TY  - JOUR
AU  - Rajkov, Branislava
AU  - Zdravković, Marija
AU  - Ninić, Ana
AU  - Brajković, Milica
AU  - Klašnja, Slobodan
AU  - Gardijan, Vera
AU  - Memon, Lidija
AU  - Munjas, Jelena
AU  - Mihajlović, Marija
AU  - Spasojević-Kalimanovska, Vesna
AU  - Radosavljević, Vojislav
AU  - Sopić, Miron
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4551
AB  - Purpose: Obstructive sleep apnea (OSA) is characterised by increased systemic inflammation, and is often accompanied with type 2 diabetes mellitus (T2DM) and cardiovascular disease. The aim of this investigation was to evaluate gene expression of resistin, its receptor CAP1 and CD36 as the indicators of the inflammatory changes in PBMCs in relation to the severity of OSA, and the presence of type 2 diabetes mellitus (T2DM) in OSA. Methods: Severity of OSA was defined by the apnea/hypopnea index (AHI): AHI < 30: mild to moderate OSA (MM-OSA), AHI ≥ 30: severe OSA (S-OSA). Presence of T2DM was captured: OSA with T2DM (OSA + T2DM), OSA without T2DM (OSA-T2DM). PBMC resistin, CAP1, and CD36 mRNA were determined by real-time PCR. Results: Resistin mRNA was significantly upregulated in S-OSA (N = 54) compared to the MM-OSA (N = 52, P = 0.043); CAP1 and CD36 mRNA levels did not differ between the groups (P = 0.302; P = 0.166, respectively). Resistin mRNA was significantly upregulated in OSA + T2DM (N = 29) compared to the OSA-T2DM (N = 77, P = 0.029); CAP1 and CD36 mRNA levels did not differ between the groups (P = 0.662; P = 0.108, respectively). AHI and T2DM were independent predictors of resistin mRNA above the 75th percentile (OR = 3.717 [1.152–11.991]; OR = 3.261 [1.000–10.630], P = 0.042 respectively). Conclusion: Resistin gene upregulation in S-OSA indicates its possible contribution to increased inflammation in S-OSA and makes it a possible marker of the disease severity. Resistin gene upregulation in OSA + T2DM suggests that a joint effect of these two comorbidities may have a major contribution to increased inflammation and complications that arise from this state.
PB  - Springer Nature
T2  - Sleep and Breathing
T1  - Upregulation of peripheral blood mononuclear cells resistin gene expression in severe obstructive sleep apnea and obstructive sleep apnea with coexisting type 2 diabetes mellitus
DO  - 10.1007/s11325-023-02809-0
ER  - 

@article{
author = "Rajkov, Branislava and Zdravković, Marija and Ninić, Ana and Brajković, Milica and Klašnja, Slobodan and Gardijan, Vera and Memon, Lidija and Munjas, Jelena and Mihajlović, Marija and Spasojević-Kalimanovska, Vesna and Radosavljević, Vojislav and Sopić, Miron",
year = "2023",
abstract = "Purpose: Obstructive sleep apnea (OSA) is characterised by increased systemic inflammation, and is often accompanied with type 2 diabetes mellitus (T2DM) and cardiovascular disease. The aim of this investigation was to evaluate gene expression of resistin, its receptor CAP1 and CD36 as the indicators of the inflammatory changes in PBMCs in relation to the severity of OSA, and the presence of type 2 diabetes mellitus (T2DM) in OSA. Methods: Severity of OSA was defined by the apnea/hypopnea index (AHI): AHI < 30: mild to moderate OSA (MM-OSA), AHI ≥ 30: severe OSA (S-OSA). Presence of T2DM was captured: OSA with T2DM (OSA + T2DM), OSA without T2DM (OSA-T2DM). PBMC resistin, CAP1, and CD36 mRNA were determined by real-time PCR. Results: Resistin mRNA was significantly upregulated in S-OSA (N = 54) compared to the MM-OSA (N = 52, P = 0.043); CAP1 and CD36 mRNA levels did not differ between the groups (P = 0.302; P = 0.166, respectively). Resistin mRNA was significantly upregulated in OSA + T2DM (N = 29) compared to the OSA-T2DM (N = 77, P = 0.029); CAP1 and CD36 mRNA levels did not differ between the groups (P = 0.662; P = 0.108, respectively). AHI and T2DM were independent predictors of resistin mRNA above the 75th percentile (OR = 3.717 [1.152–11.991]; OR = 3.261 [1.000–10.630], P = 0.042 respectively). Conclusion: Resistin gene upregulation in S-OSA indicates its possible contribution to increased inflammation in S-OSA and makes it a possible marker of the disease severity. Resistin gene upregulation in OSA + T2DM suggests that a joint effect of these two comorbidities may have a major contribution to increased inflammation and complications that arise from this state.",
publisher = "Springer Nature",
journal = "Sleep and Breathing",
title = "Upregulation of peripheral blood mononuclear cells resistin gene expression in severe obstructive sleep apnea and obstructive sleep apnea with coexisting type 2 diabetes mellitus",
doi = "10.1007/s11325-023-02809-0"
}

Rajkov, B., Zdravković, M., Ninić, A., Brajković, M., Klašnja, S., Gardijan, V., Memon, L., Munjas, J., Mihajlović, M., Spasojević-Kalimanovska, V., Radosavljević, V.,& Sopić, M.. (2023). Upregulation of peripheral blood mononuclear cells resistin gene expression in severe obstructive sleep apnea and obstructive sleep apnea with coexisting type 2 diabetes mellitus. in Sleep and Breathing
Springer Nature..
https://doi.org/10.1007/s11325-023-02809-0

Rajkov B, Zdravković M, Ninić A, Brajković M, Klašnja S, Gardijan V, Memon L, Munjas J, Mihajlović M, Spasojević-Kalimanovska V, Radosavljević V, Sopić M. Upregulation of peripheral blood mononuclear cells resistin gene expression in severe obstructive sleep apnea and obstructive sleep apnea with coexisting type 2 diabetes mellitus. in Sleep and Breathing. 2023;.
doi:10.1007/s11325-023-02809-0 .

Rajkov, Branislava, Zdravković, Marija, Ninić, Ana, Brajković, Milica, Klašnja, Slobodan, Gardijan, Vera, Memon, Lidija, Munjas, Jelena, Mihajlović, Marija, Spasojević-Kalimanovska, Vesna, Radosavljević, Vojislav, Sopić, Miron, "Upregulation of peripheral blood mononuclear cells resistin gene expression in severe obstructive sleep apnea and obstructive sleep apnea with coexisting type 2 diabetes mellitus" in Sleep and Breathing (2023),
https://doi.org/10.1007/s11325-023-02809-0 . .

TY  - JOUR
AU  - Brborić, Jasmina
AU  - Klisić, Aleksandra
AU  - Kotur-Stevuljević, Jelena
AU  - Delogu, Giovanna
AU  - Gjorgieva Ackova, Darinka
AU  - Kostić, Kristina
AU  - Dettori, Maria Antonietta
AU  - Fabbri, Davide
AU  - Carta, Paola
AU  - Saso, Luciano
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4964
AB  - Introduction: Phenols are a large family of natural and synthetic compounds with known antioxidant activity. The aim of this study was to perform in vitro screening of natural and natural-like phenol monomers and their C2-symmetric dimers (hydroxylated biphenyls) in order to identify those representatives whose pharmacophores have the strongest antioxidant and the lowest prooxidant activity. Material and methods: Antioxidative properties of 36 compounds (monomers and their C2-symmetric dimers) were evaluated in vitro. Different (red/ ox) assays were used to measure their total oxidative potential (TOP), their total antioxidative capacity (TAC), the pro-oxidative-antioxidant balance (PAB) and total SH-group content (SHG) in a biologically relevant environment. The Pro-oxidative Score, Antioxidative Score and the Oxy Score were also calculated. Trolox, a water soluble analogue of α-tocopherol, was used as a positive control. Results: In an assay consisting of pooled human serum, 6 of the 36 compounds showed significant antioxidant activity (compounds 6, 7, 12, 13, 26, and 27), whereas 4 showed extremely weak antioxidant activity (compounds 2, 29, 30, and 31). Within the 36 compounds comprising zingerone, dehydrozingerone, aurone, chalcone, and magnolol derivatives, in both monomeric and dimeric forms, the 2 compounds that indicated the highest antioxidant activity were dehydrozingerone derivatives (compounds 6 and 12). Trolox’s activity was found between the strong and weak antioxidant compounds analysed in our study. Conclusions: In this study selected dehydrozingerones were identified as good candidates for in-depth testing of their biological behaviour and for possible precursors for synthesis of novel polyphenolic molecules with potential therapeutic applications.
PB  - Termedia Publishing House
T2  - Archives of Medical Science
T1  - Natural and natural-like polyphenol compounds: in vitro antioxidant activity and potential for therapeutic application
VL  - 19
IS  - 3
SP  - 651
EP  - 671
DO  - 10.5114/aoms/135379
ER  - 

@article{
author = "Brborić, Jasmina and Klisić, Aleksandra and Kotur-Stevuljević, Jelena and Delogu, Giovanna and Gjorgieva Ackova, Darinka and Kostić, Kristina and Dettori, Maria Antonietta and Fabbri, Davide and Carta, Paola and Saso, Luciano",
year = "2023",
abstract = "Introduction: Phenols are a large family of natural and synthetic compounds with known antioxidant activity. The aim of this study was to perform in vitro screening of natural and natural-like phenol monomers and their C2-symmetric dimers (hydroxylated biphenyls) in order to identify those representatives whose pharmacophores have the strongest antioxidant and the lowest prooxidant activity. Material and methods: Antioxidative properties of 36 compounds (monomers and their C2-symmetric dimers) were evaluated in vitro. Different (red/ ox) assays were used to measure their total oxidative potential (TOP), their total antioxidative capacity (TAC), the pro-oxidative-antioxidant balance (PAB) and total SH-group content (SHG) in a biologically relevant environment. The Pro-oxidative Score, Antioxidative Score and the Oxy Score were also calculated. Trolox, a water soluble analogue of α-tocopherol, was used as a positive control. Results: In an assay consisting of pooled human serum, 6 of the 36 compounds showed significant antioxidant activity (compounds 6, 7, 12, 13, 26, and 27), whereas 4 showed extremely weak antioxidant activity (compounds 2, 29, 30, and 31). Within the 36 compounds comprising zingerone, dehydrozingerone, aurone, chalcone, and magnolol derivatives, in both monomeric and dimeric forms, the 2 compounds that indicated the highest antioxidant activity were dehydrozingerone derivatives (compounds 6 and 12). Trolox’s activity was found between the strong and weak antioxidant compounds analysed in our study. Conclusions: In this study selected dehydrozingerones were identified as good candidates for in-depth testing of their biological behaviour and for possible precursors for synthesis of novel polyphenolic molecules with potential therapeutic applications.",
publisher = "Termedia Publishing House",
journal = "Archives of Medical Science",
title = "Natural and natural-like polyphenol compounds: in vitro antioxidant activity and potential for therapeutic application",
volume = "19",
number = "3",
pages = "651-671",
doi = "10.5114/aoms/135379"
}

Brborić, J., Klisić, A., Kotur-Stevuljević, J., Delogu, G., Gjorgieva Ackova, D., Kostić, K., Dettori, M. A., Fabbri, D., Carta, P.,& Saso, L.. (2023). Natural and natural-like polyphenol compounds: in vitro antioxidant activity and potential for therapeutic application. in Archives of Medical Science
Termedia Publishing House., 19(3), 651-671.
https://doi.org/10.5114/aoms/135379

Brborić J, Klisić A, Kotur-Stevuljević J, Delogu G, Gjorgieva Ackova D, Kostić K, Dettori MA, Fabbri D, Carta P, Saso L. Natural and natural-like polyphenol compounds: in vitro antioxidant activity and potential for therapeutic application. in Archives of Medical Science. 2023;19(3):651-671.
doi:10.5114/aoms/135379 .

Brborić, Jasmina, Klisić, Aleksandra, Kotur-Stevuljević, Jelena, Delogu, Giovanna, Gjorgieva Ackova, Darinka, Kostić, Kristina, Dettori, Maria Antonietta, Fabbri, Davide, Carta, Paola, Saso, Luciano, "Natural and natural-like polyphenol compounds: in vitro antioxidant activity and potential for therapeutic application" in Archives of Medical Science, 19, no. 3 (2023):651-671,
https://doi.org/10.5114/aoms/135379 . .

TY  - JOUR
AU  - Banjac, Gorica
AU  - Ardalić, Daniela
AU  - Cabunac, Petar
AU  - Ivanišević, Jasmina
AU  - Vekić, Jelena
AU  - Miković, Željko
AU  - Stefanović, Aleksandra
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4659
AB  - Background/Aim. One of the complications that can oc-
cur during pregnancy is the development of preeclampsia
(PE). The main characteristics of this condition are high
blood pressure and very often signs of kidney damage or
other organ damage. The condition affects 5–7% of all
pregnant women and is one of the main factors of maternal
and perinatal morbidity and mortality worldwide. The aim
of this study was to investigate the structural and functional
modifications of high-density lipoprotein (HDL) particles
during high-risk pregnancies (HRP) for PE development.
Methods. The longitudinal prospective study included a to-
tal of 91 pregnant women with a HRP for developing PE.
Out of this total number, 71 women did not develop PE
until delivery, and this group was designated as the group
without PE (WPE). The rest of the 20 HRP women devel-
oped PE before delivery and were designated as the PE
group. The blood was sampled toward the end of each tri-
mester and before the delivery. The distribution of HDL
particles was determined by the vertical 3–31% polyacryla-
mide gradient gel electrophoresis method. The antioxidative capacity of HDL particles was measured by the activity of
the HDL-associated enzyme – paraoxonase 1 (PON1).
PON1 activity was determined by the method of kinetic
spectrophotometry from serum samples. Results. The re-
sults have shown that the proportions of HDL 2b particles
significantly increased in the 2nd trimester (p ˂ 0.05) and
remained increased until the end of pregnancy in the WPE
group. PON1 activity was significantly higher in the 3 rd tri-
mester (p < 0.05) of the WPE group. In the PE group, we
found that the proportions of HDL 3a particles significantly
decreased in the 2 nd trimester (p < 0.05) and remained de-
creased until the end of pregnancy. PON1 activity has not
changed in the PE group during pregnancy. Conclusion.
Dyslipidemia in pregnancy could be associated with differ-
ent modifications of HDL particles. The adaptive pregnancy
mechanisms expressed as a functional modification of HDL
particles in pregnant women who develop PE seem inade-
quate and, therefore, lose their atheroprotective role.
AB  - Uvod/Cilj. Jedna od komplikacija koja se može javiti
tokom trudnoće je razvoj preeklampsije (PE). Glavne
karakteristike ovog stanja su visok krvni pritisak i vrlo često
znaci oštećenja bubrega ili drugih organa. Ovo stanje
pogađa 5–7% svih trudnica i jedan je od glavnih faktora
morbiditeta i mortaliteta trudnica i fetusa ili novorođenčadi.
Cilj ovog rada bio je ispitivanje strukturnih i funkcionalnih
modifikacija lipoproteinskih čestica visoke gustine (high-
density lipoprotein – HDL) u trudnoćama sa visokim rizikom
(TVR) za razvoj PE. Metode. U longitudinalnoj
prospektivnoj studiji učestvovala je ukupno 91 trudnica sa
TVR od razvoja PE. Od ukupnog broja trudnica, kod njih 71, PE se nije razvila do kraja trudnoće i ova grupa je
označena kao grupa bez PE (BPE). Kod preostalih 20 žena
sa TVR se razvila PE do porođaja, i one su svrstane u grupu
PE. Krv je uzimana za analizu na kraju svakog trimestra i
pred porođaj. Raspodela HDL čestica je određivana
metodom vertikalne elektroforeze u 3–31% gradijentu
poliakrilamidnog gela. Antioksidativni kapacitet HDL
čestica je određivan na osnovu aktivnosti enzima
paraoksonaze 1 (PON1) vezanog za HDL. Aktivnost
PON1 u serumu je određivana metodom kinetičke
spektrofotometrije. Rezultati. Rezultati su pokazali da se
udeo HDL 2b čestica značajno povećao u drugom trimestru
(p < 0,05) i ostao je povećan do kraja trudnoće u grupi BPE.
Aktivnost PON1 bila je značajno veća u trećem trimestru (p < 0,05) kod ove grupe trudnica. Udeo HDL 3a
čestica se značajno smanjio u drugom trimestru u PE grupi
trudnica (p < 0,05) i ostao je snižen do kraja trudno će.
Aktivnost PON1 enzima u PE gripi se nije menjala u toku
trudnoće. Zaključak. Dislipidemija u trudnoći može biti
posledica različitih modifikacija HDL čestica. Strukturne i
funkcionalne modifikacije HDL čestica, kao jedan od adaptivnih mehanizama, kod trudnica kod kojih se razvila
PE, nisu adekvatne i kao takve gube svoju ateroprotektivnu
ulogu.
PB  - Univerzitet odbrane - Vojnomedicinska akademija, Medicinski fakultet
T2  - Vojnosanitetski pregled
T1  - Altered high-density lipoprotein particle structure and antioxidant capacity in preeclampsia
T1  - Izmenjena struktura i antioksidativni kapacitet lipoproteinskih čestica visoke gustine u preeklampsiji
VL  - 80
IS  - 2
SP  - 121
EP  - 127
DO  - 10.2298/VSP210413010B
ER  - 

@article{
author = "Banjac, Gorica and Ardalić, Daniela and Cabunac, Petar and Ivanišević, Jasmina and Vekić, Jelena and Miković, Željko and Stefanović, Aleksandra",
year = "2023",
abstract = "Background/Aim. One of the complications that can oc-
cur during pregnancy is the development of preeclampsia
(PE). The main characteristics of this condition are high
blood pressure and very often signs of kidney damage or
other organ damage. The condition affects 5–7% of all
pregnant women and is one of the main factors of maternal
and perinatal morbidity and mortality worldwide. The aim
of this study was to investigate the structural and functional
modifications of high-density lipoprotein (HDL) particles
during high-risk pregnancies (HRP) for PE development.
Methods. The longitudinal prospective study included a to-
tal of 91 pregnant women with a HRP for developing PE.
Out of this total number, 71 women did not develop PE
until delivery, and this group was designated as the group
without PE (WPE). The rest of the 20 HRP women devel-
oped PE before delivery and were designated as the PE
group. The blood was sampled toward the end of each tri-
mester and before the delivery. The distribution of HDL
particles was determined by the vertical 3–31% polyacryla-
mide gradient gel electrophoresis method. The antioxidative capacity of HDL particles was measured by the activity of
the HDL-associated enzyme – paraoxonase 1 (PON1).
PON1 activity was determined by the method of kinetic
spectrophotometry from serum samples. Results. The re-
sults have shown that the proportions of HDL 2b particles
significantly increased in the 2nd trimester (p ˂ 0.05) and
remained increased until the end of pregnancy in the WPE
group. PON1 activity was significantly higher in the 3 rd tri-
mester (p < 0.05) of the WPE group. In the PE group, we
found that the proportions of HDL 3a particles significantly
decreased in the 2 nd trimester (p < 0.05) and remained de-
creased until the end of pregnancy. PON1 activity has not
changed in the PE group during pregnancy. Conclusion.
Dyslipidemia in pregnancy could be associated with differ-
ent modifications of HDL particles. The adaptive pregnancy
mechanisms expressed as a functional modification of HDL
particles in pregnant women who develop PE seem inade-
quate and, therefore, lose their atheroprotective role., Uvod/Cilj. Jedna od komplikacija koja se može javiti
tokom trudnoće je razvoj preeklampsije (PE). Glavne
karakteristike ovog stanja su visok krvni pritisak i vrlo često
znaci oštećenja bubrega ili drugih organa. Ovo stanje
pogađa 5–7% svih trudnica i jedan je od glavnih faktora
morbiditeta i mortaliteta trudnica i fetusa ili novorođenčadi.
Cilj ovog rada bio je ispitivanje strukturnih i funkcionalnih
modifikacija lipoproteinskih čestica visoke gustine (high-
density lipoprotein – HDL) u trudnoćama sa visokim rizikom
(TVR) za razvoj PE. Metode. U longitudinalnoj
prospektivnoj studiji učestvovala je ukupno 91 trudnica sa
TVR od razvoja PE. Od ukupnog broja trudnica, kod njih 71, PE se nije razvila do kraja trudnoće i ova grupa je
označena kao grupa bez PE (BPE). Kod preostalih 20 žena
sa TVR se razvila PE do porođaja, i one su svrstane u grupu
PE. Krv je uzimana za analizu na kraju svakog trimestra i
pred porođaj. Raspodela HDL čestica je određivana
metodom vertikalne elektroforeze u 3–31% gradijentu
poliakrilamidnog gela. Antioksidativni kapacitet HDL
čestica je određivan na osnovu aktivnosti enzima
paraoksonaze 1 (PON1) vezanog za HDL. Aktivnost
PON1 u serumu je određivana metodom kinetičke
spektrofotometrije. Rezultati. Rezultati su pokazali da se
udeo HDL 2b čestica značajno povećao u drugom trimestru
(p < 0,05) i ostao je povećan do kraja trudnoće u grupi BPE.
Aktivnost PON1 bila je značajno veća u trećem trimestru (p < 0,05) kod ove grupe trudnica. Udeo HDL 3a
čestica se značajno smanjio u drugom trimestru u PE grupi
trudnica (p < 0,05) i ostao je snižen do kraja trudno će.
Aktivnost PON1 enzima u PE gripi se nije menjala u toku
trudnoće. Zaključak. Dislipidemija u trudnoći može biti
posledica različitih modifikacija HDL čestica. Strukturne i
funkcionalne modifikacije HDL čestica, kao jedan od adaptivnih mehanizama, kod trudnica kod kojih se razvila
PE, nisu adekvatne i kao takve gube svoju ateroprotektivnu
ulogu.",
publisher = "Univerzitet odbrane - Vojnomedicinska akademija, Medicinski fakultet",
journal = "Vojnosanitetski pregled",
title = "Altered high-density lipoprotein particle structure and antioxidant capacity in preeclampsia, Izmenjena struktura i antioksidativni kapacitet lipoproteinskih čestica visoke gustine u preeklampsiji",
volume = "80",
number = "2",
pages = "121-127",
doi = "10.2298/VSP210413010B"
}

Banjac, G., Ardalić, D., Cabunac, P., Ivanišević, J., Vekić, J., Miković, Ž.,& Stefanović, A.. (2023). Altered high-density lipoprotein particle structure and antioxidant capacity in preeclampsia. in Vojnosanitetski pregled
Univerzitet odbrane - Vojnomedicinska akademija, Medicinski fakultet., 80(2), 121-127.
https://doi.org/10.2298/VSP210413010B

Banjac G, Ardalić D, Cabunac P, Ivanišević J, Vekić J, Miković Ž, Stefanović A. Altered high-density lipoprotein particle structure and antioxidant capacity in preeclampsia. in Vojnosanitetski pregled. 2023;80(2):121-127.
doi:10.2298/VSP210413010B .

Banjac, Gorica, Ardalić, Daniela, Cabunac, Petar, Ivanišević, Jasmina, Vekić, Jelena, Miković, Željko, Stefanović, Aleksandra, "Altered high-density lipoprotein particle structure and antioxidant capacity in preeclampsia" in Vojnosanitetski pregled, 80, no. 2 (2023):121-127,
https://doi.org/10.2298/VSP210413010B . .

