetić, Mirjana and Milenković, Marina and Begović, Jelena and Strahinić, Ivana",
year = "2016",
abstract = "Aim of this work was to assess the metabolic and physiological changes that occurred in the hind gut of rats after feeding with soybean carbohydrates alone and in combination with Lactobacillus helveticus BGRA43. Wistar rats were gavaged with soybean flour for 28 days. The parameters assessed included fecal volatile organic compounds, and L-lactate, reducing sugars, proteins, ammonia and water levels in the colonic lumen. The presence of lactic acid (LAB), sulfate reducing (SRB) and methanogenic bacteria was assessed by semi-quantitative PCR. Malondialdehyde levels as well as lymphoid tissue size in ileal and colonic mucosa were also evaluated. On the basics of the results obtained, correlation network was created, setting the parameters tested in research in two metabolic groups: saccharolytic and proteolytic fermentation group. The principal finding of the study is a negative correlation between oral administration of BGRA43 and increase of parameters related to carbohydrate fermentation in the gut, and a positive correlation to factors related to proteolytic fermentation. On the contrary, soybean carbohydrates were correlated with increased values of factors related to carbohydrate catabolism. Different effects of BGRA43 and soybean carbohydrates on metabolic processes in colonic lumen indicate the possibility of applying the BGRA43 in alleviating the gastrointestinal symptoms occurring after consuming hardly digestible carbohydrates.",
publisher = "Društvo genetičara Srbije, Beograd",
journal = "Genetika, Belgrade",
title = "Effects of soybean carbohydrates and Lactobacillus helveticus BGRA43 on metabolic processes in rat colon",
volume = "48",
number = "3",
pages = "903-921",
doi = "10.2298/GENSR1603903L"
}

Lukić, J., Dinić, M., Marčetić, M., Milenković, M., Begović, J.,& Strahinić, I.. (2016). Effects of soybean carbohydrates and Lactobacillus helveticus BGRA43 on metabolic processes in rat colon. in Genetika, Belgrade
Društvo genetičara Srbije, Beograd., 48(3), 903-921.
https://doi.org/10.2298/GENSR1603903L

Lukić J, Dinić M, Marčetić M, Milenković M, Begović J, Strahinić I. Effects of soybean carbohydrates and Lactobacillus helveticus BGRA43 on metabolic processes in rat colon. in Genetika, Belgrade. 2016;48(3):903-921.
doi:10.2298/GENSR1603903L .

Lukić, Jovanka, Dinić, Miroslav, Marčetić, Mirjana, Milenković, Marina, Begović, Jelena, Strahinić, Ivana, "Effects of soybean carbohydrates and Lactobacillus helveticus BGRA43 on metabolic processes in rat colon" in Genetika, Belgrade, 48, no. 3 (2016):903-921,
https://doi.org/10.2298/GENSR1603903L . .

TY  - JOUR
AU  - Terzić-Vidojević, Amarela
AU  - Veljović, Katarina
AU  - Begović, Jelena
AU  - Filipić, Brankica
AU  - Popović, Dušanka
AU  - Tolinacki, Maja
AU  - Miljković, Marija
AU  - Kojić, Milan
AU  - Golić, Nataša
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2320
AB  - Enterococci represent the most controversial group of dairy bacteria. They are found to be the main constituent of many traditional Mediterranean dairy products and contribute to their characteristic taste and flavor. On the other hand, during the last 50 years antibiotic resistant enterococci have emerged as leading causes of nosocomial infections worldwide. The aim of this study was to determine the diversity, technological properties, antibiotic susceptibility and virulence traits of 636 enterococci previously isolated from 55 artisan dairy products from 12 locations in the Western Balkan countries (WBC) of Serbia, Croatia and Bosnia and Herzegovina. All strains were identified both by microbiological and molecular methods. The predominant species was Enterococcus durans, followed by Enterococcus faecalis and Enterococcus faecium. Over 44% of the isolates were resistant to ciprofloxacin and erythromycin, while 26.2% of the isolates were multi resistant to three or more antibiotics belonging to different families. 185 isolates (29.1%) were susceptible to all 13 of the antibiotics tested. The antibiotic-susceptible isolates were further tested for possible virulence genes and the production of biogenic amines. Finally, five enterococci isolates were found to be antibiotic susceptible with good technological characteristics and without virulence traits or the ability to produce biogenic amines, making them possible candidates for biotechnological application as starter cultures in the dairy industry.
PB  - Frontiers Media Sa, Lausanne
T2  - Frontiers in Microbiology
T1  - Diversity and antibiotic susceptibility of autochthonous dairy enterococci isolates: are they safe candidates for autochthonous starter cultures?
VL  - 6
DO  - 10.3389/fmicb.2015.00954
ER  - 

@article{
author = "Terzić-Vidojević, Amarela and Veljović, Katarina and Begović, Jelena and Filipić, Brankica and Popović, Dušanka and Tolinacki, Maja and Miljković, Marija and Kojić, Milan and Golić, Nataša",
year = "2015",
abstract = "Enterococci represent the most controversial group of dairy bacteria. They are found to be the main constituent of many traditional Mediterranean dairy products and contribute to their characteristic taste and flavor. On the other hand, during the last 50 years antibiotic resistant enterococci have emerged as leading causes of nosocomial infections worldwide. The aim of this study was to determine the diversity, technological properties, antibiotic susceptibility and virulence traits of 636 enterococci previously isolated from 55 artisan dairy products from 12 locations in the Western Balkan countries (WBC) of Serbia, Croatia and Bosnia and Herzegovina. All strains were identified both by microbiological and molecular methods. The predominant species was Enterococcus durans, followed by Enterococcus faecalis and Enterococcus faecium. Over 44% of the isolates were resistant to ciprofloxacin and erythromycin, while 26.2% of the isolates were multi resistant to three or more antibiotics belonging to different families. 185 isolates (29.1%) were susceptible to all 13 of the antibiotics tested. The antibiotic-susceptible isolates were further tested for possible virulence genes and the production of biogenic amines. Finally, five enterococci isolates were found to be antibiotic susceptible with good technological characteristics and without virulence traits or the ability to produce biogenic amines, making them possible candidates for biotechnological application as starter cultures in the dairy industry.",
publisher = "Frontiers Media Sa, Lausanne",
journal = "Frontiers in Microbiology",
title = "Diversity and antibiotic susceptibility of autochthonous dairy enterococci isolates: are they safe candidates for autochthonous starter cultures?",
volume = "6",
doi = "10.3389/fmicb.2015.00954"
}

