TY  - JOUR
AU  - Ušjak, Ljuboš
AU  - Stojković, Dejan
AU  - Carević, Tamara
AU  - Milutinović, Violeta
AU  - Soković, Marina
AU  - Niketić, Marjan
AU  - Petrović, Silvana
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5417
AB  - Plants of the genus Prangos are intensively investigated as potential new sources of bioactive isolated products. In this work, the chemical composition of volatile constituents (essential oils and headspace volatiles) and dichloromethane extracts, as well as antimicrobial and antibiofilm activities of essential oils and MFDEs (methanol fractions of dichloromethane extracts) of Prangos trifida from Serbia, were investigated. Volatiles of roots, leaves, stems and fruits, and fatty acids and phytosterols in dichloromethane extracts of roots and fruits were analyzed by GC-FID-MS, whereas coumarins in MFDEs by LC–MS and some isolated coumarins by 1H-NMR. Minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations/minimum fungicidal concentrations (MBCs/MFCs) of essential oils and MFDEs were determined against 13 microorganisms. Antibiofilm activity was assessed against four microorganisms. Additionally, congo red and ergosterol binding assays were conducted to elucidate selected mechanisms of antibiofilm action in the case of Candida albicans. Total of 52 volatile constituents, 16 fatty acids, eight phytosterols and 10 coumarins were identified. Essential oils demonstrated significant activity, surpassing that of commercial food preservatives, against six tested molds from the Aspergillus, Penicillium and Trichoderma genera, as well as against bacteria Staphylococcus aureus and Bacillus cereus. Most of the oils strongly inhibited the formation of biofilms by S. aureus, Listeria monocytogenes and Escherichia coli. MFDEs exhibited noteworthy effects against B. cereus and the tested Aspergillus species, particularly A. niger, and significantly inhibited C. albicans biofilm formation. This inhibition was linked to a marked reduction in exopolysaccharide production, while antifungal mechanisms associated with ergosterol remained unaffected.
PB  - MDPI
T2  - Antibiotics
T1  - Chemical Analysis and Investigation of Antimicrobial and Antibiofilm Activities of Prangos trifida (Apiaceae)
VL  - 13
IS  - 1
SP  - 41
DO  - 10.3390/antibiotics13010041
ER  - 

@article{
author = "Ušjak, Ljuboš and Stojković, Dejan and Carević, Tamara and Milutinović, Violeta and Soković, Marina and Niketić, Marjan and Petrović, Silvana",
year = "2024",
abstract = "Plants of the genus Prangos are intensively investigated as potential new sources of bioactive isolated products. In this work, the chemical composition of volatile constituents (essential oils and headspace volatiles) and dichloromethane extracts, as well as antimicrobial and antibiofilm activities of essential oils and MFDEs (methanol fractions of dichloromethane extracts) of Prangos trifida from Serbia, were investigated. Volatiles of roots, leaves, stems and fruits, and fatty acids and phytosterols in dichloromethane extracts of roots and fruits were analyzed by GC-FID-MS, whereas coumarins in MFDEs by LC–MS and some isolated coumarins by 1H-NMR. Minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations/minimum fungicidal concentrations (MBCs/MFCs) of essential oils and MFDEs were determined against 13 microorganisms. Antibiofilm activity was assessed against four microorganisms. Additionally, congo red and ergosterol binding assays were conducted to elucidate selected mechanisms of antibiofilm action in the case of Candida albicans. Total of 52 volatile constituents, 16 fatty acids, eight phytosterols and 10 coumarins were identified. Essential oils demonstrated significant activity, surpassing that of commercial food preservatives, against six tested molds from the Aspergillus, Penicillium and Trichoderma genera, as well as against bacteria Staphylococcus aureus and Bacillus cereus. Most of the oils strongly inhibited the formation of biofilms by S. aureus, Listeria monocytogenes and Escherichia coli. MFDEs exhibited noteworthy effects against B. cereus and the tested Aspergillus species, particularly A. niger, and significantly inhibited C. albicans biofilm formation. This inhibition was linked to a marked reduction in exopolysaccharide production, while antifungal mechanisms associated with ergosterol remained unaffected.",
publisher = "MDPI",
journal = "Antibiotics",
title = "Chemical Analysis and Investigation of Antimicrobial and Antibiofilm Activities of Prangos trifida (Apiaceae)",
volume = "13",
number = "1",
pages = "41",
doi = "10.3390/antibiotics13010041"
}

Ušjak, L., Stojković, D., Carević, T., Milutinović, V., Soković, M., Niketić, M.,& Petrović, S.. (2024). Chemical Analysis and Investigation of Antimicrobial and Antibiofilm Activities of Prangos trifida (Apiaceae). in Antibiotics
MDPI., 13(1), 41.
https://doi.org/10.3390/antibiotics13010041

Ušjak L, Stojković D, Carević T, Milutinović V, Soković M, Niketić M, Petrović S. Chemical Analysis and Investigation of Antimicrobial and Antibiofilm Activities of Prangos trifida (Apiaceae). in Antibiotics. 2024;13(1):41.
doi:10.3390/antibiotics13010041 .

Ušjak, Ljuboš, Stojković, Dejan, Carević, Tamara, Milutinović, Violeta, Soković, Marina, Niketić, Marjan, Petrović, Silvana, "Chemical Analysis and Investigation of Antimicrobial and Antibiofilm Activities of Prangos trifida (Apiaceae)" in Antibiotics, 13, no. 1 (2024):41,
https://doi.org/10.3390/antibiotics13010041 . .

TY  - JOUR
AU  - Paunović, Marija
AU  - Milošević, Maja
AU  - Mitrović-Ajtić, Olivera
AU  - Veličković, Nataša
AU  - Micić, Bojana
AU  - Nedić, Olgica
AU  - Todorović, Vanja
AU  - Vučić, Vesna
AU  - Petrović, Snježana
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5598
AB  - Diets high in fat and sugar lead to metabolic syndrome (MetS) and related chronic diseases. We investigated the effects of commercially available, cold-pressed polyphenol-rich black currant (BC) and cornelian cherry (CC) juices on the prevention of MetS in Wistar rats induced by a 10-weeks high-fat high-fructose (HFF) diet. Juice consumption, either BC or CC, with a HFF diet resulted in lower serum triglycerides compared to only the HFF consumption. Both juices also mitigated the effects of HFF on the liver, pancreas, and adipose tissue, by preserving liver and pancreas histomorphology and reducing visceral fat and adipocyte size. Furthermore, supplementation with both juices reduced glucagon and up-regulated insulin expression in the pancreas of the rats on the HFF diet, whereas the BC also showed improved glucose regulation. BC juice also reduced the expression of IL-6 and hepatic inflammation compared to the group only on HFF diet. Both juices, especially BC, could be a convenient solution for the prevention of MetS in humans.
PB  - Elsevier Ltd
T2  - Heliyon
T1  - Polyphenol-rich black currant and cornelian cherry juices ameliorate metabolic syndrome induced by a high-fat high-fructose diet in Wistar rats
VL  - 10
IS  - 7
DO  - 10.1016/j.heliyon.2024.e27709
ER  - 

@article{
author = "Paunović, Marija and Milošević, Maja and Mitrović-Ajtić, Olivera and Veličković, Nataša and Micić, Bojana and Nedić, Olgica and Todorović, Vanja and Vučić, Vesna and Petrović, Snježana",
year = "2024",
abstract = "Diets high in fat and sugar lead to metabolic syndrome (MetS) and related chronic diseases. We investigated the effects of commercially available, cold-pressed polyphenol-rich black currant (BC) and cornelian cherry (CC) juices on the prevention of MetS in Wistar rats induced by a 10-weeks high-fat high-fructose (HFF) diet. Juice consumption, either BC or CC, with a HFF diet resulted in lower serum triglycerides compared to only the HFF consumption. Both juices also mitigated the effects of HFF on the liver, pancreas, and adipose tissue, by preserving liver and pancreas histomorphology and reducing visceral fat and adipocyte size. Furthermore, supplementation with both juices reduced glucagon and up-regulated insulin expression in the pancreas of the rats on the HFF diet, whereas the BC also showed improved glucose regulation. BC juice also reduced the expression of IL-6 and hepatic inflammation compared to the group only on HFF diet. Both juices, especially BC, could be a convenient solution for the prevention of MetS in humans.",
publisher = "Elsevier Ltd",
journal = "Heliyon",
title = "Polyphenol-rich black currant and cornelian cherry juices ameliorate metabolic syndrome induced by a high-fat high-fructose diet in Wistar rats",
volume = "10",
number = "7",
doi = "10.1016/j.heliyon.2024.e27709"
}

Paunović, M., Milošević, M., Mitrović-Ajtić, O., Veličković, N., Micić, B., Nedić, O., Todorović, V., Vučić, V.,& Petrović, S.. (2024). Polyphenol-rich black currant and cornelian cherry juices ameliorate metabolic syndrome induced by a high-fat high-fructose diet in Wistar rats. in Heliyon
Elsevier Ltd., 10(7).
https://doi.org/10.1016/j.heliyon.2024.e27709

Paunović M, Milošević M, Mitrović-Ajtić O, Veličković N, Micić B, Nedić O, Todorović V, Vučić V, Petrović S. Polyphenol-rich black currant and cornelian cherry juices ameliorate metabolic syndrome induced by a high-fat high-fructose diet in Wistar rats. in Heliyon. 2024;10(7).
doi:10.1016/j.heliyon.2024.e27709 .

Paunović, Marija, Milošević, Maja, Mitrović-Ajtić, Olivera, Veličković, Nataša, Micić, Bojana, Nedić, Olgica, Todorović, Vanja, Vučić, Vesna, Petrović, Snježana, "Polyphenol-rich black currant and cornelian cherry juices ameliorate metabolic syndrome induced by a high-fat high-fructose diet in Wistar rats" in Heliyon, 10, no. 7 (2024),
https://doi.org/10.1016/j.heliyon.2024.e27709 . .

TY  - JOUR
AU  - Stojić-Vukanić, Zorica
AU  - Petrušić, Marija
AU  - Pilipović, Ivan
AU  - Leposavić, Gordana
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5411
AB  - INTRODUCTION: Considering significance of mechanisms of central tolerance for development of autoimmune diseases, including experimental autoimmune encephalomyelitis (EAE), and suppressive influence of circulating proinflammatory cytokines and alterations in brain-thymus communication, characteristic for the central nervous system (CNS) autoimmune diseases, on thymopoiesis, the study interogated putative strain-based thymus-related specificities relevant for the opposite effects of ageing on susceptibility of Dark Agouti (DA) and Albino Oxford (AO) rats to EAE. METHODS: Quantitative and qualitative changes in thymopoiesis including underlying mechanisms were examined using flow cytometry and RT-qPCR quantification of mRNAs for molecules relevant for integrity of stroma and T-cell development, respectively. RESULTS: With ageing, differently from DA rats, in AO rats the surface density of CD90, a negative regulator of selection threshold, on thymocytes undergoing lineage commitment was upregulated (consistent with TGF-β expression downregulation), whereas the generation of natural CD4+CD25+Foxp3+ regulatory T cells (nTregs) was impaired reflecting differences in thymic expression of cytokines supporting their development. Additionally, specifically in old AO rats, in whom EAE development depends on IL-17-producing CD8+ T cells, their thymic differentiation was augmented, reflecting augmented thymic IL-4 expression. In turn, differently from old DA rats developing self-limiting EAE, in age-matched AO rats developing EAE of prolonged duration, EAE development led to impaired generation of nTregs and accumulation of proinflammatory, cytotoxic CD28-CD4+ T cells in the periphery. DISCUSSION: The study indicates that strain differences in age-related changes in the efficacy of central tolerance, in addition to enhanced thymic generation of CD8+ T cells prone to differentiate into IL-17-producing cells, could partly explain the opposite effect of ageing on DA and AO rat susceptibility to EAE induction. Additionally, it suggested that EAE development leading to a less efficient thymic output of CD4+ cells and nTregs in old AO rats than their DA counterparts could contribute to prolonged EAE duration in AO compared with DA rats. CONCLUSION: The study warns to caution when designing therapeutic interventions to enhance thymic activity in genetically diverse populations, e.g., humans, and interpreting their outcomes. Furthermore, it indicates that CNS autoimmune pathology may additionally worsen thymic involution and age-related immune changes.
PB  - S. Karger AG, Basel.
T2  - Neuroimmunomodulation
T1  - Ageing Affects Thymopoiesis and Experimental Autoimmune Encephalomyelitis Development in a Strain-Dependent Manner
VL  - 30
IS  - 1
SP  - 346
EP  - 373
DO  - 10.1159/000535150
ER  - 

