TY  - JOUR
AU  - Živković, Lada
AU  - Akar, Banu
AU  - Roux, Brianna M.
AU  - Potparević, Biljana
AU  - Bajić, Vladan
AU  - Brey, Eric M.
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2934
AB  - Poly (lactic-co-glycolic acid) (PLGA)-based materials are widely investigated for drug delivery and tissue engineering applications. Despite their popularity the genotoxic potential of PLGA has not been investigated. In this study, the comet assay, a sensitive assay for DNA damage, was used to evaluate potential genotoxicity in model cell types exposed to PLGA microspheres. Human umbilical vein endothelial cells (HUVECs) and mesenchymal stem cells (MSCs) cells were exposed to PLGA microspheres (0.4-6mg/mL) and DNA damage assessed at 24h, 4days, and 7days. DNA damage was not identified after 24h. However, after 4 and 7 days of exposure to 2 and 6mg/mL of PLGA microspheres a significant elevation of DNA damage in both cell types was observed. The PLGA microspheres did not exhibit any cytotoxic effects on the cells under the conditions tested. Our results suggest that PLGA may have a genotoxic effect on cells. A broader investigation of the PLGA genotoxic profile in biological systems is needed.
PB  - Wiley-Blackwell, Hoboken
T2  - Journal of Biomedical Materials Research Part A
T1  - Investigation of DNA damage in cells exposed to poly (lactic-co-glycolic acid) microspheres
VL  - 105
IS  - 1
SP  - 284
EP  - 291
DO  - 10.1002/jbm.a.35849
ER  - 

@article{
author = "Živković, Lada and Akar, Banu and Roux, Brianna M. and Potparević, Biljana and Bajić, Vladan and Brey, Eric M.",
year = "2017",
abstract = "Poly (lactic-co-glycolic acid) (PLGA)-based materials are widely investigated for drug delivery and tissue engineering applications. Despite their popularity the genotoxic potential of PLGA has not been investigated. In this study, the comet assay, a sensitive assay for DNA damage, was used to evaluate potential genotoxicity in model cell types exposed to PLGA microspheres. Human umbilical vein endothelial cells (HUVECs) and mesenchymal stem cells (MSCs) cells were exposed to PLGA microspheres (0.4-6mg/mL) and DNA damage assessed at 24h, 4days, and 7days. DNA damage was not identified after 24h. However, after 4 and 7 days of exposure to 2 and 6mg/mL of PLGA microspheres a significant elevation of DNA damage in both cell types was observed. The PLGA microspheres did not exhibit any cytotoxic effects on the cells under the conditions tested. Our results suggest that PLGA may have a genotoxic effect on cells. A broader investigation of the PLGA genotoxic profile in biological systems is needed.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "Journal of Biomedical Materials Research Part A",
title = "Investigation of DNA damage in cells exposed to poly (lactic-co-glycolic acid) microspheres",
volume = "105",
number = "1",
pages = "284-291",
doi = "10.1002/jbm.a.35849"
}

Živković, L., Akar, B., Roux, B. M., Potparević, B., Bajić, V.,& Brey, E. M.. (2017). Investigation of DNA damage in cells exposed to poly (lactic-co-glycolic acid) microspheres. in Journal of Biomedical Materials Research Part A
Wiley-Blackwell, Hoboken., 105(1), 284-291.
https://doi.org/10.1002/jbm.a.35849

Živković L, Akar B, Roux BM, Potparević B, Bajić V, Brey EM. Investigation of DNA damage in cells exposed to poly (lactic-co-glycolic acid) microspheres. in Journal of Biomedical Materials Research Part A. 2017;105(1):284-291.
doi:10.1002/jbm.a.35849 .

Živković, Lada, Akar, Banu, Roux, Brianna M., Potparević, Biljana, Bajić, Vladan, Brey, Eric M., "Investigation of DNA damage in cells exposed to poly (lactic-co-glycolic acid) microspheres" in Journal of Biomedical Materials Research Part A, 105, no. 1 (2017):284-291,
https://doi.org/10.1002/jbm.a.35849 . .