TY  - JOUR
AU  - Davidović, Lazar B.
AU  - Končar, Igor B.
AU  - Dragaš, Marko V.
AU  - Marković, Miroslav D.
AU  - Bogavac-Stanojević, Nataša
AU  - Vujčić, Aleksandra D.
AU  - Mitrović, Aleksandar C.
AU  - Ilić, Nikola S.
AU  - Trailović, R.D.
AU  - Kostić, Dušan M.
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4430
AB  - BACKGROUND: The purpose of this study was to examine demographic and baseline characteristics of patients with vascular injuries of extremities and to define the most relevant factors which influenced an early outcome, as well as limb salvage after the management of vascular trauma. METHODS: This study used the database that included 395 patients with peripheral arterial injuries, who were treated in the tertiary vascular university center in the period between 2005-2020. Exclusion criteria were isolated thoracic, abdominal and neck injuries as well as iatrogenic injuries and injuries of intravenous addicts. Univariate binary logistic regression analysis and multiple logistic regression were used to determine risk factors for lomb loss (after vascular reconstruction) or mortality. Decision to perform primary amputation (without vascular reconstruction) was based on surgeons' preference and experience. RESULTS: Out of 395 vascular injuries treated in the period 2005-2020, 210 (53.2%) presented with non-iatrogenic vascular injuries of upper and lower extremities were analyzed. According to the univariate regression analysis, hemorrhage as the main clinical manifestation on admission (P=0.035) and early reintervention (P=0.048) increased, while an early patency of repaired artery (0.010) significantly decreased a 30-day amputation rate. Multivariate logistic regression analysis of these three variables showed that only early patency of repaired artery significantly decreased the early amputation rate (P=0.009). CONCLUSIONS: Based on presented experience, the patency of vascular reconstruction plays a crucial role in limb salvage in patients with non-iatrogenic peripheral vascular injuries. All factors that might influence the patency should be in focus of improvement.
PB  - Edizioni Minerva Medica
T2  - The Journal of cardiovascular surgery
T1  - Treatment of vascular non-iatrogenic injuries of upper and lower extremities in tertiary vascular center
VL  - 64
IS  - 1
SP  - 74
EP  - 81
DO  - 10.23736/S0021-9509.22.12243-3
ER  - 

@article{
author = "Davidović, Lazar B. and Končar, Igor B. and Dragaš, Marko V. and Marković, Miroslav D. and Bogavac-Stanojević, Nataša and Vujčić, Aleksandra D. and Mitrović, Aleksandar C. and Ilić, Nikola S. and Trailović, R.D. and Kostić, Dušan M.",
abstract = "BACKGROUND: The purpose of this study was to examine demographic and baseline characteristics of patients with vascular injuries of extremities and to define the most relevant factors which influenced an early outcome, as well as limb salvage after the management of vascular trauma. METHODS: This study used the database that included 395 patients with peripheral arterial injuries, who were treated in the tertiary vascular university center in the period between 2005-2020. Exclusion criteria were isolated thoracic, abdominal and neck injuries as well as iatrogenic injuries and injuries of intravenous addicts. Univariate binary logistic regression analysis and multiple logistic regression were used to determine risk factors for lomb loss (after vascular reconstruction) or mortality. Decision to perform primary amputation (without vascular reconstruction) was based on surgeons' preference and experience. RESULTS: Out of 395 vascular injuries treated in the period 2005-2020, 210 (53.2%) presented with non-iatrogenic vascular injuries of upper and lower extremities were analyzed. According to the univariate regression analysis, hemorrhage as the main clinical manifestation on admission (P=0.035) and early reintervention (P=0.048) increased, while an early patency of repaired artery (0.010) significantly decreased a 30-day amputation rate. Multivariate logistic regression analysis of these three variables showed that only early patency of repaired artery significantly decreased the early amputation rate (P=0.009). CONCLUSIONS: Based on presented experience, the patency of vascular reconstruction plays a crucial role in limb salvage in patients with non-iatrogenic peripheral vascular injuries. All factors that might influence the patency should be in focus of improvement.",
publisher = "Edizioni Minerva Medica",
journal = "The Journal of cardiovascular surgery",
title = "Treatment of vascular non-iatrogenic injuries of upper and lower extremities in tertiary vascular center",
volume = "64",
number = "1",
pages = "74-81",
doi = "10.23736/S0021-9509.22.12243-3"
}

Davidović, L. B., Končar, I. B., Dragaš, M. V., Marković, M. D., Bogavac-Stanojević, N., Vujčić, A. D., Mitrović, A. C., Ilić, N. S., Trailović, R.D.,& Kostić, D. M..Treatment of vascular non-iatrogenic injuries of upper and lower extremities in tertiary vascular center. in The Journal of cardiovascular surgery
Edizioni Minerva Medica., 64(1), 74-81.
https://doi.org/10.23736/S0021-9509.22.12243-3

Davidović LB, Končar IB, Dragaš MV, Marković MD, Bogavac-Stanojević N, Vujčić AD, Mitrović AC, Ilić NS, Trailović R, Kostić DM. Treatment of vascular non-iatrogenic injuries of upper and lower extremities in tertiary vascular center. in The Journal of cardiovascular surgery.64(1):74-81.
doi:10.23736/S0021-9509.22.12243-3 .

Davidović, Lazar B., Končar, Igor B., Dragaš, Marko V., Marković, Miroslav D., Bogavac-Stanojević, Nataša, Vujčić, Aleksandra D., Mitrović, Aleksandar C., Ilić, Nikola S., Trailović, R.D., Kostić, Dušan M., "Treatment of vascular non-iatrogenic injuries of upper and lower extremities in tertiary vascular center" in The Journal of cardiovascular surgery, 64, no. 1:74-81,
https://doi.org/10.23736/S0021-9509.22.12243-3 . .

TY  - JOUR
AU  - Antunović, Marko
AU  - Vučinić, Slavica
AU  - Kotur-Stevuljević, Jelena
AU  - Krstić, Kristijan
AU  - Jović-Stošić, Jasmina
AU  - Kilibarda, Vesna
AU  - Perković-Vukčević, Nataša
AU  - Đorđević, Snežana
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4699
AB  - Purpose: The aim of this study was to analyze data on gabapentinoid-related attendances to the National Poison Control Center of Serbia (NPCC), particularly abuse cases; to estimate its changes and to compare it with trends in national consumption rates of these drugs. We also aimed to analyze the main characteristics of the study population and to investigate the major clinical effects in poisoned patients. Patients and Methods: This is a retrospective study of patients admitted to the NPCC for acute poisoning involving gabapentinoids from 1 May 2012 to 1 October 2022. Results: There were 357 (95.5%) pregabalin-related and 17 (4.5%) gabapentin-related poisoning cases in 302 patients. Abuse of pregabalin was detected in 27.8% (84/302), while gabapentin abuse occurred in 0.7% (2/302) of all patients. A steady increase in rates of pregabalin poisoning and abuse cases strongly correlated with the increase in overall consumption of this drug, while there were no significant changes in rates of gabapentin consumption, poisoning and abuse rate during the study period. Most patients who abused pregabalin pregabalin were males (84.5%) and the median age was 26 years (range: 15–45 years). Almost 60% of patients who abused pregabalin (48/84) belonged to the migrant population. Co-ingestions occurred in 89.4% of pregabalin-related cases (319/357), resulting in more severe poisoning. The most often co-ingested drugs were benzodiazepines and among them clonazepam was detected in the largest number of cases. Conclusion: The poisoning and abuse cases involving pregabalin are on the rise in Serbia, which coincided with an increase in its overall consumption during the study period. Isolated pregabalin ingestions resulted in mild poisoning, although severe symptoms such as coma and bradycardia were recorded. When prescribing pregabalin to patients at risk of abuse caution is needed. Strengthening the measures for dispensing of pregabalin may reduce the risks associated with its abuse.
PB  - Dove Medical Press Ltd
T2  - International Journal of General Medicine
T1  - Rise of Pregabalin Poisoning and Abuse Cases in Serbia: A Ten-Year Retrospective Study
VL  - 16
SP  - 1239
EP  - 1250
DO  - 10.2147/IJGM.S405616
ER  - 

@article{
author = "Antunović, Marko and Vučinić, Slavica and Kotur-Stevuljević, Jelena and Krstić, Kristijan and Jović-Stošić, Jasmina and Kilibarda, Vesna and Perković-Vukčević, Nataša and Đorđević, Snežana",
abstract = "Purpose: The aim of this study was to analyze data on gabapentinoid-related attendances to the National Poison Control Center of Serbia (NPCC), particularly abuse cases; to estimate its changes and to compare it with trends in national consumption rates of these drugs. We also aimed to analyze the main characteristics of the study population and to investigate the major clinical effects in poisoned patients. Patients and Methods: This is a retrospective study of patients admitted to the NPCC for acute poisoning involving gabapentinoids from 1 May 2012 to 1 October 2022. Results: There were 357 (95.5%) pregabalin-related and 17 (4.5%) gabapentin-related poisoning cases in 302 patients. Abuse of pregabalin was detected in 27.8% (84/302), while gabapentin abuse occurred in 0.7% (2/302) of all patients. A steady increase in rates of pregabalin poisoning and abuse cases strongly correlated with the increase in overall consumption of this drug, while there were no significant changes in rates of gabapentin consumption, poisoning and abuse rate during the study period. Most patients who abused pregabalin pregabalin were males (84.5%) and the median age was 26 years (range: 15–45 years). Almost 60% of patients who abused pregabalin (48/84) belonged to the migrant population. Co-ingestions occurred in 89.4% of pregabalin-related cases (319/357), resulting in more severe poisoning. The most often co-ingested drugs were benzodiazepines and among them clonazepam was detected in the largest number of cases. Conclusion: The poisoning and abuse cases involving pregabalin are on the rise in Serbia, which coincided with an increase in its overall consumption during the study period. Isolated pregabalin ingestions resulted in mild poisoning, although severe symptoms such as coma and bradycardia were recorded. When prescribing pregabalin to patients at risk of abuse caution is needed. Strengthening the measures for dispensing of pregabalin may reduce the risks associated with its abuse.",
publisher = "Dove Medical Press Ltd",
journal = "International Journal of General Medicine",
title = "Rise of Pregabalin Poisoning and Abuse Cases in Serbia: A Ten-Year Retrospective Study",
volume = "16",
pages = "1239-1250",
doi = "10.2147/IJGM.S405616"
}

Antunović, M., Vučinić, S., Kotur-Stevuljević, J., Krstić, K., Jović-Stošić, J., Kilibarda, V., Perković-Vukčević, N.,& Đorđević, S..Rise of Pregabalin Poisoning and Abuse Cases in Serbia: A Ten-Year Retrospective Study. in International Journal of General Medicine
Dove Medical Press Ltd., 16, 1239-1250.
https://doi.org/10.2147/IJGM.S405616

Antunović M, Vučinić S, Kotur-Stevuljević J, Krstić K, Jović-Stošić J, Kilibarda V, Perković-Vukčević N, Đorđević S. Rise of Pregabalin Poisoning and Abuse Cases in Serbia: A Ten-Year Retrospective Study. in International Journal of General Medicine.16:1239-1250.
doi:10.2147/IJGM.S405616 .

Antunović, Marko, Vučinić, Slavica, Kotur-Stevuljević, Jelena, Krstić, Kristijan, Jović-Stošić, Jasmina, Kilibarda, Vesna, Perković-Vukčević, Nataša, Đorđević, Snežana, "Rise of Pregabalin Poisoning and Abuse Cases in Serbia: A Ten-Year Retrospective Study" in International Journal of General Medicine, 16:1239-1250,
https://doi.org/10.2147/IJGM.S405616 . .

