TY  - JOUR
AU  - Amidžić, Rada
AU  - Brborić, Jasmina
AU  - Čudina, Olivera
AU  - Vladimirov, Sote
PY  - 2005
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/656
AB  - A simple and sensitive reversed-phase, ion-pair HPLC method was developed and validated for the simultaneous determination of B-group vitamins, thiamine chloride hydrochloride (B1), nicotinamide (B3), pyridoxine hydrochloride (B6) and folic acid in Pentovit® coated tablets. The cyanocobalamine (B12) was determined separately, because of its low concentration in the investigated multivitamin preparation. RP-HPLC analysis was per- formed with a LKB 2150 HPLC system, equipped with a UV/VIS Waters M 484 detector. The procedures for the determination of B1, B2, B6 and folic acid were carried out on a Supelcosil ABZ+ (15 cm 4.6 mm; 5 m) column with methanol-5mM heptanesulphonic acid sodium salt 0.1 % triethylamine TEA (25:75 V/V); pH 2.8 as the mobile phase. For the determination of B12 a Suplex pKb-100 (15 cm 4.6 mm; 5 m) column and methanol–water (22:78 V/V) as the mobile phase were used. The column effluents were monitored at 290 nm for B1, B3, B6 and folic acid, and at 550 nm for B12. The obtained results and statistical parameters for all the investigated vitamins of the B-group in Pentovit® coated tablets were satisfactory and ranged from 90.4 % to 108.5 % (RSD. from 0.5 % to 4.1 %). The parameters for the validation of the methods are given.
AB  - Za istovremeno određivanje vitamina B-kompleksa, tiamin-hlorida-hidrohlorida (B1), nikotinamida (B3), piridoksin-hidrohlorida (B6) i folne kiseline u Pentovit® tabletama primenjena je i validirana jednostavna i osetqiva metoda reverzno-fazne jon-par tečne hromatografije pod visokim pritiskom. Zbog niske koncentracije u ispitivanom preparatu cijanokobalamin (B12) je određen pod drugim uslovima. Određivanje vitamina B1, B3, B6 i folne kiseline izvedeno je na LKB 2150 HPLC sistemu, sa UV/VIS Waters M 484 detektorom, korišćewem Supelcosil ABZ+ kolone (15 cm x 4,6 mm; 5 _m) sa mobilnom fazom metanol – 5 mM natrijum-heptansulfonat/0,1 % trietilamin (25:75 V/V) pri pH 2,8. Za određivanje vitamina B12 korišćena je kolona Suplex pKb-100 (15 cm x 4,6 mm; 5 nm) i mobilna faza metanol–voda (22:78 V/V). Vitamini B1, B3, B6 i folna kiselina detektovani su na 290 nm, a vitamin B12 na 550nm. Dobijene vrednosti sadržaja i statistički parametri za sve vitamine B-kompleksa u Pentovit® tabletama su zadovoljavajući i kreću se u opsegu od 90,4%do 108,5% (RSD od 0,5%do 4,1 %). Prikazani su i parametri za validaciju metode.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - RP-HPLC determination of vitamins, folic acid and B12 in multivitamin tablets
T1  - RP-HPLC određivanje vitamina B1, B3, B6, folne kiseline i B12 multivitaminskih tableta
VL  - 70
IS  - 10
SP  - 1229
EP  - 1235
DO  - 10.2298/JSC0510229A
ER  - 