@article{
author = "Stojić-Vukanić, Zorica and Petrušić, Marija and Pilipović, Ivan and Leposavić, Gordana",
year = "2023",
abstract = "INTRODUCTION: Considering significance of mechanisms of central tolerance for development of autoimmune diseases, including experimental autoimmune encephalomyelitis (EAE), and suppressive influence of circulating proinflammatory cytokines and alterations in brain-thymus communication, characteristic for the central nervous system (CNS) autoimmune diseases, on thymopoiesis, the study interogated putative strain-based thymus-related specificities relevant for the opposite effects of ageing on susceptibility of Dark Agouti (DA) and Albino Oxford (AO) rats to EAE. METHODS: Quantitative and qualitative changes in thymopoiesis including underlying mechanisms were examined using flow cytometry and RT-qPCR quantification of mRNAs for molecules relevant for integrity of stroma and T-cell development, respectively. RESULTS: With ageing, differently from DA rats, in AO rats the surface density of CD90, a negative regulator of selection threshold, on thymocytes undergoing lineage commitment was upregulated (consistent with TGF-β expression downregulation), whereas the generation of natural CD4+CD25+Foxp3+ regulatory T cells (nTregs) was impaired reflecting differences in thymic expression of cytokines supporting their development. Additionally, specifically in old AO rats, in whom EAE development depends on IL-17-producing CD8+ T cells, their thymic differentiation was augmented, reflecting augmented thymic IL-4 expression. In turn, differently from old DA rats developing self-limiting EAE, in age-matched AO rats developing EAE of prolonged duration, EAE development led to impaired generation of nTregs and accumulation of proinflammatory, cytotoxic CD28-CD4+ T cells in the periphery. DISCUSSION: The study indicates that strain differences in age-related changes in the efficacy of central tolerance, in addition to enhanced thymic generation of CD8+ T cells prone to differentiate into IL-17-producing cells, could partly explain the opposite effect of ageing on DA and AO rat susceptibility to EAE induction. Additionally, it suggested that EAE development leading to a less efficient thymic output of CD4+ cells and nTregs in old AO rats than their DA counterparts could contribute to prolonged EAE duration in AO compared with DA rats. CONCLUSION: The study warns to caution when designing therapeutic interventions to enhance thymic activity in genetically diverse populations, e.g., humans, and interpreting their outcomes. Furthermore, it indicates that CNS autoimmune pathology may additionally worsen thymic involution and age-related immune changes.",
publisher = "S. Karger AG, Basel.",
journal = "Neuroimmunomodulation",
title = "Ageing Affects Thymopoiesis and Experimental Autoimmune Encephalomyelitis Development in a Strain-Dependent Manner",
volume = "30",
number = "1",
pages = "346-373",
doi = "10.1159/000535150"
}

Stojić-Vukanić, Z., Petrušić, M., Pilipović, I.,& Leposavić, G.. (2023). Ageing Affects Thymopoiesis and Experimental Autoimmune Encephalomyelitis Development in a Strain-Dependent Manner. in Neuroimmunomodulation
S. Karger AG, Basel.., 30(1), 346-373.
https://doi.org/10.1159/000535150

Stojić-Vukanić Z, Petrušić M, Pilipović I, Leposavić G. Ageing Affects Thymopoiesis and Experimental Autoimmune Encephalomyelitis Development in a Strain-Dependent Manner. in Neuroimmunomodulation. 2023;30(1):346-373.
doi:10.1159/000535150 .

Stojić-Vukanić, Zorica, Petrušić, Marija, Pilipović, Ivan, Leposavić, Gordana, "Ageing Affects Thymopoiesis and Experimental Autoimmune Encephalomyelitis Development in a Strain-Dependent Manner" in Neuroimmunomodulation, 30, no. 1 (2023):346-373,
https://doi.org/10.1159/000535150 . .

TY  - JOUR
AU  - Jovanović Macura, Irena
AU  - Živanović, Ana
AU  - Perović, Milka
AU  - Ćirić, Jelena
AU  - Major, Tamara
AU  - Kanazir, Selma
AU  - Ivković, Sanja
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5121
AB  - Cerebral amyloid angiopathy (CAA) is characterized by amyloid β (Aβ) accumulation in the blood vessels and is associated with cognitive impairment in Alzheimer’s disease (AD). The increased accumulation of Aβ is also present in the retinal blood vessels and a significant correlation between retinal and brain amyloid deposition was demonstrated in living patients and animal AD models. The Aβ accumulation in the retinal blood vessels can be the result of impaired transcytosis and/or the dysfunctional ocular glymphatic system in AD and during aging. We analyzed the changes in the mRNA and protein expression of major facilitator superfamily domain-containing protein2a (Mfsd2a), the major regulator of transcytosis, and of Aquaporin4 (Aqp4), the key player implicated in the functioning of the glymphatic system, in the retinas of 4- and 12-month-old WT and 5xFAD female mice. A strong decrease in the Mfsd2a mRNA and protein expression was observed in the 4 M and 12 M 5xFAD and 12 M WT retinas. The increase in the expression of srebp1-c could be at least partially responsible for the Mfsd2a decrease in the 4 M 5xFAD retinas. The decrease in the pericyte (CD13+) coverage of retinal blood vessels in the 4 M and 12 M 5xFAD retinas and in the 12 M WT retinas suggests that pericyte loss could be associated with the Mfsd2a downregulation in these experimental groups. The observed increase in Aqp4 expression in 4 M and 12 M 5xFAD and 12 M WT retinas accompanied by the decreased perivascular Aqp4 expression is indicative of the impaired glymphatic system. The findings in this study reveal the impaired Mfsd2a and Aqp4 expression and Aqp4 perivascular mislocalization in retinal blood vessels during physiological (WT) and pathological (5xFAD) aging, indicating their importance as putative targets for the development of new treatments that can improve the regulation of transcytosis or the function of the glymphatic system.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - The Expression of Major Facilitator Superfamily Domain-Containing Protein2a (Mfsd2a) and Aquaporin 4 Is Altered in the Retinas of a 5xFAD Mouse Model of Alzheimer’s Disease
VL  - 24
IS  - 18
DO  - 10.3390/ijms241814092
ER  - 

@article{
author = "Jovanović Macura, Irena and Živanović, Ana and Perović, Milka and Ćirić, Jelena and Major, Tamara and Kanazir, Selma and Ivković, Sanja",
year = "2023",
abstract = "Cerebral amyloid angiopathy (CAA) is characterized by amyloid β (Aβ) accumulation in the blood vessels and is associated with cognitive impairment in Alzheimer’s disease (AD). The increased accumulation of Aβ is also present in the retinal blood vessels and a significant correlation between retinal and brain amyloid deposition was demonstrated in living patients and animal AD models. The Aβ accumulation in the retinal blood vessels can be the result of impaired transcytosis and/or the dysfunctional ocular glymphatic system in AD and during aging. We analyzed the changes in the mRNA and protein expression of major facilitator superfamily domain-containing protein2a (Mfsd2a), the major regulator of transcytosis, and of Aquaporin4 (Aqp4), the key player implicated in the functioning of the glymphatic system, in the retinas of 4- and 12-month-old WT and 5xFAD female mice. A strong decrease in the Mfsd2a mRNA and protein expression was observed in the 4 M and 12 M 5xFAD and 12 M WT retinas. The increase in the expression of srebp1-c could be at least partially responsible for the Mfsd2a decrease in the 4 M 5xFAD retinas. The decrease in the pericyte (CD13+) coverage of retinal blood vessels in the 4 M and 12 M 5xFAD retinas and in the 12 M WT retinas suggests that pericyte loss could be associated with the Mfsd2a downregulation in these experimental groups. The observed increase in Aqp4 expression in 4 M and 12 M 5xFAD and 12 M WT retinas accompanied by the decreased perivascular Aqp4 expression is indicative of the impaired glymphatic system. The findings in this study reveal the impaired Mfsd2a and Aqp4 expression and Aqp4 perivascular mislocalization in retinal blood vessels during physiological (WT) and pathological (5xFAD) aging, indicating their importance as putative targets for the development of new treatments that can improve the regulation of transcytosis or the function of the glymphatic system.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "The Expression of Major Facilitator Superfamily Domain-Containing Protein2a (Mfsd2a) and Aquaporin 4 Is Altered in the Retinas of a 5xFAD Mouse Model of Alzheimer’s Disease",
volume = "24",
number = "18",
doi = "10.3390/ijms241814092"
}

Jovanović Macura, I., Živanović, A., Perović, M., Ćirić, J., Major, T., Kanazir, S.,& Ivković, S.. (2023). The Expression of Major Facilitator Superfamily Domain-Containing Protein2a (Mfsd2a) and Aquaporin 4 Is Altered in the Retinas of a 5xFAD Mouse Model of Alzheimer’s Disease. in International Journal of Molecular Sciences
MDPI., 24(18).
https://doi.org/10.3390/ijms241814092

Jovanović Macura I, Živanović A, Perović M, Ćirić J, Major T, Kanazir S, Ivković S. The Expression of Major Facilitator Superfamily Domain-Containing Protein2a (Mfsd2a) and Aquaporin 4 Is Altered in the Retinas of a 5xFAD Mouse Model of Alzheimer’s Disease. in International Journal of Molecular Sciences. 2023;24(18).
doi:10.3390/ijms241814092 .

Jovanović Macura, Irena, Živanović, Ana, Perović, Milka, Ćirić, Jelena, Major, Tamara, Kanazir, Selma, Ivković, Sanja, "The Expression of Major Facilitator Superfamily Domain-Containing Protein2a (Mfsd2a) and Aquaporin 4 Is Altered in the Retinas of a 5xFAD Mouse Model of Alzheimer’s Disease" in International Journal of Molecular Sciences, 24, no. 18 (2023),
https://doi.org/10.3390/ijms241814092 . .

TY  - JOUR
AU  - Jovanović-Macura, Irena
AU  - Đuričić, Ivana
AU  - Major, Tamara
AU  - Milanović, Desanka
AU  - Šobajić, Slađana
AU  - Kanazir, Selma
AU  - Ivković, Sanja
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5543
AB  - Introduction: During fetal development, the proper development of neural and visual systems relies on the maternal supplementation of omega-3 fatty acids through placental transfer. Pregnant women are strongly advised to augment their diet with additional sources of omega-3, such as fish oil (FO). This supplementation has been linked to a reduced risk of preterm birth, pre-eclampsia, and perinatal depression. Recently, higher doses of omega-3 supplementation have been recommended for pregnant women. Considering that omega-3 fatty acids, particularly docosahexaenoic acid (DHA), play a crucial role in maintaining the delicate homeostasis required for the proper functioning of the retina and photoreceptors the effects of high-dose fish oil (FO) supplementation during pregnancy and lactation on the retina and retinal pigmented epithelium (RPE) in healthy offspring warrant better understanding. Methods: The fatty acid content and the changes in the expression of the genes regulating cholesterol homeostasis and DHA transport in the retina and RPE were evaluated following the high-dose FO supplementation. Results: Our study demonstrated that despite the high-dose FO treatment during pregnancy and lactation, the rigorous DHA homeostasis in the retina and RPE of the two-month-old offspring remained balanced. Another significant finding of this study is the increase in the expression levels of major facilitator superfamily domain-containing protein (Mfsd2a), a primary DHA transporter. Mfsd2a also serves as a major regulator of transcytosis during development, and a reduction in Mfsd2a levels poses a major risk for the development of leaky blood vessels. Conclusion: Impairment of the blood-retinal barrier (BRB) is associated with the development of numerous ocular diseases, and a better understanding of how to manipulate transcytosis in the BRB during development can enhance drug delivery through the BRB or contribute to the repair of central nervous system (CNS) barriers.
PB  - Frontiers Media SA
T2  - Frontiers in Nutrition
T1  - The supplementation of a high dose of fish oil during pregnancy and lactation led to an elevation in Mfsd2a expression without any changes in docosahexaenoic acid levels in the retina of healthy 2-month-old mouse offspring
VL  - 10
SP  - 1330414
DO  - 10.3389/fnut.2023.1330414
ER  - 

