TY  - JOUR
AU  - Nikolić, Ljubinka
AU  - Čakić, Saša
AU  - Perunović, Neda
AU  - Čolak, Emina
AU  - Kotur-Stevuljević, Jelena
AU  - Janković, Saša
AU  - Đurić, Milanko
AU  - Plećaš, Darko
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3590
AB  - Bacground/Aim. Preterm birth is defined as a delivery prior to the completed 37th week of gestation. Literature data suggested that periodontal processes may influence to the feto-placental unit and induce preterm delivery. The degree of the periodontal disease is influenced by secretor status. Pro-inflammatory cytokines are involved in periodontitis as well as in delivery. The combined influence of these factors on the risk of preterm birth has not been explored. The aim of our study was to investigate the associations between periodontal diseases, secretor status, and interleukin-1-β (IL1-ß) and prostaglandine E2 (PGE2) levels in women delivered preterm. Methods. The study included 56 preterm delivery women and 56 women delivered at term as a control group, aged between 17 and 41 years. Periodontal examination, blood and saliva sampling were performed within 48 hours following delivery. Secretor phenotype was determined by hemagglutination inhibition method. The concentrations of IL1-ß and PGE2 were measured by high sensitivity Enzyme-linked Immunosorbent Assay (ELISA). Results. In the pre-term birth group there were 66.1% of women with periodontitis, while in the control one there were 12.5% (p < 0.01). Concentrations of IL1-ß and PGE2 in plasma were significantly higher in the non-secretor group of women who gave birth pre-term and had periodontitis comparing to other groups. There was a significant correlation between salivary and plasma levels of PGE2 and IL1-ß in the preterm birth group (R = 0.416, p = 0.017 and R = -0.592, p < 0.001, respectively). There were no such correlations in women who delivered at term. Conclusion. Our results support the hypothesis that non-secretor phenotype and periodontitis are at least in part responsible for pathogenesis of preterm birth. This probability of negative impact of non-secretor status cannot be ignored. These findings support the need for additional research into the biology of human parturition. © 2020 Inst. Sci. inf., Univ. Defence in Belgrade. All rights reserved.
AB  - Uvod/Cilj. Prevremeni porođaj se definiše kao porođaj pre navršene 37 nedelje gestacije. Podaci iz literature govore u prilog tome da periodontalni procesi mogu uticati na fetoplacentalnu jedinicu i indukovati preterminski porođaj. Sekretorni status može uticati na stepen periodontalne bolesti. Proinflamatorni citokini imaju uticaj na periodontitis kao i na porođaj. Kombinovani uticaj ovih faktora rizika za prevremeni porođaj nije dovoljno istražen. Cilj ove studije je bio da istraži povezanost između periodontalne bolesti, sekretornog statusa, nivoa interleukina 1-b (IL1-b) i prostaglandina E2 (PGE2) kod žena koje su imale prevremeni porođaj. Metode. Studijom je bilo obuhvaćeno 56 žena, koje su imale prevremen porođaj i 56 žena u kontrolnoj grupi koje su se porodile u terminu, starosti između 17 i 41 godine. Periodontalni pregled, uzorkovanje krvi i salive je izvršeno u prvih 48 sati po porođaju. Sekretorni status je određen metodom inhibicije hemaglutinacije. Koncentracije IL1-b i PGE2 su merene visoko senzitivnim Enzyme-linked Immunosorbent Assay (ELISA) testom. Rezultati. U grupi prevremenih porođaja bilo je 66,1% žena sa periodontitisom, a u kontrolnoj grupi 12,5% (p < 0.01). Prevremeno porođene žena, nesekretori sa periodontitisom imale su u plazmi značajno više vrednosti IL 1-b i PGE 2 u odnosu na ostale grupe (p < 0,01). U grupi prevremeno porođenih žena postojala je značajna korelacija između salivarnih i plazmatskih koncentracija PGE2 i IL1-b (R = 0.416, p = 0.017 i R = -0,592, p < 0,001, redom). Ove korelacije nisu postojale kod žena koje su imale terminski porođaj. Zaključak. Naši rezultati podržavaju hipotezu da su sekretorni status i periodontitis, bar delimično, odgovorni za patogenezu preterminskog porođaja. Verovatnoća negativnog uticaja nesekretornog statusa se ne sme ignorisati. Ovi zaključci ukazuju na potrebu za dodatnim istraživanjima porođaja.