Terzić-Vidojević, A., Veljović, K., Begović, J., Filipić, B., Popović, D., Tolinacki, M., Miljković, M., Kojić, M.,& Golić, N.. (2015). Diversity and antibiotic susceptibility of autochthonous dairy enterococci isolates: are they safe candidates for autochthonous starter cultures?. in Frontiers in Microbiology
Frontiers Media Sa, Lausanne., 6.
https://doi.org/10.3389/fmicb.2015.00954

Terzić-Vidojević A, Veljović K, Begović J, Filipić B, Popović D, Tolinacki M, Miljković M, Kojić M, Golić N. Diversity and antibiotic susceptibility of autochthonous dairy enterococci isolates: are they safe candidates for autochthonous starter cultures?. in Frontiers in Microbiology. 2015;6.
doi:10.3389/fmicb.2015.00954 .

Terzić-Vidojević, Amarela, Veljović, Katarina, Begović, Jelena, Filipić, Brankica, Popović, Dušanka, Tolinacki, Maja, Miljković, Marija, Kojić, Milan, Golić, Nataša, "Diversity and antibiotic susceptibility of autochthonous dairy enterococci isolates: are they safe candidates for autochthonous starter cultures?" in Frontiers in Microbiology, 6 (2015),
https://doi.org/10.3389/fmicb.2015.00954 . .

TY  - JOUR
AU  - Novović, Katarina
AU  - Mihajlović, Sanja
AU  - Vasiljević, Zorica
AU  - Filipić, Brankica
AU  - Begović, Jelena
AU  - Jovčić, Branko
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2421
AB  - Carbapenem-resistant A. baumannii present a significant therapeutic challenge for the treatment of nosocomial infections in many European countries. Although it is known that the gradient of A. baumannii prevalence increases from northern to southern Europe, this study provides the first data from Serbia. Twenty-eight carbapenem-resistant A. baumannii clinical isolates were collected at a Serbian pediatric hospital during a 2-year period. The majority of isolates (67.68%) belonged to the sequence type Group 1, European clonal complex II. All isolates harbored intrinsic OXA-51 and AmpC cephalosporinase. OXA-23 was detected in 16 isolates (57.14%), OXA-24 in 23 isolates (82.14%) and OXA-58 in 11 isolates (39.29%). Six of the isolates (21.43%) harbored all of the analyzed oxacillinases, except OXA-143 and OXA-235 that were not detected in this study. Production of oxacillinases was detected in different pulsotypes indicating the presence of horizontal gene transfer. NDM-1, VIM and IMP were not detected in analyzed clinical A. baumannii isolates. ISAba1 insertion sequence was present upstream of OXA-51 in one isolate, upstream of AmpC in 13 isolates and upstream of OXA-23 in 10 isolates. In silico analysis of carO sequences from analyzed A. baumannii isolates revealed the existence of two out of six highly polymorphic CarO variants. The phylogenetic analysis of CarO protein among Acinetobacter species revised the previous classification CarO variants into three groups based on strong bootstraps scores in the tree analysis. Group I comprises four variants (I-IV) while Groups II and III contain only one variant each. One half of the Serbian clinical isolates belong to Group I variant I, while the other half belongs to Group I variant III.
PB  - Public Library Science, San Francisco
T2  - PLoS One
T1  - Carbapenem-Resistant Acinetobacter baumannii from Serbia: Revision of CarO Classification
VL  - 10
IS  - 3
DO  - 10.1371/journal.pone.0122793
ER  - 

@article{
author = "Novović, Katarina and Mihajlović, Sanja and Vasiljević, Zorica and Filipić, Brankica and Begović, Jelena and Jovčić, Branko",
year = "2015",
abstract = "Carbapenem-resistant A. baumannii present a significant therapeutic challenge for the treatment of nosocomial infections in many European countries. Although it is known that the gradient of A. baumannii prevalence increases from northern to southern Europe, this study provides the first data from Serbia. Twenty-eight carbapenem-resistant A. baumannii clinical isolates were collected at a Serbian pediatric hospital during a 2-year period. The majority of isolates (67.68%) belonged to the sequence type Group 1, European clonal complex II. All isolates harbored intrinsic OXA-51 and AmpC cephalosporinase. OXA-23 was detected in 16 isolates (57.14%), OXA-24 in 23 isolates (82.14%) and OXA-58 in 11 isolates (39.29%). Six of the isolates (21.43%) harbored all of the analyzed oxacillinases, except OXA-143 and OXA-235 that were not detected in this study. Production of oxacillinases was detected in different pulsotypes indicating the presence of horizontal gene transfer. NDM-1, VIM and IMP were not detected in analyzed clinical A. baumannii isolates. ISAba1 insertion sequence was present upstream of OXA-51 in one isolate, upstream of AmpC in 13 isolates and upstream of OXA-23 in 10 isolates. In silico analysis of carO sequences from analyzed A. baumannii isolates revealed the existence of two out of six highly polymorphic CarO variants. The phylogenetic analysis of CarO protein among Acinetobacter species revised the previous classification CarO variants into three groups based on strong bootstraps scores in the tree analysis. Group I comprises four variants (I-IV) while Groups II and III contain only one variant each. One half of the Serbian clinical isolates belong to Group I variant I, while the other half belongs to Group I variant III.",
publisher = "Public Library Science, San Francisco",
journal = "PLoS One",
title = "Carbapenem-Resistant Acinetobacter baumannii from Serbia: Revision of CarO Classification",
volume = "10",
number = "3",
doi = "10.1371/journal.pone.0122793"
}

Novović, K., Mihajlović, S., Vasiljević, Z., Filipić, B., Begović, J.,& Jovčić, B.. (2015). Carbapenem-Resistant Acinetobacter baumannii from Serbia: Revision of CarO Classification. in PLoS One
Public Library Science, San Francisco., 10(3).
https://doi.org/10.1371/journal.pone.0122793

Novović K, Mihajlović S, Vasiljević Z, Filipić B, Begović J, Jovčić B. Carbapenem-Resistant Acinetobacter baumannii from Serbia: Revision of CarO Classification. in PLoS One. 2015;10(3).
doi:10.1371/journal.pone.0122793 .