@article{
author = "Amidžić, Rada and Brborić, Jasmina and Čudina, Olivera and Vladimirov, Sote",
year = "2005",
abstract = "A simple and sensitive reversed-phase, ion-pair HPLC method was developed and validated for the simultaneous determination of B-group vitamins, thiamine chloride hydrochloride (B1), nicotinamide (B3), pyridoxine hydrochloride (B6) and folic acid in Pentovit® coated tablets. The cyanocobalamine (B12) was determined separately, because of its low concentration in the investigated multivitamin preparation. RP-HPLC analysis was per- formed with a LKB 2150 HPLC system, equipped with a UV/VIS Waters M 484 detector. The procedures for the determination of B1, B2, B6 and folic acid were carried out on a Supelcosil ABZ+ (15 cm 4.6 mm; 5 m) column with methanol-5mM heptanesulphonic acid sodium salt 0.1 % triethylamine TEA (25:75 V/V); pH 2.8 as the mobile phase. For the determination of B12 a Suplex pKb-100 (15 cm 4.6 mm; 5 m) column and methanol–water (22:78 V/V) as the mobile phase were used. The column effluents were monitored at 290 nm for B1, B3, B6 and folic acid, and at 550 nm for B12. The obtained results and statistical parameters for all the investigated vitamins of the B-group in Pentovit® coated tablets were satisfactory and ranged from 90.4 % to 108.5 % (RSD. from 0.5 % to 4.1 %). The parameters for the validation of the methods are given., Za istovremeno određivanje vitamina B-kompleksa, tiamin-hlorida-hidrohlorida (B1), nikotinamida (B3), piridoksin-hidrohlorida (B6) i folne kiseline u Pentovit® tabletama primenjena je i validirana jednostavna i osetqiva metoda reverzno-fazne jon-par tečne hromatografije pod visokim pritiskom. Zbog niske koncentracije u ispitivanom preparatu cijanokobalamin (B12) je određen pod drugim uslovima. Određivanje vitamina B1, B3, B6 i folne kiseline izvedeno je na LKB 2150 HPLC sistemu, sa UV/VIS Waters M 484 detektorom, korišćewem Supelcosil ABZ+ kolone (15 cm x 4,6 mm; 5 _m) sa mobilnom fazom metanol – 5 mM natrijum-heptansulfonat/0,1 % trietilamin (25:75 V/V) pri pH 2,8. Za određivanje vitamina B12 korišćena je kolona Suplex pKb-100 (15 cm x 4,6 mm; 5 nm) i mobilna faza metanol–voda (22:78 V/V). Vitamini B1, B3, B6 i folna kiselina detektovani su na 290 nm, a vitamin B12 na 550nm. Dobijene vrednosti sadržaja i statistički parametri za sve vitamine B-kompleksa u Pentovit® tabletama su zadovoljavajući i kreću se u opsegu od 90,4%do 108,5% (RSD od 0,5%do 4,1 %). Prikazani su i parametri za validaciju metode.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "RP-HPLC determination of vitamins, folic acid and B12 in multivitamin tablets, RP-HPLC određivanje vitamina B1, B3, B6, folne kiseline i B12 multivitaminskih tableta",
volume = "70",
number = "10",
pages = "1229-1235",
doi = "10.2298/JSC0510229A"
}

Amidžić, R., Brborić, J., Čudina, O.,& Vladimirov, S.. (2005). RP-HPLC determination of vitamins, folic acid and B12 in multivitamin tablets. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 70(10), 1229-1235.
https://doi.org/10.2298/JSC0510229A

Amidžić R, Brborić J, Čudina O, Vladimirov S. RP-HPLC determination of vitamins, folic acid and B12 in multivitamin tablets. in Journal of the Serbian Chemical Society. 2005;70(10):1229-1235.
doi:10.2298/JSC0510229A .

Amidžić, Rada, Brborić, Jasmina, Čudina, Olivera, Vladimirov, Sote, "RP-HPLC determination of vitamins, folic acid and B12 in multivitamin tablets" in Journal of the Serbian Chemical Society, 70, no. 10 (2005):1229-1235,
https://doi.org/10.2298/JSC0510229A . .