PB  - Beograd : Vojnomedicinska akademija - Institut za naučne informacije
T2  - Vojnosanitetski pregled
T1  - Salivary and plasma inflammatory mediators and secretory status in preterm delivery women with periodontitis - a cross sectional study
T1  - Salivarni i inflamatorni medijatori plazme i sekretorni status prevremeno porođenih žena sa periodontitisom – studija preseka
VL  - 77
IS  - 3
SP  - 247
EP  - 255
DO  - 10.2298/VSP171106066N
ER  - 

@article{
author = "Nikolić, Ljubinka and Čakić, Saša and Perunović, Neda and Čolak, Emina and Kotur-Stevuljević, Jelena and Janković, Saša and Đurić, Milanko and Plećaš, Darko",
year = "2020",
abstract = "Bacground/Aim. Preterm birth is defined as a delivery prior to the completed 37th week of gestation. Literature data suggested that periodontal processes may influence to the feto-placental unit and induce preterm delivery. The degree of the periodontal disease is influenced by secretor status. Pro-inflammatory cytokines are involved in periodontitis as well as in delivery. The combined influence of these factors on the risk of preterm birth has not been explored. The aim of our study was to investigate the associations between periodontal diseases, secretor status, and interleukin-1-β (IL1-ß) and prostaglandine E2 (PGE2) levels in women delivered preterm. Methods. The study included 56 preterm delivery women and 56 women delivered at term as a control group, aged between 17 and 41 years. Periodontal examination, blood and saliva sampling were performed within 48 hours following delivery. Secretor phenotype was determined by hemagglutination inhibition method. The concentrations of IL1-ß and PGE2 were measured by high sensitivity Enzyme-linked Immunosorbent Assay (ELISA). Results. In the pre-term birth group there were 66.1% of women with periodontitis, while in the control one there were 12.5% (p < 0.01). Concentrations of IL1-ß and PGE2 in plasma were significantly higher in the non-secretor group of women who gave birth pre-term and had periodontitis comparing to other groups. There was a significant correlation between salivary and plasma levels of PGE2 and IL1-ß in the preterm birth group (R = 0.416, p = 0.017 and R = -0.592, p < 0.001, respectively). There were no such correlations in women who delivered at term. Conclusion. Our results support the hypothesis that non-secretor phenotype and periodontitis are at least in part responsible for pathogenesis of preterm birth. This probability of negative impact of non-secretor status cannot be ignored. These findings support the need for additional research into the biology of human parturition. © 2020 Inst. Sci. inf., Univ. Defence in Belgrade. All rights reserved., Uvod/Cilj. Prevremeni porođaj se definiše kao porođaj pre navršene 37 nedelje gestacije. Podaci iz literature govore u prilog tome da periodontalni procesi mogu uticati na fetoplacentalnu jedinicu i indukovati preterminski porođaj. Sekretorni status može uticati na stepen periodontalne bolesti. Proinflamatorni citokini imaju uticaj na periodontitis kao i na porođaj. Kombinovani uticaj ovih faktora rizika za prevremeni porođaj nije dovoljno istražen. Cilj ove studije je bio da istraži povezanost između periodontalne bolesti, sekretornog statusa, nivoa interleukina 1-b (IL1-b) i prostaglandina E2 (PGE2) kod žena koje su imale prevremeni porođaj. Metode. Studijom je bilo obuhvaćeno 56 žena, koje su imale prevremen porođaj i 56 žena u kontrolnoj grupi koje su se porodile u terminu, starosti između 17 i 41 godine. Periodontalni pregled, uzorkovanje krvi i salive je izvršeno u prvih 48 sati po porođaju. Sekretorni status je određen metodom inhibicije hemaglutinacije. Koncentracije IL1-b i PGE2 su merene visoko senzitivnim Enzyme-linked Immunosorbent Assay (ELISA) testom. Rezultati. U grupi prevremenih porođaja bilo je 66,1% žena sa periodontitisom, a u kontrolnoj grupi 12,5% (p < 0.01). Prevremeno porođene žena, nesekretori sa periodontitisom imale su u plazmi značajno više vrednosti IL 1-b i PGE 2 u odnosu na ostale grupe (p < 0,01). U grupi prevremeno porođenih žena postojala je značajna korelacija između salivarnih i plazmatskih koncentracija PGE2 i IL1-b (R = 0.416, p = 0.017 i R = -0,592, p < 0,001, redom). Ove korelacije nisu postojale kod žena koje su imale terminski porođaj. Zaključak. Naši rezultati podržavaju hipotezu da su sekretorni status i periodontitis, bar delimično, odgovorni za patogenezu preterminskog porođaja. Verovatnoća negativnog uticaja nesekretornog statusa se ne sme ignorisati. Ovi zaključci ukazuju na potrebu za dodatnim istraživanjima porođaja.",