TY  - JOUR
AU  - Sopić, Miron
AU  - Ninić, Ana
AU  - Ostanek, Barbara
AU  - Bojanin, Dragana
AU  - Milenković, Tatjana
AU  - Munjas, Jelena
AU  - Mihajlović, Marija
AU  - Vekić, Jelena
AU  - Marc, Janja
AU  - Spasojević-Kalimanovska, Vesna
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4255
AB  - Background: Type 1 diabetes mellitus (T1DM) is one of the
most common endocrine diseases in children. T-cell autore-
activity toward b-cells is controlled by significant changes in
metabolism of T cells. Mammalian target of rapamycin
(mTOR) is an important intracellular regulator of metabolism
and cell growth. MAPK/MAK/MRK overlapping kinase 1
(MOK1) is one of the less known regulators of mTOR. We
sought to investigate if MOK1 and mTOR mRNA levels in
peripheral blood mononuclear cells (PBMCs) of T1DM pedi-
atric patients are different compared to healthy subjects.
Methods: This study included 172 adolescents with T1DM
and 36 healthy adolescent volunteers designated for control
group (CG). MOK1 and mTOR mRNA levels were deter-
mined in PBMCs by qPCR.
Results: T1DM patients have significant downregulation of
MOK1 mRNA levels in PBMCs compared CG (P=0.018),
while there was no significant difference in mTOR mRNA
levels (P=0.891). Furthermore, in T1DM patients, MOK1
significantly correlated with age, triglycerides and mTOR,
while mTOR correlated significantly with BMI and systolic
blood pressure. Overweight T1DM subjects had significantly
lower MOK1 (P=0.034) and mTOR (P=0.017) mRNA
levels, together with significantly higher levels of systolic
blood pressure (P<0.001), total cholesterol (P=0.001),
LDL-cholesterol (P=0.001) and CRP (P<0.001). Multi-
variate analysis showed that MOK1 was independently
negatively associated with T1DM when adjusted for sex, age, HDL-C and CRP (OR=0.417 (95%CI: 0.175–0.997),
p=0.049).
Conclusions: Our study demonstrated for the first time that
T1DM is associated with MOK1 downregulation. In addition,
downregulation of both mTOR and MOK1 gene expressions
was associated with cardiovascular risk factors in overweight
T1DM patients.
AB  - Uvod: Dijabetes melitus tip 1 (T1DM) jedno je od najčešćih endokrinih oboljenja kod dece. Autoreaktivnost T-ćelija prema b-ćelijama kontroliše se značajnim promenama u metabolizmu T ćelija. Cilj delovanja rapamicina kod sisara je važan unutarćelijski regulator metabolizma i rasta ćelija. MAPK/MAK/MRK preklapajuće kinaze koja se preklapa (MOK1) jedan je od manje poznatih regulatora mTOR-a. Cilj istraživanja je bio da se ispita da li su nivoi iRNK MOK1 i mTOR u mononuklearnim ćelijama periferne krvi različiti kod pedijatrijskih pacijenata sa T1DM u odnosu na zdrave ispitanike. Metode: Ovo istraživanje je obuhvatilo 172 adolescenta sa T1DM i 36 zdravih adolescenata dobrovoljaca koji su činili kontrolnu grupu (CG). Nivoi MOK1 i mTOR mRNA određeni su u PBMC-ima pomoću qPCR-a. Rezultati: Pacijenti sa T1DM imali su značajno niže nivoe iRNK MOK1 u PBMC u odnosu na CG, dok razlike u nivoima iRNK mTOR nisu bile značajne (P = 0,891). Štaviše, kod pacijenata sa T1DM, MOK1 je značajno korelirao sa godinama, trigliceri dima i mTOR, dok je mTOR značajno korelirao sa BMI i sistolnim krvnim pritiskom. Ispitanici sa prekomernom težinom T1DM imali su značajno niže nivoe iRNK MOK1 (P = 0,034) i mTOR (P = 0,017), zajedno sa značajno većim nivoima sistolnog krvnog pritiska (P <0,001), ukupnog holesterola (P = 0,001), LDL-holesterola (P = 0,001) i CRP (P <0,001). Multivarijantna analiza je pokazala da je MOK1 nezavisno negativno povezan sa T1DM kada je prilagođen polu, starosti, HDL-C i CRP (OR = 0,417 (95%CI: 0,175-0,997), p = 0,049). Zaključak: Naša studija je prva koja je pokazala da je T1DM udružen sa nishodnom regulacijom MOK1. Pored toga, snižena regulacija ekspresije gena mTOR i MOK1 bila je povezana sa kardiovaskularnim faktorima rizika kod pacije nata sa T1DM sa prekomernom težinom.
PB  - Beograd : Društvo medicinskih biohemičara Srbije
T2  - Journal of Medical Biochemistry
T1  - Downregulation of MAPK/MAK/MRK overlapping kinase 1 in peripheral blood mononuclear cells of pediatric patients with type 1 diabetes mellitus
T1  - Nishodna regulacija MAPK/MAK/MRK preklapajuće kinaze u mononuklearnim ćelijama periferne krvi pedijatrijskih pacijenata sa tip1 diiabetes mellitus-om
VL  - 41
IS  - 3
SP  - 282
EP  - 289
DO  - 10.5937/jomb0-33220
ER  - 

@article{
author = "Sopić, Miron and Ninić, Ana and Ostanek, Barbara and Bojanin, Dragana and Milenković, Tatjana and Munjas, Jelena and Mihajlović, Marija and Vekić, Jelena and Marc, Janja and Spasojević-Kalimanovska, Vesna",
year = "2022",
abstract = "Background: Type 1 diabetes mellitus (T1DM) is one of the
most common endocrine diseases in children. T-cell autore-
activity toward b-cells is controlled by significant changes in
metabolism of T cells. Mammalian target of rapamycin
(mTOR) is an important intracellular regulator of metabolism
and cell growth. MAPK/MAK/MRK overlapping kinase 1
(MOK1) is one of the less known regulators of mTOR. We
sought to investigate if MOK1 and mTOR mRNA levels in
peripheral blood mononuclear cells (PBMCs) of T1DM pedi-
atric patients are different compared to healthy subjects.
Methods: This study included 172 adolescents with T1DM
and 36 healthy adolescent volunteers designated for control
group (CG). MOK1 and mTOR mRNA levels were deter-
mined in PBMCs by qPCR.
Results: T1DM patients have significant downregulation of
MOK1 mRNA levels in PBMCs compared CG (P=0.018),
while there was no significant difference in mTOR mRNA
levels (P=0.891). Furthermore, in T1DM patients, MOK1
significantly correlated with age, triglycerides and mTOR,
while mTOR correlated significantly with BMI and systolic
blood pressure. Overweight T1DM subjects had significantly
lower MOK1 (P=0.034) and mTOR (P=0.017) mRNA
levels, together with significantly higher levels of systolic
blood pressure (P<0.001), total cholesterol (P=0.001),
LDL-cholesterol (P=0.001) and CRP (P<0.001). Multi-
variate analysis showed that MOK1 was independently
negatively associated with T1DM when adjusted for sex, age, HDL-C and CRP (OR=0.417 (95%CI: 0.175–0.997),
p=0.049).
Conclusions: Our study demonstrated for the first time that
T1DM is associated with MOK1 downregulation. In addition,
downregulation of both mTOR and MOK1 gene expressions
was associated with cardiovascular risk factors in overweight
T1DM patients., Uvod: Dijabetes melitus tip 1 (T1DM) jedno je od najčešćih endokrinih oboljenja kod dece. Autoreaktivnost T-ćelija prema b-ćelijama kontroliše se značajnim promenama u metabolizmu T ćelija. Cilj delovanja rapamicina kod sisara je važan unutarćelijski regulator metabolizma i rasta ćelija. MAPK/MAK/MRK preklapajuće kinaze koja se preklapa (MOK1) jedan je od manje poznatih regulatora mTOR-a. Cilj istraživanja je bio da se ispita da li su nivoi iRNK MOK1 i mTOR u mononuklearnim ćelijama periferne krvi različiti kod pedijatrijskih pacijenata sa T1DM u odnosu na zdrave ispitanike. Metode: Ovo istraživanje je obuhvatilo 172 adolescenta sa T1DM i 36 zdravih adolescenata dobrovoljaca koji su činili kontrolnu grupu (CG). Nivoi MOK1 i mTOR mRNA određeni su u PBMC-ima pomoću qPCR-a. Rezultati: Pacijenti sa T1DM imali su značajno niže nivoe iRNK MOK1 u PBMC u odnosu na CG, dok razlike u nivoima iRNK mTOR nisu bile značajne (P = 0,891). Štaviše, kod pacijenata sa T1DM, MOK1 je značajno korelirao sa godinama, trigliceri dima i mTOR, dok je mTOR značajno korelirao sa BMI i sistolnim krvnim pritiskom. Ispitanici sa prekomernom težinom T1DM imali su značajno niže nivoe iRNK MOK1 (P = 0,034) i mTOR (P = 0,017), zajedno sa značajno većim nivoima sistolnog krvnog pritiska (P <0,001), ukupnog holesterola (P = 0,001), LDL-holesterola (P = 0,001) i CRP (P <0,001). Multivarijantna analiza je pokazala da je MOK1 nezavisno negativno povezan sa T1DM kada je prilagođen polu, starosti, HDL-C i CRP (OR = 0,417 (95%CI: 0,175-0,997), p = 0,049). Zaključak: Naša studija je prva koja je pokazala da je T1DM udružen sa nishodnom regulacijom MOK1. Pored toga, snižena regulacija ekspresije gena mTOR i MOK1 bila je povezana sa kardiovaskularnim faktorima rizika kod pacije nata sa T1DM sa prekomernom težinom.",
publisher = "Beograd : Društvo medicinskih biohemičara Srbije",
journal = "Journal of Medical Biochemistry",
title = "Downregulation of MAPK/MAK/MRK overlapping kinase 1 in peripheral blood mononuclear cells of pediatric patients with type 1 diabetes mellitus, Nishodna regulacija MAPK/MAK/MRK preklapajuće kinaze u mononuklearnim ćelijama periferne krvi pedijatrijskih pacijenata sa tip1 diiabetes mellitus-om",
volume = "41",
number = "3",
pages = "282-289",
doi = "10.5937/jomb0-33220"
}

Sopić, M., Ninić, A., Ostanek, B., Bojanin, D., Milenković, T., Munjas, J., Mihajlović, M., Vekić, J., Marc, J.,& Spasojević-Kalimanovska, V.. (2022). Downregulation of MAPK/MAK/MRK overlapping kinase 1 in peripheral blood mononuclear cells of pediatric patients with type 1 diabetes mellitus. in Journal of Medical Biochemistry
Beograd : Društvo medicinskih biohemičara Srbije., 41(3), 282-289.
https://doi.org/10.5937/jomb0-33220

Sopić M, Ninić A, Ostanek B, Bojanin D, Milenković T, Munjas J, Mihajlović M, Vekić J, Marc J, Spasojević-Kalimanovska V. Downregulation of MAPK/MAK/MRK overlapping kinase 1 in peripheral blood mononuclear cells of pediatric patients with type 1 diabetes mellitus. in Journal of Medical Biochemistry. 2022;41(3):282-289.
doi:10.5937/jomb0-33220 .

Sopić, Miron, Ninić, Ana, Ostanek, Barbara, Bojanin, Dragana, Milenković, Tatjana, Munjas, Jelena, Mihajlović, Marija, Vekić, Jelena, Marc, Janja, Spasojević-Kalimanovska, Vesna, "Downregulation of MAPK/MAK/MRK overlapping kinase 1 in peripheral blood mononuclear cells of pediatric patients with type 1 diabetes mellitus" in Journal of Medical Biochemistry, 41, no. 3 (2022):282-289,
https://doi.org/10.5937/jomb0-33220 . .

TY  - JOUR
AU  - Terzić, Dragana
AU  - Tadić, Ivana
AU  - Lakić, Dragana
AU  - Odalović, Marina
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4283
AB  - Healthcare  professionals  should  serve  as  promoters  of  ra-tional  antibiotic  use  in  attempt  to  decrease  antibiotics  misuse  within  the  process  of  self-medication. Current  pilot  study  was  undertaken  with  the  aim  to  identify  potential  predictors  of  self-medication with antibiotics (SMA) and to describe SMA practice among nursing students in Serbia. Data have been collected dur-ing  the  period  March-May,  2016  in  Medical  school  of  bachelor  degree, in Belgrade. Self-reported questionnaire served as a data source.  Descriptive  statistic  was  used  to  analyse  study  sample  characteristics.  Chi-square  test  was  used  to  test  differences  be-tween groups. Study sample have included 138 participants. Al-most half of them, 43.8%, practice SMA. Life style (smoking, al-cohol consumption, sleeping habits and physical activity) and so-cio-demographic  characteristics  (excepted  school  grade)  were  not  shown  as  SMA  determinants.  Time  &  money  savings  were  stated as the most frequent reason for SMA, while common cold, sore  throat  and  cough  were  the  most  common  conditions  cured  through SMA. Pharmacists’ recommendations and previous pos-itive experience were specified as the most important in the pro-cess of antibiotics selection, indicated by 50.0% and 37.5% par-ticipants,  respectively.  Amoxicillin  was  the  most  frequently  used  antibiotic in SMA, used by 50% of participants who practice SMA. High  proportion  of  SMA  and  observed  practice  among  nursing  students in Serbia call for efforts with regards to relevant educa-tion  about  rational  antibiotic  use,  actual  clinical  guidelines  and  potential consequences of misuse.
AB  - Zdravstveni  stručnjaci  bi  trebalo  da  služe  kao  pokretači  ra-cionalne  upotrebe  antibiotika  u  pokušaju  da  se  smanji  zloupo-treba  antibiotika  kroz  proces  samomedikacije.  Pilot  studija  je  sprovedena   sa   ciljem   da   se   utvrde   potencijalni   prediktori   samomedikacije  antibioticima  (SMA)  i  da  se  opiše  praksa  SMA  među studentima sestrinstva u Srbiji. Podaci su sakupljeni tokom perioda  Mart-Maj  2016,  u  Višoj  školi  strukovnih  studija  u  Be-ogradu,  Srbija.  Za  sakupljanje  podataka  korišćen  je  upitnih  za samo-popunjavanje.  U  analizi  karakteristika  ispitivanog  uzorka  korišćena je deskriptivna statistika. Hi-kvadrat test je korišćen za ispitivanje razlike između grupa. Ukupan broj učesnika u studiji bio je 138. Gotovo polovina, 43,8% je koristila SMA. Životni stil (pušenje,  konzumiranje  alkohola,  navike  u  pogledu  sna,  fizička aktivnost)   i   socio-demografske   karakteristike   (izuzev   godine   studija)  nisu  pokazane  kao  SMA.  Uštede  u  vremenu  i  novcu  su  navedene  kao  najčešći  razlog  za  SMA,  dok  su  prehlada,  upala grla  i  kašalj  najčešće  indikacije  tretirane  kroz  SMA.  Preporuke  farmaceuta  i  prethodno  pozitivno  iskustvo  su  navedeni  kao  najznačajniji faktori u odabiru antibiotika, što je tvrdilo 50,0% i 37,5% učesnika, redom. Amoksicilin je bio najčešće korišćen an-tibiotik koji je koristilo 50% učesnika kroz SMA. Visok procenat SMA  i  uočena  praksa  među  studentima  sestrinstva  u  Srbiji ukazuje na potrebe za relevantnom edukacijom u vezi sa racion-alnom  upotrebom  antibiotika,  aktuelnim  preporukama  u  klin-ičkim  vodičima  i  potencijalnim  posledicama  nepoštovanja  datih  preporuka.
PB  - University of Kragujevac, Faculty of Science
T2  - Serbian Journal of Experimental and Clinical Research
T1  - Self-medication with antibiotics among nursing students in Serbia: pilot study
T1  - Samomedikacija antibioticima među studentima sesrtinstva u Srbiji: pilot studija
VL  - 23
IS  - 3
SP  - 229
EP  - 235
DO  - 10.2478/sjecr-2019-0055
ER  - 

@article{
author = "Terzić, Dragana and Tadić, Ivana and Lakić, Dragana and Odalović, Marina",
year = "2022",
abstract = "Healthcare  professionals  should  serve  as  promoters  of  ra-tional  antibiotic  use  in  attempt  to  decrease  antibiotics  misuse  within  the  process  of  self-medication. Current  pilot  study  was  undertaken  with  the  aim  to  identify  potential  predictors  of  self-medication with antibiotics (SMA) and to describe SMA practice among nursing students in Serbia. Data have been collected dur-ing  the  period  March-May,  2016  in  Medical  school  of  bachelor  degree, in Belgrade. Self-reported questionnaire served as a data source.  Descriptive  statistic  was  used  to  analyse  study  sample  characteristics.  Chi-square  test  was  used  to  test  differences  be-tween groups. Study sample have included 138 participants. Al-most half of them, 43.8%, practice SMA. Life style (smoking, al-cohol consumption, sleeping habits and physical activity) and so-cio-demographic  characteristics  (excepted  school  grade)  were  not  shown  as  SMA  determinants.  Time  &  money  savings  were  stated as the most frequent reason for SMA, while common cold, sore  throat  and  cough  were  the  most  common  conditions  cured  through SMA. Pharmacists’ recommendations and previous pos-itive experience were specified as the most important in the pro-cess of antibiotics selection, indicated by 50.0% and 37.5% par-ticipants,  respectively.  Amoxicillin  was  the  most  frequently  used  antibiotic in SMA, used by 50% of participants who practice SMA. High  proportion  of  SMA  and  observed  practice  among  nursing  students in Serbia call for efforts with regards to relevant educa-tion  about  rational  antibiotic  use,  actual  clinical  guidelines  and  potential consequences of misuse., Zdravstveni  stručnjaci  bi  trebalo  da  služe  kao  pokretači  ra-cionalne  upotrebe  antibiotika  u  pokušaju  da  se  smanji  zloupo-treba  antibiotika  kroz  proces  samomedikacije.  Pilot  studija  je  sprovedena   sa   ciljem   da   se   utvrde   potencijalni   prediktori   samomedikacije  antibioticima  (SMA)  i  da  se  opiše  praksa  SMA  među studentima sestrinstva u Srbiji. Podaci su sakupljeni tokom perioda  Mart-Maj  2016,  u  Višoj  školi  strukovnih  studija  u  Be-ogradu,  Srbija.  Za  sakupljanje  podataka  korišćen  je  upitnih  za samo-popunjavanje.  U  analizi  karakteristika  ispitivanog  uzorka  korišćena je deskriptivna statistika. Hi-kvadrat test je korišćen za ispitivanje razlike između grupa. Ukupan broj učesnika u studiji bio je 138. Gotovo polovina, 43,8% je koristila SMA. Životni stil (pušenje,  konzumiranje  alkohola,  navike  u  pogledu  sna,  fizička aktivnost)   i   socio-demografske   karakteristike   (izuzev   godine   studija)  nisu  pokazane  kao  SMA.  Uštede  u  vremenu  i  novcu  su  navedene  kao  najčešći  razlog  za  SMA,  dok  su  prehlada,  upala grla  i  kašalj  najčešće  indikacije  tretirane  kroz  SMA.  Preporuke  farmaceuta  i  prethodno  pozitivno  iskustvo  su  navedeni  kao  najznačajniji faktori u odabiru antibiotika, što je tvrdilo 50,0% i 37,5% učesnika, redom. Amoksicilin je bio najčešće korišćen an-tibiotik koji je koristilo 50% učesnika kroz SMA. Visok procenat SMA  i  uočena  praksa  među  studentima  sestrinstva  u  Srbiji ukazuje na potrebe za relevantnom edukacijom u vezi sa racion-alnom  upotrebom  antibiotika,  aktuelnim  preporukama  u  klin-ičkim  vodičima  i  potencijalnim  posledicama  nepoštovanja  datih  preporuka.",
publisher = "University of Kragujevac, Faculty of Science",
journal = "Serbian Journal of Experimental and Clinical Research",
title = "Self-medication with antibiotics among nursing students in Serbia: pilot study, Samomedikacija antibioticima među studentima sesrtinstva u Srbiji: pilot studija",
volume = "23",
number = "3",
pages = "229-235",
doi = "10.2478/sjecr-2019-0055"
}

Terzić, D., Tadić, I., Lakić, D.,& Odalović, M.. (2022). Self-medication with antibiotics among nursing students in Serbia: pilot study. in Serbian Journal of Experimental and Clinical Research
University of Kragujevac, Faculty of Science., 23(3), 229-235.
https://doi.org/10.2478/sjecr-2019-0055

Terzić D, Tadić I, Lakić D, Odalović M. Self-medication with antibiotics among nursing students in Serbia: pilot study. in Serbian Journal of Experimental and Clinical Research. 2022;23(3):229-235.
doi:10.2478/sjecr-2019-0055 .

Terzić, Dragana, Tadić, Ivana, Lakić, Dragana, Odalović, Marina, "Self-medication with antibiotics among nursing students in Serbia: pilot study" in Serbian Journal of Experimental and Clinical Research, 23, no. 3 (2022):229-235,
https://doi.org/10.2478/sjecr-2019-0055 . .

