TY  - JOUR
AU  - Stanisavljević, Nataša
AU  - Stojanovich, Ljudmila
AU  - Đoković, Aleksandra
AU  - Todić, Brankica
AU  - Dopsaj, Violeta
AU  - Saponjski, Jovica
AU  - Saponjski, Dušan
AU  - Marković, Olivera
AU  - Belizna, Cristina
AU  - Zdravković, Marija
AU  - Marisavljević, Dragomir
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4310
AB  - Objective: The potential contribution of asymmetric dimethylarginine (ADMA) and high-sensitivity C reactive protein (hsCRP) to endothelial dysfunction in APS patients has not been studied in detail, until now. The study involved 105 APS patients (59 diagnosed with primary APS (PAPS) and 46 APS associated with systemic lupus erythematosus (SAPS)) who were compared to 40 controls. Endothelial dysfunction was assessed by measurement of flow-mediated dilatation (FMD) and glyceryl trinitrate dilatation (NMD) of the brachial artery. ADMA (micromol/L) was analyzed by ELISA. Results: FMD in patients with APS was significantly lower than that of the controls (p < 0.001), with no difference between the PAPS and the SAPS groups. ADMA and hsCRP concentrations were significantly higher in the patient cohort than in the control group (p < 0.001, p = 0.006, respectively), as was the case with the SAPS group as compared to the PAPS group (p < 0.001, p = 0.022, respectively). FMD impairment correlated to ADMA (ρ 0.472, p < 0.001) and to hsCRP (ρ 0.181, p = 0.033). In the regression model, the ADMA concentration confirmed the strength of its association (B 0.518, SE 0.183, Wald 8.041, p = 0.005, Exp(B) 1.679, 95% CI 1.174–2.402) to FMD impairment. The synergistic probability model of ADMA and hsCRP caused FMD impairment when the positivity of β2GPIIgG was added. ADMA may be used as a simple and low-cost tool for verifying the presence of endothelial dysfunction in APS patients. According to the results of the study, we could presume that hsCRP, together with aPL, has a preparatory effect on the endothelium in causing endothelial dysfunction.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - Asymmetric Dimethylarginine Is a Marker of Endothelial Dysfunction in Thrombotic Antiphospholipid Syndrome Patients
VL  - 23
IS  - 20
DO  - 10.3390/ijms232012309
ER  - 

@article{
author = "Stanisavljević, Nataša and Stojanovich, Ljudmila and Đoković, Aleksandra and Todić, Brankica and Dopsaj, Violeta and Saponjski, Jovica and Saponjski, Dušan and Marković, Olivera and Belizna, Cristina and Zdravković, Marija and Marisavljević, Dragomir",
year = "2022",
abstract = "Objective: The potential contribution of asymmetric dimethylarginine (ADMA) and high-sensitivity C reactive protein (hsCRP) to endothelial dysfunction in APS patients has not been studied in detail, until now. The study involved 105 APS patients (59 diagnosed with primary APS (PAPS) and 46 APS associated with systemic lupus erythematosus (SAPS)) who were compared to 40 controls. Endothelial dysfunction was assessed by measurement of flow-mediated dilatation (FMD) and glyceryl trinitrate dilatation (NMD) of the brachial artery. ADMA (micromol/L) was analyzed by ELISA. Results: FMD in patients with APS was significantly lower than that of the controls (p < 0.001), with no difference between the PAPS and the SAPS groups. ADMA and hsCRP concentrations were significantly higher in the patient cohort than in the control group (p < 0.001, p = 0.006, respectively), as was the case with the SAPS group as compared to the PAPS group (p < 0.001, p = 0.022, respectively). FMD impairment correlated to ADMA (ρ 0.472, p < 0.001) and to hsCRP (ρ 0.181, p = 0.033). In the regression model, the ADMA concentration confirmed the strength of its association (B 0.518, SE 0.183, Wald 8.041, p = 0.005, Exp(B) 1.679, 95% CI 1.174–2.402) to FMD impairment. The synergistic probability model of ADMA and hsCRP caused FMD impairment when the positivity of β2GPIIgG was added. ADMA may be used as a simple and low-cost tool for verifying the presence of endothelial dysfunction in APS patients. According to the results of the study, we could presume that hsCRP, together with aPL, has a preparatory effect on the endothelium in causing endothelial dysfunction.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "Asymmetric Dimethylarginine Is a Marker of Endothelial Dysfunction in Thrombotic Antiphospholipid Syndrome Patients",
volume = "23",
number = "20",
doi = "10.3390/ijms232012309"
}

Stanisavljević, N., Stojanovich, L., Đoković, A., Todić, B., Dopsaj, V., Saponjski, J., Saponjski, D., Marković, O., Belizna, C., Zdravković, M.,& Marisavljević, D.. (2022). Asymmetric Dimethylarginine Is a Marker of Endothelial Dysfunction in Thrombotic Antiphospholipid Syndrome Patients. in International Journal of Molecular Sciences
MDPI., 23(20).
https://doi.org/10.3390/ijms232012309

Stanisavljević N, Stojanovich L, Đoković A, Todić B, Dopsaj V, Saponjski J, Saponjski D, Marković O, Belizna C, Zdravković M, Marisavljević D. Asymmetric Dimethylarginine Is a Marker of Endothelial Dysfunction in Thrombotic Antiphospholipid Syndrome Patients. in International Journal of Molecular Sciences. 2022;23(20).
doi:10.3390/ijms232012309 .

Stanisavljević, Nataša, Stojanovich, Ljudmila, Đoković, Aleksandra, Todić, Brankica, Dopsaj, Violeta, Saponjski, Jovica, Saponjski, Dušan, Marković, Olivera, Belizna, Cristina, Zdravković, Marija, Marisavljević, Dragomir, "Asymmetric Dimethylarginine Is a Marker of Endothelial Dysfunction in Thrombotic Antiphospholipid Syndrome Patients" in International Journal of Molecular Sciences, 23, no. 20 (2022),
https://doi.org/10.3390/ijms232012309 . .