publisher = "Beograd : Vojnomedicinska akademija - Institut za naučne informacije",
journal = "Vojnosanitetski pregled",
title = "Salivary and plasma inflammatory mediators and secretory status in preterm delivery women with periodontitis - a cross sectional study, Salivarni i inflamatorni medijatori plazme i sekretorni status prevremeno porođenih žena sa periodontitisom – studija preseka",
volume = "77",
number = "3",
pages = "247-255",
doi = "10.2298/VSP171106066N"
}

Nikolić, L., Čakić, S., Perunović, N., Čolak, E., Kotur-Stevuljević, J., Janković, S., Đurić, M.,& Plećaš, D.. (2020). Salivary and plasma inflammatory mediators and secretory status in preterm delivery women with periodontitis - a cross sectional study. in Vojnosanitetski pregled
Beograd : Vojnomedicinska akademija - Institut za naučne informacije., 77(3), 247-255.
https://doi.org/10.2298/VSP171106066N

Nikolić L, Čakić S, Perunović N, Čolak E, Kotur-Stevuljević J, Janković S, Đurić M, Plećaš D. Salivary and plasma inflammatory mediators and secretory status in preterm delivery women with periodontitis - a cross sectional study. in Vojnosanitetski pregled. 2020;77(3):247-255.
doi:10.2298/VSP171106066N .

Nikolić, Ljubinka, Čakić, Saša, Perunović, Neda, Čolak, Emina, Kotur-Stevuljević, Jelena, Janković, Saša, Đurić, Milanko, Plećaš, Darko, "Salivary and plasma inflammatory mediators and secretory status in preterm delivery women with periodontitis - a cross sectional study" in Vojnosanitetski pregled, 77, no. 3 (2020):247-255,
https://doi.org/10.2298/VSP171106066N . .

TY  - JOUR
AU  - Taso, Ervin
AU  - Rakić, Mia
AU  - Bajić, Vladimir
AU  - Petković-Ćurčin, Aleksandra
AU  - Stanojević, Ivan
AU  - Đukić, Mirjana
AU  - Struillou, Xavier
AU  - Vojvodić, Danilo
AU  - Banović, Tatjana
AU  - Kanjevac, Tatjana
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3794
AB  - Profiling of biomarkers of physiological process represents an integrative part in optimisation of diagnostic markers in order to adjust the diagnostic ranges to the potential effects of the local factors such occlusal forces in case of periodontal tissues. The objective of this study was estimation of IL-1β, IL-2, IL-4, IL-5, IL- 6, IL-9, IL-10, IL-12, IL-13, IL-17, IL-22, TNFα and IFNγ concentrations in gingival crevicular fluid samples (GCF) between different groups of teeth. Two hundred fifty-nine systemically healthy non-smokers having at least one vital tooth without restorations, with healthy periodontal tissues, were clinically examined and the GCF sample was retrieved. The cytokine levels were estimated using flow cytometry and compared between central incisors (CI), lateral incisors, canines, first premolars, second premolars, first molars and second molars. Cytokine profiles varied between different groups of teeth with tendency of increase in proinflammatory cytokines from anterior teeth toward molars. Molars might be considered teeth with natural predisposition for faster bone resorption while the adjustment of diagnostic range of periodontal biomarkers for anterior or posterior teeth should be considered within diagnostic context. Cytokine profiles varied between different groups of teeth with tendency of increase in proinflammatory cytokines from anterior teeth toward molars. Molars might be considered teeth with natural predisposition for faster bone resorption while the adjustment of diagnostic range of periodontal biomarkers for anterior or posterior teeth should be considered within diagnostic context.
