TY  - JOUR
AU  - Milić, Mirta
AU  - Ceppi, Marcello
AU  - Bruzzone, Marco
AU  - Azqueta, Amaya
AU  - Brunborg, Gunnar
AU  - Godschalk, Roger
AU  - Koppen, Gudrun
AU  - Langie, Sabine
AU  - Møller, Peter
AU  - Teixeira, João Paulo
AU  - Alija, Avdulla
AU  - Anderson, Diana
AU  - Andrade, Vanessa
AU  - Andreoli, Cristina
AU  - Asllani, Fisnik
AU  - Bangkoglu, Ezgi Eyluel
AU  - Barančoková, Magdalena
AU  - Basaran, Nursen
AU  - Boutet-Robinet, Elisa
AU  - Buschini, Annamaria
AU  - Cavallo, Delia
AU  - Costa Pereira, Cristiana
AU  - Costa, Carla
AU  - Costa, Solange
AU  - Da Silva, Juliana
AU  - Del Boˊ, Cristian
AU  - Dimitrijević Srećković, Vesna
AU  - Đelić, Ninoslav
AU  - Dobrzyńska, Malgorzata
AU  - Duračková, Zdenka
AU  - Dvořáková, Monika
AU  - Gajski, Goran
AU  - Galati, Serena
AU  - García Lima, Omar
AU  - Giovannelli, Lisa
AU  - Goroshinskaya, Irina A.
AU  - Grindel, Annemarie
AU  - Gutzkow, Kristine B.
AU  - Hernández, Alba
AU  - Hernández, Carlos
AU  - Holven, Kirsten B.
AU  - Ibero-Baraibar, Idoia
AU  - Ottestad, Inger
AU  - Kadioglu, Ela
AU  - Kažimirová, Alena
AU  - Kuznetsova, Elena
AU  - Ladeira, Carina
AU  - Laffon, Blanca
AU  - Lamonaca, Palma
AU  - Lebailly, Pierre
AU  - Louro, Henriqueta
AU  - Mandina Cardoso, Tania
AU  - Marcon, Francesca
AU  - Marcos, Ricard
AU  - Moretti, Massimo
AU  - Moretti, Silvia
AU  - Najafzadeh, Mojgan
AU  - Nemeth, Zsuzsanna
AU  - Neri, Monica
AU  - Novotna, Bozena
AU  - Orlow, Irene
AU  - Paduchova, Zuzana
AU  - Pastor, Susana
AU  - Perdry, Hervé
AU  - Spremo-Potparević, Biljana
AU  - Ramadhani, Dwi
AU  - Riso, Patrizia
AU  - Rohr, Paula
AU  - Rojas, Emilio
AU  - Rossner, Pavel
AU  - Safar, Anna
AU  - Sardas, Semra
AU  - Silva, Maria João
AU  - Sirota, Nikolay
AU  - Smolkova, Bozena
AU  - Staruchova, Marta
AU  - Stetina, Rudolf
AU  - Stopper, Helga
AU  - Surikova, Ekaterina I.
AU  - Ulven, Stine M.
AU  - Ursini, Cinzia Lucia
AU  - Valdiglesias, Vanessa
AU  - Valverde, Mahara
AU  - Vodicka, Pavel
AU  - Volkovova, Katarina
AU  - Wagner, Karl-Heinz
AU  - Živković, Lada
AU  - Dušinská, Maria
AU  - Collins, Andrew R.
AU  - Bonassi, Stefano
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3791
AB  - The alkaline comet assay, or single cell gel electrophoresis, is one of the most popular methods for assessing DNA damage in human population. One of the open issues concerning this assay is the identification of those factors that can explain the large inter-individual and inter-laboratory variation. International collaborative initiatives such as the hCOMET project - a COST Action launched in 2016 - represent a valuable tool to meet this challenge. The aims of hCOMET were to establish reference values for the level of DNA damage in humans, to investigate the effect of host factors, lifestyle and exposure to genotoxic agents, and to compare different sources of assay variability. A database of 19,320 subjects was generated, pooling data from 105 studies run by 44 laboratories in 26 countries between 1999 and 2019. A mixed random effect log-linear model, in parallel with a classic meta-analysis, was applied to take into account the extensive heterogeneity of data, due to descriptor, specimen and protocol variability. As a result of this analysis interquartile intervals of DNA strand breaks (which includes alkali-labile sites) were reported for tail intensity, tail length, and tail moment (comet assay descriptors). A small variation by age was reported in some datasets, suggesting higher DNA damage in oldest age-classes, while no effect could be shown for sex or smoking habit, although the lack of data on heavy smokers has still to be considered. Finally, highly significant differences in DNA damage were found for most exposures investigated in specific studies. In conclusion, these data, which confirm that DNA damage measured by the comet assay is an excellent biomarker of exposure in several conditions, may contribute to improving the quality of study design and to the standardization of results of the comet assay in human populations.
PB  - Elsevier B.V.
T2  - Mutation Research - Reviews in Mutation Research
T1  - The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders
VL  - 787
DO  - 10.1016/j.mrrev.2021.108371
ER  - 

@article{
author = "Milić, Mirta and Ceppi, Marcello and Bruzzone, Marco and Azqueta, Amaya and Brunborg, Gunnar and Godschalk, Roger and Koppen, Gudrun and Langie, Sabine and Møller, Peter and Teixeira, João Paulo and Alija, Avdulla and Anderson, Diana and Andrade, Vanessa and Andreoli, Cristina and Asllani, Fisnik and Bangkoglu, Ezgi Eyluel and Barančoková, Magdalena and Basaran, Nursen and Boutet-Robinet, Elisa and Buschini, Annamaria and Cavallo, Delia and Costa Pereira, Cristiana and Costa, Carla and Costa, Solange and Da Silva, Juliana and Del Boˊ, Cristian and Dimitrijević Srećković, Vesna and Đelić, Ninoslav and Dobrzyńska, Malgorzata and Duračková, Zdenka and Dvořáková, Monika and Gajski, Goran and Galati, Serena and García Lima, Omar and Giovannelli, Lisa and Goroshinskaya, Irina A. and Grindel, Annemarie and Gutzkow, Kristine B. and Hernández, Alba and Hernández, Carlos and Holven, Kirsten B. and Ibero-Baraibar, Idoia and Ottestad, Inger and Kadioglu, Ela and Kažimirová, Alena and Kuznetsova, Elena and Ladeira, Carina and Laffon, Blanca and Lamonaca, Palma and Lebailly, Pierre and Louro, Henriqueta and Mandina Cardoso, Tania and Marcon, Francesca and Marcos, Ricard and Moretti, Massimo and Moretti, Silvia and Najafzadeh, Mojgan and Nemeth, Zsuzsanna and Neri, Monica and Novotna, Bozena and Orlow, Irene and Paduchova, Zuzana and Pastor, Susana and Perdry, Hervé and Spremo-Potparević, Biljana and Ramadhani, Dwi and Riso, Patrizia and Rohr, Paula and Rojas, Emilio and Rossner, Pavel and Safar, Anna and Sardas, Semra and Silva, Maria João and Sirota, Nikolay and Smolkova, Bozena and Staruchova, Marta and Stetina, Rudolf and Stopper, Helga and Surikova, Ekaterina I. and Ulven, Stine M. and Ursini, Cinzia Lucia and Valdiglesias, Vanessa and Valverde, Mahara and Vodicka, Pavel and Volkovova, Katarina and Wagner, Karl-Heinz and Živković, Lada and Dušinská, Maria and Collins, Andrew R. and Bonassi, Stefano",
year = "2021",
abstract = "The alkaline comet assay, or single cell gel electrophoresis, is one of the most popular methods for assessing DNA damage in human population. One of the open issues concerning this assay is the identification of those factors that can explain the large inter-individual and inter-laboratory variation. International collaborative initiatives such as the hCOMET project - a COST Action launched in 2016 - represent a valuable tool to meet this challenge. The aims of hCOMET were to establish reference values for the level of DNA damage in humans, to investigate the effect of host factors, lifestyle and exposure to genotoxic agents, and to compare different sources of assay variability. A database of 19,320 subjects was generated, pooling data from 105 studies run by 44 laboratories in 26 countries between 1999 and 2019. A mixed random effect log-linear model, in parallel with a classic meta-analysis, was applied to take into account the extensive heterogeneity of data, due to descriptor, specimen and protocol variability. As a result of this analysis interquartile intervals of DNA strand breaks (which includes alkali-labile sites) were reported for tail intensity, tail length, and tail moment (comet assay descriptors). A small variation by age was reported in some datasets, suggesting higher DNA damage in oldest age-classes, while no effect could be shown for sex or smoking habit, although the lack of data on heavy smokers has still to be considered. Finally, highly significant differences in DNA damage were found for most exposures investigated in specific studies. In conclusion, these data, which confirm that DNA damage measured by the comet assay is an excellent biomarker of exposure in several conditions, may contribute to improving the quality of study design and to the standardization of results of the comet assay in human populations.",
publisher = "Elsevier B.V.",
journal = "Mutation Research - Reviews in Mutation Research",
title = "The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders",
volume = "787",
doi = "10.1016/j.mrrev.2021.108371"
}

Milić, M., Ceppi, M., Bruzzone, M., Azqueta, A., Brunborg, G., Godschalk, R., Koppen, G., Langie, S., Møller, P., Teixeira, J. P., Alija, A., Anderson, D., Andrade, V., Andreoli, C., Asllani, F., Bangkoglu, E. E., Barančoková, M., Basaran, N., Boutet-Robinet, E., Buschini, A., Cavallo, D., Costa Pereira, C., Costa, C., Costa, S., Da Silva, J., Del Boˊ, C., Dimitrijević Srećković, V., Đelić, N., Dobrzyńska, M., Duračková, Z., Dvořáková, M., Gajski, G., Galati, S., García Lima, O., Giovannelli, L., Goroshinskaya, I. A., Grindel, A., Gutzkow, K. B., Hernández, A., Hernández, C., Holven, K. B., Ibero-Baraibar, I., Ottestad, I., Kadioglu, E., Kažimirová, A., Kuznetsova, E., Ladeira, C., Laffon, B., Lamonaca, P., Lebailly, P., Louro, H., Mandina Cardoso, T., Marcon, F., Marcos, R., Moretti, M., Moretti, S., Najafzadeh, M., Nemeth, Z., Neri, M., Novotna, B., Orlow, I., Paduchova, Z., Pastor, S., Perdry, H., Spremo-Potparević, B., Ramadhani, D., Riso, P., Rohr, P., Rojas, E., Rossner, P., Safar, A., Sardas, S., Silva, M. J., Sirota, N., Smolkova, B., Staruchova, M., Stetina, R., Stopper, H., Surikova, E. I., Ulven, S. M., Ursini, C. L., Valdiglesias, V., Valverde, M., Vodicka, P., Volkovova, K., Wagner, K., Živković, L., Dušinská, M., Collins, A. R.,& Bonassi, S.. (2021). The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders. in Mutation Research - Reviews in Mutation Research
Elsevier B.V.., 787.
https://doi.org/10.1016/j.mrrev.2021.108371

Milić M, Ceppi M, Bruzzone M, Azqueta A, Brunborg G, Godschalk R, Koppen G, Langie S, Møller P, Teixeira JP, Alija A, Anderson D, Andrade V, Andreoli C, Asllani F, Bangkoglu EE, Barančoková M, Basaran N, Boutet-Robinet E, Buschini A, Cavallo D, Costa Pereira C, Costa C, Costa S, Da Silva J, Del Boˊ C, Dimitrijević Srećković V, Đelić N, Dobrzyńska M, Duračková Z, Dvořáková M, Gajski G, Galati S, García Lima O, Giovannelli L, Goroshinskaya IA, Grindel A, Gutzkow KB, Hernández A, Hernández C, Holven KB, Ibero-Baraibar I, Ottestad I, Kadioglu E, Kažimirová A, Kuznetsova E, Ladeira C, Laffon B, Lamonaca P, Lebailly P, Louro H, Mandina Cardoso T, Marcon F, Marcos R, Moretti M, Moretti S, Najafzadeh M, Nemeth Z, Neri M, Novotna B, Orlow I, Paduchova Z, Pastor S, Perdry H, Spremo-Potparević B, Ramadhani D, Riso P, Rohr P, Rojas E, Rossner P, Safar A, Sardas S, Silva MJ, Sirota N, Smolkova B, Staruchova M, Stetina R, Stopper H, Surikova EI, Ulven SM, Ursini CL, Valdiglesias V, Valverde M, Vodicka P, Volkovova K, Wagner K, Živković L, Dušinská M, Collins AR, Bonassi S. The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders. in Mutation Research - Reviews in Mutation Research. 2021;787.
doi:10.1016/j.mrrev.2021.108371 .

Milić, Mirta, Ceppi, Marcello, Bruzzone, Marco, Azqueta, Amaya, Brunborg, Gunnar, Godschalk, Roger, Koppen, Gudrun, Langie, Sabine, Møller, Peter, Teixeira, João Paulo, Alija, Avdulla, Anderson, Diana, Andrade, Vanessa, Andreoli, Cristina, Asllani, Fisnik, Bangkoglu, Ezgi Eyluel, Barančoková, Magdalena, Basaran, Nursen, Boutet-Robinet, Elisa, Buschini, Annamaria, Cavallo, Delia, Costa Pereira, Cristiana, Costa, Carla, Costa, Solange, Da Silva, Juliana, Del Boˊ, Cristian, Dimitrijević Srećković, Vesna, Đelić, Ninoslav, Dobrzyńska, Malgorzata, Duračková, Zdenka, Dvořáková, Monika, Gajski, Goran, Galati, Serena, García Lima, Omar, Giovannelli, Lisa, Goroshinskaya, Irina A., Grindel, Annemarie, Gutzkow, Kristine B., Hernández, Alba, Hernández, Carlos, Holven, Kirsten B., Ibero-Baraibar, Idoia, Ottestad, Inger, Kadioglu, Ela, Kažimirová, Alena, Kuznetsova, Elena, Ladeira, Carina, Laffon, Blanca, Lamonaca, Palma, Lebailly, Pierre, Louro, Henriqueta, Mandina Cardoso, Tania, Marcon, Francesca, Marcos, Ricard, Moretti, Massimo, Moretti, Silvia, Najafzadeh, Mojgan, Nemeth, Zsuzsanna, Neri, Monica, Novotna, Bozena, Orlow, Irene, Paduchova, Zuzana, Pastor, Susana, Perdry, Hervé, Spremo-Potparević, Biljana, Ramadhani, Dwi, Riso, Patrizia, Rohr, Paula, Rojas, Emilio, Rossner, Pavel, Safar, Anna, Sardas, Semra, Silva, Maria João, Sirota, Nikolay, Smolkova, Bozena, Staruchova, Marta, Stetina, Rudolf, Stopper, Helga, Surikova, Ekaterina I., Ulven, Stine M., Ursini, Cinzia Lucia, Valdiglesias, Vanessa, Valverde, Mahara, Vodicka, Pavel, Volkovova, Katarina, Wagner, Karl-Heinz, Živković, Lada, Dušinská, Maria, Collins, Andrew R., Bonassi, Stefano, "The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders" in Mutation Research - Reviews in Mutation Research, 787 (2021),
https://doi.org/10.1016/j.mrrev.2021.108371 . .

TY  - JOUR
AU  - Bajić, Vladan. P.
AU  - Essack, Magbubah
AU  - Živković, Lada
AU  - Stewart, Alan
AU  - Zafirović, Sonja
AU  - Bajić, Vladimir B.
AU  - Gojobori, Takashi
AU  - Isenović, Esma
AU  - Spremo-Potparević, Biljana
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3554
AB  - Alzheimer’s disease (AD) is a neurodegenerative disease that affects millions of individuals worldwide and can occur relatively early or later in life. It is well known that genetic components, such as the amyloid precursor protein gene on chromosome 21, are fundamental in early-onset AD (EOAD). To date, however, only the apolipoprotein E4 (ApoE4) gene has been proved to be a genetic risk factor for late-onset AD (LOAD). In recent years, despite the hypothesis that many additional unidentified genes are likely to play a role in AD development, it is surprising that additional gene polymorphisms associated with LOAD have failed to come to light. In this review, we examine the role of X chromosome epigenetics and, based upon GWAS studies, the PCDHX11 gene. Furthermore, we explore other genetic risk factors of AD that involve X-chromosome epigenetics.
PB  - Frontiers Media S.A.
T2  - Frontiers in Genetics
T1  - The X Files: “The Mystery of X Chromosome Instability in Alzheimer’s Disease”
VL  - 10
DO  - 10.3389/fgene.2019.01368
ER  - 

@article{
author = "Bajić, Vladan. P. and Essack, Magbubah and Živković, Lada and Stewart, Alan and Zafirović, Sonja and Bajić, Vladimir B. and Gojobori, Takashi and Isenović, Esma and Spremo-Potparević, Biljana",
year = "2020",
abstract = "Alzheimer’s disease (AD) is a neurodegenerative disease that affects millions of individuals worldwide and can occur relatively early or later in life. It is well known that genetic components, such as the amyloid precursor protein gene on chromosome 21, are fundamental in early-onset AD (EOAD). To date, however, only the apolipoprotein E4 (ApoE4) gene has been proved to be a genetic risk factor for late-onset AD (LOAD). In recent years, despite the hypothesis that many additional unidentified genes are likely to play a role in AD development, it is surprising that additional gene polymorphisms associated with LOAD have failed to come to light. In this review, we examine the role of X chromosome epigenetics and, based upon GWAS studies, the PCDHX11 gene. Furthermore, we explore other genetic risk factors of AD that involve X-chromosome epigenetics.",
publisher = "Frontiers Media S.A.",
journal = "Frontiers in Genetics",
title = "The X Files: “The Mystery of X Chromosome Instability in Alzheimer’s Disease”",
volume = "10",
doi = "10.3389/fgene.2019.01368"
}

Bajić, Vladan. P., Essack, M., Živković, L., Stewart, A., Zafirović, S., Bajić, V. B., Gojobori, T., Isenović, E.,& Spremo-Potparević, B.. (2020). The X Files: “The Mystery of X Chromosome Instability in Alzheimer’s Disease”. in Frontiers in Genetics
Frontiers Media S.A.., 10.
https://doi.org/10.3389/fgene.2019.01368

Bajić VP, Essack M, Živković L, Stewart A, Zafirović S, Bajić VB, Gojobori T, Isenović E, Spremo-Potparević B. The X Files: “The Mystery of X Chromosome Instability in Alzheimer’s Disease”. in Frontiers in Genetics. 2020;10.
doi:10.3389/fgene.2019.01368 .