TY  - JOUR
AU  - Dopsaj, Violeta
AU  - Topić, Aleksandra
AU  - Savković, Miljan
AU  - Milinković, Neda
AU  - Novaković, Ivana
AU  - Cujić, Danica
AU  - Simić-Ogrizović, Sanja
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3366
AB  - Background. Influence of TMPRSS6 A736V and HFE (C282Y and H63D) polymorphisms on serum hepcidin-25 levels and iron status parameters in end-stage renal disease (ESRD) patients stratified according to gender has not been previously investigated. In addition, we aimed to evaluate the diagnostic accuracy of the parameters to separate iron-deficiency anemia (IDA) from anemia of chronic disease. Materials and Methods. Iron status parameters and genetic analysis were performed in 126 ESRD patients and in 31 IDA patients as the control group. Results. ESRD patients had significantly higher ferritin and hepcidin-25 ( lt 0.001) relative to IDA patients. Cut-off values with the best diagnostic accuracy were found for hepcidin 9.32ng/mL, ferritin 48.2g/L, transferrin saturation 16.8%, and MCV 81fL. Interaction between gender and HFE haplotypes for the hepcidin-25 and ferritin levels in ESRD patients (p=0.005, partial eta squared=0.09; p=0.027, partial eta squared=0.06, respectively) was found. Serum transferrin was influenced by the combined effect of gender and TMPRSS6 A736V polymorphism in ESRD patients (p=0.002, partial eta squared=0.07). Conclusion. Our findings could contribute to the further investigation of mechanisms involved in the pathophysiology and important gender-related involvement of the TMPRSS6 and HFE polymorphisms on anemia in ESRD patients.
PB  - Hindawi Ltd, London
T2  - Disease Markers
T1  - Associations of Common Variants in HFE and TMPRSS6 Genes with Hepcidin-25 and Iron Status Parameters in Patients with End-Stage Renal Disease
DO  - 10.1155/2019/4864370
ER  - 

@article{
author = "Dopsaj, Violeta and Topić, Aleksandra and Savković, Miljan and Milinković, Neda and Novaković, Ivana and Cujić, Danica and Simić-Ogrizović, Sanja",
year = "2019",
abstract = "Background. Influence of TMPRSS6 A736V and HFE (C282Y and H63D) polymorphisms on serum hepcidin-25 levels and iron status parameters in end-stage renal disease (ESRD) patients stratified according to gender has not been previously investigated. In addition, we aimed to evaluate the diagnostic accuracy of the parameters to separate iron-deficiency anemia (IDA) from anemia of chronic disease. Materials and Methods. Iron status parameters and genetic analysis were performed in 126 ESRD patients and in 31 IDA patients as the control group. Results. ESRD patients had significantly higher ferritin and hepcidin-25 ( lt 0.001) relative to IDA patients. Cut-off values with the best diagnostic accuracy were found for hepcidin 9.32ng/mL, ferritin 48.2g/L, transferrin saturation 16.8%, and MCV 81fL. Interaction between gender and HFE haplotypes for the hepcidin-25 and ferritin levels in ESRD patients (p=0.005, partial eta squared=0.09; p=0.027, partial eta squared=0.06, respectively) was found. Serum transferrin was influenced by the combined effect of gender and TMPRSS6 A736V polymorphism in ESRD patients (p=0.002, partial eta squared=0.07). Conclusion. Our findings could contribute to the further investigation of mechanisms involved in the pathophysiology and important gender-related involvement of the TMPRSS6 and HFE polymorphisms on anemia in ESRD patients.",
publisher = "Hindawi Ltd, London",
journal = "Disease Markers",
title = "Associations of Common Variants in HFE and TMPRSS6 Genes with Hepcidin-25 and Iron Status Parameters in Patients with End-Stage Renal Disease",
doi = "10.1155/2019/4864370"
}

Dopsaj, V., Topić, A., Savković, M., Milinković, N., Novaković, I., Cujić, D.,& Simić-Ogrizović, S.. (2019). Associations of Common Variants in HFE and TMPRSS6 Genes with Hepcidin-25 and Iron Status Parameters in Patients with End-Stage Renal Disease. in Disease Markers
Hindawi Ltd, London..
https://doi.org/10.1155/2019/4864370

Dopsaj V, Topić A, Savković M, Milinković N, Novaković I, Cujić D, Simić-Ogrizović S. Associations of Common Variants in HFE and TMPRSS6 Genes with Hepcidin-25 and Iron Status Parameters in Patients with End-Stage Renal Disease. in Disease Markers. 2019;.
doi:10.1155/2019/4864370 .

Dopsaj, Violeta, Topić, Aleksandra, Savković, Miljan, Milinković, Neda, Novaković, Ivana, Cujić, Danica, Simić-Ogrizović, Sanja, "Associations of Common Variants in HFE and TMPRSS6 Genes with Hepcidin-25 and Iron Status Parameters in Patients with End-Stage Renal Disease" in Disease Markers (2019),
https://doi.org/10.1155/2019/4864370 . .

TY  - JOUR
AU  - Totosković, Dragana
AU  - Dopsaj, Violeta
AU  - Martinović, Jelena
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2803
AB  - The purpose of the present study was to investigate neutrophil and monocyte cell population data as novel markers of low cobalamin/folate concentrations and influence of renal function on their usefulness. The study included 284 patients older than 60 years or with dyspepsia symptoms with mean corpuscular volume 80-100 fL and C-reactive protein  lt = 50 mg/l. Subjects were divided according to renal function and further classified based on cobalamin and folate levels. Neutrophil and monocyte volume (NeV, MoV), conductivity (NeC, MoC), light scatter (NeS, MoS) and standard deviations (NeV-SD, MoV-SD, NeCSD, MoC-SD, NeS-SD, MoS-SD), obtained by Coulter LH750 (R) Hematology Analyzer (Beckman Coulter, USA), were evaluated along with white blood cell count, hemoglobin, hematocrit, red cell distribution width and homocysteine relative to renal function and cobalamin/folate status. Neutrophil conductivity standard deviation (NeC-SD) had the largest magnitude of the difference between patients with low and normal vitamin levels, was the strongest predictor of low cobalamin/folate concentrations and had the largest area under the curve in detection of vitamin deficiency. Patients with different renal function status and the same cobalamin/folate status did not differ in NeC-SD. In this selected group of patients, NeC-SD was marker of low cobalamin and folate levels regardless of the renal function.
PB  - Univ South Bohemia, Ceska Budejovice
T2  - Journal of Applied Biomedicine
T1  - Evaluation of cell population data as potential markers of cobalamin and folate deficiency in populations at risk with regard to renal function
VL  - 15
IS  - 3
SP  - 204
EP  - 209
DO  - 10.1016/j.jab.2017.02.002
ER  - 