@article{
author = "Jovanović-Macura, Irena and Đuričić, Ivana and Major, Tamara and Milanović, Desanka and Šobajić, Slađana and Kanazir, Selma and Ivković, Sanja",
year = "2023",
abstract = "Introduction: During fetal development, the proper development of neural and visual systems relies on the maternal supplementation of omega-3 fatty acids through placental transfer. Pregnant women are strongly advised to augment their diet with additional sources of omega-3, such as fish oil (FO). This supplementation has been linked to a reduced risk of preterm birth, pre-eclampsia, and perinatal depression. Recently, higher doses of omega-3 supplementation have been recommended for pregnant women. Considering that omega-3 fatty acids, particularly docosahexaenoic acid (DHA), play a crucial role in maintaining the delicate homeostasis required for the proper functioning of the retina and photoreceptors the effects of high-dose fish oil (FO) supplementation during pregnancy and lactation on the retina and retinal pigmented epithelium (RPE) in healthy offspring warrant better understanding. Methods: The fatty acid content and the changes in the expression of the genes regulating cholesterol homeostasis and DHA transport in the retina and RPE were evaluated following the high-dose FO supplementation. Results: Our study demonstrated that despite the high-dose FO treatment during pregnancy and lactation, the rigorous DHA homeostasis in the retina and RPE of the two-month-old offspring remained balanced. Another significant finding of this study is the increase in the expression levels of major facilitator superfamily domain-containing protein (Mfsd2a), a primary DHA transporter. Mfsd2a also serves as a major regulator of transcytosis during development, and a reduction in Mfsd2a levels poses a major risk for the development of leaky blood vessels. Conclusion: Impairment of the blood-retinal barrier (BRB) is associated with the development of numerous ocular diseases, and a better understanding of how to manipulate transcytosis in the BRB during development can enhance drug delivery through the BRB or contribute to the repair of central nervous system (CNS) barriers.",
publisher = "Frontiers Media SA",
journal = "Frontiers in Nutrition",
title = "The supplementation of a high dose of fish oil during pregnancy and lactation led to an elevation in Mfsd2a expression without any changes in docosahexaenoic acid levels in the retina of healthy 2-month-old mouse offspring",
volume = "10",
pages = "1330414",
doi = "10.3389/fnut.2023.1330414"
}

Jovanović-Macura, I., Đuričić, I., Major, T., Milanović, D., Šobajić, S., Kanazir, S.,& Ivković, S.. (2023). The supplementation of a high dose of fish oil during pregnancy and lactation led to an elevation in Mfsd2a expression without any changes in docosahexaenoic acid levels in the retina of healthy 2-month-old mouse offspring. in Frontiers in Nutrition
Frontiers Media SA., 10, 1330414.
https://doi.org/10.3389/fnut.2023.1330414

Jovanović-Macura I, Đuričić I, Major T, Milanović D, Šobajić S, Kanazir S, Ivković S. The supplementation of a high dose of fish oil during pregnancy and lactation led to an elevation in Mfsd2a expression without any changes in docosahexaenoic acid levels in the retina of healthy 2-month-old mouse offspring. in Frontiers in Nutrition. 2023;10:1330414.
doi:10.3389/fnut.2023.1330414 .

Jovanović-Macura, Irena, Đuričić, Ivana, Major, Tamara, Milanović, Desanka, Šobajić, Slađana, Kanazir, Selma, Ivković, Sanja, "The supplementation of a high dose of fish oil during pregnancy and lactation led to an elevation in Mfsd2a expression without any changes in docosahexaenoic acid levels in the retina of healthy 2-month-old mouse offspring" in Frontiers in Nutrition, 10 (2023):1330414,
https://doi.org/10.3389/fnut.2023.1330414 . .

TY  - JOUR
AU  - Ušjak, Ljuboš
AU  - Drobac, Milica
AU  - Ivanov, Marija
AU  - Soković, Marina
AU  - Milenković, Marina
AU  - Niketić, Marjan
AU  - Petrović, Silvana
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4469
AB  - Composition and antimicrobial activity of root, leaf, stem, flower and fruit essential oils from
cultivated Pastinaca sativa subsp. sativa, and its two wild-growing relatives P. sativa subsp. urens
and P. hirsuta (Apiaceae) were investigated. Twenty-nine hydrodistilled essential oils of plants from
different localities and/or years were analysed by GC-FID and GC/MS. Dominant in root oils was
myristicin (P. sativa) or apiole (P. hirsuta), in leaf and stem oils myristicin (cultivated plants) or γ-palmitolactone (wild-growing plants) and in flower and fruit oils aliphatic esters. Multivariate
statistics (PCA, nMDS, UPGMA clustering) generally revealed separation of oils of investigated
Pastinaca taxa and demonstrated their chemosystematic significance. One oil per each organ of all three plants (fifteen in total) was tested using microdilution method for activity against Candida
tropicalis, C. parapsilosis, C. krusei, C. glabrata, C. albicans, Staphylococcus aureus, Bacillus cereus, Listeria monocytogenes, Escherichia coli, Salmonella Typhimurium and Enterobacter cloacae; MIC = 0.25–8 mg/mL, MBC(MFC) = 0.5–16 mg/mL.
PB  - Taylor and Francis Group
T2  - Journal of Essential Oil Research
T1  - Composition and antimicrobial activity of Pastinaca sativa subsp. sativa, P. sativa subsp. urens and P. hirsuta essential oils
VL  - 35
IS  - 2
SP  - 154
EP  - 167
DO  - 10.1080/10412905.2022.2147675
ER  - 

@article{
author = "Ušjak, Ljuboš and Drobac, Milica and Ivanov, Marija and Soković, Marina and Milenković, Marina and Niketić, Marjan and Petrović, Silvana",
year = "2023",
abstract = "Composition and antimicrobial activity of root, leaf, stem, flower and fruit essential oils from
cultivated Pastinaca sativa subsp. sativa, and its two wild-growing relatives P. sativa subsp. urens
and P. hirsuta (Apiaceae) were investigated. Twenty-nine hydrodistilled essential oils of plants from
different localities and/or years were analysed by GC-FID and GC/MS. Dominant in root oils was
myristicin (P. sativa) or apiole (P. hirsuta), in leaf and stem oils myristicin (cultivated plants) or γ-palmitolactone (wild-growing plants) and in flower and fruit oils aliphatic esters. Multivariate
statistics (PCA, nMDS, UPGMA clustering) generally revealed separation of oils of investigated
Pastinaca taxa and demonstrated their chemosystematic significance. One oil per each organ of all three plants (fifteen in total) was tested using microdilution method for activity against Candida
tropicalis, C. parapsilosis, C. krusei, C. glabrata, C. albicans, Staphylococcus aureus, Bacillus cereus, Listeria monocytogenes, Escherichia coli, Salmonella Typhimurium and Enterobacter cloacae; MIC = 0.25–8 mg/mL, MBC(MFC) = 0.5–16 mg/mL.",
publisher = "Taylor and Francis Group",
journal = "Journal of Essential Oil Research",
title = "Composition and antimicrobial activity of Pastinaca sativa subsp. sativa, P. sativa subsp. urens and P. hirsuta essential oils",
volume = "35",
number = "2",
pages = "154-167",
doi = "10.1080/10412905.2022.2147675"
}

Ušjak, L., Drobac, M., Ivanov, M., Soković, M., Milenković, M., Niketić, M.,& Petrović, S.. (2023). Composition and antimicrobial activity of Pastinaca sativa subsp. sativa, P. sativa subsp. urens and P. hirsuta essential oils. in Journal of Essential Oil Research
Taylor and Francis Group., 35(2), 154-167.
https://doi.org/10.1080/10412905.2022.2147675

Ušjak L, Drobac M, Ivanov M, Soković M, Milenković M, Niketić M, Petrović S. Composition and antimicrobial activity of Pastinaca sativa subsp. sativa, P. sativa subsp. urens and P. hirsuta essential oils. in Journal of Essential Oil Research. 2023;35(2):154-167.
doi:10.1080/10412905.2022.2147675 .

Ušjak, Ljuboš, Drobac, Milica, Ivanov, Marija, Soković, Marina, Milenković, Marina, Niketić, Marjan, Petrović, Silvana, "Composition and antimicrobial activity of Pastinaca sativa subsp. sativa, P. sativa subsp. urens and P. hirsuta essential oils" in Journal of Essential Oil Research, 35, no. 2 (2023):154-167,
https://doi.org/10.1080/10412905.2022.2147675 . .

TY  - JOUR
AU  - Popović Minić, Dušanka
AU  - Milinčić, Danijel
AU  - Kolašinac, Stefan
AU  - Rac, Vladislav
AU  - Petrović, Jovana
AU  - Soković, Marina
AU  - Banjac, Nebojša
AU  - Lađarević, Jelena
AU  - Vidović, Bojana
AU  - Kostić, Aleksandar
AU  - Pavlović, Vladimir
AU  - Pešić, Mirjana
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4276
AB  - This study aimed to characterize mixtures of goat milk proteins and Agaricus blazei Murrill (ABM) extracts (aqueous, AE and polysaccharides, PE). The mixtures showed stable particles with ζ-potential more negative than −41.1 mV. The addition of AMB extracts to goat milk did not result in a significant particle size change, whereas their addition to heated goat milk significantly increased mean particle diameter (from 194 nm to 225 nm). Fourier Transform Infrared Spectroscopy (FTIR) showed that ABM extracts provoked changes in the secondary structure of goat milk proteins and interactions between polysaccharides and milk proteins predominantly via hydrogen and/or glycoside bonds and hydrophobic interactions. The milk protein profiles revealed proteolytic activity in mixtures with AE resulting in the formation of five new polypeptides. The different microstructures of mixtures with AE and PE were found by Scanning Electron Microscopy (SEM). A schematic representation of possible milk proteins-ABM extracts interactions has been given.
PB  - Elsevier Ltd
T2  - Food Chemistry
T1  - Goat milk proteins enriched with Agaricus blazei Murrill ss. Heinem extracts: Electrophoretic, FTIR, DLS and microstructure characterization
VL  - 402
DO  - 10.1016/j.foodchem.2022.134299
ER  - 

@article{
author = "Popović Minić, Dušanka and Milinčić, Danijel and Kolašinac, Stefan and Rac, Vladislav and Petrović, Jovana and Soković, Marina and Banjac, Nebojša and Lađarević, Jelena and Vidović, Bojana and Kostić, Aleksandar and Pavlović, Vladimir and Pešić, Mirjana",
year = "2023",
abstract = "This study aimed to characterize mixtures of goat milk proteins and Agaricus blazei Murrill (ABM) extracts (aqueous, AE and polysaccharides, PE). The mixtures showed stable particles with ζ-potential more negative than −41.1 mV. The addition of AMB extracts to goat milk did not result in a significant particle size change, whereas their addition to heated goat milk significantly increased mean particle diameter (from 194 nm to 225 nm). Fourier Transform Infrared Spectroscopy (FTIR) showed that ABM extracts provoked changes in the secondary structure of goat milk proteins and interactions between polysaccharides and milk proteins predominantly via hydrogen and/or glycoside bonds and hydrophobic interactions. The milk protein profiles revealed proteolytic activity in mixtures with AE resulting in the formation of five new polypeptides. The different microstructures of mixtures with AE and PE were found by Scanning Electron Microscopy (SEM). A schematic representation of possible milk proteins-ABM extracts interactions has been given.",
publisher = "Elsevier Ltd",
journal = "Food Chemistry",
title = "Goat milk proteins enriched with Agaricus blazei Murrill ss. Heinem extracts: Electrophoretic, FTIR, DLS and microstructure characterization",
volume = "402",
doi = "10.1016/j.foodchem.2022.134299"
}

Popović Minić, D., Milinčić, D., Kolašinac, S., Rac, V., Petrović, J., Soković, M., Banjac, N., Lađarević, J., Vidović, B., Kostić, A., Pavlović, V.,& Pešić, M.. (2023). Goat milk proteins enriched with Agaricus blazei Murrill ss. Heinem extracts: Electrophoretic, FTIR, DLS and microstructure characterization. in Food Chemistry
Elsevier Ltd., 402.
https://doi.org/10.1016/j.foodchem.2022.134299

Popović Minić D, Milinčić D, Kolašinac S, Rac V, Petrović J, Soković M, Banjac N, Lađarević J, Vidović B, Kostić A, Pavlović V, Pešić M. Goat milk proteins enriched with Agaricus blazei Murrill ss. Heinem extracts: Electrophoretic, FTIR, DLS and microstructure characterization. in Food Chemistry. 2023;402.
doi:10.1016/j.foodchem.2022.134299 .

