TY  - JOUR
AU  - Topalović, Dijana
AU  - Dekanski, Dragana
AU  - Potparević, Biljana
AU  - Pirković, Andrea
AU  - Borozan, Sunčica
AU  - Bajić, Vladan
AU  - Stojanović, Danilo
AU  - Giampieri, Francesca
AU  - Gasparrini, Massimiliano
AU  - Živković, Lada
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3140
AB  - Phenolic groups of steroidal or nonsteroidal estrogens can redox cycle, leading to oxidative stress, where creation of reactive oxygen species are recognized as the main mechanism of their DNA damage properties. Dry olive (Olea europaea L.) leaf extract is known to contain bioactive and antioxidative components and to have an ability to modulate the effects of various oxidants in cells. The main goal of this study was to investigate antigenotoxic potential of a standardized dry olive leaf extract on DNA damage induced by 17β-estradiol and diethylstilbestrol in human whole blood cells in vitro, using comet assay. Our results indicated that both hormones showed a genotoxic effect at a concentration of 100 μM (P  lt  0.05, n = 6). Dry olive leaf extract was efficient in reducing number of cells with estrogen-induced DNA damage at tested concentrations (0.125, 0.5 and 1 mg/mL) (P  lt  0.05, n = 6) and under two experimental protocols, pre-treatment and post-treatment, exhibiting antigenotoxic properties. Analysis of antioxidant properties of the extract revealed moderate ABTS radical scavenging properties and reducing power. Overall, our results suggested that the protective potential of dry olive leaf extract could arise from the synergistic effect of its scavenging activity and enhancement of the cells' antioxidant capacity.
PB  - Elsevier B.V.
T2  - Mutation Research - Genetic Toxicology and Environmental Mutagenesis
T1  - Dry olive leaf extract attenuates DNA damage induced by estradiol and diethylstilbestrol in human peripheral blood cells in vitro
VL  - 845
DO  - 10.1016/j.mrgentox.2018.12.001
ER  - 

@article{
author = "Topalović, Dijana and Dekanski, Dragana and Potparević, Biljana and Pirković, Andrea and Borozan, Sunčica and Bajić, Vladan and Stojanović, Danilo and Giampieri, Francesca and Gasparrini, Massimiliano and Živković, Lada",
year = "2019",
abstract = "Phenolic groups of steroidal or nonsteroidal estrogens can redox cycle, leading to oxidative stress, where creation of reactive oxygen species are recognized as the main mechanism of their DNA damage properties. Dry olive (Olea europaea L.) leaf extract is known to contain bioactive and antioxidative components and to have an ability to modulate the effects of various oxidants in cells. The main goal of this study was to investigate antigenotoxic potential of a standardized dry olive leaf extract on DNA damage induced by 17β-estradiol and diethylstilbestrol in human whole blood cells in vitro, using comet assay. Our results indicated that both hormones showed a genotoxic effect at a concentration of 100 μM (P  lt  0.05, n = 6). Dry olive leaf extract was efficient in reducing number of cells with estrogen-induced DNA damage at tested concentrations (0.125, 0.5 and 1 mg/mL) (P  lt  0.05, n = 6) and under two experimental protocols, pre-treatment and post-treatment, exhibiting antigenotoxic properties. Analysis of antioxidant properties of the extract revealed moderate ABTS radical scavenging properties and reducing power. Overall, our results suggested that the protective potential of dry olive leaf extract could arise from the synergistic effect of its scavenging activity and enhancement of the cells' antioxidant capacity.",
publisher = "Elsevier B.V.",
journal = "Mutation Research - Genetic Toxicology and Environmental Mutagenesis",
title = "Dry olive leaf extract attenuates DNA damage induced by estradiol and diethylstilbestrol in human peripheral blood cells in vitro",
volume = "845",
doi = "10.1016/j.mrgentox.2018.12.001"
}

Topalović, D., Dekanski, D., Potparević, B., Pirković, A., Borozan, S., Bajić, V., Stojanović, D., Giampieri, F., Gasparrini, M.,& Živković, L.. (2019). Dry olive leaf extract attenuates DNA damage induced by estradiol and diethylstilbestrol in human peripheral blood cells in vitro. in Mutation Research - Genetic Toxicology and Environmental Mutagenesis
Elsevier B.V.., 845.
https://doi.org/10.1016/j.mrgentox.2018.12.001

Topalović D, Dekanski D, Potparević B, Pirković A, Borozan S, Bajić V, Stojanović D, Giampieri F, Gasparrini M, Živković L. Dry olive leaf extract attenuates DNA damage induced by estradiol and diethylstilbestrol in human peripheral blood cells in vitro. in Mutation Research - Genetic Toxicology and Environmental Mutagenesis. 2019;845.
doi:10.1016/j.mrgentox.2018.12.001 .