@article{
author = "Totosković, Dragana and Dopsaj, Violeta and Martinović, Jelena",
year = "2017",
abstract = "The purpose of the present study was to investigate neutrophil and monocyte cell population data as novel markers of low cobalamin/folate concentrations and influence of renal function on their usefulness. The study included 284 patients older than 60 years or with dyspepsia symptoms with mean corpuscular volume 80-100 fL and C-reactive protein  lt = 50 mg/l. Subjects were divided according to renal function and further classified based on cobalamin and folate levels. Neutrophil and monocyte volume (NeV, MoV), conductivity (NeC, MoC), light scatter (NeS, MoS) and standard deviations (NeV-SD, MoV-SD, NeCSD, MoC-SD, NeS-SD, MoS-SD), obtained by Coulter LH750 (R) Hematology Analyzer (Beckman Coulter, USA), were evaluated along with white blood cell count, hemoglobin, hematocrit, red cell distribution width and homocysteine relative to renal function and cobalamin/folate status. Neutrophil conductivity standard deviation (NeC-SD) had the largest magnitude of the difference between patients with low and normal vitamin levels, was the strongest predictor of low cobalamin/folate concentrations and had the largest area under the curve in detection of vitamin deficiency. Patients with different renal function status and the same cobalamin/folate status did not differ in NeC-SD. In this selected group of patients, NeC-SD was marker of low cobalamin and folate levels regardless of the renal function.",
publisher = "Univ South Bohemia, Ceska Budejovice",
journal = "Journal of Applied Biomedicine",
title = "Evaluation of cell population data as potential markers of cobalamin and folate deficiency in populations at risk with regard to renal function",
volume = "15",
number = "3",
pages = "204-209",
doi = "10.1016/j.jab.2017.02.002"
}

Totosković, D., Dopsaj, V.,& Martinović, J.. (2017). Evaluation of cell population data as potential markers of cobalamin and folate deficiency in populations at risk with regard to renal function. in Journal of Applied Biomedicine
Univ South Bohemia, Ceska Budejovice., 15(3), 204-209.
https://doi.org/10.1016/j.jab.2017.02.002

Totosković D, Dopsaj V, Martinović J. Evaluation of cell population data as potential markers of cobalamin and folate deficiency in populations at risk with regard to renal function. in Journal of Applied Biomedicine. 2017;15(3):204-209.
doi:10.1016/j.jab.2017.02.002 .

Totosković, Dragana, Dopsaj, Violeta, Martinović, Jelena, "Evaluation of cell population data as potential markers of cobalamin and folate deficiency in populations at risk with regard to renal function" in Journal of Applied Biomedicine, 15, no. 3 (2017):204-209,
https://doi.org/10.1016/j.jab.2017.02.002 . .

TY  - CONF
AU  - Stojanović, Ljudmila
AU  - Stanisavljević, Nataša
AU  - Marisavljević, D.
AU  - Dopsaj, Violeta
AU  - Todić, B.
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2648
PB  - BMJ PUBLISHING GROUP, LONDON
C3  - Annals of the Rheumatic Diseases
T1  - The oxidative stress markers as risk factor for endothelial dysfunction in primary and secondary antiphospholipid syndrome
VL  - 75
IS  - Supplement 2
SP  - 284
EP  - 284
DO  - 10.1136/annrheumdis-2016-eular.1782
ER  - 

@conference{
author = "Stojanović, Ljudmila and Stanisavljević, Nataša and Marisavljević, D. and Dopsaj, Violeta and Todić, B.",
year = "2016",
publisher = "BMJ PUBLISHING GROUP, LONDON",
journal = "Annals of the Rheumatic Diseases",
title = "The oxidative stress markers as risk factor for endothelial dysfunction in primary and secondary antiphospholipid syndrome",
volume = "75",
number = "Supplement 2",
pages = "284-284",
doi = "10.1136/annrheumdis-2016-eular.1782"
}

Stojanović, L., Stanisavljević, N., Marisavljević, D., Dopsaj, V.,& Todić, B.. (2016). The oxidative stress markers as risk factor for endothelial dysfunction in primary and secondary antiphospholipid syndrome. in Annals of the Rheumatic Diseases
BMJ PUBLISHING GROUP, LONDON., 75(Supplement 2), 284-284.
https://doi.org/10.1136/annrheumdis-2016-eular.1782

Stojanović L, Stanisavljević N, Marisavljević D, Dopsaj V, Todić B. The oxidative stress markers as risk factor for endothelial dysfunction in primary and secondary antiphospholipid syndrome. in Annals of the Rheumatic Diseases. 2016;75(Supplement 2):284-284.
doi:10.1136/annrheumdis-2016-eular.1782 .

Stojanović, Ljudmila, Stanisavljević, Nataša, Marisavljević, D., Dopsaj, Violeta, Todić, B., "The oxidative stress markers as risk factor for endothelial dysfunction in primary and secondary antiphospholipid syndrome" in Annals of the Rheumatic Diseases, 75, no. Supplement 2 (2016):284-284,
https://doi.org/10.1136/annrheumdis-2016-eular.1782 . .