TY  - JOUR
AU  - Roksandić Milenković, M.
AU  - Klisić, Aleksandra
AU  - Ceriman, V.
AU  - Kotur-Stevuljević, Jelena
AU  - Savić-Vujović, Katarina
AU  - Mirkov, D.
AU  - Gajić, M.
AU  - Ilić, B.
AU  - Dimić, N.
AU  - Samardžić, N.
AU  - Jovanović, D.
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4171
AB  - Objective: The pathophysiological mechanisms of idiopathic pulmonary fibrosis (IPF) are not well elucidated. It is assumed that oxidative stress and inflammation are the key underlying culprits for its onset and progression. To gain deeper insight into these processes, we have evaluated several oxidative stress parameters, inflammation markers [i.e., high sensitivity C-reactive protein (hsCRP), serum amyloid A1 (SAA1)], soluble programmed cell death-ligand 1 (sPD-L1), and 25-hydroxyvitamin D [25(OH)D] in IPF patients. Patients and Methods: Biochemistry analyses were done in 30 consecutive IPF patients and 30 age and gender-matched healthy control group (CG). Results: IPF patients had significantly higher advanced oxidation protein products (p<0.001), pro-oxidant-antioxidant balance (p=0.010), total oxidative status (p<0.001), and ischemia modified albumin (p<0.001) compared to CG. Lower total antioxidant status and total sulfhydryl groups (tSGH) and significantly higher sPD-L1, hsCRP (p<0.001 for all), SAA1 proteins (p=0.014) and [25(OH)D] severe deficiency [11.0 (9.6-15.1) nmol/L] in IPF patients compared to CG were observed. Paraoxonase 1 activity and hsCRP level were lower, while tSHG and sPD-L1 were higher in IPF patients with more severe disease (i.e., II+III stage compared to I stage, p<0.05 for all). Conclusions: IPF patients are in a state of profound oxidative stress compared to healthy people. The inflammatory component of the disease was confirmed by higher hsCRP and SAA1, but lower [25(OH)D] in IPF than in healthy people. Also, higher levels of sPD-L1 in patients with IPF compared to healthy individuals suggest that sPD-L1 may have a significant role in immune response in IPF. © 2022 Verduci Editore s.r.l. All rights reserved.
PB  - Verduci Editore s.r.l
T2  - European Review for Medical and Pharmacological Sciences
T1  - Oxidative stress and inflammation parameters-novel biomarkers for idiopathic pulmonary fibrosis
VL  - 26
IS  - 3
SP  - 927
EP  - 934
DO  - 10.26355/eurrev_202202_28002
ER  - 

@article{
author = "Roksandić Milenković, M. and Klisić, Aleksandra and Ceriman, V. and Kotur-Stevuljević, Jelena and Savić-Vujović, Katarina and Mirkov, D. and Gajić, M. and Ilić, B. and Dimić, N. and Samardžić, N. and Jovanović, D.",
year = "2022",
abstract = "Objective: The pathophysiological mechanisms of idiopathic pulmonary fibrosis (IPF) are not well elucidated. It is assumed that oxidative stress and inflammation are the key underlying culprits for its onset and progression. To gain deeper insight into these processes, we have evaluated several oxidative stress parameters, inflammation markers [i.e., high sensitivity C-reactive protein (hsCRP), serum amyloid A1 (SAA1)], soluble programmed cell death-ligand 1 (sPD-L1), and 25-hydroxyvitamin D [25(OH)D] in IPF patients. Patients and Methods: Biochemistry analyses were done in 30 consecutive IPF patients and 30 age and gender-matched healthy control group (CG). Results: IPF patients had significantly higher advanced oxidation protein products (p<0.001), pro-oxidant-antioxidant balance (p=0.010), total oxidative status (p<0.001), and ischemia modified albumin (p<0.001) compared to CG. Lower total antioxidant status and total sulfhydryl groups (tSGH) and significantly higher sPD-L1, hsCRP (p<0.001 for all), SAA1 proteins (p=0.014) and [25(OH)D] severe deficiency [11.0 (9.6-15.1) nmol/L] in IPF patients compared to CG were observed. Paraoxonase 1 activity and hsCRP level were lower, while tSHG and sPD-L1 were higher in IPF patients with more severe disease (i.e., II+III stage compared to I stage, p<0.05 for all). Conclusions: IPF patients are in a state of profound oxidative stress compared to healthy people. The inflammatory component of the disease was confirmed by higher hsCRP and SAA1, but lower [25(OH)D] in IPF than in healthy people. Also, higher levels of sPD-L1 in patients with IPF compared to healthy individuals suggest that sPD-L1 may have a significant role in immune response in IPF. © 2022 Verduci Editore s.r.l. All rights reserved.",
publisher = "Verduci Editore s.r.l",
journal = "European Review for Medical and Pharmacological Sciences",
title = "Oxidative stress and inflammation parameters-novel biomarkers for idiopathic pulmonary fibrosis",
volume = "26",
number = "3",
pages = "927-934",
doi = "10.26355/eurrev_202202_28002"
}

Roksandić Milenković, M., Klisić, A., Ceriman, V., Kotur-Stevuljević, J., Savić-Vujović, K., Mirkov, D., Gajić, M., Ilić, B., Dimić, N., Samardžić, N.,& Jovanović, D.. (2022). Oxidative stress and inflammation parameters-novel biomarkers for idiopathic pulmonary fibrosis. in European Review for Medical and Pharmacological Sciences
Verduci Editore s.r.l., 26(3), 927-934.
https://doi.org/10.26355/eurrev_202202_28002

Roksandić Milenković M, Klisić A, Ceriman V, Kotur-Stevuljević J, Savić-Vujović K, Mirkov D, Gajić M, Ilić B, Dimić N, Samardžić N, Jovanović D. Oxidative stress and inflammation parameters-novel biomarkers for idiopathic pulmonary fibrosis. in European Review for Medical and Pharmacological Sciences. 2022;26(3):927-934.
doi:10.26355/eurrev_202202_28002 .

Roksandić Milenković, M., Klisić, Aleksandra, Ceriman, V., Kotur-Stevuljević, Jelena, Savić-Vujović, Katarina, Mirkov, D., Gajić, M., Ilić, B., Dimić, N., Samardžić, N., Jovanović, D., "Oxidative stress and inflammation parameters-novel biomarkers for idiopathic pulmonary fibrosis" in European Review for Medical and Pharmacological Sciences, 26, no. 3 (2022):927-934,
https://doi.org/10.26355/eurrev_202202_28002 . .

TY  - JOUR
AU  - Antonić, Tamara
AU  - Ardalić, Daniela
AU  - Vladimirov, Sandra
AU  - Banjac, Gorica
AU  - Cabunac, Petar
AU  - Zeljković, Aleksandra
AU  - Karadžov-Orlić, Natasa
AU  - Spasojević-Kalimanovska, Vesna
AU  - Miković, Željko
AU  - Stefanović, Aleksandra
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4023
AB  - Introduction The link between preeclampsia and dyslipidemia has been established. Even though lipid profile parameters have been intensively investigated in the pathology of preeclampsia, their accurate molecular mechanisms of action have not been fully decoded. Objectives We aimed to identify the specifics of cholesterol metabolism in women affected by late‑onset preeclampsia and single out potential biomarkers associated with late‑onset syndrome. Patient s and methods A total of 90 pregnant women with a priori risk for preeclampsia were monitored at 4 time points during gestation and, based on the outcome of pregnancy, they were classified into the high‑risk group (70 women) and the preeclampsia group (20 women). Cholesterol metabolic profiling was done using liquid chromatography‑tandem mass spectrometry. Result s The only significant change in the preeclampsia group was an increase in the lathosterol level (P = 0.001). The first‑trimester lathosterol level was higher in the preeclampsia group compared with the high‑risk group (P = 0.02). Further, in the preeclampsia group, positive correlations were found between desmosterol and β‑sitosterol (ρ = 0.474; P = 0.03) in the third trimester, desmosterol and campesterol changes between the second and the first (ρ = 0.546; P = 0.02), and the third and first trimesters (ρ = 0.754; P <0.001), as well as between the desmosterol and β‑sitosterol differences between the third and first trimesters (ρ = 0.568; P = 0.01). No similar correlations were found in the high‑risk group. Conclusions Late‑onset preeclampsia could be associated with an altered lipid profile. By studying the quantitative metabolic signatures of cholesterol, we might assume that both cholesterol synthesis and absorption are increased, that is, there is an imbalance in the cholesterol homeostasis regulation in women affected by the disease.
PB  - Medycyna Praktyczna Cholerzyn
T2  - Polish Archives of Internal Medicine
T1  - Cholesterol homeostasis is dysregulated in women with preeclampsia
VL  - 131
IS  - 12
DO  - 10.20452/pamw.16144
ER  - 

@article{
author = "Antonić, Tamara and Ardalić, Daniela and Vladimirov, Sandra and Banjac, Gorica and Cabunac, Petar and Zeljković, Aleksandra and Karadžov-Orlić, Natasa and Spasojević-Kalimanovska, Vesna and Miković, Željko and Stefanović, Aleksandra",
year = "2021",
abstract = "Introduction The link between preeclampsia and dyslipidemia has been established. Even though lipid profile parameters have been intensively investigated in the pathology of preeclampsia, their accurate molecular mechanisms of action have not been fully decoded. Objectives We aimed to identify the specifics of cholesterol metabolism in women affected by late‑onset preeclampsia and single out potential biomarkers associated with late‑onset syndrome. Patient s and methods A total of 90 pregnant women with a priori risk for preeclampsia were monitored at 4 time points during gestation and, based on the outcome of pregnancy, they were classified into the high‑risk group (70 women) and the preeclampsia group (20 women). Cholesterol metabolic profiling was done using liquid chromatography‑tandem mass spectrometry. Result s The only significant change in the preeclampsia group was an increase in the lathosterol level (P = 0.001). The first‑trimester lathosterol level was higher in the preeclampsia group compared with the high‑risk group (P = 0.02). Further, in the preeclampsia group, positive correlations were found between desmosterol and β‑sitosterol (ρ = 0.474; P = 0.03) in the third trimester, desmosterol and campesterol changes between the second and the first (ρ = 0.546; P = 0.02), and the third and first trimesters (ρ = 0.754; P <0.001), as well as between the desmosterol and β‑sitosterol differences between the third and first trimesters (ρ = 0.568; P = 0.01). No similar correlations were found in the high‑risk group. Conclusions Late‑onset preeclampsia could be associated with an altered lipid profile. By studying the quantitative metabolic signatures of cholesterol, we might assume that both cholesterol synthesis and absorption are increased, that is, there is an imbalance in the cholesterol homeostasis regulation in women affected by the disease.",
publisher = "Medycyna Praktyczna Cholerzyn",
journal = "Polish Archives of Internal Medicine",
title = "Cholesterol homeostasis is dysregulated in women with preeclampsia",
volume = "131",
number = "12",
doi = "10.20452/pamw.16144"
}

Antonić, T., Ardalić, D., Vladimirov, S., Banjac, G., Cabunac, P., Zeljković, A., Karadžov-Orlić, N., Spasojević-Kalimanovska, V., Miković, Ž.,& Stefanović, A.. (2021). Cholesterol homeostasis is dysregulated in women with preeclampsia. in Polish Archives of Internal Medicine
Medycyna Praktyczna Cholerzyn., 131(12).
https://doi.org/10.20452/pamw.16144

Antonić T, Ardalić D, Vladimirov S, Banjac G, Cabunac P, Zeljković A, Karadžov-Orlić N, Spasojević-Kalimanovska V, Miković Ž, Stefanović A. Cholesterol homeostasis is dysregulated in women with preeclampsia. in Polish Archives of Internal Medicine. 2021;131(12).
doi:10.20452/pamw.16144 .

Antonić, Tamara, Ardalić, Daniela, Vladimirov, Sandra, Banjac, Gorica, Cabunac, Petar, Zeljković, Aleksandra, Karadžov-Orlić, Natasa, Spasojević-Kalimanovska, Vesna, Miković, Željko, Stefanović, Aleksandra, "Cholesterol homeostasis is dysregulated in women with preeclampsia" in Polish Archives of Internal Medicine, 131, no. 12 (2021),
https://doi.org/10.20452/pamw.16144 . .

TY  - JOUR
AU  - Munjas, Jelena
AU  - Sopić, Miron
AU  - Stefanović, Aleksandra
AU  - Košir, Rok
AU  - Ninić, Ana
AU  - Joksić, Ivana
AU  - Antonić, Tamara
AU  - Spasojević-Kalimanovska, Vesna
AU  - Prosenc Zmrzljak, Uršula
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3979
AB  - Preeclampsia (PE) is a leading cause of maternal and neonatal morbidity and mortality worldwide. Defects in trophoblast invasion, differentiation of extravillous trophoblasts and spiral artery remodeling are key factors in PE development. Currently there are no predictive biomarkers clinically available for PE. Recent technological advancements empowered transcriptome exploration and led to the discovery of numerous non-coding RNA species of which microRNAs (miRNAs) and long non-coding RNAs (lncRNAs) are the most investigated. They are implicated in the regulation of numerous cellular functions, and as such are being extensively explored as potential biomarkers for various diseases. Altered expression of numerous lncRNAs and miRNAs in placenta has been related to pathophysiological processes that occur in preeclampsia. In the following text we offer summary of the latest knowledge of the molecular mechanism by which lnRNAs and miRNAs (focusing on the chromosome 19 miRNA cluster (C19MC)) contribute to pathophysiology of PE development and their potential utility as biomarkers of PE, with special focus on sample selection and techniques for the quantification of lncRNAs and miRNAs in maternal circulation.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - Non-coding RNAs in preeclampsia—molecular mechanisms and diagnostic potential
VL  - 22
IS  - 19
DO  - 10.3390/ijms221910652
ER  - 

@article{
author = "Munjas, Jelena and Sopić, Miron and Stefanović, Aleksandra and Košir, Rok and Ninić, Ana and Joksić, Ivana and Antonić, Tamara and Spasojević-Kalimanovska, Vesna and Prosenc Zmrzljak, Uršula",
year = "2021",
abstract = "Preeclampsia (PE) is a leading cause of maternal and neonatal morbidity and mortality worldwide. Defects in trophoblast invasion, differentiation of extravillous trophoblasts and spiral artery remodeling are key factors in PE development. Currently there are no predictive biomarkers clinically available for PE. Recent technological advancements empowered transcriptome exploration and led to the discovery of numerous non-coding RNA species of which microRNAs (miRNAs) and long non-coding RNAs (lncRNAs) are the most investigated. They are implicated in the regulation of numerous cellular functions, and as such are being extensively explored as potential biomarkers for various diseases. Altered expression of numerous lncRNAs and miRNAs in placenta has been related to pathophysiological processes that occur in preeclampsia. In the following text we offer summary of the latest knowledge of the molecular mechanism by which lnRNAs and miRNAs (focusing on the chromosome 19 miRNA cluster (C19MC)) contribute to pathophysiology of PE development and their potential utility as biomarkers of PE, with special focus on sample selection and techniques for the quantification of lncRNAs and miRNAs in maternal circulation.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "Non-coding RNAs in preeclampsia—molecular mechanisms and diagnostic potential",
volume = "22",
number = "19",
doi = "10.3390/ijms221910652"
}

Munjas, J., Sopić, M., Stefanović, A., Košir, R., Ninić, A., Joksić, I., Antonić, T., Spasojević-Kalimanovska, V.,& Prosenc Zmrzljak, U.. (2021). Non-coding RNAs in preeclampsia—molecular mechanisms and diagnostic potential. in International Journal of Molecular Sciences
MDPI., 22(19).
https://doi.org/10.3390/ijms221910652

Munjas J, Sopić M, Stefanović A, Košir R, Ninić A, Joksić I, Antonić T, Spasojević-Kalimanovska V, Prosenc Zmrzljak U. Non-coding RNAs in preeclampsia—molecular mechanisms and diagnostic potential. in International Journal of Molecular Sciences. 2021;22(19).
doi:10.3390/ijms221910652 .

Munjas, Jelena, Sopić, Miron, Stefanović, Aleksandra, Košir, Rok, Ninić, Ana, Joksić, Ivana, Antonić, Tamara, Spasojević-Kalimanovska, Vesna, Prosenc Zmrzljak, Uršula, "Non-coding RNAs in preeclampsia—molecular mechanisms and diagnostic potential" in International Journal of Molecular Sciences, 22, no. 19 (2021),
https://doi.org/10.3390/ijms221910652 . .

TY  - JOUR
AU  - Milić, Mirta
AU  - Ceppi, Marcello
AU  - Bruzzone, Marco
AU  - Azqueta, Amaya
AU  - Brunborg, Gunnar
AU  - Godschalk, Roger
AU  - Koppen, Gudrun
AU  - Langie, Sabine
AU  - Møller, Peter
AU  - Teixeira, João Paulo
AU  - Alija, Avdulla
AU  - Anderson, Diana
AU  - Andrade, Vanessa
AU  - Andreoli, Cristina
AU  - Asllani, Fisnik
AU  - Bangkoglu, Ezgi Eyluel
AU  - Barančoková, Magdalena
AU  - Basaran, Nursen
AU  - Boutet-Robinet, Elisa
AU  - Buschini, Annamaria
AU  - Cavallo, Delia
AU  - Costa Pereira, Cristiana
AU  - Costa, Carla
AU  - Costa, Solange
AU  - Da Silva, Juliana
AU  - Del Boˊ, Cristian
AU  - Dimitrijević Srećković, Vesna
AU  - Đelić, Ninoslav
AU  - Dobrzyńska, Malgorzata
AU  - Duračková, Zdenka
AU  - Dvořáková, Monika
AU  - Gajski, Goran
AU  - Galati, Serena
AU  - García Lima, Omar
AU  - Giovannelli, Lisa
AU  - Goroshinskaya, Irina A.
AU  - Grindel, Annemarie
AU  - Gutzkow, Kristine B.
AU  - Hernández, Alba
AU  - Hernández, Carlos
AU  - Holven, Kirsten B.
AU  - Ibero-Baraibar, Idoia
AU  - Ottestad, Inger
AU  - Kadioglu, Ela
AU  - Kažimirová, Alena
AU  - Kuznetsova, Elena
AU  - Ladeira, Carina
AU  - Laffon, Blanca
AU  - Lamonaca, Palma
AU  - Lebailly, Pierre
AU  - Louro, Henriqueta
AU  - Mandina Cardoso, Tania
AU  - Marcon, Francesca
AU  - Marcos, Ricard
AU  - Moretti, Massimo
AU  - Moretti, Silvia
AU  - Najafzadeh, Mojgan
AU  - Nemeth, Zsuzsanna
AU  - Neri, Monica
AU  - Novotna, Bozena
AU  - Orlow, Irene
AU  - Paduchova, Zuzana
AU  - Pastor, Susana
AU  - Perdry, Hervé
AU  - Spremo-Potparević, Biljana
AU  - Ramadhani, Dwi
AU  - Riso, Patrizia
AU  - Rohr, Paula
AU  - Rojas, Emilio
AU  - Rossner, Pavel
AU  - Safar, Anna
AU  - Sardas, Semra
AU  - Silva, Maria João
AU  - Sirota, Nikolay
AU  - Smolkova, Bozena
AU  - Staruchova, Marta
AU  - Stetina, Rudolf
AU  - Stopper, Helga
AU  - Surikova, Ekaterina I.
AU  - Ulven, Stine M.
AU  - Ursini, Cinzia Lucia
AU  - Valdiglesias, Vanessa
AU  - Valverde, Mahara
AU  - Vodicka, Pavel
AU  - Volkovova, Katarina
AU  - Wagner, Karl-Heinz
AU  - Živković, Lada
AU  - Dušinská, Maria
AU  - Collins, Andrew R.
AU  - Bonassi, Stefano
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3791
AB  - The alkaline comet assay, or single cell gel electrophoresis, is one of the most popular methods for assessing DNA damage in human population. One of the open issues concerning this assay is the identification of those factors that can explain the large inter-individual and inter-laboratory variation. International collaborative initiatives such as the hCOMET project - a COST Action launched in 2016 - represent a valuable tool to meet this challenge. The aims of hCOMET were to establish reference values for the level of DNA damage in humans, to investigate the effect of host factors, lifestyle and exposure to genotoxic agents, and to compare different sources of assay variability. A database of 19,320 subjects was generated, pooling data from 105 studies run by 44 laboratories in 26 countries between 1999 and 2019. A mixed random effect log-linear model, in parallel with a classic meta-analysis, was applied to take into account the extensive heterogeneity of data, due to descriptor, specimen and protocol variability. As a result of this analysis interquartile intervals of DNA strand breaks (which includes alkali-labile sites) were reported for tail intensity, tail length, and tail moment (comet assay descriptors). A small variation by age was reported in some datasets, suggesting higher DNA damage in oldest age-classes, while no effect could be shown for sex or smoking habit, although the lack of data on heavy smokers has still to be considered. Finally, highly significant differences in DNA damage were found for most exposures investigated in specific studies. In conclusion, these data, which confirm that DNA damage measured by the comet assay is an excellent biomarker of exposure in several conditions, may contribute to improving the quality of study design and to the standardization of results of the comet assay in human populations.
PB  - Elsevier B.V.
T2  - Mutation Research - Reviews in Mutation Research
T1  - The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders
VL  - 787
DO  - 10.1016/j.mrrev.2021.108371
ER  - 

@article{
author = "Milić, Mirta and Ceppi, Marcello and Bruzzone, Marco and Azqueta, Amaya and Brunborg, Gunnar and Godschalk, Roger and Koppen, Gudrun and Langie, Sabine and Møller, Peter and Teixeira, João Paulo and Alija, Avdulla and Anderson, Diana and Andrade, Vanessa and Andreoli, Cristina and Asllani, Fisnik and Bangkoglu, Ezgi Eyluel and Barančoková, Magdalena and Basaran, Nursen and Boutet-Robinet, Elisa and Buschini, Annamaria and Cavallo, Delia and Costa Pereira, Cristiana and Costa, Carla and Costa, Solange and Da Silva, Juliana and Del Boˊ, Cristian and Dimitrijević Srećković, Vesna and Đelić, Ninoslav and Dobrzyńska, Malgorzata and Duračková, Zdenka and Dvořáková, Monika and Gajski, Goran and Galati, Serena and García Lima, Omar and Giovannelli, Lisa and Goroshinskaya, Irina A. and Grindel, Annemarie and Gutzkow, Kristine B. and Hernández, Alba and Hernández, Carlos and Holven, Kirsten B. and Ibero-Baraibar, Idoia and Ottestad, Inger and Kadioglu, Ela and Kažimirová, Alena and Kuznetsova, Elena and Ladeira, Carina and Laffon, Blanca and Lamonaca, Palma and Lebailly, Pierre and Louro, Henriqueta and Mandina Cardoso, Tania and Marcon, Francesca and Marcos, Ricard and Moretti, Massimo and Moretti, Silvia and Najafzadeh, Mojgan and Nemeth, Zsuzsanna and Neri, Monica and Novotna, Bozena and Orlow, Irene and Paduchova, Zuzana and Pastor, Susana and Perdry, Hervé and Spremo-Potparević, Biljana and Ramadhani, Dwi and Riso, Patrizia and Rohr, Paula and Rojas, Emilio and Rossner, Pavel and Safar, Anna and Sardas, Semra and Silva, Maria João and Sirota, Nikolay and Smolkova, Bozena and Staruchova, Marta and Stetina, Rudolf and Stopper, Helga and Surikova, Ekaterina I. and Ulven, Stine M. and Ursini, Cinzia Lucia and Valdiglesias, Vanessa and Valverde, Mahara and Vodicka, Pavel and Volkovova, Katarina and Wagner, Karl-Heinz and Živković, Lada and Dušinská, Maria and Collins, Andrew R. and Bonassi, Stefano",
year = "2021",
abstract = "The alkaline comet assay, or single cell gel electrophoresis, is one of the most popular methods for assessing DNA damage in human population. One of the open issues concerning this assay is the identification of those factors that can explain the large inter-individual and inter-laboratory variation. International collaborative initiatives such as the hCOMET project - a COST Action launched in 2016 - represent a valuable tool to meet this challenge. The aims of hCOMET were to establish reference values for the level of DNA damage in humans, to investigate the effect of host factors, lifestyle and exposure to genotoxic agents, and to compare different sources of assay variability. A database of 19,320 subjects was generated, pooling data from 105 studies run by 44 laboratories in 26 countries between 1999 and 2019. A mixed random effect log-linear model, in parallel with a classic meta-analysis, was applied to take into account the extensive heterogeneity of data, due to descriptor, specimen and protocol variability. As a result of this analysis interquartile intervals of DNA strand breaks (which includes alkali-labile sites) were reported for tail intensity, tail length, and tail moment (comet assay descriptors). A small variation by age was reported in some datasets, suggesting higher DNA damage in oldest age-classes, while no effect could be shown for sex or smoking habit, although the lack of data on heavy smokers has still to be considered. Finally, highly significant differences in DNA damage were found for most exposures investigated in specific studies. In conclusion, these data, which confirm that DNA damage measured by the comet assay is an excellent biomarker of exposure in several conditions, may contribute to improving the quality of study design and to the standardization of results of the comet assay in human populations.",
publisher = "Elsevier B.V.",
journal = "Mutation Research - Reviews in Mutation Research",
title = "The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders",
volume = "787",
doi = "10.1016/j.mrrev.2021.108371"
}