Bajić, Vladan. P., Essack, Magbubah, Živković, Lada, Stewart, Alan, Zafirović, Sonja, Bajić, Vladimir B., Gojobori, Takashi, Isenović, Esma, Spremo-Potparević, Biljana, "The X Files: “The Mystery of X Chromosome Instability in Alzheimer’s Disease”" in Frontiers in Genetics, 10 (2020),
https://doi.org/10.3389/fgene.2019.01368 . .

TY  - JOUR
AU  - Obradović, Milan
AU  - Zafirović, Sonja
AU  - Essack, Magbubah
AU  - Dimitrov, Jelena
AU  - Živković, Lada
AU  - Spremo-Potparević, Biljana
AU  - Radak, Đorđe
AU  - Bajić, Vladimir B.
AU  - Isenović, Esma
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3462
AB  - To remedy carotid artery stenosis and prevent stroke surgical intervention is commonly used, and the gold standard being carotid endarterectomy (CEA). During CEA cerebrovascular hemoglobin oxygen saturation decreases and when this decrease reaches critical levels it leads to cerebral hypoxia that causes neuronal damage. One of the proposed mechanism that affects changes during CEA and contribute to acute brain ischemia (ABI) is oxidative stress. The increased production of reactive oxygen species and reactive nitrogen species during ABI may cause an unregulated inflammatory response and further lead to structural and functional injury of neurons. Antioxidant activity are involved in the protection against neuronal damage after cerebral ischemia. We hypothesized that neuronal injury and poor outcomes in patients undergoing CEA may be results of oxidative stress that disturbed function of antioxidant enzymes and contributed to the DNA damage in lymphocytes.
PB  - Elsevier
T2  - Medical Hypotheses
T1  - Antioxidant enzymes expression in lymphocytes of patients undergoing carotid endarterectomy
VL  - 134
SP  - 1
EP  - 5
DO  - 10.1016/j.mehy.2019.109419
ER  - 

@article{
author = "Obradović, Milan and Zafirović, Sonja and Essack, Magbubah and Dimitrov, Jelena and Živković, Lada and Spremo-Potparević, Biljana and Radak, Đorđe and Bajić, Vladimir B. and Isenović, Esma",
year = "2020",
abstract = "To remedy carotid artery stenosis and prevent stroke surgical intervention is commonly used, and the gold standard being carotid endarterectomy (CEA). During CEA cerebrovascular hemoglobin oxygen saturation decreases and when this decrease reaches critical levels it leads to cerebral hypoxia that causes neuronal damage. One of the proposed mechanism that affects changes during CEA and contribute to acute brain ischemia (ABI) is oxidative stress. The increased production of reactive oxygen species and reactive nitrogen species during ABI may cause an unregulated inflammatory response and further lead to structural and functional injury of neurons. Antioxidant activity are involved in the protection against neuronal damage after cerebral ischemia. We hypothesized that neuronal injury and poor outcomes in patients undergoing CEA may be results of oxidative stress that disturbed function of antioxidant enzymes and contributed to the DNA damage in lymphocytes.",
publisher = "Elsevier",
journal = "Medical Hypotheses",
title = "Antioxidant enzymes expression in lymphocytes of patients undergoing carotid endarterectomy",
volume = "134",
pages = "1-5",
doi = "10.1016/j.mehy.2019.109419"
}

Obradović, M., Zafirović, S., Essack, M., Dimitrov, J., Živković, L., Spremo-Potparević, B., Radak, Đ., Bajić, V. B.,& Isenović, E.. (2020). Antioxidant enzymes expression in lymphocytes of patients undergoing carotid endarterectomy. in Medical Hypotheses
Elsevier., 134, 1-5.
https://doi.org/10.1016/j.mehy.2019.109419

Obradović M, Zafirović S, Essack M, Dimitrov J, Živković L, Spremo-Potparević B, Radak Đ, Bajić VB, Isenović E. Antioxidant enzymes expression in lymphocytes of patients undergoing carotid endarterectomy. in Medical Hypotheses. 2020;134:1-5.
doi:10.1016/j.mehy.2019.109419 .

Obradović, Milan, Zafirović, Sonja, Essack, Magbubah, Dimitrov, Jelena, Živković, Lada, Spremo-Potparević, Biljana, Radak, Đorđe, Bajić, Vladimir B., Isenović, Esma, "Antioxidant enzymes expression in lymphocytes of patients undergoing carotid endarterectomy" in Medical Hypotheses, 134 (2020):1-5,
https://doi.org/10.1016/j.mehy.2019.109419 . .

TY  - JOUR
AU  - Živković, Lada
AU  - Borozan, Sunčica
AU  - Bajić, Vladan
AU  - Đorđević, Stefana
AU  - Hristov, Aleksandar
AU  - Spremo-Potparević, Biljana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3595
AB  - Objective. Prooxidants and antioxidants affect the oxidative balance at the intracellular level. Oxidative stress is a consequence of the overproduction of prooxidants and is caused by disturbances in the balance of oxidative reduction processes. Non-enzymatic low molecular weight antioxidants can be introduced into the body through food. Cordyceps sinensis (C. sinensis) is a medicinal fungus used in traditional Chinese medicine, with rich content of vitamins, various polysaccharides, and many nucleosides. The aim of this study is to evaluate the antioxidant capacity of the dietary supplement C. sinensis. Methods. The capacity of the hydroxyl radical scavenger activity, the total antioxidant activity of FRAP (Ferric Reducing Antioxidant Power) and the DPPH (2,2-diphenyl-1- picrylhydrazyl) scavenger activity were measured. Results. C. sinensis at the tested concentrations of 0.0078–2.00 mg/mL had a pronounced ability to remove hydroxyl radicals with IC50 of 0.5 mg/mL, while at concentrations (0.0078–10.00 mg / mL) it showed a moderate reducing ability. C sinensis showed no ability to remove DPPH radicals. Conclusion. C. sinensis effectively removes hydroxyl radicals, for which the body does not have adequate antioxidant protection, so we can include it in the group of free radical scavengers.
AB  - Cilj. Prooksidansi i antioksidansi utiču na oksidativnu ravnotežu na intracelularnom nivou. Oksidativni stres je posledica prekomerne produkcije prooksidanasa i nastaje usled poremećaja u ravnoteži oksido-redukcionih procesa. Neenzimski antioksidansi male molekulske mase mogu se uneti u organizam preko hrane. Cordyceps sinensis (C. sinensis) lekovita je gljiva koja se koristi u tradicionalnoj kineskoj medicini, ima bogat sadržaj vitamina, raznih polisaharida, kao i mnogih nukleozida. Cilj istraživanja ove studije bila je evaluacija antioksidativnog kapaciteta dijetetskog suplementa C. sinensis. Metode. Mereni su kapacitet „skevindžer“ aktivnosti hidroksil radikala, ukupna antioksidativna aktivnost primenom FRAP (Ferric Reducing Antioxidant Power) metode i DPPH (2,2-difenil-1-pikrilhidrazil) – skevindžer aktivnost. Rezultati. C. sinensis je u ispitivanim koncentracijama 0,0078–2,00 mg/mL imao izraženu sposobnost uklanjanja hidroskil radikala, čija je IC50 iznosila 0,5 mg/mL, dok je u koncentracijama 0,0078–10,00 mg/mL pokazao umerenu redukcionu sposobnost. C. sinensis nije pokazao sposobnost uklanjanja DPPH radikala. Zaključak. C. sinensis efikasno neutrališe hidroksilne radikale, za koje organizam nema adekvatnu antioksidativnu zaštitu pa ga možemo uvrstiti u grupu potencijalnih protektora od slobodnih radikala.
PB  - Serbian Medical Society
T2  - Medicinski Casopis
T1  - Evaluation of antioxidant potential of cordyceps sinensis in vitro
T1  - Evaluacija antioksidativnog potencijala gljive Cordyceps sinensis in vitro
VL  - 53
IS  - 4
SP  - 129
EP  - 134
DO  - 10.5937/mckg53-24450
ER  - 

@article{
author = "Živković, Lada and Borozan, Sunčica and Bajić, Vladan and Đorđević, Stefana and Hristov, Aleksandar and Spremo-Potparević, Biljana",
year = "2019",
abstract = "Objective. Prooxidants and antioxidants affect the oxidative balance at the intracellular level. Oxidative stress is a consequence of the overproduction of prooxidants and is caused by disturbances in the balance of oxidative reduction processes. Non-enzymatic low molecular weight antioxidants can be introduced into the body through food. Cordyceps sinensis (C. sinensis) is a medicinal fungus used in traditional Chinese medicine, with rich content of vitamins, various polysaccharides, and many nucleosides. The aim of this study is to evaluate the antioxidant capacity of the dietary supplement C. sinensis. Methods. The capacity of the hydroxyl radical scavenger activity, the total antioxidant activity of FRAP (Ferric Reducing Antioxidant Power) and the DPPH (2,2-diphenyl-1- picrylhydrazyl) scavenger activity were measured. Results. C. sinensis at the tested concentrations of 0.0078–2.00 mg/mL had a pronounced ability to remove hydroxyl radicals with IC50 of 0.5 mg/mL, while at concentrations (0.0078–10.00 mg / mL) it showed a moderate reducing ability. C sinensis showed no ability to remove DPPH radicals. Conclusion. C. sinensis effectively removes hydroxyl radicals, for which the body does not have adequate antioxidant protection, so we can include it in the group of free radical scavengers., Cilj. Prooksidansi i antioksidansi utiču na oksidativnu ravnotežu na intracelularnom nivou. Oksidativni stres je posledica prekomerne produkcije prooksidanasa i nastaje usled poremećaja u ravnoteži oksido-redukcionih procesa. Neenzimski antioksidansi male molekulske mase mogu se uneti u organizam preko hrane. Cordyceps sinensis (C. sinensis) lekovita je gljiva koja se koristi u tradicionalnoj kineskoj medicini, ima bogat sadržaj vitamina, raznih polisaharida, kao i mnogih nukleozida. Cilj istraživanja ove studije bila je evaluacija antioksidativnog kapaciteta dijetetskog suplementa C. sinensis. Metode. Mereni su kapacitet „skevindžer“ aktivnosti hidroksil radikala, ukupna antioksidativna aktivnost primenom FRAP (Ferric Reducing Antioxidant Power) metode i DPPH (2,2-difenil-1-pikrilhidrazil) – skevindžer aktivnost. Rezultati. C. sinensis je u ispitivanim koncentracijama 0,0078–2,00 mg/mL imao izraženu sposobnost uklanjanja hidroskil radikala, čija je IC50 iznosila 0,5 mg/mL, dok je u koncentracijama 0,0078–10,00 mg/mL pokazao umerenu redukcionu sposobnost. C. sinensis nije pokazao sposobnost uklanjanja DPPH radikala. Zaključak. C. sinensis efikasno neutrališe hidroksilne radikale, za koje organizam nema adekvatnu antioksidativnu zaštitu pa ga možemo uvrstiti u grupu potencijalnih protektora od slobodnih radikala.",
publisher = "Serbian Medical Society",
journal = "Medicinski Casopis",
title = "Evaluation of antioxidant potential of cordyceps sinensis in vitro, Evaluacija antioksidativnog potencijala gljive Cordyceps sinensis in vitro",
volume = "53",
number = "4",
pages = "129-134",
doi = "10.5937/mckg53-24450"
}

Živković, L., Borozan, S., Bajić, V., Đorđević, S., Hristov, A.,& Spremo-Potparević, B.. (2019). Evaluation of antioxidant potential of cordyceps sinensis in vitro. in Medicinski Casopis
Serbian Medical Society., 53(4), 129-134.
https://doi.org/10.5937/mckg53-24450

Živković L, Borozan S, Bajić V, Đorđević S, Hristov A, Spremo-Potparević B. Evaluation of antioxidant potential of cordyceps sinensis in vitro. in Medicinski Casopis. 2019;53(4):129-134.
doi:10.5937/mckg53-24450 .

Živković, Lada, Borozan, Sunčica, Bajić, Vladan, Đorđević, Stefana, Hristov, Aleksandar, Spremo-Potparević, Biljana, "Evaluation of antioxidant potential of cordyceps sinensis in vitro" in Medicinski Casopis, 53, no. 4 (2019):129-134,
https://doi.org/10.5937/mckg53-24450 . .

TY  - JOUR
AU  - Živković, Lada
AU  - Bajić, Vladan
AU  - Topalović, Dijana
AU  - Bruić, Marija
AU  - Spremo-Potparević, Biljana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3484
AB  - The health benefits of natural products have long been recognized. Consumption of dietary compounds such as supplements provides an alternative source of natural products to those obtained from the diet. There is a growing concern regarding the possible side effects of using different food supplements simultaneously, since their possible interactions are less known. For the first time, we have tested genotoxic and antigenotoxic effects of Biochaga, in combination with dihydroquercetin. No genotoxic effect on whole blood cells was observed within individual treatment of Biochaga (250 μg/mL, 500 μg/mL and 1000 μg/mL) and dihydroquercetin (100 μg/mL, 250 μg/mL and 500 μg/mL), nor in combination. Afterwards, antigenotoxic potency of both supplements against hydrogen peroxide- (H2O2-) induced DNA damage to whole blood cells (WBC) was assessed, using the comet assay. Biochaga and dihydroquercetin displayed a strong potential to attenuate H2O2-induced damage on DNA in cells at all tested concentrations, with a statistical significance (p < 0:05), whereas Biochaga at the dose of 500 μg/mL in combination with dihydroquercetin 500 μg/mL was most prominent. Biochaga in combination with dihydroquercetin is able to protect genomic material from oxidative damage induced by hydrogen peroxide in vitro.
PB  - Hindawi
T2  - Oxidative Medicine and Cellular Longevity
T1  - Antigenotoxic Effects of Biochaga and Dihydroquercetin (Taxifolin) on H2O2-Induced DNA Damage in Human Whole Blood Cells
VL  - 2019
DO  - 10.1155/2019/5039372
ER  - 

@article{
author = "Živković, Lada and Bajić, Vladan and Topalović, Dijana and Bruić, Marija and Spremo-Potparević, Biljana",
year = "2019",
abstract = "The health benefits of natural products have long been recognized. Consumption of dietary compounds such as supplements provides an alternative source of natural products to those obtained from the diet. There is a growing concern regarding the possible side effects of using different food supplements simultaneously, since their possible interactions are less known. For the first time, we have tested genotoxic and antigenotoxic effects of Biochaga, in combination with dihydroquercetin. No genotoxic effect on whole blood cells was observed within individual treatment of Biochaga (250 μg/mL, 500 μg/mL and 1000 μg/mL) and dihydroquercetin (100 μg/mL, 250 μg/mL and 500 μg/mL), nor in combination. Afterwards, antigenotoxic potency of both supplements against hydrogen peroxide- (H2O2-) induced DNA damage to whole blood cells (WBC) was assessed, using the comet assay. Biochaga and dihydroquercetin displayed a strong potential to attenuate H2O2-induced damage on DNA in cells at all tested concentrations, with a statistical significance (p < 0:05), whereas Biochaga at the dose of 500 μg/mL in combination with dihydroquercetin 500 μg/mL was most prominent. Biochaga in combination with dihydroquercetin is able to protect genomic material from oxidative damage induced by hydrogen peroxide in vitro.",
publisher = "Hindawi",
journal = "Oxidative Medicine and Cellular Longevity",
title = "Antigenotoxic Effects of Biochaga and Dihydroquercetin (Taxifolin) on H2O2-Induced DNA Damage in Human Whole Blood Cells",
volume = "2019",
doi = "10.1155/2019/5039372"
}

Živković, L., Bajić, V., Topalović, D., Bruić, M.,& Spremo-Potparević, B.. (2019). Antigenotoxic Effects of Biochaga and Dihydroquercetin (Taxifolin) on H2O2-Induced DNA Damage in Human Whole Blood Cells. in Oxidative Medicine and Cellular Longevity
Hindawi., 2019.
https://doi.org/10.1155/2019/5039372

Živković L, Bajić V, Topalović D, Bruić M, Spremo-Potparević B. Antigenotoxic Effects of Biochaga and Dihydroquercetin (Taxifolin) on H2O2-Induced DNA Damage in Human Whole Blood Cells. in Oxidative Medicine and Cellular Longevity. 2019;2019.
doi:10.1155/2019/5039372 .