TY  - JOUR
AU  - Becarević, Mirjana
AU  - Stojanović, Ljudmila
AU  - Ignjatović, Svetlana
AU  - Dopsaj, Violeta
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2590
AB  - We evaluated the importance of anti-annexin A5 antibodies (aanxA5 Abs) for clinical (thrombosis and/or recurrent pregnancy loss) and serologic (presence of antiphospholipid antibodies: lupus anticoagulant (LA), anticardiolipin (aCL), and anti-beta 2 glycoprotein I (a beta 2GPI) antibodies) features of patients with primary antiphospholipid syndrome (PAPS). Our study included 70 patients with PAPS according to the international consensus criteria for APS. The mean age of the analyzed patients was 45.97 +/- 12.72. The disease duration above 5 years was present in 31/70 of patients. Concentrations of analyzed antibodies were measured by ELISA. Cutoff values were set in accordance to the manufacturers' recommendations. History of recurrent pregnancy loss was associated with double positivity for aanxA5 IgM and LA (chi (2) = 4.000, P = 0.046) and triple positivity for aanxA5 IgM + LA + a beta 2GPI IgM (chi (2) = 4.168, P = 0.041). Venous thromboses were associated with triple positivity for aanxA5 IgM + aCLIgG + a beta 2GPI IgM (chi (2) = 3.965, P = 0.046). The IgG isotype of aanxA5 Abs was in positive correlation with aCL Abs of the IgG (r = 0.310, P = 0.009) and IgM (r = 0.254, P = 0.034) isotype. The presence of the clinical manifestations of PAPS is increasing with the number of positive conventional aPL and the IgM aanxA5 Abs tests. This new combination of Abs is beneficial even when the number of patients with positivity for aanxA5 Abs is low. This is important in further detection of patients prone to recurrence of thrombotic episodes.
PB  - Springer London Ltd, London
T2  - Clinical Rheumatology
T1  - The IgM isotype of anti-annexin A5 antibodies and multiple positivity of conventional antiphospholipid antibodies: increasing the number of clinical manifestations of primary antiphospholipid syndrome
VL  - 35
IS  - 5
SP  - 1361
EP  - 1365
DO  - 10.1007/s10067-016-3230-0
ER  - 

@article{
author = "Becarević, Mirjana and Stojanović, Ljudmila and Ignjatović, Svetlana and Dopsaj, Violeta",
year = "2016",
abstract = "We evaluated the importance of anti-annexin A5 antibodies (aanxA5 Abs) for clinical (thrombosis and/or recurrent pregnancy loss) and serologic (presence of antiphospholipid antibodies: lupus anticoagulant (LA), anticardiolipin (aCL), and anti-beta 2 glycoprotein I (a beta 2GPI) antibodies) features of patients with primary antiphospholipid syndrome (PAPS). Our study included 70 patients with PAPS according to the international consensus criteria for APS. The mean age of the analyzed patients was 45.97 +/- 12.72. The disease duration above 5 years was present in 31/70 of patients. Concentrations of analyzed antibodies were measured by ELISA. Cutoff values were set in accordance to the manufacturers' recommendations. History of recurrent pregnancy loss was associated with double positivity for aanxA5 IgM and LA (chi (2) = 4.000, P = 0.046) and triple positivity for aanxA5 IgM + LA + a beta 2GPI IgM (chi (2) = 4.168, P = 0.041). Venous thromboses were associated with triple positivity for aanxA5 IgM + aCLIgG + a beta 2GPI IgM (chi (2) = 3.965, P = 0.046). The IgG isotype of aanxA5 Abs was in positive correlation with aCL Abs of the IgG (r = 0.310, P = 0.009) and IgM (r = 0.254, P = 0.034) isotype. The presence of the clinical manifestations of PAPS is increasing with the number of positive conventional aPL and the IgM aanxA5 Abs tests. This new combination of Abs is beneficial even when the number of patients with positivity for aanxA5 Abs is low. This is important in further detection of patients prone to recurrence of thrombotic episodes.",
publisher = "Springer London Ltd, London",
journal = "Clinical Rheumatology",
title = "The IgM isotype of anti-annexin A5 antibodies and multiple positivity of conventional antiphospholipid antibodies: increasing the number of clinical manifestations of primary antiphospholipid syndrome",
volume = "35",
number = "5",
pages = "1361-1365",
doi = "10.1007/s10067-016-3230-0"
}

Becarević, M., Stojanović, L., Ignjatović, S.,& Dopsaj, V.. (2016). The IgM isotype of anti-annexin A5 antibodies and multiple positivity of conventional antiphospholipid antibodies: increasing the number of clinical manifestations of primary antiphospholipid syndrome. in Clinical Rheumatology
Springer London Ltd, London., 35(5), 1361-1365.
https://doi.org/10.1007/s10067-016-3230-0

Becarević M, Stojanović L, Ignjatović S, Dopsaj V. The IgM isotype of anti-annexin A5 antibodies and multiple positivity of conventional antiphospholipid antibodies: increasing the number of clinical manifestations of primary antiphospholipid syndrome. in Clinical Rheumatology. 2016;35(5):1361-1365.
doi:10.1007/s10067-016-3230-0 .

Becarević, Mirjana, Stojanović, Ljudmila, Ignjatović, Svetlana, Dopsaj, Violeta, "The IgM isotype of anti-annexin A5 antibodies and multiple positivity of conventional antiphospholipid antibodies: increasing the number of clinical manifestations of primary antiphospholipid syndrome" in Clinical Rheumatology, 35, no. 5 (2016):1361-1365,
https://doi.org/10.1007/s10067-016-3230-0 . .

TY  - JOUR
AU  - Totosković, Dragana
AU  - Dopsaj, Violeta
AU  - Martinović, Jelena
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2589
AB  - IntroductionThis study aimed to investigate the utility of methylmalonic acid (MMA) and neutrophil Cell Population Data, available on the Beckman Coulter LH750 Analyser (Miami, FL, USA), in laboratory assessment of cobalamin status in patients at risk of cobalamin deficiency, without macrocytosis and inflammation. MethodsThe study group included 189 patients. Neutrophil Cell Population Data along with vitamin B12 and homocysteine were assessed in regard to MMA tertile groups. ResultsStatistically significant differences were between lower and upper MMA tertile in serum B12 (P ? 0.001), homocysteine (P = 0.001) and neutrophil morphometric index, NeS-DW (P = 0.029). Also, serum B12 concentrations were significantly different between lower and middle MMA tertile, P = 0.005. Receiver operating characteristic analysis of NeS-DW ability to detect MMA?367 nmol/L revealed a significant area under the curve AUC = 0.761 P ? 0.001 95% CI 0.693-0.830. Optimal cut-off value was NeS-DW?3.51% with sensitivity of 74.19% and specificity of 68.87%. ConclusionIn patients at risk of cobalamin deficiency and normal MCV, classification according to MMA revealed cobalamin status differences. Neutrophil morphometric index may be an indicator of early changes in neutrophil nucleus morphology caused by impaired cobalamin status.
PB  - Wiley-Blackwell, Hoboken
T2  - International Journal of Laboratory Hematology
T1  - Methylmalonic acid and neutrophil morphometric index in laboratory diagnosis of cobalamin deficiency without macrocytosis
VL  - 38
IS  - 3
SP  - 265
EP  - 272
DO  - 10.1111/ijlh.12479
ER  - 