TY  - CONF
AU  - Popović Nikolić, Marija
AU  - Popović, Gordana
AU  - Oljačić, Slavica
AU  - Nikolić, Katarina
PY  - 2023-09
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5045
AB  - The protolytic equilibria of bilastine were studied experimentally and theoretically. The
pKa values were determined potentiometrically at a constant ionic strength (0.1 M NaCl) and
temperature 25 °C. Energy calculation of the optimized structures of the equilibrium forms was
performed at the B3LYP/6-31G (d,p) level of the Density Functional Theory (DFT). The results of
the theoretical study helped to define the ionization profile of bilastine and to assign the
experimentally determined pKa values to the corresponding ionizable groups.
PB  - Institute for Information Technologies, University of Kragujevac, Serbia
C3  - 2nd International Conference on Chemo and Bioinformatics ICCBIKG 2023, BOOK OF PROCEEDINGS
T1  - Theoretical and experimental study of bilastine ionization
SP  - 427
EP  - 430
DO  - 10.46793/ICCBI23.427PN
ER  - 

@conference{
author = "Popović Nikolić, Marija and Popović, Gordana and Oljačić, Slavica and Nikolić, Katarina",
year = "2023-09",
abstract = "The protolytic equilibria of bilastine were studied experimentally and theoretically. The
pKa values were determined potentiometrically at a constant ionic strength (0.1 M NaCl) and
temperature 25 °C. Energy calculation of the optimized structures of the equilibrium forms was
performed at the B3LYP/6-31G (d,p) level of the Density Functional Theory (DFT). The results of
the theoretical study helped to define the ionization profile of bilastine and to assign the
experimentally determined pKa values to the corresponding ionizable groups.",
publisher = "Institute for Information Technologies, University of Kragujevac, Serbia",
journal = "2nd International Conference on Chemo and Bioinformatics ICCBIKG 2023, BOOK OF PROCEEDINGS",
title = "Theoretical and experimental study of bilastine ionization",
pages = "427-430",
doi = "10.46793/ICCBI23.427PN"
}

Popović Nikolić, M., Popović, G., Oljačić, S.,& Nikolić, K.. (2023-09). Theoretical and experimental study of bilastine ionization. in 2nd International Conference on Chemo and Bioinformatics ICCBIKG 2023, BOOK OF PROCEEDINGS
Institute for Information Technologies, University of Kragujevac, Serbia., 427-430.
https://doi.org/10.46793/ICCBI23.427PN

Popović Nikolić M, Popović G, Oljačić S, Nikolić K. Theoretical and experimental study of bilastine ionization. in 2nd International Conference on Chemo and Bioinformatics ICCBIKG 2023, BOOK OF PROCEEDINGS. 2023;:427-430.
doi:10.46793/ICCBI23.427PN .

Popović Nikolić, Marija, Popović, Gordana, Oljačić, Slavica, Nikolić, Katarina, "Theoretical and experimental study of bilastine ionization" in 2nd International Conference on Chemo and Bioinformatics ICCBIKG 2023, BOOK OF PROCEEDINGS (2023-09):427-430,
https://doi.org/10.46793/ICCBI23.427PN . .

TY  - CONF
AU  - Beljkaš, Milan
AU  - Rebić, Jelena
AU  - Radan, Milica
AU  - Đikić, Teodora
AU  - Oljacic, Slavica
AU  - Nikolić, Katarina
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5043
AB  - Rho-associated coiled-coil kinases (ROCKs) are involved in essential
cellular functions such as adhesion, contraction, motility, proliferation, and cell
survival/apoptosis. Four ROCK inhibitors have already been approved by the FDA and
are used to treat glaucoma (ripasudil and netarsudil), cerebral vasospasm (fasudil), and
graft-versus-host disease (belumosudil). Recent studies have focused on exploring the
role of ROCK kinase inhibitors in cancer treatment and the development of new ROCK
inhibitors. The main objective of this study was to identify critical structural features
relevant to the inhibition of ROCK1 using a ligand-based 3D-QSAR (3D quantitative
structure-activity relationship) method. The 3D-QSAR model for ROCK1 was created
and validated using internal and external validation parameters (R2, Q2, R2pred, rm2, r/2m, 𝑟���������𝑚���������
2̅̅̅
and ∆r2m). The main structural features that correlate with the inhibition of ROCK1 were
identified (e.g., heterocycle with hydrogen donor group like nitrogen atom) and further
structural modifications of the ROCK1 inhibitors that contribute to increased activity
were proposed (removal of the amino group of the oxadiazole, modification of the
substituents of the phenyl ring).
PB  - Institute for Information Technologies, University of Kragujevac, Serbia
C3  - 2nd International Conference on Chemo and Bioinformatics, BOOK OF PROOCEEDINGS
T1  - 3D-Quantitative Structure-Activity Relationship and design of novel Rho- associated protein kinases-1 (ROCK1) inhibitors
SP  - 1584
EP  - 1588
DO  - 10.46793/ICCBI23.584B
ER  - 

@conference{
author = "Beljkaš, Milan and Rebić, Jelena and Radan, Milica and Đikić, Teodora and Oljacic, Slavica and Nikolić, Katarina",
year = "2023",
abstract = "Rho-associated coiled-coil kinases (ROCKs) are involved in essential
cellular functions such as adhesion, contraction, motility, proliferation, and cell
survival/apoptosis. Four ROCK inhibitors have already been approved by the FDA and
are used to treat glaucoma (ripasudil and netarsudil), cerebral vasospasm (fasudil), and
graft-versus-host disease (belumosudil). Recent studies have focused on exploring the
role of ROCK kinase inhibitors in cancer treatment and the development of new ROCK
inhibitors. The main objective of this study was to identify critical structural features
relevant to the inhibition of ROCK1 using a ligand-based 3D-QSAR (3D quantitative
structure-activity relationship) method. The 3D-QSAR model for ROCK1 was created
and validated using internal and external validation parameters (R2, Q2, R2pred, rm2, r/2m, 𝑟���������𝑚���������
2̅̅̅
and ∆r2m). The main structural features that correlate with the inhibition of ROCK1 were
identified (e.g., heterocycle with hydrogen donor group like nitrogen atom) and further
structural modifications of the ROCK1 inhibitors that contribute to increased activity
were proposed (removal of the amino group of the oxadiazole, modification of the
substituents of the phenyl ring).",
publisher = "Institute for Information Technologies, University of Kragujevac, Serbia",
journal = "2nd International Conference on Chemo and Bioinformatics, BOOK OF PROOCEEDINGS",
title = "3D-Quantitative Structure-Activity Relationship and design of novel Rho- associated protein kinases-1 (ROCK1) inhibitors",
pages = "1584-1588",
doi = "10.46793/ICCBI23.584B"
}

Beljkaš, M., Rebić, J., Radan, M., Đikić, T., Oljacic, S.,& Nikolić, K.. (2023). 3D-Quantitative Structure-Activity Relationship and design of novel Rho- associated protein kinases-1 (ROCK1) inhibitors. in 2nd International Conference on Chemo and Bioinformatics, BOOK OF PROOCEEDINGS
Institute for Information Technologies, University of Kragujevac, Serbia., 1584-1588.
https://doi.org/10.46793/ICCBI23.584B

Beljkaš M, Rebić J, Radan M, Đikić T, Oljacic S, Nikolić K. 3D-Quantitative Structure-Activity Relationship and design of novel Rho- associated protein kinases-1 (ROCK1) inhibitors. in 2nd International Conference on Chemo and Bioinformatics, BOOK OF PROOCEEDINGS. 2023;:1584-1588.
doi:10.46793/ICCBI23.584B .

Beljkaš, Milan, Rebić, Jelena, Radan, Milica, Đikić, Teodora, Oljacic, Slavica, Nikolić, Katarina, "3D-Quantitative Structure-Activity Relationship and design of novel Rho- associated protein kinases-1 (ROCK1) inhibitors" in 2nd International Conference on Chemo and Bioinformatics, BOOK OF PROOCEEDINGS (2023):1584-1588,
https://doi.org/10.46793/ICCBI23.584B . .

TY  - CONF
AU  - Beljkaš, Milan
AU  - Rebić, Jelena
AU  - Radan, Milica
AU  - Đikić, Teodora
AU  - Oljacic, Slavica
AU  - Nikolić, Katarina
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5041
AB  - Overexpression of Rho-associated protein kinases has been associated with various
diseases, including tumors. None of the approved ROCK inhibitors are used for cancer treatment.
However, some of them have been shown to have anti-tumor potential. The main objective of this
study was to develop novel ROCK1 inhibitors using the structure-based method, molecular
docking, and prediction of pharmacokinetic properties using the ADMET predictor. The key
interactions that strongly correlate with the activity of ROCK1 inhibitors are hydrogen bonds
between amino acid residues Met156, Glu154 and the hinge region of the inhibitors, indicating
possible structural changes in the hinge region of studied compounds. On the other hand, the
lack of interactions between 1,3-benzoxadiol moiety and the enzyme presents a promising
approach for further structural modifications in order to design more effective ROCK1 inhibitors.
All the important interactions between the developed ROCK1 inhibitors and the binding site
of the enzyme were established. They also showed acceptable pharmacokinetic properties and
could be further used for synthesis and evaluation by various biological assays.
PB  - Institute for Information Technologies, University of Kragujevac, Serbia
C3  - 2nd International Conference on Chemo and Bioinformatics ICCBIKG 2023, BOOK OF PROCEEDINGS
T1  - Virtual Docking, design and in silico ADMET profiling of novel Rho- associated protein kinases-1 (ROCK1) inhibitors
SP  - 1589
EP  - 1592
DO  - 10.46793/ICCBI23.589B
ER  - 

@conference{
author = "Beljkaš, Milan and Rebić, Jelena and Radan, Milica and Đikić, Teodora and Oljacic, Slavica and Nikolić, Katarina",
year = "2023",
abstract = "Overexpression of Rho-associated protein kinases has been associated with various
diseases, including tumors. None of the approved ROCK inhibitors are used for cancer treatment.
However, some of them have been shown to have anti-tumor potential. The main objective of this
study was to develop novel ROCK1 inhibitors using the structure-based method, molecular
docking, and prediction of pharmacokinetic properties using the ADMET predictor. The key
interactions that strongly correlate with the activity of ROCK1 inhibitors are hydrogen bonds
between amino acid residues Met156, Glu154 and the hinge region of the inhibitors, indicating
possible structural changes in the hinge region of studied compounds. On the other hand, the
lack of interactions between 1,3-benzoxadiol moiety and the enzyme presents a promising
approach for further structural modifications in order to design more effective ROCK1 inhibitors.
All the important interactions between the developed ROCK1 inhibitors and the binding site
of the enzyme were established. They also showed acceptable pharmacokinetic properties and
could be further used for synthesis and evaluation by various biological assays.",
publisher = "Institute for Information Technologies, University of Kragujevac, Serbia",
journal = "2nd International Conference on Chemo and Bioinformatics ICCBIKG 2023, BOOK OF PROCEEDINGS",
title = "Virtual Docking, design and in silico ADMET profiling of novel Rho- associated protein kinases-1 (ROCK1) inhibitors",
pages = "1589-1592",
doi = "10.46793/ICCBI23.589B"
}

Beljkaš, M., Rebić, J., Radan, M., Đikić, T., Oljacic, S.,& Nikolić, K.. (2023). Virtual Docking, design and in silico ADMET profiling of novel Rho- associated protein kinases-1 (ROCK1) inhibitors. in 2nd International Conference on Chemo and Bioinformatics ICCBIKG 2023, BOOK OF PROCEEDINGS
Institute for Information Technologies, University of Kragujevac, Serbia., 1589-1592.
https://doi.org/10.46793/ICCBI23.589B

Beljkaš M, Rebić J, Radan M, Đikić T, Oljacic S, Nikolić K. Virtual Docking, design and in silico ADMET profiling of novel Rho- associated protein kinases-1 (ROCK1) inhibitors. in 2nd International Conference on Chemo and Bioinformatics ICCBIKG 2023, BOOK OF PROCEEDINGS. 2023;:1589-1592.
doi:10.46793/ICCBI23.589B .

Beljkaš, Milan, Rebić, Jelena, Radan, Milica, Đikić, Teodora, Oljacic, Slavica, Nikolić, Katarina, "Virtual Docking, design and in silico ADMET profiling of novel Rho- associated protein kinases-1 (ROCK1) inhibitors" in 2nd International Conference on Chemo and Bioinformatics ICCBIKG 2023, BOOK OF PROCEEDINGS (2023):1589-1592,
https://doi.org/10.46793/ICCBI23.589B . .

TY  - CONF
AU  - Popović-Nikolić, Marija
AU  - Oljačić, Slavica
AU  - Popović, Gordana
AU  - Nikolić, Katarina
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5013
PB  - European Research Network on Signal Transduction CA18133
C3  - 3rd Transatlantic ECI GPCR Symposium - Early Career Investigators – September 7-8, 2023, Online event
T1  - The ionization of a cysteinyl leukotriene receptor antagonist, montelukast, in the presence of biomembrane mimetic systems
SP  - 35
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5013
ER  - 

@conference{
author = "Popović-Nikolić, Marija and Oljačić, Slavica and Popović, Gordana and Nikolić, Katarina",
year = "2023",
publisher = "European Research Network on Signal Transduction CA18133",
journal = "3rd Transatlantic ECI GPCR Symposium - Early Career Investigators – September 7-8, 2023, Online event",
title = "The ionization of a cysteinyl leukotriene receptor antagonist, montelukast, in the presence of biomembrane mimetic systems",
pages = "35",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5013"
}

Popović-Nikolić, M., Oljačić, S., Popović, G.,& Nikolić, K.. (2023). The ionization of a cysteinyl leukotriene receptor antagonist, montelukast, in the presence of biomembrane mimetic systems. in 3rd Transatlantic ECI GPCR Symposium - Early Career Investigators – September 7-8, 2023, Online event
European Research Network on Signal Transduction CA18133., 35.
https://hdl.handle.net/21.15107/rcub_farfar_5013

Popović-Nikolić M, Oljačić S, Popović G, Nikolić K. The ionization of a cysteinyl leukotriene receptor antagonist, montelukast, in the presence of biomembrane mimetic systems. in 3rd Transatlantic ECI GPCR Symposium - Early Career Investigators – September 7-8, 2023, Online event. 2023;:35.
https://hdl.handle.net/21.15107/rcub_farfar_5013 .