AB  - Profilisanje biomarkera fiziološkog procesa predstavlja integrativni deo optimalizacije dijagnostičkih markera, kako bi se dijagnostički rasponi prilagodili potencijalnim uticajima lokalnih faktora poput okluzijskih sila u slučaju parodontalnih tkiva. Cilj ove studije bila je procena koncentracija IL-1b, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12, IL-13, IL-17, IL- 22, TNFα i IFNγ u uzorcima gingivalne tečnosti (GT) kod različitih grupa zuba. Klinički je pregledano dvesta pedeset devet sistemski zdravih nepušača sa najmanje jednim vitalnim zubom bez restauracija, sa zdravim parodontalnim tkivima, i uzet je GT uzorak. Nivoi citokina procenjeni su protočnom citometrijom i upoređeni između centralnih sekutića (CS), bočnih sekutića, očnjaka, prvih i drugih premolara, kao i prvih i drugih kutnjaka. Profil citokina varirao je između različitih grupa zuba sa tendencijom povećanja pro-upalnih citokina od prednjih zuba do kutnjaka. Molari se mogu smatrati zubima sa prirodnom predispozicijom za bržu resorpciju kosti, dok bi podešavanje dijagnostičkog raspona parodontalnih biomarkera za prednje ili zadnje zube trebalo razmotriti unutar dijagnostičkog konteksta.
PB  - University of Kragujevac, Faculty of Science
T2  - Serbian Journal of Experimental and Clinical Research
T1  - Variation of the cytokine profiles in gingival crevicular fluid between different groups of periodontally healthy teeth
T1  - Varijacija profila citokina u gingivalnoj zglobnoj tečnosti između različitih grupa parodontalno zdravih zuba
VL  - 21
IS  - 4
SP  - 333
EP  - 341
DO  - 10.2478/sjecr-2019-0015
ER  - 

@article{
author = "Taso, Ervin and Rakić, Mia and Bajić, Vladimir and Petković-Ćurčin, Aleksandra and Stanojević, Ivan and Đukić, Mirjana and Struillou, Xavier and Vojvodić, Danilo and Banović, Tatjana and Kanjevac, Tatjana",
year = "2020",
abstract = "Profiling of biomarkers of physiological process represents an integrative part in optimisation of diagnostic markers in order to adjust the diagnostic ranges to the potential effects of the local factors such occlusal forces in case of periodontal tissues. The objective of this study was estimation of IL-1β, IL-2, IL-4, IL-5, IL- 6, IL-9, IL-10, IL-12, IL-13, IL-17, IL-22, TNFα and IFNγ concentrations in gingival crevicular fluid samples (GCF) between different groups of teeth. Two hundred fifty-nine systemically healthy non-smokers having at least one vital tooth without restorations, with healthy periodontal tissues, were clinically examined and the GCF sample was retrieved. The cytokine levels were estimated using flow cytometry and compared between central incisors (CI), lateral incisors, canines, first premolars, second premolars, first molars and second molars. Cytokine profiles varied between different groups of teeth with tendency of increase in proinflammatory cytokines from anterior teeth toward molars. Molars might be considered teeth with natural predisposition for faster bone resorption while the adjustment of diagnostic range of periodontal biomarkers for anterior or posterior teeth should be considered within diagnostic context. Cytokine profiles varied between different