@article{
author = "Totosković, Dragana and Dopsaj, Violeta and Martinović, Jelena",
year = "2016",
abstract = "IntroductionThis study aimed to investigate the utility of methylmalonic acid (MMA) and neutrophil Cell Population Data, available on the Beckman Coulter LH750 Analyser (Miami, FL, USA), in laboratory assessment of cobalamin status in patients at risk of cobalamin deficiency, without macrocytosis and inflammation. MethodsThe study group included 189 patients. Neutrophil Cell Population Data along with vitamin B12 and homocysteine were assessed in regard to MMA tertile groups. ResultsStatistically significant differences were between lower and upper MMA tertile in serum B12 (P ? 0.001), homocysteine (P = 0.001) and neutrophil morphometric index, NeS-DW (P = 0.029). Also, serum B12 concentrations were significantly different between lower and middle MMA tertile, P = 0.005. Receiver operating characteristic analysis of NeS-DW ability to detect MMA?367 nmol/L revealed a significant area under the curve AUC = 0.761 P ? 0.001 95% CI 0.693-0.830. Optimal cut-off value was NeS-DW?3.51% with sensitivity of 74.19% and specificity of 68.87%. ConclusionIn patients at risk of cobalamin deficiency and normal MCV, classification according to MMA revealed cobalamin status differences. Neutrophil morphometric index may be an indicator of early changes in neutrophil nucleus morphology caused by impaired cobalamin status.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "International Journal of Laboratory Hematology",
title = "Methylmalonic acid and neutrophil morphometric index in laboratory diagnosis of cobalamin deficiency without macrocytosis",
volume = "38",
number = "3",
pages = "265-272",
doi = "10.1111/ijlh.12479"
}

Totosković, D., Dopsaj, V.,& Martinović, J.. (2016). Methylmalonic acid and neutrophil morphometric index in laboratory diagnosis of cobalamin deficiency without macrocytosis. in International Journal of Laboratory Hematology
Wiley-Blackwell, Hoboken., 38(3), 265-272.
https://doi.org/10.1111/ijlh.12479

Totosković D, Dopsaj V, Martinović J. Methylmalonic acid and neutrophil morphometric index in laboratory diagnosis of cobalamin deficiency without macrocytosis. in International Journal of Laboratory Hematology. 2016;38(3):265-272.
doi:10.1111/ijlh.12479 .

Totosković, Dragana, Dopsaj, Violeta, Martinović, Jelena, "Methylmalonic acid and neutrophil morphometric index in laboratory diagnosis of cobalamin deficiency without macrocytosis" in International Journal of Laboratory Hematology, 38, no. 3 (2016):265-272,
https://doi.org/10.1111/ijlh.12479 . .

TY  - JOUR
AU  - Dopsaj, Violeta
AU  - Martinović, Jelena
AU  - Dopsaj, Milivoj
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2171
AB  - Introduction: The aim of this study was to establish the sensitivity and specificity of the microcytic anemia factor (Maf((R))), which is generated by the Coulter LH 750 analyzer in the evaluation of iron depletion and iron-deficient erythropoiesis in athletes. Methods: A total of 142 athletes were divided into three groups: with iron depletion, with iron-deficient erythropoiesis, and controls. The following parameters were measured: RBCs (red blood cells), Hb (hemoglobin), Hct (hematocrit), MCV (mean cellular volume), MCH (mean cell hemoglobin), MCHC (mean cell hemoglobin corpuscular), RDW (red cell distribution width), Maf((R)), Reticulocytes, Ferritin, sTfR (soluble transferrin receptor), Transferrin, Haptoglobin, IL- 6 (Interleukin-6), hs-CRP (high-sensitivity C-reactive protein). Results: The best Maf((R)) value to exclude iron depletion in athletes was 130.3, showing a sensitivity of 72.6% and a specificity of 57.3%. The AUC was 0.690 (CI 95% 0.607-0.765, P lt 0.001). ROC curve analysis for Maf((R)) in the diagnosis of iron-deficient erythropoiesis indicates sensitivity of 61.5%, and specificity of 93.0%, with AUC=0.826 (CI 95% 0.754-0.885, P lt 0.001) and on cutoff value 114. Conclusions: This study shows that Maf((R)), generated by the Coulter LH 700 Series hematology analyzers, performs very well in discriminating healthy athletes and those with different stages of iron deficiency. Also, in cost/benefit terms, monitoring of Maf((R)) is justified as a low cost, effective screening parameter for determining iron status in athletes.
PB  - Wiley-Blackwell, Hoboken
T2  - International Journal of Laboratory Hematology
T1  - Early detection of iron deficiency in elite athletes: could microcytic anemia factor (Maf) be useful?
VL  - 36
IS  - 1
SP  - 37
EP  - 44
DO  - 10.1111/ijlh.12115
ER  - 