Živković, Lada, Bajić, Vladan, Topalović, Dijana, Bruić, Marija, Spremo-Potparević, Biljana, "Antigenotoxic Effects of Biochaga and Dihydroquercetin (Taxifolin) on H2O2-Induced DNA Damage in Human Whole Blood Cells" in Oxidative Medicine and Cellular Longevity, 2019 (2019),
https://doi.org/10.1155/2019/5039372 . .

TY  - JOUR
AU  - Živković, Lada
AU  - Bajić, Vladan
AU  - Bruić, Marija
AU  - Borozan, Sunčica
AU  - Popić, Kristina
AU  - Topalović, Dijana
AU  - Santibanez, Juan
AU  - Spremo-Potparević, Biljana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3459
AB  - Immune Assist (IA) is produced from extract of six species of medical mushrooms: Agaricus blazei - Cordyceps sinensis - Grifola frondosa - Ganoderma lucidum - Coriolus versicolor - Lentinula edodes. The genoprotective potential of IA was evaluated for the first time. Significant antigenotoxic effects were detected in human peripheral blood cells against H2O2 induced DNA damage, in the pretreatment and in the posttreatment. The most efficient concentration of IA in pretreatment was 500 μg/mL, while in posttreatment it was the concentration of 250 μg/mL. Kinetics of attenuation of H2O2 induced DNA damage in posttreatment with the optimal concentration of IA showed significant decrease in the number of damaged cells at all time periods (15–60 min), reaching the greatest reduction after 15 and 45 min. Remarkable ·OH scavenging properties and moderate reducing power, together with the modest DPPH scavenging activity, could be responsible for the great attenuation of DNA damage after 15 min of exposure to IA, while reduction of DNA damage after 45 min could be the result in additional stimulation of the cell’s repair machinery. Our results suggest that IA displayed antigenotoxic and antioxidant properties. A broader investigation of its profile in biological systems is needed.
PB  - Elsevier
T2  - Mutation Research/Genetic Toxicology and Environmental Mutagenesis
T1  - Antigenotoxic and antioxidant potential of medicinal mushrooms (Immune Assist) against DNA damage induced by free radicals-an in vitro study
VL  - 845
SP  - 1
EP  - 6
DO  - 10.1016/j.mrgentox.2019.06.008
ER  - 

@article{
author = "Živković, Lada and Bajić, Vladan and Bruić, Marija and Borozan, Sunčica and Popić, Kristina and Topalović, Dijana and Santibanez, Juan and Spremo-Potparević, Biljana",
year = "2019",
abstract = "Immune Assist (IA) is produced from extract of six species of medical mushrooms: Agaricus blazei - Cordyceps sinensis - Grifola frondosa - Ganoderma lucidum - Coriolus versicolor - Lentinula edodes. The genoprotective potential of IA was evaluated for the first time. Significant antigenotoxic effects were detected in human peripheral blood cells against H2O2 induced DNA damage, in the pretreatment and in the posttreatment. The most efficient concentration of IA in pretreatment was 500 μg/mL, while in posttreatment it was the concentration of 250 μg/mL. Kinetics of attenuation of H2O2 induced DNA damage in posttreatment with the optimal concentration of IA showed significant decrease in the number of damaged cells at all time periods (15–60 min), reaching the greatest reduction after 15 and 45 min. Remarkable ·OH scavenging properties and moderate reducing power, together with the modest DPPH scavenging activity, could be responsible for the great attenuation of DNA damage after 15 min of exposure to IA, while reduction of DNA damage after 45 min could be the result in additional stimulation of the cell’s repair machinery. Our results suggest that IA displayed antigenotoxic and antioxidant properties. A broader investigation of its profile in biological systems is needed.",
publisher = "Elsevier",
journal = "Mutation Research/Genetic Toxicology and Environmental Mutagenesis",
title = "Antigenotoxic and antioxidant potential of medicinal mushrooms (Immune Assist) against DNA damage induced by free radicals-an in vitro study",
volume = "845",
pages = "1-6",
doi = "10.1016/j.mrgentox.2019.06.008"
}

Živković, L., Bajić, V., Bruić, M., Borozan, S., Popić, K., Topalović, D., Santibanez, J.,& Spremo-Potparević, B.. (2019). Antigenotoxic and antioxidant potential of medicinal mushrooms (Immune Assist) against DNA damage induced by free radicals-an in vitro study. in Mutation Research/Genetic Toxicology and Environmental Mutagenesis
Elsevier., 845, 1-6.
https://doi.org/10.1016/j.mrgentox.2019.06.008

Živković L, Bajić V, Bruić M, Borozan S, Popić K, Topalović D, Santibanez J, Spremo-Potparević B. Antigenotoxic and antioxidant potential of medicinal mushrooms (Immune Assist) against DNA damage induced by free radicals-an in vitro study. in Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 2019;845:1-6.
doi:10.1016/j.mrgentox.2019.06.008 .

Živković, Lada, Bajić, Vladan, Bruić, Marija, Borozan, Sunčica, Popić, Kristina, Topalović, Dijana, Santibanez, Juan, Spremo-Potparević, Biljana, "Antigenotoxic and antioxidant potential of medicinal mushrooms (Immune Assist) against DNA damage induced by free radicals-an in vitro study" in Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 845 (2019):1-6,
https://doi.org/10.1016/j.mrgentox.2019.06.008 . .

TY  - JOUR
AU  - Živković, Lada
AU  - Bajić, Vladan
AU  - Bruić, Marija
AU  - Borozan, Sunčica
AU  - Popić, Kristina
AU  - Topalović, Dijana
AU  - Santibanez, Juan
AU  - Spremo-Potparević, Biljana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3456
AB  - Immune Assist (IA) is produced from extract of six species of medical mushrooms: Agaricus blazei - Cordyceps sinensis - Grifola frondosa - Ganoderma lucidum - Coriolus versicolor - Lentinula edodes. The genoprotective potential of IA was evaluated for the first time. Significant antigenotoxic effects were detected in human peripheral blood cells against H2O2 induced DNA damage, in the pretreatment and in the posttreatment. The most efficient concentration of IA in pretreatment was 500 μg/mL, while in posttreatment it was the concentration of 250 μg/mL. Kinetics of attenuation of H2O2 induced DNA damage in posttreatment with the optimal concentration of IA showed significant decrease in the number of damaged cells at all time periods (15–60 min), reaching the greatest reduction after 15 and 45 min. Remarkable ·OH scavenging properties and moderate reducing power, together with the modest DPPH scavenging activity, could be responsible for the great attenuation of DNA damage after 15 min of exposure to IA, while reduction of DNA damage after 45 min could be the result in additional stimulation of the cell’s repair machinery. Our results suggest that IA displayed antigenotoxic and antioxidant properties. A broader investigation of its profile in biological systems is needed.
PB  - Elsevier
T2  - Mutation Research/Genetic Toxicology and Environmental Mutagenesis
T1  - Antigenotoxic and antioxidant potential of medicinal mushrooms (Immune Assist) against DNA damage induced by free radicals-an in vitro study
VL  - 845
SP  - 1
EP  - 6
DO  - 10.1016/j.mrgentox.2019.06.008
ER  - 

@article{
author = "Živković, Lada and Bajić, Vladan and Bruić, Marija and Borozan, Sunčica and Popić, Kristina and Topalović, Dijana and Santibanez, Juan and Spremo-Potparević, Biljana",
year = "2019",
abstract = "Immune Assist (IA) is produced from extract of six species of medical mushrooms: Agaricus blazei - Cordyceps sinensis - Grifola frondosa - Ganoderma lucidum - Coriolus versicolor - Lentinula edodes. The genoprotective potential of IA was evaluated for the first time. Significant antigenotoxic effects were detected in human peripheral blood cells against H2O2 induced DNA damage, in the pretreatment and in the posttreatment. The most efficient concentration of IA in pretreatment was 500 μg/mL, while in posttreatment it was the concentration of 250 μg/mL. Kinetics of attenuation of H2O2 induced DNA damage in posttreatment with the optimal concentration of IA showed significant decrease in the number of damaged cells at all time periods (15–60 min), reaching the greatest reduction after 15 and 45 min. Remarkable ·OH scavenging properties and moderate reducing power, together with the modest DPPH scavenging activity, could be responsible for the great attenuation of DNA damage after 15 min of exposure to IA, while reduction of DNA damage after 45 min could be the result in additional stimulation of the cell’s repair machinery. Our results suggest that IA displayed antigenotoxic and antioxidant properties. A broader investigation of its profile in biological systems is needed.",
publisher = "Elsevier",
journal = "Mutation Research/Genetic Toxicology and Environmental Mutagenesis",
title = "Antigenotoxic and antioxidant potential of medicinal mushrooms (Immune Assist) against DNA damage induced by free radicals-an in vitro study",
volume = "845",
pages = "1-6",
doi = "10.1016/j.mrgentox.2019.06.008"
}

Živković, L., Bajić, V., Bruić, M., Borozan, S., Popić, K., Topalović, D., Santibanez, J.,& Spremo-Potparević, B.. (2019). Antigenotoxic and antioxidant potential of medicinal mushrooms (Immune Assist) against DNA damage induced by free radicals-an in vitro study. in Mutation Research/Genetic Toxicology and Environmental Mutagenesis
Elsevier., 845, 1-6.
https://doi.org/10.1016/j.mrgentox.2019.06.008

Živković L, Bajić V, Bruić M, Borozan S, Popić K, Topalović D, Santibanez J, Spremo-Potparević B. Antigenotoxic and antioxidant potential of medicinal mushrooms (Immune Assist) against DNA damage induced by free radicals-an in vitro study. in Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 2019;845:1-6.
doi:10.1016/j.mrgentox.2019.06.008 .

Živković, Lada, Bajić, Vladan, Bruić, Marija, Borozan, Sunčica, Popić, Kristina, Topalović, Dijana, Santibanez, Juan, Spremo-Potparević, Biljana, "Antigenotoxic and antioxidant potential of medicinal mushrooms (Immune Assist) against DNA damage induced by free radicals-an in vitro study" in Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 845 (2019):1-6,
https://doi.org/10.1016/j.mrgentox.2019.06.008 . .

TY  - JOUR
AU  - Topalović, Dijana
AU  - Dekanski, Dragana
AU  - Potparević, Biljana
AU  - Pirković, Andrea
AU  - Borozan, Sunčica
AU  - Bajić, Vladan
AU  - Stojanović, Danilo
AU  - Giampieri, Francesca
AU  - Gasparrini, Massimiliano
AU  - Živković, Lada
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3140
AB  - Phenolic groups of steroidal or nonsteroidal estrogens can redox cycle, leading to oxidative stress, where creation of reactive oxygen species are recognized as the main mechanism of their DNA damage properties. Dry olive (Olea europaea L.) leaf extract is known to contain bioactive and antioxidative components and to have an ability to modulate the effects of various oxidants in cells. The main goal of this study was to investigate antigenotoxic potential of a standardized dry olive leaf extract on DNA damage induced by 17β-estradiol and diethylstilbestrol in human whole blood cells in vitro, using comet assay. Our results indicated that both hormones showed a genotoxic effect at a concentration of 100 μM (P  lt  0.05, n = 6). Dry olive leaf extract was efficient in reducing number of cells with estrogen-induced DNA damage at tested concentrations (0.125, 0.5 and 1 mg/mL) (P  lt  0.05, n = 6) and under two experimental protocols, pre-treatment and post-treatment, exhibiting antigenotoxic properties. Analysis of antioxidant properties of the extract revealed moderate ABTS radical scavenging properties and reducing power. Overall, our results suggested that the protective potential of dry olive leaf extract could arise from the synergistic effect of its scavenging activity and enhancement of the cells' antioxidant capacity.
PB  - Elsevier B.V.
T2  - Mutation Research - Genetic Toxicology and Environmental Mutagenesis
T1  - Dry olive leaf extract attenuates DNA damage induced by estradiol and diethylstilbestrol in human peripheral blood cells in vitro
VL  - 845
DO  - 10.1016/j.mrgentox.2018.12.001
ER  - 

@article{
author = "Topalović, Dijana and Dekanski, Dragana and Potparević, Biljana and Pirković, Andrea and Borozan, Sunčica and Bajić, Vladan and Stojanović, Danilo and Giampieri, Francesca and Gasparrini, Massimiliano and Živković, Lada",
year = "2019",
abstract = "Phenolic groups of steroidal or nonsteroidal estrogens can redox cycle, leading to oxidative stress, where creation of reactive oxygen species are recognized as the main mechanism of their DNA damage properties. Dry olive (Olea europaea L.) leaf extract is known to contain bioactive and antioxidative components and to have an ability to modulate the effects of various oxidants in cells. The main goal of this study was to investigate antigenotoxic potential of a standardized dry olive leaf extract on DNA damage induced by 17β-estradiol and diethylstilbestrol in human whole blood cells in vitro, using comet assay. Our results indicated that both hormones showed a genotoxic effect at a concentration of 100 μM (P  lt  0.05, n = 6). Dry olive leaf extract was efficient in reducing number of cells with estrogen-induced DNA damage at tested concentrations (0.125, 0.5 and 1 mg/mL) (P  lt  0.05, n = 6) and under two experimental protocols, pre-treatment and post-treatment, exhibiting antigenotoxic properties. Analysis of antioxidant properties of the extract revealed moderate ABTS radical scavenging properties and reducing power. Overall, our results suggested that the protective potential of dry olive leaf extract could arise from the synergistic effect of its scavenging activity and enhancement of the cells' antioxidant capacity.",
publisher = "Elsevier B.V.",
journal = "Mutation Research - Genetic Toxicology and Environmental Mutagenesis",
title = "Dry olive leaf extract attenuates DNA damage induced by estradiol and diethylstilbestrol in human peripheral blood cells in vitro",
volume = "845",
doi = "10.1016/j.mrgentox.2018.12.001"
}

Topalović, D., Dekanski, D., Potparević, B., Pirković, A., Borozan, S., Bajić, V., Stojanović, D., Giampieri, F., Gasparrini, M.,& Živković, L.. (2019). Dry olive leaf extract attenuates DNA damage induced by estradiol and diethylstilbestrol in human peripheral blood cells in vitro. in Mutation Research - Genetic Toxicology and Environmental Mutagenesis
Elsevier B.V.., 845.
https://doi.org/10.1016/j.mrgentox.2018.12.001

Topalović D, Dekanski D, Potparević B, Pirković A, Borozan S, Bajić V, Stojanović D, Giampieri F, Gasparrini M, Živković L. Dry olive leaf extract attenuates DNA damage induced by estradiol and diethylstilbestrol in human peripheral blood cells in vitro. in Mutation Research - Genetic Toxicology and Environmental Mutagenesis. 2019;845.
doi:10.1016/j.mrgentox.2018.12.001 .

Topalović, Dijana, Dekanski, Dragana, Potparević, Biljana, Pirković, Andrea, Borozan, Sunčica, Bajić, Vladan, Stojanović, Danilo, Giampieri, Francesca, Gasparrini, Massimiliano, Živković, Lada, "Dry olive leaf extract attenuates DNA damage induced by estradiol and diethylstilbestrol in human peripheral blood cells in vitro" in Mutation Research - Genetic Toxicology and Environmental Mutagenesis, 845 (2019),
https://doi.org/10.1016/j.mrgentox.2018.12.001 . .

TY  - JOUR
AU  - Topalović, Dijana
AU  - Dekanski, Dragana
AU  - Potparević, Biljana
AU  - Đelić, Ninoslav
AU  - Bajić, Vladan
AU  - Živković, Lada
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3131
AB  - Harmful effects of elevated levels of catecholamines are mediated by various mechanisms, including gene transcription and formation of oxidation products. The aim of this study was to see whether the molecular mechanisms underlying the damaging action of adrenaline on DNA are mediated by reactive oxygen species (ROS). To do that, we exposed human whole blood cells to 10 mu mol L-1 adrenaline or 50 mu mol L-1 H2O2 (used as positive control) that were separately pre-treated or post-treated with 500 mu mol L-1 of quercetin, a scavenger of free radicals. Quercetin significantly reduced DNA damage in both pre- and post-treatment protocols, which suggests that adrenaline mainly acts via the production of ROS. This mechanism is also supported by gradual lowering of adrenaline and H2O2-induced DNA damage 15, 30, 45, and 60 min after treatment. Our results clearly show that DNA repair mechanisms are rather effective against ROS-mediated DNA damage induced by adrenaline.
PB  - Inst Medical Research & Occupational Health, Zagreb
T2  - Arhiv za higijenu rada i toksikologiju - Archives of Industrial Hygiene and Toxicology
T1  - Assessment of adrenaline-induced DNA damage in whole blood cells with the comet assay
VL  - 69
IS  - 4
SP  - 304
EP  - 308
DO  - 10.2478/aiht-2018-69-3154
ER  - 

@article{
author = "Topalović, Dijana and Dekanski, Dragana and Potparević, Biljana and Đelić, Ninoslav and Bajić, Vladan and Živković, Lada",
year = "2018",
abstract = "Harmful effects of elevated levels of catecholamines are mediated by various mechanisms, including gene transcription and formation of oxidation products. The aim of this study was to see whether the molecular mechanisms underlying the damaging action of adrenaline on DNA are mediated by reactive oxygen species (ROS). To do that, we exposed human whole blood cells to 10 mu mol L-1 adrenaline or 50 mu mol L-1 H2O2 (used as positive control) that were separately pre-treated or post-treated with 500 mu mol L-1 of quercetin, a scavenger of free radicals. Quercetin significantly reduced DNA damage in both pre- and post-treatment protocols, which suggests that adrenaline mainly acts via the production of ROS. This mechanism is also supported by gradual lowering of adrenaline and H2O2-induced DNA damage 15, 30, 45, and 60 min after treatment. Our results clearly show that DNA repair mechanisms are rather effective against ROS-mediated DNA damage induced by adrenaline.",
publisher = "Inst Medical Research & Occupational Health, Zagreb",
journal = "Arhiv za higijenu rada i toksikologiju - Archives of Industrial Hygiene and Toxicology",
title = "Assessment of adrenaline-induced DNA damage in whole blood cells with the comet assay",
volume = "69",
number = "4",
pages = "304-308",
doi = "10.2478/aiht-2018-69-3154"
}

Topalović, D., Dekanski, D., Potparević, B., Đelić, N., Bajić, V.,& Živković, L.. (2018). Assessment of adrenaline-induced DNA damage in whole blood cells with the comet assay. in Arhiv za higijenu rada i toksikologiju - Archives of Industrial Hygiene and Toxicology
Inst Medical Research & Occupational Health, Zagreb., 69(4), 304-308.
https://doi.org/10.2478/aiht-2018-69-3154

Topalović D, Dekanski D, Potparević B, Đelić N, Bajić V, Živković L. Assessment of adrenaline-induced DNA damage in whole blood cells with the comet assay. in Arhiv za higijenu rada i toksikologiju - Archives of Industrial Hygiene and Toxicology. 2018;69(4):304-308.
doi:10.2478/aiht-2018-69-3154 .

