TY  - JOUR
AU  - Đekić, Ljiljana
AU  - Čalija, Bojan
AU  - Medarević, Đorđe
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3527
AB  - The study elucidated the combined effect of the formulation parameters (the relative contents of the copolymer (poloxamer 407 (P407)), the cosolvent (isopropyl alcohol), and the hydrophobic drug (ibuprofen)) on gelation, applicative properties, drug solubilization capacity and release kinetics of the P407 aqueous solutions under the common conditions of storage and topical administration. The presence of ibuprofen at a therapeutic concentration of 5% enhanced the increase in micellar volume fraction and allowed the gelation of the liquid solutions at P407 concentrations ≥ 15%. Light microscopy, DSC analysis, and rheological measurements pointed that P407 gels with 15–20% of the copolymer enabled controled precipitation of amorphous ibuprofen particles (≤100 μm) in perimicellar microchannels, while gels with 25–30% of P407 as well as increased relative content of the cosolvent in perimicellar aqueous phase, had capacity for complete ibuprofen solubilization. The dissolution of the drug particles during the in vitro drug release test, maintained the drug concentration gradient between the perimicellar microchannels and the acceptor medium allowing diffusion-based sustained drug release up to 12 h. The gels with completely solubilized drug notably decrease drug release rate and the cumulative amount of the drug released. Harmonization of the investigated formulation parameters was critical to the formulation of P407 gels that could be promising drug delivery systems for sustained percutaneous delivery of the hydrophobic model drug ibuprofen with reduced frequency of administration and improved patient compliance.
PB  - Elsevier B.V.
T2  - Journal of Molecular Liquids
T1  - Gelation behavior, drug solubilization capacity and release kinetics of poloxamer 407 aqueous solutions: The combined effect of copolymer, cosolvent and hydrophobic drug
VL  - 303
DO  - 10.1016/j.molliq.2020.112639
ER  - 

@article{
author = "Đekić, Ljiljana and Čalija, Bojan and Medarević, Đorđe",
year = "2020",
abstract = "The study elucidated the combined effect of the formulation parameters (the relative contents of the copolymer (poloxamer 407 (P407)), the cosolvent (isopropyl alcohol), and the hydrophobic drug (ibuprofen)) on gelation, applicative properties, drug solubilization capacity and release kinetics of the P407 aqueous solutions under the common conditions of storage and topical administration. The presence of ibuprofen at a therapeutic concentration of 5% enhanced the increase in micellar volume fraction and allowed the gelation of the liquid solutions at P407 concentrations ≥ 15%. Light microscopy, DSC analysis, and rheological measurements pointed that P407 gels with 15–20% of the copolymer enabled controled precipitation of amorphous ibuprofen particles (≤100 μm) in perimicellar microchannels, while gels with 25–30% of P407 as well as increased relative content of the cosolvent in perimicellar aqueous phase, had capacity for complete ibuprofen solubilization. The dissolution of the drug particles during the in vitro drug release test, maintained the drug concentration gradient between the perimicellar microchannels and the acceptor medium allowing diffusion-based sustained drug release up to 12 h. The gels with completely solubilized drug notably decrease drug release rate and the cumulative amount of the drug released. Harmonization of the investigated formulation parameters was critical to the formulation of P407 gels that could be promising drug delivery systems for sustained percutaneous delivery of the hydrophobic model drug ibuprofen with reduced frequency of administration and improved patient compliance.",
publisher = "Elsevier B.V.",
journal = "Journal of Molecular Liquids",
title = "Gelation behavior, drug solubilization capacity and release kinetics of poloxamer 407 aqueous solutions: The combined effect of copolymer, cosolvent and hydrophobic drug",
volume = "303",
doi = "10.1016/j.molliq.2020.112639"
}

Đekić, L., Čalija, B.,& Medarević, Đ.. (2020). Gelation behavior, drug solubilization capacity and release kinetics of poloxamer 407 aqueous solutions: The combined effect of copolymer, cosolvent and hydrophobic drug. in Journal of Molecular Liquids
Elsevier B.V.., 303.
https://doi.org/10.1016/j.molliq.2020.112639

Đekić L, Čalija B, Medarević Đ. Gelation behavior, drug solubilization capacity and release kinetics of poloxamer 407 aqueous solutions: The combined effect of copolymer, cosolvent and hydrophobic drug. in Journal of Molecular Liquids. 2020;303.
doi:10.1016/j.molliq.2020.112639 .

Đekić, Ljiljana, Čalija, Bojan, Medarević, Đorđe, "Gelation behavior, drug solubilization capacity and release kinetics of poloxamer 407 aqueous solutions: The combined effect of copolymer, cosolvent and hydrophobic drug" in Journal of Molecular Liquids, 303 (2020),
https://doi.org/10.1016/j.molliq.2020.112639 . .

TY  - JOUR
AU  - Ćirić, Ana
AU  - Medarević, Đorđe
AU  - Čalija, Bojan
AU  - Dobričić, Vladimir
AU  - Mitrić, Miodrag
AU  - Đekić, Ljiljana
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3524
AB  - This study investigated the combined influence of pH adjusting agent type (hydrochloric, acetic or lactic acid) and initial pH value (3.6, 4.6, and 5.6) on formation of biocompatible chitosan/xanthan polyelectrolyte complexes (PECs), their characteristics in solid state and influence on in vitro ibuprofen release kinetics. Conductivity measurements and rheological characterization revealed generally higher extent of ionic interactions in PEC dispersions comprising acetic acid and at pH 3.6. Acid type and pH affected significantly the yield and particle size (100–250 μm) of the dried PECs. Differential scanning calorimetry (DSC), Fourier-transform infrared spectroscopy (FT-IR), and powder X-ray diffraction (PXRD) analysis of the solid PECs confirmed exclusively physical (ionic, hydrogen bonds) interactions between chitosan and xanthan gum. PECs prepared with acetic acid at pH 4.6 and 5.6 had enhanced rehydration ability in phosphate buffer pH 7.2, and at PEC-to-drug mass ratio up to 1:2, enabled extended ibuprofen release from hard capsules during 10 h.
PB  - Elsevier B.V.
T2  - International Journal of Biological Macromolecules
T1  - Study of chitosan/xanthan gum polyelectrolyte complexes formation, solid state and influence on ibuprofen release kinetics
VL  - 148
SP  - 942
EP  - 955
DO  - 10.1016/j.ijbiomac.2020.01.138
ER  - 

@article{
author = "Ćirić, Ana and Medarević, Đorđe and Čalija, Bojan and Dobričić, Vladimir and Mitrić, Miodrag and Đekić, Ljiljana",
year = "2020",
abstract = "This study investigated the combined influence of pH adjusting agent type (hydrochloric, acetic or lactic acid) and initial pH value (3.6, 4.6, and 5.6) on formation of biocompatible chitosan/xanthan polyelectrolyte complexes (PECs), their characteristics in solid state and influence on in vitro ibuprofen release kinetics. Conductivity measurements and rheological characterization revealed generally higher extent of ionic interactions in PEC dispersions comprising acetic acid and at pH 3.6. Acid type and pH affected significantly the yield and particle size (100–250 μm) of the dried PECs. Differential scanning calorimetry (DSC), Fourier-transform infrared spectroscopy (FT-IR), and powder X-ray diffraction (PXRD) analysis of the solid PECs confirmed exclusively physical (ionic, hydrogen bonds) interactions between chitosan and xanthan gum. PECs prepared with acetic acid at pH 4.6 and 5.6 had enhanced rehydration ability in phosphate buffer pH 7.2, and at PEC-to-drug mass ratio up to 1:2, enabled extended ibuprofen release from hard capsules during 10 h.",
publisher = "Elsevier B.V.",
journal = "International Journal of Biological Macromolecules",
title = "Study of chitosan/xanthan gum polyelectrolyte complexes formation, solid state and influence on ibuprofen release kinetics",
volume = "148",
pages = "942-955",
doi = "10.1016/j.ijbiomac.2020.01.138"
}

Ćirić, A., Medarević, Đ., Čalija, B., Dobričić, V., Mitrić, M.,& Đekić, L.. (2020). Study of chitosan/xanthan gum polyelectrolyte complexes formation, solid state and influence on ibuprofen release kinetics. in International Journal of Biological Macromolecules
Elsevier B.V.., 148, 942-955.
https://doi.org/10.1016/j.ijbiomac.2020.01.138

Ćirić A, Medarević Đ, Čalija B, Dobričić V, Mitrić M, Đekić L. Study of chitosan/xanthan gum polyelectrolyte complexes formation, solid state and influence on ibuprofen release kinetics. in International Journal of Biological Macromolecules. 2020;148:942-955.
doi:10.1016/j.ijbiomac.2020.01.138 .

Ćirić, Ana, Medarević, Đorđe, Čalija, Bojan, Dobričić, Vladimir, Mitrić, Miodrag, Đekić, Ljiljana, "Study of chitosan/xanthan gum polyelectrolyte complexes formation, solid state and influence on ibuprofen release kinetics" in International Journal of Biological Macromolecules, 148 (2020):942-955,
https://doi.org/10.1016/j.ijbiomac.2020.01.138 . .

TY  - JOUR
AU  - Čalija, Bojan
AU  - Milić, Jela
AU  - Milašinović, Nikola
AU  - Daković, Aleksandra
AU  - Trifković, Kata
AU  - Stojanović, Jovica
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3478
AB  - This study was designed to investigate functionality of tetracycline‐loaded chitosan‐halloysite nanocomposite films, with focus on evaluating the influence of chitosan molar mass on films applicability for sustained local antibiotic delivery. The films were prepared by casting and solvent evaporation using low, medium, and high molar mass chitosan. SEM analysis revealed compact, nonporous and rough surface of the nanocomposite films due to the presence of halloysite agglomerates and tetracycline crystals. Increasing chitosan molar mass led to higher values of elongation at break (from 21.65 ± 2.65 to 34.48 ± 2.34%), tensile strength (from 134.8 ± 13.21 to 246.36 ± 14.69 MPa), and elastic modulus (from 633.79 ± 128.37 to 716.55 ± 60.76 MPa) of the nanocomposite films. FT‐IR, XRPD, and thermal analyses confirmed molar mass dependent chitosan‐halloysite interactions and improved thermal stability of the nanocomposite films in comparison with chitosan films. The nanocomposite films released tetracycline in a sustained manner, with the slowest release achieved from the films consisting of low molar mass chitosan. Chitosan molar mass was confirmed to be a functionality‐related characteristic of chitosan‐halloysite nanocomposite films as potential sustained‐release carriers for topical delivery of antibiotics
PB  - Wiley Periodicals, Inc.
T2  - Journal of Applied Polymer Science
T1  - Functionality of chitosan‐halloysite nanocomposite films for sustained delivery of antibiotics: The effect of chitosan molar mass
VL  - 137
IS  - 8
DO  - 10.1002/app.48406
ER  - 

@article{
author = "Čalija, Bojan and Milić, Jela and Milašinović, Nikola and Daković, Aleksandra and Trifković, Kata and Stojanović, Jovica",
year = "2020",
abstract = "This study was designed to investigate functionality of tetracycline‐loaded chitosan‐halloysite nanocomposite films, with focus on evaluating the influence of chitosan molar mass on films applicability for sustained local antibiotic delivery. The films were prepared by casting and solvent evaporation using low, medium, and high molar mass chitosan. SEM analysis revealed compact, nonporous and rough surface of the nanocomposite films due to the presence of halloysite agglomerates and tetracycline crystals. Increasing chitosan molar mass led to higher values of elongation at break (from 21.65 ± 2.65 to 34.48 ± 2.34%), tensile strength (from 134.8 ± 13.21 to 246.36 ± 14.69 MPa), and elastic modulus (from 633.79 ± 128.37 to 716.55 ± 60.76 MPa) of the nanocomposite films. FT‐IR, XRPD, and thermal analyses confirmed molar mass dependent chitosan‐halloysite interactions and improved thermal stability of the nanocomposite films in comparison with chitosan films. The nanocomposite films released tetracycline in a sustained manner, with the slowest release achieved from the films consisting of low molar mass chitosan. Chitosan molar mass was confirmed to be a functionality‐related characteristic of chitosan‐halloysite nanocomposite films as potential sustained‐release carriers for topical delivery of antibiotics",
publisher = "Wiley Periodicals, Inc.",
journal = "Journal of Applied Polymer Science",
title = "Functionality of chitosan‐halloysite nanocomposite films for sustained delivery of antibiotics: The effect of chitosan molar mass",
volume = "137",
number = "8",
doi = "10.1002/app.48406"
}

Čalija, B., Milić, J., Milašinović, N., Daković, A., Trifković, K.,& Stojanović, J.. (2020). Functionality of chitosan‐halloysite nanocomposite films for sustained delivery of antibiotics: The effect of chitosan molar mass. in Journal of Applied Polymer Science
Wiley Periodicals, Inc.., 137(8).
https://doi.org/10.1002/app.48406

Čalija B, Milić J, Milašinović N, Daković A, Trifković K, Stojanović J. Functionality of chitosan‐halloysite nanocomposite films for sustained delivery of antibiotics: The effect of chitosan molar mass. in Journal of Applied Polymer Science. 2020;137(8).
doi:10.1002/app.48406 .

Čalija, Bojan, Milić, Jela, Milašinović, Nikola, Daković, Aleksandra, Trifković, Kata, Stojanović, Jovica, "Functionality of chitosan‐halloysite nanocomposite films for sustained delivery of antibiotics: The effect of chitosan molar mass" in Journal of Applied Polymer Science, 137, no. 8 (2020),
https://doi.org/10.1002/app.48406 . .