groups of teeth with tendency of increase in proinflammatory cytokines from anterior teeth toward molars. Molars might be considered teeth with natural predisposition for faster bone resorption while the adjustment of diagnostic range of periodontal biomarkers for anterior or posterior teeth should be considered within diagnostic context., Profilisanje biomarkera fiziološkog procesa predstavlja integrativni deo optimalizacije dijagnostičkih markera, kako bi se dijagnostički rasponi prilagodili potencijalnim uticajima lokalnih faktora poput okluzijskih sila u slučaju parodontalnih tkiva. Cilj ove studije bila je procena koncentracija IL-1b, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12, IL-13, IL-17, IL- 22, TNFα i IFNγ u uzorcima gingivalne tečnosti (GT) kod različitih grupa zuba. Klinički je pregledano dvesta pedeset devet sistemski zdravih nepušača sa najmanje jednim vitalnim zubom bez restauracija, sa zdravim parodontalnim tkivima, i uzet je GT uzorak. Nivoi citokina procenjeni su protočnom citometrijom i upoređeni između centralnih sekutića (CS), bočnih sekutića, očnjaka, prvih i drugih premolara, kao i prvih i drugih kutnjaka. Profil citokina varirao je između različitih grupa zuba sa tendencijom povećanja pro-upalnih citokina od prednjih zuba do kutnjaka. Molari se mogu smatrati zubima sa prirodnom predispozicijom za bržu resorpciju kosti, dok bi podešavanje dijagnostičkog raspona parodontalnih biomarkera za prednje ili zadnje zube trebalo razmotriti unutar dijagnostičkog konteksta.",
publisher = "University of Kragujevac, Faculty of Science",
journal = "Serbian Journal of Experimental and Clinical Research",
title = "Variation of the cytokine profiles in gingival crevicular fluid between different groups of periodontally healthy teeth, Varijacija profila citokina u gingivalnoj zglobnoj tečnosti između različitih grupa parodontalno zdravih zuba",
volume = "21",
number = "4",
pages = "333-341",
doi = "10.2478/sjecr-2019-0015"
}

Taso, E., Rakić, M., Bajić, V., Petković-Ćurčin, A., Stanojević, I., Đukić, M., Struillou, X., Vojvodić, D., Banović, T.,& Kanjevac, T.. (2020). Variation of the cytokine profiles in gingival crevicular fluid between different groups of periodontally healthy teeth. in Serbian Journal of Experimental and Clinical Research
University of Kragujevac, Faculty of Science., 21(4), 333-341.
https://doi.org/10.2478/sjecr-2019-0015

Taso E, Rakić M, Bajić V, Petković-Ćurčin A, Stanojević I, Đukić M, Struillou X, Vojvodić D, Banović T, Kanjevac T. Variation of the cytokine profiles in gingival crevicular fluid between different groups of periodontally healthy teeth. in Serbian Journal of Experimental and Clinical Research. 2020;21(4):333-341.
doi:10.2478/sjecr-2019-0015 .

Taso, Ervin, Rakić, Mia, Bajić, Vladimir, Petković-Ćurčin, Aleksandra, Stanojević, Ivan, Đukić, Mirjana, Struillou, Xavier, Vojvodić, Danilo, Banović, Tatjana, Kanjevac, Tatjana, "Variation of the cytokine profiles in gingival crevicular fluid between different groups of periodontally healthy teeth" in Serbian Journal of Experimental and Clinical Research, 21, no. 4 (2020):333-341,
https://doi.org/10.2478/sjecr-2019-0015 . .