@article{
author = "Dopsaj, Violeta and Martinović, Jelena and Dopsaj, Milivoj",
year = "2014",
abstract = "Introduction: The aim of this study was to establish the sensitivity and specificity of the microcytic anemia factor (Maf((R))), which is generated by the Coulter LH 750 analyzer in the evaluation of iron depletion and iron-deficient erythropoiesis in athletes. Methods: A total of 142 athletes were divided into three groups: with iron depletion, with iron-deficient erythropoiesis, and controls. The following parameters were measured: RBCs (red blood cells), Hb (hemoglobin), Hct (hematocrit), MCV (mean cellular volume), MCH (mean cell hemoglobin), MCHC (mean cell hemoglobin corpuscular), RDW (red cell distribution width), Maf((R)), Reticulocytes, Ferritin, sTfR (soluble transferrin receptor), Transferrin, Haptoglobin, IL- 6 (Interleukin-6), hs-CRP (high-sensitivity C-reactive protein). Results: The best Maf((R)) value to exclude iron depletion in athletes was 130.3, showing a sensitivity of 72.6% and a specificity of 57.3%. The AUC was 0.690 (CI 95% 0.607-0.765, P lt 0.001). ROC curve analysis for Maf((R)) in the diagnosis of iron-deficient erythropoiesis indicates sensitivity of 61.5%, and specificity of 93.0%, with AUC=0.826 (CI 95% 0.754-0.885, P lt 0.001) and on cutoff value 114. Conclusions: This study shows that Maf((R)), generated by the Coulter LH 700 Series hematology analyzers, performs very well in discriminating healthy athletes and those with different stages of iron deficiency. Also, in cost/benefit terms, monitoring of Maf((R)) is justified as a low cost, effective screening parameter for determining iron status in athletes.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "International Journal of Laboratory Hematology",
title = "Early detection of iron deficiency in elite athletes: could microcytic anemia factor (Maf) be useful?",
volume = "36",
number = "1",
pages = "37-44",
doi = "10.1111/ijlh.12115"
}

Dopsaj, V., Martinović, J.,& Dopsaj, M.. (2014). Early detection of iron deficiency in elite athletes: could microcytic anemia factor (Maf) be useful?. in International Journal of Laboratory Hematology
Wiley-Blackwell, Hoboken., 36(1), 37-44.
https://doi.org/10.1111/ijlh.12115

Dopsaj V, Martinović J, Dopsaj M. Early detection of iron deficiency in elite athletes: could microcytic anemia factor (Maf) be useful?. in International Journal of Laboratory Hematology. 2014;36(1):37-44.
doi:10.1111/ijlh.12115 .

Dopsaj, Violeta, Martinović, Jelena, Dopsaj, Milivoj, "Early detection of iron deficiency in elite athletes: could microcytic anemia factor (Maf) be useful?" in International Journal of Laboratory Hematology, 36, no. 1 (2014):37-44,
https://doi.org/10.1111/ijlh.12115 . .

TY  - JOUR
AU  - Đunić, Irena
AU  - Dopsaj, Violeta
AU  - Miljić, Predrag
AU  - Savić, Nebojša
AU  - Milić, Nataša
AU  - Mitrović, Mirjana
AU  - Suvajdžić-Vuković, Nada
AU  - Tomin, Dragica
AU  - Elezović, Ivo
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2082
AB  - Background: The goal of this study was to compare the validity of two laboratory assays, rotation thromboelastometry (ROTEM) and endogenous thrombin potential (ETP), in monitoring and evaluating different prophylactic treatment regimens in patients with severe haemophilia. Methods: Twenty adult patients with severe haemophilia were divided into three groups according to treatment regimen with concentrate of factor (F) VIII/IX: full-dose prophylaxis (5 patients), intermediate-dose prophylaxis (5 patients), and on demand treatment (10 patients). Results: The ROTEM for the group treated with full-dose prophylaxis was significantly lower than ROTEM for the group treated with intermediate-dose prophylaxis (p = 0.025). Among the patients given full-dose prophylaxis, 40% (2 patients) had prolonged ROTEM after 3 months of treatment, while among those given intermediate-dose prophylaxis all patients (100%, 5 patients) had prolonged ROTEM (p = 0.038). The ETP was significantly improved after 3 months of full-dose in comparison with intermediate-dose prophylaxis (p = 0.042). Conclusions: ROTEM and ETP are useful laboratory assays for monitoring efficacy of different prophylaxis regimens with concentrate of FVIII/IX in patients with severe haemophilia, helping in making decisions regarding optimal dose-regimen prophylaxis.
PB  - Clin Lab Publ, Heidelberg
T2  - Clinical Laboratory
T1  - Comparison of Two Laboratory Assays in Monitoring the Efficacy of Different Prophylaxis Regimens for Severe Haemophilia
VL  - 60
IS  - 8
SP  - 1325
EP  - 1331
DO  - 10.7754/Clin.Lab.2013.130817
ER  - 

@article{
author = "Đunić, Irena and Dopsaj, Violeta and Miljić, Predrag and Savić, Nebojša and Milić, Nataša and Mitrović, Mirjana and Suvajdžić-Vuković, Nada and Tomin, Dragica and Elezović, Ivo",
year = "2014",
abstract = "Background: The goal of this study was to compare the validity of two laboratory assays, rotation thromboelastometry (ROTEM) and endogenous thrombin potential (ETP), in monitoring and evaluating different prophylactic treatment regimens in patients with severe haemophilia. Methods: Twenty adult patients with severe haemophilia were divided into three groups according to treatment regimen with concentrate of factor (F) VIII/IX: full-dose prophylaxis (5 patients), intermediate-dose prophylaxis (5 patients), and on demand treatment (10 patients). Results: The ROTEM for the group treated with full-dose prophylaxis was significantly lower than ROTEM for the group treated with intermediate-dose prophylaxis (p = 0.025). Among the patients given full-dose prophylaxis, 40% (2 patients) had prolonged ROTEM after 3 months of treatment, while among those given intermediate-dose prophylaxis all patients (100%, 5 patients) had prolonged ROTEM (p = 0.038). The ETP was significantly improved after 3 months of full-dose in comparison with intermediate-dose prophylaxis (p = 0.042). Conclusions: ROTEM and ETP are useful laboratory assays for monitoring efficacy of different prophylaxis regimens with concentrate of FVIII/IX in patients with severe haemophilia, helping in making decisions regarding optimal dose-regimen prophylaxis.",
publisher = "Clin Lab Publ, Heidelberg",
journal = "Clinical Laboratory",
title = "Comparison of Two Laboratory Assays in Monitoring the Efficacy of Different Prophylaxis Regimens for Severe Haemophilia",
volume = "60",
number = "8",
pages = "1325-1331",
doi = "10.7754/Clin.Lab.2013.130817"
}

Đunić, I., Dopsaj, V., Miljić, P., Savić, N., Milić, N., Mitrović, M., Suvajdžić-Vuković, N., Tomin, D.,& Elezović, I.. (2014). Comparison of Two Laboratory Assays in Monitoring the Efficacy of Different Prophylaxis Regimens for Severe Haemophilia. in Clinical Laboratory
Clin Lab Publ, Heidelberg., 60(8), 1325-1331.
https://doi.org/10.7754/Clin.Lab.2013.130817

Đunić I, Dopsaj V, Miljić P, Savić N, Milić N, Mitrović M, Suvajdžić-Vuković N, Tomin D, Elezović I. Comparison of Two Laboratory Assays in Monitoring the Efficacy of Different Prophylaxis Regimens for Severe Haemophilia. in Clinical Laboratory. 2014;60(8):1325-1331.
doi:10.7754/Clin.Lab.2013.130817 .