TY  - JOUR
AU  - Đekić, Ljiljana
AU  - Martinović, Martina
AU  - Ćirić, Ana
AU  - Fraj, Jadranka
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5372
AB  - The physical chitosan hydrogel, obtained by ionic gelation in lactic acid solution, was combined with biocompatible oil-in-water microemulsion with ibuprofen, to prepare composite hydrogels with 0.25–1% of the polymer and 5% of the drug. The electrical conductivity measurement, photon correlation spectroscopy (PCS), and rheological analysis showed that the composite hydrogels comprise oil nanodroplets (16.21–22.56 nm) embedded within pseudoplastic chitosan hydrogel. In vitro ibuprofen release was sustained for 12 h and followed zero-order kinetics. pH values of the composite hydrogels were in the range of 4.80–5.27, thus physiologically acceptable. The formulation containing 0.5% chitosan enabled the maximum drug release rate of 239.25 μgh−1cm−2 as well as cohesiveness (154.958 ± 0.731 g*s) higher than hardness (13.546 ± 0.065 g) and adhesiveness (−12.042 ± 1.161 g*s), so textural properties were suitable for application along skin surface, without spillage, and for easy removal. This is the first study in which the composite chitosan hydrogels with ibuprofen were formulated by combining the chitosan hydrogel prepared without harmful chemical crosslinkers and low viscosity oil-in-water microemulsion, and the preclinical characterization of their biopharmaceutical aspect and textural characterization, that is of key importance in improving the patient’s compliance, were performed.
PB  - Taylor & Francis
T2  - Pharmaceutical Development and Technology
T1  - Composite chitosan hydrogels as advanced wound dressings with sustained ibuprofen release and suitable application characteristics
VL  - 25
IS  - 3
SP  - 332
EP  - 339
DO  - 10.1080/10837450.2019.1701495
ER  - 

@article{
author = "Đekić, Ljiljana and Martinović, Martina and Ćirić, Ana and Fraj, Jadranka",
year = "2020",
abstract = "The physical chitosan hydrogel, obtained by ionic gelation in lactic acid solution, was combined with biocompatible oil-in-water microemulsion with ibuprofen, to prepare composite hydrogels with 0.25–1% of the polymer and 5% of the drug. The electrical conductivity measurement, photon correlation spectroscopy (PCS), and rheological analysis showed that the composite hydrogels comprise oil nanodroplets (16.21–22.56 nm) embedded within pseudoplastic chitosan hydrogel. In vitro ibuprofen release was sustained for 12 h and followed zero-order kinetics. pH values of the composite hydrogels were in the range of 4.80–5.27, thus physiologically acceptable. The formulation containing 0.5% chitosan enabled the maximum drug release rate of 239.25 μgh−1cm−2 as well as cohesiveness (154.958 ± 0.731 g*s) higher than hardness (13.546 ± 0.065 g) and adhesiveness (−12.042 ± 1.161 g*s), so textural properties were suitable for application along skin surface, without spillage, and for easy removal. This is the first study in which the composite chitosan hydrogels with ibuprofen were formulated by combining the chitosan hydrogel prepared without harmful chemical crosslinkers and low viscosity oil-in-water microemulsion, and the preclinical characterization of their biopharmaceutical aspect and textural characterization, that is of key importance in improving the patient’s compliance, were performed.",
publisher = "Taylor & Francis",
journal = "Pharmaceutical Development and Technology",
title = "Composite chitosan hydrogels as advanced wound dressings with sustained ibuprofen release and suitable application characteristics",
volume = "25",
number = "3",
pages = "332-339",
doi = "10.1080/10837450.2019.1701495"
}

Đekić, L., Martinović, M., Ćirić, A.,& Fraj, J.. (2020). Composite chitosan hydrogels as advanced wound dressings with sustained ibuprofen release and suitable application characteristics. in Pharmaceutical Development and Technology
Taylor & Francis., 25(3), 332-339.
https://doi.org/10.1080/10837450.2019.1701495

Đekić L, Martinović M, Ćirić A, Fraj J. Composite chitosan hydrogels as advanced wound dressings with sustained ibuprofen release and suitable application characteristics. in Pharmaceutical Development and Technology. 2020;25(3):332-339.
doi:10.1080/10837450.2019.1701495 .

Đekić, Ljiljana, Martinović, Martina, Ćirić, Ana, Fraj, Jadranka, "Composite chitosan hydrogels as advanced wound dressings with sustained ibuprofen release and suitable application characteristics" in Pharmaceutical Development and Technology, 25, no. 3 (2020):332-339,
https://doi.org/10.1080/10837450.2019.1701495 . .

TY  - JOUR
AU  - Čalija, Bojan
AU  - Milić, Jela
AU  - Milašinović, Nikola
AU  - Daković, Aleksandra
AU  - Trifković, Kata
AU  - Stojanović, Jovica
AU  - Krajišnik, Danina
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3476
AB  - This study was designed to investigate functionality of tetracycline‐loaded chitosan‐halloysite nanocomposite films, with focus on evaluating the influence of chitosan molar mass on films applicability for sustained local antibiotic delivery. The films were prepared by casting and solvent evaporation using low, medium, and high molar mass chitosan. SEM analysis revealed compact, nonporous and rough surface of the nanocomposite films due to the presence of halloysite agglomerates and tetracycline crystals. Increasing chitosan molar mass led to higher values of elongation at break (from 21.65 ± 2.65 to 34.48 ± 2.34%), tensile strength (from 134.8 ± 13.21 to 246.36 ± 14.69 MPa), and elastic modulus (from 633.79 ± 128.37 to 716.55 ± 60.76 MPa) of the nanocomposite films. FT‐IR, XRPD, and thermal analyses confirmed molar mass dependent chitosan‐halloysite interactions and improved thermal stability of the nanocomposite films in comparison with chitosan films. The nanocomposite films released tetracycline in a sustained manner, with the slowest release achieved from the films consisting of low molar mass chitosan. Chitosan molar mass was confirmed to be a functionality‐related characteristic of chitosan‐halloysite nanocomposite films as potential sustained‐release carriers for topical delivery of antibiotics.
PB  - Wiley Periodicals, Inc.
T2  - Journal of Applied Polymer Science
T1  - Functionality of chitosan‐halloysite nanocomposite films for sustained delivery of antibiotics: The effect of chitosan molar mass
VL  - 137
IS  - 8
DO  - 10.1002/app.48406
ER  - 

@article{
author = "Čalija, Bojan and Milić, Jela and Milašinović, Nikola and Daković, Aleksandra and Trifković, Kata and Stojanović, Jovica and Krajišnik, Danina",
year = "2020",
abstract = "This study was designed to investigate functionality of tetracycline‐loaded chitosan‐halloysite nanocomposite films, with focus on evaluating the influence of chitosan molar mass on films applicability for sustained local antibiotic delivery. The films were prepared by casting and solvent evaporation using low, medium, and high molar mass chitosan. SEM analysis revealed compact, nonporous and rough surface of the nanocomposite films due to the presence of halloysite agglomerates and tetracycline crystals. Increasing chitosan molar mass led to higher values of elongation at break (from 21.65 ± 2.65 to 34.48 ± 2.34%), tensile strength (from 134.8 ± 13.21 to 246.36 ± 14.69 MPa), and elastic modulus (from 633.79 ± 128.37 to 716.55 ± 60.76 MPa) of the nanocomposite films. FT‐IR, XRPD, and thermal analyses confirmed molar mass dependent chitosan‐halloysite interactions and improved thermal stability of the nanocomposite films in comparison with chitosan films. The nanocomposite films released tetracycline in a sustained manner, with the slowest release achieved from the films consisting of low molar mass chitosan. Chitosan molar mass was confirmed to be a functionality‐related characteristic of chitosan‐halloysite nanocomposite films as potential sustained‐release carriers for topical delivery of antibiotics.",
publisher = "Wiley Periodicals, Inc.",
journal = "Journal of Applied Polymer Science",
title = "Functionality of chitosan‐halloysite nanocomposite films for sustained delivery of antibiotics: The effect of chitosan molar mass",
volume = "137",
number = "8",
doi = "10.1002/app.48406"
}

Čalija, B., Milić, J., Milašinović, N., Daković, A., Trifković, K., Stojanović, J.,& Krajišnik, D.. (2020). Functionality of chitosan‐halloysite nanocomposite films for sustained delivery of antibiotics: The effect of chitosan molar mass. in Journal of Applied Polymer Science
Wiley Periodicals, Inc.., 137(8).
https://doi.org/10.1002/app.48406

Čalija B, Milić J, Milašinović N, Daković A, Trifković K, Stojanović J, Krajišnik D. Functionality of chitosan‐halloysite nanocomposite films for sustained delivery of antibiotics: The effect of chitosan molar mass. in Journal of Applied Polymer Science. 2020;137(8).
doi:10.1002/app.48406 .

Čalija, Bojan, Milić, Jela, Milašinović, Nikola, Daković, Aleksandra, Trifković, Kata, Stojanović, Jovica, Krajišnik, Danina, "Functionality of chitosan‐halloysite nanocomposite films for sustained delivery of antibiotics: The effect of chitosan molar mass" in Journal of Applied Polymer Science, 137, no. 8 (2020),
https://doi.org/10.1002/app.48406 . .

TY  - CONF
AU  - Milutinović, Violeta
AU  - Petrović, Predrag
AU  - Klaus, Anita
AU  - Ušjak, Ljuboš
AU  - Niketić, Marjan
AU  - Petrović, Silvana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5110
AB  - The ability of the dried MeOH extracts of aerial flowering parts of 28 Hieracium s. str. species from Balkan Peninsula and their selected metabolites (seven flavonoids, three phenolic acids and two sesquiterpene lactones) to inhibit acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) was determined using colorimetric Ellman method. The study included: H. gymnocephalum, H. orieni, H. blecicii, H. paratrichum, H. spirocaule, H. mokragorae, H. pannosum s.l., H. plumulosum, H. villosum, H. pilosum, H. pseudoschenkii, H. naegelianum, H. anastrum, H. calophyllum, H. scheppigianum, H. durmitoricum, H. guentheri-beckii, H. mirificissimum, H. coloriscapum, H. pyricephalum, H. albopellitum, H. glabratum, H. scorzonerifolium s.l., H. dentatum s.l., H. neilreichii, H. valdepilosum s.l., H. tommasinianum and H. macrodontoides. The extracts were mainly more active towards AChE, i.e. all inhibited more than 50% AChE, with H. pseudoschenkii extract being the most potent (IC50=0.64 mg/mL). Seven extracts reached 50% inhibition of 
BuChE, and H. pilosum extract was the most active (IC50=0.56 mg/mL). The observed activity could be attributed to some tested constituents. Flavonoid aglycones apigenin, luteolin and diosmetin significantly inhibited both enzymes (IC50AChE=47.12-89.89 μg/mL; C50BuChE=18.40-73.44 μg/mL). Sesquiterpene lactone 8-epiixerisamine A selectively inhibited AChE (IC50=80.01 μg/mL). The other tested metabolites did not reach 50% inhibition of both enzymes.
PB  - Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš
PB  - Institute for Nature Conservation of Serbia
C3  - 13th Symposium on the Flora of  Southeastern Serbia  and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts
T1  - Acetylcholinesterase and butyrylcholinesterase inhibitory activity of methanol extracts of 28 Hieracium species and their selected metabolites
SP  - 180
EP  - 180
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5110
ER  - 

@conference{
author = "Milutinović, Violeta and Petrović, Predrag and Klaus, Anita and Ušjak, Ljuboš and Niketić, Marjan and Petrović, Silvana",
year = "2019",
abstract = "The ability of the dried MeOH extracts of aerial flowering parts of 28 Hieracium s. str. species from Balkan Peninsula and their selected metabolites (seven flavonoids, three phenolic acids and two sesquiterpene lactones) to inhibit acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) was determined using colorimetric Ellman method. The study included: H. gymnocephalum, H. orieni, H. blecicii, H. paratrichum, H. spirocaule, H. mokragorae, H. pannosum s.l., H. plumulosum, H. villosum, H. pilosum, H. pseudoschenkii, H. naegelianum, H. anastrum, H. calophyllum, H. scheppigianum, H. durmitoricum, H. guentheri-beckii, H. mirificissimum, H. coloriscapum, H. pyricephalum, H. albopellitum, H. glabratum, H. scorzonerifolium s.l., H. dentatum s.l., H. neilreichii, H. valdepilosum s.l., H. tommasinianum and H. macrodontoides. The extracts were mainly more active towards AChE, i.e. all inhibited more than 50% AChE, with H. pseudoschenkii extract being the most potent (IC50=0.64 mg/mL). Seven extracts reached 50% inhibition of 
BuChE, and H. pilosum extract was the most active (IC50=0.56 mg/mL). The observed activity could be attributed to some tested constituents. Flavonoid aglycones apigenin, luteolin and diosmetin significantly inhibited both enzymes (IC50AChE=47.12-89.89 μg/mL; C50BuChE=18.40-73.44 μg/mL). Sesquiterpene lactone 8-epiixerisamine A selectively inhibited AChE (IC50=80.01 μg/mL). The other tested metabolites did not reach 50% inhibition of both enzymes.",
publisher = "Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš, Institute for Nature Conservation of Serbia",
journal = "13th Symposium on the Flora of  Southeastern Serbia  and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts",
title = "Acetylcholinesterase and butyrylcholinesterase inhibitory activity of methanol extracts of 28 Hieracium species and their selected metabolites",
pages = "180-180",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5110"
}

Milutinović, V., Petrović, P., Klaus, A., Ušjak, L., Niketić, M.,& Petrović, S.. (2019). Acetylcholinesterase and butyrylcholinesterase inhibitory activity of methanol extracts of 28 Hieracium species and their selected metabolites. in 13th Symposium on the Flora of  Southeastern Serbia  and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts
Department of Biology and Ecology, Faculty of Sciences and Mathematics, University of Niš., 180-180.
https://hdl.handle.net/21.15107/rcub_farfar_5110

Milutinović V, Petrović P, Klaus A, Ušjak L, Niketić M, Petrović S. Acetylcholinesterase and butyrylcholinesterase inhibitory activity of methanol extracts of 28 Hieracium species and their selected metabolites. in 13th Symposium on the Flora of  Southeastern Serbia  and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts. 2019;:180-180.
https://hdl.handle.net/21.15107/rcub_farfar_5110 .

Milutinović, Violeta, Petrović, Predrag, Klaus, Anita, Ušjak, Ljuboš, Niketić, Marjan, Petrović, Silvana, "Acetylcholinesterase and butyrylcholinesterase inhibitory activity of methanol extracts of 28 Hieracium species and their selected metabolites" in 13th Symposium on the Flora of  Southeastern Serbia  and Neighboring Regions, 20th to 23th June, 2019 - Book of Abstracts (2019):180-180,
https://hdl.handle.net/21.15107/rcub_farfar_5110 .