Topalović, Dijana, Dekanski, Dragana, Potparević, Biljana, Pirković, Andrea, Borozan, Sunčica, Bajić, Vladan, Stojanović, Danilo, Giampieri, Francesca, Gasparrini, Massimiliano, Živković, Lada, "Dry olive leaf extract attenuates DNA damage induced by estradiol and diethylstilbestrol in human peripheral blood cells in vitro" in Mutation Research - Genetic Toxicology and Environmental Mutagenesis, 845 (2019),
https://doi.org/10.1016/j.mrgentox.2018.12.001 . .

TY  - JOUR
AU  - Janković, Milena
AU  - Živković, Lada
AU  - Pirković, Andrea
AU  - Topalović, Dijana
AU  - Dekanski, Dragana
AU  - Bajić, Vladan
AU  - Potparević, Biljana
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2941
AB  - Aim. Oxidative stress is a consequence of the increased production of free radicals that is caused by the disturbance in the balance of oxidation-reduction activity. Salvianolic acid B is a polyphenol compound, derived from the plant Salvia miltiorrhiza Bunge, which showed significant antioxidant properties. The aim of this study is the investigation of genotoxic potential of salvianolic acid B and evaluation of its antigenotoxic activity against the DNA damage induced by hydrogen peroxide in peripheral blood leukocytes in vitro, using the alkaline Comet assay. Materials and Methods. The evaluation of the ability of various concentrations of salvianolic acid B (12.5μM, 25μM and 50 μM) to reduce the number of cells with DNA damage caused by hydrogen peroxide as an oxidant was performed under two experimental protocols: pretreatment and cotreatment, in order to determine antigenotoxicity on preventive and intervention levels. Results. Results indicate that the salvianolic acid B did not exhibit a genotoxic effect after 30 minutes of incubation, in the tested concentrations. In the pretreatment, a concentration of 50 μM showed a significant decrease of the hydrogen peroxide induced DNA damage. Salvianolic acid B was more effective in reducing DNA damage in cotreatment, where concentrations of 25 μM and 50 μM demonstrated a significant abrogation of DNA damage. Protective effect of salvianolic acid B was dependent on the concentration. Conclusions. The results showed that salvianolic acid B has pronounced antigenotoxic effect on the intervention level, which makes it a potential agent in treatment of diseases in which oxidative DNA damage plays an important role.
AB  - Cilj. Oksidativni stres je posledica prekomerne produkcije slobodnih radikala i nastaje usled poremećaja u ravnoteži oksido-redukcionih procesa. Salvianolična kiselina B je polifenolno jedinjenje, poreklom iz biljke Salvia miltiorrhiza Bunge, koje je pokazalo značajna antioksidativna svojstva. Cilj rada bio je da se ispita genotoksični potencijal salvianolične kiseline B i izvrši evaluacija njene antigenotoksične aktivnosti na DNK oštećenja indukovana vodonik-peroksidom u leukocitima periferne krvi in vitro, primenom alkalnog Komet testa. Materijal i metode. Procenjena je sposobnost različitih koncentracija salvianolične kiseline B (12,5 μM, 25 μM i 50 μM) da redukuju broj ćelija sa DNK oštećenjem izazvanim vodonik-peroksidom kao oksidansom u okviru dva eksperimentalna protokola: pretretmana i kotretmana radi utvrđivanja antigenotoksičnosti na preventivnom i interventnom nivou. Rezultati. Rezultati su pokazali da salvianolična kiselina B, nakon 30 minuta inkubacije, nije ispoljila genotoksičan efekat u ispitivanim koncentracijama. U pretretmanu, koncentracija od 50 μM pokazala je značajnu redukciju DNK oštećenja indukovanih vodonik-peroksidom. Salvianolična kiselina B bila je efikasnija u redukciji DNK oštećenja u kotretmanu, gde su koncentracije od 25 μM i 50 μM pokazale značajan efekat redukcije nivoa DNK oštećenja. Pokazani protektivni efekat salvianolične kiseline B zavisio je od koncentracije. Zaključak. Dobijeni rezultati pokazali su da salvianolična kiselina B ima izraženiji antigenotoksični efekat na interventnom nivou, što je čini potencijalnim agensom za primenu kod oboljenja u kojima oksidativna DNK oštećenja imaju važnu ulogu.
PB  - Srpsko lekarsko društvo - Okružna podružnica Kragujevac, Kragujevac
T2  - Medicinski časopis
T1  - Evaluation of antigenotoxic potential of salvianolic acid B with hydrogen peroxide on human peripheral blood leukocytes in vitro
T1  - Evaluacija antigenotoksičnog potencijala salvianolične kiseline B u prisustvu vodonik-peroksida na leukocitima periferne krvi in vitro
VL  - 51
IS  - 2
SP  - 39
EP  - 45
DO  - 10.5937/mckg51-15559
ER  - 