Milić, M., Ceppi, M., Bruzzone, M., Azqueta, A., Brunborg, G., Godschalk, R., Koppen, G., Langie, S., Møller, P., Teixeira, J. P., Alija, A., Anderson, D., Andrade, V., Andreoli, C., Asllani, F., Bangkoglu, E. E., Barančoková, M., Basaran, N., Boutet-Robinet, E., Buschini, A., Cavallo, D., Costa Pereira, C., Costa, C., Costa, S., Da Silva, J., Del Boˊ, C., Dimitrijević Srećković, V., Đelić, N., Dobrzyńska, M., Duračková, Z., Dvořáková, M., Gajski, G., Galati, S., García Lima, O., Giovannelli, L., Goroshinskaya, I. A., Grindel, A., Gutzkow, K. B., Hernández, A., Hernández, C., Holven, K. B., Ibero-Baraibar, I., Ottestad, I., Kadioglu, E., Kažimirová, A., Kuznetsova, E., Ladeira, C., Laffon, B., Lamonaca, P., Lebailly, P., Louro, H., Mandina Cardoso, T., Marcon, F., Marcos, R., Moretti, M., Moretti, S., Najafzadeh, M., Nemeth, Z., Neri, M., Novotna, B., Orlow, I., Paduchova, Z., Pastor, S., Perdry, H., Spremo-Potparević, B., Ramadhani, D., Riso, P., Rohr, P., Rojas, E., Rossner, P., Safar, A., Sardas, S., Silva, M. J., Sirota, N., Smolkova, B., Staruchova, M., Stetina, R., Stopper, H., Surikova, E. I., Ulven, S. M., Ursini, C. L., Valdiglesias, V., Valverde, M., Vodicka, P., Volkovova, K., Wagner, K., Živković, L., Dušinská, M., Collins, A. R.,& Bonassi, S.. (2021). The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders. in Mutation Research - Reviews in Mutation Research
Elsevier B.V.., 787.
https://doi.org/10.1016/j.mrrev.2021.108371

Milić M, Ceppi M, Bruzzone M, Azqueta A, Brunborg G, Godschalk R, Koppen G, Langie S, Møller P, Teixeira JP, Alija A, Anderson D, Andrade V, Andreoli C, Asllani F, Bangkoglu EE, Barančoková M, Basaran N, Boutet-Robinet E, Buschini A, Cavallo D, Costa Pereira C, Costa C, Costa S, Da Silva J, Del Boˊ C, Dimitrijević Srećković V, Đelić N, Dobrzyńska M, Duračková Z, Dvořáková M, Gajski G, Galati S, García Lima O, Giovannelli L, Goroshinskaya IA, Grindel A, Gutzkow KB, Hernández A, Hernández C, Holven KB, Ibero-Baraibar I, Ottestad I, Kadioglu E, Kažimirová A, Kuznetsova E, Ladeira C, Laffon B, Lamonaca P, Lebailly P, Louro H, Mandina Cardoso T, Marcon F, Marcos R, Moretti M, Moretti S, Najafzadeh M, Nemeth Z, Neri M, Novotna B, Orlow I, Paduchova Z, Pastor S, Perdry H, Spremo-Potparević B, Ramadhani D, Riso P, Rohr P, Rojas E, Rossner P, Safar A, Sardas S, Silva MJ, Sirota N, Smolkova B, Staruchova M, Stetina R, Stopper H, Surikova EI, Ulven SM, Ursini CL, Valdiglesias V, Valverde M, Vodicka P, Volkovova K, Wagner K, Živković L, Dušinská M, Collins AR, Bonassi S. The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders. in Mutation Research - Reviews in Mutation Research. 2021;787.
doi:10.1016/j.mrrev.2021.108371 .

Milić, Mirta, Ceppi, Marcello, Bruzzone, Marco, Azqueta, Amaya, Brunborg, Gunnar, Godschalk, Roger, Koppen, Gudrun, Langie, Sabine, Møller, Peter, Teixeira, João Paulo, Alija, Avdulla, Anderson, Diana, Andrade, Vanessa, Andreoli, Cristina, Asllani, Fisnik, Bangkoglu, Ezgi Eyluel, Barančoková, Magdalena, Basaran, Nursen, Boutet-Robinet, Elisa, Buschini, Annamaria, Cavallo, Delia, Costa Pereira, Cristiana, Costa, Carla, Costa, Solange, Da Silva, Juliana, Del Boˊ, Cristian, Dimitrijević Srećković, Vesna, Đelić, Ninoslav, Dobrzyńska, Malgorzata, Duračková, Zdenka, Dvořáková, Monika, Gajski, Goran, Galati, Serena, García Lima, Omar, Giovannelli, Lisa, Goroshinskaya, Irina A., Grindel, Annemarie, Gutzkow, Kristine B., Hernández, Alba, Hernández, Carlos, Holven, Kirsten B., Ibero-Baraibar, Idoia, Ottestad, Inger, Kadioglu, Ela, Kažimirová, Alena, Kuznetsova, Elena, Ladeira, Carina, Laffon, Blanca, Lamonaca, Palma, Lebailly, Pierre, Louro, Henriqueta, Mandina Cardoso, Tania, Marcon, Francesca, Marcos, Ricard, Moretti, Massimo, Moretti, Silvia, Najafzadeh, Mojgan, Nemeth, Zsuzsanna, Neri, Monica, Novotna, Bozena, Orlow, Irene, Paduchova, Zuzana, Pastor, Susana, Perdry, Hervé, Spremo-Potparević, Biljana, Ramadhani, Dwi, Riso, Patrizia, Rohr, Paula, Rojas, Emilio, Rossner, Pavel, Safar, Anna, Sardas, Semra, Silva, Maria João, Sirota, Nikolay, Smolkova, Bozena, Staruchova, Marta, Stetina, Rudolf, Stopper, Helga, Surikova, Ekaterina I., Ulven, Stine M., Ursini, Cinzia Lucia, Valdiglesias, Vanessa, Valverde, Mahara, Vodicka, Pavel, Volkovova, Katarina, Wagner, Karl-Heinz, Živković, Lada, Dušinská, Maria, Collins, Andrew R., Bonassi, Stefano, "The hCOMET project: International database comparison of results with the comet assay in human biomonitoring. Baseline frequency of DNA damage and effect of main confounders" in Mutation Research - Reviews in Mutation Research, 787 (2021),
https://doi.org/10.1016/j.mrrev.2021.108371 . .

TY  - JOUR
AU  - Klisić, Aleksandra
AU  - Kavarić, Nebojša
AU  - Ninić, Ana
PY  - 2021
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4004
AB  - Various studies have reported contradictory results regarding the relationship of total bilirubin and high‑sensitivity C‑reactive protein levels (hsCRP) with diabetes mellitus Type 2 (DM2). Therefore, we aimed to examine which one of them could be more convenient for the estimation of DM2 risk in postmenopausal women. Materials and Methods: A total of 150 healthy postmenopausal women (mean age 57[53–60] years) and 79 postmenopausal women with DM2 (mean age 66 [61–71] years) were enrolled in cross‑sectional study. Examinees were recruited consecutively in the study during their regular check‑up visit in the Primary Health Care Center in Podgorica, Montenegro, in a period from October 2012 to May 2016. Anthropometric measurements, biochemical parameters, and blood pressure were obtained. Multivariable logistic regression analysis was used to find the independent predictors for DM2 development in postmenopausal women. Results: Age, waist circumference, and total bilirubin were the independent predictors for DM2 development in postmenopausal women (odds ratio [OR] =1.224, 95% confidence interval [CI] [1.117–1.341], P < 0.001; OR = 1.137, [95% CI = 1.036–1.215], P < 0.001, and OR = 0.727, [95% CI = 0.611–0.866], P < 0.001, respectively), whereas hsCRP lost its independent predictive role (OR = 1.155, [95% CI = 0.854–1.560], P = 0.349). Conclusion: Unlike hsCRP, total bilirubin independently correlated with DM2 in postmenopausal women.
PB  - Wolters Kluwer Medknow Publications
T2  - Journal of Research in Medical Sciences
T1  - Are total bilirubin and high-sensitivity C-reactive protein independently associated with Type 2 diabetes mellitus in postmenopausal women?
VL  - 26
IS  - 1
DO  - 10.4103/jrms.JRMS_198_18
ER  - 

@article{
author = "Klisić, Aleksandra and Kavarić, Nebojša and Ninić, Ana",
year = "2021, 2021",
abstract = "Various studies have reported contradictory results regarding the relationship of total bilirubin and high‑sensitivity C‑reactive protein levels (hsCRP) with diabetes mellitus Type 2 (DM2). Therefore, we aimed to examine which one of them could be more convenient for the estimation of DM2 risk in postmenopausal women. Materials and Methods: A total of 150 healthy postmenopausal women (mean age 57[53–60] years) and 79 postmenopausal women with DM2 (mean age 66 [61–71] years) were enrolled in cross‑sectional study. Examinees were recruited consecutively in the study during their regular check‑up visit in the Primary Health Care Center in Podgorica, Montenegro, in a period from October 2012 to May 2016. Anthropometric measurements, biochemical parameters, and blood pressure were obtained. Multivariable logistic regression analysis was used to find the independent predictors for DM2 development in postmenopausal women. Results: Age, waist circumference, and total bilirubin were the independent predictors for DM2 development in postmenopausal women (odds ratio [OR] =1.224, 95% confidence interval [CI] [1.117–1.341], P < 0.001; OR = 1.137, [95% CI = 1.036–1.215], P < 0.001, and OR = 0.727, [95% CI = 0.611–0.866], P < 0.001, respectively), whereas hsCRP lost its independent predictive role (OR = 1.155, [95% CI = 0.854–1.560], P = 0.349). Conclusion: Unlike hsCRP, total bilirubin independently correlated with DM2 in postmenopausal women.",
publisher = "Wolters Kluwer Medknow Publications",
journal = "Journal of Research in Medical Sciences",
title = "Are total bilirubin and high-sensitivity C-reactive protein independently associated with Type 2 diabetes mellitus in postmenopausal women?",
volume = "26",
number = "1",
doi = "10.4103/jrms.JRMS_198_18"
}

Klisić, A., Kavarić, N.,& Ninić, A.. (2021). Are total bilirubin and high-sensitivity C-reactive protein independently associated with Type 2 diabetes mellitus in postmenopausal women?. in Journal of Research in Medical Sciences
Wolters Kluwer Medknow Publications., 26(1).
https://doi.org/10.4103/jrms.JRMS_198_18

Klisić A, Kavarić N, Ninić A. Are total bilirubin and high-sensitivity C-reactive protein independently associated with Type 2 diabetes mellitus in postmenopausal women?. in Journal of Research in Medical Sciences. 2021;26(1).
doi:10.4103/jrms.JRMS_198_18 .

Klisić, Aleksandra, Kavarić, Nebojša, Ninić, Ana, "Are total bilirubin and high-sensitivity C-reactive protein independently associated with Type 2 diabetes mellitus in postmenopausal women?" in Journal of Research in Medical Sciences, 26, no. 1 (2021),
https://doi.org/10.4103/jrms.JRMS_198_18 . .

TY  - JOUR
AU  - Vukićević, Petar
AU  - Klisić, Aleksandra
AU  - Nešković, Vojislava
AU  - Babić, Luka
AU  - Mikić, Aleksandar
AU  - Bogavac-Stanojević, Nataša
AU  - Matković, Miloš
AU  - Milićević, Vladimir
AU  - Aleksić, Nemanja
AU  - Kotur-Stevuljević, Jelena
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3942
AB  - Objective. Coronary artery bypass grafting (CABG) represents the significant source of increased oxidative stress (OS). We aimed to follow the OS status parameters (i.e., ischemia-modified albumin (IMA), malondialdehyde (MDA), superoxide anion, prooxidantantioxidant balance (PAB), total oxidant status (TOS), total antioxidant status (TAS), and superoxide-dismutase (SOD)) change through the predefined study times in two different surgical procedures, i.e., cardiopulmonary bypass (CPB) and off-pump coronary artery bypass grafting (OPCAB). Additionally, we aimed to investigate those OS status parameters in specific study times according to SYNTAX score (SS), an established angiographic score for evaluating the extensity and severity of coronary artery disease. Patients and Methods. A total of 107 patients that were planned to undergo CABG were included (i.e., 47 patients in OPCAB and 60 patients in CPB group). Blood samples were taken at 6 time intervals: before surgery (t1), immediately after intervention (t2), 6 h (t3), 24 h (t4), 48 h (t5), and 96 h after termination of the operation (t6). Results. IMA levels were higher in CPB than that in OPCAB baseline and rose in CPB group in t2 point. TOS decreased in both study groups, compared to baseline values, but without statistical significance. Superoxide anion and PAB significantly increased in t3-t6 study times, in both groups. MDA significantly increased only in CPB group in t5 and t6 interval. MDA was significantly higher in CPB group compared to OPCAB in t6 study point. CPB patients had significantly lower TAS compared to OPCAB patients at the beginning and in t2 and t3 study points. They also had significantly lower SOD activities compared to OPCAB, baseline, and in several study points. Moreover, TAS, SOD, and TAS/TOS ratio were significantly lower, whereas PAB and TOS/TAS were significantly higher in patients with high SS compared to corresponding groups. SOD activity, IMA, and TAS level were the best predictors of high SS. Conclusion. CPB patients were in more severe ischemia baseline than OPCAB group and IMA rose in CPB patients immediately after the surgery end, but not later. Also, the antioxidant status was significantly lower, whereas the prooxidant status was significantly higher in patients with high SS compared to corresponding groups. SOD activity, IMA, and TAS level were the best predictors of CAD (as determined with SS), showing that SOD and IMA had very good discriminatory capability towards higher SS status.
PB  - Hindawi Limited
T2  - Oxidative Medicine and Cellular Longevity
T1  - Oxidative Stress in Patients before and after On-Pump and Off-Pump Coronary Artery Bypass Grafting: Relationship with Syntax Score
VL  - 2021
DO  - 10.1155/2021/3315951
ER  - 

@article{
author = "Vukićević, Petar and Klisić, Aleksandra and Nešković, Vojislava and Babić, Luka and Mikić, Aleksandar and Bogavac-Stanojević, Nataša and Matković, Miloš and Milićević, Vladimir and Aleksić, Nemanja and Kotur-Stevuljević, Jelena",
year = "2021",
abstract = "Objective. Coronary artery bypass grafting (CABG) represents the significant source of increased oxidative stress (OS). We aimed to follow the OS status parameters (i.e., ischemia-modified albumin (IMA), malondialdehyde (MDA), superoxide anion, prooxidantantioxidant balance (PAB), total oxidant status (TOS), total antioxidant status (TAS), and superoxide-dismutase (SOD)) change through the predefined study times in two different surgical procedures, i.e., cardiopulmonary bypass (CPB) and off-pump coronary artery bypass grafting (OPCAB). Additionally, we aimed to investigate those OS status parameters in specific study times according to SYNTAX score (SS), an established angiographic score for evaluating the extensity and severity of coronary artery disease. Patients and Methods. A total of 107 patients that were planned to undergo CABG were included (i.e., 47 patients in OPCAB and 60 patients in CPB group). Blood samples were taken at 6 time intervals: before surgery (t1), immediately after intervention (t2), 6 h (t3), 24 h (t4), 48 h (t5), and 96 h after termination of the operation (t6). Results. IMA levels were higher in CPB than that in OPCAB baseline and rose in CPB group in t2 point. TOS decreased in both study groups, compared to baseline values, but without statistical significance. Superoxide anion and PAB significantly increased in t3-t6 study times, in both groups. MDA significantly increased only in CPB group in t5 and t6 interval. MDA was significantly higher in CPB group compared to OPCAB in t6 study point. CPB patients had significantly lower TAS compared to OPCAB patients at the beginning and in t2 and t3 study points. They also had significantly lower SOD activities compared to OPCAB, baseline, and in several study points. Moreover, TAS, SOD, and TAS/TOS ratio were significantly lower, whereas PAB and TOS/TAS were significantly higher in patients with high SS compared to corresponding groups. SOD activity, IMA, and TAS level were the best predictors of high SS. Conclusion. CPB patients were in more severe ischemia baseline than OPCAB group and IMA rose in CPB patients immediately after the surgery end, but not later. Also, the antioxidant status was significantly lower, whereas the prooxidant status was significantly higher in patients with high SS compared to corresponding groups. SOD activity, IMA, and TAS level were the best predictors of CAD (as determined with SS), showing that SOD and IMA had very good discriminatory capability towards higher SS status.",
publisher = "Hindawi Limited",
journal = "Oxidative Medicine and Cellular Longevity",
title = "Oxidative Stress in Patients before and after On-Pump and Off-Pump Coronary Artery Bypass Grafting: Relationship with Syntax Score",
volume = "2021",
doi = "10.1155/2021/3315951"
}

Vukićević, P., Klisić, A., Nešković, V., Babić, L., Mikić, A., Bogavac-Stanojević, N., Matković, M., Milićević, V., Aleksić, N.,& Kotur-Stevuljević, J.. (2021). Oxidative Stress in Patients before and after On-Pump and Off-Pump Coronary Artery Bypass Grafting: Relationship with Syntax Score. in Oxidative Medicine and Cellular Longevity
Hindawi Limited., 2021.
https://doi.org/10.1155/2021/3315951

Vukićević P, Klisić A, Nešković V, Babić L, Mikić A, Bogavac-Stanojević N, Matković M, Milićević V, Aleksić N, Kotur-Stevuljević J. Oxidative Stress in Patients before and after On-Pump and Off-Pump Coronary Artery Bypass Grafting: Relationship with Syntax Score. in Oxidative Medicine and Cellular Longevity. 2021;2021.
doi:10.1155/2021/3315951 .

Vukićević, Petar, Klisić, Aleksandra, Nešković, Vojislava, Babić, Luka, Mikić, Aleksandar, Bogavac-Stanojević, Nataša, Matković, Miloš, Milićević, Vladimir, Aleksić, Nemanja, Kotur-Stevuljević, Jelena, "Oxidative Stress in Patients before and after On-Pump and Off-Pump Coronary Artery Bypass Grafting: Relationship with Syntax Score" in Oxidative Medicine and Cellular Longevity, 2021 (2021),
https://doi.org/10.1155/2021/3315951 . .

TY  - JOUR
AU  - Vukićević, Petar
AU  - Klisić, Aleksandra
AU  - Kotur-Stevuljević, Jelena
AU  - Mikić, Aleksandar
AU  - Bogavac-Stanojević, Nataša
AU  - Matković, Miloš
AU  - Putnik, Svetozar
AU  - Aleksić, Nemanja
AU  - Tutus, Vladimir
AU  - Karikas, Georg Albert
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3788
AB  - OBJECTIVE: Coronary artery bypass grafting (CABG) seems to present a powerful trigger of oxidative stress (OS) and acute inflammatory response. This study aimed to estimate the effects of off-pump coronary artery bypass (OPCAB) grafting on the OS that is commonly observed in patients undergoing operation under cardiopulmonary bypass (CPB). Additionally, we aimed to examine the relationship between and paraoxonase 1 (PON1) activity and the degree of stenosis, severity and complexity of the atherosclerotic lesions, estimated by SYNTAX score (SS). PATIENTS AND METHODS: Study group of 107 patients scheduled for CABG were divided into CPB and OPCAB group. Blood samples for OS markers measurement were collected at six-time intervals: before skin incision (t1), immediately after surgery (t2), 6h (t3), 24h (t4), 48h (t5) and 96h after cessation of the operation and surgical trauma (t6). SS was calculated. RESULTS: A significant decrease in lipid hydroperoxides (LOOH) and advanced oxidation protein products (AOPP) levels after both types of surgeries were observed, whereas PON1 reduction was observed higher in the CPB than in the OPCAB group. A significant inverse correlation between SS values and PON1 activity, preoperatively and during the early postoperative hours after surgery [in t2, t3 time intervals (p<0.05 for all)] was found. ROC analysis showed that for CPB patients, Model with all OS parameters showed excellent accuracy (AUC=0.957, p<0.001) for prediction postoperative complications. CONCLUSIONS: Decrease in PON1 activity during the early post-operative phases was related to higher SS. This relationship was more convincing in CPB, compared with OPCAB patients. Moreover, integrated models of OS status parameters have the capability to predict the development of postoperative complications.
PB  - Verduci Editore s.r.l
T2  - European Review for Medical and Pharmacological Sciences
T1  - Paraoxonase 1 low activity and SYNTAX score may predict postoperative complications after coronary artery surgery
VL  - 25
IS  - 3
SP  - 1511
EP  - 1521
DO  - 10.26355/eurrev_202102_24858
ER  - 

@article{
author = "Vukićević, Petar and Klisić, Aleksandra and Kotur-Stevuljević, Jelena and Mikić, Aleksandar and Bogavac-Stanojević, Nataša and Matković, Miloš and Putnik, Svetozar and Aleksić, Nemanja and Tutus, Vladimir and Karikas, Georg Albert",
year = "2021",
abstract = "OBJECTIVE: Coronary artery bypass grafting (CABG) seems to present a powerful trigger of oxidative stress (OS) and acute inflammatory response. This study aimed to estimate the effects of off-pump coronary artery bypass (OPCAB) grafting on the OS that is commonly observed in patients undergoing operation under cardiopulmonary bypass (CPB). Additionally, we aimed to examine the relationship between and paraoxonase 1 (PON1) activity and the degree of stenosis, severity and complexity of the atherosclerotic lesions, estimated by SYNTAX score (SS). PATIENTS AND METHODS: Study group of 107 patients scheduled for CABG were divided into CPB and OPCAB group. Blood samples for OS markers measurement were collected at six-time intervals: before skin incision (t1), immediately after surgery (t2), 6h (t3), 24h (t4), 48h (t5) and 96h after cessation of the operation and surgical trauma (t6). SS was calculated. RESULTS: A significant decrease in lipid hydroperoxides (LOOH) and advanced oxidation protein products (AOPP) levels after both types of surgeries were observed, whereas PON1 reduction was observed higher in the CPB than in the OPCAB group. A significant inverse correlation between SS values and PON1 activity, preoperatively and during the early postoperative hours after surgery [in t2, t3 time intervals (p<0.05 for all)] was found. ROC analysis showed that for CPB patients, Model with all OS parameters showed excellent accuracy (AUC=0.957, p<0.001) for prediction postoperative complications. CONCLUSIONS: Decrease in PON1 activity during the early post-operative phases was related to higher SS. This relationship was more convincing in CPB, compared with OPCAB patients. Moreover, integrated models of OS status parameters have the capability to predict the development of postoperative complications.",
publisher = "Verduci Editore s.r.l",
journal = "European Review for Medical and Pharmacological Sciences",
title = "Paraoxonase 1 low activity and SYNTAX score may predict postoperative complications after coronary artery surgery",
volume = "25",
number = "3",
pages = "1511-1521",
doi = "10.26355/eurrev_202102_24858"
}

Vukićević, P., Klisić, A., Kotur-Stevuljević, J., Mikić, A., Bogavac-Stanojević, N., Matković, M., Putnik, S., Aleksić, N., Tutus, V.,& Karikas, G. A.. (2021). Paraoxonase 1 low activity and SYNTAX score may predict postoperative complications after coronary artery surgery. in European Review for Medical and Pharmacological Sciences
Verduci Editore s.r.l., 25(3), 1511-1521.
https://doi.org/10.26355/eurrev_202102_24858

Vukićević P, Klisić A, Kotur-Stevuljević J, Mikić A, Bogavac-Stanojević N, Matković M, Putnik S, Aleksić N, Tutus V, Karikas GA. Paraoxonase 1 low activity and SYNTAX score may predict postoperative complications after coronary artery surgery. in European Review for Medical and Pharmacological Sciences. 2021;25(3):1511-1521.
doi:10.26355/eurrev_202102_24858 .