Popović-Nikolić, Marija, Oljačić, Slavica, Popović, Gordana, Nikolić, Katarina, "The ionization of a cysteinyl leukotriene receptor antagonist, montelukast, in the presence of biomembrane mimetic systems" in 3rd Transatlantic ECI GPCR Symposium - Early Career Investigators – September 7-8, 2023, Online event (2023):35,
https://hdl.handle.net/21.15107/rcub_farfar_5013 .

TY  - CONF
AU  - Beljkaš, Milan
AU  - Petković, Miloš
AU  - Nikolić, Katarina
AU  - Oljačić, Slavica
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5005
AB  - Histone deacetylases (HDACs) belong to a family of epigenetic enzymes that has 18 different
isoforms and play an important role in the development and progression of various tumors.
To date, five histone deacetylase inhibitors have been approved by the FDA, and are used
to treat multiple myeloma, cutaneous T-cell lymphoma, peripheral T-cell lymphoma, and
breast cancer (estrogen and/or progesterone positive) [1]. All of them are non-selective.
Therefore, their safety profile is poor and their efficacy is low in single therapy. One of our
previous research projects demonstrated the synergistic effect of HDAC inhibitors and
inhibitors of Rho-associated protein kinases (ROCK) in the treatment of pancreatic ductal
adenocarcinoma (PDAC) [2]. This finding led us to design of the dual HDAC/ROCK inhibitors
with potential effects on PDAC by using structure-based molecular docking method.
Molecular docking study was performed using GOLD software. The crystal structures of
ROCK1 (PDB: 6E9W), ROCK2 (PDB: 7JNT), HDAC1 (PDB: 5ICN), and HDAC6 (PDB: 5EDU)
enzymes were downloaded from the Protein Data Bank (PDB). The enzymes were prepared
for docking study using the online software Play Molecule-ProteinPrepare. The structures
of the ROCK1, ROCK2, HDAC1 and HDAC6 inhibitors with their pIC50 values were obtained
from the ChEMBL database. The dominant microspecies of all compounds at physiological
pH were selected by Marvin Sketch Sketch 6.1.0 program and their further geometrical
optimization were performed using the PM3 semi-empirical method and the Hartree-Fock
method with 3-21G basis set.
The virtual docking procedures for all four enzymes were validated and the calculated RMSD
values were below 2Å. The critical parts of the structures that establish the interactions
crucial for the inhibition of HDAC1, HDAC6, ROCK1, and ROCK2 were identified. Based on
the obtained resultsdual HDAC/ROCK inhibitors were designed and evaluated by validated
docking procedures and in silico ADMET profiling.
Taking into account all these findings, the most active compounds are selected and will be
further synthesized and evaluated using in vitro enzyme and cell tests.
PB  - International Association of Physical Chemists
C3  - 10th IAPC Meeting Tenth World Conference on Physico-Chemical Methods in Drug Discovery & Sixth World Conference on ADMET and DMPK Belgrade, Serbia, September 4-6
T1  - Virtual docking and design of novel Histone deacetylase and Rho- associated protein kinases dual inhibitors (HDAC/ROCKs)
SP  - 46
EP  - 46
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5005
ER  - 

@conference{
author = "Beljkaš, Milan and Petković, Miloš and Nikolić, Katarina and Oljačić, Slavica",
year = "2023",
abstract = "Histone deacetylases (HDACs) belong to a family of epigenetic enzymes that has 18 different
isoforms and play an important role in the development and progression of various tumors.
To date, five histone deacetylase inhibitors have been approved by the FDA, and are used
to treat multiple myeloma, cutaneous T-cell lymphoma, peripheral T-cell lymphoma, and
breast cancer (estrogen and/or progesterone positive) [1]. All of them are non-selective.
Therefore, their safety profile is poor and their efficacy is low in single therapy. One of our
previous research projects demonstrated the synergistic effect of HDAC inhibitors and
inhibitors of Rho-associated protein kinases (ROCK) in the treatment of pancreatic ductal
adenocarcinoma (PDAC) [2]. This finding led us to design of the dual HDAC/ROCK inhibitors
with potential effects on PDAC by using structure-based molecular docking method.
Molecular docking study was performed using GOLD software. The crystal structures of
ROCK1 (PDB: 6E9W), ROCK2 (PDB: 7JNT), HDAC1 (PDB: 5ICN), and HDAC6 (PDB: 5EDU)
enzymes were downloaded from the Protein Data Bank (PDB). The enzymes were prepared
for docking study using the online software Play Molecule-ProteinPrepare. The structures
of the ROCK1, ROCK2, HDAC1 and HDAC6 inhibitors with their pIC50 values were obtained
from the ChEMBL database. The dominant microspecies of all compounds at physiological
pH were selected by Marvin Sketch Sketch 6.1.0 program and their further geometrical
optimization were performed using the PM3 semi-empirical method and the Hartree-Fock
method with 3-21G basis set.
The virtual docking procedures for all four enzymes were validated and the calculated RMSD
values were below 2Å. The critical parts of the structures that establish the interactions
crucial for the inhibition of HDAC1, HDAC6, ROCK1, and ROCK2 were identified. Based on
the obtained resultsdual HDAC/ROCK inhibitors were designed and evaluated by validated
docking procedures and in silico ADMET profiling.
Taking into account all these findings, the most active compounds are selected and will be
further synthesized and evaluated using in vitro enzyme and cell tests.",
publisher = "International Association of Physical Chemists",
journal = "10th IAPC Meeting Tenth World Conference on Physico-Chemical Methods in Drug Discovery & Sixth World Conference on ADMET and DMPK Belgrade, Serbia, September 4-6",
title = "Virtual docking and design of novel Histone deacetylase and Rho- associated protein kinases dual inhibitors (HDAC/ROCKs)",
pages = "46-46",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5005"
}

Beljkaš, M., Petković, M., Nikolić, K.,& Oljačić, S.. (2023). Virtual docking and design of novel Histone deacetylase and Rho- associated protein kinases dual inhibitors (HDAC/ROCKs). in 10th IAPC Meeting Tenth World Conference on Physico-Chemical Methods in Drug Discovery & Sixth World Conference on ADMET and DMPK Belgrade, Serbia, September 4-6
International Association of Physical Chemists., 46-46.
https://hdl.handle.net/21.15107/rcub_farfar_5005

Beljkaš M, Petković M, Nikolić K, Oljačić S. Virtual docking and design of novel Histone deacetylase and Rho- associated protein kinases dual inhibitors (HDAC/ROCKs). in 10th IAPC Meeting Tenth World Conference on Physico-Chemical Methods in Drug Discovery & Sixth World Conference on ADMET and DMPK Belgrade, Serbia, September 4-6. 2023;:46-46.
https://hdl.handle.net/21.15107/rcub_farfar_5005 .

Beljkaš, Milan, Petković, Miloš, Nikolić, Katarina, Oljačić, Slavica, "Virtual docking and design of novel Histone deacetylase and Rho- associated protein kinases dual inhibitors (HDAC/ROCKs)" in 10th IAPC Meeting Tenth World Conference on Physico-Chemical Methods in Drug Discovery & Sixth World Conference on ADMET and DMPK Belgrade, Serbia, September 4-6 (2023):46-46,
https://hdl.handle.net/21.15107/rcub_farfar_5005 .

TY  - CONF
AU  - Popović-Nikolić, Marija
AU  - Oljačić, Slavica
AU  - Nikolić, Katarina
AU  - Popović, Gordana
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5004
AB  - Montelukast is a leukotriene receptor antagonist indicated for asthma prophylaxis in adults
as well as in pediatric patients 6 months of age and older. Because it is associated with
numerous side effects, including neuropsychiatric events, it is very important to monitor its
pharmacologic behavior when administered chronically. To gain better insight into the
pharmacological properties of ionizable drugs, their physicochemical properties should be
studied under conditions more similar to physiological, such as micellar solutions of
surfactants as biomembrane mimetic systems. Montelukast is an ampholyte with one acidic
(carboxyl) and one basic (quinoline nitrogen) group. In this study the effects of micellar
solutions of differently charged surfactants (anionic SDS, cationic CTAB, and nonionic TX-
100) on protolytic equilibria of montelukast were investigated potentiometrically. Solutions were titrated with standard NaOH solution (0.1017 M) at a constant ionic strength (0.1 M NaCl) and a temperature 25°C. Experimental data were analyzed using the Hyperquad program. Due to poor water solubility, the pKa values defining the ionization in water (pKa1 =4.07, pKa2 = 5.49), were obtained indirectly by extrapolation from the pKa* values determined potentiometrically in the different methanol-water mixtures (40%, 50%, and 55% wt/wt). The pKa values in 0.01M micellar solutions were determined without the use of cosolvent. Micelles contributed to the shift in protolytic equilibria of montelukast, anionic ΔpKa up to +1.20, cationic ΔpKa up to +0.27, and nonionic ΔpKa up to +0.98. More pronounced effects are observed on the ionization of carboxyl group than quinoline nitrogen. A change in the distribution of equilibrium forms in a relation to pure water, can be expected in physiological conditions, in interactions of montelukast with polar or charged biomolecules.
PB  - International Association of Physical Chemists
C3  - 10th IAPC Meeting Tenth World Conference on Physico-Chemical Methods in Drug Discovery & Sixth World Conference on ADMET and DMPK Belgrade, Serbia, September 4-6
T1  - Study of ionization of montelukast in differently charged micellar solutions as biomembrane mimetic systems
SP  - 49
EP  - 49
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5004
ER  - 

@conference{
author = "Popović-Nikolić, Marija and Oljačić, Slavica and Nikolić, Katarina and Popović, Gordana",
year = "2023",
abstract = "Montelukast is a leukotriene receptor antagonist indicated for asthma prophylaxis in adults
as well as in pediatric patients 6 months of age and older. Because it is associated with
numerous side effects, including neuropsychiatric events, it is very important to monitor its
pharmacologic behavior when administered chronically. To gain better insight into the
pharmacological properties of ionizable drugs, their physicochemical properties should be
studied under conditions more similar to physiological, such as micellar solutions of
surfactants as biomembrane mimetic systems. Montelukast is an ampholyte with one acidic
(carboxyl) and one basic (quinoline nitrogen) group. In this study the effects of micellar
solutions of differently charged surfactants (anionic SDS, cationic CTAB, and nonionic TX-
100) on protolytic equilibria of montelukast were investigated potentiometrically. Solutions were titrated with standard NaOH solution (0.1017 M) at a constant ionic strength (0.1 M NaCl) and a temperature 25°C. Experimental data were analyzed using the Hyperquad program. Due to poor water solubility, the pKa values defining the ionization in water (pKa1 =4.07, pKa2 = 5.49), were obtained indirectly by extrapolation from the pKa* values determined potentiometrically in the different methanol-water mixtures (40%, 50%, and 55% wt/wt). The pKa values in 0.01M micellar solutions were determined without the use of cosolvent. Micelles contributed to the shift in protolytic equilibria of montelukast, anionic ΔpKa up to +1.20, cationic ΔpKa up to +0.27, and nonionic ΔpKa up to +0.98. More pronounced effects are observed on the ionization of carboxyl group than quinoline nitrogen. A change in the distribution of equilibrium forms in a relation to pure water, can be expected in physiological conditions, in interactions of montelukast with polar or charged biomolecules.",
publisher = "International Association of Physical Chemists",
journal = "10th IAPC Meeting Tenth World Conference on Physico-Chemical Methods in Drug Discovery & Sixth World Conference on ADMET and DMPK Belgrade, Serbia, September 4-6",
title = "Study of ionization of montelukast in differently charged micellar solutions as biomembrane mimetic systems",
pages = "49-49",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5004"
}

Popović-Nikolić, M., Oljačić, S., Nikolić, K.,& Popović, G.. (2023). Study of ionization of montelukast in differently charged micellar solutions as biomembrane mimetic systems. in 10th IAPC Meeting Tenth World Conference on Physico-Chemical Methods in Drug Discovery & Sixth World Conference on ADMET and DMPK Belgrade, Serbia, September 4-6
International Association of Physical Chemists., 49-49.
https://hdl.handle.net/21.15107/rcub_farfar_5004

Popović-Nikolić M, Oljačić S, Nikolić K, Popović G. Study of ionization of montelukast in differently charged micellar solutions as biomembrane mimetic systems. in 10th IAPC Meeting Tenth World Conference on Physico-Chemical Methods in Drug Discovery & Sixth World Conference on ADMET and DMPK Belgrade, Serbia, September 4-6. 2023;:49-49.
https://hdl.handle.net/21.15107/rcub_farfar_5004 .