Novović, Katarina, Mihajlović, Sanja, Vasiljević, Zorica, Filipić, Brankica, Begović, Jelena, Jovčić, Branko, "Carbapenem-Resistant Acinetobacter baumannii from Serbia: Revision of CarO Classification" in PLoS One, 10, no. 3 (2015),
https://doi.org/10.1371/journal.pone.0122793 . .

TY  - JOUR
AU  - Novović, Katarina
AU  - Filipić, Brankica
AU  - Veljović, Katarina
AU  - Begović, J.
AU  - Mirković, Nemanja
AU  - Jovčić, Branko
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2388
AB  - New Delhi metallo-beta-lactamase-1 (NDM-1) will soon become the most commonly isolated and distributed metallo-beta-lactamase worldwide due to its rapid international dissemination and its ability to be expressed by numerous Gram-negative pathogens. NDM-positive bacteria pose a significant public health threat in the Indian subcontinent and the Balkans, which have been designated as endemic regions. Our study was focused on urban rivers, a lake and springheads as a potential source of NDM-1-producing strains in Serbia, but also as a source of other metallo-beta-lactamases and extended-spectrum beta-lactamase (ESBL) producing bacteria. A total of 69 beta-lactam resistant isolates, belonging to 12 bacterial genera, were collected from 8 out of 10 different locations in Belgrade, of which the most were from a popular recreational site, Ada Ciganlija Lake. Phenotypic tests revealed 7 (10.14%) ESBL-producing isolates and 39 (56.52%) isolates resistant to imipenem, of which 32 were positive for metallo-beta-lactamase (MBL) production. PCR and sequencing revealed the presence of genetic determinants for SHV (3 isolates), DHA-1 (1 isolate) and CMY-2 (1 isolate) beta-lactamases. However, we did not detect any NDM-1-producing strains (previously described cases of NDM-1 from Serbia were limited to Belgrade), so we propose that Serbian NDM-1 is in fact a transplant and a nosocomial, rather than an environmental, issue and that Serbia is not an endemic region for NDM-1.
PB  - Elsevier Science BV, Amsterdam
T2  - Scientia Horticulturae
T1  - Environmental waters and bla(NDm-1) in Belgrade, Serbia: Endemicity questioned
VL  - 511
SP  - 393
EP  - 398
DO  - 10.1016/j.scitotenv.2014.12.072
ER  - 

@article{
author = "Novović, Katarina and Filipić, Brankica and Veljović, Katarina and Begović, J. and Mirković, Nemanja and Jovčić, Branko",
year = "2015",
abstract = "New Delhi metallo-beta-lactamase-1 (NDM-1) will soon become the most commonly isolated and distributed metallo-beta-lactamase worldwide due to its rapid international dissemination and its ability to be expressed by numerous Gram-negative pathogens. NDM-positive bacteria pose a significant public health threat in the Indian subcontinent and the Balkans, which have been designated as endemic regions. Our study was focused on urban rivers, a lake and springheads as a potential source of NDM-1-producing strains in Serbia, but also as a source of other metallo-beta-lactamases and extended-spectrum beta-lactamase (ESBL) producing bacteria. A total of 69 beta-lactam resistant isolates, belonging to 12 bacterial genera, were collected from 8 out of 10 different locations in Belgrade, of which the most were from a popular recreational site, Ada Ciganlija Lake. Phenotypic tests revealed 7 (10.14%) ESBL-producing isolates and 39 (56.52%) isolates resistant to imipenem, of which 32 were positive for metallo-beta-lactamase (MBL) production. PCR and sequencing revealed the presence of genetic determinants for SHV (3 isolates), DHA-1 (1 isolate) and CMY-2 (1 isolate) beta-lactamases. However, we did not detect any NDM-1-producing strains (previously described cases of NDM-1 from Serbia were limited to Belgrade), so we propose that Serbian NDM-1 is in fact a transplant and a nosocomial, rather than an environmental, issue and that Serbia is not an endemic region for NDM-1.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Scientia Horticulturae",
title = "Environmental waters and bla(NDm-1) in Belgrade, Serbia: Endemicity questioned",
volume = "511",
pages = "393-398",
doi = "10.1016/j.scitotenv.2014.12.072"
}

Novović, K., Filipić, B., Veljović, K., Begović, J., Mirković, N.,& Jovčić, B.. (2015). Environmental waters and bla(NDm-1) in Belgrade, Serbia: Endemicity questioned. in Scientia Horticulturae
Elsevier Science BV, Amsterdam., 511, 393-398.
https://doi.org/10.1016/j.scitotenv.2014.12.072

Novović K, Filipić B, Veljović K, Begović J, Mirković N, Jovčić B. Environmental waters and bla(NDm-1) in Belgrade, Serbia: Endemicity questioned. in Scientia Horticulturae. 2015;511:393-398.
doi:10.1016/j.scitotenv.2014.12.072 .

Novović, Katarina, Filipić, Brankica, Veljović, Katarina, Begović, J., Mirković, Nemanja, Jovčić, Branko, "Environmental waters and bla(NDm-1) in Belgrade, Serbia: Endemicity questioned" in Scientia Horticulturae, 511 (2015):393-398,
https://doi.org/10.1016/j.scitotenv.2014.12.072 . .