Vukićević, Petar, Klisić, Aleksandra, Kotur-Stevuljević, Jelena, Mikić, Aleksandar, Bogavac-Stanojević, Nataša, Matković, Miloš, Putnik, Svetozar, Aleksić, Nemanja, Tutus, Vladimir, Karikas, Georg Albert, "Paraoxonase 1 low activity and SYNTAX score may predict postoperative complications after coronary artery surgery" in European Review for Medical and Pharmacological Sciences, 25, no. 3 (2021):1511-1521,
https://doi.org/10.26355/eurrev_202102_24858 . .

TY  - JOUR
AU  - Vekić, Jelena
AU  - Kotur-Stevuljević, Jelena
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3350
PB  - Taylor & Francis Inc, Philadelphia
T2  - Journal of Investigative Surgery
T1  - The Potential Importance of Paraoxonase-1 in Surgical Patients
VL  - 34
IS  - 2
SP  - 225
EP  - 226
DO  - 10.1080/08941939.2019.1606964
ER  - 

@article{
author = "Vekić, Jelena and Kotur-Stevuljević, Jelena",
year = "2021",
publisher = "Taylor & Francis Inc, Philadelphia",
journal = "Journal of Investigative Surgery",
title = "The Potential Importance of Paraoxonase-1 in Surgical Patients",
volume = "34",
number = "2",
pages = "225-226",
doi = "10.1080/08941939.2019.1606964"
}

Vekić, J.,& Kotur-Stevuljević, J.. (2021). The Potential Importance of Paraoxonase-1 in Surgical Patients. in Journal of Investigative Surgery
Taylor & Francis Inc, Philadelphia., 34(2), 225-226.
https://doi.org/10.1080/08941939.2019.1606964

Vekić J, Kotur-Stevuljević J. The Potential Importance of Paraoxonase-1 in Surgical Patients. in Journal of Investigative Surgery. 2021;34(2):225-226.
doi:10.1080/08941939.2019.1606964 .

Vekić, Jelena, Kotur-Stevuljević, Jelena, "The Potential Importance of Paraoxonase-1 in Surgical Patients" in Journal of Investigative Surgery, 34, no. 2 (2021):225-226,
https://doi.org/10.1080/08941939.2019.1606964 . .

TY  - THES
AU  - Vuković-Dejanović, Vesna
PY  - 2020
UR  - http://eteze.bg.ac.rs/application/showtheses?thesesId=7913
UR  - https://fedorabg.bg.ac.rs/fedora/get/o:23263/bdef:Content/download
UR  - http://vbs.rs/scripts/cobiss?command=DISPLAY&base=70036&RID=23232521
UR  - https://nardus.mpn.gov.rs/handle/123456789/17840
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4273
AB  - Poznato je da je koronarna arterijska bolest (KAB) povezana sa inflamatornim i oksidativnimprocesima koji dovode do okluzije koronarnih arterija i akutnog koronarnog sindroma, najčešćeinfarkta miokarda sa ST-elevacijom (STEMI). Još uvek se traga za biomarkerima koji bi bili korisni uklasifikaciji bolesnika prema težini bolesti i u predviđanju budućih srčanih događaja. Svrha ove studijeje da se ispitaju koncentracije pentraksina 3 (PTX3), ciklofilina A (CyPA) i heparin-vezujućegepidermalnog faktora rasta (HB-EGF) kod bolesnika sa STEMI (N=87) u odnosu na zdrave osobe(N=193) i bolesnike sa stabilnom anginom pektoris (N=15) kako bi se utvrdilo da li su promene ovihparametara posledica hronične srčane bolesti ili im se vrednosti menjaju usled akutnih poremećaja uSTEMI. Dodatno je analizirano da li primena primarne perkutane koronarne intervencije (pPCI) dovodido oštećenja krvnih sudova srca što će se manifestovati kroz promenu u koncentracijama navedenihispitivanih parametara. Ovi parametri su analizirani i kod bolesnika sa dijagnozom infarkta miokardabez opstrukcije koronarnih arterija (MINOCA). Studija je pokazala da su inflamatorni biomarkeriPTX3 i HB-EGF povišeni u STEMI i da se značajno menjaju posle pPCI kod bolesnika saopstruktivnom KAB u poređenju sa vrednostima pre procedure. MINOCA bolesnici imali su značajnoviše vrednosti PTX3 u odnosu na bolesnike sa STEMI. Kako bi se ispitali nezavisni prediktori visokihkoncentracija PTX3 i HB-EGF kod bolesnika sa STEMI, primenjena je faktorska analiza. Faktormetaboličko-oksidativnog stresa je značajan prediktor visokih koncentracija PTX3, dok je PTX3značajan prediktor visokih koncentracija HB-EGF. Osim toga, rezultati su pokazali da je identifikacijaMINOCA etiologije ključna za lečenje ovih bolesnika u čemu, pored standardnih procedura, možepomoći određivanje koncentracije PTX3.
AB  - Since the association of coronary artery disease (CAD) with inflammatory and oxidativeprocesses that leads to the development of coronary artery occlusion and acute coronary syndrome,most commonly in the form of ST-elevation myocardial infarction (STEMI) have been recognized,biomarkers that would be useful in classifying patients according to disease severity and in predictingfuture cardiac events are still being sought. This study aimed to examine the concentrations ofpentraxin 3 (PTX3), cyclophilin A (CyPA) and heparin-binding epidermal growth factor-like growthfactor (HB-EGF) in patients with STEMI (N = 87) compared to healthy subjects (N = 193) and patientswith stable angina pectoris (N = 15) to determine whether changes in these parameters are due tochronic heart disease or their values change due to acute disorders in STEMI. It was additionallyanalyzed whether the application of primary percutaneous coronary intervention (pPCI) leads todamage of the heart blood vessels, which will be manifested as a change in the concentrations of theexamined parameters. These parameters were also analyzed in patients with the diagnosis of themyocardial infarction without coronary artery obstruction (MINOCA). This study showed that theinflammatory biomarkers PTX3 and HB-EGF are elevated in STEMI and change significantly afterpPCI in patients with obstructive CAD compared to pre-procedure values. MINOCA patients hadsignificantly higher PTX3 values compared to patients with STEMI. Factor analysis was applied toexamine independent predictors of high PTX3 and HB-EGF concentrations in patients with STEMI.The metabolic-oxidative stress factor is a significant predictor of high concentrations of PTX3, whilePTX3 is a significant predictor of high concentrations of HB-EGF. Besides, the results showed that theidentification of MINOCA etiology is crucial for the treatment of these patients in which thedetermination of PTX3 concentration can help, in addition to standard procedures.
PB  - Универзитет у Београду, Фармацеутски факултет
T2  - Универзитет у Београду
T1  - Ispitivanje markera inflamacije pentraksina 3, ciklofilina A i heparin-vezujućeg epidermalnog faktora rasta kod bolesnika sa akutnim infarktom miokarda sa ST-elevacijom
UR  - https://hdl.handle.net/21.15107/rcub_nardus_17840
ER  - 

@phdthesis{
author = "Vuković-Dejanović, Vesna",
year = "2020",
abstract = "Poznato je da je koronarna arterijska bolest (KAB) povezana sa inflamatornim i oksidativnimprocesima koji dovode do okluzije koronarnih arterija i akutnog koronarnog sindroma, najčešćeinfarkta miokarda sa ST-elevacijom (STEMI). Još uvek se traga za biomarkerima koji bi bili korisni uklasifikaciji bolesnika prema težini bolesti i u predviđanju budućih srčanih događaja. Svrha ove studijeje da se ispitaju koncentracije pentraksina 3 (PTX3), ciklofilina A (CyPA) i heparin-vezujućegepidermalnog faktora rasta (HB-EGF) kod bolesnika sa STEMI (N=87) u odnosu na zdrave osobe(N=193) i bolesnike sa stabilnom anginom pektoris (N=15) kako bi se utvrdilo da li su promene ovihparametara posledica hronične srčane bolesti ili im se vrednosti menjaju usled akutnih poremećaja uSTEMI. Dodatno je analizirano da li primena primarne perkutane koronarne intervencije (pPCI) dovodido oštećenja krvnih sudova srca što će se manifestovati kroz promenu u koncentracijama navedenihispitivanih parametara. Ovi parametri su analizirani i kod bolesnika sa dijagnozom infarkta miokardabez opstrukcije koronarnih arterija (MINOCA). Studija je pokazala da su inflamatorni biomarkeriPTX3 i HB-EGF povišeni u STEMI i da se značajno menjaju posle pPCI kod bolesnika saopstruktivnom KAB u poređenju sa vrednostima pre procedure. MINOCA bolesnici imali su značajnoviše vrednosti PTX3 u odnosu na bolesnike sa STEMI. Kako bi se ispitali nezavisni prediktori visokihkoncentracija PTX3 i HB-EGF kod bolesnika sa STEMI, primenjena je faktorska analiza. Faktormetaboličko-oksidativnog stresa je značajan prediktor visokih koncentracija PTX3, dok je PTX3značajan prediktor visokih koncentracija HB-EGF. Osim toga, rezultati su pokazali da je identifikacijaMINOCA etiologije ključna za lečenje ovih bolesnika u čemu, pored standardnih procedura, možepomoći određivanje koncentracije PTX3., Since the association of coronary artery disease (CAD) with inflammatory and oxidativeprocesses that leads to the development of coronary artery occlusion and acute coronary syndrome,most commonly in the form of ST-elevation myocardial infarction (STEMI) have been recognized,biomarkers that would be useful in classifying patients according to disease severity and in predictingfuture cardiac events are still being sought. This study aimed to examine the concentrations ofpentraxin 3 (PTX3), cyclophilin A (CyPA) and heparin-binding epidermal growth factor-like growthfactor (HB-EGF) in patients with STEMI (N = 87) compared to healthy subjects (N = 193) and patientswith stable angina pectoris (N = 15) to determine whether changes in these parameters are due tochronic heart disease or their values change due to acute disorders in STEMI. It was additionallyanalyzed whether the application of primary percutaneous coronary intervention (pPCI) leads todamage of the heart blood vessels, which will be manifested as a change in the concentrations of theexamined parameters. These parameters were also analyzed in patients with the diagnosis of themyocardial infarction without coronary artery obstruction (MINOCA). This study showed that theinflammatory biomarkers PTX3 and HB-EGF are elevated in STEMI and change significantly afterpPCI in patients with obstructive CAD compared to pre-procedure values. MINOCA patients hadsignificantly higher PTX3 values compared to patients with STEMI. Factor analysis was applied toexamine independent predictors of high PTX3 and HB-EGF concentrations in patients with STEMI.The metabolic-oxidative stress factor is a significant predictor of high concentrations of PTX3, whilePTX3 is a significant predictor of high concentrations of HB-EGF. Besides, the results showed that theidentification of MINOCA etiology is crucial for the treatment of these patients in which thedetermination of PTX3 concentration can help, in addition to standard procedures.",
publisher = "Универзитет у Београду, Фармацеутски факултет",
journal = "Универзитет у Београду",
title = "Ispitivanje markera inflamacije pentraksina 3, ciklofilina A i heparin-vezujućeg epidermalnog faktora rasta kod bolesnika sa akutnim infarktom miokarda sa ST-elevacijom",
url = "https://hdl.handle.net/21.15107/rcub_nardus_17840"
}

Vuković-Dejanović, V.. (2020). Ispitivanje markera inflamacije pentraksina 3, ciklofilina A i heparin-vezujućeg epidermalnog faktora rasta kod bolesnika sa akutnim infarktom miokarda sa ST-elevacijom. in Универзитет у Београду
Универзитет у Београду, Фармацеутски факултет..
https://hdl.handle.net/21.15107/rcub_nardus_17840

Vuković-Dejanović V. Ispitivanje markera inflamacije pentraksina 3, ciklofilina A i heparin-vezujućeg epidermalnog faktora rasta kod bolesnika sa akutnim infarktom miokarda sa ST-elevacijom. in Универзитет у Београду. 2020;.
https://hdl.handle.net/21.15107/rcub_nardus_17840 .

Vuković-Dejanović, Vesna, "Ispitivanje markera inflamacije pentraksina 3, ciklofilina A i heparin-vezujućeg epidermalnog faktora rasta kod bolesnika sa akutnim infarktom miokarda sa ST-elevacijom" in Универзитет у Београду (2020),
https://hdl.handle.net/21.15107/rcub_nardus_17840 .

TY  - JOUR
AU  - Milinković, Neda
AU  - Sarić, Marija
AU  - Jovičić, Snežana
AU  - Mirković, Duško
AU  - Ležaić, Višnja
AU  - Ignjatović, Svetlana
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3624
AB  - Background:Some observational studies indicate an association  of  25-hydroxy  vitamin  D  (25(OH)D)  insufficiency and  atherogenic  cholesterol  concentrations.  The  aim  of this study was to investigate relationship between 25(OH)D concentrations  and  lipid  parameters  in  end  stage  renal disease  (ESRD)  patients,  separately  for  predialysis,  hemodialysis and peritoneal dialysis patients.Methods:We  have  adjusted  25(OH)D  concentrations  for seasonal variability with cosinor analysis, and performed all further analysis using these corrected 25(OH)D concentrations. Concentrations of 25(OH)D and the lipid parameters were  determined  in  214  ESRD  patients  and  50  control group participants. The analysis included the measurement of 25(OH)D by HPLC, apolipoprotein (Apo) AI, ApoB andLp(a) by nephelometry, total cholesterol (TC), high-densitylipoprotein  cholesterol  (HDL-C),  low-density  lipoprotein cholesterol  (LDL-C)  and  triglyceride  (TG)  by  spectrophotometry   and   manually   calculated   ApoB/ApoAI   and LDL-C/HDL-C ratio.Results:ESRD patients with adjusted 25(OH)D concentrations  of  ≤ 50  nmol/L  had  significantly  higher  TC  (P  =0.005) and ApoAI (P = 0.049). Significantly higher HDL-C  (P  =  0.011)  and  ApoAI  (P  =  0.020)  were  found  in hemodialysis patients with the 25(OH)D concentrations of ≤ 50 nmol/L. The other analyzed lipid parameters differed significantly  between  predialysis,  hemodialysis  and  peritoneal dialysis patients with 25(OH)D concentrations of <50 nmol/L. Conclusions:Our study indicate the significant relationship between 25(OH)D repletion and optimal concentrations of lipid parameters in ESRD patients. Further research is necessary  to  explain  whether  joint  evaluation  of  vitamin  D status and lipid abnormalities could improve cardiovascular outcome in ESRD patients.
AB  - Uvod: Opservacione studije ukazuju na povezanost nedostatka 25-hidroksi vitamin D (25(OH)D) i koncentracija aterogenog holesterola. Cilj ove studije bio je da se ispita međusobna povezanost 25(OH)D i lipidnih parametara kod bolesnika sa krajnjim stadijumom bolesti bubrega (ESRD) posebno kod predijaliznih, bolesnika na hemodijalizi i peritonalnoj dijalizi. Metode: Podesili smo koncentracije 25(OH)D za sezonsku varijaciju koristeći kosinor analizu, i u daljem ispitivanju smo koristili korigovane koncentracije 25(OH)D. Koncentracije 25(OH)D i lipidnih parametara su određene za 214 ESRD bolesnika i 50 učesnika kontrolne grupe. Analizirali smo koncentracije 25(OH)D metodom HPLC, apolipoproteina (Apo) AI, ApoB i Lp(a) nefelometrijski, ukupnog holesterola (TC), lipoproteina velike gustine (HDL-C), lipoproteina male gustine (LDL-C) i triglicerida (TG) spektro fotometrijski i ručno preračunavali indekse ApoB/ApoAI i LDL-C/HDLC. Rezultati: ESRD bolesnici sa korigovanim 25(OH)D 50 nmol/L su imali značajno viši TC (P = 0,005) i ApoAI (P = 0,049). Značajno više koncentracije HDL-C (P = 0,011) i ApoAI (P = 0,020) smo dobili kod hemodijaliznih bolesnika sa 25(OH)D koncentracijama 50 nmol/L. Ostali analizirani lipidni parametri su bili značajno različiti između analiziranih grupa bolesnika sa 25(OH)D koncentracijama < 50 nmol/L. Zaključak: Naša studija ukazuje na značajnu povezanost dovoljnih nivoa 25(OH)D i optimalnih koncentracija lipidnih parametara kod ESRD bolesnika. Potrebna su dalja istraživanja da bi se objasnilo da li zajednička procena statusa vitamina D i lipidnih abnormalnosti mogu da poboljšaju kardiovaskularni ishod kod ESRD bolesnika.
PB  - Beograd : Društvo medicinskih biohemičara Srbije
T2  - Journal of Medical Biochemistry
T1  - Lipid status association with 25-hydroxy vitamin D: cross sectional study of end stage renal disease patients
T1  - Povezanost lipidnog statusa sa 25-hidroksi vitaminom D: studija preseka kod bolesnika sa krajnjim stadijumom bolesti bubrega
VL  - 39
IS  - 3
SP  - 309
EP  - 317
DO  - 10.2478/jomb-2019-0032
ER  - 

@article{
author = "Milinković, Neda and Sarić, Marija and Jovičić, Snežana and Mirković, Duško and Ležaić, Višnja and Ignjatović, Svetlana",
year = "2020",
abstract = "Background:Some observational studies indicate an association  of  25-hydroxy  vitamin  D  (25(OH)D)  insufficiency and  atherogenic  cholesterol  concentrations.  The  aim  of this study was to investigate relationship between 25(OH)D concentrations  and  lipid  parameters  in  end  stage  renal disease  (ESRD)  patients,  separately  for  predialysis,  hemodialysis and peritoneal dialysis patients.Methods:We  have  adjusted  25(OH)D  concentrations  for seasonal variability with cosinor analysis, and performed all further analysis using these corrected 25(OH)D concentrations. Concentrations of 25(OH)D and the lipid parameters were  determined  in  214  ESRD  patients  and  50  control group participants. The analysis included the measurement of 25(OH)D by HPLC, apolipoprotein (Apo) AI, ApoB andLp(a) by nephelometry, total cholesterol (TC), high-densitylipoprotein  cholesterol  (HDL-C),  low-density  lipoprotein cholesterol  (LDL-C)  and  triglyceride  (TG)  by  spectrophotometry   and   manually   calculated   ApoB/ApoAI   and LDL-C/HDL-C ratio.Results:ESRD patients with adjusted 25(OH)D concentrations  of  ≤ 50  nmol/L  had  significantly  higher  TC  (P  =0.005) and ApoAI (P = 0.049). Significantly higher HDL-C  (P  =  0.011)  and  ApoAI  (P  =  0.020)  were  found  in hemodialysis patients with the 25(OH)D concentrations of ≤ 50 nmol/L. The other analyzed lipid parameters differed significantly  between  predialysis,  hemodialysis  and  peritoneal dialysis patients with 25(OH)D concentrations of <50 nmol/L. Conclusions:Our study indicate the significant relationship between 25(OH)D repletion and optimal concentrations of lipid parameters in ESRD patients. Further research is necessary  to  explain  whether  joint  evaluation  of  vitamin  D status and lipid abnormalities could improve cardiovascular outcome in ESRD patients., Uvod: Opservacione studije ukazuju na povezanost nedostatka 25-hidroksi vitamin D (25(OH)D) i koncentracija aterogenog holesterola. Cilj ove studije bio je da se ispita međusobna povezanost 25(OH)D i lipidnih parametara kod bolesnika sa krajnjim stadijumom bolesti bubrega (ESRD) posebno kod predijaliznih, bolesnika na hemodijalizi i peritonalnoj dijalizi. Metode: Podesili smo koncentracije 25(OH)D za sezonsku varijaciju koristeći kosinor analizu, i u daljem ispitivanju smo koristili korigovane koncentracije 25(OH)D. Koncentracije 25(OH)D i lipidnih parametara su određene za 214 ESRD bolesnika i 50 učesnika kontrolne grupe. Analizirali smo koncentracije 25(OH)D metodom HPLC, apolipoproteina (Apo) AI, ApoB i Lp(a) nefelometrijski, ukupnog holesterola (TC), lipoproteina velike gustine (HDL-C), lipoproteina male gustine (LDL-C) i triglicerida (TG) spektro fotometrijski i ručno preračunavali indekse ApoB/ApoAI i LDL-C/HDLC. Rezultati: ESRD bolesnici sa korigovanim 25(OH)D 50 nmol/L su imali značajno viši TC (P = 0,005) i ApoAI (P = 0,049). Značajno više koncentracije HDL-C (P = 0,011) i ApoAI (P = 0,020) smo dobili kod hemodijaliznih bolesnika sa 25(OH)D koncentracijama 50 nmol/L. Ostali analizirani lipidni parametri su bili značajno različiti između analiziranih grupa bolesnika sa 25(OH)D koncentracijama < 50 nmol/L. Zaključak: Naša studija ukazuje na značajnu povezanost dovoljnih nivoa 25(OH)D i optimalnih koncentracija lipidnih parametara kod ESRD bolesnika. Potrebna su dalja istraživanja da bi se objasnilo da li zajednička procena statusa vitamina D i lipidnih abnormalnosti mogu da poboljšaju kardiovaskularni ishod kod ESRD bolesnika.",
publisher = "Beograd : Društvo medicinskih biohemičara Srbije",
journal = "Journal of Medical Biochemistry",
title = "Lipid status association with 25-hydroxy vitamin D: cross sectional study of end stage renal disease patients, Povezanost lipidnog statusa sa 25-hidroksi vitaminom D: studija preseka kod bolesnika sa krajnjim stadijumom bolesti bubrega",
volume = "39",
number = "3",
pages = "309-317",
doi = "10.2478/jomb-2019-0032"
}

Milinković, N., Sarić, M., Jovičić, S., Mirković, D., Ležaić, V.,& Ignjatović, S.. (2020). Lipid status association with 25-hydroxy vitamin D: cross sectional study of end stage renal disease patients. in Journal of Medical Biochemistry
Beograd : Društvo medicinskih biohemičara Srbije., 39(3), 309-317.
https://doi.org/10.2478/jomb-2019-0032

Milinković N, Sarić M, Jovičić S, Mirković D, Ležaić V, Ignjatović S. Lipid status association with 25-hydroxy vitamin D: cross sectional study of end stage renal disease patients. in Journal of Medical Biochemistry. 2020;39(3):309-317.
doi:10.2478/jomb-2019-0032 .

Milinković, Neda, Sarić, Marija, Jovičić, Snežana, Mirković, Duško, Ležaić, Višnja, Ignjatović, Svetlana, "Lipid status association with 25-hydroxy vitamin D: cross sectional study of end stage renal disease patients" in Journal of Medical Biochemistry, 39, no. 3 (2020):309-317,
https://doi.org/10.2478/jomb-2019-0032 . .