TY  - JOUR
AU  - Petrović, Predrag
AU  - Ivanović, Katarina
AU  - Jovanović, Aleksandra
AU  - Simović, Milica
AU  - Milutinović, Violeta
AU  - Kozarski, Maja
AU  - Petković, Miloš
AU  - Cvetković, Anka
AU  - Klaus, Anita
AU  - Bugarski, Branko
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5105
AB  - Puffballs are fungi that produce globose fruiting bodies that undergo autolysis, transforming their insides into a spore bearing, powdery mass. Mature fruiting bodies are traditionally used to treat open skin wounds. In this study, methanol extracts of two puffball species, Handkea excipuliformis and Vascellum pratense, were examined and compared in order to provide insight into the changes these mushrooms undergo during maturation, with respect to their potential use in skin care and wound treatment. Some compounds involved in skin care and regeneration were quantified, and it was found that maturation increases the concentrations of almost all of these compounds. Antioxidant activity was also more pronounced in mature fruiting body extracts, which was in correlation with the higher content of antioxidants. Tyrosinase inhibition was vastly improved with autolysis, correlating with the higher phenolic content in mature fruiting body extracts. Antimicrobial activity was negatively affected by autolysis in the case of H. excipuliformis, whereas autolysis had little effect on the antimicrobial activity of V. pratense. Autolysis generally improved the biological activity and increased the concentrations of compounds involved in skin care, which justifies the traditional use of puffballs and makes them good candidates for various potential cosmetic and medicinal skin-care products.
PB  - Serbian Biological Society
T2  - Archives Biological Sciences
T1  - The impact of puffball autolysis on selected chemical and biological properties: puffball extracts as potential ingredients of skin-care products
VL  - 71
IS  - 4
SP  - 721
EP  - 733
DO  - 10.2298/ABS190725055P
ER  - 

@article{
author = "Petrović, Predrag and Ivanović, Katarina and Jovanović, Aleksandra and Simović, Milica and Milutinović, Violeta and Kozarski, Maja and Petković, Miloš and Cvetković, Anka and Klaus, Anita and Bugarski, Branko",
year = "2019",
abstract = "Puffballs are fungi that produce globose fruiting bodies that undergo autolysis, transforming their insides into a spore bearing, powdery mass. Mature fruiting bodies are traditionally used to treat open skin wounds. In this study, methanol extracts of two puffball species, Handkea excipuliformis and Vascellum pratense, were examined and compared in order to provide insight into the changes these mushrooms undergo during maturation, with respect to their potential use in skin care and wound treatment. Some compounds involved in skin care and regeneration were quantified, and it was found that maturation increases the concentrations of almost all of these compounds. Antioxidant activity was also more pronounced in mature fruiting body extracts, which was in correlation with the higher content of antioxidants. Tyrosinase inhibition was vastly improved with autolysis, correlating with the higher phenolic content in mature fruiting body extracts. Antimicrobial activity was negatively affected by autolysis in the case of H. excipuliformis, whereas autolysis had little effect on the antimicrobial activity of V. pratense. Autolysis generally improved the biological activity and increased the concentrations of compounds involved in skin care, which justifies the traditional use of puffballs and makes them good candidates for various potential cosmetic and medicinal skin-care products.",
publisher = "Serbian Biological Society",
journal = "Archives Biological Sciences",
title = "The impact of puffball autolysis on selected chemical and biological properties: puffball extracts as potential ingredients of skin-care products",
volume = "71",
number = "4",
pages = "721-733",
doi = "10.2298/ABS190725055P"
}

Petrović, P., Ivanović, K., Jovanović, A., Simović, M., Milutinović, V., Kozarski, M., Petković, M., Cvetković, A., Klaus, A.,& Bugarski, B.. (2019). The impact of puffball autolysis on selected chemical and biological properties: puffball extracts as potential ingredients of skin-care products. in Archives Biological Sciences
Serbian Biological Society., 71(4), 721-733.
https://doi.org/10.2298/ABS190725055P

Petrović P, Ivanović K, Jovanović A, Simović M, Milutinović V, Kozarski M, Petković M, Cvetković A, Klaus A, Bugarski B. The impact of puffball autolysis on selected chemical and biological properties: puffball extracts as potential ingredients of skin-care products. in Archives Biological Sciences. 2019;71(4):721-733.
doi:10.2298/ABS190725055P .

Petrović, Predrag, Ivanović, Katarina, Jovanović, Aleksandra, Simović, Milica, Milutinović, Violeta, Kozarski, Maja, Petković, Miloš, Cvetković, Anka, Klaus, Anita, Bugarski, Branko, "The impact of puffball autolysis on selected chemical and biological properties: puffball extracts as potential ingredients of skin-care products" in Archives Biological Sciences, 71, no. 4 (2019):721-733,
https://doi.org/10.2298/ABS190725055P . .

TY  - CONF
AU  - Ćirić, Ana
AU  - Mitrić, Miodrag
AU  - Dobričić, Vladimir
AU  - Đekić, Ljiljana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5366
AB  - Polyelectrolyte complexes are novel drug delivery systems obtained by establishing ion interactions between two oppositely charged polymers. ...
PB  - University of Novi Sad, Faculty of Technology Novi Sad
C3  - 1st International Conference on Advanced Production and Processing 10th-11th October 2019 Novi Sad, Serbia (Book of Abstracts)
T1  - Influence of drying method on chitosan/xanthan polyelectrolyte complex characteristics
SP  - 185
EP  - 185
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5366
ER  - 

@conference{
author = "Ćirić, Ana and Mitrić, Miodrag and Dobričić, Vladimir and Đekić, Ljiljana",
year = "2019",
abstract = "Polyelectrolyte complexes are novel drug delivery systems obtained by establishing ion interactions between two oppositely charged polymers. ...",
publisher = "University of Novi Sad, Faculty of Technology Novi Sad",
journal = "1st International Conference on Advanced Production and Processing 10th-11th October 2019 Novi Sad, Serbia (Book of Abstracts)",
title = "Influence of drying method on chitosan/xanthan polyelectrolyte complex characteristics",
pages = "185-185",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5366"
}

Ćirić, A., Mitrić, M., Dobričić, V.,& Đekić, L.. (2019). Influence of drying method on chitosan/xanthan polyelectrolyte complex characteristics. in 1st International Conference on Advanced Production and Processing 10th-11th October 2019 Novi Sad, Serbia (Book of Abstracts)
University of Novi Sad, Faculty of Technology Novi Sad., 185-185.
https://hdl.handle.net/21.15107/rcub_farfar_5366

Ćirić A, Mitrić M, Dobričić V, Đekić L. Influence of drying method on chitosan/xanthan polyelectrolyte complex characteristics. in 1st International Conference on Advanced Production and Processing 10th-11th October 2019 Novi Sad, Serbia (Book of Abstracts). 2019;:185-185.
https://hdl.handle.net/21.15107/rcub_farfar_5366 .

Ćirić, Ana, Mitrić, Miodrag, Dobričić, Vladimir, Đekić, Ljiljana, "Influence of drying method on chitosan/xanthan polyelectrolyte complex characteristics" in 1st International Conference on Advanced Production and Processing 10th-11th October 2019 Novi Sad, Serbia (Book of Abstracts) (2019):185-185,
https://hdl.handle.net/21.15107/rcub_farfar_5366 .

TY  - CONF
AU  - Ćirić, Ana
AU  - Đekić, Ljiljana
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5367
AB  - Ibuprofen is one of the most frequently used analgesic with good risk/benefit ratio. Due to its short half-life (t1/2 ~ 2 h), frequent administration of immediate release dosage forms is necessary. ...
PB  - University of Novi Sad, Faculty of Technology Novi Sad
C3  - 1st International Conference on Advanced Production and Processing 10th-11th October 2019 Novi Sad, Serbia (Book of Abstracts)
T1  - In vitro characterization of rehydration process and dissolution of ibuprofen from chitosan/xanthan polyelectrolyte complex based drug delivery systems
SP  - 186
EP  - 186
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5367
ER  - 

@conference{
author = "Ćirić, Ana and Đekić, Ljiljana",
year = "2019",
abstract = "Ibuprofen is one of the most frequently used analgesic with good risk/benefit ratio. Due to its short half-life (t1/2 ~ 2 h), frequent administration of immediate release dosage forms is necessary. ...",
publisher = "University of Novi Sad, Faculty of Technology Novi Sad",
journal = "1st International Conference on Advanced Production and Processing 10th-11th October 2019 Novi Sad, Serbia (Book of Abstracts)",
title = "In vitro characterization of rehydration process and dissolution of ibuprofen from chitosan/xanthan polyelectrolyte complex based drug delivery systems",
pages = "186-186",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5367"
}

Ćirić, A.,& Đekić, L.. (2019). In vitro characterization of rehydration process and dissolution of ibuprofen from chitosan/xanthan polyelectrolyte complex based drug delivery systems. in 1st International Conference on Advanced Production and Processing 10th-11th October 2019 Novi Sad, Serbia (Book of Abstracts)
University of Novi Sad, Faculty of Technology Novi Sad., 186-186.
https://hdl.handle.net/21.15107/rcub_farfar_5367

Ćirić A, Đekić L. In vitro characterization of rehydration process and dissolution of ibuprofen from chitosan/xanthan polyelectrolyte complex based drug delivery systems. in 1st International Conference on Advanced Production and Processing 10th-11th October 2019 Novi Sad, Serbia (Book of Abstracts). 2019;:186-186.
https://hdl.handle.net/21.15107/rcub_farfar_5367 .

Ćirić, Ana, Đekić, Ljiljana, "In vitro characterization of rehydration process and dissolution of ibuprofen from chitosan/xanthan polyelectrolyte complex based drug delivery systems" in 1st International Conference on Advanced Production and Processing 10th-11th October 2019 Novi Sad, Serbia (Book of Abstracts) (2019):186-186,
https://hdl.handle.net/21.15107/rcub_farfar_5367 .

TY  - JOUR
AU  - Petrović, Predrag
AU  - Vunduk, J.
AU  - Klaus, A.
AU  - Carević, Momir
AU  - Petković, Miloš
AU  - Vuković, N.
AU  - Cvetković, A.
AU  - Žižak, Željko
AU  - Bugarski, Branko
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3357
AB  - Methanol extracts of mosaic puffball (Handkea utriformis, Bovistella utriformis, Lycoperdon utriforme, Calvatia utriformis - current name is a subject of debate), from three different stages - mycelium (HUMIC), immature (HUI) and mature fruiting bodies (HUM) were characterized and tested for antioxidant, antimicrobial and inhibitory activity on tyrosinase, acetyholinesterase (AChE), butyrylcholinesterase (BuChE) and 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMG-CoA-R). Immature, edible, fruiting bodies were shown to be a good source of antioxidants (11.5 mg/g of extract) and cholesterol-lowering agent, lovastatin (234 mu g/g of extract), and exhibited significant antimicrobial activity. In addition, HUI showed good and selective AChE (4.48 mg/mL) and non-lovastatin related HMG-CoA-R inhibition (1.16 mg/mL), which all together suggests that regular consumption of it may have health benefits. Mature fruiting bodies, inedible due to powdery consistence, have been used in traditional medicine for wound treatment; their extract was relatively rich in free ergosterol (31.65 mg/g of extract), N-acetylglucosamine (24 mg/g of extract), alpha-tocopherol (4 mg/g of extract) and had best overall antioxidant activity, which was in correlation with its highest phenolic content (19.4 mg GAE/mL). It also exhibited significant tyrosinase inhibitory activity (0.22 mg/mL) and thus could be used in medicinal and cosmetic products for wound healing and bleaching. Mycelium, which can be easily grown in laboratory conditions did not have the same properties as, neither immature or mature fruiting bodies, although it showed prominent antimicrobial activity, notably against Pseudomonas aeruginosa (MIC = 0.0625 mg/mL) and could be a source of antimicrobial compounds.
PB  - Elsevier Science BV, Amsterdam
T2  - Southern Medical Journal
T1  - From mycelium to spores: A whole circle of biological potency of mosaic puffball
VL  - 123
SP  - 152
EP  - 160
DO  - 10.1016/j.sajb.2019.03.016
ER  - 

@article{
author = "Petrović, Predrag and Vunduk, J. and Klaus, A. and Carević, Momir and Petković, Miloš and Vuković, N. and Cvetković, A. and Žižak, Željko and Bugarski, Branko",
year = "2019",
abstract = "Methanol extracts of mosaic puffball (Handkea utriformis, Bovistella utriformis, Lycoperdon utriforme, Calvatia utriformis - current name is a subject of debate), from three different stages - mycelium (HUMIC), immature (HUI) and mature fruiting bodies (HUM) were characterized and tested for antioxidant, antimicrobial and inhibitory activity on tyrosinase, acetyholinesterase (AChE), butyrylcholinesterase (BuChE) and 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMG-CoA-R). Immature, edible, fruiting bodies were shown to be a good source of antioxidants (11.5 mg/g of extract) and cholesterol-lowering agent, lovastatin (234 mu g/g of extract), and exhibited significant antimicrobial activity. In addition, HUI showed good and selective AChE (4.48 mg/mL) and non-lovastatin related HMG-CoA-R inhibition (1.16 mg/mL), which all together suggests that regular consumption of it may have health benefits. Mature fruiting bodies, inedible due to powdery consistence, have been used in traditional medicine for wound treatment; their extract was relatively rich in free ergosterol (31.65 mg/g of extract), N-acetylglucosamine (24 mg/g of extract), alpha-tocopherol (4 mg/g of extract) and had best overall antioxidant activity, which was in correlation with its highest phenolic content (19.4 mg GAE/mL). It also exhibited significant tyrosinase inhibitory activity (0.22 mg/mL) and thus could be used in medicinal and cosmetic products for wound healing and bleaching. Mycelium, which can be easily grown in laboratory conditions did not have the same properties as, neither immature or mature fruiting bodies, although it showed prominent antimicrobial activity, notably against Pseudomonas aeruginosa (MIC = 0.0625 mg/mL) and could be a source of antimicrobial compounds.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Southern Medical Journal",
title = "From mycelium to spores: A whole circle of biological potency of mosaic puffball",
volume = "123",
pages = "152-160",
doi = "10.1016/j.sajb.2019.03.016"
}

Petrović, P., Vunduk, J., Klaus, A., Carević, M., Petković, M., Vuković, N., Cvetković, A., Žižak, Ž.,& Bugarski, B.. (2019). From mycelium to spores: A whole circle of biological potency of mosaic puffball. in Southern Medical Journal
Elsevier Science BV, Amsterdam., 123, 152-160.
https://doi.org/10.1016/j.sajb.2019.03.016

Petrović P, Vunduk J, Klaus A, Carević M, Petković M, Vuković N, Cvetković A, Žižak Ž, Bugarski B. From mycelium to spores: A whole circle of biological potency of mosaic puffball. in Southern Medical Journal. 2019;123:152-160.
doi:10.1016/j.sajb.2019.03.016 .

Petrović, Predrag, Vunduk, J., Klaus, A., Carević, Momir, Petković, Miloš, Vuković, N., Cvetković, A., Žižak, Željko, Bugarski, Branko, "From mycelium to spores: A whole circle of biological potency of mosaic puffball" in Southern Medical Journal, 123 (2019):152-160,
https://doi.org/10.1016/j.sajb.2019.03.016 . .