TY  - JOUR
AU  - Stefanović, Vladimir
AU  - Taso, Ervin
AU  - Petković-Ćurčin, Aleksandra
AU  - Đukić, Mirjana
AU  - Gardašević, Milka
AU  - Rakić, Mia
AU  - Xavier, Struillou
AU  - Jović, Milena
AU  - Miller, Karolina
AU  - Stanojević, Ivan
AU  - Vojvodić, Danilo
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2668
AB  - Background/Aim. Several cytokines and lymphokines (IL1β, ENA78, IL6, TNFα, IL8 and S100A8) are expressed during dental pulp inflammation. Analysis of gingival crevicular fluid (GCF) offers a non-invasive means of studying general host response in oral cavity. Although GCF levels of various mediators could reflect the state of inflammation both in dental pulp and gingiva adjacent to a tooth, GCF samples of those without significant gingivitis could be interpreted as reflection of pulpal process. The aim of this study was to investigate IL9 GCF values in patients with dental caries and to assess possible influence of various dental fillings materials on local IL9 production. Methods. The study group included 90 patients, aged 18-70, with inclusion and exclusion criteria in the prospective clinical study. Of the 6 types of material used for the restoration of prepared cavities, 3 were intended for temporary and 3 for definitive restoration. According to dental fillings weight, all the participants were divided into 3 groups: those with fillings lighter than 0.50 g, those with 0.50-1.00 g, and those with fillings heavier than 1.00 g. Samples were taken from gingival sulcus using the filter paper technique. Clinical parameters were determined by bleeding index, plaque index (Silness-Lou, 0-3), gingival index (0-3), and gingival sulcus depth. Cytokine concentrations were assessed using commercially available cytomix. Results. According to the weight of dental fillings, there was a clear decreament trend of IL9 values meaning that dental defects greater than 1.00 g of dental filling were associated with lower GCF IL9 concentration. The IL9 values correlated with the degree of gingival index and depth of gingival sulcus, being higher with more advanced gingivitis and more pronounced anatomical changes in the tooth edge. Different filling materials exerted various local IL9 responses. Zink polycarbonate cement and amalgam fillings induced a significant and long-lasting local IL9 decrement, while the use of Tetric EvoCeram and GMA-BISK significantly increased IL9 levels. Conclusion. The obtained results indicate that IL9 GCF could be regarded as a measure of odontoblasts' response to the extensity of dental caries. The type of material used for dental fillings could profoundly alter biological function of gingival and pulpal cells. Also, the results obtained in this study suggest that some materials could even enhance wound repair by modulating macrophage activation.
AB  - Uvod/Cilj. Jedan broj citokina i limfokina (IL1β, ENA78, IL6, TNFα, IL8 I S100A8) izlučuje se tokom upale zubne pulpe. Analiza gingivalne sulkusne tečnosti (gingival crevicular fluid - GCF) omogućava neinvazivno proučavanje opšteg odgovora pacijenta na lokalne promene u usnoj duplji. Iako nivoi GCF mogu da odražavaju stanje upale kako u zubnoj pulpi, tako i u gingivi susednog zuba, uzorci GCF zuba bez značajnijeg gingivitisa mogli bi se tumačiti kao pokazatelji procesa u pulpi. Cilj ovog istraživanja bio je da se ispitaju nivoi IL9 u GCF kod pacijenata sa zubnim karijesom i da se utvrdi eventualni uticaj različitih materijala za zubne ispune na lokalnu proizvodnju IL9. Metode. U studiju je bilo uključeno 90 pacijenata, starih od 18 do 70 godina, uz primenu kriterijuma za uključivanje/isključivanje za prospektivne kliničke studije. Od ukupno šest materijala za punjenje pripremljenih kaviteta, tri je korišćeno za privremenu i tri za trajnu ispunu. Prema težini zubnog ispuna, pacijenti su bili podeljeni na tri grupe: oni sa ispunama lakšim od 0,50 g, sa ispunama od 0,50 do 1,00 g i sa ispunama težim od 1,00 g. Uzorci su uzimani iz gingivalnog sulkusa primenom tehnike filter papira. Korišćeni klinički parametri bili su indeks krvarenja, plak indeks (Silness-Lou, 0-3), gingivalni indeks (0-3) i dubina gingivalnog sulkusa. Koncentracije citokina određene su komercijalnim citomiksom. Rezultati. Težina zubne ispune ukazivala je na tendenciju opadanja vrednosti IL9, što je značilo da je veće oštećenje zuba, sa zubnom ispunom težom od 1,00 g, praćeno nižom koncentracijom IL9 u GCF. Vrednosti IL9 bile su u korelaciji sa stepenom gingivalnog indeksa i dubinom gingivalnog sulkusa, pogotovu u poodmaklom gingivitisu i izraženijim anatomskim promenama ivica zuba. Različite ispune izazivale su različite lokalne sekrecije IL9. Cink-polikarbonatni cement i amalgamska ispuna izazvale su značajan i dugotrajan pad nivoa lokalnog IL9, dok je primena tetrik-evocerama i GMA-BISK znatno povisila nivoe IL9. Zaključak. Rezultati dobijeni u ovoj studiji ukazuju da se IL9 u GCF može koristiti kao mera za reakciju odontoblasta na veličinu karijesa. Tip zubne ispune može da promeni biološku funkciju ćelija gingive i pulpe. Rezultati ove studije, takođe, ukazuju i na to da neke vrste ispuna mogu čak da ubrzaju zarastanje rane modulacijom aktivnosti makrofaga.