TY  - JOUR
AU  - Milanović, Zorana
AU  - Beletić, Anđelo
AU  - Vekić, Jelena
AU  - Zeljković, Aleksandra
AU  - Andrić, Nenad
AU  - Ilić Božović, Anja
AU  - Spariosu, Kristina
AU  - Radaković, Milena
AU  - Ajtić, Jelena
AU  - Kovačević Filipović, Milica
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3588
AB  - Asymptomatic outdoor dogs can be carriers of Babesia canis, but data describing the development of an acute phase response (APR) are not available. We hypothesised that these dogs have a moderate APR that could be detected by hematological and biochemical changes. Two groups of Babesia-exposed dogs were represented by nine B. canis PCR-positive and twenty B. canis PCR-negative, seroreactive dogs. The control group consisted of ten Babesia-naïve dogs. Serum amyloid A (SAA), paraoxonase-1 (PON-1), complete blood count, and biochemistry parameters were analysed by standard methodologies. Protein and lipoprotein fractions were separated using agarose gel electrophoresis (GE), and the dominant diameters of lipoproteins were assessed on gradient GE. Results were evaluated using non-parametric tests and the Receiver Operating Characteristic curve. SAA (median 39.0 μg/mL, range 2.2–48.8 μg/mL), total protein (median 74.7 g/L, range 57.1–98.3 g/L) and the dominant diameter of α-lipoproteins (median 13.31 nm, range 12.09–14.17 nm) in B. canis PCR-positive dogs were higher relative to dogs in the control group or dogs that were PCR-negative but seroreactive (p < 0.001 for both groups). Mild to moderate anemia (4/29), thrombocytopenia (7/29), and leukocyte counts that were close to the upper limit of the reference range were encountered in both Babesia-exposed groups. When compared to controls, Babesia-exposed dogs displayed decreased a PON-1 activity and protein GE pattern consistent with low-grade chronic inflammation (p < 0.001 for both groups). Dogs with detectable amounts of B. canis DNA in blood contain increased levels of SAA and total protein along with α-lipoproteins that display an increased diameter relative to those dogs with positive Babesia serology but undetectable levels of B. canis DNA in blood.
PB  - Elsevier
T2  - Veterinary Parasitology
T1  - Evidence of acute phase reaction in asymptomatic dogs naturally infected with Babesia canis
VL  - 282
DO  - 10.1016/j.vetpar.2020.109140
ER  - 

@article{
author = "Milanović, Zorana and Beletić, Anđelo and Vekić, Jelena and Zeljković, Aleksandra and Andrić, Nenad and Ilić Božović, Anja and Spariosu, Kristina and Radaković, Milena and Ajtić, Jelena and Kovačević Filipović, Milica",
year = "2020",
abstract = "Asymptomatic outdoor dogs can be carriers of Babesia canis, but data describing the development of an acute phase response (APR) are not available. We hypothesised that these dogs have a moderate APR that could be detected by hematological and biochemical changes. Two groups of Babesia-exposed dogs were represented by nine B. canis PCR-positive and twenty B. canis PCR-negative, seroreactive dogs. The control group consisted of ten Babesia-naïve dogs. Serum amyloid A (SAA), paraoxonase-1 (PON-1), complete blood count, and biochemistry parameters were analysed by standard methodologies. Protein and lipoprotein fractions were separated using agarose gel electrophoresis (GE), and the dominant diameters of lipoproteins were assessed on gradient GE. Results were evaluated using non-parametric tests and the Receiver Operating Characteristic curve. SAA (median 39.0 μg/mL, range 2.2–48.8 μg/mL), total protein (median 74.7 g/L, range 57.1–98.3 g/L) and the dominant diameter of α-lipoproteins (median 13.31 nm, range 12.09–14.17 nm) in B. canis PCR-positive dogs were higher relative to dogs in the control group or dogs that were PCR-negative but seroreactive (p < 0.001 for both groups). Mild to moderate anemia (4/29), thrombocytopenia (7/29), and leukocyte counts that were close to the upper limit of the reference range were encountered in both Babesia-exposed groups. When compared to controls, Babesia-exposed dogs displayed decreased a PON-1 activity and protein GE pattern consistent with low-grade chronic inflammation (p < 0.001 for both groups). Dogs with detectable amounts of B. canis DNA in blood contain increased levels of SAA and total protein along with α-lipoproteins that display an increased diameter relative to those dogs with positive Babesia serology but undetectable levels of B. canis DNA in blood.",
publisher = "Elsevier",
journal = "Veterinary Parasitology",
title = "Evidence of acute phase reaction in asymptomatic dogs naturally infected with Babesia canis",
volume = "282",
doi = "10.1016/j.vetpar.2020.109140"
}

Milanović, Z., Beletić, A., Vekić, J., Zeljković, A., Andrić, N., Ilić Božović, A., Spariosu, K., Radaković, M., Ajtić, J.,& Kovačević Filipović, M.. (2020). Evidence of acute phase reaction in asymptomatic dogs naturally infected with Babesia canis. in Veterinary Parasitology
Elsevier., 282.
https://doi.org/10.1016/j.vetpar.2020.109140

Milanović Z, Beletić A, Vekić J, Zeljković A, Andrić N, Ilić Božović A, Spariosu K, Radaković M, Ajtić J, Kovačević Filipović M. Evidence of acute phase reaction in asymptomatic dogs naturally infected with Babesia canis. in Veterinary Parasitology. 2020;282.
doi:10.1016/j.vetpar.2020.109140 .

Milanović, Zorana, Beletić, Anđelo, Vekić, Jelena, Zeljković, Aleksandra, Andrić, Nenad, Ilić Božović, Anja, Spariosu, Kristina, Radaković, Milena, Ajtić, Jelena, Kovačević Filipović, Milica, "Evidence of acute phase reaction in asymptomatic dogs naturally infected with Babesia canis" in Veterinary Parasitology, 282 (2020),
https://doi.org/10.1016/j.vetpar.2020.109140 . .

TY  - JOUR
AU  - Munjas, Jelena
AU  - Sopić, Miron
AU  - Joksić, Ivana
AU  - Prosenc Zmrzljak, Ursula
AU  - Karadžov-Orlić, Nataša
AU  - Košir, Rok
AU  - Egić, Amira
AU  - Miković, Željko
AU  - Ninić, Ana
AU  - Spasojević-Kalimanovska, Vesna
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3669
PB  - Elsevier
T2  - Clinical Biochemistry
T1  - Corrigendum to “Placenta-specific plasma miR518b is a potential biomarker for preeclampsia” [Clin. Biochem. 79 (2020) 28–33]
VL  - 85
SP  - 57
EP  - 57
DO  - 10.1016/j.clinbiochem.2020.08.011
ER  - 

@article{
author = "Munjas, Jelena and Sopić, Miron and Joksić, Ivana and Prosenc Zmrzljak, Ursula and Karadžov-Orlić, Nataša and Košir, Rok and Egić, Amira and Miković, Željko and Ninić, Ana and Spasojević-Kalimanovska, Vesna",
year = "2020",
publisher = "Elsevier",
journal = "Clinical Biochemistry",
title = "Corrigendum to “Placenta-specific plasma miR518b is a potential biomarker for preeclampsia” [Clin. Biochem. 79 (2020) 28–33]",
volume = "85",
pages = "57-57",
doi = "10.1016/j.clinbiochem.2020.08.011"
}

Munjas, J., Sopić, M., Joksić, I., Prosenc Zmrzljak, U., Karadžov-Orlić, N., Košir, R., Egić, A., Miković, Ž., Ninić, A.,& Spasojević-Kalimanovska, V.. (2020). Corrigendum to “Placenta-specific plasma miR518b is a potential biomarker for preeclampsia” [Clin. Biochem. 79 (2020) 28–33]. in Clinical Biochemistry
Elsevier., 85, 57-57.
https://doi.org/10.1016/j.clinbiochem.2020.08.011

Munjas J, Sopić M, Joksić I, Prosenc Zmrzljak U, Karadžov-Orlić N, Košir R, Egić A, Miković Ž, Ninić A, Spasojević-Kalimanovska V. Corrigendum to “Placenta-specific plasma miR518b is a potential biomarker for preeclampsia” [Clin. Biochem. 79 (2020) 28–33]. in Clinical Biochemistry. 2020;85:57-57.
doi:10.1016/j.clinbiochem.2020.08.011 .

Munjas, Jelena, Sopić, Miron, Joksić, Ivana, Prosenc Zmrzljak, Ursula, Karadžov-Orlić, Nataša, Košir, Rok, Egić, Amira, Miković, Željko, Ninić, Ana, Spasojević-Kalimanovska, Vesna, "Corrigendum to “Placenta-specific plasma miR518b is a potential biomarker for preeclampsia” [Clin. Biochem. 79 (2020) 28–33]" in Clinical Biochemistry, 85 (2020):57-57,
https://doi.org/10.1016/j.clinbiochem.2020.08.011 . .

TY  - JOUR
AU  - Ardalić, Daniela
AU  - Stefanović, Aleksandra
AU  - Banjac, Gorica
AU  - Cabunac, Petar
AU  - Miljković, Milica
AU  - Mandić-Marković, Vesna
AU  - Stanimirović, Srđan
AU  - Damnjanović Pažin, Barbara
AU  - Spasić, Slavíca
AU  - Spasojević-Kalimanovska, Vesna
AU  - Karadžov-Orlić, Nataša
AU  - Miković, Željko
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3626
AB  - Objectives: The goal of this study was to investigate metabolic changes in lipids and oxidative stress parameters in the first trimester of pregnancy with the more specific aim of estimating the significance and strength of researched parameters in the prediction of preeclampsia. Design and Methods. The study included 87 high-risk pregnant (HRG) female subjects, 14 with developed preeclampsia (PEC) and 43 healthy pregnant female subjects matched for gestational age (CG). Thiobarbituric acid-reactive substances (TBARS) concentration, lipid hydroperoxides (LOOH), pro-oxidant antioxidant balance (PAB) and total oxidative status (TOS) were measured as oxidative stress markers, while total antioxidant capacity (TAC) was measured as an antioxidative defense parameter. The Atherogenic Index of Plasma (AIP) was calculated as the base 10 logarithm of the ratio of the plasma concentration of triglycerides (TG) to the plasma concentration of highdensity lipoprotein cholesterol (HDL-C), with each concentration expressed in mmol/L. Results: The results have shown that lipid indices, especially AIP, were significantly higher in the first trimester of HRG (p < 0.001) and PEC (p < 0.001). Oxidative stress parameters were significantlly higher, while TAC was significantly lower in HRG vs. CG [0.7 ± 0.15 vs 1.1 ± 0.16; (p < 0.001)] and in PEC [0.6 ± 0.12 vs 1.1 ± 0.16; (p < 0.001)] vs. CG. Also, in the HRG, results have shown an independent association of AIP with the preeclampsia development (p < 0.05), while placental growth factor did not show the expected level of significance (p = 0.648). Analysis of the Receiver Operating Characteristics (ROC) curves indicated that certain parameters included in the research model have very good diagnostic accuracy for preeclampsia (AUC = 0.856). Conclusions: AIP is associated with high-risk pregnancies. Furthermore, our results firmly underscored AIP as a potential marker for preeclampsia prediction.
PB  - Elsevier
T2  - Clinical Biochemistry
T1  - Lipid profile and lipid oxidative modification parameters in the first trimester of high- risk pregnancies - possibilities for preeclampsia prediction
VL  - 81
SP  - 34
EP  - 40
DO  - 10.1016/j.clinbiochem.2020.05.003
ER  - 

@article{
author = "Ardalić, Daniela and Stefanović, Aleksandra and Banjac, Gorica and Cabunac, Petar and Miljković, Milica and Mandić-Marković, Vesna and Stanimirović, Srđan and Damnjanović Pažin, Barbara and Spasić, Slavíca and Spasojević-Kalimanovska, Vesna and Karadžov-Orlić, Nataša and Miković, Željko",
year = "2020",
abstract = "Objectives: The goal of this study was to investigate metabolic changes in lipids and oxidative stress parameters in the first trimester of pregnancy with the more specific aim of estimating the significance and strength of researched parameters in the prediction of preeclampsia. Design and Methods. The study included 87 high-risk pregnant (HRG) female subjects, 14 with developed preeclampsia (PEC) and 43 healthy pregnant female subjects matched for gestational age (CG). Thiobarbituric acid-reactive substances (TBARS) concentration, lipid hydroperoxides (LOOH), pro-oxidant antioxidant balance (PAB) and total oxidative status (TOS) were measured as oxidative stress markers, while total antioxidant capacity (TAC) was measured as an antioxidative defense parameter. The Atherogenic Index of Plasma (AIP) was calculated as the base 10 logarithm of the ratio of the plasma concentration of triglycerides (TG) to the plasma concentration of highdensity lipoprotein cholesterol (HDL-C), with each concentration expressed in mmol/L. Results: The results have shown that lipid indices, especially AIP, were significantly higher in the first trimester of HRG (p < 0.001) and PEC (p < 0.001). Oxidative stress parameters were significantlly higher, while TAC was significantly lower in HRG vs. CG [0.7 ± 0.15 vs 1.1 ± 0.16; (p < 0.001)] and in PEC [0.6 ± 0.12 vs 1.1 ± 0.16; (p < 0.001)] vs. CG. Also, in the HRG, results have shown an independent association of AIP with the preeclampsia development (p < 0.05), while placental growth factor did not show the expected level of significance (p = 0.648). Analysis of the Receiver Operating Characteristics (ROC) curves indicated that certain parameters included in the research model have very good diagnostic accuracy for preeclampsia (AUC = 0.856). Conclusions: AIP is associated with high-risk pregnancies. Furthermore, our results firmly underscored AIP as a potential marker for preeclampsia prediction.",
publisher = "Elsevier",
journal = "Clinical Biochemistry",
title = "Lipid profile and lipid oxidative modification parameters in the first trimester of high- risk pregnancies - possibilities for preeclampsia prediction",
volume = "81",
pages = "34-40",
doi = "10.1016/j.clinbiochem.2020.05.003"
}

Ardalić, D., Stefanović, A., Banjac, G., Cabunac, P., Miljković, M., Mandić-Marković, V., Stanimirović, S., Damnjanović Pažin, B., Spasić, S., Spasojević-Kalimanovska, V., Karadžov-Orlić, N.,& Miković, Ž.. (2020). Lipid profile and lipid oxidative modification parameters in the first trimester of high- risk pregnancies - possibilities for preeclampsia prediction. in Clinical Biochemistry
Elsevier., 81, 34-40.
https://doi.org/10.1016/j.clinbiochem.2020.05.003

Ardalić D, Stefanović A, Banjac G, Cabunac P, Miljković M, Mandić-Marković V, Stanimirović S, Damnjanović Pažin B, Spasić S, Spasojević-Kalimanovska V, Karadžov-Orlić N, Miković Ž. Lipid profile and lipid oxidative modification parameters in the first trimester of high- risk pregnancies - possibilities for preeclampsia prediction. in Clinical Biochemistry. 2020;81:34-40.
doi:10.1016/j.clinbiochem.2020.05.003 .

Ardalić, Daniela, Stefanović, Aleksandra, Banjac, Gorica, Cabunac, Petar, Miljković, Milica, Mandić-Marković, Vesna, Stanimirović, Srđan, Damnjanović Pažin, Barbara, Spasić, Slavíca, Spasojević-Kalimanovska, Vesna, Karadžov-Orlić, Nataša, Miković, Željko, "Lipid profile and lipid oxidative modification parameters in the first trimester of high- risk pregnancies - possibilities for preeclampsia prediction" in Clinical Biochemistry, 81 (2020):34-40,
https://doi.org/10.1016/j.clinbiochem.2020.05.003 . .

TY  - JOUR
AU  - Munjas, Jelena
AU  - Sopić, Miron
AU  - Joksić, Ivana
AU  - Prosenc Zmrzljak, Ursula
AU  - Karadžov-Orlić, Nataša
AU  - Košir, Rok
AU  - Egić, Amira
AU  - Miković, Željko
AU  - Ninić, Ana
AU  - Spasojević-Kalimanovska, Vesna
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3602
AB  - Introduction: MicroRNAs have a significant role in the pathogenesis of preeclampsia. Circulating microRNAs could represent a potential biomarker for preeclampsia. The aim of this study was to evaluate plasma miR210-3p and miR518b in preeclampsia and healthy pregnancy for the first time by digital droplet PCR (ddPCR). Methods: Thirty-six pregnant women (seventeen healthy pregnancies, nineteen preeclampsia patients) were involved from the Clinic for Gynaecology and Obstetrics “Narodni front” in Belgrade, Serbia. Plasma miR210-3p, miR518b and cel-miR-39 as a spike-in control were measured by ddPCR. Results: MiR518b was significantly elevated in preeclampsia compared to a healthy pregnancy (P = 0.034; 0.302(0.217–0.421) vs. 0.171(0.110–0.266)). MiR210-3p showed no significant difference between the two groups (P = 0.951). The adjustment of miR518b was made for a gestational age and smoking status and the difference between the preeclampsia and healthy pregnancy group was more significant (P = 0.026; 0.300(0.216–0.419) vs. 0.172(0.121–0.245)). Plasma miR-518b was significantly higher in the group of preeclampsia patients with proteinuria above the 75th percentile for the group (P=0.033), in women who smoked (P=0.039), and was positively related to uric acid in preeclampsia (P = 0.018, r = 0.536). Plasma miR518b was able to significantly discriminate between preeclampsia and healthy pregnancy, yielding AUC of 0.712 (95%CI:0.539–0.891), P = 0.028. Conclusions: In this study plasma microRNA were measured for the first time in preeclampsia and healthy pregnancies with ddPCR. Placenta-specific miR-518b could serve as a potential biomarker for discriminating preeclampsia and healthy pregnancy, which should be confirmed on a larger study population. This study has failed to confirm the same potential for miR210-3p.
PB  - Elsevier
T2  - Clinical Biochemistry
T1  - Placenta-specific plasma miR518b is a potential biomarker for preeclampsia
VL  - 79
SP  - 28
EP  - 33
DO  - 10.1016/j.clinbiochem.2020.02.012
ER  - 

@article{
author = "Munjas, Jelena and Sopić, Miron and Joksić, Ivana and Prosenc Zmrzljak, Ursula and Karadžov-Orlić, Nataša and Košir, Rok and Egić, Amira and Miković, Željko and Ninić, Ana and Spasojević-Kalimanovska, Vesna",
year = "2020",
abstract = "Introduction: MicroRNAs have a significant role in the pathogenesis of preeclampsia. Circulating microRNAs could represent a potential biomarker for preeclampsia. The aim of this study was to evaluate plasma miR210-3p and miR518b in preeclampsia and healthy pregnancy for the first time by digital droplet PCR (ddPCR). Methods: Thirty-six pregnant women (seventeen healthy pregnancies, nineteen preeclampsia patients) were involved from the Clinic for Gynaecology and Obstetrics “Narodni front” in Belgrade, Serbia. Plasma miR210-3p, miR518b and cel-miR-39 as a spike-in control were measured by ddPCR. Results: MiR518b was significantly elevated in preeclampsia compared to a healthy pregnancy (P = 0.034; 0.302(0.217–0.421) vs. 0.171(0.110–0.266)). MiR210-3p showed no significant difference between the two groups (P = 0.951). The adjustment of miR518b was made for a gestational age and smoking status and the difference between the preeclampsia and healthy pregnancy group was more significant (P = 0.026; 0.300(0.216–0.419) vs. 0.172(0.121–0.245)). Plasma miR-518b was significantly higher in the group of preeclampsia patients with proteinuria above the 75th percentile for the group (P=0.033), in women who smoked (P=0.039), and was positively related to uric acid in preeclampsia (P = 0.018, r = 0.536). Plasma miR518b was able to significantly discriminate between preeclampsia and healthy pregnancy, yielding AUC of 0.712 (95%CI:0.539–0.891), P = 0.028. Conclusions: In this study plasma microRNA were measured for the first time in preeclampsia and healthy pregnancies with ddPCR. Placenta-specific miR-518b could serve as a potential biomarker for discriminating preeclampsia and healthy pregnancy, which should be confirmed on a larger study population. This study has failed to confirm the same potential for miR210-3p.",
publisher = "Elsevier",
journal = "Clinical Biochemistry",
title = "Placenta-specific plasma miR518b is a potential biomarker for preeclampsia",
volume = "79",
pages = "28-33",
doi = "10.1016/j.clinbiochem.2020.02.012"
}

Munjas, J., Sopić, M., Joksić, I., Prosenc Zmrzljak, U., Karadžov-Orlić, N., Košir, R., Egić, A., Miković, Ž., Ninić, A.,& Spasojević-Kalimanovska, V.. (2020). Placenta-specific plasma miR518b is a potential biomarker for preeclampsia. in Clinical Biochemistry
Elsevier., 79, 28-33.
https://doi.org/10.1016/j.clinbiochem.2020.02.012

Munjas J, Sopić M, Joksić I, Prosenc Zmrzljak U, Karadžov-Orlić N, Košir R, Egić A, Miković Ž, Ninić A, Spasojević-Kalimanovska V. Placenta-specific plasma miR518b is a potential biomarker for preeclampsia. in Clinical Biochemistry. 2020;79:28-33.
doi:10.1016/j.clinbiochem.2020.02.012 .

Munjas, Jelena, Sopić, Miron, Joksić, Ivana, Prosenc Zmrzljak, Ursula, Karadžov-Orlić, Nataša, Košir, Rok, Egić, Amira, Miković, Željko, Ninić, Ana, Spasojević-Kalimanovska, Vesna, "Placenta-specific plasma miR518b is a potential biomarker for preeclampsia" in Clinical Biochemistry, 79 (2020):28-33,
https://doi.org/10.1016/j.clinbiochem.2020.02.012 . .