Popović-Nikolić, Marija, Oljačić, Slavica, Nikolić, Katarina, Popović, Gordana, "Study of ionization of montelukast in differently charged micellar solutions as biomembrane mimetic systems" in 10th IAPC Meeting Tenth World Conference on Physico-Chemical Methods in Drug Discovery & Sixth World Conference on ADMET and DMPK Belgrade, Serbia, September 4-6 (2023):49-49,
https://hdl.handle.net/21.15107/rcub_farfar_5004 .

TY  - CONF
AU  - Popović Nikolić, Marija
AU  - Nikolić, Katarina
AU  - Aleksić, Mara
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5044
AB  - he redox properties of two quinoxaline derivatives, brimonidine and varenicline,
previously studied electrochemically, were evaluated by performing a computational study. On
the basis of some useful quantum chemical parameters the differences and similarities between
their redox features were explained. The obtained results support the experimental findings that
the redox processes of both compounds are under strong influence of the solution pH, whereas
the reduction of brimonidine occurs easier than the reduction of varenicline, at corresponding pH
values.
PB  - Institute for Information Technologies, University of Kragujevac, Serbia
C3  - 2nd International Conference on Chemo and Bioinformatics ICCBIKG 2023, BOOK OF PROCEEDINGS
T1  - DFT approach of the redox properties of brimonidine and varenicline
SP  - 423
EP  - 426
DO  - 10.46793/ICCBI23.423PN
ER  - 

@conference{
author = "Popović Nikolić, Marija and Nikolić, Katarina and Aleksić, Mara",
year = "2023",
abstract = "he redox properties of two quinoxaline derivatives, brimonidine and varenicline,
previously studied electrochemically, were evaluated by performing a computational study. On
the basis of some useful quantum chemical parameters the differences and similarities between
their redox features were explained. The obtained results support the experimental findings that
the redox processes of both compounds are under strong influence of the solution pH, whereas
the reduction of brimonidine occurs easier than the reduction of varenicline, at corresponding pH
values.",
publisher = "Institute for Information Technologies, University of Kragujevac, Serbia",
journal = "2nd International Conference on Chemo and Bioinformatics ICCBIKG 2023, BOOK OF PROCEEDINGS",
title = "DFT approach of the redox properties of brimonidine and varenicline",
pages = "423-426",
doi = "10.46793/ICCBI23.423PN"
}

Popović Nikolić, M., Nikolić, K.,& Aleksić, M.. (2023). DFT approach of the redox properties of brimonidine and varenicline. in 2nd International Conference on Chemo and Bioinformatics ICCBIKG 2023, BOOK OF PROCEEDINGS
Institute for Information Technologies, University of Kragujevac, Serbia., 423-426.
https://doi.org/10.46793/ICCBI23.423PN

Popović Nikolić M, Nikolić K, Aleksić M. DFT approach of the redox properties of brimonidine and varenicline. in 2nd International Conference on Chemo and Bioinformatics ICCBIKG 2023, BOOK OF PROCEEDINGS. 2023;:423-426.
doi:10.46793/ICCBI23.423PN .

Popović Nikolić, Marija, Nikolić, Katarina, Aleksić, Mara, "DFT approach of the redox properties of brimonidine and varenicline" in 2nd International Conference on Chemo and Bioinformatics ICCBIKG 2023, BOOK OF PROCEEDINGS (2023):423-426,
https://doi.org/10.46793/ICCBI23.423PN . .

TY  - CONF
AU  - Obradović, Darija
AU  - Komsta, Lukasz
AU  - Popović-Nikolić, Marija
AU  - Milutinović, Jovana
AU  - Lazović, Saša
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5002
AB  - Most biomimetic chromatography measurements provide information on the ability of
drugs to pass through cell membranes, their interaction with protein-based structures and
distribution properties. The biomimetic properties of thin-layer chromatography (TLC)
conditions have not been investigated so far. In our previous research, the presence of dual
retention mechanisms for selected imidazoline and serotonin receptor ligands was
confirmed under TLC conditions on C-18, diol, and a silica-based phases. The mobile phase
was amixture of ACN and water with 20 mM ammoniumacetate and 0.1 volume %of acetic
acid [1]. In this research, the average retention parameters were determined by using the
integration procedure [2]. The parameter RMH is the average retention in the hydrophilicdominated
(HILIC), while RMR is the average retention in the region of reversed-phase
interactions (RP). The parameter RMA corresponds to the average retention within the
overall HILIC/RP region. The lipophilicity successfully correlates with the C-18 and silica
based behaviour. For plasma protein binding affinity, the best correlations were found
within C-18 and silica-based systems (r > 0.70). There is also a correlation of average silica
gel and C-18 mechanism of interaction with the volume of distribution (r > 0.73), and the
intestinal absorption (r > 0.70). The retention behaviour on the diol phase showed a good
correlation with the P-gp inhibitor activity (r = 0.80). TLC systems that provide dual
retention mechanisms can be successfully used in the rapid biomimetic profiling of
serotonin and imidazoline receptor ligands in the first steps of drug discovery.
PB  - International Association of Physical Chemists
C3  - 10th IAPC Meeting Tenth World Conference on Physico-Chemical Methods in Drug Discovery & Sixth World Conference on ADMET and DMPK Belgrade, Serbia, September 4-6
T1  - Biomimetic characteristics of dual TLC retention mechanism
SP  - 48
EP  - 48
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5002
ER  - 

@conference{
author = "Obradović, Darija and Komsta, Lukasz and Popović-Nikolić, Marija and Milutinović, Jovana and Lazović, Saša",
year = "2023",
abstract = "Most biomimetic chromatography measurements provide information on the ability of
drugs to pass through cell membranes, their interaction with protein-based structures and
distribution properties. The biomimetic properties of thin-layer chromatography (TLC)
conditions have not been investigated so far. In our previous research, the presence of dual
retention mechanisms for selected imidazoline and serotonin receptor ligands was
confirmed under TLC conditions on C-18, diol, and a silica-based phases. The mobile phase
was amixture of ACN and water with 20 mM ammoniumacetate and 0.1 volume %of acetic
acid [1]. In this research, the average retention parameters were determined by using the
integration procedure [2]. The parameter RMH is the average retention in the hydrophilicdominated
(HILIC), while RMR is the average retention in the region of reversed-phase
interactions (RP). The parameter RMA corresponds to the average retention within the
overall HILIC/RP region. The lipophilicity successfully correlates with the C-18 and silica
based behaviour. For plasma protein binding affinity, the best correlations were found
within C-18 and silica-based systems (r > 0.70). There is also a correlation of average silica
gel and C-18 mechanism of interaction with the volume of distribution (r > 0.73), and the
intestinal absorption (r > 0.70). The retention behaviour on the diol phase showed a good
correlation with the P-gp inhibitor activity (r = 0.80). TLC systems that provide dual
retention mechanisms can be successfully used in the rapid biomimetic profiling of
serotonin and imidazoline receptor ligands in the first steps of drug discovery.",
publisher = "International Association of Physical Chemists",
journal = "10th IAPC Meeting Tenth World Conference on Physico-Chemical Methods in Drug Discovery & Sixth World Conference on ADMET and DMPK Belgrade, Serbia, September 4-6",
title = "Biomimetic characteristics of dual TLC retention mechanism",
pages = "48-48",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5002"
}

Obradović, D., Komsta, L., Popović-Nikolić, M., Milutinović, J.,& Lazović, S.. (2023). Biomimetic characteristics of dual TLC retention mechanism. in 10th IAPC Meeting Tenth World Conference on Physico-Chemical Methods in Drug Discovery & Sixth World Conference on ADMET and DMPK Belgrade, Serbia, September 4-6
International Association of Physical Chemists., 48-48.
https://hdl.handle.net/21.15107/rcub_farfar_5002

Obradović D, Komsta L, Popović-Nikolić M, Milutinović J, Lazović S. Biomimetic characteristics of dual TLC retention mechanism. in 10th IAPC Meeting Tenth World Conference on Physico-Chemical Methods in Drug Discovery & Sixth World Conference on ADMET and DMPK Belgrade, Serbia, September 4-6. 2023;:48-48.
https://hdl.handle.net/21.15107/rcub_farfar_5002 .

Obradović, Darija, Komsta, Lukasz, Popović-Nikolić, Marija, Milutinović, Jovana, Lazović, Saša, "Biomimetic characteristics of dual TLC retention mechanism" in 10th IAPC Meeting Tenth World Conference on Physico-Chemical Methods in Drug Discovery & Sixth World Conference on ADMET and DMPK Belgrade, Serbia, September 4-6 (2023):48-48,
https://hdl.handle.net/21.15107/rcub_farfar_5002 .

TY  - JOUR
AU  - Popović-Nikolić, Marija
AU  - Nikolić, Katarina
AU  - Popović, Gordana
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4687
AB  - The acid–base equilibria of six ACE inhibitors (ACEIs), captopril, cilazapril, enalapril, lisinopril, quinapril, and rami-
pril, were investigated in the presence of micelles of nonionic surfactant Brij 35. The pKa values were potentiometrically
determined at 25 °C and at a constant ionic strength (0.1 M NaCl). The obtained potentiometric data were evaluated in the
computer program Hyperquad. On the basis of the shift in the pKa values (ΔpKa ) determined in micellar media in relation
to the pKa values previously determined in “pure” water, the effect of Brij 35 micelles on ACEIs ionization was estimated.
The presence of nonionic Brij 35 micelles caused a shift in the pKa values of all ionizable groups of the investigated ACEIs
(ΔpKa from − 3.44 to + 1.9) while shifting the protolytic equilibria of both acidic and basic groups toward the molecular
form. The Brij 35 micelles expressed the most pronounced effect on the ionization of captopril among the investigated ACEIs
and stronger effect on the ionization of amino than on the ionization of carboxyl groups. The obtained results suggest that
ionizable functional groups of ACEIs are involved in interactions with palisade layer of nonionic Brij 35 micelles, which
potentially can be considered in physiological conditions. Distribution diagrams of the investigated ACEIs equilibrium
forms as a function of pH indicate that the change in distribution is most strongly expressed in pH range 4–8, which includes
biopharmaceutically important pH values.
PB  - Springer
T2  - Monatshefte für Chemie - Chemical Monthly
T1  - Protolytic equilibria of ACE inhibitors in micellar solution of nonionic surfactant Brij 35
VL  - 154
SP  - 615
EP  - 624
DO  - 10.1007/s00706-023-03059-2
ER  - 

@article{
author = "Popović-Nikolić, Marija and Nikolić, Katarina and Popović, Gordana",
year = "2023",
abstract = "The acid–base equilibria of six ACE inhibitors (ACEIs), captopril, cilazapril, enalapril, lisinopril, quinapril, and rami-
pril, were investigated in the presence of micelles of nonionic surfactant Brij 35. The pKa values were potentiometrically
determined at 25 °C and at a constant ionic strength (0.1 M NaCl). The obtained potentiometric data were evaluated in the
computer program Hyperquad. On the basis of the shift in the pKa values (ΔpKa ) determined in micellar media in relation
to the pKa values previously determined in “pure” water, the effect of Brij 35 micelles on ACEIs ionization was estimated.
The presence of nonionic Brij 35 micelles caused a shift in the pKa values of all ionizable groups of the investigated ACEIs
(ΔpKa from − 3.44 to + 1.9) while shifting the protolytic equilibria of both acidic and basic groups toward the molecular
form. The Brij 35 micelles expressed the most pronounced effect on the ionization of captopril among the investigated ACEIs
and stronger effect on the ionization of amino than on the ionization of carboxyl groups. The obtained results suggest that
ionizable functional groups of ACEIs are involved in interactions with palisade layer of nonionic Brij 35 micelles, which
potentially can be considered in physiological conditions. Distribution diagrams of the investigated ACEIs equilibrium
forms as a function of pH indicate that the change in distribution is most strongly expressed in pH range 4–8, which includes
biopharmaceutically important pH values.",
publisher = "Springer",
journal = "Monatshefte für Chemie - Chemical Monthly",
title = "Protolytic equilibria of ACE inhibitors in micellar solution of nonionic surfactant Brij 35",
volume = "154",
pages = "615-624",
doi = "10.1007/s00706-023-03059-2"
}

Popović-Nikolić, M., Nikolić, K.,& Popović, G.. (2023). Protolytic equilibria of ACE inhibitors in micellar solution of nonionic surfactant Brij 35. in Monatshefte für Chemie - Chemical Monthly
Springer., 154, 615-624.
https://doi.org/10.1007/s00706-023-03059-2

Popović-Nikolić M, Nikolić K, Popović G. Protolytic equilibria of ACE inhibitors in micellar solution of nonionic surfactant Brij 35. in Monatshefte für Chemie - Chemical Monthly. 2023;154:615-624.
doi:10.1007/s00706-023-03059-2 .