TY  - JOUR
AU  - Đekić, Ljiljana
AU  - Čalija, Bojan
AU  - Micov, Ana
AU  - Tomić, Maja
AU  - Stepanović-Petrović, Radica
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3268
AB  - The physicochemical properties, stability, in vivo antihyperalgesic activity, and skin irritation potential of the carbomer hydrogels with the new chemical entity escin β-sitosterol (ES) phytosome were characterized and compared with those containing escin. Physicochemical characterization of the hydrogels (performed 48 hr after preparation) included organoleptic examination, pH measurement, light microscopy, differential scanning calorimetry analysis and rheological tests. The obtained results showed that increasing concentration of the active substances within 1–5% affected the appearance (color and transparency) of the hydrogels, their pH, consistency, and rheological behavior. Unlike acidic escin, which was dissolved in the liquid phase of the pseudoplastic hydrogels E1–E5 and reduced their maximal apparent viscosity (ηmax), minimal apparent viscosity (ηmin), and hysteresis area (H) in comparison to the plain carbomer hydrogel, amphiphilic ES-enhanced ηmax, ηmin, and thixotropy of the hydrogels ES1–ES5, which is favorable for prolonged retention at skin surface. Evaluation of in-use stability of the hydrogels showed that organoleptic characteristics, flow behavior, and pH values could be preserved for 3 months under ambient conditions. The rat ear test results suggested that the hydrogels are safe to be used on human skin. Both escin and ES-loaded hydrogels exerted significant, concentration-dependent antihyperalgesic effect in inflammatory pain model in rats. ES-loaded hydrogels were significantly more effective than those loaded with escin. This is a first report on the antihyperalgesic effect of topically applied escin as well as ES in a model of inflammatory pain.
PB  - Wiley-Liss Inc.
T2  - Drug Development Research
T1  - Topical hydrogels with escin β-sitosterol phytosome and escin: Formulation development and in vivo assessment of antihyperalgesic activity
DO  - 10.1002/ddr.21572
ER  - 

@article{
author = "Đekić, Ljiljana and Čalija, Bojan and Micov, Ana and Tomić, Maja and Stepanović-Petrović, Radica",
year = "2019",
abstract = "The physicochemical properties, stability, in vivo antihyperalgesic activity, and skin irritation potential of the carbomer hydrogels with the new chemical entity escin β-sitosterol (ES) phytosome were characterized and compared with those containing escin. Physicochemical characterization of the hydrogels (performed 48 hr after preparation) included organoleptic examination, pH measurement, light microscopy, differential scanning calorimetry analysis and rheological tests. The obtained results showed that increasing concentration of the active substances within 1–5% affected the appearance (color and transparency) of the hydrogels, their pH, consistency, and rheological behavior. Unlike acidic escin, which was dissolved in the liquid phase of the pseudoplastic hydrogels E1–E5 and reduced their maximal apparent viscosity (ηmax), minimal apparent viscosity (ηmin), and hysteresis area (H) in comparison to the plain carbomer hydrogel, amphiphilic ES-enhanced ηmax, ηmin, and thixotropy of the hydrogels ES1–ES5, which is favorable for prolonged retention at skin surface. Evaluation of in-use stability of the hydrogels showed that organoleptic characteristics, flow behavior, and pH values could be preserved for 3 months under ambient conditions. The rat ear test results suggested that the hydrogels are safe to be used on human skin. Both escin and ES-loaded hydrogels exerted significant, concentration-dependent antihyperalgesic effect in inflammatory pain model in rats. ES-loaded hydrogels were significantly more effective than those loaded with escin. This is a first report on the antihyperalgesic effect of topically applied escin as well as ES in a model of inflammatory pain.",
publisher = "Wiley-Liss Inc.",
journal = "Drug Development Research",
title = "Topical hydrogels with escin β-sitosterol phytosome and escin: Formulation development and in vivo assessment of antihyperalgesic activity",
doi = "10.1002/ddr.21572"
}

Đekić, L., Čalija, B., Micov, A., Tomić, M.,& Stepanović-Petrović, R.. (2019). Topical hydrogels with escin β-sitosterol phytosome and escin: Formulation development and in vivo assessment of antihyperalgesic activity. in Drug Development Research
Wiley-Liss Inc...
https://doi.org/10.1002/ddr.21572

Đekić L, Čalija B, Micov A, Tomić M, Stepanović-Petrović R. Topical hydrogels with escin β-sitosterol phytosome and escin: Formulation development and in vivo assessment of antihyperalgesic activity. in Drug Development Research. 2019;.
doi:10.1002/ddr.21572 .

Đekić, Ljiljana, Čalija, Bojan, Micov, Ana, Tomić, Maja, Stepanović-Petrović, Radica, "Topical hydrogels with escin β-sitosterol phytosome and escin: Formulation development and in vivo assessment of antihyperalgesic activity" in Drug Development Research (2019),
https://doi.org/10.1002/ddr.21572 . .

TY  - JOUR
AU  - Đekić, Ljiljana
AU  - Martinović, Martina
AU  - Dobričić, Vladimir
AU  - Čalija, Bojan
AU  - Medarević, Đorđe
AU  - Primorac, Marija
PY  - 2019
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3305
AB  - The study is focused on formulation of biocompatible hydrogels with a poorly soluble drug ibuprofen (5%) and comprehensive evaluation and comparison of effect of different bioadhesive polymers on their suitability for application on skin, physical stability during the accelerated and natural aging tests (by performing centrifugation test, light microscopy, differential scanning calorimetry, rheological and pH measurements), and in vitro drug release kinetics. Hydrogels, formulated with xanthan gum 1% (XIB), sodium carboxymethylcellulose 5% (CMCIB), poloxamer 407 16% (PIB), and carbomer 1% (KIB), were soft pseudoplastic semisolids with thixotropy and biocompatible pH. The type of the polymer significantly affected apparent viscosity of the hydrogels and miscibility rate with artificial sweat, their physical stability, and shape, size, and aggregation of the drug crystals and degree of crystallization. The drug release in all investigated hydrogels was diffusion-controlled in accordance with the Higuchi model and sustained for 12 h, with the drug release rate and the amount of drug released depended on the polymer. The described formulation approach enabled discrimination of the hydrogels with unsatisfactory application properties (CMCIB) and physical stability (KIB), and selection of the hydrogel with promising characteristics in terms of all investigated aspects (XIB) which could be considered for further evaluation.
PB  - Elsevier Science Inc, New York
T2  - Journal of Pharmaceutical Sciences
T1  - Comparison of the Effect of Bioadhesive Polymers on Stability and Drug Release Kinetics of Biocompatible Hydrogels for Topical Application of Ibuprofen
VL  - 108
IS  - 3
SP  - 1326
EP  - 1333
DO  - 10.1016/j.xphs.2018.10.054
ER  - 

@article{
author = "Đekić, Ljiljana and Martinović, Martina and Dobričić, Vladimir and Čalija, Bojan and Medarević, Đorđe and Primorac, Marija",
year = "2019",
abstract = "The study is focused on formulation of biocompatible hydrogels with a poorly soluble drug ibuprofen (5%) and comprehensive evaluation and comparison of effect of different bioadhesive polymers on their suitability for application on skin, physical stability during the accelerated and natural aging tests (by performing centrifugation test, light microscopy, differential scanning calorimetry, rheological and pH measurements), and in vitro drug release kinetics. Hydrogels, formulated with xanthan gum 1% (XIB), sodium carboxymethylcellulose 5% (CMCIB), poloxamer 407 16% (PIB), and carbomer 1% (KIB), were soft pseudoplastic semisolids with thixotropy and biocompatible pH. The type of the polymer significantly affected apparent viscosity of the hydrogels and miscibility rate with artificial sweat, their physical stability, and shape, size, and aggregation of the drug crystals and degree of crystallization. The drug release in all investigated hydrogels was diffusion-controlled in accordance with the Higuchi model and sustained for 12 h, with the drug release rate and the amount of drug released depended on the polymer. The described formulation approach enabled discrimination of the hydrogels with unsatisfactory application properties (CMCIB) and physical stability (KIB), and selection of the hydrogel with promising characteristics in terms of all investigated aspects (XIB) which could be considered for further evaluation.",
publisher = "Elsevier Science Inc, New York",
journal = "Journal of Pharmaceutical Sciences",
title = "Comparison of the Effect of Bioadhesive Polymers on Stability and Drug Release Kinetics of Biocompatible Hydrogels for Topical Application of Ibuprofen",
volume = "108",
number = "3",
pages = "1326-1333",
doi = "10.1016/j.xphs.2018.10.054"
}

Đekić, L., Martinović, M., Dobričić, V., Čalija, B., Medarević, Đ.,& Primorac, M.. (2019). Comparison of the Effect of Bioadhesive Polymers on Stability and Drug Release Kinetics of Biocompatible Hydrogels for Topical Application of Ibuprofen. in Journal of Pharmaceutical Sciences
Elsevier Science Inc, New York., 108(3), 1326-1333.
https://doi.org/10.1016/j.xphs.2018.10.054

Đekić L, Martinović M, Dobričić V, Čalija B, Medarević Đ, Primorac M. Comparison of the Effect of Bioadhesive Polymers on Stability and Drug Release Kinetics of Biocompatible Hydrogels for Topical Application of Ibuprofen. in Journal of Pharmaceutical Sciences. 2019;108(3):1326-1333.
doi:10.1016/j.xphs.2018.10.054 .

Đekić, Ljiljana, Martinović, Martina, Dobričić, Vladimir, Čalija, Bojan, Medarević, Đorđe, Primorac, Marija, "Comparison of the Effect of Bioadhesive Polymers on Stability and Drug Release Kinetics of Biocompatible Hydrogels for Topical Application of Ibuprofen" in Journal of Pharmaceutical Sciences, 108, no. 3 (2019):1326-1333,
https://doi.org/10.1016/j.xphs.2018.10.054 . .

TY  - CONF
AU  - Milinković Budinčić, Jelena
AU  - Đekić, Ljiljana
AU  - Petrović, Lidija
AU  - Fraj, Jadranka
AU  - Ćirić, Ana
PY  - 2018
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5369
AB  - INTRODUCTION: The important current focus
in production of cosmetics is usage of vitamin E
(E), a natural antioxidant protective for tissues
from UV radiation, delays photoaging and provide
moisturizing effect. Encapsulation is needed
for its protection from high temperature, oxygen,
and light, during storage, and also for a potential
ability to control its release and delivery. Preparation
of microcapsules of desired characteristics
depends on various factors (size and nature of the
core substance, wall material, techniques and parameters
of encapsulation) [1, 2]. The study aimed
to evaluate chitosan/sodium lauryl ether sulfate
(Ch/SLES) microcapsules with E as a delivery system
for skin care.
MATERIALS AND METHODS: Microcapsules
were prepared by complex coacervation. Initially,
a 20% O/W emulsion with E (10% solution
in medium-chain triglycerides), stabilized with
the mixture of Ch (0.1 %) and SLES [3], was obtained
by Ultra Turrax T25 homogenization. The
emulsion, without or with a crosslinker, formaldehyde
(FA) or glutaraldehyde (GA), was spray
dried. The in vitro release profile of E from the microcapsule
samples (0.1 g) was studied in 100 g of
ethanol 80%, under continuous stirring at room
temperature. The dissolved E in supernatant aliquots
(2 ml) was analyzed during 90 min, by the
Halo DB-20S UV-VIS spectrophotometer.
RESULTS: The obtained release profiles were
analyzed by fi tt ing in different mathematical
models and in all samples correlate the best with
Korsmeyer-Peppas model. The diffusion exponent
n values (0.05-0.23) indicated non-Fickian
diffusion. We assumed that release of E was
based on a combination of rinsing from the surface
of the microcapsules [4] and diffusion
through the capsule wall. For microparticles with
GA, n was the lowest, the release was rapid and
the amount of release of the substance was higher
(i.e., more pronounced rinsing process), compared
with FA and microcapsules without the
crosslinker, where release of E was more controlled
by diffusion.
CONCLUSION: E vitamin release from Ch/
SLES microcapsules followed Korsmeyer-Peppas
kinetics. The selection of the crosslinker influenced
their surface properties, the surface amount and permeability of the capsule wall for E vitamine
diffusion.
PB  - Hungarian Society for Pharmaceutical Sciences
C3  - Acta Pharmaceutica Hungarica, 12th CESPT, Book of Abstracts
T1  - Chitosan/sodium lauryl ether sulfate microcapsules as carriers for vitamin E: in vitro release study
VL  - 88
IS  - 043
SP  - 173
EP  - 174
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5369
ER  - 

@conference{
author = "Milinković Budinčić, Jelena and Đekić, Ljiljana and Petrović, Lidija and Fraj, Jadranka and Ćirić, Ana",
year = "2018",
abstract = "INTRODUCTION: The important current focus
in production of cosmetics is usage of vitamin E
(E), a natural antioxidant protective for tissues
from UV radiation, delays photoaging and provide
moisturizing effect. Encapsulation is needed
for its protection from high temperature, oxygen,
and light, during storage, and also for a potential
ability to control its release and delivery. Preparation
of microcapsules of desired characteristics
depends on various factors (size and nature of the
core substance, wall material, techniques and parameters
of encapsulation) [1, 2]. The study aimed
to evaluate chitosan/sodium lauryl ether sulfate
(Ch/SLES) microcapsules with E as a delivery system
for skin care.
MATERIALS AND METHODS: Microcapsules
were prepared by complex coacervation. Initially,
a 20% O/W emulsion with E (10% solution
in medium-chain triglycerides), stabilized with
the mixture of Ch (0.1 %) and SLES [3], was obtained
by Ultra Turrax T25 homogenization. The
emulsion, without or with a crosslinker, formaldehyde
(FA) or glutaraldehyde (GA), was spray
dried. The in vitro release profile of E from the microcapsule
samples (0.1 g) was studied in 100 g of
ethanol 80%, under continuous stirring at room
temperature. The dissolved E in supernatant aliquots
(2 ml) was analyzed during 90 min, by the
Halo DB-20S UV-VIS spectrophotometer.
RESULTS: The obtained release profiles were
analyzed by fi tt ing in different mathematical
models and in all samples correlate the best with
Korsmeyer-Peppas model. The diffusion exponent
n values (0.05-0.23) indicated non-Fickian
diffusion. We assumed that release of E was
based on a combination of rinsing from the surface
of the microcapsules [4] and diffusion
through the capsule wall. For microparticles with
GA, n was the lowest, the release was rapid and
the amount of release of the substance was higher
(i.e., more pronounced rinsing process), compared
with FA and microcapsules without the
crosslinker, where release of E was more controlled
by diffusion.
CONCLUSION: E vitamin release from Ch/
SLES microcapsules followed Korsmeyer-Peppas
kinetics. The selection of the crosslinker influenced
their surface properties, the surface amount and permeability of the capsule wall for E vitamine
diffusion.",
publisher = "Hungarian Society for Pharmaceutical Sciences",
journal = "Acta Pharmaceutica Hungarica, 12th CESPT, Book of Abstracts",
title = "Chitosan/sodium lauryl ether sulfate microcapsules as carriers for vitamin E: in vitro release study",
volume = "88",
number = "043",
pages = "173-174",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5369"
}

Milinković Budinčić, J., Đekić, L., Petrović, L., Fraj, J.,& Ćirić, A.. (2018). Chitosan/sodium lauryl ether sulfate microcapsules as carriers for vitamin E: in vitro release study. in Acta Pharmaceutica Hungarica, 12th CESPT, Book of Abstracts
Hungarian Society for Pharmaceutical Sciences., 88(043), 173-174.
https://hdl.handle.net/21.15107/rcub_farfar_5369

Milinković Budinčić J, Đekić L, Petrović L, Fraj J, Ćirić A. Chitosan/sodium lauryl ether sulfate microcapsules as carriers for vitamin E: in vitro release study. in Acta Pharmaceutica Hungarica, 12th CESPT, Book of Abstracts. 2018;88(043):173-174.
https://hdl.handle.net/21.15107/rcub_farfar_5369 .

Milinković Budinčić, Jelena, Đekić, Ljiljana, Petrović, Lidija, Fraj, Jadranka, Ćirić, Ana, "Chitosan/sodium lauryl ether sulfate microcapsules as carriers for vitamin E: in vitro release study" in Acta Pharmaceutica Hungarica, 12th CESPT, Book of Abstracts, 88, no. 043 (2018):173-174,
https://hdl.handle.net/21.15107/rcub_farfar_5369 .