TY  - JOUR
AU  - Kuntić, Vesna
AU  - Pejić, Nataša
AU  - Mićić, Svetlana
AU  - Vukojević, Vladana
AU  - Vujić, Zorica
AU  - Malešev, Dušan
PY  - 2005
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/657
AB  - Asimple, rapid and accurate procedure for the quantitative determination of quercetin in its pure form and in formulations has been developed. The method is based on the spectrophotometric determination of a complex formed between quercetin and potassium titanyloxalate in 50 % ethanolic solutions. To characterize the quercetin titanyloxalato complex, the stability constants of the complex were determinated potentiometrically and spectrophotometrically at different temperatures (T = 26.0oC, 34oC and 39.0oC), as well as at different ionic strengths (I = 5.0x10-4 mol dm-3, 3.0x10-2 mol dm-3 and 6.0x10-2 mol dm-3) and the thermodynamic parameters were calculated. As quercetin is usually conjugated to vitamin C in pharmaceutical formulations, two procedures for the quantitative determination of quercetin by this complexing reaction were tested - both in the absence and presence of ascorbic acid. In both procedures, the Beer law was obeyed over the same concentration range of quercetin, i.e., 0.85 μg mL-1 - 16.9 μg mL-1. In the first procedure in the absence of ascorbic acid the molar absorptivity coefficient of the quercetin-titanyloxalate complex is a=2.49 x 104 mol-1 dm3 cm-1, Sandells sensitivity of the method is S = 1.35 x 10-2 μg cm-2 and the detection limit is d = 0.67 μg mL-1. Whereas, in the presence of ascorbic acid (second procedure) a = 3.04 x 104 mol-1 dm3 cm-1, S = 1.11 x 10-2 μg mL-1. The proposed method was verified for the determination of quercetin in pharmaceutical dosage forms.
AB  - Predložen je brz, jednostavan i tačan metod za određivanje kvercetina u farmaceutskim sirovinama i doziranim oblicima.Metod je baziran na spektrofotometrijskom određivanju kompleksa formiranog između kvercetina i kalijum-titaniloksalata u 50 % etanolu. Određene su konstante stabilnosti kvercetin titaniloksalatnog kompleksa potenciometrijski i spektrofotometrijski na različitim temperaturama (T = 26.0oC, 34oC and 39.0oC) i jonskim jačinama (I = 5.0x10-4 mol dm-3, 3.0x10-2 mol dm-3 and 6.0x10-2 mol dm-3) i izračunati su termodinamički parametri. Kako se kvercetin uobičajeno nalazi zajedno sa vitaminom C u doziranim oblicima, predložene su dve procedure za određivanje kvercetina: bez i u prisustvu askorbinske kiseline.U obe predložene procedure, Beer-ov zakon važi u oblasti 00.85 μg mL-1 - 16.9 μg mL-1 kvercetina. Prema prvoj proceduri, molarni apsorpcioni koeficijent kvercetin–titaniloksalatnog kompleksa je a=2.49 x 104 mol-1 dm3 cm-1, Sandelova osetljivost metode je S = 1.35 x 10-2 μg cm-2, a limit detekcije je d = 0.67 μg mL-1. Prema drugoj proceduri, a = 3.04 x 104 mol-1 dm3 cm-1, S = 1.11 x 10-2 μg mL-1. Predloženi metod je primenjen za određivanje kvercetina u doziranim oblicima.
PB  - Srpsko hemijsko društvo, Beograd
T2  - Journal of the Serbian Chemical Society
T1  - Determination of quercetin in pharmaceutical formulations via its reaction with potassium-titanyloxalate: Determination of the stability constants of the quercetin-titanyloxalato complex
T1  - Određivanje kvercetina u farmaceutskim preparatima preko reakcije sa kalijum-titaniloksalatom - određivanje konstanti stabilnosti kvercetin-titaniloksalatnog kompleksa
VL  - 70
IS  - 5
SP  - 753
EP  - 763
DO  - 10.2298/JSC0505753K
ER  - 