Popović Minić, Dušanka, Milinčić, Danijel, Kolašinac, Stefan, Rac, Vladislav, Petrović, Jovana, Soković, Marina, Banjac, Nebojša, Lađarević, Jelena, Vidović, Bojana, Kostić, Aleksandar, Pavlović, Vladimir, Pešić, Mirjana, "Goat milk proteins enriched with Agaricus blazei Murrill ss. Heinem extracts: Electrophoretic, FTIR, DLS and microstructure characterization" in Food Chemistry, 402 (2023),
https://doi.org/10.1016/j.foodchem.2022.134299 . .

TY  - JOUR
AU  - Jovanović Macura, Irena
AU  - Đuričić, Ivana
AU  - Major, Tamara
AU  - Milanović, Desanka
AU  - Brkić, Marjana
AU  - Šobajić, Slađana
AU  - Kanazir, Selma
AU  - Ivković, Sanja
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4309
AB  - The recommended fish oil (FO) supplementation doses often yield low omega-3 polyunsaturated fatty acids (PUFAs) tissue bioavailability, and higher doses (up to 10 g per day) have been increasingly recommended. However, the exact effects of such FO supplementation on the healthy retina and retinal pigmented epithelium (RPE) are unknown. Our study showed that the high dose FO treatment did not imbalance the rigorous docosahexaenoic acid (DHA, C22:6n3) homeostasis in the retina and RPE in the three-month-old female B6/SLJ mice. Instead, we have found the significant increase in the expression of Mfsd2a, the main DHA transporter. Mfsd2a is also an essential regulator of blood vessel transcytosis and the decrease in Mfsd2a expression can be a risk factor for developing leaky blood vessels. Therefore, the high-dose FO supplementation emerges as the prophylactic fortifier of the retinal blood vessels.
PB  - Elsevier Ltd
T2  - Journal of Functional Foods
T1  - The high-dose fish oil supplementation increased Mfsd2a expression without altering DHA levels in the retina of healthy mice
VL  - 99
DO  - 10.1016/j.jff.2022.105302
ER  - 

@article{
author = "Jovanović Macura, Irena and Đuričić, Ivana and Major, Tamara and Milanović, Desanka and Brkić, Marjana and Šobajić, Slađana and Kanazir, Selma and Ivković, Sanja",
year = "2022",
abstract = "The recommended fish oil (FO) supplementation doses often yield low omega-3 polyunsaturated fatty acids (PUFAs) tissue bioavailability, and higher doses (up to 10 g per day) have been increasingly recommended. However, the exact effects of such FO supplementation on the healthy retina and retinal pigmented epithelium (RPE) are unknown. Our study showed that the high dose FO treatment did not imbalance the rigorous docosahexaenoic acid (DHA, C22:6n3) homeostasis in the retina and RPE in the three-month-old female B6/SLJ mice. Instead, we have found the significant increase in the expression of Mfsd2a, the main DHA transporter. Mfsd2a is also an essential regulator of blood vessel transcytosis and the decrease in Mfsd2a expression can be a risk factor for developing leaky blood vessels. Therefore, the high-dose FO supplementation emerges as the prophylactic fortifier of the retinal blood vessels.",
publisher = "Elsevier Ltd",
journal = "Journal of Functional Foods",
title = "The high-dose fish oil supplementation increased Mfsd2a expression without altering DHA levels in the retina of healthy mice",
volume = "99",
doi = "10.1016/j.jff.2022.105302"
}

Jovanović Macura, I., Đuričić, I., Major, T., Milanović, D., Brkić, M., Šobajić, S., Kanazir, S.,& Ivković, S.. (2022). The high-dose fish oil supplementation increased Mfsd2a expression without altering DHA levels in the retina of healthy mice. in Journal of Functional Foods
Elsevier Ltd., 99.
https://doi.org/10.1016/j.jff.2022.105302

Jovanović Macura I, Đuričić I, Major T, Milanović D, Brkić M, Šobajić S, Kanazir S, Ivković S. The high-dose fish oil supplementation increased Mfsd2a expression without altering DHA levels in the retina of healthy mice. in Journal of Functional Foods. 2022;99.
doi:10.1016/j.jff.2022.105302 .

Jovanović Macura, Irena, Đuričić, Ivana, Major, Tamara, Milanović, Desanka, Brkić, Marjana, Šobajić, Slađana, Kanazir, Selma, Ivković, Sanja, "The high-dose fish oil supplementation increased Mfsd2a expression without altering DHA levels in the retina of healthy mice" in Journal of Functional Foods, 99 (2022),
https://doi.org/10.1016/j.jff.2022.105302 . .

TY  - CONF
AU  - Uzelac, Branka
AU  - Stojičić, Dragana
AU  - Budimir, Snežana
AU  - Tošić, Svetlana
AU  - Zlatković, Bojan
AU  - Blagojević, Saša
AU  - Manić, Branislav
AU  - Janjanin, Mirjana
AU  - Slavkovska, Violeta
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5529
AB  - Secondary metabolism in plant plays a major role in the survival of the plant in its ecosystem, mediating the interaction of the plant with its environment. Plant bioactive compounds are biosynthesized as a defensive strategy of plants in response to natural perturbations. A number of biological effects have been associated with the main monoterpenoids detected in investigated Micromeria spp. and Clinopodium spp. essential oils. One alternative for the production of these prospective biocontrol products is in vitro plant tissue culture. Our data suggest that the metabolic potential of in vitro shoot cultures of selected species can be manipulated by varying in vitro culture conditions.
PB  - Univerzitet u Kragujevcu , Agronomski fakultet u Čačku
C3  - XXVII Savetovanje o biotehnologiji 2022, Zbornik radova
T1  - Essential oils as potential biocontrol products against plant pathogens and weeds: in vitro culture approach
SP  - 345
EP  - 350
DO  - 10.46793/SBT27.345U
ER  - 

@conference{
author = "Uzelac, Branka and Stojičić, Dragana and Budimir, Snežana and Tošić, Svetlana and Zlatković, Bojan and Blagojević, Saša and Manić, Branislav and Janjanin, Mirjana and Slavkovska, Violeta",
year = "2022",
abstract = "Secondary metabolism in plant plays a major role in the survival of the plant in its ecosystem, mediating the interaction of the plant with its environment. Plant bioactive compounds are biosynthesized as a defensive strategy of plants in response to natural perturbations. A number of biological effects have been associated with the main monoterpenoids detected in investigated Micromeria spp. and Clinopodium spp. essential oils. One alternative for the production of these prospective biocontrol products is in vitro plant tissue culture. Our data suggest that the metabolic potential of in vitro shoot cultures of selected species can be manipulated by varying in vitro culture conditions.",
publisher = "Univerzitet u Kragujevcu , Agronomski fakultet u Čačku",
journal = "XXVII Savetovanje o biotehnologiji 2022, Zbornik radova",
title = "Essential oils as potential biocontrol products against plant pathogens and weeds: in vitro culture approach",
pages = "345-350",
doi = "10.46793/SBT27.345U"
}

Uzelac, B., Stojičić, D., Budimir, S., Tošić, S., Zlatković, B., Blagojević, S., Manić, B., Janjanin, M.,& Slavkovska, V.. (2022). Essential oils as potential biocontrol products against plant pathogens and weeds: in vitro culture approach. in XXVII Savetovanje o biotehnologiji 2022, Zbornik radova
Univerzitet u Kragujevcu , Agronomski fakultet u Čačku., 345-350.
https://doi.org/10.46793/SBT27.345U

Uzelac B, Stojičić D, Budimir S, Tošić S, Zlatković B, Blagojević S, Manić B, Janjanin M, Slavkovska V. Essential oils as potential biocontrol products against plant pathogens and weeds: in vitro culture approach. in XXVII Savetovanje o biotehnologiji 2022, Zbornik radova. 2022;:345-350.
doi:10.46793/SBT27.345U .

Uzelac, Branka, Stojičić, Dragana, Budimir, Snežana, Tošić, Svetlana, Zlatković, Bojan, Blagojević, Saša, Manić, Branislav, Janjanin, Mirjana, Slavkovska, Violeta, "Essential oils as potential biocontrol products against plant pathogens and weeds: in vitro culture approach" in XXVII Savetovanje o biotehnologiji 2022, Zbornik radova (2022):345-350,
https://doi.org/10.46793/SBT27.345U . .

TY  - JOUR
AU  - Milutinović, Violeta
AU  - Matić, Ivana Z.
AU  - Stanojković, Tatjana
AU  - Soković, Marina
AU  - Ćirić, Ana D.
AU  - Ušjak, Ljuboš
AU  - Niketić, Marjan S.
AU  - Petrović, Silvana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4172
AB  - Antimicrobial and cytotoxic activities were tested for dried MeOH extracts of Hieracium calophyllum (CAL), H. coloriscapum (COL), H. pseudoschenkii (PSE), H. valdepilosum (VAL) and H. glabratum (GLA) herbs (flowering aerial parts), their 2 sesquiterpene lactones (SLs) 8-epiixerisamine A and crepiside E, and dried CH2Cl2 extract of H. scheppigianum (SCH) herb. In microdilution test, extracts showed activity on all tested microorganisms (8 bacteria, 10 fungi). The best effect was exhibited by SCH and CAL on Salmonella Typhimurium (MIC=1.7–2.5 mg/mL MBC=3.4–5.0 mg/mL), and SCH and VAL on Candida albicans (MIC=2.5 mg/mL MFC=5.0 mg/mL). SLs showed notable effect on all tested fungi Aspergillus ochraceus, Penicillium funiculosum, C. albicans and C. krusei (MIC=0.15–0.4 mg/mL MFC=0.3–0.8 mg/mL). In MTT test, extracts inhibited growth of all tested cancer cells (HeLa, LS174 and A549), with the best effect on HeLa (IC50=148.1 μg/mL for SCH, and 152.3–303.2 μg/mL for MeOH extracts); both SLs were active against HeLa cells (IC50=46.2 μg/mL for crepiside E and 103.8 μg/mL for 8-epiixerisamine A). Extracts and SLs showed good safety profile on normal MRC-5 cells.
PB  - John Wiley and Sons Inc
T2  - Chemistry and Biodiversity
T1  - Antimicrobial and Cytotoxic Activities of Selected Hieracium L. s. str. (Asteraceae) Extracts and Isolated Sesquiterpene Lactones
VL  - 19
IS  - 7
SP  - e202200326
DO  - 10.1002/cbdv.202200326
ER  - 

@article{
author = "Milutinović, Violeta and Matić, Ivana Z. and Stanojković, Tatjana and Soković, Marina and Ćirić, Ana D. and Ušjak, Ljuboš and Niketić, Marjan S. and Petrović, Silvana",
year = "2022",
abstract = "Antimicrobial and cytotoxic activities were tested for dried MeOH extracts of Hieracium calophyllum (CAL), H. coloriscapum (COL), H. pseudoschenkii (PSE), H. valdepilosum (VAL) and H. glabratum (GLA) herbs (flowering aerial parts), their 2 sesquiterpene lactones (SLs) 8-epiixerisamine A and crepiside E, and dried CH2Cl2 extract of H. scheppigianum (SCH) herb. In microdilution test, extracts showed activity on all tested microorganisms (8 bacteria, 10 fungi). The best effect was exhibited by SCH and CAL on Salmonella Typhimurium (MIC=1.7–2.5 mg/mL MBC=3.4–5.0 mg/mL), and SCH and VAL on Candida albicans (MIC=2.5 mg/mL MFC=5.0 mg/mL). SLs showed notable effect on all tested fungi Aspergillus ochraceus, Penicillium funiculosum, C. albicans and C. krusei (MIC=0.15–0.4 mg/mL MFC=0.3–0.8 mg/mL). In MTT test, extracts inhibited growth of all tested cancer cells (HeLa, LS174 and A549), with the best effect on HeLa (IC50=148.1 μg/mL for SCH, and 152.3–303.2 μg/mL for MeOH extracts); both SLs were active against HeLa cells (IC50=46.2 μg/mL for crepiside E and 103.8 μg/mL for 8-epiixerisamine A). Extracts and SLs showed good safety profile on normal MRC-5 cells.",
publisher = "John Wiley and Sons Inc",
journal = "Chemistry and Biodiversity",
title = "Antimicrobial and Cytotoxic Activities of Selected Hieracium L. s. str. (Asteraceae) Extracts and Isolated Sesquiterpene Lactones",
volume = "19",
number = "7",
pages = "e202200326",
doi = "10.1002/cbdv.202200326"
}

Milutinović, V., Matić, I. Z., Stanojković, T., Soković, M., Ćirić, A. D., Ušjak, L., Niketić, M. S.,& Petrović, S.. (2022). Antimicrobial and Cytotoxic Activities of Selected Hieracium L. s. str. (Asteraceae) Extracts and Isolated Sesquiterpene Lactones. in Chemistry and Biodiversity
John Wiley and Sons Inc., 19(7), e202200326.
https://doi.org/10.1002/cbdv.202200326

Milutinović V, Matić IZ, Stanojković T, Soković M, Ćirić AD, Ušjak L, Niketić MS, Petrović S. Antimicrobial and Cytotoxic Activities of Selected Hieracium L. s. str. (Asteraceae) Extracts and Isolated Sesquiterpene Lactones. in Chemistry and Biodiversity. 2022;19(7):e202200326.
doi:10.1002/cbdv.202200326 .