Topalović, Dijana, Dekanski, Dragana, Potparević, Biljana, Đelić, Ninoslav, Bajić, Vladan, Živković, Lada, "Assessment of adrenaline-induced DNA damage in whole blood cells with the comet assay" in Arhiv za higijenu rada i toksikologiju - Archives of Industrial Hygiene and Toxicology, 69, no. 4 (2018):304-308,
https://doi.org/10.2478/aiht-2018-69-3154 . .

TY  - JOUR
AU  - Živković, Lada
AU  - Bajić, Vladan
AU  - Dekanski, Dragana
AU  - Pirković, Andrea
AU  - Giampieri, Francesca
AU  - Gasparrini, Massimiliano
AU  - Mazzoni, Luca
AU  - Potparević, Biljana
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3092
AB  - Manuka honey has been widely researched regarding its biological properties, in particular its antimicrobial and antioxidant capacities. We tested the genotoxic and genoprotective properties of Manuka honey, ranging from 25-1000 mu g/mL, by performing an in vitro comet assay after exposure to human whole blood. No genotoxic effect on whole blood cells was observed within the tested concentration range (p = 0.154). Then, the antigenotoxic potency of Manuka honey against oxidative DNA damage to whole blood cells was assessed. Prior to Manuka honey treatment a modest decrease of H2O2-induced DNA damage was detected in cells, with no statistical significance (p = 0.087). Post-treatment, Manuka honey displayed a stronger potential to attenuate damaged cells at all tested concentrations, with a statistical significant difference (p  lt  0.001), where concentrations of 25 and 100 mu g/mL were most efficient. Manuka honey exhibited a marked potential to protect DNA of whole blood cells from oxidative damage induced by hydrogen peroxide in vitro.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Food and Chemical Toxicology
T1  - Manuka honey attenuates oxidative damage induced by H2O2 in human whole blood in vitro
VL  - 119
SP  - 61
EP  - 65
DO  - 10.1016/j.fct.2018.05.034
ER  - 

@article{
author = "Živković, Lada and Bajić, Vladan and Dekanski, Dragana and Pirković, Andrea and Giampieri, Francesca and Gasparrini, Massimiliano and Mazzoni, Luca and Potparević, Biljana",
year = "2018",
abstract = "Manuka honey has been widely researched regarding its biological properties, in particular its antimicrobial and antioxidant capacities. We tested the genotoxic and genoprotective properties of Manuka honey, ranging from 25-1000 mu g/mL, by performing an in vitro comet assay after exposure to human whole blood. No genotoxic effect on whole blood cells was observed within the tested concentration range (p = 0.154). Then, the antigenotoxic potency of Manuka honey against oxidative DNA damage to whole blood cells was assessed. Prior to Manuka honey treatment a modest decrease of H2O2-induced DNA damage was detected in cells, with no statistical significance (p = 0.087). Post-treatment, Manuka honey displayed a stronger potential to attenuate damaged cells at all tested concentrations, with a statistical significant difference (p  lt  0.001), where concentrations of 25 and 100 mu g/mL were most efficient. Manuka honey exhibited a marked potential to protect DNA of whole blood cells from oxidative damage induced by hydrogen peroxide in vitro.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Food and Chemical Toxicology",
title = "Manuka honey attenuates oxidative damage induced by H2O2 in human whole blood in vitro",
volume = "119",
pages = "61-65",
doi = "10.1016/j.fct.2018.05.034"
}

Živković, L., Bajić, V., Dekanski, D., Pirković, A., Giampieri, F., Gasparrini, M., Mazzoni, L.,& Potparević, B.. (2018). Manuka honey attenuates oxidative damage induced by H2O2 in human whole blood in vitro. in Food and Chemical Toxicology
Pergamon-Elsevier Science Ltd, Oxford., 119, 61-65.
https://doi.org/10.1016/j.fct.2018.05.034

Živković L, Bajić V, Dekanski D, Pirković A, Giampieri F, Gasparrini M, Mazzoni L, Potparević B. Manuka honey attenuates oxidative damage induced by H2O2 in human whole blood in vitro. in Food and Chemical Toxicology. 2018;119:61-65.
doi:10.1016/j.fct.2018.05.034 .

Živković, Lada, Bajić, Vladan, Dekanski, Dragana, Pirković, Andrea, Giampieri, Francesca, Gasparrini, Massimiliano, Mazzoni, Luca, Potparević, Biljana, "Manuka honey attenuates oxidative damage induced by H2O2 in human whole blood in vitro" in Food and Chemical Toxicology, 119 (2018):61-65,
https://doi.org/10.1016/j.fct.2018.05.034 . .

TY  - JOUR
AU  - Radaković, Milena
AU  - Borozan, Sunčica
AU  - Đelić, Ninoslav
AU  - Ivanović, Sasa
AU  - Čupić-Miladinović, Dejana
AU  - Ristanić, Marko
AU  - Potparević, Biljana
AU  - Stanimirović, Zoran
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3064
AB  - This study is aimed at analysing biochemical and genetic endpoints of toxic effects after administration of adrenaline. For this purpose, the study was carried out on Wistar rats and three doses of adrenaline were used: 0.75 mg/kg, 1.5 mg/kg, and 3 mg/kg body weight. To achieve these aims, we investigated the effects of adrenaline on catalase (CAT), Cu, Zn-superoxide dismutase (SOD), malondialdehyde (MDA), nitrite (NO2-), carbonyl groups (PCC), and nitrotyrosine (3-NT). Total activity of lactate dehydrogenase (LDH), its relative distribution (LDH1-LDH5) activity, level of acute phase proteins (APPs), and genotoxic effect were also evaluated. The obtained results revealed that all doses of adrenaline induced a significant rise in CAT activity, MDA level, PCC, NO2-, and 3-NT and a significant decrease in SOD activity compared to control. Adrenaline exerted an increase in total activity of LDH, LDH1, and LDH2 isoenzymes. Further study showed that adrenaline significantly decreased serum albumin level and albumin-globulin ratio, while the level of APPs (alpha(1) -acid glycoprotein and haptoglobulin) is increased. The micronucleus test revealed a genotoxic effect of adrenaline at higher concentrations (1.5 mg/kg and 3 mg/kg body weight) compared to untreated rats. It can be concluded that adrenaline exerts oxidative and nitrative stress in rats, increased damage to lipids and proteins, and damage of cardiomyocytes and cytogenetic damage. Obtained results may contribute to better understanding of the toxicity of adrenaline with aims to preventing its harmful effects.
PB  - Hindawi Ltd, London
T2  - Oxidative Medicine and Cellular Longevity
T1  - Nitroso-Oxidative Stress, Acute Phase Response, and Cytogenetic Damage in Wistar Rats Treated with Adrenaline
DO  - 10.1155/2018/1805354
ER  - 

@article{
author = "Radaković, Milena and Borozan, Sunčica and Đelić, Ninoslav and Ivanović, Sasa and Čupić-Miladinović, Dejana and Ristanić, Marko and Potparević, Biljana and Stanimirović, Zoran",
year = "2018",
abstract = "This study is aimed at analysing biochemical and genetic endpoints of toxic effects after administration of adrenaline. For this purpose, the study was carried out on Wistar rats and three doses of adrenaline were used: 0.75 mg/kg, 1.5 mg/kg, and 3 mg/kg body weight. To achieve these aims, we investigated the effects of adrenaline on catalase (CAT), Cu, Zn-superoxide dismutase (SOD), malondialdehyde (MDA), nitrite (NO2-), carbonyl groups (PCC), and nitrotyrosine (3-NT). Total activity of lactate dehydrogenase (LDH), its relative distribution (LDH1-LDH5) activity, level of acute phase proteins (APPs), and genotoxic effect were also evaluated. The obtained results revealed that all doses of adrenaline induced a significant rise in CAT activity, MDA level, PCC, NO2-, and 3-NT and a significant decrease in SOD activity compared to control. Adrenaline exerted an increase in total activity of LDH, LDH1, and LDH2 isoenzymes. Further study showed that adrenaline significantly decreased serum albumin level and albumin-globulin ratio, while the level of APPs (alpha(1) -acid glycoprotein and haptoglobulin) is increased. The micronucleus test revealed a genotoxic effect of adrenaline at higher concentrations (1.5 mg/kg and 3 mg/kg body weight) compared to untreated rats. It can be concluded that adrenaline exerts oxidative and nitrative stress in rats, increased damage to lipids and proteins, and damage of cardiomyocytes and cytogenetic damage. Obtained results may contribute to better understanding of the toxicity of adrenaline with aims to preventing its harmful effects.",
publisher = "Hindawi Ltd, London",
journal = "Oxidative Medicine and Cellular Longevity",
title = "Nitroso-Oxidative Stress, Acute Phase Response, and Cytogenetic Damage in Wistar Rats Treated with Adrenaline",
doi = "10.1155/2018/1805354"
}

Radaković, M., Borozan, S., Đelić, N., Ivanović, S., Čupić-Miladinović, D., Ristanić, M., Potparević, B.,& Stanimirović, Z.. (2018). Nitroso-Oxidative Stress, Acute Phase Response, and Cytogenetic Damage in Wistar Rats Treated with Adrenaline. in Oxidative Medicine and Cellular Longevity
Hindawi Ltd, London..
https://doi.org/10.1155/2018/1805354

Radaković M, Borozan S, Đelić N, Ivanović S, Čupić-Miladinović D, Ristanić M, Potparević B, Stanimirović Z. Nitroso-Oxidative Stress, Acute Phase Response, and Cytogenetic Damage in Wistar Rats Treated with Adrenaline. in Oxidative Medicine and Cellular Longevity. 2018;.
doi:10.1155/2018/1805354 .

Radaković, Milena, Borozan, Sunčica, Đelić, Ninoslav, Ivanović, Sasa, Čupić-Miladinović, Dejana, Ristanić, Marko, Potparević, Biljana, Stanimirović, Zoran, "Nitroso-Oxidative Stress, Acute Phase Response, and Cytogenetic Damage in Wistar Rats Treated with Adrenaline" in Oxidative Medicine and Cellular Longevity (2018),
https://doi.org/10.1155/2018/1805354 . .

TY  - JOUR
AU  - Janković, Milena
AU  - Živković, Lada
AU  - Pirković, Andrea
AU  - Topalović, Dijana
AU  - Dekanski, Dragana
AU  - Bajić, Vladan
AU  - Potparević, Biljana
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2941
AB  - Aim. Oxidative stress is a consequence of the increased production of free radicals that is caused by the disturbance in the balance of oxidation-reduction activity. Salvianolic acid B is a polyphenol compound, derived from the plant Salvia miltiorrhiza Bunge, which showed significant antioxidant properties. The aim of this study is the investigation of genotoxic potential of salvianolic acid B and evaluation of its antigenotoxic activity against the DNA damage induced by hydrogen peroxide in peripheral blood leukocytes in vitro, using the alkaline Comet assay. Materials and Methods. The evaluation of the ability of various concentrations of salvianolic acid B (12.5μM, 25μM and 50 μM) to reduce the number of cells with DNA damage caused by hydrogen peroxide as an oxidant was performed under two experimental protocols: pretreatment and cotreatment, in order to determine antigenotoxicity on preventive and intervention levels. Results. Results indicate that the salvianolic acid B did not exhibit a genotoxic effect after 30 minutes of incubation, in the tested concentrations. In the pretreatment, a concentration of 50 μM showed a significant decrease of the hydrogen peroxide induced DNA damage. Salvianolic acid B was more effective in reducing DNA damage in cotreatment, where concentrations of 25 μM and 50 μM demonstrated a significant abrogation of DNA damage. Protective effect of salvianolic acid B was dependent on the concentration. Conclusions. The results showed that salvianolic acid B has pronounced antigenotoxic effect on the intervention level, which makes it a potential agent in treatment of diseases in which oxidative DNA damage plays an important role.
AB  - Cilj. Oksidativni stres je posledica prekomerne produkcije slobodnih radikala i nastaje usled poremećaja u ravnoteži oksido-redukcionih procesa. Salvianolična kiselina B je polifenolno jedinjenje, poreklom iz biljke Salvia miltiorrhiza Bunge, koje je pokazalo značajna antioksidativna svojstva. Cilj rada bio je da se ispita genotoksični potencijal salvianolične kiseline B i izvrši evaluacija njene antigenotoksične aktivnosti na DNK oštećenja indukovana vodonik-peroksidom u leukocitima periferne krvi in vitro, primenom alkalnog Komet testa. Materijal i metode. Procenjena je sposobnost različitih koncentracija salvianolične kiseline B (12,5 μM, 25 μM i 50 μM) da redukuju broj ćelija sa DNK oštećenjem izazvanim vodonik-peroksidom kao oksidansom u okviru dva eksperimentalna protokola: pretretmana i kotretmana radi utvrđivanja antigenotoksičnosti na preventivnom i interventnom nivou. Rezultati. Rezultati su pokazali da salvianolična kiselina B, nakon 30 minuta inkubacije, nije ispoljila genotoksičan efekat u ispitivanim koncentracijama. U pretretmanu, koncentracija od 50 μM pokazala je značajnu redukciju DNK oštećenja indukovanih vodonik-peroksidom. Salvianolična kiselina B bila je efikasnija u redukciji DNK oštećenja u kotretmanu, gde su koncentracije od 25 μM i 50 μM pokazale značajan efekat redukcije nivoa DNK oštećenja. Pokazani protektivni efekat salvianolične kiseline B zavisio je od koncentracije. Zaključak. Dobijeni rezultati pokazali su da salvianolična kiselina B ima izraženiji antigenotoksični efekat na interventnom nivou, što je čini potencijalnim agensom za primenu kod oboljenja u kojima oksidativna DNK oštećenja imaju važnu ulogu.
PB  - Srpsko lekarsko društvo - Okružna podružnica Kragujevac, Kragujevac
T2  - Medicinski časopis
T1  - Evaluation of antigenotoxic potential of salvianolic acid B with hydrogen peroxide on human peripheral blood leukocytes in vitro
T1  - Evaluacija antigenotoksičnog potencijala salvianolične kiseline B u prisustvu vodonik-peroksida na leukocitima periferne krvi in vitro
VL  - 51
IS  - 2
SP  - 39
EP  - 45
DO  - 10.5937/mckg51-15559
ER  - 