TY  - JOUR
AU  - Kalusević, Ana
AU  - Lević, Steva
AU  - Čalija, Bojan
AU  - Pantić, Milena
AU  - Belović, Miona
AU  - Pavlović, Vladimir
AU  - Bugarski, Branko
AU  - Milić, Jela
AU  - Žilić, Slađana
AU  - Nedović, Viktor
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3427
AB  - Black soybean coat is insufficiently valorised food production waste rich in anthocyanins. The goal of the study was to examine physicochemical properties of spray dried extract of black soybean coat in regard to carrier materials: maltodextrin, gum Arabic, and skimmed milk powder. Maltodextrin and gum Arabic-based microparticles were spherical and non-porous while skimmed milk powder-based were irregularly shaped. Low water activity of microparticles (0.31-0.33), good powders characteristics, high solubility (80.3-94.3%) and encapsulation yields (63.7-77.0%) were determined. All microparticles exhibited significant antioxidant capacity (243-386 mu molTE/g), good colour stability after three months of storage and antimicrobial activity. High content of total anthocyanins, with cyanidin-3-glucoside as predominant, were achieved. In vitro release of anthocyanins from microparticles was sustained, particularly from gum Arabic-based. These findings suggest that proposed simple eco-friendly extraction and microencapsulation procedures could serve as valuable tools for valorisation and conversion of black soybean coat into highly functional and stable food colourant.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Journal of Microencapsulation
T1  - Microencapsulation of anthocyanin-rich black soybean coat extract by spray drying using maltodextrin, gum Arabic and skimmed milk powder
VL  - 34
IS  - 5
SP  - 475
EP  - 487
DO  - 10.1080/02652048.2017.1354939
ER  - 

@article{
author = "Kalusević, Ana and Lević, Steva and Čalija, Bojan and Pantić, Milena and Belović, Miona and Pavlović, Vladimir and Bugarski, Branko and Milić, Jela and Žilić, Slađana and Nedović, Viktor",
year = "2017",
abstract = "Black soybean coat is insufficiently valorised food production waste rich in anthocyanins. The goal of the study was to examine physicochemical properties of spray dried extract of black soybean coat in regard to carrier materials: maltodextrin, gum Arabic, and skimmed milk powder. Maltodextrin and gum Arabic-based microparticles were spherical and non-porous while skimmed milk powder-based were irregularly shaped. Low water activity of microparticles (0.31-0.33), good powders characteristics, high solubility (80.3-94.3%) and encapsulation yields (63.7-77.0%) were determined. All microparticles exhibited significant antioxidant capacity (243-386 mu molTE/g), good colour stability after three months of storage and antimicrobial activity. High content of total anthocyanins, with cyanidin-3-glucoside as predominant, were achieved. In vitro release of anthocyanins from microparticles was sustained, particularly from gum Arabic-based. These findings suggest that proposed simple eco-friendly extraction and microencapsulation procedures could serve as valuable tools for valorisation and conversion of black soybean coat into highly functional and stable food colourant.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Journal of Microencapsulation",
title = "Microencapsulation of anthocyanin-rich black soybean coat extract by spray drying using maltodextrin, gum Arabic and skimmed milk powder",
volume = "34",
number = "5",
pages = "475-487",
doi = "10.1080/02652048.2017.1354939"
}

Kalusević, A., Lević, S., Čalija, B., Pantić, M., Belović, M., Pavlović, V., Bugarski, B., Milić, J., Žilić, S.,& Nedović, V.. (2017). Microencapsulation of anthocyanin-rich black soybean coat extract by spray drying using maltodextrin, gum Arabic and skimmed milk powder. in Journal of Microencapsulation
Taylor & Francis Ltd, Abingdon., 34(5), 475-487.
https://doi.org/10.1080/02652048.2017.1354939

Kalusević A, Lević S, Čalija B, Pantić M, Belović M, Pavlović V, Bugarski B, Milić J, Žilić S, Nedović V. Microencapsulation of anthocyanin-rich black soybean coat extract by spray drying using maltodextrin, gum Arabic and skimmed milk powder. in Journal of Microencapsulation. 2017;34(5):475-487.
doi:10.1080/02652048.2017.1354939 .

Kalusević, Ana, Lević, Steva, Čalija, Bojan, Pantić, Milena, Belović, Miona, Pavlović, Vladimir, Bugarski, Branko, Milić, Jela, Žilić, Slađana, Nedović, Viktor, "Microencapsulation of anthocyanin-rich black soybean coat extract by spray drying using maltodextrin, gum Arabic and skimmed milk powder" in Journal of Microencapsulation, 34, no. 5 (2017):475-487,
https://doi.org/10.1080/02652048.2017.1354939 . .

TY  - JOUR
AU  - Kalusević, Ana
AU  - Lević, Steva
AU  - Čalija, Bojan
AU  - Milić, Jela
AU  - Pavlović, Vladimir
AU  - Bugarski, Branko
AU  - Nedović, Viktor
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2876
AB  - The goal of this study was to investigate the characteristics of grape skin extract (GSE) spray dried with different carriers: maltodextrin (MD), gum Arabic (GA) and skim milk powder (SMP). The grape skin extract was obtained from winery by-product of red grape variety Prokupac (Vitis vinifera L.). The morphology of the powders, their thermal, chemical and physical properties (water activity, bulk and tapped densities, solubility), as well as release studies in different pH conditions were analyzed. Total anthocyanin content and total phenolic content were determined by spectrophotometric methods. MD and GA-based microparticles were non-porous and spherical, while SMP-based ones were irregularly shaped. The process of spray drying Prokupac GSE using these three carriers produced powders with low water activity (0.24-0.28), good powder characteristics, high yields, and solubility higher than 90%. The obtained dissolution/release profiles indicated prolonged release of anthocyanins and phenolic compounds in different mediums, especially from GSE/GA microparticles. These results have shown that grape skin as the main by-product of wine production could be used as a source of natural colorants and bioactive compounds, and microencapsulation as a promising technique for the protection of these compounds, their stabilization in longer periods and prolonged release.
PB  - Springer India, New Delhi
T2  - Journal of Food Science and Technology-Mysore
T1  - Effects of different carrier materials on physicochemical properties of microencapsulated grape skin extract
VL  - 54
IS  - 11
SP  - 3411
EP  - 3420
DO  - 10.1007/s13197-017-2790-6
ER  - 

@article{
author = "Kalusević, Ana and Lević, Steva and Čalija, Bojan and Milić, Jela and Pavlović, Vladimir and Bugarski, Branko and Nedović, Viktor",
year = "2017",
abstract = "The goal of this study was to investigate the characteristics of grape skin extract (GSE) spray dried with different carriers: maltodextrin (MD), gum Arabic (GA) and skim milk powder (SMP). The grape skin extract was obtained from winery by-product of red grape variety Prokupac (Vitis vinifera L.). The morphology of the powders, their thermal, chemical and physical properties (water activity, bulk and tapped densities, solubility), as well as release studies in different pH conditions were analyzed. Total anthocyanin content and total phenolic content were determined by spectrophotometric methods. MD and GA-based microparticles were non-porous and spherical, while SMP-based ones were irregularly shaped. The process of spray drying Prokupac GSE using these three carriers produced powders with low water activity (0.24-0.28), good powder characteristics, high yields, and solubility higher than 90%. The obtained dissolution/release profiles indicated prolonged release of anthocyanins and phenolic compounds in different mediums, especially from GSE/GA microparticles. These results have shown that grape skin as the main by-product of wine production could be used as a source of natural colorants and bioactive compounds, and microencapsulation as a promising technique for the protection of these compounds, their stabilization in longer periods and prolonged release.",
publisher = "Springer India, New Delhi",
journal = "Journal of Food Science and Technology-Mysore",
title = "Effects of different carrier materials on physicochemical properties of microencapsulated grape skin extract",
volume = "54",
number = "11",
pages = "3411-3420",
doi = "10.1007/s13197-017-2790-6"
}

Kalusević, A., Lević, S., Čalija, B., Milić, J., Pavlović, V., Bugarski, B.,& Nedović, V.. (2017). Effects of different carrier materials on physicochemical properties of microencapsulated grape skin extract. in Journal of Food Science and Technology-Mysore
Springer India, New Delhi., 54(11), 3411-3420.
https://doi.org/10.1007/s13197-017-2790-6

Kalusević A, Lević S, Čalija B, Milić J, Pavlović V, Bugarski B, Nedović V. Effects of different carrier materials on physicochemical properties of microencapsulated grape skin extract. in Journal of Food Science and Technology-Mysore. 2017;54(11):3411-3420.
doi:10.1007/s13197-017-2790-6 .

Kalusević, Ana, Lević, Steva, Čalija, Bojan, Milić, Jela, Pavlović, Vladimir, Bugarski, Branko, Nedović, Viktor, "Effects of different carrier materials on physicochemical properties of microencapsulated grape skin extract" in Journal of Food Science and Technology-Mysore, 54, no. 11 (2017):3411-3420,
https://doi.org/10.1007/s13197-017-2790-6 . .

TY  - JOUR
AU  - Kalusević, Ana
AU  - Lević, Steva
AU  - Čalija, Bojan
AU  - Pantić, Milena
AU  - Belović, Miona
AU  - Pavlović, Vladimir
AU  - Bugarski, Branko
AU  - Milić, Jela
AU  - Žilić, Slađana
AU  - Nedović, Viktor
PY  - 2017
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2797
AB  - Black soybean coat is insufficiently valorised food production waste rich in anthocyanins. The goal of the study was to examine physicochemical properties of spray dried extract of black soybean coat in regard to carrier materials: maltodextrin, gum Arabic, and skimmed milk powder. Maltodextrin and gum Arabic-based microparticles were spherical and non-porous while skimmed milk powder-based were irregularly shaped. Low water activity of microparticles (0.31-0.33), good powders characteristics, high solubility (80.3-94.3%) and encapsulation yields (63.7-77.0%) were determined. All microparticles exhibited significant antioxidant capacity (243-386 mu molTE/g), good colour stability after three months of storage and antimicrobial activity. High content of total anthocyanins, with cyanidin-3-glucoside as predominant, were achieved. In vitro release of anthocyanins from microparticles was sustained, particularly from gum Arabic-based. These findings suggest that proposed simple eco-friendly extraction and microencapsulation procedures could serve as valuable tools for valorisation and conversion of black soybean coat into highly functional and stable food colourant.
PB  - Taylor & Francis Ltd, Abingdon
T2  - Journal of Microencapsulation
T1  - Microencapsulation of anthocyanin-rich black soybean coat extract by spray drying using maltodextrin, gum Arabic and skimmed milk powder
VL  - 34
IS  - 5
SP  - 475
EP  - 487
DO  - 10.1080/02652048.2017.1354939
ER  - 

@article{
author = "Kalusević, Ana and Lević, Steva and Čalija, Bojan and Pantić, Milena and Belović, Miona and Pavlović, Vladimir and Bugarski, Branko and Milić, Jela and Žilić, Slađana and Nedović, Viktor",
year = "2017",
abstract = "Black soybean coat is insufficiently valorised food production waste rich in anthocyanins. The goal of the study was to examine physicochemical properties of spray dried extract of black soybean coat in regard to carrier materials: maltodextrin, gum Arabic, and skimmed milk powder. Maltodextrin and gum Arabic-based microparticles were spherical and non-porous while skimmed milk powder-based were irregularly shaped. Low water activity of microparticles (0.31-0.33), good powders characteristics, high solubility (80.3-94.3%) and encapsulation yields (63.7-77.0%) were determined. All microparticles exhibited significant antioxidant capacity (243-386 mu molTE/g), good colour stability after three months of storage and antimicrobial activity. High content of total anthocyanins, with cyanidin-3-glucoside as predominant, were achieved. In vitro release of anthocyanins from microparticles was sustained, particularly from gum Arabic-based. These findings suggest that proposed simple eco-friendly extraction and microencapsulation procedures could serve as valuable tools for valorisation and conversion of black soybean coat into highly functional and stable food colourant.",
publisher = "Taylor & Francis Ltd, Abingdon",
journal = "Journal of Microencapsulation",
title = "Microencapsulation of anthocyanin-rich black soybean coat extract by spray drying using maltodextrin, gum Arabic and skimmed milk powder",
volume = "34",
number = "5",
pages = "475-487",
doi = "10.1080/02652048.2017.1354939"
}

Kalusević, A., Lević, S., Čalija, B., Pantić, M., Belović, M., Pavlović, V., Bugarski, B., Milić, J., Žilić, S.,& Nedović, V.. (2017). Microencapsulation of anthocyanin-rich black soybean coat extract by spray drying using maltodextrin, gum Arabic and skimmed milk powder. in Journal of Microencapsulation
Taylor & Francis Ltd, Abingdon., 34(5), 475-487.
https://doi.org/10.1080/02652048.2017.1354939

Kalusević A, Lević S, Čalija B, Pantić M, Belović M, Pavlović V, Bugarski B, Milić J, Žilić S, Nedović V. Microencapsulation of anthocyanin-rich black soybean coat extract by spray drying using maltodextrin, gum Arabic and skimmed milk powder. in Journal of Microencapsulation. 2017;34(5):475-487.
doi:10.1080/02652048.2017.1354939 .

Kalusević, Ana, Lević, Steva, Čalija, Bojan, Pantić, Milena, Belović, Miona, Pavlović, Vladimir, Bugarski, Branko, Milić, Jela, Žilić, Slađana, Nedović, Viktor, "Microencapsulation of anthocyanin-rich black soybean coat extract by spray drying using maltodextrin, gum Arabic and skimmed milk powder" in Journal of Microencapsulation, 34, no. 5 (2017):475-487,
https://doi.org/10.1080/02652048.2017.1354939 . .

TY  - JOUR
AU  - Đekić, Ljiljana
AU  - Martinović, Martina
AU  - Stepanović-Petrović, Radica
AU  - Micov, Ana
AU  - Tomić, Maja
AU  - Primorac, Marija
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2714
AB  - The study investigated usage of hydrogel of an anionic polymer xanthan gum for design of ibuprofen-loaded hydrogel-thickened microemulsions (HTMs) from the nonionic oil-in-water microemulsion (M). Xanthan gum demonstrated the performances of a thickening agent in physically stable HTMs at 5 +/- 3 degrees C, 20 +/- 3 degrees C, and 40 +/- 1 degrees C during 6 months. The results of physicochemical characterization (pH, conductivity, rheological behaviour, spreadability) indicated that HTMs containing 0.25-1.00% of the polymer had colloidal structure with oil nanodroplets of 1434 +/- 0.98 nm (PdI 0220 +/- 0.075) dispersed in aqueous phase thickened with the polymer gel network which strength depended on the polymer concentration. HTMs with ibuprofen (5%) were evaluated as percutaneous drug delivery carriers. In vitro ibuprofen release from HTMs followed zero order kinetic (r > 0.995) for 12 h, while the referent hydrogel was described by Higuchi model. The HTM with optimized drug release rate and spreadability (HTM1) and the polymer-free microemulsion (M) were assessed and compared with the referent hydrogel in in vivo studies in rats. HTM1 and M were significantly more efficacious than reference hydrogel in producing antihyperalgesic and at lower extent antiedematous activity in prophylactic topical treatment protocol, whilst they were comparable in producing antihyperalgesic/antiedematous effects in therapeutic protocol. Topical treatments produced no obvious skin irritation.
PB  - Elsevier Science BV, Amsterdam
T2  - European Journal of Pharmaceutical Sciences
T1  - Formulation of hydrogel-thickened nonionic microemulsions with enhanced percutaneous delivery of ibuprofen assessed in vivo in rats
VL  - 92
SP  - 255
EP  - 265
DO  - 10.1016/j.ejps.2016.05.005
ER  - 