Popović-Nikolić, Marija, Nikolić, Katarina, Popović, Gordana, "Protolytic equilibria of ACE inhibitors in micellar solution of nonionic surfactant Brij 35" in Monatshefte für Chemie - Chemical Monthly, 154 (2023):615-624,
https://doi.org/10.1007/s00706-023-03059-2 . .

TY  - CONF
AU  - Jovanović, Aleksandar
AU  - Stefanović-Vojinović, Jelena
AU  - Petrović, Silvana
AU  - Drobac, Milica
AU  - Tadić, Ivana
AU  - Catić-Đorđević, Aleksandra
AU  - Kukić-Marković, Jelena
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5042
AB  - Background: Although the use of medicinal plants have been known since ancient times, an increased use of herbal products is evident in the last few decades, mainly as a part of self-care treatments. In addition to the positive health effects, the use of herbal products can cause certain side effects as well as interactions with conventional therapy, especially among elderly patients.
Purpose: To examine the awareness, habits and attitudes of elderly patients towards the use of herbal products in the Republic of Serbia.
Method: The quantitative, non-experimental research was conducted using a questionnaire in public pharmacies during February 2020. Patients older than 60 years were included in the study. The study was anonymous, and patients gave informed consent.
Results: The study involved 436 patients, of whom 62.4% were female. Most of the respondents live in the city (72.9%) and have a high school or lower education degree (61.9%). Most patients (32.3%) use herbal products daily, while only 12.1% rarely or never use them. Every fourth patient (24.5%) uses them for treatment, while 69.5% to improve their health. Although the majority of patients purchase herbal products in pharmacies and in health food stores (53.6% and 20.8%, respectively), a significant number (19.3%) collect medicinal plants and prepare herbal preparations themselves. The main sources of information about medicinal plants / herbal products are pharmacists (39.7%) and people from the immediate area (28.4%), followed by the media (18.2%) and physicians (11.1%). Every fourth patient (24.5%) uses herbal products for the treatment of some health issues, while 69.5% of patients use herbal products to improve their health. 48.9% of them believe that herbal products are safe, but with some side effects, while 35.8% believe herbal products are completely free of any side effects. Half of respondents (51.4%) believe that herbal products are as effective as or more effective than synthetic medicines. Less than one-third of patients (29.8%) indicated that they knew the difference between herbal medicinal products and herbal dietary supplements. Still, a third of patients (36.7%) needed additional explanations for the use of herbal products. 76.6% of patients stated that they always receive advice from a pharmacist when purchasing herbal products.
Conclusion: The survey conducted in this work showed that elderly patients use herbal products to a considerable extent, which means that it is necessary to educate elderly patients on this matter. Pharmacists should play a vital role in this process and actively discuss the characteristics and proper use of herbal products with patients, especially having in mind the high possibility of interactions with conventional medicines and occurrence of side effects. Since the quality of some herbal products sometimes could be questionable, especially non-regulated ones, patients should be encouraged to purchase them from pharmacies and consult with pharmacists.
PB  - The International Pharmaceutical Federation (FIP)
C3  - Pharmacy Education
T1  - Awareness of elderly patients on herbal products in Serbia
VL  - 23
IS  - 5
SP  - 29
EP  - 29
DO  - 10.46542/pe.2023.235.138
ER  - 

@conference{
author = "Jovanović, Aleksandar and Stefanović-Vojinović, Jelena and Petrović, Silvana and Drobac, Milica and Tadić, Ivana and Catić-Đorđević, Aleksandra and Kukić-Marković, Jelena",
year = "2023",
abstract = "Background: Although the use of medicinal plants have been known since ancient times, an increased use of herbal products is evident in the last few decades, mainly as a part of self-care treatments. In addition to the positive health effects, the use of herbal products can cause certain side effects as well as interactions with conventional therapy, especially among elderly patients.
Purpose: To examine the awareness, habits and attitudes of elderly patients towards the use of herbal products in the Republic of Serbia.
Method: The quantitative, non-experimental research was conducted using a questionnaire in public pharmacies during February 2020. Patients older than 60 years were included in the study. The study was anonymous, and patients gave informed consent.
Results: The study involved 436 patients, of whom 62.4% were female. Most of the respondents live in the city (72.9%) and have a high school or lower education degree (61.9%). Most patients (32.3%) use herbal products daily, while only 12.1% rarely or never use them. Every fourth patient (24.5%) uses them for treatment, while 69.5% to improve their health. Although the majority of patients purchase herbal products in pharmacies and in health food stores (53.6% and 20.8%, respectively), a significant number (19.3%) collect medicinal plants and prepare herbal preparations themselves. The main sources of information about medicinal plants / herbal products are pharmacists (39.7%) and people from the immediate area (28.4%), followed by the media (18.2%) and physicians (11.1%). Every fourth patient (24.5%) uses herbal products for the treatment of some health issues, while 69.5% of patients use herbal products to improve their health. 48.9% of them believe that herbal products are safe, but with some side effects, while 35.8% believe herbal products are completely free of any side effects. Half of respondents (51.4%) believe that herbal products are as effective as or more effective than synthetic medicines. Less than one-third of patients (29.8%) indicated that they knew the difference between herbal medicinal products and herbal dietary supplements. Still, a third of patients (36.7%) needed additional explanations for the use of herbal products. 76.6% of patients stated that they always receive advice from a pharmacist when purchasing herbal products.
Conclusion: The survey conducted in this work showed that elderly patients use herbal products to a considerable extent, which means that it is necessary to educate elderly patients on this matter. Pharmacists should play a vital role in this process and actively discuss the characteristics and proper use of herbal products with patients, especially having in mind the high possibility of interactions with conventional medicines and occurrence of side effects. Since the quality of some herbal products sometimes could be questionable, especially non-regulated ones, patients should be encouraged to purchase them from pharmacies and consult with pharmacists.",
publisher = "The International Pharmaceutical Federation (FIP)",
journal = "Pharmacy Education",
title = "Awareness of elderly patients on herbal products in Serbia",
volume = "23",
number = "5",
pages = "29-29",
doi = "10.46542/pe.2023.235.138"
}

Jovanović, A., Stefanović-Vojinović, J., Petrović, S., Drobac, M., Tadić, I., Catić-Đorđević, A.,& Kukić-Marković, J.. (2023). Awareness of elderly patients on herbal products in Serbia. in Pharmacy Education
The International Pharmaceutical Federation (FIP)., 23(5), 29-29.
https://doi.org/10.46542/pe.2023.235.138

Jovanović A, Stefanović-Vojinović J, Petrović S, Drobac M, Tadić I, Catić-Đorđević A, Kukić-Marković J. Awareness of elderly patients on herbal products in Serbia. in Pharmacy Education. 2023;23(5):29-29.
doi:10.46542/pe.2023.235.138 .

Jovanović, Aleksandar, Stefanović-Vojinović, Jelena, Petrović, Silvana, Drobac, Milica, Tadić, Ivana, Catić-Đorđević, Aleksandra, Kukić-Marković, Jelena, "Awareness of elderly patients on herbal products in Serbia" in Pharmacy Education, 23, no. 5 (2023):29-29,
https://doi.org/10.46542/pe.2023.235.138 . .

TY  - JOUR
AU  - Nastić, Katarina
AU  - Pecikoza, Uroš
AU  - Labudović-Borović, Milica
AU  - Kotur-Stevuljević, Jelena
AU  - Micov, Ana
AU  - Jovanović, Aleksandar
AU  - Tomić, Maja
AU  - Stepanović-Petrović, Radica
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5018
AB  - Osteoarthritis represents a leading cause of disability with limited treatment options. Furthermore, it is frequently accompanied by cardiovascular and cognitive disorders, which can be exacerbated by osteoarthritis or drugs used for its treatment. Here, we examined the behavioral and cardiac effects of the novel antidepressant vortioxetine in an osteoarthritis model, and compared them to duloxetine (an established osteoarthritis treatment). Osteoarthritis was induced in male and female rats with an intraarticular sodium-monoiodoacetate injection. Antidepressants were orally administered for 28 days following induction. During this period the acetone, burrowing and novel-object-recognition tests (NORT) were used to assess the effects of antidepressants on pain hypersensitivity (cold allodynia), animal well-being and cognitive performance, respectively. Following behavioral experiments, heart muscles were collected for assessment of redox status/histology. Antidepressant treatment dose-dependently reduced cold allodynia in rats with osteoarthritis. Duloxetine (but not vortioxetine) depressed burrowing behavior in osteoarthritic rats in a dose-related manner. Osteoarthritis induction reduced cognitive performance in NORT, which was dose-dependently alleviated by vortioxetine (duloxetine improved performance only in female rats). Furthermore, duloxetine (but not vortioxetine) increased oxidative stress parameters in the heart muscles of female (but not male) rats and induced histological changes in cardiomyocytes indicative of oxidative damage. Vortioxetine displayed comparable efficacy to duloxetine in reducing pain hypersensitivity. Furthermore, vortioxetine (unlike duloxetine) dose-dependently improved cognitive performance and had no adverse effect on burrowing behavior (animal surrogate of well-being) and cardiac redox status/histology. Our results indicate that vortioxetine could be a potential osteoarthritis treatment (with better characteristics compared to duloxetine).
PB  - Elsevier Masson
T2  - Biomedicine and Pharmacotherapy
T1  - The antidepressant drugs vortioxetine and duloxetine differentially and sex-dependently affect animal well-being, cognitive performance, cardiac redox status and histology in a model of osteoarthritis
VL  - 166
DO  - 10.1016/j.biopha.2023.115360
ER  - 

@article{
author = "Nastić, Katarina and Pecikoza, Uroš and Labudović-Borović, Milica and Kotur-Stevuljević, Jelena and Micov, Ana and Jovanović, Aleksandar and Tomić, Maja and Stepanović-Petrović, Radica",
year = "2023",
abstract = "Osteoarthritis represents a leading cause of disability with limited treatment options. Furthermore, it is frequently accompanied by cardiovascular and cognitive disorders, which can be exacerbated by osteoarthritis or drugs used for its treatment. Here, we examined the behavioral and cardiac effects of the novel antidepressant vortioxetine in an osteoarthritis model, and compared them to duloxetine (an established osteoarthritis treatment). Osteoarthritis was induced in male and female rats with an intraarticular sodium-monoiodoacetate injection. Antidepressants were orally administered for 28 days following induction. During this period the acetone, burrowing and novel-object-recognition tests (NORT) were used to assess the effects of antidepressants on pain hypersensitivity (cold allodynia), animal well-being and cognitive performance, respectively. Following behavioral experiments, heart muscles were collected for assessment of redox status/histology. Antidepressant treatment dose-dependently reduced cold allodynia in rats with osteoarthritis. Duloxetine (but not vortioxetine) depressed burrowing behavior in osteoarthritic rats in a dose-related manner. Osteoarthritis induction reduced cognitive performance in NORT, which was dose-dependently alleviated by vortioxetine (duloxetine improved performance only in female rats). Furthermore, duloxetine (but not vortioxetine) increased oxidative stress parameters in the heart muscles of female (but not male) rats and induced histological changes in cardiomyocytes indicative of oxidative damage. Vortioxetine displayed comparable efficacy to duloxetine in reducing pain hypersensitivity. Furthermore, vortioxetine (unlike duloxetine) dose-dependently improved cognitive performance and had no adverse effect on burrowing behavior (animal surrogate of well-being) and cardiac redox status/histology. Our results indicate that vortioxetine could be a potential osteoarthritis treatment (with better characteristics compared to duloxetine).",
publisher = "Elsevier Masson",
journal = "Biomedicine and Pharmacotherapy",
title = "The antidepressant drugs vortioxetine and duloxetine differentially and sex-dependently affect animal well-being, cognitive performance, cardiac redox status and histology in a model of osteoarthritis",
volume = "166",
doi = "10.1016/j.biopha.2023.115360"
}

Nastić, K., Pecikoza, U., Labudović-Borović, M., Kotur-Stevuljević, J., Micov, A., Jovanović, A., Tomić, M.,& Stepanović-Petrović, R.. (2023). The antidepressant drugs vortioxetine and duloxetine differentially and sex-dependently affect animal well-being, cognitive performance, cardiac redox status and histology in a model of osteoarthritis. in Biomedicine and Pharmacotherapy
Elsevier Masson., 166.
https://doi.org/10.1016/j.biopha.2023.115360

Nastić K, Pecikoza U, Labudović-Borović M, Kotur-Stevuljević J, Micov A, Jovanović A, Tomić M, Stepanović-Petrović R. The antidepressant drugs vortioxetine and duloxetine differentially and sex-dependently affect animal well-being, cognitive performance, cardiac redox status and histology in a model of osteoarthritis. in Biomedicine and Pharmacotherapy. 2023;166.
doi:10.1016/j.biopha.2023.115360 .