TY  - JOUR
AU  - Filipić, Brankica
AU  - Golić, Nataša
AU  - Jovčić, Branko
AU  - Ćupić-Miladinović, Dejana
AU  - Soković, Svetlana
AU  - Popović, Dušanka
AU  - Kojić, Milan
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2483
AB  - Lactic acid bacteria (LAB) are widely used in the food industry, especially in the pro­duction of fermented dairy products and meat. The most studied species among LAB is Lactococcus lactis. L. lactis strains are of great importance in the production of fermented dairy products such as yogurt, butter, fresh cheese and some kind of semi-hard cheese. Although L. lactis acquired the 'Generally Regarded As Safe' (GRAS) status, many investigations indicated that lactococci may act as reservoirs of antibiotic resistance genes, which could be transferred to other bacterial species in human gastrointestinal tract includ­ing pathogens. The genome analysis of L. lactis indicated the presence of at least 40 putative drug transporter genes, and only four multidrug resistance (MDR) transporters are functional­ly characterized: LmrA, LmrP, LmrCD i CmbT. LmrA is the first described MDR transport­er in prokaryotes. LmrCD is responsible for resistance to cholate, which is an integral part of human bile and LmrCD is important for intestinal survival of lactococci that are used as probiotics. Secondary multidrug transporter LmrP confers resistance to lincosamides, mac­rolides, streptogramins and tetracyclines. CmbT protein has an effect on the host cell re­sistance to lincomycin, sulfadiazine, streptomycin, rifampicin, puromycin and sulfametox­azole. Since the food chain is an important way of transmitting resistance genes in human and animal population, it is of great importance to study the mechanisms of resistance in lactococci and other LAB, intended for the food industry.
AB  - Bakterije mlečne kiseline (BMK) imaju veliku primenu u prehrambenoj industriji, posebno u proizvodnji fermentisanih mlečnih proizvoda i mesa. Najviše izučavana vrsta u okviru BMK je Lactococcus lactis. Sojevi koji pripadaju vrsti L. lactis su od izuzetnog značaja u proizvodnji fermentisanih mlečnih proizvoda poput kiselog mleka, maslaca, svežih sireva i nekih vrsta polutvrdih sireva. Iako se generalno laktokoke smatraju bezbednim mikroorganizmima, istraživanja ukazuju da mogu biti rezervoari gena za rezistenciju na antibiotike, koji se horizontalnim transferom mogu preneti na druge bakterijske vrste, uključujući i patogene. Pokazano je da se u membrani bakterija vrste L. lactis nalaze efluksne pumpe koje obezbeđuju rezistenciju na strukturno različite supstrate (eng. multidrug resistance transporters-MDR). Analizom genoma L. lactis detektovano je prisustvo 40 potencijalnih gena koji kodiraju MDR transportere, od čega su do danas svega četiri funkcionalno okarakterisana: LmrA, LmrP, LmrCD i CmbT. LmrA je prvi uopšte opisan primarni transporter kod prokariota. LmrCD obezbeđuje rezistenciju na holat koji ulazi u sastav žuči, što je značajno za preživljavanje laktokoka koje se primenjuju kao probiotici u digestivnom traktu ljudi i životinja. LmrP je sekundarni transporter i obezbeđuje rezistenciju na neke linkozamide, makrolide, streptogramine I tetracikline. CmbT je poslednji okarakterisan MDR transporter kod L. lactis i doprinosi rezistenciji na linkomicin, sulfonamidne antibiotike, sulfametoksazol i sulfadiazin, aminoglikozidni antibiotik streptomicin, aminonukleozidni antibiotik puromicin i semisintetski antibiotik rifampicin. Kako lanac hrane predstavlja važan put prenosa gena za rezistenciju u okviru ljudske i životinjske populacije, neophodno je intenzivno izučavanje mehanizama rezistencije kod laktokoka i drugih BMK namenjenih za upotrebu u prehrambenoj industriji.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski glasnik
T1  - Resistance to antibiotics in lacid acid bacteria: Strain Lactococcus
T1  - Rezistencija na antibiotike kod bakterija mlečne kiseline - rod Lactococcus
VL  - 69
IS  - 3-4
SP  - 271
EP  - 281
DO  - 10.2298/VETGL1504271F
ER  - 

@article{
author = "Filipić, Brankica and Golić, Nataša and Jovčić, Branko and Ćupić-Miladinović, Dejana and Soković, Svetlana and Popović, Dušanka and Kojić, Milan",
year = "2015",
abstract = "Lactic acid bacteria (LAB) are widely used in the food industry, especially in the pro­duction of fermented dairy products and meat. The most studied species among LAB is Lactococcus lactis. L. lactis strains are of great importance in the production of fermented dairy products such as yogurt, butter, fresh cheese and some kind of semi-hard cheese. Although L. lactis acquired the 'Generally Regarded As Safe' (GRAS) status, many investigations indicated that lactococci may act as reservoirs of antibiotic resistance genes, which could be transferred to other bacterial species in human gastrointestinal tract includ­ing pathogens. The genome analysis of L. lactis indicated the presence of at least 40 putative drug transporter genes, and only four multidrug resistance (MDR) transporters are functional­ly characterized: LmrA, LmrP, LmrCD i CmbT. LmrA is the first described MDR transport­er in prokaryotes. LmrCD is responsible for resistance to cholate, which is an integral part of human bile and LmrCD is important for intestinal survival of lactococci that are used as probiotics. Secondary multidrug transporter LmrP confers resistance to lincosamides, mac­rolides, streptogramins and tetracyclines. CmbT protein has an effect on the host cell re­sistance to lincomycin, sulfadiazine, streptomycin, rifampicin, puromycin and sulfametox­azole. Since the food chain is an important way of transmitting resistance genes in human and animal population, it is of great importance to study the mechanisms of resistance in lactococci and other LAB, intended for the food industry., Bakterije mlečne kiseline (BMK) imaju veliku primenu u prehrambenoj industriji, posebno u proizvodnji fermentisanih mlečnih proizvoda i mesa. Najviše izučavana vrsta u okviru BMK je Lactococcus lactis. Sojevi koji pripadaju vrsti L. lactis su od izuzetnog značaja u proizvodnji fermentisanih mlečnih proizvoda poput kiselog mleka, maslaca, svežih sireva i nekih vrsta polutvrdih sireva. Iako se generalno laktokoke smatraju bezbednim mikroorganizmima, istraživanja ukazuju da mogu biti rezervoari gena za rezistenciju na antibiotike, koji se horizontalnim transferom mogu preneti na druge bakterijske vrste, uključujući i patogene. Pokazano je da se u membrani bakterija vrste L. lactis nalaze efluksne pumpe koje obezbeđuju rezistenciju na strukturno različite supstrate (eng. multidrug resistance transporters-MDR). Analizom genoma L. lactis detektovano je prisustvo 40 potencijalnih gena koji kodiraju MDR transportere, od čega su do danas svega četiri funkcionalno okarakterisana: LmrA, LmrP, LmrCD i CmbT. LmrA je prvi uopšte opisan primarni transporter kod prokariota. LmrCD obezbeđuje rezistenciju na holat koji ulazi u sastav žuči, što je značajno za preživljavanje laktokoka koje se primenjuju kao probiotici u digestivnom traktu ljudi i životinja. LmrP je sekundarni transporter i obezbeđuje rezistenciju na neke linkozamide, makrolide, streptogramine I tetracikline. CmbT je poslednji okarakterisan MDR transporter kod L. lactis i doprinosi rezistenciji na linkomicin, sulfonamidne antibiotike, sulfametoksazol i sulfadiazin, aminoglikozidni antibiotik streptomicin, aminonukleozidni antibiotik puromicin i semisintetski antibiotik rifampicin. Kako lanac hrane predstavlja važan put prenosa gena za rezistenciju u okviru ljudske i životinjske populacije, neophodno je intenzivno izučavanje mehanizama rezistencije kod laktokoka i drugih BMK namenjenih za upotrebu u prehrambenoj industriji.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski glasnik",
title = "Resistance to antibiotics in lacid acid bacteria: Strain Lactococcus, Rezistencija na antibiotike kod bakterija mlečne kiseline - rod Lactococcus",
volume = "69",
number = "3-4",
pages = "271-281",
doi = "10.2298/VETGL1504271F"
}