@article{
author = "Đekić, Ljiljana and Martinović, Martina and Stepanović-Petrović, Radica and Micov, Ana and Tomić, Maja and Primorac, Marija",
year = "2016",
abstract = "The study investigated usage of hydrogel of an anionic polymer xanthan gum for design of ibuprofen-loaded hydrogel-thickened microemulsions (HTMs) from the nonionic oil-in-water microemulsion (M). Xanthan gum demonstrated the performances of a thickening agent in physically stable HTMs at 5 +/- 3 degrees C, 20 +/- 3 degrees C, and 40 +/- 1 degrees C during 6 months. The results of physicochemical characterization (pH, conductivity, rheological behaviour, spreadability) indicated that HTMs containing 0.25-1.00% of the polymer had colloidal structure with oil nanodroplets of 1434 +/- 0.98 nm (PdI 0220 +/- 0.075) dispersed in aqueous phase thickened with the polymer gel network which strength depended on the polymer concentration. HTMs with ibuprofen (5%) were evaluated as percutaneous drug delivery carriers. In vitro ibuprofen release from HTMs followed zero order kinetic (r > 0.995) for 12 h, while the referent hydrogel was described by Higuchi model. The HTM with optimized drug release rate and spreadability (HTM1) and the polymer-free microemulsion (M) were assessed and compared with the referent hydrogel in in vivo studies in rats. HTM1 and M were significantly more efficacious than reference hydrogel in producing antihyperalgesic and at lower extent antiedematous activity in prophylactic topical treatment protocol, whilst they were comparable in producing antihyperalgesic/antiedematous effects in therapeutic protocol. Topical treatments produced no obvious skin irritation.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "European Journal of Pharmaceutical Sciences",
title = "Formulation of hydrogel-thickened nonionic microemulsions with enhanced percutaneous delivery of ibuprofen assessed in vivo in rats",
volume = "92",
pages = "255-265",
doi = "10.1016/j.ejps.2016.05.005"
}

Đekić, L., Martinović, M., Stepanović-Petrović, R., Micov, A., Tomić, M.,& Primorac, M.. (2016). Formulation of hydrogel-thickened nonionic microemulsions with enhanced percutaneous delivery of ibuprofen assessed in vivo in rats. in European Journal of Pharmaceutical Sciences
Elsevier Science BV, Amsterdam., 92, 255-265.
https://doi.org/10.1016/j.ejps.2016.05.005

Đekić L, Martinović M, Stepanović-Petrović R, Micov A, Tomić M, Primorac M. Formulation of hydrogel-thickened nonionic microemulsions with enhanced percutaneous delivery of ibuprofen assessed in vivo in rats. in European Journal of Pharmaceutical Sciences. 2016;92:255-265.
doi:10.1016/j.ejps.2016.05.005 .

Đekić, Ljiljana, Martinović, Martina, Stepanović-Petrović, Radica, Micov, Ana, Tomić, Maja, Primorac, Marija, "Formulation of hydrogel-thickened nonionic microemulsions with enhanced percutaneous delivery of ibuprofen assessed in vivo in rats" in European Journal of Pharmaceutical Sciences, 92 (2016):255-265,
https://doi.org/10.1016/j.ejps.2016.05.005 . .

TY  - JOUR
AU  - Milašinović, Nikola
AU  - Čalija, Bojan
AU  - Vidović, Bojana
AU  - Crevar-Sakač, Milkica
AU  - Vujić, Zorica
AU  - Knežević-Jugović, Zorica
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2607
AB  - The aim of this study was to investigate the encapsulation of a-lipoic acid (LA) onto chitosan microbeads in order to achieve a sustained release. Microbeads of different compositions were synthetized by inverse emulsion method, and characterized by in vitro swelling and drug release studies, FT-IR, DSC and SEM analyses. The encapsulation of LA was performed by swelling of previously synthetized dry microbeads in LA solution. The encapsulation efficiency was determined in terms of LA concentration, using liquid chromatography tandem mass spectrometry (LC-MS/MS). The swelling of synthetized microbeads was well pronounced in the acidic media. FT-IR analysis confirmed the presence of LA in microbeads and its interactions with matrices, which was in agreement with the results revealed by DSC analysis. SEM analysis revealed the porous structure, micron-size and spherical shape of the synthetized beads. Encapsulation efficiency was in the range from 46.8 to 58.5% depending on the hydrogel composition. The microbeads exhibited sustained release of LA under conditions mimicking gastrointestinal tract. Results of antioxidant activity of released LA showed good correlation with the concentration of the released LA. The obtained results suggested that synthetized microbeads have promising potential for oral administration with reduced dosing frequency in comparison to the immediate release formulations.
PB  - Elsevier Science BV, Amsterdam
T2  - Journal of the Taiwan Institute of Chemical Engineers
T1  - Sustained release of alpha-lipoic acid from chitosan microbeads synthetized by inverse emulsion method
VL  - 60
SP  - 106
EP  - 112
DO  - 10.1016/j.jtice.2015.10.037
ER  - 

@article{
author = "Milašinović, Nikola and Čalija, Bojan and Vidović, Bojana and Crevar-Sakač, Milkica and Vujić, Zorica and Knežević-Jugović, Zorica",
year = "2016",
abstract = "The aim of this study was to investigate the encapsulation of a-lipoic acid (LA) onto chitosan microbeads in order to achieve a sustained release. Microbeads of different compositions were synthetized by inverse emulsion method, and characterized by in vitro swelling and drug release studies, FT-IR, DSC and SEM analyses. The encapsulation of LA was performed by swelling of previously synthetized dry microbeads in LA solution. The encapsulation efficiency was determined in terms of LA concentration, using liquid chromatography tandem mass spectrometry (LC-MS/MS). The swelling of synthetized microbeads was well pronounced in the acidic media. FT-IR analysis confirmed the presence of LA in microbeads and its interactions with matrices, which was in agreement with the results revealed by DSC analysis. SEM analysis revealed the porous structure, micron-size and spherical shape of the synthetized beads. Encapsulation efficiency was in the range from 46.8 to 58.5% depending on the hydrogel composition. The microbeads exhibited sustained release of LA under conditions mimicking gastrointestinal tract. Results of antioxidant activity of released LA showed good correlation with the concentration of the released LA. The obtained results suggested that synthetized microbeads have promising potential for oral administration with reduced dosing frequency in comparison to the immediate release formulations.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Journal of the Taiwan Institute of Chemical Engineers",
title = "Sustained release of alpha-lipoic acid from chitosan microbeads synthetized by inverse emulsion method",
volume = "60",
pages = "106-112",
doi = "10.1016/j.jtice.2015.10.037"
}

Milašinović, N., Čalija, B., Vidović, B., Crevar-Sakač, M., Vujić, Z.,& Knežević-Jugović, Z.. (2016). Sustained release of alpha-lipoic acid from chitosan microbeads synthetized by inverse emulsion method. in Journal of the Taiwan Institute of Chemical Engineers
Elsevier Science BV, Amsterdam., 60, 106-112.
https://doi.org/10.1016/j.jtice.2015.10.037

Milašinović N, Čalija B, Vidović B, Crevar-Sakač M, Vujić Z, Knežević-Jugović Z. Sustained release of alpha-lipoic acid from chitosan microbeads synthetized by inverse emulsion method. in Journal of the Taiwan Institute of Chemical Engineers. 2016;60:106-112.
doi:10.1016/j.jtice.2015.10.037 .

Milašinović, Nikola, Čalija, Bojan, Vidović, Bojana, Crevar-Sakač, Milkica, Vujić, Zorica, Knežević-Jugović, Zorica, "Sustained release of alpha-lipoic acid from chitosan microbeads synthetized by inverse emulsion method" in Journal of the Taiwan Institute of Chemical Engineers, 60 (2016):106-112,
https://doi.org/10.1016/j.jtice.2015.10.037 . .

TY  - JOUR
AU  - Vidović, Bojana
AU  - Milašinović, Nikola
AU  - Kotur-Stevuljević, Jelena
AU  - Dilber, Sanda
AU  - Kalagasidis-Krusić, Melina T.
AU  - Đorđević, Brižita
AU  - Knežević-Jugović, Zorica
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2605
AB  - Alpha-lipoic acid is an organosulphur compound well-known for its therapeutic potential and antioxidant properties. However, the effective use of alpha-lipoic acid depends on biological plasma half-life and its preserving stability, which could be improved by encapsulation. In this study, alpha-lipoic acid was incorporated into chitosan microparticles obtained by reverse emulsion crosslinking technique, as well as into microparticles of alginate/gelatin crosslinked with zinc ions. Encapsulation of alpha-lipoic acid in both cases was carried out by swelling of synthesized dried microparticles by their dipping in a solution of the active substance under strictly controlled conditions. Encapsulation efficiency of alpha-lipoic acid obtained in this study was up to 53.9%. The structural interaction of alpha-lipoic acid with the carriers was revealed by Fourier transform infrared spectroscopy. In vitro released studies showed that controlled release of alpha-lipoic acid was achieved through its encapsulation into chitosan microparticles. The results of in vitro antioxidative activity assays of released alpha-lipoic acid indicated that antioxidant activity was preserved at a satisfactory level. These obtained results suggested that chitosan microparticles could be suitable for modeling the controlled release of alpha-lipoic acid.
PB  - Savez hemijskih inženjera, Beograd
T2  - Hemijska industrija
T1  - Encapsulation of alpha-lipoic acid into chitosan and alginate/gelatin hydrogel microparticles and its in vitro antioxidant activity
VL  - 70
IS  - 1
SP  - 49
EP  - 58
DO  - 10.2298/HEMIND141119010V
ER  - 

@article{
author = "Vidović, Bojana and Milašinović, Nikola and Kotur-Stevuljević, Jelena and Dilber, Sanda and Kalagasidis-Krusić, Melina T. and Đorđević, Brižita and Knežević-Jugović, Zorica",
year = "2016",
abstract = "Alpha-lipoic acid is an organosulphur compound well-known for its therapeutic potential and antioxidant properties. However, the effective use of alpha-lipoic acid depends on biological plasma half-life and its preserving stability, which could be improved by encapsulation. In this study, alpha-lipoic acid was incorporated into chitosan microparticles obtained by reverse emulsion crosslinking technique, as well as into microparticles of alginate/gelatin crosslinked with zinc ions. Encapsulation of alpha-lipoic acid in both cases was carried out by swelling of synthesized dried microparticles by their dipping in a solution of the active substance under strictly controlled conditions. Encapsulation efficiency of alpha-lipoic acid obtained in this study was up to 53.9%. The structural interaction of alpha-lipoic acid with the carriers was revealed by Fourier transform infrared spectroscopy. In vitro released studies showed that controlled release of alpha-lipoic acid was achieved through its encapsulation into chitosan microparticles. The results of in vitro antioxidative activity assays of released alpha-lipoic acid indicated that antioxidant activity was preserved at a satisfactory level. These obtained results suggested that chitosan microparticles could be suitable for modeling the controlled release of alpha-lipoic acid.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Hemijska industrija",
title = "Encapsulation of alpha-lipoic acid into chitosan and alginate/gelatin hydrogel microparticles and its in vitro antioxidant activity",
volume = "70",
number = "1",
pages = "49-58",
doi = "10.2298/HEMIND141119010V"
}

Vidović, B., Milašinović, N., Kotur-Stevuljević, J., Dilber, S., Kalagasidis-Krusić, M. T., Đorđević, B.,& Knežević-Jugović, Z.. (2016). Encapsulation of alpha-lipoic acid into chitosan and alginate/gelatin hydrogel microparticles and its in vitro antioxidant activity. in Hemijska industrija
Savez hemijskih inženjera, Beograd., 70(1), 49-58.
https://doi.org/10.2298/HEMIND141119010V

Vidović B, Milašinović N, Kotur-Stevuljević J, Dilber S, Kalagasidis-Krusić MT, Đorđević B, Knežević-Jugović Z. Encapsulation of alpha-lipoic acid into chitosan and alginate/gelatin hydrogel microparticles and its in vitro antioxidant activity. in Hemijska industrija. 2016;70(1):49-58.
doi:10.2298/HEMIND141119010V .

Vidović, Bojana, Milašinović, Nikola, Kotur-Stevuljević, Jelena, Dilber, Sanda, Kalagasidis-Krusić, Melina T., Đorđević, Brižita, Knežević-Jugović, Zorica, "Encapsulation of alpha-lipoic acid into chitosan and alginate/gelatin hydrogel microparticles and its in vitro antioxidant activity" in Hemijska industrija, 70, no. 1 (2016):49-58,
https://doi.org/10.2298/HEMIND141119010V . .

TY  - JOUR
AU  - Đekić, Ljiljana
AU  - Krajišnik, Danina
AU  - Micić, Zorica
AU  - Čalija, Bojan
PY  - 2016
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2762
AB  - Carbomer based hydrogels with 1% of 18 beta-glycyrrhetinic acid complex in the form of phytosomes (18 beta-GAP) were formulated by using Carbopol (R) 980 and Carbopol (R) Ultrez 10 as gelling agents (1%), sodium hydroxide (neutralizing agent) (0.4%), glycerol (humectant) (10%), Sepicide (R) HB (preservative) (1%), and water (up to 100%). In order to prepare the hydrogels, 18 beta-GAP was dispersed into the water phase prior the carbomer neutralization or the 18 beta-GAP aqueous dispersion was added into the previously neutralized carbomer dispersion. The pH values (6.71 +/- 0.16-7.09 +/- 0.06), soft semisolid consistency and spreadability (25.7 +/- 1.5 mm-33.7 +/- 0.6 mm) of the hydrogels were acceptable for application on the skin. The influence of the preparation procedure, the carbomer type, and addition of a humectant (glycerol 10%) on physicochemical properties was evaluated using light microscopy, DSC analysis and rheological behavior characterization. The preparation procedure, the carbomer type, and the addition of the humectant did not affect significantly organoleptic and morphological characteristics and spread-ability of the hydrogels. The Carbopol (R) 980 based hydrogels were more sensitive than hydrogels based on Carbopol (R) Ultrez 10 upon thermal variations regarding the water evaporation loss. Physical stability of the investigated hydrogels was satisfactory for minimum 30 days of storage at a temperature range from +4 degrees C up to +40 degrees C.
PB  - Elsevier Science BV, Amsterdam
T2  - Journal of Drug Delivery Science and Technology
T1  - Formulation and physicochemical characterization of hydrogels with 18 beta-glycyrrhetinic acid/phospholipid complex phytosomes
VL  - 35
SP  - 81
EP  - 90
DO  - 10.1016/j.jddst.2016.06.008
ER  - 

@article{
author = "Đekić, Ljiljana and Krajišnik, Danina and Micić, Zorica and Čalija, Bojan",
year = "2016",
abstract = "Carbomer based hydrogels with 1% of 18 beta-glycyrrhetinic acid complex in the form of phytosomes (18 beta-GAP) were formulated by using Carbopol (R) 980 and Carbopol (R) Ultrez 10 as gelling agents (1%), sodium hydroxide (neutralizing agent) (0.4%), glycerol (humectant) (10%), Sepicide (R) HB (preservative) (1%), and water (up to 100%). In order to prepare the hydrogels, 18 beta-GAP was dispersed into the water phase prior the carbomer neutralization or the 18 beta-GAP aqueous dispersion was added into the previously neutralized carbomer dispersion. The pH values (6.71 +/- 0.16-7.09 +/- 0.06), soft semisolid consistency and spreadability (25.7 +/- 1.5 mm-33.7 +/- 0.6 mm) of the hydrogels were acceptable for application on the skin. The influence of the preparation procedure, the carbomer type, and addition of a humectant (glycerol 10%) on physicochemical properties was evaluated using light microscopy, DSC analysis and rheological behavior characterization. The preparation procedure, the carbomer type, and the addition of the humectant did not affect significantly organoleptic and morphological characteristics and spread-ability of the hydrogels. The Carbopol (R) 980 based hydrogels were more sensitive than hydrogels based on Carbopol (R) Ultrez 10 upon thermal variations regarding the water evaporation loss. Physical stability of the investigated hydrogels was satisfactory for minimum 30 days of storage at a temperature range from +4 degrees C up to +40 degrees C.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "Journal of Drug Delivery Science and Technology",
title = "Formulation and physicochemical characterization of hydrogels with 18 beta-glycyrrhetinic acid/phospholipid complex phytosomes",
volume = "35",
pages = "81-90",
doi = "10.1016/j.jddst.2016.06.008"
}

Đekić, L., Krajišnik, D., Micić, Z.,& Čalija, B.. (2016). Formulation and physicochemical characterization of hydrogels with 18 beta-glycyrrhetinic acid/phospholipid complex phytosomes. in Journal of Drug Delivery Science and Technology
Elsevier Science BV, Amsterdam., 35, 81-90.
https://doi.org/10.1016/j.jddst.2016.06.008

Đekić L, Krajišnik D, Micić Z, Čalija B. Formulation and physicochemical characterization of hydrogels with 18 beta-glycyrrhetinic acid/phospholipid complex phytosomes. in Journal of Drug Delivery Science and Technology. 2016;35:81-90.
doi:10.1016/j.jddst.2016.06.008 .