@article{
author = "Janković, Milena and Živković, Lada and Pirković, Andrea and Topalović, Dijana and Dekanski, Dragana and Bajić, Vladan and Potparević, Biljana",
year = "2017",
abstract = "Aim. Oxidative stress is a consequence of the increased production of free radicals that is caused by the disturbance in the balance of oxidation-reduction activity. Salvianolic acid B is a polyphenol compound, derived from the plant Salvia miltiorrhiza Bunge, which showed significant antioxidant properties. The aim of this study is the investigation of genotoxic potential of salvianolic acid B and evaluation of its antigenotoxic activity against the DNA damage induced by hydrogen peroxide in peripheral blood leukocytes in vitro, using the alkaline Comet assay. Materials and Methods. The evaluation of the ability of various concentrations of salvianolic acid B (12.5μM, 25μM and 50 μM) to reduce the number of cells with DNA damage caused by hydrogen peroxide as an oxidant was performed under two experimental protocols: pretreatment and cotreatment, in order to determine antigenotoxicity on preventive and intervention levels. Results. Results indicate that the salvianolic acid B did not exhibit a genotoxic effect after 30 minutes of incubation, in the tested concentrations. In the pretreatment, a concentration of 50 μM showed a significant decrease of the hydrogen peroxide induced DNA damage. Salvianolic acid B was more effective in reducing DNA damage in cotreatment, where concentrations of 25 μM and 50 μM demonstrated a significant abrogation of DNA damage. Protective effect of salvianolic acid B was dependent on the concentration. Conclusions. The results showed that salvianolic acid B has pronounced antigenotoxic effect on the intervention level, which makes it a potential agent in treatment of diseases in which oxidative DNA damage plays an important role., Cilj. Oksidativni stres je posledica prekomerne produkcije slobodnih radikala i nastaje usled poremećaja u ravnoteži oksido-redukcionih procesa. Salvianolična kiselina B je polifenolno jedinjenje, poreklom iz biljke Salvia miltiorrhiza Bunge, koje je pokazalo značajna antioksidativna svojstva. Cilj rada bio je da se ispita genotoksični potencijal salvianolične kiseline B i izvrši evaluacija njene antigenotoksične aktivnosti na DNK oštećenja indukovana vodonik-peroksidom u leukocitima periferne krvi in vitro, primenom alkalnog Komet testa. Materijal i metode. Procenjena je sposobnost različitih koncentracija salvianolične kiseline B (12,5 μM, 25 μM i 50 μM) da redukuju broj ćelija sa DNK oštećenjem izazvanim vodonik-peroksidom kao oksidansom u okviru dva eksperimentalna protokola: pretretmana i kotretmana radi utvrđivanja antigenotoksičnosti na preventivnom i interventnom nivou. Rezultati. Rezultati su pokazali da salvianolična kiselina B, nakon 30 minuta inkubacije, nije ispoljila genotoksičan efekat u ispitivanim koncentracijama. U pretretmanu, koncentracija od 50 μM pokazala je značajnu redukciju DNK oštećenja indukovanih vodonik-peroksidom. Salvianolična kiselina B bila je efikasnija u redukciji DNK oštećenja u kotretmanu, gde su koncentracije od 25 μM i 50 μM pokazale značajan efekat redukcije nivoa DNK oštećenja. Pokazani protektivni efekat salvianolične kiseline B zavisio je od koncentracije. Zaključak. Dobijeni rezultati pokazali su da salvianolična kiselina B ima izraženiji antigenotoksični efekat na interventnom nivou, što je čini potencijalnim agensom za primenu kod oboljenja u kojima oksidativna DNK oštećenja imaju važnu ulogu.",
publisher = "Srpsko lekarsko društvo - Okružna podružnica Kragujevac, Kragujevac",
journal = "Medicinski časopis",
title = "Evaluation of antigenotoxic potential of salvianolic acid B with hydrogen peroxide on human peripheral blood leukocytes in vitro, Evaluacija antigenotoksičnog potencijala salvianolične kiseline B u prisustvu vodonik-peroksida na leukocitima periferne krvi in vitro",
volume = "51",
number = "2",
pages = "39-45",
doi = "10.5937/mckg51-15559"
}

Janković, M., Živković, L., Pirković, A., Topalović, D., Dekanski, D., Bajić, V.,& Potparević, B.. (2017). Evaluation of antigenotoxic potential of salvianolic acid B with hydrogen peroxide on human peripheral blood leukocytes in vitro. in Medicinski časopis
Srpsko lekarsko društvo - Okružna podružnica Kragujevac, Kragujevac., 51(2), 39-45.
https://doi.org/10.5937/mckg51-15559

Janković M, Živković L, Pirković A, Topalović D, Dekanski D, Bajić V, Potparević B. Evaluation of antigenotoxic potential of salvianolic acid B with hydrogen peroxide on human peripheral blood leukocytes in vitro. in Medicinski časopis. 2017;51(2):39-45.
doi:10.5937/mckg51-15559 .

Janković, Milena, Živković, Lada, Pirković, Andrea, Topalović, Dijana, Dekanski, Dragana, Bajić, Vladan, Potparević, Biljana, "Evaluation of antigenotoxic potential of salvianolic acid B with hydrogen peroxide on human peripheral blood leukocytes in vitro" in Medicinski časopis, 51, no. 2 (2017):39-45,
https://doi.org/10.5937/mckg51-15559 . .

TY  - JOUR
AU  - Živković, Lada
AU  - Borozan, Sunčica
AU  - Pirković, Andrea
AU  - Topalović, Dijana
AU  - Ciptasari, Ummi
AU  - Bajić, Vladan
AU  - Potparević, Biljana
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2906
AB  - The ability of Agaricus blazei mushroom in its dried and powdered mycelial form was evaluated for its antigenotoxic properties for the first time. Antigenotoxic effects in human peripheral blood cells against H2O2-induced DNA damage were examined in pretreatment and posttreatment protocol by comet assay. The results showed better antigenotoxic properties of Agaricus blazei on the interventional level, respectively, after treatment. Agaricus blazei in concentration of 250 mu g/mL after treatment was most efficient in regard to its action against DNA damage. The evaluation of repair kinetics showed decrease in H2O2 induced DNA damage 15min after the application of A. blazei, reaching the maximum potency after 30 min. Analysis of antioxidant properties of Agaricus blazei revealed strong center dot OH scavenging properties and moderate reducing power, while its DPPH scavenging ability was weak. In regard to our findings, we can conclude that our preliminary results demonstrated antigenotoxic properties of Agaricus blazei and its strong center dot OH scavenging ability. Mechanisms underlying its properties should be further evaluated in in vivo studies.
PB  - Hindawi Ltd, London
T2  - Oxidative Medicine and Cellular Longevity
T1  - Antigenotoxic Properties of Agaricus blazei against Hydrogen Peroxide in Human Peripheral Blood Cells
DO  - 10.1155/2017/8759764
ER  - 

@article{
author = "Živković, Lada and Borozan, Sunčica and Pirković, Andrea and Topalović, Dijana and Ciptasari, Ummi and Bajić, Vladan and Potparević, Biljana",
year = "2017",
abstract = "The ability of Agaricus blazei mushroom in its dried and powdered mycelial form was evaluated for its antigenotoxic properties for the first time. Antigenotoxic effects in human peripheral blood cells against H2O2-induced DNA damage were examined in pretreatment and posttreatment protocol by comet assay. The results showed better antigenotoxic properties of Agaricus blazei on the interventional level, respectively, after treatment. Agaricus blazei in concentration of 250 mu g/mL after treatment was most efficient in regard to its action against DNA damage. The evaluation of repair kinetics showed decrease in H2O2 induced DNA damage 15min after the application of A. blazei, reaching the maximum potency after 30 min. Analysis of antioxidant properties of Agaricus blazei revealed strong center dot OH scavenging properties and moderate reducing power, while its DPPH scavenging ability was weak. In regard to our findings, we can conclude that our preliminary results demonstrated antigenotoxic properties of Agaricus blazei and its strong center dot OH scavenging ability. Mechanisms underlying its properties should be further evaluated in in vivo studies.",
publisher = "Hindawi Ltd, London",
journal = "Oxidative Medicine and Cellular Longevity",
title = "Antigenotoxic Properties of Agaricus blazei against Hydrogen Peroxide in Human Peripheral Blood Cells",
doi = "10.1155/2017/8759764"
}

Živković, L., Borozan, S., Pirković, A., Topalović, D., Ciptasari, U., Bajić, V.,& Potparević, B.. (2017). Antigenotoxic Properties of Agaricus blazei against Hydrogen Peroxide in Human Peripheral Blood Cells. in Oxidative Medicine and Cellular Longevity
Hindawi Ltd, London..
https://doi.org/10.1155/2017/8759764

Živković L, Borozan S, Pirković A, Topalović D, Ciptasari U, Bajić V, Potparević B. Antigenotoxic Properties of Agaricus blazei against Hydrogen Peroxide in Human Peripheral Blood Cells. in Oxidative Medicine and Cellular Longevity. 2017;.
doi:10.1155/2017/8759764 .

Živković, Lada, Borozan, Sunčica, Pirković, Andrea, Topalović, Dijana, Ciptasari, Ummi, Bajić, Vladan, Potparević, Biljana, "Antigenotoxic Properties of Agaricus blazei against Hydrogen Peroxide in Human Peripheral Blood Cells" in Oxidative Medicine and Cellular Longevity (2017),
https://doi.org/10.1155/2017/8759764 . .

TY  - JOUR
AU  - Pirković, Andrea
AU  - Dekanski, Dragana
AU  - Živković, Lada
AU  - Milanović-Čabarkapa, Mirjana
AU  - Bajić, Vladan
AU  - Topalović, Dijana
AU  - Giampieri, Francesca
AU  - Gasparrini, Massimiliano
AU  - Battino, Maurizio
AU  - Potparević, Biljana
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2822
AB  - The CaNa(2)EDTA chelation therapy is often practiced with antioxidant supplementation. Dry olive leaf extract (DOLE) is natural product with antioxidant and DNA protective properties. The effects of DOLE on the levels of DNA damage were investigated ex vivo in peripheral blood lymphocytes (PBLs) of 19 workers occupationally exposed to lead (Pb), before and after CaNa(2)EDTA chelation therapy. POLE demonstrated pronounced radical scavenging activity in concentrations >= 1 mg/mL, and showed no genotoxicity per se, in concentrations 0.125-1 mg/mL. The level of DNA damage in PBLs of workers before chelation therapy was elevated (24.21 +/- 14.26) compared to controls (6.0 +/- 3.37). The incubation of PBLs before chelation therapy with selected concentration of DOLE lead to a severe increase of DNA damage (64.03 +/- 20.96), exhibiting prooxidant rather than antioxidant effect. After the five-day CaNa2EDTA chelation regimen, DNA damage in PBLs of workers decreased (8.26 +/- 4.62) significantly compared to baseline. Treatment of PBLs with DOLE after chelation, again produced high level of damage (41.82 +/- 23.17) and the acute prooxidant effects of DOLE remained, but, DNA damage was less severe than before chelation. The DOLE exhibits prooxidant effect in presence of Pb in lymphocytes of exposed workers, and its effect is less pronounced following the removal of Pb after standard chelation therapy.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Food and Chemical Toxicology
T1  - Unexpected effect of dry olive leaf extract on the level of DNA damage in lymphocytes of lead intoxicated workers, before and after CaNa(2)EDTA chelation therapy
VL  - 106
SP  - 616
EP  - 623
DO  - 10.1016/j.fct.2016.12.023
ER  - 

@article{
author = "Pirković, Andrea and Dekanski, Dragana and Živković, Lada and Milanović-Čabarkapa, Mirjana and Bajić, Vladan and Topalović, Dijana and Giampieri, Francesca and Gasparrini, Massimiliano and Battino, Maurizio and Potparević, Biljana",
year = "2017",
abstract = "The CaNa(2)EDTA chelation therapy is often practiced with antioxidant supplementation. Dry olive leaf extract (DOLE) is natural product with antioxidant and DNA protective properties. The effects of DOLE on the levels of DNA damage were investigated ex vivo in peripheral blood lymphocytes (PBLs) of 19 workers occupationally exposed to lead (Pb), before and after CaNa(2)EDTA chelation therapy. POLE demonstrated pronounced radical scavenging activity in concentrations >= 1 mg/mL, and showed no genotoxicity per se, in concentrations 0.125-1 mg/mL. The level of DNA damage in PBLs of workers before chelation therapy was elevated (24.21 +/- 14.26) compared to controls (6.0 +/- 3.37). The incubation of PBLs before chelation therapy with selected concentration of DOLE lead to a severe increase of DNA damage (64.03 +/- 20.96), exhibiting prooxidant rather than antioxidant effect. After the five-day CaNa2EDTA chelation regimen, DNA damage in PBLs of workers decreased (8.26 +/- 4.62) significantly compared to baseline. Treatment of PBLs with DOLE after chelation, again produced high level of damage (41.82 +/- 23.17) and the acute prooxidant effects of DOLE remained, but, DNA damage was less severe than before chelation. The DOLE exhibits prooxidant effect in presence of Pb in lymphocytes of exposed workers, and its effect is less pronounced following the removal of Pb after standard chelation therapy.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Food and Chemical Toxicology",
title = "Unexpected effect of dry olive leaf extract on the level of DNA damage in lymphocytes of lead intoxicated workers, before and after CaNa(2)EDTA chelation therapy",
volume = "106",
pages = "616-623",
doi = "10.1016/j.fct.2016.12.023"
}

Pirković, A., Dekanski, D., Živković, L., Milanović-Čabarkapa, M., Bajić, V., Topalović, D., Giampieri, F., Gasparrini, M., Battino, M.,& Potparević, B.. (2017). Unexpected effect of dry olive leaf extract on the level of DNA damage in lymphocytes of lead intoxicated workers, before and after CaNa(2)EDTA chelation therapy. in Food and Chemical Toxicology
Pergamon-Elsevier Science Ltd, Oxford., 106, 616-623.
https://doi.org/10.1016/j.fct.2016.12.023

Pirković A, Dekanski D, Živković L, Milanović-Čabarkapa M, Bajić V, Topalović D, Giampieri F, Gasparrini M, Battino M, Potparević B. Unexpected effect of dry olive leaf extract on the level of DNA damage in lymphocytes of lead intoxicated workers, before and after CaNa(2)EDTA chelation therapy. in Food and Chemical Toxicology. 2017;106:616-623.
doi:10.1016/j.fct.2016.12.023 .

Pirković, Andrea, Dekanski, Dragana, Živković, Lada, Milanović-Čabarkapa, Mirjana, Bajić, Vladan, Topalović, Dijana, Giampieri, Francesca, Gasparrini, Massimiliano, Battino, Maurizio, Potparević, Biljana, "Unexpected effect of dry olive leaf extract on the level of DNA damage in lymphocytes of lead intoxicated workers, before and after CaNa(2)EDTA chelation therapy" in Food and Chemical Toxicology, 106 (2017):616-623,
https://doi.org/10.1016/j.fct.2016.12.023 . .

TY  - JOUR
AU  - Živković, Lada
AU  - Pirković, Andrea
AU  - Marčetić, Mirjana
AU  - Kovačević, Nada
AU  - Bajić, Vladan
AU  - Jovičić, Snežana
AU  - Potparević, Biljana
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2538
AB  - The essential oils of genus Seseli are known for their beneficial biological activities and could present novel targets in the development of safe and effective preparations of plant products. The objective was to test the essential oils of different parts of Seseli rigidum from two natural habitats for potential genotoxic and antigenotoxic activities against H2O2-induced DNA damage in human whole blood cells in vitro, by the comet assay. The essential oil analysis showed a high falcarinol content in oil from the root, while oils of the fruit and aerial parts contained a-pinene as the main compound. Genotoxicity was not detected at any of the concentrations of the essential oils from the three parts of the plant from localities I and II. Although the antioxidant activity (established by the FRAP and DPPH tests) of the investigated oils was low, all oils demonstrated a strong antigenotoxic effect against H2O2-induced damage post-treatment, when the oils were applied after the oxidant. Based on the lack of pretreatment activity and the post-treatment reduction in DNA damage, the antigenotoxic effect of S. rigidum essential oils was probably based on the stimulation of DNA repair mechanisms. Environmental conditions did not affect the antigenotoxic properties of the oils. In conclusion, our results revealed the antigenotoxic properties of S. rigidum essential oils and appropriate and safe doses with beneficial effects under the described conditions.
PB  - Srpsko biološko društvo, Beograd, i dr.
T2  - Archives of Biological Sciences
T1  - Evaluation of genotoxic and antigenotoxic properties of essential oils of Seseli rigidum Waldst. & Kit. (Apiaceae)
VL  - 68
IS  - 1
SP  - 135
EP  - 144
DO  - 10.2298/ABS150512135Z
ER  - 

@article{
author = "Živković, Lada and Pirković, Andrea and Marčetić, Mirjana and Kovačević, Nada and Bajić, Vladan and Jovičić, Snežana and Potparević, Biljana",
year = "2016",
abstract = "The essential oils of genus Seseli are known for their beneficial biological activities and could present novel targets in the development of safe and effective preparations of plant products. The objective was to test the essential oils of different parts of Seseli rigidum from two natural habitats for potential genotoxic and antigenotoxic activities against H2O2-induced DNA damage in human whole blood cells in vitro, by the comet assay. The essential oil analysis showed a high falcarinol content in oil from the root, while oils of the fruit and aerial parts contained a-pinene as the main compound. Genotoxicity was not detected at any of the concentrations of the essential oils from the three parts of the plant from localities I and II. Although the antioxidant activity (established by the FRAP and DPPH tests) of the investigated oils was low, all oils demonstrated a strong antigenotoxic effect against H2O2-induced damage post-treatment, when the oils were applied after the oxidant. Based on the lack of pretreatment activity and the post-treatment reduction in DNA damage, the antigenotoxic effect of S. rigidum essential oils was probably based on the stimulation of DNA repair mechanisms. Environmental conditions did not affect the antigenotoxic properties of the oils. In conclusion, our results revealed the antigenotoxic properties of S. rigidum essential oils and appropriate and safe doses with beneficial effects under the described conditions.",
publisher = "Srpsko biološko društvo, Beograd, i dr.",
journal = "Archives of Biological Sciences",
title = "Evaluation of genotoxic and antigenotoxic properties of essential oils of Seseli rigidum Waldst. & Kit. (Apiaceae)",
volume = "68",
number = "1",
pages = "135-144",
doi = "10.2298/ABS150512135Z"
}

Živković, L., Pirković, A., Marčetić, M., Kovačević, N., Bajić, V., Jovičić, S.,& Potparević, B.. (2016). Evaluation of genotoxic and antigenotoxic properties of essential oils of Seseli rigidum Waldst. & Kit. (Apiaceae). in Archives of Biological Sciences
Srpsko biološko društvo, Beograd, i dr.., 68(1), 135-144.
https://doi.org/10.2298/ABS150512135Z

Živković L, Pirković A, Marčetić M, Kovačević N, Bajić V, Jovičić S, Potparević B. Evaluation of genotoxic and antigenotoxic properties of essential oils of Seseli rigidum Waldst. & Kit. (Apiaceae). in Archives of Biological Sciences. 2016;68(1):135-144.
doi:10.2298/ABS150512135Z .