Milutinović, Violeta, Matić, Ivana Z., Stanojković, Tatjana, Soković, Marina, Ćirić, Ana D., Ušjak, Ljuboš, Niketić, Marjan S., Petrović, Silvana, "Antimicrobial and Cytotoxic Activities of Selected Hieracium L. s. str. (Asteraceae) Extracts and Isolated Sesquiterpene Lactones" in Chemistry and Biodiversity, 19, no. 7 (2022):e202200326,
https://doi.org/10.1002/cbdv.202200326 . .

TY  - CONF
AU  - Ušjak, Ljuboš
AU  - Stojković, Dejan
AU  - Soković, Marina
AU  - Niketić, Marjan
AU  - Petrović, Silvana
PY  - 2022
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4502
AB  - In this study composition and antimicrobial activity of essential oils of roots, leaves,
stems and fruits of Prangos trifida (Mill.) Herrnst. & Heyn s.l. (Apiaceae) were investigated.
Plant material was collected in Sićevo Gorge (Kusača) in 2020. Essential oils were isolated by
hydrodistillation in Clevenger-type apparatus and analyzed by GC-FID-MS. Antimicrobial
activity was tested by microdilution method against food contaminants: Gram-positive
(Staphylococcus aureus, Bacillus cereus and Listeria monocytogenes), Gram-negative bacteria
(Escherichia coli, Enterobacter cloacae and Salmonella Typhimurium), and molds (Aspergillus
fumigatus, A. niger, A. versicolor, Penicillium funiculosum, P. verrucosum var. cyclopium and
Trichoderma harzianum); artificial food preservatives E211 and E224 were used as controls.
Dominant constituents of essential oils were monoterpenes (66.2-87.2%). Terpinolene
(36.2%) and p-cymene (11.5%) prevailed in root oil, (E)-β-ocimen (23.2%) and terpinolene
(18.1%) in leaf oil, p-cimen-8-ol (21.8%) and p-cimen (14.1%) in stem oil, and p-cimen
(25.4%) and limonene (14.4%) in fruit oil. Essential oils showed significant antifungal
activity (MIC=0.10-0.78 mg/mL; MFC=0.20-1.56 mg/mL), better compared to both tested
commercial preservatives, and interesting antibacterial activity (MIC=0.20-6.25 mg/mL;
MBC=0.39-12.5 mg/mL), which was in the case of root and stem oils better than activity of
both preservatives against S. aureus and B. cereus. It should be noted that composition of
tested oils was in accordance with composition of the oils of this plant from vicinity of
Madrid (1) and from Crimea (2), while their antimicrobial activity was tested in this work for
the first time. Obtained results justify further research of P. trifida essential oils as new
natural food preservatives.
AB  - Cilj ovog rada bio je da se ispitaju hemijski sastav i antimikrobna aktivnost etarskih
ulja korena, listova, stabljika i plodova Prangos trifida (Mill.) Herrnst. & Heyn s.l. (Apiaceae).
Biljni materijal je prikupljen na području Sićevačke klisure (Kusača) 2020. god. Etarska ulja
su dobijena destilacijom vodenom parom u aparaturi po Klevendžeru i analizirana metodom
GC-FID-MS. Antimikrobna aktivnost testirana je mikrodilucionom metodom na
kontaminantima hrane: Gram-pozitivnim (Staphylococcus aureus, Bacillus cereus i Listeria
monocytogenes), Gram-negativnim bakterijama (Escherichia coli, Enterobacter cloacae i
Salmonella Typhimurium), i plesnima (Aspergillus fumigatus, A. niger, A. versicolor,
Penicillium funiculosum, P. verrucosum var. cyclopium i Trichoderma harzianum); kao
kontrole korišćeni su sintetski konzervansi E211 i E224. Dominantni sastojci etarskih ulja
bili su monoterpeni (66,2-87,2%). Najzastupljeniji u ulju korena bili su terpinolen (36,2%) i
p-cimen (11,5%), u ulju listova (E)-β-ocimen (23,2%) i terpinolen (18,1%), u ulju stabljika p-cimen-
8-ol (21,8%) i p-cimen (14,1%), a u ulju plodova p-cimen (25,4%) i limonen (14,4%).
Etarska ulja su pokazala značajnu antifungalnu aktivnost (MIK=0,10-0,78 mg/mL;
MFK=0,20-1,56 mg/mL), bolju u poređenju sa oba testirana komercijalna konzervansa, i
interesantnu antibakterijsku aktivnost (MIK=0,20-6,25 mg/mL; MBK=0,39-12,5 mg/mL),
koja je u slučaju ulja korena i stabljika prema S. aureus i B. cereus bila bolja u odnosu na oba
konzervansa. Treba istaći da je sastav ispitivanih etarskih ulja bio u skladu sa sastavom ulja
ove biljke iz okoline Madrida (1) i sa Krima (2), dok je njihova antimikrobna aktivnost
ispitivana u ovom radu po prvi put. Dobijeni rezultati predstavljaju dobru osnovu za dalja
istraživanja etarskih ulja P. trifida kao novih prirodnih konzervanasa.
PB  - Savez farmaceutskih udruženja Srbije (SFUS)
C3  - Arhiv za farmaciju
T1  - Chemical composition and antimicrobial activity of essential oils of Prangos trifida s.l.
T1  - Hemijski sastav i antimikrobna aktivnost etarskih ulja Prangos trifida s.l.
VL  - 72
IS  - 4 suplement
SP  - 455
EP  - 456
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4502
ER  - 

@conference{
author = "Ušjak, Ljuboš and Stojković, Dejan and Soković, Marina and Niketić, Marjan and Petrović, Silvana",
year = "2022",
abstract = "In this study composition and antimicrobial activity of essential oils of roots, leaves,
stems and fruits of Prangos trifida (Mill.) Herrnst. & Heyn s.l. (Apiaceae) were investigated.
Plant material was collected in Sićevo Gorge (Kusača) in 2020. Essential oils were isolated by
hydrodistillation in Clevenger-type apparatus and analyzed by GC-FID-MS. Antimicrobial
activity was tested by microdilution method against food contaminants: Gram-positive
(Staphylococcus aureus, Bacillus cereus and Listeria monocytogenes), Gram-negative bacteria
(Escherichia coli, Enterobacter cloacae and Salmonella Typhimurium), and molds (Aspergillus
fumigatus, A. niger, A. versicolor, Penicillium funiculosum, P. verrucosum var. cyclopium and
Trichoderma harzianum); artificial food preservatives E211 and E224 were used as controls.
Dominant constituents of essential oils were monoterpenes (66.2-87.2%). Terpinolene
(36.2%) and p-cymene (11.5%) prevailed in root oil, (E)-β-ocimen (23.2%) and terpinolene
(18.1%) in leaf oil, p-cimen-8-ol (21.8%) and p-cimen (14.1%) in stem oil, and p-cimen
(25.4%) and limonene (14.4%) in fruit oil. Essential oils showed significant antifungal
activity (MIC=0.10-0.78 mg/mL; MFC=0.20-1.56 mg/mL), better compared to both tested
commercial preservatives, and interesting antibacterial activity (MIC=0.20-6.25 mg/mL;
MBC=0.39-12.5 mg/mL), which was in the case of root and stem oils better than activity of
both preservatives against S. aureus and B. cereus. It should be noted that composition of
tested oils was in accordance with composition of the oils of this plant from vicinity of
Madrid (1) and from Crimea (2), while their antimicrobial activity was tested in this work for
the first time. Obtained results justify further research of P. trifida essential oils as new
natural food preservatives., Cilj ovog rada bio je da se ispitaju hemijski sastav i antimikrobna aktivnost etarskih
ulja korena, listova, stabljika i plodova Prangos trifida (Mill.) Herrnst. & Heyn s.l. (Apiaceae).
Biljni materijal je prikupljen na području Sićevačke klisure (Kusača) 2020. god. Etarska ulja
su dobijena destilacijom vodenom parom u aparaturi po Klevendžeru i analizirana metodom
GC-FID-MS. Antimikrobna aktivnost testirana je mikrodilucionom metodom na
kontaminantima hrane: Gram-pozitivnim (Staphylococcus aureus, Bacillus cereus i Listeria
monocytogenes), Gram-negativnim bakterijama (Escherichia coli, Enterobacter cloacae i
Salmonella Typhimurium), i plesnima (Aspergillus fumigatus, A. niger, A. versicolor,
Penicillium funiculosum, P. verrucosum var. cyclopium i Trichoderma harzianum); kao
kontrole korišćeni su sintetski konzervansi E211 i E224. Dominantni sastojci etarskih ulja
bili su monoterpeni (66,2-87,2%). Najzastupljeniji u ulju korena bili su terpinolen (36,2%) i
p-cimen (11,5%), u ulju listova (E)-β-ocimen (23,2%) i terpinolen (18,1%), u ulju stabljika p-cimen-
8-ol (21,8%) i p-cimen (14,1%), a u ulju plodova p-cimen (25,4%) i limonen (14,4%).
Etarska ulja su pokazala značajnu antifungalnu aktivnost (MIK=0,10-0,78 mg/mL;
MFK=0,20-1,56 mg/mL), bolju u poređenju sa oba testirana komercijalna konzervansa, i
interesantnu antibakterijsku aktivnost (MIK=0,20-6,25 mg/mL; MBK=0,39-12,5 mg/mL),
koja je u slučaju ulja korena i stabljika prema S. aureus i B. cereus bila bolja u odnosu na oba
konzervansa. Treba istaći da je sastav ispitivanih etarskih ulja bio u skladu sa sastavom ulja
ove biljke iz okoline Madrida (1) i sa Krima (2), dok je njihova antimikrobna aktivnost
ispitivana u ovom radu po prvi put. Dobijeni rezultati predstavljaju dobru osnovu za dalja
istraživanja etarskih ulja P. trifida kao novih prirodnih konzervanasa.",
publisher = "Savez farmaceutskih udruženja Srbije (SFUS)",
journal = "Arhiv za farmaciju",
title = "Chemical composition and antimicrobial activity of essential oils of Prangos trifida s.l., Hemijski sastav i antimikrobna aktivnost etarskih ulja Prangos trifida s.l.",
volume = "72",
number = "4 suplement",
pages = "455-456",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4502"
}

Ušjak, L., Stojković, D., Soković, M., Niketić, M.,& Petrović, S.. (2022). Chemical composition and antimicrobial activity of essential oils of Prangos trifida s.l.. in Arhiv za farmaciju
Savez farmaceutskih udruženja Srbije (SFUS)., 72(4 suplement), 455-456.
https://hdl.handle.net/21.15107/rcub_farfar_4502

Ušjak L, Stojković D, Soković M, Niketić M, Petrović S. Chemical composition and antimicrobial activity of essential oils of Prangos trifida s.l.. in Arhiv za farmaciju. 2022;72(4 suplement):455-456.
https://hdl.handle.net/21.15107/rcub_farfar_4502 .

Ušjak, Ljuboš, Stojković, Dejan, Soković, Marina, Niketić, Marjan, Petrović, Silvana, "Chemical composition and antimicrobial activity of essential oils of Prangos trifida s.l." in Arhiv za farmaciju, 72, no. 4 suplement (2022):455-456,
https://hdl.handle.net/21.15107/rcub_farfar_4502 .