TY  - JOUR
AU  - Janać, Jelena
AU  - Zeljković, Aleksandra
AU  - Jelić-Ivanović, Zorana
AU  - Dimitrijević-Srećković, Vesna
AU  - Vekić, Jelena
AU  - Miljković, Milica
AU  - Stefanović, Aleksandra
AU  - Kotur-Stevuljević, Jelena
AU  - Ivanišević, Jasmina
AU  - Spasojević-Kalimanovska, Vesna
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3803
AB  - Background: We evaluated the qualitative characteristics of high-density lipoprotein (HDL) particles in metabolically healthy and unhealthy overweight and obese subjects. Methods: The study involved 115 subject individuals classified as metabolically healthy and unhealthy, as in overweight and obese groups. Commercial enzyme-linked immunosorbent assay (ELISA) kits were used to measure oxidized HDL (OxHDL) and serum amyloid A (SAA) concentrations. Lipoprotein subfractions were separated using nondenaturing gradient gel electrophoresis. Results: An independent association was shown between increased OxHDL/HDL-cholesterol ratio and the occurrence of metabolically unhealthy phenotype in the overweight and obese groups. The OxHDL/HDL-cholesterol ratio showed excellent and acceptable diagnostic accuracy in determination of metabolic health phenotypes (overweight group, AUC = 0.881; obese group, AUC = 0.765). Accumulation of smaller HDL particles in metabolically unhealthy subjects was verified by lipoprotein subfraction analysis. SAA concentrations did not differ significantly between phenotypes. Conclusions: Increased OxHDL/HDL-cholesterol ratio may be a potential indicator of disturbed metabolic health in overweight and obese individuals.
PB  - Oxford University Press
T2  - Lab Medicine
T1  - Increased oxidized high-density lipoprotein/ high-density lipoprotein-cholesterol ratio as a potential indicator of disturbed metabolic health in overweight and obese individuals
VL  - 51
IS  - 1
SP  - 24
EP  - 33
DO  - 10.1093/labmed/lmz017
ER  - 

@article{
author = "Janać, Jelena and Zeljković, Aleksandra and Jelić-Ivanović, Zorana and Dimitrijević-Srećković, Vesna and Vekić, Jelena and Miljković, Milica and Stefanović, Aleksandra and Kotur-Stevuljević, Jelena and Ivanišević, Jasmina and Spasojević-Kalimanovska, Vesna",
year = "2020",
abstract = "Background: We evaluated the qualitative characteristics of high-density lipoprotein (HDL) particles in metabolically healthy and unhealthy overweight and obese subjects. Methods: The study involved 115 subject individuals classified as metabolically healthy and unhealthy, as in overweight and obese groups. Commercial enzyme-linked immunosorbent assay (ELISA) kits were used to measure oxidized HDL (OxHDL) and serum amyloid A (SAA) concentrations. Lipoprotein subfractions were separated using nondenaturing gradient gel electrophoresis. Results: An independent association was shown between increased OxHDL/HDL-cholesterol ratio and the occurrence of metabolically unhealthy phenotype in the overweight and obese groups. The OxHDL/HDL-cholesterol ratio showed excellent and acceptable diagnostic accuracy in determination of metabolic health phenotypes (overweight group, AUC = 0.881; obese group, AUC = 0.765). Accumulation of smaller HDL particles in metabolically unhealthy subjects was verified by lipoprotein subfraction analysis. SAA concentrations did not differ significantly between phenotypes. Conclusions: Increased OxHDL/HDL-cholesterol ratio may be a potential indicator of disturbed metabolic health in overweight and obese individuals.",
publisher = "Oxford University Press",
journal = "Lab Medicine",
title = "Increased oxidized high-density lipoprotein/ high-density lipoprotein-cholesterol ratio as a potential indicator of disturbed metabolic health in overweight and obese individuals",
volume = "51",
number = "1",
pages = "24-33",
doi = "10.1093/labmed/lmz017"
}

Janać, J., Zeljković, A., Jelić-Ivanović, Z., Dimitrijević-Srećković, V., Vekić, J., Miljković, M., Stefanović, A., Kotur-Stevuljević, J., Ivanišević, J.,& Spasojević-Kalimanovska, V.. (2020). Increased oxidized high-density lipoprotein/ high-density lipoprotein-cholesterol ratio as a potential indicator of disturbed metabolic health in overweight and obese individuals. in Lab Medicine
Oxford University Press., 51(1), 24-33.
https://doi.org/10.1093/labmed/lmz017

Janać J, Zeljković A, Jelić-Ivanović Z, Dimitrijević-Srećković V, Vekić J, Miljković M, Stefanović A, Kotur-Stevuljević J, Ivanišević J, Spasojević-Kalimanovska V. Increased oxidized high-density lipoprotein/ high-density lipoprotein-cholesterol ratio as a potential indicator of disturbed metabolic health in overweight and obese individuals. in Lab Medicine. 2020;51(1):24-33.
doi:10.1093/labmed/lmz017 .

Janać, Jelena, Zeljković, Aleksandra, Jelić-Ivanović, Zorana, Dimitrijević-Srećković, Vesna, Vekić, Jelena, Miljković, Milica, Stefanović, Aleksandra, Kotur-Stevuljević, Jelena, Ivanišević, Jasmina, Spasojević-Kalimanovska, Vesna, "Increased oxidized high-density lipoprotein/ high-density lipoprotein-cholesterol ratio as a potential indicator of disturbed metabolic health in overweight and obese individuals" in Lab Medicine, 51, no. 1 (2020):24-33,
https://doi.org/10.1093/labmed/lmz017 . .

TY  - JOUR
AU  - Vekić, Jelena
AU  - Ivanišević, Jasmina
AU  - Zeljković, Aleksandra
AU  - Spasojević-Kalimanovska, Vesna
AU  - Bogavac-Stanojević, Nataša
AU  - Mihajlović, Marija
AU  - Janać, Jelena
AU  - Vujčić, Sanja
AU  - Miljković, Milica
AU  - Zujović, Dejan
AU  - Kotur-Stevuljević, Jelena
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3746
AB  - Background:Propolis  and  N-acetylcysteine  have  positiveimpact  on  respiratory  tract  health.  Also,  it  has  been  sug-gested that they have beneficial effects on serum lipid andoxidative  stress  status,  but  the  available  data  are  limitedand  mostly  gained  from  animal  models.  In  this  study  weevaluated the effects of propolis and N-acetylcysteine sup-plementation  (PropoMucil®)  on  lipid  status,  lipoproteinsubclasses  distribution  and  paraoxonase  1  activity  in  sub-jects with acute respiratory infection.Methods:Twenty  subjects  with  acute  respiratory  infectionwere included. PropoMucil®granules for oral solution (80mg of dry propolis extract and 200 mg of N-acetylcysteine)were administered tree times per day for ten days. Serumlipid  profile,  paraoxonase  1  activity  and  low-density  andhigh-density  lipoprotein  size  and  subclasses  distributionwere assessed at baseline and after supplementation.Results:Following ten days of supplementation lipid statusremained  unchanged,  but  a  significant  increase  of  low-density lipoprotein particle size and proportion of high-den-sity   lipoprotein   3a   particles   were   found   (P<0.05).Moreover, supplementation with PropoMucil®significantlyimproved  high-density  lipoprotein  particles  distribution, particularly  in  those  who  smoke.  There  was  a  moderateincrease  of  paraoxonase  1  activity,  but  without  statisticalsignificance.Conclusions:The presented study demonstrated that short-term  supplementation  with  PropoMucil®has  beneficialeffects  on  low-density  and  high-density  lipoprotein  sub-classes  distribution  and  paraoxonase  1  activity  in  subjectswith  acute  respiratory  infection  particularly  in  those  whosmoke.
AB  - Uvod: Propolis i N-acetilcistein pozitivno utiču na zdravlje disajnih puteva. Takođe, sugeriše se da oni imaju blagotvorno dejstvo na lipidni i oksidativno-stresni status, ali podaci su ograničeni i dobijeni uglavnom na životinjskim modelima. U ovom istraživanju, procenili smo kombinovani efekat propolisa i N-acetilcisteina (PropoMucil®) na status lipida, raspodelu supklasa lipoproteina i aktivnost paraoksonaze 1 kod pacijenata sa akutnom respiratornom infekcijom (ARI). Metode: Uključeno je 20 ispitanika sa akutnom respiratornom infekcijom. Oralni rastvor praška PropoMucil® (80 mg suvog ekstrakta propolisa i 200 mg N-acetilcisteina) je aplikovan 3 puta dnevno tokom 10 dana. Serumski lipidni profil, aktivnost praoksonaze 1 i veličina i raspodela supfrakcija lipoproteina niske gustine i lipoproteina visoke gustine su određeni pre i nakon suplementacije. Rezultati: Nakon deset dana suplementacije, lipidni status je ostao nepromenjen, ali je utvrđeno značajno povećanje veličine lipoproteina niske gustine i relativnog udela lipoproteina visoke gustine 3a (P <0,05). Pored toga, suplementacija PropoMucil®-om značajno je poboljšala distribuciju lipoproteinskih čestica visoke gustine, posebno kod pacijenata koji puše. Došlo je do umerenog porasta aktivnosti paraoksonaze 1, ali bez statističke značajnosti. Zaključak: Studija je pokazala da kratkotrajna suplementacija PropoMucil®-om ima korisne efekte na distribuciju supklasa lipoproteina niske i visoke gustine i aktivnost paraoksonaze 1 kod ispitanika sa akutnom respiratornom infekcijom, posebno kod onih koji puše.
PB  - Beograd : Društvo medicinskih biohemičara Srbije
T2  - Journal of Medical Biochemistry
T1  - Effect of propolis and N-acetylcysteine supplementation on lipoprotein subclasses distribution and paraoxonase 1 activity in subjects with acute respiratory infection
T1  - Efekat suplementacije propolisom i N-acetilcisteinom na raspodelu lipoproteinskih supklasa i aktivnosti paraoksonaze 1 u osoba sa akutnom respiratornom infekcijom
VL  - 39
IS  - 4
SP  - 467
EP  - 473
DO  - 10.5937/jomb0-24695
ER  - 

@article{
author = "Vekić, Jelena and Ivanišević, Jasmina and Zeljković, Aleksandra and Spasojević-Kalimanovska, Vesna and Bogavac-Stanojević, Nataša and Mihajlović, Marija and Janać, Jelena and Vujčić, Sanja and Miljković, Milica and Zujović, Dejan and Kotur-Stevuljević, Jelena",
year = "2020",
abstract = "Background:Propolis  and  N-acetylcysteine  have  positiveimpact  on  respiratory  tract  health.  Also,  it  has  been  sug-gested that they have beneficial effects on serum lipid andoxidative  stress  status,  but  the  available  data  are  limitedand  mostly  gained  from  animal  models.  In  this  study  weevaluated the effects of propolis and N-acetylcysteine sup-plementation  (PropoMucil®)  on  lipid  status,  lipoproteinsubclasses  distribution  and  paraoxonase  1  activity  in  sub-jects with acute respiratory infection.Methods:Twenty  subjects  with  acute  respiratory  infectionwere included. PropoMucil®granules for oral solution (80mg of dry propolis extract and 200 mg of N-acetylcysteine)were administered tree times per day for ten days. Serumlipid  profile,  paraoxonase  1  activity  and  low-density  andhigh-density  lipoprotein  size  and  subclasses  distributionwere assessed at baseline and after supplementation.Results:Following ten days of supplementation lipid statusremained  unchanged,  but  a  significant  increase  of  low-density lipoprotein particle size and proportion of high-den-sity   lipoprotein   3a   particles   were   found   (P<0.05).Moreover, supplementation with PropoMucil®significantlyimproved  high-density  lipoprotein  particles  distribution, particularly  in  those  who  smoke.  There  was  a  moderateincrease  of  paraoxonase  1  activity,  but  without  statisticalsignificance.Conclusions:The presented study demonstrated that short-term  supplementation  with  PropoMucil®has  beneficialeffects  on  low-density  and  high-density  lipoprotein  sub-classes  distribution  and  paraoxonase  1  activity  in  subjectswith  acute  respiratory  infection  particularly  in  those  whosmoke., Uvod: Propolis i N-acetilcistein pozitivno utiču na zdravlje disajnih puteva. Takođe, sugeriše se da oni imaju blagotvorno dejstvo na lipidni i oksidativno-stresni status, ali podaci su ograničeni i dobijeni uglavnom na životinjskim modelima. U ovom istraživanju, procenili smo kombinovani efekat propolisa i N-acetilcisteina (PropoMucil®) na status lipida, raspodelu supklasa lipoproteina i aktivnost paraoksonaze 1 kod pacijenata sa akutnom respiratornom infekcijom (ARI). Metode: Uključeno je 20 ispitanika sa akutnom respiratornom infekcijom. Oralni rastvor praška PropoMucil® (80 mg suvog ekstrakta propolisa i 200 mg N-acetilcisteina) je aplikovan 3 puta dnevno tokom 10 dana. Serumski lipidni profil, aktivnost praoksonaze 1 i veličina i raspodela supfrakcija lipoproteina niske gustine i lipoproteina visoke gustine su određeni pre i nakon suplementacije. Rezultati: Nakon deset dana suplementacije, lipidni status je ostao nepromenjen, ali je utvrđeno značajno povećanje veličine lipoproteina niske gustine i relativnog udela lipoproteina visoke gustine 3a (P <0,05). Pored toga, suplementacija PropoMucil®-om značajno je poboljšala distribuciju lipoproteinskih čestica visoke gustine, posebno kod pacijenata koji puše. Došlo je do umerenog porasta aktivnosti paraoksonaze 1, ali bez statističke značajnosti. Zaključak: Studija je pokazala da kratkotrajna suplementacija PropoMucil®-om ima korisne efekte na distribuciju supklasa lipoproteina niske i visoke gustine i aktivnost paraoksonaze 1 kod ispitanika sa akutnom respiratornom infekcijom, posebno kod onih koji puše.",
publisher = "Beograd : Društvo medicinskih biohemičara Srbije",
journal = "Journal of Medical Biochemistry",
title = "Effect of propolis and N-acetylcysteine supplementation on lipoprotein subclasses distribution and paraoxonase 1 activity in subjects with acute respiratory infection, Efekat suplementacije propolisom i N-acetilcisteinom na raspodelu lipoproteinskih supklasa i aktivnosti paraoksonaze 1 u osoba sa akutnom respiratornom infekcijom",
volume = "39",
number = "4",
pages = "467-473",
doi = "10.5937/jomb0-24695"
}

Vekić, J., Ivanišević, J., Zeljković, A., Spasojević-Kalimanovska, V., Bogavac-Stanojević, N., Mihajlović, M., Janać, J., Vujčić, S., Miljković, M., Zujović, D.,& Kotur-Stevuljević, J.. (2020). Effect of propolis and N-acetylcysteine supplementation on lipoprotein subclasses distribution and paraoxonase 1 activity in subjects with acute respiratory infection. in Journal of Medical Biochemistry
Beograd : Društvo medicinskih biohemičara Srbije., 39(4), 467-473.
https://doi.org/10.5937/jomb0-24695

Vekić J, Ivanišević J, Zeljković A, Spasojević-Kalimanovska V, Bogavac-Stanojević N, Mihajlović M, Janać J, Vujčić S, Miljković M, Zujović D, Kotur-Stevuljević J. Effect of propolis and N-acetylcysteine supplementation on lipoprotein subclasses distribution and paraoxonase 1 activity in subjects with acute respiratory infection. in Journal of Medical Biochemistry. 2020;39(4):467-473.
doi:10.5937/jomb0-24695 .

Vekić, Jelena, Ivanišević, Jasmina, Zeljković, Aleksandra, Spasojević-Kalimanovska, Vesna, Bogavac-Stanojević, Nataša, Mihajlović, Marija, Janać, Jelena, Vujčić, Sanja, Miljković, Milica, Zujović, Dejan, Kotur-Stevuljević, Jelena, "Effect of propolis and N-acetylcysteine supplementation on lipoprotein subclasses distribution and paraoxonase 1 activity in subjects with acute respiratory infection" in Journal of Medical Biochemistry, 39, no. 4 (2020):467-473,
https://doi.org/10.5937/jomb0-24695 . .

TY  - JOUR
AU  - Zeljković, Aleksandra
AU  - Mihajlović, Marija
AU  - Stefanović, Aleksandra
AU  - Zeljković, D.
AU  - Trifunović, B.
AU  - Miljković, Milica
AU  - Spasojević-Kalimanovska, Vesna
AU  - Vekić, Jelena
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3739
AB  - Aim: Despite many efforts, reliable biomarkers for the prediction and diagnosis of colorectal cancer (CRC) are still missing. Insulin-like growth factor 1 (IGF-1) and E-cadherin are recognized as potential biomarkers, but their diagnostic capacity is largely unexplored in CRC. The aim of this work is to investigate IGF-1 and E-cadherin levels with respect to various characteristics of CRC and to estimate their diagnostic potential. Method: Seventy CRC patients and 75 healthy individuals were enrolled. IGF-1 and E-cadherin were determined using enzyme-linked immunosorbent assay. The predictive and diagnostic capacities of IGF-1 and E-cadherin were estimated by logistic regression analysis and by determination of the area under the receiver operating characteristic (ROC) curve (AUC). Results: Concentrations of IGF-1 were lower (P = 0.019) while levels of E-cadherin were higher (P < 0.001) in CRC patients than in controls. IGF-1 concentration decreased in parallel with age and progression of CRC (P = 0.023). Also, IGF-1 was higher in men with CRC than in women (P = 0.003). E-cadherin levels were unaffected by variations in either anthropometric characteristics of CRC patients, or localization, grade and stage of the tumour. Both IGF-1 and E-cadherin were independently associated with CRC (P = 0.040; P < 0.001, respectively). The diagnostic accuracy of IGF-1 was estimated as acceptable (AUC = 0.757; P < 0.001), while the diagnostic accuracy of E-cadherin was outstanding (AUC = 0.954; P < 0.001). Conclusions: Decreased IGF-1 and increased E-cadherin levels were found in CRC patients. IGF-1, but not E-cadherin, concentrations differed according to age, gender and stage of CRC. Both markers were independently associated with the presence of the disease, while E-cadherin demonstrated high diagnostic accuracy.
PB  - Blackwell Publishing Ltd
T2  - Colorectal Disease
T1  - Potential use of serum insulin-like growth factor 1 and E-cadherin as biomarkers of colorectal cancer
DO  - 10.1111/codi.15360
ER  - 

@article{
author = "Zeljković, Aleksandra and Mihajlović, Marija and Stefanović, Aleksandra and Zeljković, D. and Trifunović, B. and Miljković, Milica and Spasojević-Kalimanovska, Vesna and Vekić, Jelena",
year = "2020",
abstract = "Aim: Despite many efforts, reliable biomarkers for the prediction and diagnosis of colorectal cancer (CRC) are still missing. Insulin-like growth factor 1 (IGF-1) and E-cadherin are recognized as potential biomarkers, but their diagnostic capacity is largely unexplored in CRC. The aim of this work is to investigate IGF-1 and E-cadherin levels with respect to various characteristics of CRC and to estimate their diagnostic potential. Method: Seventy CRC patients and 75 healthy individuals were enrolled. IGF-1 and E-cadherin were determined using enzyme-linked immunosorbent assay. The predictive and diagnostic capacities of IGF-1 and E-cadherin were estimated by logistic regression analysis and by determination of the area under the receiver operating characteristic (ROC) curve (AUC). Results: Concentrations of IGF-1 were lower (P = 0.019) while levels of E-cadherin were higher (P < 0.001) in CRC patients than in controls. IGF-1 concentration decreased in parallel with age and progression of CRC (P = 0.023). Also, IGF-1 was higher in men with CRC than in women (P = 0.003). E-cadherin levels were unaffected by variations in either anthropometric characteristics of CRC patients, or localization, grade and stage of the tumour. Both IGF-1 and E-cadherin were independently associated with CRC (P = 0.040; P < 0.001, respectively). The diagnostic accuracy of IGF-1 was estimated as acceptable (AUC = 0.757; P < 0.001), while the diagnostic accuracy of E-cadherin was outstanding (AUC = 0.954; P < 0.001). Conclusions: Decreased IGF-1 and increased E-cadherin levels were found in CRC patients. IGF-1, but not E-cadherin, concentrations differed according to age, gender and stage of CRC. Both markers were independently associated with the presence of the disease, while E-cadherin demonstrated high diagnostic accuracy.",
publisher = "Blackwell Publishing Ltd",
journal = "Colorectal Disease",
title = "Potential use of serum insulin-like growth factor 1 and E-cadherin as biomarkers of colorectal cancer",
doi = "10.1111/codi.15360"
}

Zeljković, A., Mihajlović, M., Stefanović, A., Zeljković, D., Trifunović, B., Miljković, M., Spasojević-Kalimanovska, V.,& Vekić, J.. (2020). Potential use of serum insulin-like growth factor 1 and E-cadherin as biomarkers of colorectal cancer. in Colorectal Disease
Blackwell Publishing Ltd..
https://doi.org/10.1111/codi.15360

Zeljković A, Mihajlović M, Stefanović A, Zeljković D, Trifunović B, Miljković M, Spasojević-Kalimanovska V, Vekić J. Potential use of serum insulin-like growth factor 1 and E-cadherin as biomarkers of colorectal cancer. in Colorectal Disease. 2020;.
doi:10.1111/codi.15360 .

Zeljković, Aleksandra, Mihajlović, Marija, Stefanović, Aleksandra, Zeljković, D., Trifunović, B., Miljković, Milica, Spasojević-Kalimanovska, Vesna, Vekić, Jelena, "Potential use of serum insulin-like growth factor 1 and E-cadherin as biomarkers of colorectal cancer" in Colorectal Disease (2020),
https://doi.org/10.1111/codi.15360 . .

TY  - JOUR
AU  - Kotur-Stevuljević, Jelena
AU  - Vekić, Jelena
AU  - Stefanović, Aleksandra
AU  - Zeljković, Aleksandra
AU  - Ninić, Ana
AU  - Ivanišević, Jasmina
AU  - Miljković, Milica
AU  - Sopić, Miron
AU  - Munjas, Jelena
AU  - Mihajlović, Marija
AU  - Spasić, Slavica
AU  - Jelić-Ivanović, Zorana
AU  - Spasojević-Kalimanovska, Vesna
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3615
AB  - A direct and an indirect relationship between paraoxonase 1 (PON1) and atherosclerosis exists. Given PON1's physical location within high-density lipoprotein (HDL) particles and its recognized enzyme activity, it is certainly reasonable to suggest that PON1 facilitates the antiatherogenic nature of HDL particles. PON1 also plays a role in regulating reverse cholesterol transport, antioxidative, anti-inflammatory, antiapoptotic, vasodilative, and antithrombotic activities and several endothelial cell functions. HDL dysfunctionality is a more recent issue and seems to be centered on pathological conditions affecting HDL structure and size profiles. This review is focused on the role of PON1 status in different atherosclerosis-related diseases that we have studied over the last twenty years (coronary heart disease, acute ischemic stroke, diabetes mellitus type 2, end-stage renal disease, chronic obstructive pulmonary disease, and sarcoidosis) with the aim to determine the true value of PON1 as a biomarker. The role of PON1 in cancer is also covered, as risk factors and mechanisms underlying both atherosclerosis and cancer share common features. © 2019 International Union of Biochemistry and Molecular Biology
PB  - Blackwell Publishing Inc.
T2  - BioFactors
T1  - Paraoxonase 1 and atherosclerosis-related diseases
VL  - 46
IS  - 2
SP  - 193
EP  - 205
DO  - 10.1002/biof.1549
ER  - 

@article{
author = "Kotur-Stevuljević, Jelena and Vekić, Jelena and Stefanović, Aleksandra and Zeljković, Aleksandra and Ninić, Ana and Ivanišević, Jasmina and Miljković, Milica and Sopić, Miron and Munjas, Jelena and Mihajlović, Marija and Spasić, Slavica and Jelić-Ivanović, Zorana and Spasojević-Kalimanovska, Vesna",
year = "2020",
abstract = "A direct and an indirect relationship between paraoxonase 1 (PON1) and atherosclerosis exists. Given PON1's physical location within high-density lipoprotein (HDL) particles and its recognized enzyme activity, it is certainly reasonable to suggest that PON1 facilitates the antiatherogenic nature of HDL particles. PON1 also plays a role in regulating reverse cholesterol transport, antioxidative, anti-inflammatory, antiapoptotic, vasodilative, and antithrombotic activities and several endothelial cell functions. HDL dysfunctionality is a more recent issue and seems to be centered on pathological conditions affecting HDL structure and size profiles. This review is focused on the role of PON1 status in different atherosclerosis-related diseases that we have studied over the last twenty years (coronary heart disease, acute ischemic stroke, diabetes mellitus type 2, end-stage renal disease, chronic obstructive pulmonary disease, and sarcoidosis) with the aim to determine the true value of PON1 as a biomarker. The role of PON1 in cancer is also covered, as risk factors and mechanisms underlying both atherosclerosis and cancer share common features. © 2019 International Union of Biochemistry and Molecular Biology",
publisher = "Blackwell Publishing Inc.",
journal = "BioFactors",
title = "Paraoxonase 1 and atherosclerosis-related diseases",
volume = "46",
number = "2",
pages = "193-205",
doi = "10.1002/biof.1549"
}

Kotur-Stevuljević, J., Vekić, J., Stefanović, A., Zeljković, A., Ninić, A., Ivanišević, J., Miljković, M., Sopić, M., Munjas, J., Mihajlović, M., Spasić, S., Jelić-Ivanović, Z.,& Spasojević-Kalimanovska, V.. (2020). Paraoxonase 1 and atherosclerosis-related diseases. in BioFactors
Blackwell Publishing Inc.., 46(2), 193-205.
https://doi.org/10.1002/biof.1549

Kotur-Stevuljević J, Vekić J, Stefanović A, Zeljković A, Ninić A, Ivanišević J, Miljković M, Sopić M, Munjas J, Mihajlović M, Spasić S, Jelić-Ivanović Z, Spasojević-Kalimanovska V. Paraoxonase 1 and atherosclerosis-related diseases. in BioFactors. 2020;46(2):193-205.
doi:10.1002/biof.1549 .