Đekić, Ljiljana, Krajišnik, Danina, Micić, Zorica, Čalija, Bojan, "Formulation and physicochemical characterization of hydrogels with 18 beta-glycyrrhetinic acid/phospholipid complex phytosomes" in Journal of Drug Delivery Science and Technology, 35 (2016):81-90,
https://doi.org/10.1016/j.jddst.2016.06.008 . .

TY  - JOUR
AU  - Đekić, Ljiljana
AU  - Krajišnik, Danina
AU  - Martinović, Martina
AU  - Đorđević, Dragana
AU  - Primorac, Marija
PY  - 2015
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2468
AB  - Suitability of liquid lecithin (i.e., solution of lecithin in soy bean oil with similar to 60% w/w of phospholipids) for formation of gels, upon addition of water solution of poloxamer 407, was investigated, and formulated systems were evaluated as carriers for percutaneous delivery of ibuprofen. Formulation study of pseudoternary system liquid lecithin/poloxamer 407/water at constant liquid lecithin/poloxamer 407 mass ratio (2.0) revealed that minimum concentrations of liquid lecithin and poloxamer 407 required for formation of gel like systems were 15.75% w/w and 13.13% w/w, respectively, while the maximum content of water was 60.62% w/w. The systems comprising water concentrations in a range from 55 to 60.62% w/w were soft semisolids suitable for topical application, and they were selected for physicochemical and biopharmaceutical evaluation. Analysis of conductivity results and light microscopy examination revealed that investigated systems were water dilutable dispersions of spherical oligolamellar associates of phospholipids and triglyceride molecules in the copolymer water solution. Rheological behavior evaluation results indicated that the investigated gels were thermosensitive shear thinning systems. Ibuprofen (5% w/w) was incorporated by dispersing into the previously prepared carriers. Drug-loaded systems were physically stable at storage temperature from 5 +/- 3 degrees C to 40 +/- 2 degrees C, for 30 days. In vitro ibuprofen release was in accordance with the Higuchi model (r(H) > 0.95) and sustained for 12 h. The obtained results implicated that formulated LLPBGs, optimized regarding drug release and organoleptic properties, represent promising carriers for sustained percutaneous drug delivery of poorly soluble drugs.
PB  - Elsevier Science BV, Amsterdam
T2  - International Journal of Pharmaceutics
T1  - Characterization of gelation process and drug release profile of thermosensitive liquid lecithin/poloxamer 407 based gels as carriers for percutaneous delivery of ibuprofen
VL  - 490
IS  - 1-2
SP  - 180
EP  - 189
DO  - 10.1016/j.ijpharm.2015.05.040
ER  - 

@article{
author = "Đekić, Ljiljana and Krajišnik, Danina and Martinović, Martina and Đorđević, Dragana and Primorac, Marija",
year = "2015",
abstract = "Suitability of liquid lecithin (i.e., solution of lecithin in soy bean oil with similar to 60% w/w of phospholipids) for formation of gels, upon addition of water solution of poloxamer 407, was investigated, and formulated systems were evaluated as carriers for percutaneous delivery of ibuprofen. Formulation study of pseudoternary system liquid lecithin/poloxamer 407/water at constant liquid lecithin/poloxamer 407 mass ratio (2.0) revealed that minimum concentrations of liquid lecithin and poloxamer 407 required for formation of gel like systems were 15.75% w/w and 13.13% w/w, respectively, while the maximum content of water was 60.62% w/w. The systems comprising water concentrations in a range from 55 to 60.62% w/w were soft semisolids suitable for topical application, and they were selected for physicochemical and biopharmaceutical evaluation. Analysis of conductivity results and light microscopy examination revealed that investigated systems were water dilutable dispersions of spherical oligolamellar associates of phospholipids and triglyceride molecules in the copolymer water solution. Rheological behavior evaluation results indicated that the investigated gels were thermosensitive shear thinning systems. Ibuprofen (5% w/w) was incorporated by dispersing into the previously prepared carriers. Drug-loaded systems were physically stable at storage temperature from 5 +/- 3 degrees C to 40 +/- 2 degrees C, for 30 days. In vitro ibuprofen release was in accordance with the Higuchi model (r(H) > 0.95) and sustained for 12 h. The obtained results implicated that formulated LLPBGs, optimized regarding drug release and organoleptic properties, represent promising carriers for sustained percutaneous drug delivery of poorly soluble drugs.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "International Journal of Pharmaceutics",
title = "Characterization of gelation process and drug release profile of thermosensitive liquid lecithin/poloxamer 407 based gels as carriers for percutaneous delivery of ibuprofen",
volume = "490",
number = "1-2",
pages = "180-189",
doi = "10.1016/j.ijpharm.2015.05.040"
}

Đekić, L., Krajišnik, D., Martinović, M., Đorđević, D.,& Primorac, M.. (2015). Characterization of gelation process and drug release profile of thermosensitive liquid lecithin/poloxamer 407 based gels as carriers for percutaneous delivery of ibuprofen. in International Journal of Pharmaceutics
Elsevier Science BV, Amsterdam., 490(1-2), 180-189.
https://doi.org/10.1016/j.ijpharm.2015.05.040

Đekić L, Krajišnik D, Martinović M, Đorđević D, Primorac M. Characterization of gelation process and drug release profile of thermosensitive liquid lecithin/poloxamer 407 based gels as carriers for percutaneous delivery of ibuprofen. in International Journal of Pharmaceutics. 2015;490(1-2):180-189.
doi:10.1016/j.ijpharm.2015.05.040 .

Đekić, Ljiljana, Krajišnik, Danina, Martinović, Martina, Đorđević, Dragana, Primorac, Marija, "Characterization of gelation process and drug release profile of thermosensitive liquid lecithin/poloxamer 407 based gels as carriers for percutaneous delivery of ibuprofen" in International Journal of Pharmaceutics, 490, no. 1-2 (2015):180-189,
https://doi.org/10.1016/j.ijpharm.2015.05.040 . .

TY  - JOUR
AU  - Đekić, Ljiljana
AU  - Primorac, Marija
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2286
AB  - Development of self-dispersing drug delivery systems (SMEDDS) is a modern strategy for oral delivery improvement of poorly soluble drugs. Self-microemulsifying drug delivery systems (SMEDDS) are isotropic mixtures of oils and hydrophilic surfactants, which form oil-in-water (o/w) microemulsions by dilution in aqueous media (e.g., gastrointestinal fluids). Formulation of SMEDDS carriers requires consideration of a large number of formulation parameters and their influences on process of self-microemulsifying and releasing of drug. The aim of this work was formulation and characterization of SMEDDS for oral administration of ibuprofen. In the experimental work, two series of potential SMEDDS were prepared (M1-M10), using surfactant (Labrasol®, Gattefosse), cosurfactant (PEG­40 hydrogenated castor (Cremophor® RH40), and oil (medium chain triglycerides (Crodamol® GTCC) and olive oil (Cropur® Olive)), at surfactant-to-cosurfactant mass ratios (Km) 9:1, 7:3, 5:5, 3:7, and 1:9, and 10 or 20% of the oil phase. Ibuprofen was dissolved in formulations in concentration of 10%. Characterization of the investigated formulations included evaluation of physical stability, self-microemulsification ability in 0.1 M HCl (pH 1.2) and phosphate buffer pH 7.2 (USP) and in vitro drug release. Formation of o/w microemulsions with the average droplet size (Z-ave) up to 100 nm, was observed in dispersions of formulations prepared with 10 mass% of medium chain triglycerides, within the entire investigated range of the Km values (M1-M5). These formulations were selected as SMEDDS. Results of characterization pointed out the importance of the type and concentration of the oil as well as the Km value for the self-microemulsing ability, as well as drug release kinetics from the investigated SMEDDS. Ibuprofen release was in accordance with the request of USP 30-NF 25 (at least 80% after 60 min) from the formulations M1 (Km 9:1) and M5 (Km 1:9). Furthermore, the ibuprofen release was completed after 10 min from formulation M1, while the release from the carrier M5 (~30%) as well as from the commercial tablets Brufen® (~55%) and soft capsules Rapidol® (~65%), examined under the same conditions, was significantly slower. The present study revealed that the formulation M1 represents a potential SMEDDS which efficiently dissolves ibuprofen in acidic media, with potential to minimize the side effects, while on introduction into alkaline intestinal environment, the drug may rapidly release from the carrier and undergo absorption.
AB  - Razvoj samo-mikroemulgujućih nosača je značajna savremena strategija za unapređenje peroralne primene teško rastvorljivih aktivnih supstanci. Cilj rada bio je formulacija i karakterizacija samo-mikroemulgujućih nosača na bazi smeše biokompatibilnih nejonskih surfaktanata (PEG-8 kaprilno/kaprinski gliceridi (Labrasol®) i PEG-40 hidrogenizovano ricinusovo ulje (Cremophor® RH40)) za peroralnu primenu ibuprofena i in vitro karakterizacija njihove fizičke stabilnosti i veličine kapi nakon dispergovanja u vodenim medijumima različite pH vrednosti i in vitro profila oslobađanja lekovite supstance iz nosača. Rezultati karakterizacije ukazali su na značaj vrste i koncentracije ulja i masenog odnosa upotrebljenih surfaktanata za sposobnost samo-mikroemulgovanja, kapacitet za solubilizaciju ibuprofena i njegovu brzinu oslobađanja iz nosača.
PB  - Savez hemijskih inženjera, Beograd
T2  - Hemijska industrija
T1  - Formulation and characterisation of self-microemulsifying drug delivery systems based on biocompatible nonionic surfactants
T1  - Formulacija i karakterizacija samo-mikroemulgujućih nosača lekovitih supstanci na bazi biokompatibilnih nejonskih surfaktanata
VL  - 68
IS  - 5
SP  - 565
EP  - 573
DO  - 10.2298/HEMIND130825083D
ER  - 

@article{
author = "Đekić, Ljiljana and Primorac, Marija",
year = "2014",
abstract = "Development of self-dispersing drug delivery systems (SMEDDS) is a modern strategy for oral delivery improvement of poorly soluble drugs. Self-microemulsifying drug delivery systems (SMEDDS) are isotropic mixtures of oils and hydrophilic surfactants, which form oil-in-water (o/w) microemulsions by dilution in aqueous media (e.g., gastrointestinal fluids). Formulation of SMEDDS carriers requires consideration of a large number of formulation parameters and their influences on process of self-microemulsifying and releasing of drug. The aim of this work was formulation and characterization of SMEDDS for oral administration of ibuprofen. In the experimental work, two series of potential SMEDDS were prepared (M1-M10), using surfactant (Labrasol®, Gattefosse), cosurfactant (PEG­40 hydrogenated castor (Cremophor® RH40), and oil (medium chain triglycerides (Crodamol® GTCC) and olive oil (Cropur® Olive)), at surfactant-to-cosurfactant mass ratios (Km) 9:1, 7:3, 5:5, 3:7, and 1:9, and 10 or 20% of the oil phase. Ibuprofen was dissolved in formulations in concentration of 10%. Characterization of the investigated formulations included evaluation of physical stability, self-microemulsification ability in 0.1 M HCl (pH 1.2) and phosphate buffer pH 7.2 (USP) and in vitro drug release. Formation of o/w microemulsions with the average droplet size (Z-ave) up to 100 nm, was observed in dispersions of formulations prepared with 10 mass% of medium chain triglycerides, within the entire investigated range of the Km values (M1-M5). These formulations were selected as SMEDDS. Results of characterization pointed out the importance of the type and concentration of the oil as well as the Km value for the self-microemulsing ability, as well as drug release kinetics from the investigated SMEDDS. Ibuprofen release was in accordance with the request of USP 30-NF 25 (at least 80% after 60 min) from the formulations M1 (Km 9:1) and M5 (Km 1:9). Furthermore, the ibuprofen release was completed after 10 min from formulation M1, while the release from the carrier M5 (~30%) as well as from the commercial tablets Brufen® (~55%) and soft capsules Rapidol® (~65%), examined under the same conditions, was significantly slower. The present study revealed that the formulation M1 represents a potential SMEDDS which efficiently dissolves ibuprofen in acidic media, with potential to minimize the side effects, while on introduction into alkaline intestinal environment, the drug may rapidly release from the carrier and undergo absorption., Razvoj samo-mikroemulgujućih nosača je značajna savremena strategija za unapređenje peroralne primene teško rastvorljivih aktivnih supstanci. Cilj rada bio je formulacija i karakterizacija samo-mikroemulgujućih nosača na bazi smeše biokompatibilnih nejonskih surfaktanata (PEG-8 kaprilno/kaprinski gliceridi (Labrasol®) i PEG-40 hidrogenizovano ricinusovo ulje (Cremophor® RH40)) za peroralnu primenu ibuprofena i in vitro karakterizacija njihove fizičke stabilnosti i veličine kapi nakon dispergovanja u vodenim medijumima različite pH vrednosti i in vitro profila oslobađanja lekovite supstance iz nosača. Rezultati karakterizacije ukazali su na značaj vrste i koncentracije ulja i masenog odnosa upotrebljenih surfaktanata za sposobnost samo-mikroemulgovanja, kapacitet za solubilizaciju ibuprofena i njegovu brzinu oslobađanja iz nosača.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Hemijska industrija",
title = "Formulation and characterisation of self-microemulsifying drug delivery systems based on biocompatible nonionic surfactants, Formulacija i karakterizacija samo-mikroemulgujućih nosača lekovitih supstanci na bazi biokompatibilnih nejonskih surfaktanata",
volume = "68",
number = "5",
pages = "565-573",
doi = "10.2298/HEMIND130825083D"
}

Đekić, L.,& Primorac, M.. (2014). Formulation and characterisation of self-microemulsifying drug delivery systems based on biocompatible nonionic surfactants. in Hemijska industrija
Savez hemijskih inženjera, Beograd., 68(5), 565-573.
https://doi.org/10.2298/HEMIND130825083D

Đekić L, Primorac M. Formulation and characterisation of self-microemulsifying drug delivery systems based on biocompatible nonionic surfactants. in Hemijska industrija. 2014;68(5):565-573.
doi:10.2298/HEMIND130825083D .