Filipić, B., Golić, N., Jovčić, B., Ćupić-Miladinović, D., Soković, S., Popović, D.,& Kojić, M.. (2015). Resistance to antibiotics in lacid acid bacteria: Strain Lactococcus. in Veterinarski glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 69(3-4), 271-281.
https://doi.org/10.2298/VETGL1504271F

Filipić B, Golić N, Jovčić B, Ćupić-Miladinović D, Soković S, Popović D, Kojić M. Resistance to antibiotics in lacid acid bacteria: Strain Lactococcus. in Veterinarski glasnik. 2015;69(3-4):271-281.
doi:10.2298/VETGL1504271F .

Filipić, Brankica, Golić, Nataša, Jovčić, Branko, Ćupić-Miladinović, Dejana, Soković, Svetlana, Popović, Dušanka, Kojić, Milan, "Resistance to antibiotics in lacid acid bacteria: Strain Lactococcus" in Veterinarski glasnik, 69, no. 3-4 (2015):271-281,
https://doi.org/10.2298/VETGL1504271F . .

TY  - JOUR
AU  - Filipić, Brankica
AU  - Nikolić, Katarina
AU  - Filipić, Slavica
AU  - Jovčić, Branko
AU  - Agbaba, Danica
AU  - Antić-Stanković, Jelena
AU  - Kojić, Milan
AU  - Golić, Nataša
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2232
AB  - The CmbT substrate specificity and its role as a proton motive force-driven drug efflux pump at a molecular level were investigated in the study. In that order, 3D-quantitative structure-activity relationship (3D-QSAR) study was applied for selection of molecular determinants of multidrug recognition by CmbT. CmbT multidrug resistance protein of Lactococcus lactis contributes to extruding the structurally, chemically, and pharmacologically diverse range of substrates out of bacterial cells. This function of CmbT may result in the failure of antibiotic therapy. Homology model of CmbT protein was constructed and further opthnized. The 3D-QSAR model predictive potential was proved by use of leave-one-out cross validation Of the training set (Q(2): 0.69, R-observd(2) (vs).(Predicted) : 0.918, RMSEE: 0.193) and verification set (R-Observed vs predicted(2) : 0.704, RMSEP: 0.289). The results obtained in this study showed that high CmbT affinities to ethidium, sulbactam, and sulfathiazole could be related to the absence of significant unfavourable interactions. In contrast, the presence of specific unfavourable interaction between two hydrogen bond donor groups in bacitracin, apramycin, novobiocin, vancomycin, kanamycin, gentamycin, and tobramycin is found to be the main reason for their lower CmbT affinities. In addition, membrane position of the CmbT binding site and positive correlation between substrates lipophilicity (log D-PH so) and CmbT affinity strongly indicates that CmbT recognizes its substrates within the membrane.
PB  - Elsevier Science BV, Amsterdam
T2  - Journal of the Taiwan Institute of Chemical Engineers
T1  - Identifying the CmbT substrates specificity by using a quantitative structure-activity relationship (QSAR) study
VL  - 45
IS  - 3
SP  - 764
EP  - 771
DO  - 10.1016/j.jtice.2013.09.033
ER  - 

@article{
author = "Filipić, Brankica and Nikolić, Katarina and Filipić, Slavica and Jovčić, Branko and Agbaba, Danica and Antić-Stanković, Jelena and Kojić, Milan and Golić, Nataša",
year = "2014",
abstract = "The CmbT substrate specificity and its role as a proton motive force-driven drug efflux pump at a molecular level were investigated in the study. In that order, 3D-quantitative structure-activity relationship (3D-QSAR) study was applied for selection of molecular determinants of multidrug recognition by CmbT. CmbT multidrug resistance protein of Lactococcus lactis contributes to extruding the structurally, chemically, and pharmacologically diverse range of substrates out of bacterial cells. This function of CmbT may result in the failure of antibiotic therapy. Homology model of CmbT protein was constructed and further opthnized. The 3D-QSAR model predictive potential was proved by use of leave-one-out cross validation Of the training set (Q(2): 0.69, R-observd(2) (vs).(Predicted) : 0.918, RMSEE: 0.193) and verification set (R-Observed vs predicted(2) : 0.704, RMSEP: 0.289). The results obtained in this study showed that high CmbT affinities to ethidium, sulbactam, and sulfathiazole could be related to the absence of significant unfavourable interactions. In contrast, the presence of specific unfavourable interaction between two hydrogen bond donor groups in bacitracin, apramycin, novobiocin, vancomycin, kanamycin, gentamycin, and tobramycin is found to be the main reason for their lower CmbT affinities. In addition, membrane position of the CmbT binding site and positive correlation between substrates lipophilicity (log D-PH so) and CmbT affinity strongly indicates that CmbT recognizes its substrates within the membrane.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Journal of the Taiwan Institute of Chemical Engineers",
title = "Identifying the CmbT substrates specificity by using a quantitative structure-activity relationship (QSAR) study",
volume = "45",
number = "3",
pages = "764-771",
doi = "10.1016/j.jtice.2013.09.033"
}

Filipić, B., Nikolić, K., Filipić, S., Jovčić, B., Agbaba, D., Antić-Stanković, J., Kojić, M.,& Golić, N.. (2014). Identifying the CmbT substrates specificity by using a quantitative structure-activity relationship (QSAR) study. in Journal of the Taiwan Institute of Chemical Engineers
Elsevier Science BV, Amsterdam., 45(3), 764-771.
https://doi.org/10.1016/j.jtice.2013.09.033

Filipić B, Nikolić K, Filipić S, Jovčić B, Agbaba D, Antić-Stanković J, Kojić M, Golić N. Identifying the CmbT substrates specificity by using a quantitative structure-activity relationship (QSAR) study. in Journal of the Taiwan Institute of Chemical Engineers. 2014;45(3):764-771.
doi:10.1016/j.jtice.2013.09.033 .