Živković, Lada, Pirković, Andrea, Marčetić, Mirjana, Kovačević, Nada, Bajić, Vladan, Jovičić, Snežana, Potparević, Biljana, "Evaluation of genotoxic and antigenotoxic properties of essential oils of Seseli rigidum Waldst. & Kit. (Apiaceae)" in Archives of Biological Sciences, 68, no. 1 (2016):135-144,
https://doi.org/10.2298/ABS150512135Z . .

TY  - JOUR
AU  - Vasiljević, Jovana
AU  - Živković, Lada
AU  - Čabarkapa, Andrea
AU  - Bajić, Vladan
AU  - Đelić, Ninoslav
AU  - Potparević, Biljana
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2635
AB  - Context Cordyceps sinensis (C sinensis) is a well-known, traditional, Chinese medicinal mushroom, valued for its beneficial properties for human health. C sinensis has been reported to have immunomodulatory, anticancer, antiaging, antioxidant and anti-inflammatory activity. Despite potential medicinal benefits, no previously published reports are available about the genotoxicity or antigenotoxicity of C sinensis, as detected by comet assay. Objective The objective of the study was to evaluate both the genotoxic and antigenotoxic potential of an extract of C sinensis (CS extract) in human peripheral blood cells. Design The research team designed a pilot study. Setting The study was conducted at the Center for Biological Research, University of Belgrade, in Belgrade, Serbia. Participants Participants were 6 healthy individuals (2 males and 4 females), between the ages of 20 and 45 y, recruited on a voluntary basis, who provided heparinized, peripheral blood samples. Intervention Four concentrations of the CS extract125 mu g/mL, 250 mu g/mL, 500 mu g/mL, and 1000 mu g/mL-were used in the treatment of tested blood cells from the blood samples. Three independent procedures were performed: (1) a genotoxicity assessment, (2) an antigenotoxicity assessment for pretreatment of human cells with the CS extract prior to their exposure to hydrogen peroxide (H2O2) (ie, an evaluation of the benefits of the CS extract as a preventive agent); and (3) posttreatment of human cells with the CS extract after their exposure to H2O2 (ie, an evaluation of the benefits of the CS extract as an interventional agent). Outcome Measures Cells were graded by eye inspection into 5 classes, depending on the extent of DNA damage, representing: (1) class A-undamaged cells with no tail ( lt 5% damaged DNA); (2) class B-low-level damage (5%-20%); (3) class C-medium-level damage (20%-40%); (4) class D-high-level damage (40%-95%), and (5) class E-total destruction (>95%). Results The CS extract proved to be nongenotoxic because no induced DNA damage was detected at all tested concentrations. For the antigenotoxicity assessment of the pretreatment with the CS extract, only the 1000-mu g/mL concentration showed a significant decrease in the number of cells exhibiting H2O2-induced DNA damage. For the posttreatment, the CS extract exhibited antigenotoxic potential by attenuating H2O2-induced DNA damage at all concentrations tested. The evaluation of repair kinetics showed a decrease in DNA-damaged cells 15 min after the application of the CS extract, reaching a maximum potency after 45 min. Conclusions The results indicated that C sinensis can be used as a postapplicative agent that counteracts the effect of oxidative stress. The resulting reduction in DNA damage might be related to its scavenging properties and stimulation of DNA repair.
PB  - Innovision Communications, Aliso Viejo
T2  - Alternative Therapies in Health and Medicine
T1  - Cordyceps sinensis: Genotoxic Potential in Human Peripheral Blood Cells and Antigenotoxic Properties Against Hydrogen Peroxide by Comet Assay
VL  - 22
SP  - 24
EP  - 31
UR  - https://hdl.handle.net/21.15107/rcub_vinar_1311
ER  - 

@article{
author = "Vasiljević, Jovana and Živković, Lada and Čabarkapa, Andrea and Bajić, Vladan and Đelić, Ninoslav and Potparević, Biljana",
year = "2016",
abstract = "Context Cordyceps sinensis (C sinensis) is a well-known, traditional, Chinese medicinal mushroom, valued for its beneficial properties for human health. C sinensis has been reported to have immunomodulatory, anticancer, antiaging, antioxidant and anti-inflammatory activity. Despite potential medicinal benefits, no previously published reports are available about the genotoxicity or antigenotoxicity of C sinensis, as detected by comet assay. Objective The objective of the study was to evaluate both the genotoxic and antigenotoxic potential of an extract of C sinensis (CS extract) in human peripheral blood cells. Design The research team designed a pilot study. Setting The study was conducted at the Center for Biological Research, University of Belgrade, in Belgrade, Serbia. Participants Participants were 6 healthy individuals (2 males and 4 females), between the ages of 20 and 45 y, recruited on a voluntary basis, who provided heparinized, peripheral blood samples. Intervention Four concentrations of the CS extract125 mu g/mL, 250 mu g/mL, 500 mu g/mL, and 1000 mu g/mL-were used in the treatment of tested blood cells from the blood samples. Three independent procedures were performed: (1) a genotoxicity assessment, (2) an antigenotoxicity assessment for pretreatment of human cells with the CS extract prior to their exposure to hydrogen peroxide (H2O2) (ie, an evaluation of the benefits of the CS extract as a preventive agent); and (3) posttreatment of human cells with the CS extract after their exposure to H2O2 (ie, an evaluation of the benefits of the CS extract as an interventional agent). Outcome Measures Cells were graded by eye inspection into 5 classes, depending on the extent of DNA damage, representing: (1) class A-undamaged cells with no tail ( lt 5% damaged DNA); (2) class B-low-level damage (5%-20%); (3) class C-medium-level damage (20%-40%); (4) class D-high-level damage (40%-95%), and (5) class E-total destruction (>95%). Results The CS extract proved to be nongenotoxic because no induced DNA damage was detected at all tested concentrations. For the antigenotoxicity assessment of the pretreatment with the CS extract, only the 1000-mu g/mL concentration showed a significant decrease in the number of cells exhibiting H2O2-induced DNA damage. For the posttreatment, the CS extract exhibited antigenotoxic potential by attenuating H2O2-induced DNA damage at all concentrations tested. The evaluation of repair kinetics showed a decrease in DNA-damaged cells 15 min after the application of the CS extract, reaching a maximum potency after 45 min. Conclusions The results indicated that C sinensis can be used as a postapplicative agent that counteracts the effect of oxidative stress. The resulting reduction in DNA damage might be related to its scavenging properties and stimulation of DNA repair.",
publisher = "Innovision Communications, Aliso Viejo",
journal = "Alternative Therapies in Health and Medicine",
title = "Cordyceps sinensis: Genotoxic Potential in Human Peripheral Blood Cells and Antigenotoxic Properties Against Hydrogen Peroxide by Comet Assay",
volume = "22",
pages = "24-31",
url = "https://hdl.handle.net/21.15107/rcub_vinar_1311"
}

Vasiljević, J., Živković, L., Čabarkapa, A., Bajić, V., Đelić, N.,& Potparević, B.. (2016). Cordyceps sinensis: Genotoxic Potential in Human Peripheral Blood Cells and Antigenotoxic Properties Against Hydrogen Peroxide by Comet Assay. in Alternative Therapies in Health and Medicine
Innovision Communications, Aliso Viejo., 22, 24-31.
https://hdl.handle.net/21.15107/rcub_vinar_1311

Vasiljević J, Živković L, Čabarkapa A, Bajić V, Đelić N, Potparević B. Cordyceps sinensis: Genotoxic Potential in Human Peripheral Blood Cells and Antigenotoxic Properties Against Hydrogen Peroxide by Comet Assay. in Alternative Therapies in Health and Medicine. 2016;22:24-31.
https://hdl.handle.net/21.15107/rcub_vinar_1311 .

Vasiljević, Jovana, Živković, Lada, Čabarkapa, Andrea, Bajić, Vladan, Đelić, Ninoslav, Potparević, Biljana, "Cordyceps sinensis: Genotoxic Potential in Human Peripheral Blood Cells and Antigenotoxic Properties Against Hydrogen Peroxide by Comet Assay" in Alternative Therapies in Health and Medicine, 22 (2016):24-31,
https://hdl.handle.net/21.15107/rcub_vinar_1311 .

TY  - JOUR
AU  - Pirković, Andrea
AU  - Živković, Lada
AU  - Borozan, Sunčica
AU  - Zlatković-Svenda, Mirjana
AU  - Dekanski, Dragana
AU  - Jančić, Ivan
AU  - Radak-Perović, Marija
AU  - Bajić, Vladan
AU  - Potparević, Biljana
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2584
AB  - The effects of co-administration of dry olive leaf extract (DOLE) with standard methotrexate (MTX) therapy on the parameters of cell damage and inflammation in patients with early and long-term rheumatoid arthritis (RA) were evaluated at baseline, 3 and 6weeks. Patients were assigned to groups: the early phase RA group on MTX monotherapy (E MTX), and the two RA groups that received co-treatment with DOLE and MTX: early (E MTX+DOLE) and long-term phase patients (L-t MTX+ DOLE). Baseline values indicated increased parameters of cell damage and disruption of redox balance in all groups. After three weeks the E MTX+DOLE group maintained high catalase activity, exhibited decrease of lipid peroxidation and protein damage indicatorsthiols and nitrites, while levels of DNA damage and pro-inflammatory interleukin-6 were significantly reduced. In E MTX group catalase activity remained unaltered while significant lipid peroxidation and DNA damage reductions were seen only after six weeks. L-t MTX+DOLE group showed only modest alterations of cell damage parameters during six weeks. Combined administration of DOLE with MTX contributes to faster reduction of cell damage, restores oxidative balance and improves interleukin-6 suppression during high disease activity in early phase RA, but not in long term patients. Copyright
PB  - Wiley-Blackwell, Hoboken
T2  - Phytotherapy Research
T1  - Dry Olive Leaf Extract in Combination with Methotrexate Reduces Cell Damage in Early Rheumatoid Arthritis PatientsA Pilot Study
VL  - 30
IS  - 10
SP  - 1615
EP  - 1623
DO  - 10.1002/ptr.5662
ER  - 

@article{
author = "Pirković, Andrea and Živković, Lada and Borozan, Sunčica and Zlatković-Svenda, Mirjana and Dekanski, Dragana and Jančić, Ivan and Radak-Perović, Marija and Bajić, Vladan and Potparević, Biljana",
year = "2016",
abstract = "The effects of co-administration of dry olive leaf extract (DOLE) with standard methotrexate (MTX) therapy on the parameters of cell damage and inflammation in patients with early and long-term rheumatoid arthritis (RA) were evaluated at baseline, 3 and 6weeks. Patients were assigned to groups: the early phase RA group on MTX monotherapy (E MTX), and the two RA groups that received co-treatment with DOLE and MTX: early (E MTX+DOLE) and long-term phase patients (L-t MTX+ DOLE). Baseline values indicated increased parameters of cell damage and disruption of redox balance in all groups. After three weeks the E MTX+DOLE group maintained high catalase activity, exhibited decrease of lipid peroxidation and protein damage indicatorsthiols and nitrites, while levels of DNA damage and pro-inflammatory interleukin-6 were significantly reduced. In E MTX group catalase activity remained unaltered while significant lipid peroxidation and DNA damage reductions were seen only after six weeks. L-t MTX+DOLE group showed only modest alterations of cell damage parameters during six weeks. Combined administration of DOLE with MTX contributes to faster reduction of cell damage, restores oxidative balance and improves interleukin-6 suppression during high disease activity in early phase RA, but not in long term patients. Copyright",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Phytotherapy Research",
title = "Dry Olive Leaf Extract in Combination with Methotrexate Reduces Cell Damage in Early Rheumatoid Arthritis PatientsA Pilot Study",
volume = "30",
number = "10",
pages = "1615-1623",
doi = "10.1002/ptr.5662"
}

Pirković, A., Živković, L., Borozan, S., Zlatković-Svenda, M., Dekanski, D., Jančić, I., Radak-Perović, M., Bajić, V.,& Potparević, B.. (2016). Dry Olive Leaf Extract in Combination with Methotrexate Reduces Cell Damage in Early Rheumatoid Arthritis PatientsA Pilot Study. in Phytotherapy Research
Wiley-Blackwell, Hoboken., 30(10), 1615-1623.
https://doi.org/10.1002/ptr.5662

Pirković A, Živković L, Borozan S, Zlatković-Svenda M, Dekanski D, Jančić I, Radak-Perović M, Bajić V, Potparević B. Dry Olive Leaf Extract in Combination with Methotrexate Reduces Cell Damage in Early Rheumatoid Arthritis PatientsA Pilot Study. in Phytotherapy Research. 2016;30(10):1615-1623.
doi:10.1002/ptr.5662 .

Pirković, Andrea, Živković, Lada, Borozan, Sunčica, Zlatković-Svenda, Mirjana, Dekanski, Dragana, Jančić, Ivan, Radak-Perović, Marija, Bajić, Vladan, Potparević, Biljana, "Dry Olive Leaf Extract in Combination with Methotrexate Reduces Cell Damage in Early Rheumatoid Arthritis PatientsA Pilot Study" in Phytotherapy Research, 30, no. 10 (2016):1615-1623,
https://doi.org/10.1002/ptr.5662 . .

TY  - JOUR
AU  - Čabarkapa, Andrea
AU  - Borozan, Sunčica
AU  - Živković, Lada
AU  - Milanović-Čabarkapa, Mirjana
AU  - Stojanović, Srđan
AU  - Bajić, Vladan
AU  - Spremo-Potparević, Biljana
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2440
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3428
AB  - The aim of this study was to determine oxidative alterations leading to cellular dysfunctions in Pb-exposed subjects by evaluating damage to all major classes of biomolecules in the cell, lipid peroxidation, protein and DNA damage and determine relationships between parameters of Pb toxicity and specific biomarkers of oxidative damage.Analysis was conducted of smelter workers with high blood Pb and urine aminolevulinic acid levels and slightly elevated values of coproporphyrin and erythrocyte protoporphyrin IX. Significant decreases of thiol groups and increases in carbonyl groups as protein degradation end products, and of nitrite were detected. Elevated rates of lipid peroxidation and rises in the activities of the antioxidant enzymes Cu-Zn superoxide dismutase and catalase were also observed. Both enzymes showed positive correlations with the blood lead levels and urine coproporphyrin, while thiol groups correlated negatively with the same indices. The genotoxic potential of lead was manifested through an increased number of DNA-damaged cells. Increased activities of serum lactate dehydrogenase isoenzymes indicated cellular damage in the lungs, kidneys, and liver. These lead-induced impairments should be taken into consideration in the assessment of Pb-related health hazards.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Toxicology
T1  - Implications of oxidative stress in occupational exposure to lead on a cellular level
VL  - 97
IS  - 6
SP  - 799
EP  - 813
DO  - 10.1080/02772248.2015.1060973
ER  - 

@article{
author = "Čabarkapa, Andrea and Borozan, Sunčica and Živković, Lada and Milanović-Čabarkapa, Mirjana and Stojanović, Srđan and Bajić, Vladan and Spremo-Potparević, Biljana",
year = "2015",
abstract = "The aim of this study was to determine oxidative alterations leading to cellular dysfunctions in Pb-exposed subjects by evaluating damage to all major classes of biomolecules in the cell, lipid peroxidation, protein and DNA damage and determine relationships between parameters of Pb toxicity and specific biomarkers of oxidative damage.Analysis was conducted of smelter workers with high blood Pb and urine aminolevulinic acid levels and slightly elevated values of coproporphyrin and erythrocyte protoporphyrin IX. Significant decreases of thiol groups and increases in carbonyl groups as protein degradation end products, and of nitrite were detected. Elevated rates of lipid peroxidation and rises in the activities of the antioxidant enzymes Cu-Zn superoxide dismutase and catalase were also observed. Both enzymes showed positive correlations with the blood lead levels and urine coproporphyrin, while thiol groups correlated negatively with the same indices. The genotoxic potential of lead was manifested through an increased number of DNA-damaged cells. Increased activities of serum lactate dehydrogenase isoenzymes indicated cellular damage in the lungs, kidneys, and liver. These lead-induced impairments should be taken into consideration in the assessment of Pb-related health hazards.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Toxicology",
title = "Implications of oxidative stress in occupational exposure to lead on a cellular level",
volume = "97",
number = "6",
pages = "799-813",
doi = "10.1080/02772248.2015.1060973"
}

Čabarkapa, A., Borozan, S., Živković, L., Milanović-Čabarkapa, M., Stojanović, S., Bajić, V.,& Spremo-Potparević, B.. (2015). Implications of oxidative stress in occupational exposure to lead on a cellular level. in Toxicology
Taylor & Francis Ltd, Abingdon., 97(6), 799-813.
https://doi.org/10.1080/02772248.2015.1060973

Čabarkapa A, Borozan S, Živković L, Milanović-Čabarkapa M, Stojanović S, Bajić V, Spremo-Potparević B. Implications of oxidative stress in occupational exposure to lead on a cellular level. in Toxicology. 2015;97(6):799-813.
doi:10.1080/02772248.2015.1060973 .

Čabarkapa, Andrea, Borozan, Sunčica, Živković, Lada, Milanović-Čabarkapa, Mirjana, Stojanović, Srđan, Bajić, Vladan, Spremo-Potparević, Biljana, "Implications of oxidative stress in occupational exposure to lead on a cellular level" in Toxicology, 97, no. 6 (2015):799-813,
https://doi.org/10.1080/02772248.2015.1060973 . .