@article{
author = "Kuntić, Vesna and Pejić, Nataša and Mićić, Svetlana and Vukojević, Vladana and Vujić, Zorica and Malešev, Dušan",
year = "2005",
abstract = "Asimple, rapid and accurate procedure for the quantitative determination of quercetin in its pure form and in formulations has been developed. The method is based on the spectrophotometric determination of a complex formed between quercetin and potassium titanyloxalate in 50 % ethanolic solutions. To characterize the quercetin titanyloxalato complex, the stability constants of the complex were determinated potentiometrically and spectrophotometrically at different temperatures (T = 26.0oC, 34oC and 39.0oC), as well as at different ionic strengths (I = 5.0x10-4 mol dm-3, 3.0x10-2 mol dm-3 and 6.0x10-2 mol dm-3) and the thermodynamic parameters were calculated. As quercetin is usually conjugated to vitamin C in pharmaceutical formulations, two procedures for the quantitative determination of quercetin by this complexing reaction were tested - both in the absence and presence of ascorbic acid. In both procedures, the Beer law was obeyed over the same concentration range of quercetin, i.e., 0.85 μg mL-1 - 16.9 μg mL-1. In the first procedure in the absence of ascorbic acid the molar absorptivity coefficient of the quercetin-titanyloxalate complex is a=2.49 x 104 mol-1 dm3 cm-1, Sandells sensitivity of the method is S = 1.35 x 10-2 μg cm-2 and the detection limit is d = 0.67 μg mL-1. Whereas, in the presence of ascorbic acid (second procedure) a = 3.04 x 104 mol-1 dm3 cm-1, S = 1.11 x 10-2 μg mL-1. The proposed method was verified for the determination of quercetin in pharmaceutical dosage forms., Predložen je brz, jednostavan i tačan metod za određivanje kvercetina u farmaceutskim sirovinama i doziranim oblicima.Metod je baziran na spektrofotometrijskom određivanju kompleksa formiranog između kvercetina i kalijum-titaniloksalata u 50 % etanolu. Određene su konstante stabilnosti kvercetin titaniloksalatnog kompleksa potenciometrijski i spektrofotometrijski na različitim temperaturama (T = 26.0oC, 34oC and 39.0oC) i jonskim jačinama (I = 5.0x10-4 mol dm-3, 3.0x10-2 mol dm-3 and 6.0x10-2 mol dm-3) i izračunati su termodinamički parametri. Kako se kvercetin uobičajeno nalazi zajedno sa vitaminom C u doziranim oblicima, predložene su dve procedure za određivanje kvercetina: bez i u prisustvu askorbinske kiseline.U obe predložene procedure, Beer-ov zakon važi u oblasti 00.85 μg mL-1 - 16.9 μg mL-1 kvercetina. Prema prvoj proceduri, molarni apsorpcioni koeficijent kvercetin–titaniloksalatnog kompleksa je a=2.49 x 104 mol-1 dm3 cm-1, Sandelova osetljivost metode je S = 1.35 x 10-2 μg cm-2, a limit detekcije je d = 0.67 μg mL-1. Prema drugoj proceduri, a = 3.04 x 104 mol-1 dm3 cm-1, S = 1.11 x 10-2 μg mL-1. Predloženi metod je primenjen za određivanje kvercetina u doziranim oblicima.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Determination of quercetin in pharmaceutical formulations via its reaction with potassium-titanyloxalate: Determination of the stability constants of the quercetin-titanyloxalato complex, Određivanje kvercetina u farmaceutskim preparatima preko reakcije sa kalijum-titaniloksalatom - određivanje konstanti stabilnosti kvercetin-titaniloksalatnog kompleksa",
volume = "70",
number = "5",
pages = "753-763",
doi = "10.2298/JSC0505753K"
}

Kuntić, V., Pejić, N., Mićić, S., Vukojević, V., Vujić, Z.,& Malešev, D.. (2005). Determination of quercetin in pharmaceutical formulations via its reaction with potassium-titanyloxalate: Determination of the stability constants of the quercetin-titanyloxalato complex. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 70(5), 753-763.
https://doi.org/10.2298/JSC0505753K

Kuntić V, Pejić N, Mićić S, Vukojević V, Vujić Z, Malešev D. Determination of quercetin in pharmaceutical formulations via its reaction with potassium-titanyloxalate: Determination of the stability constants of the quercetin-titanyloxalato complex. in Journal of the Serbian Chemical Society. 2005;70(5):753-763.
doi:10.2298/JSC0505753K .

Kuntić, Vesna, Pejić, Nataša, Mićić, Svetlana, Vukojević, Vladana, Vujić, Zorica, Malešev, Dušan, "Determination of quercetin in pharmaceutical formulations via its reaction with potassium-titanyloxalate: Determination of the stability constants of the quercetin-titanyloxalato complex" in Journal of the Serbian Chemical Society, 70, no. 5 (2005):753-763,
https://doi.org/10.2298/JSC0505753K . .