Filipić, Brankica, Nikolić, Katarina, Filipić, Slavica, Jovčić, Branko, Agbaba, Danica, Antić-Stanković, Jelena, Kojić, Milan, Golić, Nataša, "Identifying the CmbT substrates specificity by using a quantitative structure-activity relationship (QSAR) study" in Journal of the Taiwan Institute of Chemical Engineers, 45, no. 3 (2014):764-771,
https://doi.org/10.1016/j.jtice.2013.09.033 . .

TY  - JOUR
AU  - Jovčić, Branko
AU  - Lepsanović, Zorica
AU  - Begović, Jelena
AU  - Filipić, Brankica
AU  - Kojić, Milan
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2173
AB  - New Delhi metallo-beta-lactamase producing Pseudomonas aeruginosa isolates are of special interest since P. aeruginosa is a major cause of nosocomial infections, the treatment of which could now be jeopardized, especially in developing countries. Six additional NDM-1 positive P. aeruginosa clinical isolates belonging to two different genotypes were shown to be plasmid-free. PFGE-hybridization experiments revealed the chromosomal location of the bla (NDM-1) gene. Restriction analysis and hybridization revealed that two copies of the bla (NDM-1) gene are present in the genomes of all tested isolates, as in previously characterized P. aeruginosa MMA83. Moreover, it was shown that increasing imipenem concentration did not have the effect on copy number of the bla (NDM-1) gene in the genome of P. aeruginosa MMA83.
PB  - Springer, Dordrecht
T2  - Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology
T1  - Two copies of bla (NDM-1) gene are present in NDM-1 producing Pseudomonas aeruginosa isolates from Serbia
VL  - 105
IS  - 3
SP  - 613
EP  - 618
DO  - 10.1007/s10482-013-0094-z
ER  - 

@article{
author = "Jovčić, Branko and Lepsanović, Zorica and Begović, Jelena and Filipić, Brankica and Kojić, Milan",
year = "2014",
abstract = "New Delhi metallo-beta-lactamase producing Pseudomonas aeruginosa isolates are of special interest since P. aeruginosa is a major cause of nosocomial infections, the treatment of which could now be jeopardized, especially in developing countries. Six additional NDM-1 positive P. aeruginosa clinical isolates belonging to two different genotypes were shown to be plasmid-free. PFGE-hybridization experiments revealed the chromosomal location of the bla (NDM-1) gene. Restriction analysis and hybridization revealed that two copies of the bla (NDM-1) gene are present in the genomes of all tested isolates, as in previously characterized P. aeruginosa MMA83. Moreover, it was shown that increasing imipenem concentration did not have the effect on copy number of the bla (NDM-1) gene in the genome of P. aeruginosa MMA83.",
publisher = "Springer, Dordrecht",
journal = "Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology",
title = "Two copies of bla (NDM-1) gene are present in NDM-1 producing Pseudomonas aeruginosa isolates from Serbia",
volume = "105",
number = "3",
pages = "613-618",
doi = "10.1007/s10482-013-0094-z"
}

Jovčić, B., Lepsanović, Z., Begović, J., Filipić, B.,& Kojić, M.. (2014). Two copies of bla (NDM-1) gene are present in NDM-1 producing Pseudomonas aeruginosa isolates from Serbia. in Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology
Springer, Dordrecht., 105(3), 613-618.
https://doi.org/10.1007/s10482-013-0094-z

Jovčić B, Lepsanović Z, Begović J, Filipić B, Kojić M. Two copies of bla (NDM-1) gene are present in NDM-1 producing Pseudomonas aeruginosa isolates from Serbia. in Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology. 2014;105(3):613-618.
doi:10.1007/s10482-013-0094-z .

Jovčić, Branko, Lepsanović, Zorica, Begović, Jelena, Filipić, Brankica, Kojić, Milan, "Two copies of bla (NDM-1) gene are present in NDM-1 producing Pseudomonas aeruginosa isolates from Serbia" in Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology, 105, no. 3 (2014):613-618,
https://doi.org/10.1007/s10482-013-0094-z . .

TY  - JOUR
AU  - Lukić, Jovanka
AU  - Strahinić, Ivana
AU  - Milenković, Marina
AU  - Nikolić, Milica
AU  - Tolinacki, Maja
AU  - Kojić, Milan
AU  - Begović, Jelena
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2070
AB  - Modern research in the area of probiotics is largely devoted to discovering factors that promote the adherence of probiotic candidates to host mucosal surfaces. The aim of the present study was to test the role of aggregation factor (AggL) and mucin-binding protein (MbpL) from Lactococcus sp. in adhesion to gastrointestinal mucosa. In vitro, ex vivo, and in vivo experiments in rats were used to assess the adhesive potential of these two proteins expressed in heterologous host Lactobacillus salivarius BGHO1. Although there was no influence of MbpL protein expression on BGHO1 adhesion to gut mucosa, expression of AggL had a negative effect on BGHO1 binding to ileal and colonic rat mucosa, as well as to human HT29-MTX cells and porcine gastric mucin in vitro. Because AggL did not decrease the adhesion of bacteria to intestinal fragments in ex vivo tests, where peristaltic simulation conditions were missing, we propose that intestinal motility could be a crucial force for eliminating aggregation-factor-bearing bacteria. Bacterial strains expressing aggregation factor could facilitate the removal of pathogens through the coaggregation mechanism, thus balancing gut microbial ecosystems in people affected by intestinal bacteria overgrowth.
PB  - Springer, New York
T2  - Microbial Ecology
T1  - Aggregation Factor as an Inhibitor of Bacterial Binding to Gut Mucosa
VL  - 68
IS  - 3
SP  - 633
EP  - 644
DO  - 10.1007/s00248-014-0426-1
ER  - 