Kotur-Stevuljević, Jelena, Vekić, Jelena, Stefanović, Aleksandra, Zeljković, Aleksandra, Ninić, Ana, Ivanišević, Jasmina, Miljković, Milica, Sopić, Miron, Munjas, Jelena, Mihajlović, Marija, Spasić, Slavica, Jelić-Ivanović, Zorana, Spasojević-Kalimanovska, Vesna, "Paraoxonase 1 and atherosclerosis-related diseases" in BioFactors, 46, no. 2 (2020):193-205,
https://doi.org/10.1002/biof.1549 . .

TY  - JOUR
AU  - Vladimirov, Sandra
AU  - Gojković, Tamara
AU  - Zeljković, Aleksandra
AU  - Jelić-Ivanović, Zorana
AU  - Spasojević-Kalimanovska, Vesna
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3653
AB  - Background:Non-cholesterol  sterols  (NCS)  are  promising biomarkers for estimation of cholesterol homeostasis properties.  In  addition,  determination  of  NCS  in  high-density lipoprotein (HDL) fraction (HDL-NCS) could provide information on cholesterol efflux. However, matrix effects interfere in liquid chromatography–mass spectrometry (LC-MS)analysis  of  NCS,  thereby  impairing  the  method  sensitivity.The aims of this study were development, optimization and validation  of  LC-MS  method  for  quantification  of  NCS  in serum  and  HDL-NCS.  Additionally,  matrix  effect  interfer-ences  and  methods  application  in  individual  serum  samples were examined.Methods:HDL precipitating reagent was used for HDL isolation. Matrix effect was examined by comparing different surrogates  by  simple  regression  analysis.  Validation  was conducted according to the FDA-ICH guideline. 20 healthy volunteers were recruited for testing of method application.Results:The  observed  matrix  effect  was  30%,  and  matrix comparison  showed  that  cholesterol  was  the  dominant contributor  to  the  matrix  effect.  Cholesterol  concentration was  adjusted  by  construction  of  the  calibration  curve  for serum  and  HDL  fraction  (5  mmol/L  and  2.5  mmol/L,respectively). The intra- and inter- run variabilities for NCSs were 4.7–10.3% for serum NCS and 3.6–13.6% for HDL-NCS  and  4.6–9.5%  for  serum  NCSs  and  2.5–9.8%  for HDL-NCS,  respectively.  Recovery  studies  showed  satisfactory  results  for  NCSs:  89.8–113.1%  for  serum  NCS  and85.3–95.8% for HDL-NCS. Conclusions:The  method  was  successfully  developed  and optimized. The matrix interference was solved by customising  calibration  curves  for  each  method  and  sample  type.The  measurement  of  NCS  in  HDL  fraction  was  proposed for the first time as potentially useful procedure in biomedical researches.
AB  - Uvod: Neholesterolski steroli (NHS) predstavljaju potencijalne biomarkere homeostaze holesterola. Pored toga, određivanje NHS u HDL frakciji (HDL-NHS) moglo bi da pruži dodatne informacije o efluksu holesterola. Međutim, određivanje NHS metodom tečne hromatografije - masene spektrometrije (LC-MS) je podložno uticaju matriksa usled čega dolazi do sniženja osetljivost metode. Studija je prvenstveno imala za cilj razvoj, optimizaciju i validaciju LC-MS metode za kvantifikaciju NHS u serumu i HDL-NHS. Pored toga, detaljno je ispitan efekat matriksa i metoda primenjena za analizu uzoraka seruma zdravih dobrovoljaca. Metode: Izolacija HDL frakcije je izvršena upotrebom precipitirajućeg reagensa. Efekat matriksa je ispitan poređenjem različitih surogat matriksa regresionom analizom. Validacija je sprovedena u skladu sa FDA-ICH vodičem za validaciju bioanalitičkih metoda. Korišćeni su serumi 20 zdravih dobrovoljaca u cilju kliničke evaluacije metode. Rezultati: Efekat matriksa je iznosio 30% i dalja analiza je pokazala da je holesterol bio glavni uzročnik efekta matriksa. U cilju rešavanja ove interferencije, kalibracione krive za su pripremljene u metanolnom rastvoru holesterola koncentracije 5 mmol/L, a za kvantifikaciju HDL-NHS u metanolnom rastvoru holesterola koncentracije 2,5 mmol/L. Varijacije unutar serije su bile 4,7-10,3% za NHS i 3,6-13,6% za HDL-NHS, a između serija 4,6-9,5% za NHS i 2,5-9,8% za HDL-NHS. Studije prinosa analita su pokazale zadovoljavajuće rezultate: 89,8-113,1% za NHS i 85,3-95,8% za HDL-NHS. Zaključak: Metoda je uspešno optimizovana i validirana. Interferencija usled prisustva holesterola je korigovana upotrebom odgovarajućih kalibracionih krivih za svaku vrstu uzorka. Određivanje HDL-NHS je po prvi put predloženo kao potencijalno korisna procedura u biomedicinskim istraživanjima.
PB  - Belgrade : Society of Medical Biochemists of Serbia
T2  - Journal of Medical Biochemistry
T1  - Determination of non-cholesterol sterols in serum and HDL fraction by LC/MS-ms: Significance of matrix-related interferences
T1  - Određivanje neholesterolskih sterola u serumu i HDL frakciji upotrebom LC/MS-MS - ispitivanje uticaja matriksa
VL  - 39
SP  - 299
EP  - 308
DO  - 10.2478/jomb-2019-0044
ER  - 

@article{
author = "Vladimirov, Sandra and Gojković, Tamara and Zeljković, Aleksandra and Jelić-Ivanović, Zorana and Spasojević-Kalimanovska, Vesna",
year = "2020",
abstract = "Background:Non-cholesterol  sterols  (NCS)  are  promising biomarkers for estimation of cholesterol homeostasis properties.  In  addition,  determination  of  NCS  in  high-density lipoprotein (HDL) fraction (HDL-NCS) could provide information on cholesterol efflux. However, matrix effects interfere in liquid chromatography–mass spectrometry (LC-MS)analysis  of  NCS,  thereby  impairing  the  method  sensitivity.The aims of this study were development, optimization and validation  of  LC-MS  method  for  quantification  of  NCS  in serum  and  HDL-NCS.  Additionally,  matrix  effect  interfer-ences  and  methods  application  in  individual  serum  samples were examined.Methods:HDL precipitating reagent was used for HDL isolation. Matrix effect was examined by comparing different surrogates  by  simple  regression  analysis.  Validation  was conducted according to the FDA-ICH guideline. 20 healthy volunteers were recruited for testing of method application.Results:The  observed  matrix  effect  was  30%,  and  matrix comparison  showed  that  cholesterol  was  the  dominant contributor  to  the  matrix  effect.  Cholesterol  concentration was  adjusted  by  construction  of  the  calibration  curve  for serum  and  HDL  fraction  (5  mmol/L  and  2.5  mmol/L,respectively). The intra- and inter- run variabilities for NCSs were 4.7–10.3% for serum NCS and 3.6–13.6% for HDL-NCS  and  4.6–9.5%  for  serum  NCSs  and  2.5–9.8%  for HDL-NCS,  respectively.  Recovery  studies  showed  satisfactory  results  for  NCSs:  89.8–113.1%  for  serum  NCS  and85.3–95.8% for HDL-NCS. Conclusions:The  method  was  successfully  developed  and optimized. The matrix interference was solved by customising  calibration  curves  for  each  method  and  sample  type.The  measurement  of  NCS  in  HDL  fraction  was  proposed for the first time as potentially useful procedure in biomedical researches., Uvod: Neholesterolski steroli (NHS) predstavljaju potencijalne biomarkere homeostaze holesterola. Pored toga, određivanje NHS u HDL frakciji (HDL-NHS) moglo bi da pruži dodatne informacije o efluksu holesterola. Međutim, određivanje NHS metodom tečne hromatografije - masene spektrometrije (LC-MS) je podložno uticaju matriksa usled čega dolazi do sniženja osetljivost metode. Studija je prvenstveno imala za cilj razvoj, optimizaciju i validaciju LC-MS metode za kvantifikaciju NHS u serumu i HDL-NHS. Pored toga, detaljno je ispitan efekat matriksa i metoda primenjena za analizu uzoraka seruma zdravih dobrovoljaca. Metode: Izolacija HDL frakcije je izvršena upotrebom precipitirajućeg reagensa. Efekat matriksa je ispitan poređenjem različitih surogat matriksa regresionom analizom. Validacija je sprovedena u skladu sa FDA-ICH vodičem za validaciju bioanalitičkih metoda. Korišćeni su serumi 20 zdravih dobrovoljaca u cilju kliničke evaluacije metode. Rezultati: Efekat matriksa je iznosio 30% i dalja analiza je pokazala da je holesterol bio glavni uzročnik efekta matriksa. U cilju rešavanja ove interferencije, kalibracione krive za su pripremljene u metanolnom rastvoru holesterola koncentracije 5 mmol/L, a za kvantifikaciju HDL-NHS u metanolnom rastvoru holesterola koncentracije 2,5 mmol/L. Varijacije unutar serije su bile 4,7-10,3% za NHS i 3,6-13,6% za HDL-NHS, a između serija 4,6-9,5% za NHS i 2,5-9,8% za HDL-NHS. Studije prinosa analita su pokazale zadovoljavajuće rezultate: 89,8-113,1% za NHS i 85,3-95,8% za HDL-NHS. Zaključak: Metoda je uspešno optimizovana i validirana. Interferencija usled prisustva holesterola je korigovana upotrebom odgovarajućih kalibracionih krivih za svaku vrstu uzorka. Određivanje HDL-NHS je po prvi put predloženo kao potencijalno korisna procedura u biomedicinskim istraživanjima.",
publisher = "Belgrade : Society of Medical Biochemists of Serbia",
journal = "Journal of Medical Biochemistry",
title = "Determination of non-cholesterol sterols in serum and HDL fraction by LC/MS-ms: Significance of matrix-related interferences, Određivanje neholesterolskih sterola u serumu i HDL frakciji upotrebom LC/MS-MS - ispitivanje uticaja matriksa",
volume = "39",
pages = "299-308",
doi = "10.2478/jomb-2019-0044"
}

Vladimirov, S., Gojković, T., Zeljković, A., Jelić-Ivanović, Z.,& Spasojević-Kalimanovska, V.. (2020). Determination of non-cholesterol sterols in serum and HDL fraction by LC/MS-ms: Significance of matrix-related interferences. in Journal of Medical Biochemistry
Belgrade : Society of Medical Biochemists of Serbia., 39, 299-308.
https://doi.org/10.2478/jomb-2019-0044

Vladimirov S, Gojković T, Zeljković A, Jelić-Ivanović Z, Spasojević-Kalimanovska V. Determination of non-cholesterol sterols in serum and HDL fraction by LC/MS-ms: Significance of matrix-related interferences. in Journal of Medical Biochemistry. 2020;39:299-308.
doi:10.2478/jomb-2019-0044 .

Vladimirov, Sandra, Gojković, Tamara, Zeljković, Aleksandra, Jelić-Ivanović, Zorana, Spasojević-Kalimanovska, Vesna, "Determination of non-cholesterol sterols in serum and HDL fraction by LC/MS-ms: Significance of matrix-related interferences" in Journal of Medical Biochemistry, 39 (2020):299-308,
https://doi.org/10.2478/jomb-2019-0044 . .

TY  - JOUR
AU  - Vladimirov, Sandra
AU  - Zeljković, Aleksandra
AU  - Gojković, Tamara
AU  - Miljković, Milica
AU  - Stefanović, Aleksandra
AU  - Zeljković, Dejan
AU  - Trifunović, Bratislav
AU  - Spasojević-Kalimanovska, Vesna
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3623
AB  - Background:Vitamin D deficiency is repeatedly reported in colorectal  cancer  (CRC).  Since  cholesterol  and  vitamin  D share common precursor 7-dehydrocholesterol (7-DHC), it would be important to explore the associations of key vitamin D metabolites and serum lipid parameters in patients with high and low grade CRC. The aim of this study was toanalyze    relationships    between    serum    25(OH)D3,24,25(OH)2D3  and  7-DHC  levels  and  serum  lipids  inpatients with CRC, and to evaluate their potential for prediction of risk for development of high grade CRC. Methods:We  recruited  82  patients  CRC  and  77  controls.7-DHC, 25(OH)D3 and 24,25(OH)2D3 were quantified by LC-MS/MS methods.Results:7-DHC, 25(OH)D3 and vitamin D metabolic ratio(VDMR)  were  significantly  lower  in  CRC  patients  than  in control   group   (P<0.001,   P<0.010,   P<0.050   and P<0.050,  respectively).  25(OH)D3  levels  were  higher  inpatients  with  grade  I  CRC  when  compared  to  grade  II(P<0.050). All vitamin D metabolites positively correlated with  total  cholesterol  (TC)  concentration  in  CRC  patients.25(OH)D3 was significant predictor of increased CRC risk(P<0.010).   After   adjustment   for   TC   concentration,25(OH)D3 lost its predictive abilities. However, 25(OH)D3 remained significant predictor of poorly differentiated type of cancer (P<0.050). Conclusions:We  found  significant  positive  association between  vitamin  D  status  and  serum  total  cholesterol.Although low 25(OH)D3 was found to be a significant risk factor for CRC development, the obtained results primarily suggest profound impact of cholesterol level on vitamin D status  in  CRC.  However,  our  results  suggest  that  low 25(OH)D3 might independently contribute to development of poorly differentiated tumor.
AB  - Uvod: Deficijencija vitamina D je učestalo javlja kod obolelih od kolorektalnog karcinoma (CRC). Kako holesterol i vitamin D dele zajednički prekursor 7-dehidroholesterol (7-DHC), bilo bi značajno ispitati povezanost ključnih metabolita vitamin D i serumskih lipidnih parametara kod pacijenata sa CRC klasifikovanih na osnovu gradusa. U ovom radu ispitivali smo odnos između serumskih koncentracija 25(OH)D3, 24,25(OH)2D3 and 7-DHC i serumskih lipida kod pacijenata sa CRC, kao i doprinos metabolita vitamina D u predikciji rizika za nastanak CRC visokog gradusa. Metode: U studiji su učestvovala 82 pacijenta sa CRC i 77 zdravih ispitanika. 7-DHC, 25(OH)D3 i 24,25(OH)2D3 su kvantifikovani pomoću metode LC-MS/MS. Rezultati: 7-DHC, 25(OH)D3 i odnos metabolita vitamina D (VDMR) su bili značajno niži kod pacijenata sa CRC u odnosu na kontrolnu grupu (P<0,001, P<0,010, P<0,050 i P<0,050, redom). Koncentracije 25(OH)D3 su bile više kod pacijenata sa gradusom I u odnosu na pacijente sa gradusom II (P<0,050). Svi metaboliti vitamina D su pozitivno korelirali sa ukupnim holesterolom kod pacijenata sa CRC. 25(OH)D3 je bio značajan prediktor povećanog rizika za nastanak CRC (P<0,010). Nakon korekcije za koncentraciju ukupnog holesterola, 25(OH)D3 je izgubio prediktivni značaj. Međutim, 25(OH)D3 je ostao značajan prediktor slabo diferenciranog karcinoma (P<0,050). Zaključak: Utvrđena je značajna pozitivna korelacija iz među statusa vitamina D i serumskih koncentracija ukupnog holesterola. Iako je uočeno da je nizak 25(OH)D3 značajan faktor rizika za razvoj CRC, naši rezultati upućuju na dominantan uticaj holesterola na status vitamina D kod ovih pacijenata. Ipak, uočeno je da niski nivoi 25(OH)D3 mogu nezavisno doprineti razvoju slabo diferentovanih oblika CRC.
PB  - Beograd : Društvo medicinskih biohemičara Srbije
T2  - Journal of Medical Biochemistry
T1  - Associations of cholesterol and vitamin D metabolites with the risk for development of high grade colorectal cancer
T1  - Povezanost metabolita holesterola i vitamina D sa rizikom za nastanak kolorektalnog karcinoma visokog gradusa
VL  - 39
IS  - 3
SP  - 318
EP  - 327
DO  - 10.2478/jomb-2019-0047
ER  - 

@article{
author = "Vladimirov, Sandra and Zeljković, Aleksandra and Gojković, Tamara and Miljković, Milica and Stefanović, Aleksandra and Zeljković, Dejan and Trifunović, Bratislav and Spasojević-Kalimanovska, Vesna",
year = "2020",
abstract = "Background:Vitamin D deficiency is repeatedly reported in colorectal  cancer  (CRC).  Since  cholesterol  and  vitamin  D share common precursor 7-dehydrocholesterol (7-DHC), it would be important to explore the associations of key vitamin D metabolites and serum lipid parameters in patients with high and low grade CRC. The aim of this study was toanalyze    relationships    between    serum    25(OH)D3,24,25(OH)2D3  and  7-DHC  levels  and  serum  lipids  inpatients with CRC, and to evaluate their potential for prediction of risk for development of high grade CRC. Methods:We  recruited  82  patients  CRC  and  77  controls.7-DHC, 25(OH)D3 and 24,25(OH)2D3 were quantified by LC-MS/MS methods.Results:7-DHC, 25(OH)D3 and vitamin D metabolic ratio(VDMR)  were  significantly  lower  in  CRC  patients  than  in control   group   (P<0.001,   P<0.010,   P<0.050   and P<0.050,  respectively).  25(OH)D3  levels  were  higher  inpatients  with  grade  I  CRC  when  compared  to  grade  II(P<0.050). All vitamin D metabolites positively correlated with  total  cholesterol  (TC)  concentration  in  CRC  patients.25(OH)D3 was significant predictor of increased CRC risk(P<0.010).   After   adjustment   for   TC   concentration,25(OH)D3 lost its predictive abilities. However, 25(OH)D3 remained significant predictor of poorly differentiated type of cancer (P<0.050). Conclusions:We  found  significant  positive  association between  vitamin  D  status  and  serum  total  cholesterol.Although low 25(OH)D3 was found to be a significant risk factor for CRC development, the obtained results primarily suggest profound impact of cholesterol level on vitamin D status  in  CRC.  However,  our  results  suggest  that  low 25(OH)D3 might independently contribute to development of poorly differentiated tumor., Uvod: Deficijencija vitamina D je učestalo javlja kod obolelih od kolorektalnog karcinoma (CRC). Kako holesterol i vitamin D dele zajednički prekursor 7-dehidroholesterol (7-DHC), bilo bi značajno ispitati povezanost ključnih metabolita vitamin D i serumskih lipidnih parametara kod pacijenata sa CRC klasifikovanih na osnovu gradusa. U ovom radu ispitivali smo odnos između serumskih koncentracija 25(OH)D3, 24,25(OH)2D3 and 7-DHC i serumskih lipida kod pacijenata sa CRC, kao i doprinos metabolita vitamina D u predikciji rizika za nastanak CRC visokog gradusa. Metode: U studiji su učestvovala 82 pacijenta sa CRC i 77 zdravih ispitanika. 7-DHC, 25(OH)D3 i 24,25(OH)2D3 su kvantifikovani pomoću metode LC-MS/MS. Rezultati: 7-DHC, 25(OH)D3 i odnos metabolita vitamina D (VDMR) su bili značajno niži kod pacijenata sa CRC u odnosu na kontrolnu grupu (P<0,001, P<0,010, P<0,050 i P<0,050, redom). Koncentracije 25(OH)D3 su bile više kod pacijenata sa gradusom I u odnosu na pacijente sa gradusom II (P<0,050). Svi metaboliti vitamina D su pozitivno korelirali sa ukupnim holesterolom kod pacijenata sa CRC. 25(OH)D3 je bio značajan prediktor povećanog rizika za nastanak CRC (P<0,010). Nakon korekcije za koncentraciju ukupnog holesterola, 25(OH)D3 je izgubio prediktivni značaj. Međutim, 25(OH)D3 je ostao značajan prediktor slabo diferenciranog karcinoma (P<0,050). Zaključak: Utvrđena je značajna pozitivna korelacija iz među statusa vitamina D i serumskih koncentracija ukupnog holesterola. Iako je uočeno da je nizak 25(OH)D3 značajan faktor rizika za razvoj CRC, naši rezultati upućuju na dominantan uticaj holesterola na status vitamina D kod ovih pacijenata. Ipak, uočeno je da niski nivoi 25(OH)D3 mogu nezavisno doprineti razvoju slabo diferentovanih oblika CRC.",
publisher = "Beograd : Društvo medicinskih biohemičara Srbije",
journal = "Journal of Medical Biochemistry",
title = "Associations of cholesterol and vitamin D metabolites with the risk for development of high grade colorectal cancer, Povezanost metabolita holesterola i vitamina D sa rizikom za nastanak kolorektalnog karcinoma visokog gradusa",
volume = "39",
number = "3",
pages = "318-327",
doi = "10.2478/jomb-2019-0047"
}

Vladimirov, S., Zeljković, A., Gojković, T., Miljković, M., Stefanović, A., Zeljković, D., Trifunović, B.,& Spasojević-Kalimanovska, V.. (2020). Associations of cholesterol and vitamin D metabolites with the risk for development of high grade colorectal cancer. in Journal of Medical Biochemistry
Beograd : Društvo medicinskih biohemičara Srbije., 39(3), 318-327.
https://doi.org/10.2478/jomb-2019-0047

Vladimirov S, Zeljković A, Gojković T, Miljković M, Stefanović A, Zeljković D, Trifunović B, Spasojević-Kalimanovska V. Associations of cholesterol and vitamin D metabolites with the risk for development of high grade colorectal cancer. in Journal of Medical Biochemistry. 2020;39(3):318-327.
doi:10.2478/jomb-2019-0047 .

Vladimirov, Sandra, Zeljković, Aleksandra, Gojković, Tamara, Miljković, Milica, Stefanović, Aleksandra, Zeljković, Dejan, Trifunović, Bratislav, Spasojević-Kalimanovska, Vesna, "Associations of cholesterol and vitamin D metabolites with the risk for development of high grade colorectal cancer" in Journal of Medical Biochemistry, 39, no. 3 (2020):318-327,
https://doi.org/10.2478/jomb-2019-0047 . .