@article{
author = "Janković, Milena and Živković, Lada and Pirković, Andrea and Topalović, Dijana and Dekanski, Dragana and Bajić, Vladan and Potparević, Biljana",
year = "2017",
abstract = "Aim. Oxidative stress is a consequence of the increased production of free radicals that is caused by the disturbance in the balance of oxidation-reduction activity. Salvianolic acid B is a polyphenol compound, derived from the plant Salvia miltiorrhiza Bunge, which showed significant antioxidant properties. The aim of this study is the investigation of genotoxic potential of salvianolic acid B and evaluation of its antigenotoxic activity against the DNA damage induced by hydrogen peroxide in peripheral blood leukocytes in vitro, using the alkaline Comet assay. Materials and Methods. The evaluation of the ability of various concentrations of salvianolic acid B (12.5μM, 25μM and 50 μM) to reduce the number of cells with DNA damage caused by hydrogen peroxide as an oxidant was performed under two experimental protocols: pretreatment and cotreatment, in order to determine antigenotoxicity on preventive and intervention levels. Results. Results indicate that the salvianolic acid B did not exhibit a genotoxic effect after 30 minutes of incubation, in the tested concentrations. In the pretreatment, a concentration of 50 μM showed a significant decrease of the hydrogen peroxide induced DNA damage. Salvianolic acid B was more effective in reducing DNA damage in cotreatment, where concentrations of 25 μM and 50 μM demonstrated a significant abrogation of DNA damage. Protective effect of salvianolic acid B was dependent on the concentration. Conclusions. The results showed that salvianolic acid B has pronounced antigenotoxic effect on the intervention level, which makes it a potential agent in treatment of diseases in which oxidative DNA damage plays an important role., Cilj. Oksidativni stres je posledica prekomerne produkcije slobodnih radikala i nastaje usled poremećaja u ravnoteži oksido-redukcionih procesa. Salvianolična kiselina B je polifenolno jedinjenje, poreklom iz biljke Salvia miltiorrhiza Bunge, koje je pokazalo značajna antioksidativna svojstva. Cilj rada bio je da se ispita genotoksični potencijal salvianolične kiseline B i izvrši evaluacija njene antigenotoksične aktivnosti na DNK oštećenja indukovana vodonik-peroksidom u leukocitima periferne krvi in vitro, primenom alkalnog Komet testa. Materijal i metode. Procenjena je sposobnost različitih koncentracija salvianolične kiseline B (12,5 μM, 25 μM i 50 μM) da redukuju broj ćelija sa DNK oštećenjem izazvanim vodonik-peroksidom kao oksidansom u okviru dva eksperimentalna protokola: pretretmana i kotretmana radi utvrđivanja antigenotoksičnosti na preventivnom i interventnom nivou. Rezultati. Rezultati su pokazali da salvianolična kiselina B, nakon 30 minuta inkubacije, nije ispoljila genotoksičan efekat u ispitivanim koncentracijama. U pretretmanu, koncentracija od 50 μM pokazala je značajnu redukciju DNK oštećenja indukovanih vodonik-peroksidom. Salvianolična kiselina B bila je efikasnija u redukciji DNK oštećenja u kotretmanu, gde su koncentracije od 25 μM i 50 μM pokazale značajan efekat redukcije nivoa DNK oštećenja. Pokazani protektivni efekat salvianolične kiseline B zavisio je od koncentracije. Zaključak. Dobijeni rezultati pokazali su da salvianolična kiselina B ima izraženiji antigenotoksični efekat na interventnom nivou, što je čini potencijalnim agensom za primenu kod oboljenja u kojima oksidativna DNK oštećenja imaju važnu ulogu.",
publisher = "Srpsko lekarsko društvo - Okružna podružnica Kragujevac, Kragujevac",
journal = "Medicinski časopis",
title = "Evaluation of antigenotoxic potential of salvianolic acid B with hydrogen peroxide on human peripheral blood leukocytes in vitro, Evaluacija antigenotoksičnog potencijala salvianolične kiseline B u prisustvu vodonik-peroksida na leukocitima periferne krvi in vitro",
volume = "51",
number = "2",
pages = "39-45",
doi = "10.5937/mckg51-15559"
}

Janković, M., Živković, L., Pirković, A., Topalović, D., Dekanski, D., Bajić, V.,& Potparević, B.. (2017). Evaluation of antigenotoxic potential of salvianolic acid B with hydrogen peroxide on human peripheral blood leukocytes in vitro. in Medicinski časopis
Srpsko lekarsko društvo - Okružna podružnica Kragujevac, Kragujevac., 51(2), 39-45.
https://doi.org/10.5937/mckg51-15559

Janković M, Živković L, Pirković A, Topalović D, Dekanski D, Bajić V, Potparević B. Evaluation of antigenotoxic potential of salvianolic acid B with hydrogen peroxide on human peripheral blood leukocytes in vitro. in Medicinski časopis. 2017;51(2):39-45.
doi:10.5937/mckg51-15559 .

Janković, Milena, Živković, Lada, Pirković, Andrea, Topalović, Dijana, Dekanski, Dragana, Bajić, Vladan, Potparević, Biljana, "Evaluation of antigenotoxic potential of salvianolic acid B with hydrogen peroxide on human peripheral blood leukocytes in vitro" in Medicinski časopis, 51, no. 2 (2017):39-45,
https://doi.org/10.5937/mckg51-15559 . .