Nastić, Katarina, Pecikoza, Uroš, Labudović-Borović, Milica, Kotur-Stevuljević, Jelena, Micov, Ana, Jovanović, Aleksandar, Tomić, Maja, Stepanović-Petrović, Radica, "The antidepressant drugs vortioxetine and duloxetine differentially and sex-dependently affect animal well-being, cognitive performance, cardiac redox status and histology in a model of osteoarthritis" in Biomedicine and Pharmacotherapy, 166 (2023),
https://doi.org/10.1016/j.biopha.2023.115360 . .

TY  - JOUR
AU  - Panić, Bojana
AU  - Jovanović, Marija
AU  - Lukić, Vera
AU  - Vučićević, Katarina
AU  - Miljković, Branislava
AU  - Milovanović, Srđan
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5040
AB  - Purpose: The goal of the study was to examine clozapine (CLZ) and norclozapine (NCLZ) therapeutic drug monitoring (TDM) data and associated sources of pharmacokinetic variability, particularly the impact of valproic acid (VPA) use. Methods: This study included 126 patients with psychiatric disorders on mono- or co-therapy with CLZ. Patients’ data during routine TDM were collected retrospectively from clinical records. The descriptive and statistical analysis was computed using IBM SPSS Statistics software (version 22, NY, USA). Multiple linear regression, based on the last observations, was used to assess correlation between demographic characteristics, life habits and co-therapy with dose-corrected serum levels (C/D) of CLZ and NCLZ, as well as CLZ/NCLZ. Results: A total of 295 CLZ concentrations were measured in 126 patients, with a mean of 275.5 ± 174.4 µg/L, while 124 NCLZ concentrations were determined in 74 patients, with a mean of 194.6 ± 149.8 µg/L. A statistically significant effect on ln-transformed CLZ C/D was confirmed for sex and smoking, whereas sex, smoking and VPA therapy were associated with ln-transformed NCLZ C/D. According to the final models, lower values of NCLZ C/D for about 45.9% can be expected in patients receiving VPA. Concomitant use of VPA was the only factor detected to contribute in CLZ/NCLZ variability. Conclusion: The results of this study may help clinicians interpret TDM data and optimize CLZ dosing regimens, especially in patients concomitantly treated with VPA. Our results show that VPA primarily decreases NCLZ levels, while alteration of the parent drug is not statistically significant.
PB  - Springer
T2  - European Journal of Clinical Pharmacology
T1  - Association of clozapine and norclozapine levels with patient and therapy characteristics—focus on interaction with valproic acid
DO  - 10.1007/s00228-023-03569-2
ER  - 

@article{
author = "Panić, Bojana and Jovanović, Marija and Lukić, Vera and Vučićević, Katarina and Miljković, Branislava and Milovanović, Srđan",
year = "2023",
abstract = "Purpose: The goal of the study was to examine clozapine (CLZ) and norclozapine (NCLZ) therapeutic drug monitoring (TDM) data and associated sources of pharmacokinetic variability, particularly the impact of valproic acid (VPA) use. Methods: This study included 126 patients with psychiatric disorders on mono- or co-therapy with CLZ. Patients’ data during routine TDM were collected retrospectively from clinical records. The descriptive and statistical analysis was computed using IBM SPSS Statistics software (version 22, NY, USA). Multiple linear regression, based on the last observations, was used to assess correlation between demographic characteristics, life habits and co-therapy with dose-corrected serum levels (C/D) of CLZ and NCLZ, as well as CLZ/NCLZ. Results: A total of 295 CLZ concentrations were measured in 126 patients, with a mean of 275.5 ± 174.4 µg/L, while 124 NCLZ concentrations were determined in 74 patients, with a mean of 194.6 ± 149.8 µg/L. A statistically significant effect on ln-transformed CLZ C/D was confirmed for sex and smoking, whereas sex, smoking and VPA therapy were associated with ln-transformed NCLZ C/D. According to the final models, lower values of NCLZ C/D for about 45.9% can be expected in patients receiving VPA. Concomitant use of VPA was the only factor detected to contribute in CLZ/NCLZ variability. Conclusion: The results of this study may help clinicians interpret TDM data and optimize CLZ dosing regimens, especially in patients concomitantly treated with VPA. Our results show that VPA primarily decreases NCLZ levels, while alteration of the parent drug is not statistically significant.",
publisher = "Springer",
journal = "European Journal of Clinical Pharmacology",
title = "Association of clozapine and norclozapine levels with patient and therapy characteristics—focus on interaction with valproic acid",
doi = "10.1007/s00228-023-03569-2"
}

Panić, B., Jovanović, M., Lukić, V., Vučićević, K., Miljković, B.,& Milovanović, S.. (2023). Association of clozapine and norclozapine levels with patient and therapy characteristics—focus on interaction with valproic acid. in European Journal of Clinical Pharmacology
Springer..
https://doi.org/10.1007/s00228-023-03569-2

Panić B, Jovanović M, Lukić V, Vučićević K, Miljković B, Milovanović S. Association of clozapine and norclozapine levels with patient and therapy characteristics—focus on interaction with valproic acid. in European Journal of Clinical Pharmacology. 2023;.
doi:10.1007/s00228-023-03569-2 .

Panić, Bojana, Jovanović, Marija, Lukić, Vera, Vučićević, Katarina, Miljković, Branislava, Milovanović, Srđan, "Association of clozapine and norclozapine levels with patient and therapy characteristics—focus on interaction with valproic acid" in European Journal of Clinical Pharmacology (2023),
https://doi.org/10.1007/s00228-023-03569-2 . .

Jaćević, V., Dumanović, J., Grujić-Milanović, J., Milovanović, Z., Amidžić, L., Vojinović, N., Nežić, L., Marković, B., Dobričić, V., Milosavljević, P., Nepovimova, E.,& Kuča, K.. (2023). in Chemico-biological interactions
Elsevier B.V.., 383, 110658.
https://doi.org/10.1016/j.cbi.2023.110658

Jaćević V, Dumanović J, Grujić-Milanović J, Milovanović Z, Amidžić L, Vojinović N, Nežić L, Marković B, Dobričić V, Milosavljević P, Nepovimova E, Kuča K. in Chemico-biological interactions. 2023;383:110658.
doi:10.1016/j.cbi.2023.110658 .

Jaćević, Vesna, Dumanović, Jelena, Grujić-Milanović, Jelica, Milovanović, Zoran, Amidžić, Ljiljana, Vojinović, Nataša, Nežić, Lana, Marković, Bojan, Dobričić, Vladimir, Milosavljević, Petar, Nepovimova, Eugenie, Kuča, Kamil, in Chemico-biological interactions, 383 (2023):110658,
https://doi.org/10.1016/j.cbi.2023.110658 . .

TY  - JOUR
AU  - Porat, Daniel
AU  - Dukhno, Oleg
AU  - Partook-Maccabi, Mazal
AU  - Vainer, Ella
AU  - Cvijić, Sandra
AU  - Dahan, Arik
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5038
AB  - Anatomical/physiological gastrointestinal changes after bariatric surgery may influence the fate of orally administered drugs. Since non-selective NSAIDs are not well-tolerated post-surgery, selective cyclooxygenase-2 (COX-2) inhibitors may be important for these patients. In this work we investigated celecoxib, etoricoxib and etodolac, for impaired post-bariatric solubility/dissolution and absorption. Solubility was studied in-vitro, and ex-vivo in aspirated gastric contents from patients pre- vs. post-surgery. Dissolution was studied in conditions simulating pre- vs. post-surgery stomach. Finally, the experimental solubility data were used in physiologically-based biopharmaceutics model (PBBM) (GastroPlus®) to simulate pre- vs. post-surgery celecoxib/etoricoxib/etodolac pharmacokinetic (PK) profiles. For etoricoxib and etodolac (but not celecoxib), pH-dependent solubility was demonstrated: etoricoxib solubility decreased ∼1000-fold, and etodolac solubility increased 120-fold, as pH increased from 1 to 7, which was also confirmed ex-vivo. Hampered etoricoxib dissolution and improved etodolac dissolution post-surgery was revealed. Tablet crushing, clinically recommended after surgery, failed to improve post-bariatric dissolution. PBBM simulations revealed significantly impaired etoricoxib absorption post-surgery across all conditions; for instance, 79% lower Cmax and 53% decreased AUC was simulated post-gastric bypass procedure, after single 120 mg dose. Celecoxib and etodolac maintained unaffected absorption after bariatric surgery. This mechanistically-based analysis suggests to prefer the acidic drug etodolac or the neutral celecoxib as selective COX-2 inhibitors, over the basic drug etoricoxib, after bariatric surgery.
PB  - Elsevier B.V.
T2  - International Journal of Pharmaceutics
T1  - Selective COX-2 inhibitors after bariatric surgery: Celecoxib, etoricoxib and etodolac post-bariatric solubility/dissolution and pharmacokinetics
VL  - 645
DO  - 10.1016/j.ijpharm.2023.123347
ER  - 

@article{
author = "Porat, Daniel and Dukhno, Oleg and Partook-Maccabi, Mazal and Vainer, Ella and Cvijić, Sandra and Dahan, Arik",
year = "2023",
abstract = "Anatomical/physiological gastrointestinal changes after bariatric surgery may influence the fate of orally administered drugs. Since non-selective NSAIDs are not well-tolerated post-surgery, selective cyclooxygenase-2 (COX-2) inhibitors may be important for these patients. In this work we investigated celecoxib, etoricoxib and etodolac, for impaired post-bariatric solubility/dissolution and absorption. Solubility was studied in-vitro, and ex-vivo in aspirated gastric contents from patients pre- vs. post-surgery. Dissolution was studied in conditions simulating pre- vs. post-surgery stomach. Finally, the experimental solubility data were used in physiologically-based biopharmaceutics model (PBBM) (GastroPlus®) to simulate pre- vs. post-surgery celecoxib/etoricoxib/etodolac pharmacokinetic (PK) profiles. For etoricoxib and etodolac (but not celecoxib), pH-dependent solubility was demonstrated: etoricoxib solubility decreased ∼1000-fold, and etodolac solubility increased 120-fold, as pH increased from 1 to 7, which was also confirmed ex-vivo. Hampered etoricoxib dissolution and improved etodolac dissolution post-surgery was revealed. Tablet crushing, clinically recommended after surgery, failed to improve post-bariatric dissolution. PBBM simulations revealed significantly impaired etoricoxib absorption post-surgery across all conditions; for instance, 79% lower Cmax and 53% decreased AUC was simulated post-gastric bypass procedure, after single 120 mg dose. Celecoxib and etodolac maintained unaffected absorption after bariatric surgery. This mechanistically-based analysis suggests to prefer the acidic drug etodolac or the neutral celecoxib as selective COX-2 inhibitors, over the basic drug etoricoxib, after bariatric surgery.",
publisher = "Elsevier B.V.",
journal = "International Journal of Pharmaceutics",
title = "Selective COX-2 inhibitors after bariatric surgery: Celecoxib, etoricoxib and etodolac post-bariatric solubility/dissolution and pharmacokinetics",
volume = "645",
doi = "10.1016/j.ijpharm.2023.123347"
}

Porat, D., Dukhno, O., Partook-Maccabi, M., Vainer, E., Cvijić, S.,& Dahan, A.. (2023). Selective COX-2 inhibitors after bariatric surgery: Celecoxib, etoricoxib and etodolac post-bariatric solubility/dissolution and pharmacokinetics. in International Journal of Pharmaceutics
Elsevier B.V.., 645.
https://doi.org/10.1016/j.ijpharm.2023.123347

Porat D, Dukhno O, Partook-Maccabi M, Vainer E, Cvijić S, Dahan A. Selective COX-2 inhibitors after bariatric surgery: Celecoxib, etoricoxib and etodolac post-bariatric solubility/dissolution and pharmacokinetics. in International Journal of Pharmaceutics. 2023;645.
doi:10.1016/j.ijpharm.2023.123347 .