TY  - JOUR
AU  - Topalović, Dijana
AU  - Živković, Lada
AU  - Đelić, Ninoslav
AU  - Bajić, Vladan
AU  - Pirković, Andrea
AU  - Jović, Slavoljub
AU  - Spremo-Potparević, Biljana
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2484
AB  - Hormones are cellular products involved in the regulation of a large number of processes in living systems, and which by their actions affect the growth, function and metabo­lism of cells. Considering that hormones are compounds normally present in the organism, it is important to determine if they can, under certain circumstances, lead to genetic changes in the hereditary material. Numerous experimental studies in vitro and in vivo in different systems, from bacteria to mammals, dealt with the mutagenic and genotoxic effects of hormones. This work presents an overview of the research on genotoxic effects of non­steroidal hormones, although possible changes of genetic material under their influence have not still been known enough, and moreover, investigations on their genotoxic influ­ence have given conflicting results. The study results show that mechanisms of genotoxic effect of nonsteroidal hormones are manifested through the increase of oxidative stress by arising reactive oxygen species. A common mechanism of ROS occurence in thyroid hormones and catecholamines is through metabolic oxidation of their phenolic groups. Mani­festation of insulin genotoxic effect is based on production of ROS by activation of NADPH isophorms, while testing oxytocin showed absence of genotoxic effect. Considering that the investigations on genotoxicity of nonsteroidal hormones demonstrated both positive and negative results, the explanation of this discordance involve limitations of test systems themselves, different cell types or biological species used in the experiments, different lev­el of reactivity in vitro and in vivo, as well as possible variations in a tissue-specific expres­sion. Integrated, the provided data contribute to better understanding of genotoxic effect of nonsteroidal hormones and point out to the role and mode of action of these hormones in the process of occurring of effects caused by oxidative stress.
AB  - Hormoni su ćelijski proizvodi uključeni u regulaciju velikog broja procesa u živim sistemima koji svojim dejstvom utiču na rast, funkciju ili metabolizam ćelija. Obzirom da su hormoni jedinjenja koja su uobičajeno prisutna u organizmu, značajno je utvrditi da li oni mogu pod izvesnim okolnostima dovesti do genetičkih promena na naslednom materijalu. Eksperimentalna ispitivanja u in vitro i in vivo uslovima u različitim sistemima, od bakterija do sisara, bavila su se istraživanjem mutagenih i genotoksičnih efekata hormona. U ovom radu je dat pregled istraživanja genotoksičnih efekata nesteroidnih hormona, pošto još uvek nisu dovoljno poznate moguće promene naslednog materijala pod njihovim uticajem, a i ispitivanja njihovog genotoksičnog dejstva su dala oprečne rezultate. Rezultati studija pokazuju da se mehanizmi genotoksičnog dejstva nesteroidnih hormona ispoljavaju kroz povećanje oksidativnog stresa nastankom reaktivnih vrsta kiseonika (reactive oxygen species - ROS). Uobičajeni mehanizam nastanka ROS kod tireoidnih hormona i kateholamina je putem metaboličke oksidacije njihovih fenolnih grupa. Ispoljavanje genotoksičnog efekta insulina se zasniva na produkciji ROS putem aktivacije NADPH izoformi, dok je ispitivanje oksitocina pokazalo odsustvo genotoksičnog efekta. Uzimajući u obzir da su ispitivanja genotoksičnosti nesteroidnih hormona pokazala i pozitivne i negativne rezultate, objašnjenja ovog neslaganja obuhvataju ograničenja samih test sistema, različite tipove ćelije ili bioloških vrsta upotrebljenih u eksperimentima, različiti nivo reaktivnosti u in vitro i in vivo uslovima, kao i moguće razlike u tkivno specifičnoj ekspresiji. Objedinjeni, navedeni podaci doprinose boljem razumevanju genotoksičnih efekata nesteroidnih hormona i ukazuju na ulogu i načine delovanja ovih hormona u procesu nastanka efekata izazvanih oksidativnim stresom.
PB  - Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd
T2  - Veterinarski glasnik
T1  - Genotoxic potential of nonsteroidal hormones
T1  - Genotoksični potencijal nesteroidnih hormona
VL  - 69
IS  - 3-4
SP  - 245
EP  - 257
DO  - 10.2298/VETGL1504245T
ER  - 

@article{
author = "Topalović, Dijana and Živković, Lada and Đelić, Ninoslav and Bajić, Vladan and Pirković, Andrea and Jović, Slavoljub and Spremo-Potparević, Biljana",
year = "2015",
abstract = "Hormones are cellular products involved in the regulation of a large number of processes in living systems, and which by their actions affect the growth, function and metabo­lism of cells. Considering that hormones are compounds normally present in the organism, it is important to determine if they can, under certain circumstances, lead to genetic changes in the hereditary material. Numerous experimental studies in vitro and in vivo in different systems, from bacteria to mammals, dealt with the mutagenic and genotoxic effects of hormones. This work presents an overview of the research on genotoxic effects of non­steroidal hormones, although possible changes of genetic material under their influence have not still been known enough, and moreover, investigations on their genotoxic influ­ence have given conflicting results. The study results show that mechanisms of genotoxic effect of nonsteroidal hormones are manifested through the increase of oxidative stress by arising reactive oxygen species. A common mechanism of ROS occurence in thyroid hormones and catecholamines is through metabolic oxidation of their phenolic groups. Mani­festation of insulin genotoxic effect is based on production of ROS by activation of NADPH isophorms, while testing oxytocin showed absence of genotoxic effect. Considering that the investigations on genotoxicity of nonsteroidal hormones demonstrated both positive and negative results, the explanation of this discordance involve limitations of test systems themselves, different cell types or biological species used in the experiments, different lev­el of reactivity in vitro and in vivo, as well as possible variations in a tissue-specific expres­sion. Integrated, the provided data contribute to better understanding of genotoxic effect of nonsteroidal hormones and point out to the role and mode of action of these hormones in the process of occurring of effects caused by oxidative stress., Hormoni su ćelijski proizvodi uključeni u regulaciju velikog broja procesa u živim sistemima koji svojim dejstvom utiču na rast, funkciju ili metabolizam ćelija. Obzirom da su hormoni jedinjenja koja su uobičajeno prisutna u organizmu, značajno je utvrditi da li oni mogu pod izvesnim okolnostima dovesti do genetičkih promena na naslednom materijalu. Eksperimentalna ispitivanja u in vitro i in vivo uslovima u različitim sistemima, od bakterija do sisara, bavila su se istraživanjem mutagenih i genotoksičnih efekata hormona. U ovom radu je dat pregled istraživanja genotoksičnih efekata nesteroidnih hormona, pošto još uvek nisu dovoljno poznate moguće promene naslednog materijala pod njihovim uticajem, a i ispitivanja njihovog genotoksičnog dejstva su dala oprečne rezultate. Rezultati studija pokazuju da se mehanizmi genotoksičnog dejstva nesteroidnih hormona ispoljavaju kroz povećanje oksidativnog stresa nastankom reaktivnih vrsta kiseonika (reactive oxygen species - ROS). Uobičajeni mehanizam nastanka ROS kod tireoidnih hormona i kateholamina je putem metaboličke oksidacije njihovih fenolnih grupa. Ispoljavanje genotoksičnog efekta insulina se zasniva na produkciji ROS putem aktivacije NADPH izoformi, dok je ispitivanje oksitocina pokazalo odsustvo genotoksičnog efekta. Uzimajući u obzir da su ispitivanja genotoksičnosti nesteroidnih hormona pokazala i pozitivne i negativne rezultate, objašnjenja ovog neslaganja obuhvataju ograničenja samih test sistema, različite tipove ćelije ili bioloških vrsta upotrebljenih u eksperimentima, različiti nivo reaktivnosti u in vitro i in vivo uslovima, kao i moguće razlike u tkivno specifičnoj ekspresiji. Objedinjeni, navedeni podaci doprinose boljem razumevanju genotoksičnih efekata nesteroidnih hormona i ukazuju na ulogu i načine delovanja ovih hormona u procesu nastanka efekata izazvanih oksidativnim stresom.",
publisher = "Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd",
journal = "Veterinarski glasnik",
title = "Genotoxic potential of nonsteroidal hormones, Genotoksični potencijal nesteroidnih hormona",
volume = "69",
number = "3-4",
pages = "245-257",
doi = "10.2298/VETGL1504245T"
}

Topalović, D., Živković, L., Đelić, N., Bajić, V., Pirković, A., Jović, S.,& Spremo-Potparević, B.. (2015). Genotoxic potential of nonsteroidal hormones. in Veterinarski glasnik
Univerzitet u Beogradu - Fakultet veterinarske medicine, Beograd., 69(3-4), 245-257.
https://doi.org/10.2298/VETGL1504245T

Topalović D, Živković L, Đelić N, Bajić V, Pirković A, Jović S, Spremo-Potparević B. Genotoxic potential of nonsteroidal hormones. in Veterinarski glasnik. 2015;69(3-4):245-257.
doi:10.2298/VETGL1504245T .

Topalović, Dijana, Živković, Lada, Đelić, Ninoslav, Bajić, Vladan, Pirković, Andrea, Jović, Slavoljub, Spremo-Potparević, Biljana, "Genotoxic potential of nonsteroidal hormones" in Veterinarski glasnik, 69, no. 3-4 (2015):245-257,
https://doi.org/10.2298/VETGL1504245T . .

TY  - JOUR
AU  - Čabarkapa, Andrea
AU  - Borozan, Sunčica
AU  - Živković, Lada
AU  - Milanović-Čabarkapa, Mirjana
AU  - Stojanović, Srđan
AU  - Bajić, Vladan
AU  - Spremo-Potparević, Biljana
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2440
AB  - The aim of this study was to determine oxidative alterations leading to cellular dysfunctions in Pb-exposed subjects by evaluating damage to all major classes of biomolecules in the cell, lipid peroxidation, protein and DNA damage and determine relationships between parameters of Pb toxicity and specific biomarkers of oxidative damage.Analysis was conducted of smelter workers with high blood Pb and urine aminolevulinic acid levels and slightly elevated values of coproporphyrin and erythrocyte protoporphyrin IX. Significant decreases of thiol groups and increases in carbonyl groups as protein degradation end products, and of nitrite were detected. Elevated rates of lipid peroxidation and rises in the activities of the antioxidant enzymes Cu-Zn superoxide dismutase and catalase were also observed. Both enzymes showed positive correlations with the blood lead levels and urine coproporphyrin, while thiol groups correlated negatively with the same indices. The genotoxic potential of lead was manifested through an increased number of DNA-damaged cells. Increased activities of serum lactate dehydrogenase isoenzymes indicated cellular damage in the lungs, kidneys, and liver. These lead-induced impairments should be taken into consideration in the assessment of Pb-related health hazards.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Toxicology
T1  - Implications of oxidative stress in occupational exposure to lead on a cellular level
VL  - 97
IS  - 6
SP  - 799
EP  - 813
DO  - 10.1080/02772248.2015.1060973
ER  - 

@article{
author = "Čabarkapa, Andrea and Borozan, Sunčica and Živković, Lada and Milanović-Čabarkapa, Mirjana and Stojanović, Srđan and Bajić, Vladan and Spremo-Potparević, Biljana",
year = "2015",
abstract = "The aim of this study was to determine oxidative alterations leading to cellular dysfunctions in Pb-exposed subjects by evaluating damage to all major classes of biomolecules in the cell, lipid peroxidation, protein and DNA damage and determine relationships between parameters of Pb toxicity and specific biomarkers of oxidative damage.Analysis was conducted of smelter workers with high blood Pb and urine aminolevulinic acid levels and slightly elevated values of coproporphyrin and erythrocyte protoporphyrin IX. Significant decreases of thiol groups and increases in carbonyl groups as protein degradation end products, and of nitrite were detected. Elevated rates of lipid peroxidation and rises in the activities of the antioxidant enzymes Cu-Zn superoxide dismutase and catalase were also observed. Both enzymes showed positive correlations with the blood lead levels and urine coproporphyrin, while thiol groups correlated negatively with the same indices. The genotoxic potential of lead was manifested through an increased number of DNA-damaged cells. Increased activities of serum lactate dehydrogenase isoenzymes indicated cellular damage in the lungs, kidneys, and liver. These lead-induced impairments should be taken into consideration in the assessment of Pb-related health hazards.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Toxicology",
title = "Implications of oxidative stress in occupational exposure to lead on a cellular level",
volume = "97",
number = "6",
pages = "799-813",
doi = "10.1080/02772248.2015.1060973"
}

Čabarkapa, A., Borozan, S., Živković, L., Milanović-Čabarkapa, M., Stojanović, S., Bajić, V.,& Spremo-Potparević, B.. (2015). Implications of oxidative stress in occupational exposure to lead on a cellular level. in Toxicology
Taylor & Francis Ltd, Abingdon., 97(6), 799-813.
https://doi.org/10.1080/02772248.2015.1060973

Čabarkapa A, Borozan S, Živković L, Milanović-Čabarkapa M, Stojanović S, Bajić V, Spremo-Potparević B. Implications of oxidative stress in occupational exposure to lead on a cellular level. in Toxicology. 2015;97(6):799-813.
doi:10.1080/02772248.2015.1060973 .

Čabarkapa, Andrea, Borozan, Sunčica, Živković, Lada, Milanović-Čabarkapa, Mirjana, Stojanović, Srđan, Bajić, Vladan, Spremo-Potparević, Biljana, "Implications of oxidative stress in occupational exposure to lead on a cellular level" in Toxicology, 97, no. 6 (2015):799-813,
https://doi.org/10.1080/02772248.2015.1060973 . .

TY  - JOUR
AU  - Topalović, Dijana
AU  - Živković, Lada
AU  - Pirković, Andrea
AU  - Đelić, Ninoslav
AU  - Bajić, Vladan
AU  - Dekanski, Dragana
AU  - Potparević, Biljana
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2384
AB  - The thyroid hormones change the rate of basal metabolism, modulating the consumption of oxygen and causing production of reactive oxygen species, which leads to the development of oxidative stress and DNA strand breaks. Olive (Olea europaea L.) leaf contains many potentially bioactive compounds, making it one of the most potent natural antioxidants. The objective of this study was to evaluate the genotoxicity of L-thyroxine and to investigate antioxidative and antigenotoxic potential of the standardized oleuropein-rich dry olive leaf extract (DOLE) against hydrogen peroxide and L-thyroxine-induced DNA damage in human peripheral blood leukocytes by using the comet assay. Various concentrations of the extract were tested with both DNA damage inducers, under two different experimental conditions, pretreatment and posttreatment. Results indicate that L-thyroxine exhibited genotoxic effect and that DOLE displayed protective effect against thyroxine-induced genotoxicity. The number of cells with DNA damage, was significantly reduced, in both pretreated and posttreated samples (P  lt  0.05). Comparing the beneficial effect of all tested concentrations of DOLE, in both experimental protocols, it appears that extract was more effective in reducing DNA damage in the pretreatment, exhibiting protective role against L-thyroxine effect. This feature of DOLE can be explained by its capacity to act as potent free radical scavenger.
PB  - Hindawi Ltd, London
T2  - Oxidative Medicine and Cellular Longevity
T1  - Dry Olive Leaf Extract Counteracts L-Thyroxine-Induced Genotoxicity in Human Peripheral Blood Leukocytes In Vitro
DO  - 10.1155/2015/762192
ER  - 

@article{
author = "Topalović, Dijana and Živković, Lada and Pirković, Andrea and Đelić, Ninoslav and Bajić, Vladan and Dekanski, Dragana and Potparević, Biljana",
year = "2015",
abstract = "The thyroid hormones change the rate of basal metabolism, modulating the consumption of oxygen and causing production of reactive oxygen species, which leads to the development of oxidative stress and DNA strand breaks. Olive (Olea europaea L.) leaf contains many potentially bioactive compounds, making it one of the most potent natural antioxidants. The objective of this study was to evaluate the genotoxicity of L-thyroxine and to investigate antioxidative and antigenotoxic potential of the standardized oleuropein-rich dry olive leaf extract (DOLE) against hydrogen peroxide and L-thyroxine-induced DNA damage in human peripheral blood leukocytes by using the comet assay. Various concentrations of the extract were tested with both DNA damage inducers, under two different experimental conditions, pretreatment and posttreatment. Results indicate that L-thyroxine exhibited genotoxic effect and that DOLE displayed protective effect against thyroxine-induced genotoxicity. The number of cells with DNA damage, was significantly reduced, in both pretreated and posttreated samples (P  lt  0.05). Comparing the beneficial effect of all tested concentrations of DOLE, in both experimental protocols, it appears that extract was more effective in reducing DNA damage in the pretreatment, exhibiting protective role against L-thyroxine effect. This feature of DOLE can be explained by its capacity to act as potent free radical scavenger.",
publisher = "Hindawi Ltd, London",
journal = "Oxidative Medicine and Cellular Longevity",
title = "Dry Olive Leaf Extract Counteracts L-Thyroxine-Induced Genotoxicity in Human Peripheral Blood Leukocytes In Vitro",
doi = "10.1155/2015/762192"
}

Topalović, D., Živković, L., Pirković, A., Đelić, N., Bajić, V., Dekanski, D.,& Potparević, B.. (2015). Dry Olive Leaf Extract Counteracts L-Thyroxine-Induced Genotoxicity in Human Peripheral Blood Leukocytes In Vitro. in Oxidative Medicine and Cellular Longevity
Hindawi Ltd, London..
https://doi.org/10.1155/2015/762192

Topalović D, Živković L, Pirković A, Đelić N, Bajić V, Dekanski D, Potparević B. Dry Olive Leaf Extract Counteracts L-Thyroxine-Induced Genotoxicity in Human Peripheral Blood Leukocytes In Vitro. in Oxidative Medicine and Cellular Longevity. 2015;.
doi:10.1155/2015/762192 .