@article{
author = "Lukić, Jovanka and Strahinić, Ivana and Milenković, Marina and Nikolić, Milica and Tolinacki, Maja and Kojić, Milan and Begović, Jelena",
year = "2014",
abstract = "Modern research in the area of probiotics is largely devoted to discovering factors that promote the adherence of probiotic candidates to host mucosal surfaces. The aim of the present study was to test the role of aggregation factor (AggL) and mucin-binding protein (MbpL) from Lactococcus sp. in adhesion to gastrointestinal mucosa. In vitro, ex vivo, and in vivo experiments in rats were used to assess the adhesive potential of these two proteins expressed in heterologous host Lactobacillus salivarius BGHO1. Although there was no influence of MbpL protein expression on BGHO1 adhesion to gut mucosa, expression of AggL had a negative effect on BGHO1 binding to ileal and colonic rat mucosa, as well as to human HT29-MTX cells and porcine gastric mucin in vitro. Because AggL did not decrease the adhesion of bacteria to intestinal fragments in ex vivo tests, where peristaltic simulation conditions were missing, we propose that intestinal motility could be a crucial force for eliminating aggregation-factor-bearing bacteria. Bacterial strains expressing aggregation factor could facilitate the removal of pathogens through the coaggregation mechanism, thus balancing gut microbial ecosystems in people affected by intestinal bacteria overgrowth.",
publisher = "Springer, New York",
journal = "Microbial Ecology",
title = "Aggregation Factor as an Inhibitor of Bacterial Binding to Gut Mucosa",
volume = "68",
number = "3",
pages = "633-644",
doi = "10.1007/s00248-014-0426-1"
}

Lukić, J., Strahinić, I., Milenković, M., Nikolić, M., Tolinacki, M., Kojić, M.,& Begović, J.. (2014). Aggregation Factor as an Inhibitor of Bacterial Binding to Gut Mucosa. in Microbial Ecology
Springer, New York., 68(3), 633-644.
https://doi.org/10.1007/s00248-014-0426-1

Lukić J, Strahinić I, Milenković M, Nikolić M, Tolinacki M, Kojić M, Begović J. Aggregation Factor as an Inhibitor of Bacterial Binding to Gut Mucosa. in Microbial Ecology. 2014;68(3):633-644.
doi:10.1007/s00248-014-0426-1 .

Lukić, Jovanka, Strahinić, Ivana, Milenković, Marina, Nikolić, Milica, Tolinacki, Maja, Kojić, Milan, Begović, Jelena, "Aggregation Factor as an Inhibitor of Bacterial Binding to Gut Mucosa" in Microbial Ecology, 68, no. 3 (2014):633-644,
https://doi.org/10.1007/s00248-014-0426-1 . .

TY  - THES
AU  - Filipić, Brankica
PY  - 2013
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=1009
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:7635/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=44873999
UR  - http://nardus.mpn.gov.rs/123456789/3155
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3387
AB  - Lactococcus lactis pripada grupi bakterija mlečne kiseline (BMK) i imadugotrajnu primenu u prehrambenoj industriji kao starter kultura, najviše u proizvodnjitvrdih i polutvrdih sireva. Kako L. lactis ima ključnu ulogu u formiranju ukusa iteksture finalnog proizvoda, a doprinosi i produženju trajnosti namirnica, velikiekonomski i industrijski značaj doveo je do toga da su laktokoke predmet stalnogizučavanja. Iako se generalno smatraju bezbednim mikroorganizmima, mnogiistraživači ukazuju na činjenicu da laktokoke, kao i druge bakterije mlečne kiselinemogu biti rezervoar gena za rezistenciju. U cilju sprečavanja širenja ovakvih gena nadruge bakterije intestinalnog trakta ili patogene bakterije prisutne u hrani, od velikogznačaja je izučavanje mehanizama rezistencije, posebno kod laktokoka namenjenih zaprehrambenu industriju.Analizom genoma L. lactis detektovano je prisustvo 40 potencijalnih gena zaMDR (eng. multidrug resistance) transportere, od čega su svega dva ABC transportera,LmrA i LmrCD i jedan MFS transporter, LmrP funkcionalno okarakterisani.Istraživanja prikazana u ovom radu su imala za cilj da se okarakteriše CmbT(eng. Cysteine and Methionine Biosynthesis Transporter) protein, potencijalno noviMDR transporter u soju L. lactis.Gen cmbT identifikovan je po prvi put prilikom izučavanja regulacijemetabolizma sumpora u L. lactis. Primenom različitih kompjuterskih programa,pokazano je da cmbT kodira transmembranski integralni efluks protein (veličine 454aminokiseline) koji pokazuje homologiju sa članovima 2.A.1.3.X supstrat/H+ antiporter-2 subfamilije.Prvi korak u funkcionalnoj karakterizaciji CmbT proteina bio je kloniranje iekspresija cmbT gena pomoću precizno regulisanog NICE (eng. NIsin Controlled gene Expression) sistema...
AB  - Lactococcus lactis is a lactic acid bacterium (LAB) widely used as a constituentof many industrial and artisanal starter cultures in dairy industry, especially forfermentation of hard and semi hard cheeses. This organism plays a key role in theformation of flavour and texture of cheese and its preservation and because of the greatindustrial importance it has been the subject of numerous studies. Although they haveacquired the „Generaly Regarded As Safe“ (GRAS) status, many investigators havespeculated that lactococci as well as other LAB may act as reservoirs of antibioticresistance genes. The main threat associated with these bacteria is that they can transferresistance genes to intestinal microorganisms and food-associated pathogenic bacteria.In order to prevent and reduce the spreading of the resistance genes, more studies ofmultidrug resistance (MDR) transporters in lactococci intended for use in food systemsare needed.The genome analysis of L. lactis indicated the presence of at least 40 putativedrug transporter genes, but only two ABC transporters, LmrA, and LmrCD, and oneMFS transporter, LmrP, have been studied in detail and all three have demonstratedMDR activity experimentally.The aim of this thesis was to investigate and characterize new MDR transporterCmbT (Cysteine and Methionine Biosynthesis Transporter) in L. lactis.The cmbT gene was originally identified from a random mutagenesis study ofcysteine and methionine biosynthesis regulation in L. lactis. This gene is predicted toencode an integral membrane efflux protein (454 amino acids) with homology tomembers of the 2.A.1.3.X drug/H+ antiporter-2 subfamily of the major facilitatorsuperfamily (MFS).In order to follow the transport function of the CmbT protein, the cmbT genewas cloned and over-expressed in L. lactis NZ9000 using the nisin controlled gene expression (NICE) system...
PB  - Универзитет у Београду, Фармацеутски факултет
T2  - Универзитет у Београду
T1  - Funkcionalna karakterizacija CmbT transportera odgovornog za višestruke rezistencije na strukturno različite supstrate u soju Lactococcus lactis subsp. cremoris MG1363
T1  - Functional characterization of the CmbT transporter responsible for multidrug resistance on structurally different substrates of the strain Lactococcus lactis subsp. cremoris MG1363
UR  - https://hdl.handle.net/21.15107/rcub_nardus_3155
ER  - 