Porat, Daniel, Dukhno, Oleg, Partook-Maccabi, Mazal, Vainer, Ella, Cvijić, Sandra, Dahan, Arik, "Selective COX-2 inhibitors after bariatric surgery: Celecoxib, etoricoxib and etodolac post-bariatric solubility/dissolution and pharmacokinetics" in International Journal of Pharmaceutics, 645 (2023),
https://doi.org/10.1016/j.ijpharm.2023.123347 . .

TY  - JOUR
AU  - Lević, Marija
AU  - Bogavac-Stanojević, Nataša
AU  - Ubavić, Stana
AU  - Krajnović, Dušanka
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5037
AB  - Background: Pharmacotherapy literacy (PTHL) is a specific ability to safely access, appraise and understand the available information concerning medication and to calculate and act accordingly. The concept of PTHL is mostly unknown for the majority of diabetes mellitus type 2 (DMT2) patients in Serbia. With diabetes being one of the major public health problems in Serbia with a prevalence of 9.1%, this two-study research aims at constructing performance-based instrument and estimating the prevalence of PTHL levels and identification of predictors of low PTHL scores in patients with DMT2. Methods: Multistage study was performed to adapt the existing performance–based instrument (PTHL-SR) into specific questionnaire for DMT2 population (PTHL-DM instrument). PTHL levels were assessed through cross-sectional study categorising patients into groups of low, medium, and high PTHL levels. We considered 19 predictors for low PTHL scores, from sociodemographic characteristics, health behaviours and health characteristics, access to health-related information and empowerment-related indicators. Univariate and multivariate logistic regression analyses were used to determine independent predictors of low PTHL. Results: The final 15-item PTHL-DM instrument proved to have satisfactory reliability (KR20 = 0.475) and internal reliability [ICC for the whole instrument was 0.97 with 95% confidence intervals (0.95–0.99)]. Positive correlation (rho = 0.69) between PTHL-DM score (15 questions) and the total PTHL-SR score (14 questions) was also observed. It was demonstrated that the majority of 350 patients had low PTHL (62%), and only 5% high PTHL level. Mean score on PTHL-DM was 7.8 ± 2.3. Probability of low PTHL increased among smokers, patients with low interest in health and those who estimated their health as bad. Patients who used pharmacists as sourse of information were less likely to be pharmacotherapy illiterate. Combined therapy with insulin and Oral Hypoglycemic Agents was associated with higher PTHL. Conclusions: Our data indicate that specific PTHL-DM tool is objective, valid, and reliable. It was found that low level of PTHL prevailed among DMT2 patients. Medication literacy is influenced by age, residence, education, and family status. Patients with better health literacy also reported better health behaviours. Different patient empowerment programs and approaches aimed at raising PTHL would be essential to improve self-management and control of this widespread chronic disease in Serbia.
PB  - BioMed Central Ltd
T2  - BMC Public Health
T1  - Pharmacotherapy literacy level and predictors of low literacy among diabetes mellitus type 2 patients in Serbia
VL  - 23
IS  - 1
DO  - 10.1186/s12889-023-16639-y
ER  - 

@article{
author = "Lević, Marija and Bogavac-Stanojević, Nataša and Ubavić, Stana and Krajnović, Dušanka",
year = "2023",
abstract = "Background: Pharmacotherapy literacy (PTHL) is a specific ability to safely access, appraise and understand the available information concerning medication and to calculate and act accordingly. The concept of PTHL is mostly unknown for the majority of diabetes mellitus type 2 (DMT2) patients in Serbia. With diabetes being one of the major public health problems in Serbia with a prevalence of 9.1%, this two-study research aims at constructing performance-based instrument and estimating the prevalence of PTHL levels and identification of predictors of low PTHL scores in patients with DMT2. Methods: Multistage study was performed to adapt the existing performance–based instrument (PTHL-SR) into specific questionnaire for DMT2 population (PTHL-DM instrument). PTHL levels were assessed through cross-sectional study categorising patients into groups of low, medium, and high PTHL levels. We considered 19 predictors for low PTHL scores, from sociodemographic characteristics, health behaviours and health characteristics, access to health-related information and empowerment-related indicators. Univariate and multivariate logistic regression analyses were used to determine independent predictors of low PTHL. Results: The final 15-item PTHL-DM instrument proved to have satisfactory reliability (KR20 = 0.475) and internal reliability [ICC for the whole instrument was 0.97 with 95% confidence intervals (0.95–0.99)]. Positive correlation (rho = 0.69) between PTHL-DM score (15 questions) and the total PTHL-SR score (14 questions) was also observed. It was demonstrated that the majority of 350 patients had low PTHL (62%), and only 5% high PTHL level. Mean score on PTHL-DM was 7.8 ± 2.3. Probability of low PTHL increased among smokers, patients with low interest in health and those who estimated their health as bad. Patients who used pharmacists as sourse of information were less likely to be pharmacotherapy illiterate. Combined therapy with insulin and Oral Hypoglycemic Agents was associated with higher PTHL. Conclusions: Our data indicate that specific PTHL-DM tool is objective, valid, and reliable. It was found that low level of PTHL prevailed among DMT2 patients. Medication literacy is influenced by age, residence, education, and family status. Patients with better health literacy also reported better health behaviours. Different patient empowerment programs and approaches aimed at raising PTHL would be essential to improve self-management and control of this widespread chronic disease in Serbia.",
publisher = "BioMed Central Ltd",
journal = "BMC Public Health",
title = "Pharmacotherapy literacy level and predictors of low literacy among diabetes mellitus type 2 patients in Serbia",
volume = "23",
number = "1",
doi = "10.1186/s12889-023-16639-y"
}

Lević, M., Bogavac-Stanojević, N., Ubavić, S.,& Krajnović, D.. (2023). Pharmacotherapy literacy level and predictors of low literacy among diabetes mellitus type 2 patients in Serbia. in BMC Public Health
BioMed Central Ltd., 23(1).
https://doi.org/10.1186/s12889-023-16639-y

Lević M, Bogavac-Stanojević N, Ubavić S, Krajnović D. Pharmacotherapy literacy level and predictors of low literacy among diabetes mellitus type 2 patients in Serbia. in BMC Public Health. 2023;23(1).
doi:10.1186/s12889-023-16639-y .

Lević, Marija, Bogavac-Stanojević, Nataša, Ubavić, Stana, Krajnović, Dušanka, "Pharmacotherapy literacy level and predictors of low literacy among diabetes mellitus type 2 patients in Serbia" in BMC Public Health, 23, no. 1 (2023),
https://doi.org/10.1186/s12889-023-16639-y . .

TY  - JOUR
AU  - Ušjak, Ljuboš
AU  - Drobac, Milica
AU  - Ivanov, Marija
AU  - Soković, Marina
AU  - Milenković, Marina
AU  - Niketić, Marjan
AU  - Petrović, Silvana
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4469
AB  - Composition and antimicrobial activity of root, leaf, stem, flower and fruit essential oils from
cultivated Pastinaca sativa subsp. sativa, and its two wild-growing relatives P. sativa subsp. urens
and P. hirsuta (Apiaceae) were investigated. Twenty-nine hydrodistilled essential oils of plants from
different localities and/or years were analysed by GC-FID and GC/MS. Dominant in root oils was
myristicin (P. sativa) or apiole (P. hirsuta), in leaf and stem oils myristicin (cultivated plants) or γ-palmitolactone (wild-growing plants) and in flower and fruit oils aliphatic esters. Multivariate
statistics (PCA, nMDS, UPGMA clustering) generally revealed separation of oils of investigated
Pastinaca taxa and demonstrated their chemosystematic significance. One oil per each organ of all three plants (fifteen in total) was tested using microdilution method for activity against Candida
tropicalis, C. parapsilosis, C. krusei, C. glabrata, C. albicans, Staphylococcus aureus, Bacillus cereus, Listeria monocytogenes, Escherichia coli, Salmonella Typhimurium and Enterobacter cloacae; MIC = 0.25–8 mg/mL, MBC(MFC) = 0.5–16 mg/mL.
PB  - Taylor and Francis Group
T2  - Journal of Essential Oil Research
T1  - Composition and antimicrobial activity of Pastinaca sativa subsp. sativa, P. sativa subsp. urens and P. hirsuta essential oils
VL  - 35
IS  - 2
SP  - 154
EP  - 167
DO  - 10.1080/10412905.2022.2147675
ER  - 

@article{
author = "Ušjak, Ljuboš and Drobac, Milica and Ivanov, Marija and Soković, Marina and Milenković, Marina and Niketić, Marjan and Petrović, Silvana",
year = "2023",
abstract = "Composition and antimicrobial activity of root, leaf, stem, flower and fruit essential oils from
cultivated Pastinaca sativa subsp. sativa, and its two wild-growing relatives P. sativa subsp. urens
and P. hirsuta (Apiaceae) were investigated. Twenty-nine hydrodistilled essential oils of plants from
different localities and/or years were analysed by GC-FID and GC/MS. Dominant in root oils was
myristicin (P. sativa) or apiole (P. hirsuta), in leaf and stem oils myristicin (cultivated plants) or γ-palmitolactone (wild-growing plants) and in flower and fruit oils aliphatic esters. Multivariate
statistics (PCA, nMDS, UPGMA clustering) generally revealed separation of oils of investigated
Pastinaca taxa and demonstrated their chemosystematic significance. One oil per each organ of all three plants (fifteen in total) was tested using microdilution method for activity against Candida
tropicalis, C. parapsilosis, C. krusei, C. glabrata, C. albicans, Staphylococcus aureus, Bacillus cereus, Listeria monocytogenes, Escherichia coli, Salmonella Typhimurium and Enterobacter cloacae; MIC = 0.25–8 mg/mL, MBC(MFC) = 0.5–16 mg/mL.",
publisher = "Taylor and Francis Group",
journal = "Journal of Essential Oil Research",
title = "Composition and antimicrobial activity of Pastinaca sativa subsp. sativa, P. sativa subsp. urens and P. hirsuta essential oils",
volume = "35",
number = "2",
pages = "154-167",
doi = "10.1080/10412905.2022.2147675"
}

Ušjak, L., Drobac, M., Ivanov, M., Soković, M., Milenković, M., Niketić, M.,& Petrović, S.. (2023). Composition and antimicrobial activity of Pastinaca sativa subsp. sativa, P. sativa subsp. urens and P. hirsuta essential oils. in Journal of Essential Oil Research
Taylor and Francis Group., 35(2), 154-167.
https://doi.org/10.1080/10412905.2022.2147675

Ušjak L, Drobac M, Ivanov M, Soković M, Milenković M, Niketić M, Petrović S. Composition and antimicrobial activity of Pastinaca sativa subsp. sativa, P. sativa subsp. urens and P. hirsuta essential oils. in Journal of Essential Oil Research. 2023;35(2):154-167.
doi:10.1080/10412905.2022.2147675 .

Ušjak, Ljuboš, Drobac, Milica, Ivanov, Marija, Soković, Marina, Milenković, Marina, Niketić, Marjan, Petrović, Silvana, "Composition and antimicrobial activity of Pastinaca sativa subsp. sativa, P. sativa subsp. urens and P. hirsuta essential oils" in Journal of Essential Oil Research, 35, no. 2 (2023):154-167,
https://doi.org/10.1080/10412905.2022.2147675 . .