Topalović, Dijana, Živković, Lada, Pirković, Andrea, Đelić, Ninoslav, Bajić, Vladan, Dekanski, Dragana, Potparević, Biljana, "Dry Olive Leaf Extract Counteracts L-Thyroxine-Induced Genotoxicity in Human Peripheral Blood Leukocytes In Vitro" in Oxidative Medicine and Cellular Longevity (2015),
https://doi.org/10.1155/2015/762192 . .

TY  - JOUR
AU  - Pirković, Andrea
AU  - Borozan, Sunčica
AU  - Živković, Lada
AU  - Stojanović, Srđan
AU  - Milanović-Čabarkapa, Mirjana
AU  - Bajić, Vladan
AU  - Potparević, Biljana
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2359
AB  - Lead induced oxidative cellular damage and long-term persistence of associated adverse effects increases risk of late-onset diseases. CaNa(2)EDTA chelation is known to remove contaminating metals and to reduce free radical production. The objective was to investigate the impact of chelation therapy on modulation of lead induced cellular damage, restoration of altered enzyme activities and lipid homeostasis in peripheral blood of workers exposed to lead, by comparing the selected biomarkers obtained prior and after five-day CaNa(2)EDTA chelation intervention. The group of smelting factory workers diagnosed with lead intoxication and current lead exposure 5.8 +/- 1.2 years were administered five-day CaNa(2)EDTA chelation. Elevated baseline activity of antioxidant enzymes Cu, Zn-SOD and CAT as well as depleted thiols and increased protein degradation products-carbonyl groups and nitrites, pointing to Pb induced oxidative damage, were restored toward normal values following the treatment. Lead showed inhibitor potency on both RBC AChE and BChE in exposed workers, and chelation re-established the activity of BChE, while RBC AChE remained unaffected. Also, genotoxic effect of lead detected in peripheral blood lymphocytes was significantly decreased after therapy, exhibiting 18.9% DNA damage reduction. Administration of chelation reversed the depressed activity of serum PON 1 and significantly decreased lipid peroxidation detected by the post-chelation reduction of MDA levels. Lactate dehydrogenase LDF1-5 isoenzymes levels showed evident but no significant trend of restoring toward normal control values following chelation. CaNa(2)EDTA chelation ameliorates the alterations linked with Pb mediated oxidative stress, indicating possible benefits in reducing health risks associated with increased oxidative damage in lead exposed populations.
PB  - Elsevier Ireland Ltd, Clare
T2  - Chemico-Biological Interactions
T1  - CaNa(2)EDTA chelation attenuates cell damage in workers exposed to lead-a pilot study
VL  - 242
SP  - 171
EP  - 178
DO  - 10.1016/j.cbi.2015.10.002
ER  - 

@article{
author = "Pirković, Andrea and Borozan, Sunčica and Živković, Lada and Stojanović, Srđan and Milanović-Čabarkapa, Mirjana and Bajić, Vladan and Potparević, Biljana",
year = "2015",
abstract = "Lead induced oxidative cellular damage and long-term persistence of associated adverse effects increases risk of late-onset diseases. CaNa(2)EDTA chelation is known to remove contaminating metals and to reduce free radical production. The objective was to investigate the impact of chelation therapy on modulation of lead induced cellular damage, restoration of altered enzyme activities and lipid homeostasis in peripheral blood of workers exposed to lead, by comparing the selected biomarkers obtained prior and after five-day CaNa(2)EDTA chelation intervention. The group of smelting factory workers diagnosed with lead intoxication and current lead exposure 5.8 +/- 1.2 years were administered five-day CaNa(2)EDTA chelation. Elevated baseline activity of antioxidant enzymes Cu, Zn-SOD and CAT as well as depleted thiols and increased protein degradation products-carbonyl groups and nitrites, pointing to Pb induced oxidative damage, were restored toward normal values following the treatment. Lead showed inhibitor potency on both RBC AChE and BChE in exposed workers, and chelation re-established the activity of BChE, while RBC AChE remained unaffected. Also, genotoxic effect of lead detected in peripheral blood lymphocytes was significantly decreased after therapy, exhibiting 18.9% DNA damage reduction. Administration of chelation reversed the depressed activity of serum PON 1 and significantly decreased lipid peroxidation detected by the post-chelation reduction of MDA levels. Lactate dehydrogenase LDF1-5 isoenzymes levels showed evident but no significant trend of restoring toward normal control values following chelation. CaNa(2)EDTA chelation ameliorates the alterations linked with Pb mediated oxidative stress, indicating possible benefits in reducing health risks associated with increased oxidative damage in lead exposed populations.",
publisher = "Elsevier Ireland Ltd, Clare",
journal = "Chemico-Biological Interactions",
title = "CaNa(2)EDTA chelation attenuates cell damage in workers exposed to lead-a pilot study",
volume = "242",
pages = "171-178",
doi = "10.1016/j.cbi.2015.10.002"
}

Pirković, A., Borozan, S., Živković, L., Stojanović, S., Milanović-Čabarkapa, M., Bajić, V.,& Potparević, B.. (2015). CaNa(2)EDTA chelation attenuates cell damage in workers exposed to lead-a pilot study. in Chemico-Biological Interactions
Elsevier Ireland Ltd, Clare., 242, 171-178.
https://doi.org/10.1016/j.cbi.2015.10.002

Pirković A, Borozan S, Živković L, Stojanović S, Milanović-Čabarkapa M, Bajić V, Potparević B. CaNa(2)EDTA chelation attenuates cell damage in workers exposed to lead-a pilot study. in Chemico-Biological Interactions. 2015;242:171-178.
doi:10.1016/j.cbi.2015.10.002 .

Pirković, Andrea, Borozan, Sunčica, Živković, Lada, Stojanović, Srđan, Milanović-Čabarkapa, Mirjana, Bajić, Vladan, Potparević, Biljana, "CaNa(2)EDTA chelation attenuates cell damage in workers exposed to lead-a pilot study" in Chemico-Biological Interactions, 242 (2015):171-178,
https://doi.org/10.1016/j.cbi.2015.10.002 . .

TY  - JOUR
AU  - Knežević, A
AU  - Živković, Lada
AU  - Stajić, Mirjana
AU  - Vukojević, Jelena
AU  - Milovanović, I
AU  - Potparević, Biljana
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2495
AB  - Trametes species have been used for thousands of years in traditional and conventional medicine for the treatment of various types of diseases. The goal was to evaluate possible antigenotoxic effects of mycelium and basidiocarp extracts of selected Trametes species and to assess dependence on their antioxidant potential. Trametes versicolor, T. hirsuta, and T. gibbosa were the species studied. Antigenotoxic potentials of extracts were assessed on human peripheral white blood cells with basidiocarp and mycelium extracts of the species. The alkaline comet test was used for detection of DNA strand breaks and alkali-labile sites, as well as the extent of DNA migration. DPPH assay was used to estimate antioxidative properties of extracts. Fruiting body extracts of T. versicolor and T. gibbosa as well as T. hirsuta extracts, except that at 20.0 mg/mL, were not genotoxic agents. T. versicolor extract had at 5.0 mg/mL the greatest antigenotoxic effect in both pre-and posttreatment of leukocytes. The mycelium extracts of the three species had no genotoxic activity and significant antigenotoxic effect against H lt inf>2 lt /inf>O lt inf>2 lt /inf>-induced DNA damage, both in pre-and posttreatment. The results suggest that extracts of these three species could be considered as strong antigenotoxic agents able to stimulate genoprotective response of cells.
PB  - Hindawi Publishing Corporation
T2  - Scientific World Journal
T1  - Antigenotoxic Effect of Trametes spp. Extracts against DNA Damage on Human Peripheral White Blood Cells
VL  - 2015
DO  - 10.1155/2015/146378
ER  - 

@article{
author = "Knežević, A and Živković, Lada and Stajić, Mirjana and Vukojević, Jelena and Milovanović, I and Potparević, Biljana",
year = "2015",
abstract = "Trametes species have been used for thousands of years in traditional and conventional medicine for the treatment of various types of diseases. The goal was to evaluate possible antigenotoxic effects of mycelium and basidiocarp extracts of selected Trametes species and to assess dependence on their antioxidant potential. Trametes versicolor, T. hirsuta, and T. gibbosa were the species studied. Antigenotoxic potentials of extracts were assessed on human peripheral white blood cells with basidiocarp and mycelium extracts of the species. The alkaline comet test was used for detection of DNA strand breaks and alkali-labile sites, as well as the extent of DNA migration. DPPH assay was used to estimate antioxidative properties of extracts. Fruiting body extracts of T. versicolor and T. gibbosa as well as T. hirsuta extracts, except that at 20.0 mg/mL, were not genotoxic agents. T. versicolor extract had at 5.0 mg/mL the greatest antigenotoxic effect in both pre-and posttreatment of leukocytes. The mycelium extracts of the three species had no genotoxic activity and significant antigenotoxic effect against H lt inf>2 lt /inf>O lt inf>2 lt /inf>-induced DNA damage, both in pre-and posttreatment. The results suggest that extracts of these three species could be considered as strong antigenotoxic agents able to stimulate genoprotective response of cells.",
publisher = "Hindawi Publishing Corporation",
journal = "Scientific World Journal",
title = "Antigenotoxic Effect of Trametes spp. Extracts against DNA Damage on Human Peripheral White Blood Cells",
volume = "2015",
doi = "10.1155/2015/146378"
}

Knežević, A., Živković, L., Stajić, M., Vukojević, J., Milovanović, I.,& Potparević, B.. (2015). Antigenotoxic Effect of Trametes spp. Extracts against DNA Damage on Human Peripheral White Blood Cells. in Scientific World Journal
Hindawi Publishing Corporation., 2015.
https://doi.org/10.1155/2015/146378

Knežević A, Živković L, Stajić M, Vukojević J, Milovanović I, Potparević B. Antigenotoxic Effect of Trametes spp. Extracts against DNA Damage on Human Peripheral White Blood Cells. in Scientific World Journal. 2015;2015.
doi:10.1155/2015/146378 .

Knežević, A, Živković, Lada, Stajić, Mirjana, Vukojević, Jelena, Milovanović, I, Potparević, Biljana, "Antigenotoxic Effect of Trametes spp. Extracts against DNA Damage on Human Peripheral White Blood Cells" in Scientific World Journal, 2015 (2015),
https://doi.org/10.1155/2015/146378 . .

TY  - JOUR
AU  - Bajić, Vladan
AU  - Potparević, Biljana
AU  - Živković, Lada
AU  - Isenović, Esma
AU  - Arendt, Thomas
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2430
AB  - Neurons are postmitotic cells that are in permanent cell cycle arrest. However, components of the cell cycle machinery that are expressed in Alzheimer's disease (AD) neurons are showing features of a cycling cell and those attributed to a postmitotic cell as well. Furthermore, the unique physiological operations taking place in neurons, ascribed to "core cell cycle regulators" are also key regulators in cell division. Functions of these cell cycle regulators include neuronal migration, axonal elongation, axon pruning, dendrite morphogenesis and synaptic maturation and plasticity. In this review, we focus on cohesion and cohesion related proteins in reference to their neuronal functions and how impaired centromere/cohesion dynamics may connect cell cycle dysfunction to aneuploidy in AD.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Neuroscience and Biobehavioral Reviews
T1  - Cohesion and the aneuploid phenotype in Alzheimer's disease: A tale of genome instability
VL  - 55
SP  - 365
EP  - 374
DO  - 10.1016/j.neubiorev.2015.05.010
ER  - 

@article{
author = "Bajić, Vladan and Potparević, Biljana and Živković, Lada and Isenović, Esma and Arendt, Thomas",
year = "2015",
abstract = "Neurons are postmitotic cells that are in permanent cell cycle arrest. However, components of the cell cycle machinery that are expressed in Alzheimer's disease (AD) neurons are showing features of a cycling cell and those attributed to a postmitotic cell as well. Furthermore, the unique physiological operations taking place in neurons, ascribed to "core cell cycle regulators" are also key regulators in cell division. Functions of these cell cycle regulators include neuronal migration, axonal elongation, axon pruning, dendrite morphogenesis and synaptic maturation and plasticity. In this review, we focus on cohesion and cohesion related proteins in reference to their neuronal functions and how impaired centromere/cohesion dynamics may connect cell cycle dysfunction to aneuploidy in AD.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Neuroscience and Biobehavioral Reviews",
title = "Cohesion and the aneuploid phenotype in Alzheimer's disease: A tale of genome instability",
volume = "55",
pages = "365-374",
doi = "10.1016/j.neubiorev.2015.05.010"
}

Bajić, V., Potparević, B., Živković, L., Isenović, E.,& Arendt, T.. (2015). Cohesion and the aneuploid phenotype in Alzheimer's disease: A tale of genome instability. in Neuroscience and Biobehavioral Reviews
Pergamon-Elsevier Science Ltd, Oxford., 55, 365-374.
https://doi.org/10.1016/j.neubiorev.2015.05.010

Bajić V, Potparević B, Živković L, Isenović E, Arendt T. Cohesion and the aneuploid phenotype in Alzheimer's disease: A tale of genome instability. in Neuroscience and Biobehavioral Reviews. 2015;55:365-374.
doi:10.1016/j.neubiorev.2015.05.010 .

Bajić, Vladan, Potparević, Biljana, Živković, Lada, Isenović, Esma, Arendt, Thomas, "Cohesion and the aneuploid phenotype in Alzheimer's disease: A tale of genome instability" in Neuroscience and Biobehavioral Reviews, 55 (2015):365-374,
https://doi.org/10.1016/j.neubiorev.2015.05.010 . .

TY  - JOUR
AU  - Bajić, Vladan
AU  - Mandusić, Vesna
AU  - Stefanova, Elka
AU  - Bozović, Ana
AU  - Davidović, Radoslav
AU  - Živković, Lada
AU  - Pirković, Andrea
AU  - Potparević, Biljana
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2386
AB  - X-chromosome instability has been a long established feature in Alzheimer's disease ( AD). Premature centromere division and aneuploidy of the X-chromosome has been found in peripheral blood lymphocytes and neuronal tissue in female AD patients. Interestingly, only one chromosome of the X pair has been affected. These results raised a question, "Is the X-chromosome inactivation pattern altered in peripheral blood lymphocytes ofwomen affected by AD?" To address this question, we analyzed the methylation status of androgen receptor promoter which may show us any deviation from the 50 : 50% X inactivation status in peripheral blood lymphocytes ofwomen with AD. Our results showed skewed inactivation patterns (>90%). These findings suggest that an epigenetic alteration on the inactivation centers of the X-chromosome (or skewing) relates not only to aging, by might be a novel property that could account for the higher incidence of AD in women.
PB  - Ios Press, Amsterdam
T2  - Journal of Alzheimers Disease
T1  - Skewed X-Chromosome Inactivation in Women Affected by Alzheimer's Disease
VL  - 43
IS  - 4
SP  - 1251
EP  - 1259
DO  - 10.3233/JAD-141674
ER  - 

@article{
author = "Bajić, Vladan and Mandusić, Vesna and Stefanova, Elka and Bozović, Ana and Davidović, Radoslav and Živković, Lada and Pirković, Andrea and Potparević, Biljana",
year = "2015",
abstract = "X-chromosome instability has been a long established feature in Alzheimer's disease ( AD). Premature centromere division and aneuploidy of the X-chromosome has been found in peripheral blood lymphocytes and neuronal tissue in female AD patients. Interestingly, only one chromosome of the X pair has been affected. These results raised a question, "Is the X-chromosome inactivation pattern altered in peripheral blood lymphocytes ofwomen affected by AD?" To address this question, we analyzed the methylation status of androgen receptor promoter which may show us any deviation from the 50 : 50% X inactivation status in peripheral blood lymphocytes ofwomen with AD. Our results showed skewed inactivation patterns (>90%). These findings suggest that an epigenetic alteration on the inactivation centers of the X-chromosome (or skewing) relates not only to aging, by might be a novel property that could account for the higher incidence of AD in women.",
publisher = "Ios Press, Amsterdam",
journal = "Journal of Alzheimers Disease",
title = "Skewed X-Chromosome Inactivation in Women Affected by Alzheimer's Disease",
volume = "43",
number = "4",
pages = "1251-1259",
doi = "10.3233/JAD-141674"
}

Bajić, V., Mandusić, V., Stefanova, E., Bozović, A., Davidović, R., Živković, L., Pirković, A.,& Potparević, B.. (2015). Skewed X-Chromosome Inactivation in Women Affected by Alzheimer's Disease. in Journal of Alzheimers Disease
Ios Press, Amsterdam., 43(4), 1251-1259.
https://doi.org/10.3233/JAD-141674

Bajić V, Mandusić V, Stefanova E, Bozović A, Davidović R, Živković L, Pirković A, Potparević B. Skewed X-Chromosome Inactivation in Women Affected by Alzheimer's Disease. in Journal of Alzheimers Disease. 2015;43(4):1251-1259.
doi:10.3233/JAD-141674 .

Bajić, Vladan, Mandusić, Vesna, Stefanova, Elka, Bozović, Ana, Davidović, Radoslav, Živković, Lada, Pirković, Andrea, Potparević, Biljana, "Skewed X-Chromosome Inactivation in Women Affected by Alzheimer's Disease" in Journal of Alzheimers Disease, 43, no. 4 (2015):1251-1259,
https://doi.org/10.3233/JAD-141674 . .