TY  - JOUR
AU  - Popović, Višnja
AU  - Heyerick, Arne
AU  - Petrović, Silvana
AU  - Van Calenbergh, Serge
AU  - Karalić, Izet
AU  - Niketić, Marjan
AU  - Deforce, Dieter
PY  - 2013
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/2000
AB  - In the search for novel cytotoxic agents, sesquiterpenoids and phenylpropanoids have provided interesting lead compounds. From the chloroform extract of the underground parts of Laserpitium latifolium, the daucane sesquiterpenoids laserpitin and acetyldesoxodehydrolaserpitin, and the phenylpropanoids laserin and latifolon were isolated as the major compounds. Acetyldesoxodehydrolaserpitin is identified for the first time in the genus Laserpitium. Using a MTT and a sulforhodamine B (SRB) assay, the cytotoxic and antiproliferative activity of the extract and compounds laserpitin, acetyldesoxodehydrolaserpitin and laserin was tested in two closely related human breast adenocarcinoma cell lines, ie. the invasive MCF 7/6 and the non-invasive MCF 7/AZ. The IC50 values of the extract were in the range of 184.72 - 397.16 mu g/mL, with the most potent effect observed in the MTT test on the MCF 7/6 line. Among the tested compounds, acetyldesoxodehydrolaserpitin exerted a most potent, concentration-dependent effect (IC50 values of 0.60 and 0.51 mu M in the MCF 7/6 cell line, and 2.29 mu M and 31.87 mu M in the MCF 7/AZ cell line in the MTT and SRB test, respectively). The effect of laserin was more pronounced in the MTT test (IC50 of 4.57 and 2.46 mu M in the MCF 7/6 and MCF 7/AZ, respectively).
PB  - ACG Publications
T2  - Records of Natural Products
T1  - Cytotoxic Activity of Laserpitium latifolium L. Extract and Its Daucane and Phenylpropanoid Constituents
VL  - 7
IS  - 3
SP  - 245
EP  - 249
UR  - https://hdl.handle.net/21.15107/rcub_farfar_2000
ER  - 

@article{
author = "Popović, Višnja and Heyerick, Arne and Petrović, Silvana and Van Calenbergh, Serge and Karalić, Izet and Niketić, Marjan and Deforce, Dieter",
year = "2013",
abstract = "In the search for novel cytotoxic agents, sesquiterpenoids and phenylpropanoids have provided interesting lead compounds. From the chloroform extract of the underground parts of Laserpitium latifolium, the daucane sesquiterpenoids laserpitin and acetyldesoxodehydrolaserpitin, and the phenylpropanoids laserin and latifolon were isolated as the major compounds. Acetyldesoxodehydrolaserpitin is identified for the first time in the genus Laserpitium. Using a MTT and a sulforhodamine B (SRB) assay, the cytotoxic and antiproliferative activity of the extract and compounds laserpitin, acetyldesoxodehydrolaserpitin and laserin was tested in two closely related human breast adenocarcinoma cell lines, ie. the invasive MCF 7/6 and the non-invasive MCF 7/AZ. The IC50 values of the extract were in the range of 184.72 - 397.16 mu g/mL, with the most potent effect observed in the MTT test on the MCF 7/6 line. Among the tested compounds, acetyldesoxodehydrolaserpitin exerted a most potent, concentration-dependent effect (IC50 values of 0.60 and 0.51 mu M in the MCF 7/6 cell line, and 2.29 mu M and 31.87 mu M in the MCF 7/AZ cell line in the MTT and SRB test, respectively). The effect of laserin was more pronounced in the MTT test (IC50 of 4.57 and 2.46 mu M in the MCF 7/6 and MCF 7/AZ, respectively).",
publisher = "ACG Publications",
journal = "Records of Natural Products",
title = "Cytotoxic Activity of Laserpitium latifolium L. Extract and Its Daucane and Phenylpropanoid Constituents",
volume = "7",
number = "3",
pages = "245-249",
url = "https://hdl.handle.net/21.15107/rcub_farfar_2000"
}

Popović, V., Heyerick, A., Petrović, S., Van Calenbergh, S., Karalić, I., Niketić, M.,& Deforce, D.. (2013). Cytotoxic Activity of Laserpitium latifolium L. Extract and Its Daucane and Phenylpropanoid Constituents. in Records of Natural Products
ACG Publications., 7(3), 245-249.
https://hdl.handle.net/21.15107/rcub_farfar_2000

Popović V, Heyerick A, Petrović S, Van Calenbergh S, Karalić I, Niketić M, Deforce D. Cytotoxic Activity of Laserpitium latifolium L. Extract and Its Daucane and Phenylpropanoid Constituents. in Records of Natural Products. 2013;7(3):245-249.
https://hdl.handle.net/21.15107/rcub_farfar_2000 .

Popović, Višnja, Heyerick, Arne, Petrović, Silvana, Van Calenbergh, Serge, Karalić, Izet, Niketić, Marjan, Deforce, Dieter, "Cytotoxic Activity of Laserpitium latifolium L. Extract and Its Daucane and Phenylpropanoid Constituents" in Records of Natural Products, 7, no. 3 (2013):245-249,
https://hdl.handle.net/21.15107/rcub_farfar_2000 .