PB  - Vojnomedicinska akademija - Institut za naučne informacije, Beograd
T2  - Vojnosanitetski pregled
T1  - Influence of dental filling material type on the concentration of interleukin 9 in the samples of gingival crevicular fluid
T1  - Uticaj tipa materijala za plombiranje zuba na koncentraciju interleukina 9 u uzorcima gingivalne sulkusne tečnosti
VL  - 73
IS  - 8
SP  - 728
EP  - 734
DO  - 10.2298/VSP140227054S
ER  - 

@article{
author = "Stefanović, Vladimir and Taso, Ervin and Petković-Ćurčin, Aleksandra and Đukić, Mirjana and Gardašević, Milka and Rakić, Mia and Xavier, Struillou and Jović, Milena and Miller, Karolina and Stanojević, Ivan and Vojvodić, Danilo",
year = "2016",
abstract = "Background/Aim. Several cytokines and lymphokines (IL1β, ENA78, IL6, TNFα, IL8 and S100A8) are expressed during dental pulp inflammation. Analysis of gingival crevicular fluid (GCF) offers a non-invasive means of studying general host response in oral cavity. Although GCF levels of various mediators could reflect the state of inflammation both in dental pulp and gingiva adjacent to a tooth, GCF samples of those without significant gingivitis could be interpreted as reflection of pulpal process. The aim of this study was to investigate IL9 GCF values in patients with dental caries and to assess possible influence of various dental fillings materials on local IL9 production. Methods. The study group included 90 patients, aged 18-70, with inclusion and exclusion criteria in the prospective clinical study. Of the 6 types of material used for the restoration of prepared cavities, 3 were intended for temporary and 3 for definitive restoration. According to dental fillings weight, all the participants were divided into 3 groups: those with fillings lighter than 0.50 g, those with 0.50-1.00 g, and those with fillings heavier than 1.00 g. Samples were taken from gingival sulcus using the filter paper technique. Clinical parameters were determined by bleeding index, plaque index (Silness-Lou, 0-3), gingival index (0-3), and gingival sulcus depth. Cytokine concentrations were assessed using commercially available cytomix. Results. According to the weight of dental fillings, there was a clear decreament trend of IL9 values meaning that dental defects greater than 1.00 g of dental filling were associated with lower GCF IL9 concentration. The IL9 values correlated with the degree of gingival index and depth of gingival sulcus, being higher with more advanced gingivitis and more pronounced anatomical changes in the tooth edge. Different filling materials exerted various local IL9 responses. Zink polycarbonate cement and amalgam fillings induced a significant and long-lasting local IL9 decrement, while the use of Tetric EvoCeram and GMA-BISK significantly increased IL9 levels. Conclusion. The obtained results indicate that IL9 GCF could be regarded as a measure of odontoblasts' response to the extensity of dental caries. The type of material used for dental fillings could profoundly alter biological function of gingival and pulpal cells. Also, the results obtained in this study suggest that some materials could even enhance wound repair by modulating macrophage activation., Uvod/Cilj. Jedan broj citokina i limfokina (IL1β, ENA78, IL6, TNFα, IL8 I S100A8) izlučuje se tokom upale zubne pulpe. Analiza gingivalne sulkusne tečnosti (gingival crevicular fluid - GCF) omogućava neinvazivno proučavanje opšteg odgovora pacijenta na lokalne promene u usnoj duplji. Iako nivoi GCF mogu da odražavaju stanje upale kako u zubnoj pulpi, tako i u gingivi susednog zuba, uzorci GCF zuba