Đekić, Ljiljana, Primorac, Marija, "Formulation and characterisation of self-microemulsifying drug delivery systems based on biocompatible nonionic surfactants" in Hemijska industrija, 68, no. 5 (2014):565-573,
https://doi.org/10.2298/HEMIND130825083D . .

TY  - JOUR
AU  - Lazarević, J. J.
AU  - Uskoković-Marković, Snežana
AU  - Jelikić-Stankov, Milena
AU  - Radonjić, M.
AU  - Tanasković, D.
AU  - Lazarević, N.
AU  - Popović, Z. V.
PY  - 2014
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2233
AB  - We report the low-temperature Raman scattering study of racemic ibuprofen. Detailed analysis of the racemic ibuprofen crystal symmetry, related to the vibrational properties of the system, has been presented. The first principle calculations of a single ibuprofen molecule dynamical properties are compered with experimental data. Nineteen, out of 26 modes expected for the spectral region below 200 cm(-1), have been observed.
PB  - Pergamon-Elsevier Science Ltd, Oxford
T2  - Spectroscopy Letters
T1  - Intermolecular and low-frequency intramolecular Raman scattering study of racemic ibuprofen
VL  - 126
SP  - 301
EP  - 305
DO  - 10.1016/j.saa.2014.01.135
ER  - 

@article{
author = "Lazarević, J. J. and Uskoković-Marković, Snežana and Jelikić-Stankov, Milena and Radonjić, M. and Tanasković, D. and Lazarević, N. and Popović, Z. V.",
year = "2014",
abstract = "We report the low-temperature Raman scattering study of racemic ibuprofen. Detailed analysis of the racemic ibuprofen crystal symmetry, related to the vibrational properties of the system, has been presented. The first principle calculations of a single ibuprofen molecule dynamical properties are compered with experimental data. Nineteen, out of 26 modes expected for the spectral region below 200 cm(-1), have been observed.",
publisher = "Pergamon-Elsevier Science Ltd, Oxford",
journal = "Spectroscopy Letters",
title = "Intermolecular and low-frequency intramolecular Raman scattering study of racemic ibuprofen",
volume = "126",
pages = "301-305",
doi = "10.1016/j.saa.2014.01.135"
}

Lazarević, J. J., Uskoković-Marković, S., Jelikić-Stankov, M., Radonjić, M., Tanasković, D., Lazarević, N.,& Popović, Z. V.. (2014). Intermolecular and low-frequency intramolecular Raman scattering study of racemic ibuprofen. in Spectroscopy Letters
Pergamon-Elsevier Science Ltd, Oxford., 126, 301-305.
https://doi.org/10.1016/j.saa.2014.01.135

Lazarević JJ, Uskoković-Marković S, Jelikić-Stankov M, Radonjić M, Tanasković D, Lazarević N, Popović ZV. Intermolecular and low-frequency intramolecular Raman scattering study of racemic ibuprofen. in Spectroscopy Letters. 2014;126:301-305.
doi:10.1016/j.saa.2014.01.135 .

Lazarević, J. J., Uskoković-Marković, Snežana, Jelikić-Stankov, Milena, Radonjić, M., Tanasković, D., Lazarević, N., Popović, Z. V., "Intermolecular and low-frequency intramolecular Raman scattering study of racemic ibuprofen" in Spectroscopy Letters, 126 (2014):301-305,
https://doi.org/10.1016/j.saa.2014.01.135 . .

TY  - JOUR
AU  - Đekić, Ljiljana
AU  - Cirković, Violeta
AU  - Heleta, Mirjana
AU  - Krajišnik, Danina
AU  - Primorac, Marija
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1878
AB  - The present study describes a screening approach in design of microemulsion preconcentrates (self-microemulsifying systems) comprising: PEG-8 caprylic/capric glycerides (Labrasol (R)) (surfactant), PEG-40 hydrogenated castor oil (Cremophor (R) RH40) (cosurfactant) (at surfactant-to-cosurfactant mass ratios 9:1, 7:3, 5:5, 3:7 and 1:9), and 10% w/w or 20% w/w of medium-chain triglycerides or olive oil (oil). The self-microemulsifying ability of the prepared surfactant/cosurfactant/oil mixtures in water and 0.1 M HCl (pH 1.2), was evaluated by droplet size and zeta potential analysis and cross-polarized light microscopy. The formation of microemulsions was observed only in the presence of medium-chain triglycerides at surfactant-to-cosurfactant ratios 7:3 and 5:5 (in the mixtures containing 10% w/w of the oil phase) and 3:7 and 1:9 (when 20% w/w of the same oil was used). The obtained results provide new implications for development of microemulsion preconcentrates suitable as delivery systems for food and pharmaceutical applications.
PB  - Carl Hanser Verlag, Munich
T2  - Tenside Surfactants Detergents
T1  - Water-Dilutable Biocompatible Microemulsion Systems: Design and Characterisation
VL  - 50
IS  - 6
SP  - 409
EP  - 413
DO  - 10.3139/113.110272
ER  - 

@article{
author = "Đekić, Ljiljana and Cirković, Violeta and Heleta, Mirjana and Krajišnik, Danina and Primorac, Marija",
year = "2013",
abstract = "The present study describes a screening approach in design of microemulsion preconcentrates (self-microemulsifying systems) comprising: PEG-8 caprylic/capric glycerides (Labrasol (R)) (surfactant), PEG-40 hydrogenated castor oil (Cremophor (R) RH40) (cosurfactant) (at surfactant-to-cosurfactant mass ratios 9:1, 7:3, 5:5, 3:7 and 1:9), and 10% w/w or 20% w/w of medium-chain triglycerides or olive oil (oil). The self-microemulsifying ability of the prepared surfactant/cosurfactant/oil mixtures in water and 0.1 M HCl (pH 1.2), was evaluated by droplet size and zeta potential analysis and cross-polarized light microscopy. The formation of microemulsions was observed only in the presence of medium-chain triglycerides at surfactant-to-cosurfactant ratios 7:3 and 5:5 (in the mixtures containing 10% w/w of the oil phase) and 3:7 and 1:9 (when 20% w/w of the same oil was used). The obtained results provide new implications for development of microemulsion preconcentrates suitable as delivery systems for food and pharmaceutical applications.",
publisher = "Carl Hanser Verlag, Munich",
journal = "Tenside Surfactants Detergents",
title = "Water-Dilutable Biocompatible Microemulsion Systems: Design and Characterisation",
volume = "50",
number = "6",
pages = "409-413",
doi = "10.3139/113.110272"
}

Đekić, L., Cirković, V., Heleta, M., Krajišnik, D.,& Primorac, M.. (2013). Water-Dilutable Biocompatible Microemulsion Systems: Design and Characterisation. in Tenside Surfactants Detergents
Carl Hanser Verlag, Munich., 50(6), 409-413.
https://doi.org/10.3139/113.110272

Đekić L, Cirković V, Heleta M, Krajišnik D, Primorac M. Water-Dilutable Biocompatible Microemulsion Systems: Design and Characterisation. in Tenside Surfactants Detergents. 2013;50(6):409-413.
doi:10.3139/113.110272 .

Đekić, Ljiljana, Cirković, Violeta, Heleta, Mirjana, Krajišnik, Danina, Primorac, Marija, "Water-Dilutable Biocompatible Microemulsion Systems: Design and Characterisation" in Tenside Surfactants Detergents, 50, no. 6 (2013):409-413,
https://doi.org/10.3139/113.110272 . .

TY  - JOUR
AU  - Đekić, Ljiljana
AU  - Primorac, Marija
AU  - Filipić, Slavica
AU  - Agbaba, Danica
PY  - 2012
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1736
AB  - The current study investigates the performances of the multicomponent mixtures of nonionic surfactants regarding the microemulsion stabilisation, drug solubilization and in vitro drug release kinetic. The primary surfactant was PEG-8 caprylic/capric glycerides (Labrasol (R)). The cosurfactants were commercially available mixtures of octoxynol-12 and polysorbate 20 without or with the addition of PEG-40 hydrogenated castor oil (Solubilisant gamma (R) 2421 and Solubilisant gamma (R) 2429, respectively). The oil phase of microemulsions was isopropyl myristate. Phase behaviour study of the pseudo-ternary systems Labrasol (R)/cosurfactant/oil/water at surfactant-to-cosurfactant weight ratios (K-m) 40:60, 50:50 and 60:40, revealed a strong synergism in the investigated tensides mixtures for stabilisation of microemulsions containing up to 80% (w/w) of water phase at surfactant +cosurfactant-to-oil weight ratio (SCoS/O) 90:10. Solubilization of a model drug ibuprofen in concentration common for topical application (5%, w/w) was achieved at the water contents below 50% (w/w). Drug free and ibuprofen-loaded microemulsions M1-M6, containing 45% (w/w) of water phase, were prepared and characterized by polarized light microscopy, conductivity, pH, rheological and droplet size measurements. In vitro ibuprofen release kinetics from the microemulsions was investigated using paddle-over-enhancer cell method and compared with the commercial 5% (w/w) ibuprofen hydrogel product (Deep Relief (R), Mentholatum Company Ltd., USA). The investigated microemulsions were isotropic, low viscous Bingham-type liquids with the pH value (4.70-6.61) suitable for topical application. The different efficiency of the tensides mixtures for microemulsion stabilisation was observed, depending on the cosurfactant type and K-m value. Solubilisant gamma (R) 2429 as well as higher K-m (i.e., lower relative content of the cosurfactant) provided higher surfactant/cosurfactant synergism. The drug molecules were predominantly solubilized within the interface film. The amount of drug released from the formulations M3 (10.75%, w/w) and M6 (13.45%, w/w) (K-m 60: 40) was limited in comparison with the reference (22.22%, w/w) and follows the Higuchi model. Microemulsions M2 and M5 (K-m 50: 50) gave zero order drug release pattern and similar to 15% (w/w) ibuprofen released. The release profiles from microemulsions M1 and M4 (K-m 40: 60) did not fit well with the models used for analysis, although the amounts of ibuprofen released (24.47%, w/w) and 17.99% (w/w), respectively) were comparable to that of the reference hydrogel. The drug release mechanism was related with the surfactant/cosurfactant synergism, thus the lower efficiency of the tensides corresponded to the faster drug release.
PB  - Elsevier Science BV, Amsterdam
T2  - International Journal of Pharmaceutics
T1  - Investigation of surfactant/cosurfactant synergism impact on ibuprofen solubilization capacity and drug release characteristics of nonionic microemulsions
VL  - 433
IS  - 1-2
SP  - 25
EP  - 33
DO  - 10.1016/j.ijpharm.2012.04.070
ER  - 

@article{
author = "Đekić, Ljiljana and Primorac, Marija and Filipić, Slavica and Agbaba, Danica",
year = "2012",
abstract = "The current study investigates the performances of the multicomponent mixtures of nonionic surfactants regarding the microemulsion stabilisation, drug solubilization and in vitro drug release kinetic. The primary surfactant was PEG-8 caprylic/capric glycerides (Labrasol (R)). The cosurfactants were commercially available mixtures of octoxynol-12 and polysorbate 20 without or with the addition of PEG-40 hydrogenated castor oil (Solubilisant gamma (R) 2421 and Solubilisant gamma (R) 2429, respectively). The oil phase of microemulsions was isopropyl myristate. Phase behaviour study of the pseudo-ternary systems Labrasol (R)/cosurfactant/oil/water at surfactant-to-cosurfactant weight ratios (K-m) 40:60, 50:50 and 60:40, revealed a strong synergism in the investigated tensides mixtures for stabilisation of microemulsions containing up to 80% (w/w) of water phase at surfactant +cosurfactant-to-oil weight ratio (SCoS/O) 90:10. Solubilization of a model drug ibuprofen in concentration common for topical application (5%, w/w) was achieved at the water contents below 50% (w/w). Drug free and ibuprofen-loaded microemulsions M1-M6, containing 45% (w/w) of water phase, were prepared and characterized by polarized light microscopy, conductivity, pH, rheological and droplet size measurements. In vitro ibuprofen release kinetics from the microemulsions was investigated using paddle-over-enhancer cell method and compared with the commercial 5% (w/w) ibuprofen hydrogel product (Deep Relief (R), Mentholatum Company Ltd., USA). The investigated microemulsions were isotropic, low viscous Bingham-type liquids with the pH value (4.70-6.61) suitable for topical application. The different efficiency of the tensides mixtures for microemulsion stabilisation was observed, depending on the cosurfactant type and K-m value. Solubilisant gamma (R) 2429 as well as higher K-m (i.e., lower relative content of the cosurfactant) provided higher surfactant/cosurfactant synergism. The drug molecules were predominantly solubilized within the interface film. The amount of drug released from the formulations M3 (10.75%, w/w) and M6 (13.45%, w/w) (K-m 60: 40) was limited in comparison with the reference (22.22%, w/w) and follows the Higuchi model. Microemulsions M2 and M5 (K-m 50: 50) gave zero order drug release pattern and similar to 15% (w/w) ibuprofen released. The release profiles from microemulsions M1 and M4 (K-m 40: 60) did not fit well with the models used for analysis, although the amounts of ibuprofen released (24.47%, w/w) and 17.99% (w/w), respectively) were comparable to that of the reference hydrogel. The drug release mechanism was related with the surfactant/cosurfactant synergism, thus the lower efficiency of the tensides corresponded to the faster drug release.",
publisher = "Elsevier Science BV, Amsterdam",
journal = "International Journal of Pharmaceutics",
title = "Investigation of surfactant/cosurfactant synergism impact on ibuprofen solubilization capacity and drug release characteristics of nonionic microemulsions",
volume = "433",
number = "1-2",
pages = "25-33",
doi = "10.1016/j.ijpharm.2012.04.070"
}

Đekić, L., Primorac, M., Filipić, S.,& Agbaba, D.. (2012). Investigation of surfactant/cosurfactant synergism impact on ibuprofen solubilization capacity and drug release characteristics of nonionic microemulsions. in International Journal of Pharmaceutics
Elsevier Science BV, Amsterdam., 433(1-2), 25-33.
https://doi.org/10.1016/j.ijpharm.2012.04.070

Đekić L, Primorac M, Filipić S, Agbaba D. Investigation of surfactant/cosurfactant synergism impact on ibuprofen solubilization capacity and drug release characteristics of nonionic microemulsions. in International Journal of Pharmaceutics. 2012;433(1-2):25-33.
doi:10.1016/j.ijpharm.2012.04.070 .

Đekić, Ljiljana, Primorac, Marija, Filipić, Slavica, Agbaba, Danica, "Investigation of surfactant/cosurfactant synergism impact on ibuprofen solubilization capacity and drug release characteristics of nonionic microemulsions" in International Journal of Pharmaceutics, 433, no. 1-2 (2012):25-33,
https://doi.org/10.1016/j.ijpharm.2012.04.070 . .