Đunić, Irena, Dopsaj, Violeta, Miljić, Predrag, Savić, Nebojša, Milić, Nataša, Mitrović, Mirjana, Suvajdžić-Vuković, Nada, Tomin, Dragica, Elezović, Ivo, "Comparison of Two Laboratory Assays in Monitoring the Efficacy of Different Prophylaxis Regimens for Severe Haemophilia" in Clinical Laboratory, 60, no. 8 (2014):1325-1331,
https://doi.org/10.7754/Clin.Lab.2013.130817 . .

TY  - JOUR
AU  - Dopsaj, Violeta
AU  - Martinović, Jelena
AU  - Dopsaj, Milivoj
AU  - Kasum, Goran
AU  - Kotur-Stevuljević, Jelena
AU  - Koropanovski, Nenad
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1900
AB  - The aim of this study was to profile hematological, oxidative stress, and immunological parameters in male athletes who practiced combat sports and to determine whether the type of combat sport influenced the measured parameters. Eighteen karate professionals, 15 wrestlers, and 14 kickboxers participated in the study. Hematological, iron-related, oxidative stress, and immunological parameters were measured at the beginning of a precompetitive period. The general linear model showed significant differences between the karate professionals, wrestlers, and kickboxers with respect to their hematological and iron status parameters (Wilks' Lambda = 0.270, F = 2.186, p  lt  0.05) and oxidative stress status (Wilks' Lambda = 0.529, F = 1.940, p  lt  0.05). The immature reticulocyte fraction was significantly higher in wrestlers (0.30 +/- 0.03) compared with kickboxers (0.24 +/- 0.04; p  lt  0.05) and karate professionals (0.26 +/- 0.04; p  lt  0.05). Low hemoglobin density was significantly lower in wrestlers and kickboxers (p  lt  0.05) compared with karate professionals (karate: 3.51 +/- 1.19, wrestlers: 1.95 +/- 1.10, and kickboxers: 1.77 +/- 0.76). Significant differences were observed between the karate professionals and wrestlers with respect to their pro-oxidant-antioxidant balance (437 +/- 103 vs. 323 +/- 148, p  lt  0.05) and superoxide-dismutase activity (SOD) (73 +/- 37 vs. 103 +/- 30, p  lt  0.05). All the measured parameters (with the exception of SOD activity) fell within their physiological ranges, indicating that the study participants represented a young and healthy male population. Hematological parameters differed between kickboxers and karate professionals. The low pro-oxidant-antioxidant balance and high SOD activity in wrestlers could be associated with the long-term impact of wrestling as a type of strenuous exercise.
PB  - Lippincott Williams & Wilkins, Philadelphia
T2  - Journal of Strength and Conditioning Research
T1  - Hematological, oxidative stress, and immune status profiling in elite combat sport athletes
VL  - 27
IS  - 12
SP  - 3506
EP  - 3514
DO  - 10.1519/JSC.0b013e31828ddeea
ER  - 

@article{
author = "Dopsaj, Violeta and Martinović, Jelena and Dopsaj, Milivoj and Kasum, Goran and Kotur-Stevuljević, Jelena and Koropanovski, Nenad",
year = "2013",
abstract = "The aim of this study was to profile hematological, oxidative stress, and immunological parameters in male athletes who practiced combat sports and to determine whether the type of combat sport influenced the measured parameters. Eighteen karate professionals, 15 wrestlers, and 14 kickboxers participated in the study. Hematological, iron-related, oxidative stress, and immunological parameters were measured at the beginning of a precompetitive period. The general linear model showed significant differences between the karate professionals, wrestlers, and kickboxers with respect to their hematological and iron status parameters (Wilks' Lambda = 0.270, F = 2.186, p  lt  0.05) and oxidative stress status (Wilks' Lambda = 0.529, F = 1.940, p  lt  0.05). The immature reticulocyte fraction was significantly higher in wrestlers (0.30 +/- 0.03) compared with kickboxers (0.24 +/- 0.04; p  lt  0.05) and karate professionals (0.26 +/- 0.04; p  lt  0.05). Low hemoglobin density was significantly lower in wrestlers and kickboxers (p  lt  0.05) compared with karate professionals (karate: 3.51 +/- 1.19, wrestlers: 1.95 +/- 1.10, and kickboxers: 1.77 +/- 0.76). Significant differences were observed between the karate professionals and wrestlers with respect to their pro-oxidant-antioxidant balance (437 +/- 103 vs. 323 +/- 148, p  lt  0.05) and superoxide-dismutase activity (SOD) (73 +/- 37 vs. 103 +/- 30, p  lt  0.05). All the measured parameters (with the exception of SOD activity) fell within their physiological ranges, indicating that the study participants represented a young and healthy male population. Hematological parameters differed between kickboxers and karate professionals. The low pro-oxidant-antioxidant balance and high SOD activity in wrestlers could be associated with the long-term impact of wrestling as a type of strenuous exercise.",
publisher = "Lippincott Williams & Wilkins, Philadelphia",
journal = "Journal of Strength and Conditioning Research",
title = "Hematological, oxidative stress, and immune status profiling in elite combat sport athletes",
volume = "27",
number = "12",
pages = "3506-3514",
doi = "10.1519/JSC.0b013e31828ddeea"
}

Dopsaj, V., Martinović, J., Dopsaj, M., Kasum, G., Kotur-Stevuljević, J.,& Koropanovski, N.. (2013). Hematological, oxidative stress, and immune status profiling in elite combat sport athletes. in Journal of Strength and Conditioning Research
Lippincott Williams & Wilkins, Philadelphia., 27(12), 3506-3514.
https://doi.org/10.1519/JSC.0b013e31828ddeea

Dopsaj V, Martinović J, Dopsaj M, Kasum G, Kotur-Stevuljević J, Koropanovski N. Hematological, oxidative stress, and immune status profiling in elite combat sport athletes. in Journal of Strength and Conditioning Research. 2013;27(12):3506-3514.
doi:10.1519/JSC.0b013e31828ddeea .

