TY  - JOUR
AU  - Čakar, Mira
AU  - Popović, Gordana
AU  - Agbaba, Danica
PY  - 2005
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/612
AB  - Simple and reliable thin-layer chromatography-densitometry methods for determination of antimycotics (bifonazole, clotrimazole, and miconazole) and preservatives (benzyl alcohol and benzoic acid) were developed. The pairs bifonazole/benzyl alcohol, clotrimazole/benzyl alcohol, and miconazole/benzoic acid were determined simultaneously. The following mobile phases were used: ethyl acetate-n-heptane-methanol-diethylamine (3 + 4.5 + 1 + 0.2, v/v/v/v) for bifonazole and benzyl alcohol; n-butyl acetate-n-heptane-methanol-dietylamine (3 + 4.5 + 1 + 0.2, v/v/v/v) for clotrimazole and benzyl alcohol; and n-butyl acetate-carbon tetrachloride-methanol-diethylamine (3 + 6 + 2.5 + 0.5, v/v/v/v) for miconazole and benzoic acid. The chromatographic zones on silica gel plates were scanned in the reflectance/absorbance mode at 230 nm (bifonazole, benzyl alcohol, miconazole, and benzoic acid) and 210 nm (clotrimazole and benzyl alcohol). The recovery for all substances ranged from 98.7 to 100.7%. The limits of detection and quantitation were 0.03 to 0.2 mu g and 0.1 to 0.5 mu g/spot, respectively. The proposed methods were applied for determination of antimycotics and preservatives in commercially available pharmaceuticals.
PB  - AOAC Int, Gaithersburg
T2  - Journal of AOAC International
T1  - High-performance thin-layer chromatography determination of some antimycotic imidazole derivatives and preservatives in medicinal creams and a gel
VL  - 88
IS  - 5
SP  - 1544
EP  - 1548
DO  - 10.1093/jaoac/88.5.1544
ER  - 

@article{
author = "Čakar, Mira and Popović, Gordana and Agbaba, Danica",
year = "2005",
abstract = "Simple and reliable thin-layer chromatography-densitometry methods for determination of antimycotics (bifonazole, clotrimazole, and miconazole) and preservatives (benzyl alcohol and benzoic acid) were developed. The pairs bifonazole/benzyl alcohol, clotrimazole/benzyl alcohol, and miconazole/benzoic acid were determined simultaneously. The following mobile phases were used: ethyl acetate-n-heptane-methanol-diethylamine (3 + 4.5 + 1 + 0.2, v/v/v/v) for bifonazole and benzyl alcohol; n-butyl acetate-n-heptane-methanol-dietylamine (3 + 4.5 + 1 + 0.2, v/v/v/v) for clotrimazole and benzyl alcohol; and n-butyl acetate-carbon tetrachloride-methanol-diethylamine (3 + 6 + 2.5 + 0.5, v/v/v/v) for miconazole and benzoic acid. The chromatographic zones on silica gel plates were scanned in the reflectance/absorbance mode at 230 nm (bifonazole, benzyl alcohol, miconazole, and benzoic acid) and 210 nm (clotrimazole and benzyl alcohol). The recovery for all substances ranged from 98.7 to 100.7%. The limits of detection and quantitation were 0.03 to 0.2 mu g and 0.1 to 0.5 mu g/spot, respectively. The proposed methods were applied for determination of antimycotics and preservatives in commercially available pharmaceuticals.",
publisher = "AOAC Int, Gaithersburg",
journal = "Journal of AOAC International",
title = "High-performance thin-layer chromatography determination of some antimycotic imidazole derivatives and preservatives in medicinal creams and a gel",
volume = "88",
number = "5",
pages = "1544-1548",
doi = "10.1093/jaoac/88.5.1544"
}

Čakar, M., Popović, G.,& Agbaba, D.. (2005). High-performance thin-layer chromatography determination of some antimycotic imidazole derivatives and preservatives in medicinal creams and a gel. in Journal of AOAC International
AOAC Int, Gaithersburg., 88(5), 1544-1548.
https://doi.org/10.1093/jaoac/88.5.1544

Čakar M, Popović G, Agbaba D. High-performance thin-layer chromatography determination of some antimycotic imidazole derivatives and preservatives in medicinal creams and a gel. in Journal of AOAC International. 2005;88(5):1544-1548.
doi:10.1093/jaoac/88.5.1544 .

Čakar, Mira, Popović, Gordana, Agbaba, Danica, "High-performance thin-layer chromatography determination of some antimycotic imidazole derivatives and preservatives in medicinal creams and a gel" in Journal of AOAC International, 88, no. 5 (2005):1544-1548,
https://doi.org/10.1093/jaoac/88.5.1544 . .