TY  - JOUR
AU  - Čilović-Kozarević, Ermina
AU  - Šarić-Kundalić, Broza
AU  - Ibišević, Merima
AU  - Horozić, Emir
AU  - Glamočlija, Jasmina
AU  - Soković, Marina
AU  - Arsenijević, Jelena
AU  - Maksimović, Zoran
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5107
AB  - Telekia speciosa (Schreb.) Baumg., Asteraceae, is widespread in Eastern and Central Europe and the Balkan Peninsula. Previous phytochemical investigations have revealed T. speciosa as a rich source of sesquiterpene lactone -isoalantolactone, especially in its underground parts. The aim of the present study was to analyze the essential oils from aerial and underground parts of T. speciosa and investigate their antimicrobial activity. Chemical composition of essential oils was determined by GC-FID/MS method leading to the identification of 67 compounds in total, with 15.77 % oxygenated monoterpenes, 7.77 % sesquiterpene hydrocarbons, 49.14 % oxygenated sesquiterpenes, and 12.37 % other compounds from aerial parts, and 3.80 % oxygenated monoterpenes, 3.13 % sesquiterpene hydrocarbons, 90.33 % oxygenated sesquiterpenes from underground parts essential oil. The main components from aerial parts were (E)-nerolidol (11.54 %) and caryophyllene oxide (10.54 %), while isoalantolactone was the predominant component from essential oil underground parts (83.41 %). The minimum inhibitory concentration (MIC), minimum bactericidal/fungicidal concentration of the essential oils were evaluated against six strains of bacteria and two strains of fungus using in vitro microdilution method. Both oils presented antimicrobial properties against pathogens Staphylococcus aureus, Bacilus cereus, Pseudomonas aeruginosa, Escherichia coli, and Candida albicans. Inhibition of growth of tested microorganisms by T. speciosa underground parts essential oil was achieved with MICs ranging from 1.0 to 11.0 mg mL -1 , while MICs of aerial parts essential oil varied from 4.0 to 30.0 mg mL -1 . The obtained results contribute to the knowledge of antimicrobial properties of T. speciosa, which support traditional uses underground parts of the plant.
PB  - Institut za proučavanje lekovitog bilja "Dr. Josif Pančić", Beograd
T2  - Lekovite sirovine
T1  - GC/MS analysis and antimicrobial activity of essential oils of Telekia speciosa (Schreb.) Baumg
VL  - 41
SP  - 35
EP  - 40
DO  - 10.5937/leksir2141035C
ER  - 

@article{
author = "Čilović-Kozarević, Ermina and Šarić-Kundalić, Broza and Ibišević, Merima and Horozić, Emir and Glamočlija, Jasmina and Soković, Marina and Arsenijević, Jelena and Maksimović, Zoran",
year = "2021",
abstract = "Telekia speciosa (Schreb.) Baumg., Asteraceae, is widespread in Eastern and Central Europe and the Balkan Peninsula. Previous phytochemical investigations have revealed T. speciosa as a rich source of sesquiterpene lactone -isoalantolactone, especially in its underground parts. The aim of the present study was to analyze the essential oils from aerial and underground parts of T. speciosa and investigate their antimicrobial activity. Chemical composition of essential oils was determined by GC-FID/MS method leading to the identification of 67 compounds in total, with 15.77 % oxygenated monoterpenes, 7.77 % sesquiterpene hydrocarbons, 49.14 % oxygenated sesquiterpenes, and 12.37 % other compounds from aerial parts, and 3.80 % oxygenated monoterpenes, 3.13 % sesquiterpene hydrocarbons, 90.33 % oxygenated sesquiterpenes from underground parts essential oil. The main components from aerial parts were (E)-nerolidol (11.54 %) and caryophyllene oxide (10.54 %), while isoalantolactone was the predominant component from essential oil underground parts (83.41 %). The minimum inhibitory concentration (MIC), minimum bactericidal/fungicidal concentration of the essential oils were evaluated against six strains of bacteria and two strains of fungus using in vitro microdilution method. Both oils presented antimicrobial properties against pathogens Staphylococcus aureus, Bacilus cereus, Pseudomonas aeruginosa, Escherichia coli, and Candida albicans. Inhibition of growth of tested microorganisms by T. speciosa underground parts essential oil was achieved with MICs ranging from 1.0 to 11.0 mg mL -1 , while MICs of aerial parts essential oil varied from 4.0 to 30.0 mg mL -1 . The obtained results contribute to the knowledge of antimicrobial properties of T. speciosa, which support traditional uses underground parts of the plant.",
publisher = "Institut za proučavanje lekovitog bilja "Dr. Josif Pančić", Beograd",
journal = "Lekovite sirovine",
title = "GC/MS analysis and antimicrobial activity of essential oils of Telekia speciosa (Schreb.) Baumg",
volume = "41",
pages = "35-40",
doi = "10.5937/leksir2141035C"
}

Čilović-Kozarević, E., Šarić-Kundalić, B., Ibišević, M., Horozić, E., Glamočlija, J., Soković, M., Arsenijević, J.,& Maksimović, Z.. (2021). GC/MS analysis and antimicrobial activity of essential oils of Telekia speciosa (Schreb.) Baumg. in Lekovite sirovine
Institut za proučavanje lekovitog bilja "Dr. Josif Pančić", Beograd., 41, 35-40.
https://doi.org/10.5937/leksir2141035C

Čilović-Kozarević E, Šarić-Kundalić B, Ibišević M, Horozić E, Glamočlija J, Soković M, Arsenijević J, Maksimović Z. GC/MS analysis and antimicrobial activity of essential oils of Telekia speciosa (Schreb.) Baumg. in Lekovite sirovine. 2021;41:35-40.
doi:10.5937/leksir2141035C .

Čilović-Kozarević, Ermina, Šarić-Kundalić, Broza, Ibišević, Merima, Horozić, Emir, Glamočlija, Jasmina, Soković, Marina, Arsenijević, Jelena, Maksimović, Zoran, "GC/MS analysis and antimicrobial activity of essential oils of Telekia speciosa (Schreb.) Baumg" in Lekovite sirovine, 41 (2021):35-40,
https://doi.org/10.5937/leksir2141035C . .

TY  - CONF
AU  - Ušjak, Ljuboš
AU  - Drobac, Milica
AU  - Ivanov, Marija
AU  - Soković, Marina
AU  - Milenković, Marina
AU  - Niketić, Marjan
AU  - Petrović, Silvana
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4615
AB  - INTRODUCTION:
Cultivated parsnip (Pastinaca sativa subsp. sativa L., Apiaceae) root is a well-known vegetable, common ingredient of soups, stews, salads, casseroles etc. Besides, its leaves and young shoots can be added to soups and fruits are used as a condiment. Furthermore, young shoots of wild-growing parsnips, e.g. P. sativa subsp. urens (Req. ex Godr.) Čelak., are consumed pickled
or in salads or soups and it is considered that their essential oil acts as a natural preservative.

OBJECTIVES:
To investigate and compare the antimicrobial activity of the essential oils obtained from the roots, leaves, stems, flowers and fruits of cultivated P. sativa subsp. sativa and wild-growing P. sativa subsp. urens collected in Serbia. Furthermore, the most active essential oil constituents (against the most susceptible microorganisms) were predicted in silico.

METHOD / DESIGN:
Minimum inhibitory concentrations (MICs) and minimum bactericidal/fungicidal concentrations (MBCs/MFCs) of the essential oils (isolated by hydrodistillation using Clevenger-type apparatus) were determined by microdilution method against three Gram-positive bacteria: Staphylococcus aureus ATCC 11632, Bacillus cereus clinical isolate and Listeria monocytogenes NCTC 7973, three Gram-negative bacteria: Escherichia coli ATCC 25922, Salmonella Typhimurium ATCC 13311 and Enterobacter cloacae ATCC 35030, three Candida standard strains: C. albicans ATCC 10231, C. tropicalis ATCC 750 and C. parapsilosis ATCC 22019, and three Candida isolates from oral cavity: C. albicans 475/15, C. krusei H1/16 and C. glabrata 4/6/15. Pharmacokinetic properties of the compounds present in at least one oil in the quantity ≥ 1% (determined by GC-FID and GC-MS) were initially evaluated using SwissADME web tool and molecular docking was performed using AutoDock Vina 1.1.2 (interactions were visualized using Discovery Studio Visualizer 2019).

RESULTS:
All the investigated essential oils of the two Pastinaca sativa subspecies were able to reduce the growth of different tested Candida strains (MIC range 0.25-2 mg/mL; MFC range 0.5-4 mg/mL). The most promising activity was observed for both root oils (MIC range 0.25-1 mg/mL; MFC range 0.5-2 mg/mL). Among investigated Candida strains, C. parapsilosis strain was the most sensitive to these essential oils (MIC range 0.25-1 mg/mL; MFC range 0.5-2 mg/mL). The antibacterial activity of the tested essential oils was lower compared to their anticandidal potential (MIC range 1-4 mg/mL; MBC range 2-8 mg/mL). Thirty compounds were present in at least one oil in the quantity ≥ 1%. Estimation of pharmacokinetic properties using SwissADME tool suggested that 23 of these compounds are inhibitors of some of the cytochrome P450 system isoenzymes. This fact led
to assumption that they could also act against fungal sterol 14α-demethylase (CYP51), which is a common target of antifungal drugs (e.g., ketoconazole). Thus, the compounds (3D structures downloaded from PubChem) were docked to the active site of this enzyme (downloaded from Protein Data Bank, PDB code 5TZ1). The highest affinities were predicted for sesquiterpenes
caryophyllene oxide, (E)-caryophyllene, germacrene D, α-copaene, β-bourbonene and δ-cadinene (free binding energies from -9.4 to -8.7 kcal/mol; ketoconazole -11.6 kcal/mol). These compounds were present in somewhat lower quantities in the essential oils (≤ 9.9%). For dominant compounds of the tested essential oils, e.g. myristicin, γ-palmitolactone and octyl butanoate a bit lower affinities were predicted (free binding energies from -7.3 to -5.8 kcal/mol). Tested compounds mostly docked near the heme of the enzyme and formed hydrophobic interactions with the amino acid residues of the active site. According to SwissADME tool, four of five most active compounds have low absorption from gastrointestinal tract and higher skin permeation value, while caryophyllene oxide and three dominant compounds have high absorption and lower skin permeation value (similarly to ketoconazole).

CONCLUSIONS:
Investigated parsnips represent sources of essential oils and compounds with anticandidal activity.
PB  - University Prince of Songkla, Thailand
PB  - University of Novi Sad, Faculty of Sciences, Serbia, Novi Sad
C3  - The International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference - IBSC2021 Book of Abstracts
T1  - In vitro and in silico investingation of antimicrobial activity of essential oils from two Pastinaca sativa subspecies
SP  - 188
EP  - 189
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4615
ER  - 

@conference{
author = "Ušjak, Ljuboš and Drobac, Milica and Ivanov, Marija and Soković, Marina and Milenković, Marina and Niketić, Marjan and Petrović, Silvana",
year = "2021",
abstract = "INTRODUCTION:
Cultivated parsnip (Pastinaca sativa subsp. sativa L., Apiaceae) root is a well-known vegetable, common ingredient of soups, stews, salads, casseroles etc. Besides, its leaves and young shoots can be added to soups and fruits are used as a condiment. Furthermore, young shoots of wild-growing parsnips, e.g. P. sativa subsp. urens (Req. ex Godr.) Čelak., are consumed pickled
or in salads or soups and it is considered that their essential oil acts as a natural preservative.

OBJECTIVES:
To investigate and compare the antimicrobial activity of the essential oils obtained from the roots, leaves, stems, flowers and fruits of cultivated P. sativa subsp. sativa and wild-growing P. sativa subsp. urens collected in Serbia. Furthermore, the most active essential oil constituents (against the most susceptible microorganisms) were predicted in silico.

METHOD / DESIGN:
Minimum inhibitory concentrations (MICs) and minimum bactericidal/fungicidal concentrations (MBCs/MFCs) of the essential oils (isolated by hydrodistillation using Clevenger-type apparatus) were determined by microdilution method against three Gram-positive bacteria: Staphylococcus aureus ATCC 11632, Bacillus cereus clinical isolate and Listeria monocytogenes NCTC 7973, three Gram-negative bacteria: Escherichia coli ATCC 25922, Salmonella Typhimurium ATCC 13311 and Enterobacter cloacae ATCC 35030, three Candida standard strains: C. albicans ATCC 10231, C. tropicalis ATCC 750 and C. parapsilosis ATCC 22019, and three Candida isolates from oral cavity: C. albicans 475/15, C. krusei H1/16 and C. glabrata 4/6/15. Pharmacokinetic properties of the compounds present in at least one oil in the quantity ≥ 1% (determined by GC-FID and GC-MS) were initially evaluated using SwissADME web tool and molecular docking was performed using AutoDock Vina 1.1.2 (interactions were visualized using Discovery Studio Visualizer 2019).