bez značajnijeg gingivitisa mogli bi se tumačiti kao pokazatelji procesa u pulpi. Cilj ovog istraživanja bio je da se ispitaju nivoi IL9 u GCF kod pacijenata sa zubnim karijesom i da se utvrdi eventualni uticaj različitih materijala za zubne ispune na lokalnu proizvodnju IL9. Metode. U studiju je bilo uključeno 90 pacijenata, starih od 18 do 70 godina, uz primenu kriterijuma za uključivanje/isključivanje za prospektivne kliničke studije. Od ukupno šest materijala za punjenje pripremljenih kaviteta, tri je korišćeno za privremenu i tri za trajnu ispunu. Prema težini zubnog ispuna, pacijenti su bili podeljeni na tri grupe: oni sa ispunama lakšim od 0,50 g, sa ispunama od 0,50 do 1,00 g i sa ispunama težim od 1,00 g. Uzorci su uzimani iz gingivalnog sulkusa primenom tehnike filter papira. Korišćeni klinički parametri bili su indeks krvarenja, plak indeks (Silness-Lou, 0-3), gingivalni indeks (0-3) i dubina gingivalnog sulkusa. Koncentracije citokina određene su komercijalnim citomiksom. Rezultati. Težina zubne ispune ukazivala je na tendenciju opadanja vrednosti IL9, što je značilo da je veće oštećenje zuba, sa zubnom ispunom težom od 1,00 g, praćeno nižom koncentracijom IL9 u GCF. Vrednosti IL9 bile su u korelaciji sa stepenom gingivalnog indeksa i dubinom gingivalnog sulkusa, pogotovu u poodmaklom gingivitisu i izraženijim anatomskim promenama ivica zuba. Različite ispune izazivale su različite lokalne sekrecije IL9. Cink-polikarbonatni cement i amalgamska ispuna izazvale su značajan i dugotrajan pad nivoa lokalnog IL9, dok je primena tetrik-evocerama i GMA-BISK znatno povisila nivoe IL9. Zaključak. Rezultati dobijeni u ovoj studiji ukazuju da se IL9 u GCF može koristiti kao mera za reakciju odontoblasta na veličinu karijesa. Tip zubne ispune može da promeni biološku funkciju ćelija gingive i pulpe. Rezultati ove studije, takođe, ukazuju i na to da neke vrste ispuna mogu čak da ubrzaju zarastanje rane modulacijom aktivnosti makrofaga.",
publisher = "Vojnomedicinska akademija - Institut za naučne informacije, Beograd",
journal = "Vojnosanitetski pregled",
title = "Influence of dental filling material type on the concentration of interleukin 9 in the samples of gingival crevicular fluid, Uticaj tipa materijala za plombiranje zuba na koncentraciju interleukina 9 u uzorcima gingivalne sulkusne tečnosti",
volume = "73",
number = "8",
pages = "728-734",
doi = "10.2298/VSP140227054S"
}

Stefanović, V., Taso, E., Petković-Ćurčin, A., Đukić, M., Gardašević, M., Rakić, M., Xavier, S., Jović, M., Miller, K., Stanojević, I.,& Vojvodić, D.. (2016). Influence of dental filling material type on the concentration of interleukin 9 in the samples of gingival crevicular fluid. in Vojnosanitetski pregled
Vojnomedicinska akademija - Institut za naučne informacije, Beograd., 73(8), 728-734.
https://doi.org/10.2298/VSP140227054S

Stefanović V, Taso E, Petković-Ćurčin A, Đukić M, Gardašević M, Rakić M, Xavier S, Jović M, Miller K, Stanojević I, Vojvodić D. Influence of dental filling material type on the concentration of interleukin 9 in the samples of gingival crevicular fluid. in Vojnosanitetski pregled. 2016;73(8):728-734.
doi:10.2298/VSP140227054S .

Stefanović, Vladimir, Taso, Ervin, Petković-Ćurčin, Aleksandra, Đukić, Mirjana, Gardašević, Milka, Rakić, Mia, Xavier, Struillou, Jović, Milena, Miller, Karolina, Stanojević, Ivan, Vojvodić, Danilo, "Influence of dental filling material type on the concentration of interleukin 9 in the samples of gingival crevicular fluid" in Vojnosanitetski pregled, 73, no. 8 (2016):728-734,
https://doi.org/10.2298/VSP140227054S . .