@phdthesis{
author = "Filipić, Brankica",
year = "2013",
abstract = "Lactococcus lactis pripada grupi bakterija mlečne kiseline (BMK) i imadugotrajnu primenu u prehrambenoj industriji kao starter kultura, najviše u proizvodnjitvrdih i polutvrdih sireva. Kako L. lactis ima ključnu ulogu u formiranju ukusa iteksture finalnog proizvoda, a doprinosi i produženju trajnosti namirnica, velikiekonomski i industrijski značaj doveo je do toga da su laktokoke predmet stalnogizučavanja. Iako se generalno smatraju bezbednim mikroorganizmima, mnogiistraživači ukazuju na činjenicu da laktokoke, kao i druge bakterije mlečne kiselinemogu biti rezervoar gena za rezistenciju. U cilju sprečavanja širenja ovakvih gena nadruge bakterije intestinalnog trakta ili patogene bakterije prisutne u hrani, od velikogznačaja je izučavanje mehanizama rezistencije, posebno kod laktokoka namenjenih zaprehrambenu industriju.Analizom genoma L. lactis detektovano je prisustvo 40 potencijalnih gena zaMDR (eng. multidrug resistance) transportere, od čega su svega dva ABC transportera,LmrA i LmrCD i jedan MFS transporter, LmrP funkcionalno okarakterisani.Istraživanja prikazana u ovom radu su imala za cilj da se okarakteriše CmbT(eng. Cysteine and Methionine Biosynthesis Transporter) protein, potencijalno noviMDR transporter u soju L. lactis.Gen cmbT identifikovan je po prvi put prilikom izučavanja regulacijemetabolizma sumpora u L. lactis. Primenom različitih kompjuterskih programa,pokazano je da cmbT kodira transmembranski integralni efluks protein (veličine 454aminokiseline) koji pokazuje homologiju sa članovima 2.A.1.3.X supstrat/H+ antiporter-2 subfamilije.Prvi korak u funkcionalnoj karakterizaciji CmbT proteina bio je kloniranje iekspresija cmbT gena pomoću precizno regulisanog NICE (eng. NIsin Controlled gene Expression) sistema..., Lactococcus lactis is a lactic acid bacterium (LAB) widely used as a constituentof many industrial and artisanal starter cultures in dairy industry, especially forfermentation of hard and semi hard cheeses. This organism plays a key role in theformation of flavour and texture of cheese and its preservation and because of the greatindustrial importance it has been the subject of numerous studies. Although they haveacquired the „Generaly Regarded As Safe“ (GRAS) status, many investigators havespeculated that lactococci as well as other LAB may act as reservoirs of antibioticresistance genes. The main threat associated with these bacteria is that they can transferresistance genes to intestinal microorganisms and food-associated pathogenic bacteria.In order to prevent and reduce the spreading of the resistance genes, more studies ofmultidrug resistance (MDR) transporters in lactococci intended for use in food systemsare needed.The genome analysis of L. lactis indicated the presence of at least 40 putativedrug transporter genes, but only two ABC transporters, LmrA, and LmrCD, and oneMFS transporter, LmrP, have been studied in detail and all three have demonstratedMDR activity experimentally.The aim of this thesis was to investigate and characterize new MDR transporterCmbT (Cysteine and Methionine Biosynthesis Transporter) in L. lactis.The cmbT gene was originally identified from a random mutagenesis study ofcysteine and methionine biosynthesis regulation in L. lactis. This gene is predicted toencode an integral membrane efflux protein (454 amino acids) with homology tomembers of the 2.A.1.3.X drug/H+ antiporter-2 subfamily of the major facilitatorsuperfamily (MFS).In order to follow the transport function of the CmbT protein, the cmbT genewas cloned and over-expressed in L. lactis NZ9000 using the nisin controlled gene expression (NICE) system...",
publisher = "Универзитет у Београду, Фармацеутски факултет",
journal = "Универзитет у Београду",
title = "Funkcionalna karakterizacija CmbT transportera odgovornog za višestruke rezistencije na strukturno različite supstrate u soju Lactococcus lactis subsp. cremoris MG1363, Functional characterization of the CmbT transporter responsible for multidrug resistance on structurally different substrates of the strain Lactococcus lactis subsp. cremoris MG1363",
url = "https://hdl.handle.net/21.15107/rcub_nardus_3155"
}

Filipić, B.. (2013). Funkcionalna karakterizacija CmbT transportera odgovornog za višestruke rezistencije na strukturno različite supstrate u soju Lactococcus lactis subsp. cremoris MG1363. in Универзитет у Београду
Универзитет у Београду, Фармацеутски факултет..
https://hdl.handle.net/21.15107/rcub_nardus_3155

Filipić B. Funkcionalna karakterizacija CmbT transportera odgovornog za višestruke rezistencije na strukturno različite supstrate u soju Lactococcus lactis subsp. cremoris MG1363. in Универзитет у Београду. 2013;.
https://hdl.handle.net/21.15107/rcub_nardus_3155 .

Filipić, Brankica, "Funkcionalna karakterizacija CmbT transportera odgovornog za višestruke rezistencije na strukturno različite supstrate u soju Lactococcus lactis subsp. cremoris MG1363" in Универзитет у Београду (2013),
https://hdl.handle.net/21.15107/rcub_nardus_3155 .