RESULTS:
All the investigated essential oils of the two Pastinaca sativa subspecies were able to reduce the growth of different tested Candida strains (MIC range 0.25-2 mg/mL; MFC range 0.5-4 mg/mL). The most promising activity was observed for both root oils (MIC range 0.25-1 mg/mL; MFC range 0.5-2 mg/mL). Among investigated Candida strains, C. parapsilosis strain was the most sensitive to these essential oils (MIC range 0.25-1 mg/mL; MFC range 0.5-2 mg/mL). The antibacterial activity of the tested essential oils was lower compared to their anticandidal potential (MIC range 1-4 mg/mL; MBC range 2-8 mg/mL). Thirty compounds were present in at least one oil in the quantity ≥ 1%. Estimation of pharmacokinetic properties using SwissADME tool suggested that 23 of these compounds are inhibitors of some of the cytochrome P450 system isoenzymes. This fact led
to assumption that they could also act against fungal sterol 14α-demethylase (CYP51), which is a common target of antifungal drugs (e.g., ketoconazole). Thus, the compounds (3D structures downloaded from PubChem) were docked to the active site of this enzyme (downloaded from Protein Data Bank, PDB code 5TZ1). The highest affinities were predicted for sesquiterpenes
caryophyllene oxide, (E)-caryophyllene, germacrene D, α-copaene, β-bourbonene and δ-cadinene (free binding energies from -9.4 to -8.7 kcal/mol; ketoconazole -11.6 kcal/mol). These compounds were present in somewhat lower quantities in the essential oils (≤ 9.9%). For dominant compounds of the tested essential oils, e.g. myristicin, γ-palmitolactone and octyl butanoate a bit lower affinities were predicted (free binding energies from -7.3 to -5.8 kcal/mol). Tested compounds mostly docked near the heme of the enzyme and formed hydrophobic interactions with the amino acid residues of the active site. According to SwissADME tool, four of five most active compounds have low absorption from gastrointestinal tract and higher skin permeation value, while caryophyllene oxide and three dominant compounds have high absorption and lower skin permeation value (similarly to ketoconazole).

CONCLUSIONS:
Investigated parsnips represent sources of essential oils and compounds with anticandidal activity.",
publisher = "University Prince of Songkla, Thailand, University of Novi Sad, Faculty of Sciences, Serbia, Novi Sad",
journal = "The International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference - IBSC2021 Book of Abstracts",
title = "In vitro and in silico investingation of antimicrobial activity of essential oils from two Pastinaca sativa subspecies",
pages = "188-189",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4615"
}

Ušjak, L., Drobac, M., Ivanov, M., Soković, M., Milenković, M., Niketić, M.,& Petrović, S.. (2021). In vitro and in silico investingation of antimicrobial activity of essential oils from two Pastinaca sativa subspecies. in The International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference - IBSC2021 Book of Abstracts
University Prince of Songkla, Thailand., 188-189.
https://hdl.handle.net/21.15107/rcub_farfar_4615

Ušjak L, Drobac M, Ivanov M, Soković M, Milenković M, Niketić M, Petrović S. In vitro and in silico investingation of antimicrobial activity of essential oils from two Pastinaca sativa subspecies. in The International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference - IBSC2021 Book of Abstracts. 2021;:188-189.
https://hdl.handle.net/21.15107/rcub_farfar_4615 .

Ušjak, Ljuboš, Drobac, Milica, Ivanov, Marija, Soković, Marina, Milenković, Marina, Niketić, Marjan, Petrović, Silvana, "In vitro and in silico investingation of antimicrobial activity of essential oils from two Pastinaca sativa subspecies" in The International Bioscience Conference and the 8th International PSU – UNS Bioscience Conference - IBSC2021 Book of Abstracts (2021):188-189,
https://hdl.handle.net/21.15107/rcub_farfar_4615 .

TY  - JOUR
AU  - Đuretić, Jasmina
AU  - Dimitrijević, Mirjana
AU  - Stojanović, Marija
AU  - Kotur-Stevuljević, Jelena
AU  - Hamblin, Michael R.
AU  - Micov, Ana
AU  - Stepanović-Petrović, Radica
AU  - Leposavić, Gordana
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3781
AB  - The development of collagen type II (CII)-induced arthritis (CIA), a model of rheumatoid arthritis, in rats housed in cages with bedding composed of Celliant fibres containing ceramic particles, which absorb body heat and re-emit the energy back to the body in the form of infrared radiation (+IRF rats), and those housed in cages with standard wooden shaving bedding (−IRF control rats) was examined. The appearance of the first signs of CIA was postponed, while the disease was milder (judging by the arthritic score, paw volume, and burrowing behaviour) in +IRF compared with −IRF rats. This correlated with a lower magnitude of serum anti-CII IgG antibody levels in +IRF rats, and lower production level of IL-17, the Th17 signature cytokine, in cultures of their paws. This could be partly ascribed to impaired migration of antigen-loaded CD11b + dendritic cells and their positioning within lymph nodes in +IRF rats reflecting diminished lymph node expression of CCL19 /CCL21. Additionally, as confirmed in rats with carrageenan-induced paw inflammation (CIPI), the infrared radiation from Celliant fibres, independently from immunomodulatory effects, exerted anti-inflammatory effects (judging by a shift in pro-inflammatory mediator to anti-inflammatory/immunoregulatory mediator ratio towards the latter in paw cultures) and ameliorated burrowing behaviour in CIA rats.
PB  - Nature Research
T2  - Scientific Reports
T1  - Infrared radiation from cage bedding moderates rat inflammatory and autoimmune responses in collagen-induced arthritis
VL  - 11
IS  - 1
DO  - 10.1038/s41598-021-81999-7
ER  - 

@article{
author = "Đuretić, Jasmina and Dimitrijević, Mirjana and Stojanović, Marija and Kotur-Stevuljević, Jelena and Hamblin, Michael R. and Micov, Ana and Stepanović-Petrović, Radica and Leposavić, Gordana",
year = "2021",
abstract = "The development of collagen type II (CII)-induced arthritis (CIA), a model of rheumatoid arthritis, in rats housed in cages with bedding composed of Celliant fibres containing ceramic particles, which absorb body heat and re-emit the energy back to the body in the form of infrared radiation (+IRF rats), and those housed in cages with standard wooden shaving bedding (−IRF control rats) was examined. The appearance of the first signs of CIA was postponed, while the disease was milder (judging by the arthritic score, paw volume, and burrowing behaviour) in +IRF compared with −IRF rats. This correlated with a lower magnitude of serum anti-CII IgG antibody levels in +IRF rats, and lower production level of IL-17, the Th17 signature cytokine, in cultures of their paws. This could be partly ascribed to impaired migration of antigen-loaded CD11b + dendritic cells and their positioning within lymph nodes in +IRF rats reflecting diminished lymph node expression of CCL19 /CCL21. Additionally, as confirmed in rats with carrageenan-induced paw inflammation (CIPI), the infrared radiation from Celliant fibres, independently from immunomodulatory effects, exerted anti-inflammatory effects (judging by a shift in pro-inflammatory mediator to anti-inflammatory/immunoregulatory mediator ratio towards the latter in paw cultures) and ameliorated burrowing behaviour in CIA rats.",
publisher = "Nature Research",
journal = "Scientific Reports",
title = "Infrared radiation from cage bedding moderates rat inflammatory and autoimmune responses in collagen-induced arthritis",
volume = "11",
number = "1",
doi = "10.1038/s41598-021-81999-7"
}

Đuretić, J., Dimitrijević, M., Stojanović, M., Kotur-Stevuljević, J., Hamblin, M. R., Micov, A., Stepanović-Petrović, R.,& Leposavić, G.. (2021). Infrared radiation from cage bedding moderates rat inflammatory and autoimmune responses in collagen-induced arthritis. in Scientific Reports
Nature Research., 11(1).
https://doi.org/10.1038/s41598-021-81999-7

Đuretić J, Dimitrijević M, Stojanović M, Kotur-Stevuljević J, Hamblin MR, Micov A, Stepanović-Petrović R, Leposavić G. Infrared radiation from cage bedding moderates rat inflammatory and autoimmune responses in collagen-induced arthritis. in Scientific Reports. 2021;11(1).
doi:10.1038/s41598-021-81999-7 .

Đuretić, Jasmina, Dimitrijević, Mirjana, Stojanović, Marija, Kotur-Stevuljević, Jelena, Hamblin, Michael R., Micov, Ana, Stepanović-Petrović, Radica, Leposavić, Gordana, "Infrared radiation from cage bedding moderates rat inflammatory and autoimmune responses in collagen-induced arthritis" in Scientific Reports, 11, no. 1 (2021),
https://doi.org/10.1038/s41598-021-81999-7 . .

TY  - JOUR
AU  - Stojić-Vukanić, Zorica
AU  - Pilipović, Ivan
AU  - Arsenović-Ranin, Nevena
AU  - Dimitrijević, Mirjana
AU  - Leposavić, Gordana
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3946
AB  - The incidence of multiple sclerosis (MS) and susceptibility of animals to experimental autoimmune encephalomyelitis (EAE), the most commonly used experimental model of MS, decrease with aging. Generally, autoimmune diseases develop as the ultimate outcome of an imbalance between damaging immune responses against self and regulatory immune responses (keeping the former under control). Thus, in this review the age-related changes possibly underlying this balance were discussed. Specifically, considering the central role of T cells in MS/EAE, the impact of aging on overall functional capacity (reflecting both overall count and individual functional cell properties) of self-reactive conventional T cells (Tcons) and FoxP3+ regulatory T cells (Tregs), as the most potent immunoregulatory/suppressive cells, was analyzed, as well. The analysis encompasses three distinct compartments: thymus (the primary lymphoid organ responsible for the elimination of self-reactive T cells – negative selection and the generation of Tregs, compensating for imperfections of the negative selection), peripheral blood/lymphoid tissues (“afferent” compartment), and brain/spinal cord tissues (“target” compartment). Given that the incidence of MS and susceptibility of animals to EAE are greater in women/females than in age-matched men/males, sex as independent variable was also considered. In conclusion, with aging, sex-specific alterations in the balance of self-reactive Tcons/Tregs are likely to occur not only in the thymus/”afferent” compartment, but also in the “target” compartment, reflecting multifaceted changes in both T-cell types. Their in depth understanding is important not only for envisaging effects of aging, but also for designing interventions to slow-down aging without any adverse effect on incidence of autoimmune diseases.
PB  - Elsevier B.V.
T2  - Immunology Letters
T1  - Sex-specific remodeling of T-cell compartment with aging: Implications for rat susceptibility to central nervous system autoimmune diseases
VL  - 239
SP  - 42
EP  - 59
DO  - 10.1016/j.imlet.2021.08.003
ER  - 

@article{
author = "Stojić-Vukanić, Zorica and Pilipović, Ivan and Arsenović-Ranin, Nevena and Dimitrijević, Mirjana and Leposavić, Gordana",
year = "2021",
abstract = "The incidence of multiple sclerosis (MS) and susceptibility of animals to experimental autoimmune encephalomyelitis (EAE), the most commonly used experimental model of MS, decrease with aging. Generally, autoimmune diseases develop as the ultimate outcome of an imbalance between damaging immune responses against self and regulatory immune responses (keeping the former under control). Thus, in this review the age-related changes possibly underlying this balance were discussed. Specifically, considering the central role of T cells in MS/EAE, the impact of aging on overall functional capacity (reflecting both overall count and individual functional cell properties) of self-reactive conventional T cells (Tcons) and FoxP3+ regulatory T cells (Tregs), as the most potent immunoregulatory/suppressive cells, was analyzed, as well. The analysis encompasses three distinct compartments: thymus (the primary lymphoid organ responsible for the elimination of self-reactive T cells – negative selection and the generation of Tregs, compensating for imperfections of the negative selection), peripheral blood/lymphoid tissues (“afferent” compartment), and brain/spinal cord tissues (“target” compartment). Given that the incidence of MS and susceptibility of animals to EAE are greater in women/females than in age-matched men/males, sex as independent variable was also considered. In conclusion, with aging, sex-specific alterations in the balance of self-reactive Tcons/Tregs are likely to occur not only in the thymus/”afferent” compartment, but also in the “target” compartment, reflecting multifaceted changes in both T-cell types. Their in depth understanding is important not only for envisaging effects of aging, but also for designing interventions to slow-down aging without any adverse effect on incidence of autoimmune diseases.",
publisher = "Elsevier B.V.",
journal = "Immunology Letters",
title = "Sex-specific remodeling of T-cell compartment with aging: Implications for rat susceptibility to central nervous system autoimmune diseases",
volume = "239",
pages = "42-59",
doi = "10.1016/j.imlet.2021.08.003"
}

Stojić-Vukanić, Z., Pilipović, I., Arsenović-Ranin, N., Dimitrijević, M.,& Leposavić, G.. (2021). Sex-specific remodeling of T-cell compartment with aging: Implications for rat susceptibility to central nervous system autoimmune diseases. in Immunology Letters
Elsevier B.V.., 239, 42-59.
https://doi.org/10.1016/j.imlet.2021.08.003

Stojić-Vukanić Z, Pilipović I, Arsenović-Ranin N, Dimitrijević M, Leposavić G. Sex-specific remodeling of T-cell compartment with aging: Implications for rat susceptibility to central nervous system autoimmune diseases. in Immunology Letters. 2021;239:42-59.
doi:10.1016/j.imlet.2021.08.003 .