Dopsaj, Violeta, Martinović, Jelena, Dopsaj, Milivoj, Kasum, Goran, Kotur-Stevuljević, Jelena, Koropanovski, Nenad, "Hematological, oxidative stress, and immune status profiling in elite combat sport athletes" in Journal of Strength and Conditioning Research, 27, no. 12 (2013):3506-3514,
https://doi.org/10.1519/JSC.0b013e31828ddeea . .

TY  - JOUR
AU  - Dopsaj, Violeta
AU  - Miković-Golubović, Gordana
AU  - Martinović, Jelena
AU  - Kalimanovska-Oštrić, Dimitra
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1672
AB  - Introduction: The aim of our study was to evaluate derived red blood cell parameters in determining the presence of iron depletion and iron-deficient erythropoiesis, as states that precede iron deficiency anemia, in adults with congenital heart disease. Methods: Eighty-eight adults who were diagnosed with congenital heart disease were divided into two groups (cyanotic and acyanotic). In both groups, congenital heart disease patients were then divided into three subgroups: with iron depletion, with iron-deficient erythropoiesis, and a control group. The following parameters were measured: complete blood count, reticulocytes, ferritin, soluble transferrin receptor, haptoglobin, lactate dehydrogenase, and calculated parameters: low hemoglobin density (LHD), red cell size factor (RSF), and microcytic anemia factor (MAF). Results: Discriminant analysis indicated statistically significant differences in the first discriminant function: Function 1 body iron, LHD, MAF, sTfR, and RSF (P  lt  0.001) in patients with acyanotic congenital heart disease and significant differences in both discriminant functions in patients with cyanotic congenital heart disease: Function 1 body iron, soluble transferrin receptor, LHD, RSF, MAF, lactate dehydrogenase, and haptoglobin (P = 0.008) and Function 2 reticulocytes (#), immature reticulocyte fraction and reticulocytes (%) (P = 0.049). Conclusions: Beside parameters that describe iron metabolism dynamics (body iron and soluble transferrin receptor), LHD, indicator of hypochromia, have the highest potential to differentiate and classify iron deficiency in patients with congenital heart disease.
PB  - Wiley-Blackwell, Hoboken
T2  - International Journal of Laboratory Hematology
T1  - Evaluation of derived Coulter red blood cell parameters for the assessment of iron deficiency in adults with congenital heart disease
VL  - 34
IS  - 5
SP  - 461
EP  - 472
DO  - 10.1111/j.1751-553X.2012.01417.x
ER  - 

@article{
author = "Dopsaj, Violeta and Miković-Golubović, Gordana and Martinović, Jelena and Kalimanovska-Oštrić, Dimitra",
year = "2012",
abstract = "Introduction: The aim of our study was to evaluate derived red blood cell parameters in determining the presence of iron depletion and iron-deficient erythropoiesis, as states that precede iron deficiency anemia, in adults with congenital heart disease. Methods: Eighty-eight adults who were diagnosed with congenital heart disease were divided into two groups (cyanotic and acyanotic). In both groups, congenital heart disease patients were then divided into three subgroups: with iron depletion, with iron-deficient erythropoiesis, and a control group. The following parameters were measured: complete blood count, reticulocytes, ferritin, soluble transferrin receptor, haptoglobin, lactate dehydrogenase, and calculated parameters: low hemoglobin density (LHD), red cell size factor (RSF), and microcytic anemia factor (MAF). Results: Discriminant analysis indicated statistically significant differences in the first discriminant function: Function 1 body iron, LHD, MAF, sTfR, and RSF (P  lt  0.001) in patients with acyanotic congenital heart disease and significant differences in both discriminant functions in patients with cyanotic congenital heart disease: Function 1 body iron, soluble transferrin receptor, LHD, RSF, MAF, lactate dehydrogenase, and haptoglobin (P = 0.008) and Function 2 reticulocytes (#), immature reticulocyte fraction and reticulocytes (%) (P = 0.049). Conclusions: Beside parameters that describe iron metabolism dynamics (body iron and soluble transferrin receptor), LHD, indicator of hypochromia, have the highest potential to differentiate and classify iron deficiency in patients with congenital heart disease.",
publisher = "Wiley-Blackwell, Hoboken",
journal = "International Journal of Laboratory Hematology",
title = "Evaluation of derived Coulter red blood cell parameters for the assessment of iron deficiency in adults with congenital heart disease",
volume = "34",
number = "5",
pages = "461-472",
doi = "10.1111/j.1751-553X.2012.01417.x"
}

Dopsaj, V., Miković-Golubović, G., Martinović, J.,& Kalimanovska-Oštrić, D.. (2012). Evaluation of derived Coulter red blood cell parameters for the assessment of iron deficiency in adults with congenital heart disease. in International Journal of Laboratory Hematology
Wiley-Blackwell, Hoboken., 34(5), 461-472.
https://doi.org/10.1111/j.1751-553X.2012.01417.x

Dopsaj V, Miković-Golubović G, Martinović J, Kalimanovska-Oštrić D. Evaluation of derived Coulter red blood cell parameters for the assessment of iron deficiency in adults with congenital heart disease. in International Journal of Laboratory Hematology. 2012;34(5):461-472.
doi:10.1111/j.1751-553X.2012.01417.x .

Dopsaj, Violeta, Miković-Golubović, Gordana, Martinović, Jelena, Kalimanovska-Oštrić, Dimitra, "Evaluation of derived Coulter red blood cell parameters for the assessment of iron deficiency in adults with congenital heart disease" in International Journal of Laboratory Hematology, 34, no. 5 (2012):461-472,
https://doi.org/10.1111/j.1751-553X.2012.01417.x . .