Stojić-Vukanić, Zorica, Pilipović, Ivan, Arsenović-Ranin, Nevena, Dimitrijević, Mirjana, Leposavić, Gordana, "Sex-specific remodeling of T-cell compartment with aging: Implications for rat susceptibility to central nervous system autoimmune diseases" in Immunology Letters, 239 (2021):42-59,
https://doi.org/10.1016/j.imlet.2021.08.003 . .

TY  - JOUR
AU  - Ilijević, Konstantin
AU  - Vujanović, Dragana
AU  - Orčić, Snežana
AU  - Purać, Jelena
AU  - Kojić, Danijela
AU  - Zarić, Nenad
AU  - Gržetić, Ivan
AU  - Blagojević, Duško P.
AU  - Čelić, Tatjana V.
PY  - 2021
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3648
AB  - Honeybee colony losses have been a focus of research in the last years, due to the importance of managed honeybee colonies for economy and ecology. Different unfavorable conditions from the outside environment have a strong impact on the hive health. The majority of losses occur mainly during winter and the exact reason is not completely understood. Only a small number of studies are dealing with content of bioelements, their function and influence on honeybee physiology. The aim of the present study was to determine seasonal and spatial variations in content of bioelements and non-essential elements, in hemolymph and whole body of honeybees originating from three regions with different degrees of urbanization and industrialization. Concentrations of 16 elements were compared: macroelements (Ca, K, Mg, Na), microelements (Cu, Fe, Mn, Zn) and non-essential elements (Al, Ba, Cd, Co, Cr, Ni, Pb, Sr) in samples collected from 3 different environments: Golija (rural region), Belgrade (urban region) and Zajača (industrial region). Content of bioelements and non-essential elements in honeybees was under noticeable influence of the surrounding environment, season and degree of honeybee activity. Hemolymph was proven to be helpful in differentiating air pollution from other sources of honeybee exposure. The results of our study demonstrated that bees can be successfully used as biomonitors since we have observed statistically significant differences among observed locations, but unless compared locations are exposed to excessively different pollution pressures, it is essential that all bees should be collected at the same season.
PB  - Elsevier Inc.
T2  - Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology
T1  - Anthropogenic influence on seasonal and spatial variation in bioelements and non-essential elements in honeybees and their hemolymph
VL  - 239
DO  - 10.1016/j.cbpc.2020.108852
ER  - 

@article{
author = "Ilijević, Konstantin and Vujanović, Dragana and Orčić, Snežana and Purać, Jelena and Kojić, Danijela and Zarić, Nenad and Gržetić, Ivan and Blagojević, Duško P. and Čelić, Tatjana V.",
year = "2021",
abstract = "Honeybee colony losses have been a focus of research in the last years, due to the importance of managed honeybee colonies for economy and ecology. Different unfavorable conditions from the outside environment have a strong impact on the hive health. The majority of losses occur mainly during winter and the exact reason is not completely understood. Only a small number of studies are dealing with content of bioelements, their function and influence on honeybee physiology. The aim of the present study was to determine seasonal and spatial variations in content of bioelements and non-essential elements, in hemolymph and whole body of honeybees originating from three regions with different degrees of urbanization and industrialization. Concentrations of 16 elements were compared: macroelements (Ca, K, Mg, Na), microelements (Cu, Fe, Mn, Zn) and non-essential elements (Al, Ba, Cd, Co, Cr, Ni, Pb, Sr) in samples collected from 3 different environments: Golija (rural region), Belgrade (urban region) and Zajača (industrial region). Content of bioelements and non-essential elements in honeybees was under noticeable influence of the surrounding environment, season and degree of honeybee activity. Hemolymph was proven to be helpful in differentiating air pollution from other sources of honeybee exposure. The results of our study demonstrated that bees can be successfully used as biomonitors since we have observed statistically significant differences among observed locations, but unless compared locations are exposed to excessively different pollution pressures, it is essential that all bees should be collected at the same season.",
publisher = "Elsevier Inc.",
journal = "Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology",
title = "Anthropogenic influence on seasonal and spatial variation in bioelements and non-essential elements in honeybees and their hemolymph",
volume = "239",
doi = "10.1016/j.cbpc.2020.108852"
}

Ilijević, K., Vujanović, D., Orčić, S., Purać, J., Kojić, D., Zarić, N., Gržetić, I., Blagojević, D. P.,& Čelić, T. V.. (2021). Anthropogenic influence on seasonal and spatial variation in bioelements and non-essential elements in honeybees and their hemolymph. in Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology
Elsevier Inc.., 239.
https://doi.org/10.1016/j.cbpc.2020.108852

Ilijević K, Vujanović D, Orčić S, Purać J, Kojić D, Zarić N, Gržetić I, Blagojević DP, Čelić TV. Anthropogenic influence on seasonal and spatial variation in bioelements and non-essential elements in honeybees and their hemolymph. in Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology. 2021;239.
doi:10.1016/j.cbpc.2020.108852 .

Ilijević, Konstantin, Vujanović, Dragana, Orčić, Snežana, Purać, Jelena, Kojić, Danijela, Zarić, Nenad, Gržetić, Ivan, Blagojević, Duško P., Čelić, Tatjana V., "Anthropogenic influence on seasonal and spatial variation in bioelements and non-essential elements in honeybees and their hemolymph" in Comparative Biochemistry and Physiology Part - C: Toxicology and Pharmacology, 239 (2021),
https://doi.org/10.1016/j.cbpc.2020.108852 . .

TY  - JOUR
AU  - Dimitrijević, Mirjana
AU  - Arsenović-Ranin, Nevena
AU  - Bufan, Biljana
AU  - Nacka-Aleksić, Mirjana
AU  - Kosec, Dušan
AU  - Pilipović, Ivan
AU  - Kotur-Stevuljević, Jelena
AU  - Simić, Ljubica
AU  - Sopta, Jelena
AU  - Leposavić, Gordana
PY  - 2020
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/3714
AB  - Monocytes’ plasticity has an important role in the development of rheumatoid arthritis (RA), an autoimmune disease exhibiting greater prevalence in women. Contribution of this phenomenon to sex bias in RA severity was investigated in rat collagen-induced arthritis (CIA) model of RA. The greater severity of CIA in females (exhibiting signs of bone resorption) was accompanied by the higher blood level of advanced oxidation protein products and a more pro-oxidant profile. Consistently, in females, the greater density of giant multinuclear cells (monocytes/macrophages and osteoclasts) in inflamed joint tissue was found. This correlated with the higher frequencies of CCR2- and CX3CR1- expressing cells (precursors of inflammatory monocytes/macrophages and osteoclasts) among CD11b+ splenocytes. This in conjunction with the enhanced migratory capacity of CD11b+ monocytic cells in females compared with males could be linked with the higher frequencies of CCR2+CX3CR1-CD43lowCD11b+ and CCR2-CX3CR1+CD43hiCD11b+ cells (corresponding to “classical” and “non-classical” monocytes, respectively) and the greater density of CD68+ cells (monocytes/macrophages and osteoclast precursors/osteoclasts) in blood and inflamed paws from female rats, respectively. Consistently, the higher levels of GM-CSF, TNF-α and IL-6, IL-1β (driving Th17 cell differentiation), and IL-17 followed by the lower level of IL-10 were measured in inflamed paw cultures from female compared with male rats. To the greater IL-17 production (associated with enhanced monocyte immigration and differentiation into osteoclasts) most likely contributed augmented Th17 cell generation in the lymph nodes draining arthritic joints from female compared with male rats. Overall, the study suggests the sex-specific contribution of monocytic lineage cells to CIA, and possibly RA development.
PB  - Springer Nature
T2  - Inflammation
T1  - Sex-Based Differences in Monocytic Lineage Cells Contribute to More Severe Collagen-Induced Arthritis in Female Rats Compared with Male Rats
VL  - 43
IS  - 6
SP  - 2312
EP  - 2331
DO  - 10.1007/s10753-020-01302-0
ER  - 

@article{
author = "Dimitrijević, Mirjana and Arsenović-Ranin, Nevena and Bufan, Biljana and Nacka-Aleksić, Mirjana and Kosec, Dušan and Pilipović, Ivan and Kotur-Stevuljević, Jelena and Simić, Ljubica and Sopta, Jelena and Leposavić, Gordana",
year = "2020",
abstract = "Monocytes’ plasticity has an important role in the development of rheumatoid arthritis (RA), an autoimmune disease exhibiting greater prevalence in women. Contribution of this phenomenon to sex bias in RA severity was investigated in rat collagen-induced arthritis (CIA) model of RA. The greater severity of CIA in females (exhibiting signs of bone resorption) was accompanied by the higher blood level of advanced oxidation protein products and a more pro-oxidant profile. Consistently, in females, the greater density of giant multinuclear cells (monocytes/macrophages and osteoclasts) in inflamed joint tissue was found. This correlated with the higher frequencies of CCR2- and CX3CR1- expressing cells (precursors of inflammatory monocytes/macrophages and osteoclasts) among CD11b+ splenocytes. This in conjunction with the enhanced migratory capacity of CD11b+ monocytic cells in females compared with males could be linked with the higher frequencies of CCR2+CX3CR1-CD43lowCD11b+ and CCR2-CX3CR1+CD43hiCD11b+ cells (corresponding to “classical” and “non-classical” monocytes, respectively) and the greater density of CD68+ cells (monocytes/macrophages and osteoclast precursors/osteoclasts) in blood and inflamed paws from female rats, respectively. Consistently, the higher levels of GM-CSF, TNF-α and IL-6, IL-1β (driving Th17 cell differentiation), and IL-17 followed by the lower level of IL-10 were measured in inflamed paw cultures from female compared with male rats. To the greater IL-17 production (associated with enhanced monocyte immigration and differentiation into osteoclasts) most likely contributed augmented Th17 cell generation in the lymph nodes draining arthritic joints from female compared with male rats. Overall, the study suggests the sex-specific contribution of monocytic lineage cells to CIA, and possibly RA development.",
publisher = "Springer Nature",
journal = "Inflammation",
title = "Sex-Based Differences in Monocytic Lineage Cells Contribute to More Severe Collagen-Induced Arthritis in Female Rats Compared with Male Rats",
volume = "43",
number = "6",
pages = "2312-2331",
doi = "10.1007/s10753-020-01302-0"
}

Dimitrijević, M., Arsenović-Ranin, N., Bufan, B., Nacka-Aleksić, M., Kosec, D., Pilipović, I., Kotur-Stevuljević, J., Simić, L., Sopta, J.,& Leposavić, G.. (2020). Sex-Based Differences in Monocytic Lineage Cells Contribute to More Severe Collagen-Induced Arthritis in Female Rats Compared with Male Rats. in Inflammation
Springer Nature., 43(6), 2312-2331.
https://doi.org/10.1007/s10753-020-01302-0

Dimitrijević M, Arsenović-Ranin N, Bufan B, Nacka-Aleksić M, Kosec D, Pilipović I, Kotur-Stevuljević J, Simić L, Sopta J, Leposavić G. Sex-Based Differences in Monocytic Lineage Cells Contribute to More Severe Collagen-Induced Arthritis in Female Rats Compared with Male Rats. in Inflammation. 2020;43(6):2312-2331.
doi:10.1007/s10753-020-01302-0 .

Dimitrijević, Mirjana, Arsenović-Ranin, Nevena, Bufan, Biljana, Nacka-Aleksić, Mirjana, Kosec, Dušan, Pilipović, Ivan, Kotur-Stevuljević, Jelena, Simić, Ljubica, Sopta, Jelena, Leposavić, Gordana, "Sex-Based Differences in Monocytic Lineage Cells Contribute to More Severe Collagen-Induced Arthritis in Female Rats Compared with Male Rats" in Inflammation, 43, no. 6 (2020):2312-2331,
https://doi.org/10.1007/s10753-020-01302-0 . .