TY  - CONF
AU  - Plankoš, Aleksandar
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4792
AB  - Увод: Бележи се пораст јављања менталних болести, па чак 1 од 8 особа живи са неким менталним поремећајем. Лекови који проналазе јединствену улогу у лечењу истих јесу антипсихотици, међу којима клозапин чини основу терапије на третман резистентне схизофреније. Клозапин својим плејотропним дејством на рецепторе у централном нервном систему остварује своје терапијске ефекте. Како је метаболизам клозапина посредован CYP450 ензимима, то представља основу за испољавање фармакокинетичких интеракција. 
Циљ рада: Идентификација, одређивање заступљености и класе интеракција код пацијената са психијатријским обољењем помоћу два извора података.
Материјал и методе: У истраживање је било укључено 33 пацијента са дијагностикованим психијатријским обољењем који су као основну терапију имали клозапин. Идентификација и карактеризација потенцијалних интеракција вршена је помоћу Drug Interactions Checker и Epocrates базе података.
Резултати: Идентификовано је укупно 308 интеракција, а утврђена је значајна корелација између података добијених помоћу ове две базе. Заступљеност фармакокинетичких интеракција била 17,1% по Drug Interactions Checker бази и 12% према Epocrates бази. Идентификоване су 52 интеракције највишег степена озбиљности. Дневна доза клозапина је за 54,5 mg била већа код пацијената са идентификованим интеракцијама према Drug Interactions Checker бази.
Закључак: Висока преваленца интеракција је идентификована применом обе базе података за интеракције. Такође, како Drug Interactions Checker база даје опширнији увид у идентификоване интеракције, закључује се да би њена примена у пракси била од значаја приликом планирања терапије како би се правовремено предвиделе и избегле потенцијалне нежељене интеракције.
AB  - Introduction: There is an increase in the occurrence of mental illnesses, and as many as 1 in 8 people live with some mental disorder. Drugs that have a unique role in the treatment of these desorders are antipsychotics, among which clozapine forms the basis of therapy for the treatment resistant schizophrenia. Clozapine achieves its therapeutic effects through its pleiotropic effect on receptors in the central nervous system. Since the metabolism of clozapine is mediated by CYP450 enzymes, it is the basis for the manifestation of pharmacokinetic interactions.
The Aim: Identification, determination of prevalence and class of interactions in psychiatric patients using two data sources.
Material and Methods: The study included 33 patients with a diagnosed psychiatric illness who had clozapine as their primary therapy. Identification and characterization of potential interactions was performed using the Drug Interactions Checker and the Epocrates database.
Results: A total of 308 interactions were identified, and a significant correlation was established between the data obtained using these two databases. The prevalence of pharmacokinetic interactions was 17.1% according to the Drug Interactions Checker database and 12% according to the Epocrates database. 52 interactions of the highest severity were identified. The daily dose of clozapine was 54.5 mg higher in patients with identified interactions according to Drug Interactions Checker.
Conclusion: A high prevalence of interactions was identified using both interaction databases. Also, as the Drug Interactions Checker database provides more extensive insight into identified interactions, it is concluded that it’s application in practice would be important when planning therapy in order to timely predict and avoid potential unwanted interactions.
T1  - Одређивање заступљености и  карактеристика интеракција лекова код пацијената са психијатријским обољењем
T1  - Determination of prevalence and characteristics of drug interactions in patients with psychiatric disease
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4792
ER  - 

@conference{
author = "Plankoš, Aleksandar",
year = "2023",
abstract = "Увод: Бележи се пораст јављања менталних болести, па чак 1 од 8 особа живи са неким менталним поремећајем. Лекови који проналазе јединствену улогу у лечењу истих јесу антипсихотици, међу којима клозапин чини основу терапије на третман резистентне схизофреније. Клозапин својим плејотропним дејством на рецепторе у централном нервном систему остварује своје терапијске ефекте. Како је метаболизам клозапина посредован CYP450 ензимима, то представља основу за испољавање фармакокинетичких интеракција. 
Циљ рада: Идентификација, одређивање заступљености и класе интеракција код пацијената са психијатријским обољењем помоћу два извора података.
Материјал и методе: У истраживање је било укључено 33 пацијента са дијагностикованим психијатријским обољењем који су као основну терапију имали клозапин. Идентификација и карактеризација потенцијалних интеракција вршена је помоћу Drug Interactions Checker и Epocrates базе података.
Резултати: Идентификовано је укупно 308 интеракција, а утврђена је значајна корелација између података добијених помоћу ове две базе. Заступљеност фармакокинетичких интеракција била 17,1% по Drug Interactions Checker бази и 12% према Epocrates бази. Идентификоване су 52 интеракције највишег степена озбиљности. Дневна доза клозапина је за 54,5 mg била већа код пацијената са идентификованим интеракцијама према Drug Interactions Checker бази.
Закључак: Висока преваленца интеракција је идентификована применом обе базе података за интеракције. Такође, како Drug Interactions Checker база даје опширнији увид у идентификоване интеракције, закључује се да би њена примена у пракси била од значаја приликом планирања терапије како би се правовремено предвиделе и избегле потенцијалне нежељене интеракције., Introduction: There is an increase in the occurrence of mental illnesses, and as many as 1 in 8 people live with some mental disorder. Drugs that have a unique role in the treatment of these desorders are antipsychotics, among which clozapine forms the basis of therapy for the treatment resistant schizophrenia. Clozapine achieves its therapeutic effects through its pleiotropic effect on receptors in the central nervous system. Since the metabolism of clozapine is mediated by CYP450 enzymes, it is the basis for the manifestation of pharmacokinetic interactions.
The Aim: Identification, determination of prevalence and class of interactions in psychiatric patients using two data sources.
Material and Methods: The study included 33 patients with a diagnosed psychiatric illness who had clozapine as their primary therapy. Identification and characterization of potential interactions was performed using the Drug Interactions Checker and the Epocrates database.
Results: A total of 308 interactions were identified, and a significant correlation was established between the data obtained using these two databases. The prevalence of pharmacokinetic interactions was 17.1% according to the Drug Interactions Checker database and 12% according to the Epocrates database. 52 interactions of the highest severity were identified. The daily dose of clozapine was 54.5 mg higher in patients with identified interactions according to Drug Interactions Checker.
Conclusion: A high prevalence of interactions was identified using both interaction databases. Also, as the Drug Interactions Checker database provides more extensive insight into identified interactions, it is concluded that it’s application in practice would be important when planning therapy in order to timely predict and avoid potential unwanted interactions.",
title = "Одређивање заступљености и  карактеристика интеракција лекова код пацијената са психијатријским обољењем, Determination of prevalence and characteristics of drug interactions in patients with psychiatric disease",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4792"
}

Plankoš, A.. (2023). Одређивање заступљености и  карактеристика интеракција лекова код пацијената са психијатријским обољењем. .
https://hdl.handle.net/21.15107/rcub_farfar_4792

Plankoš A. Одређивање заступљености и  карактеристика интеракција лекова код пацијената са психијатријским обољењем. 2023;.
https://hdl.handle.net/21.15107/rcub_farfar_4792 .

Plankoš, Aleksandar, "Одређивање заступљености и  карактеристика интеракција лекова код пацијената са психијатријским обољењем" (2023),
https://hdl.handle.net/21.15107/rcub_farfar_4792 .

TY  - JOUR
AU  - Jovanović, Marija
AU  - Kovačević, Milena
AU  - Catić-Đorđević, Aleksandra
AU  - Ćulafić, Milica
AU  - Stefanović, Nikola
AU  - Mitić, Branka
AU  - Vučićević, Katarina
AU  - Vezmar-Kovačević, Sandra
AU  - Veličković-Radovanović, Radmila
AU  - Miljković, Branislava
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4921
AB  - The study aimed to estimate and compare the prevalence and type of potentially inappropriate medications (PIMs) and potential prescribing omissions (PPOs) between the STOPP/START original (v1) and updated version (v2) among older patients in various settings, as well as associated factors. The study included 440 patients attending a community pharmacy, 200 outpatients and 140 nursing home users. An increase in the prevalence of STOPP v2 (57.9%) compared to v1 (56.2%) was not statistically significant in the total sample and within each setting (p>0.05). A decrease in the prevalence of START v1 (55.8%) to v2 (41.2%) was statistically significant (p<0.001) in the total sample and within each setting (p<0.05). Drug indication (32.9%) and fall-risk medications (32.2%) were most commonly identified for STOPP v2, while cardiovascular system criteria (30.5%) were the most frequently detected for START v2. The number of medications was the strongest predictor for both STOPP v1 and v2, with odds ratio values of 1.35 and 1.34, respectively. Patients’ characteristics associated with the occurrence of STOPP and START criteria were identified. According to both STOPP/START versions, the results indicate a substantial rate of potentially inappropriate prescribing among elderly patients. The prevalence of PIMs was slightly higher with the updated version, while the prevalence of PPOs was significantly lower.
T2  - Brazilian Journal of Pharmaceutical Sciences
T1  - Potentially inappropriate prescribing among older patients and associated factors: comparison of two versions of STOPP/START criteria
VL  - 59
DO  - 10.1590/s2175-97902023e22549
ER  - 

@article{
author = "Jovanović, Marija and Kovačević, Milena and Catić-Đorđević, Aleksandra and Ćulafić, Milica and Stefanović, Nikola and Mitić, Branka and Vučićević, Katarina and Vezmar-Kovačević, Sandra and Veličković-Radovanović, Radmila and Miljković, Branislava",
year = "2023",
abstract = "The study aimed to estimate and compare the prevalence and type of potentially inappropriate medications (PIMs) and potential prescribing omissions (PPOs) between the STOPP/START original (v1) and updated version (v2) among older patients in various settings, as well as associated factors. The study included 440 patients attending a community pharmacy, 200 outpatients and 140 nursing home users. An increase in the prevalence of STOPP v2 (57.9%) compared to v1 (56.2%) was not statistically significant in the total sample and within each setting (p>0.05). A decrease in the prevalence of START v1 (55.8%) to v2 (41.2%) was statistically significant (p<0.001) in the total sample and within each setting (p<0.05). Drug indication (32.9%) and fall-risk medications (32.2%) were most commonly identified for STOPP v2, while cardiovascular system criteria (30.5%) were the most frequently detected for START v2. The number of medications was the strongest predictor for both STOPP v1 and v2, with odds ratio values of 1.35 and 1.34, respectively. Patients’ characteristics associated with the occurrence of STOPP and START criteria were identified. According to both STOPP/START versions, the results indicate a substantial rate of potentially inappropriate prescribing among elderly patients. The prevalence of PIMs was slightly higher with the updated version, while the prevalence of PPOs was significantly lower.",
journal = "Brazilian Journal of Pharmaceutical Sciences",
title = "Potentially inappropriate prescribing among older patients and associated factors: comparison of two versions of STOPP/START criteria",
volume = "59",
doi = "10.1590/s2175-97902023e22549"
}

Jovanović, M., Kovačević, M., Catić-Đorđević, A., Ćulafić, M., Stefanović, N., Mitić, B., Vučićević, K., Vezmar-Kovačević, S., Veličković-Radovanović, R.,& Miljković, B.. (2023). Potentially inappropriate prescribing among older patients and associated factors: comparison of two versions of STOPP/START criteria. in Brazilian Journal of Pharmaceutical Sciences, 59.
https://doi.org/10.1590/s2175-97902023e22549

Jovanović M, Kovačević M, Catić-Đorđević A, Ćulafić M, Stefanović N, Mitić B, Vučićević K, Vezmar-Kovačević S, Veličković-Radovanović R, Miljković B. Potentially inappropriate prescribing among older patients and associated factors: comparison of two versions of STOPP/START criteria. in Brazilian Journal of Pharmaceutical Sciences. 2023;59.
doi:10.1590/s2175-97902023e22549 .

Jovanović, Marija, Kovačević, Milena, Catić-Đorđević, Aleksandra, Ćulafić, Milica, Stefanović, Nikola, Mitić, Branka, Vučićević, Katarina, Vezmar-Kovačević, Sandra, Veličković-Radovanović, Radmila, Miljković, Branislava, "Potentially inappropriate prescribing among older patients and associated factors: comparison of two versions of STOPP/START criteria" in Brazilian Journal of Pharmaceutical Sciences, 59 (2023),
https://doi.org/10.1590/s2175-97902023e22549 . .

TY  - CONF
AU  - Roganović, Maša
AU  - Stanojković, Tatjana
AU  - Nikitović, Marina
AU  - Petrović, Nina
AU  - Đurić, Ana
AU  - Matić, Ivana
AU  - Jovanović, Marija
AU  - Vučićević, Katarina
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4899
AB  - Introduction: The second most common form of cancer in the male population is prostate cancer. Therapeutic options include radical prostatectomy, different forms of radiotherapy, hormone treatment and chemotherapy. Radical prostatectomy and radiotherapy are the most frequently used strategies and enable a long survival for patients diagnosed on time. Because of the prostate anatomy, patients that are on radiotherapy experience a great range of side effects, and even after the therapy is finished, side effects such as urogenital and gastrointestinal toxicity can persist for years. Although survival is long regardless of the therapeutic option used, choosing the appropriate therapeutic options for the patients in terms of efficacy and safety is very important [1]. 

Objectives: We aimed in developing a model for repeated count data, i.e. fatigue, which is the most common adverse event that patients experience during radiotherapy. In addition, the objective of the study is to assess the effect of various covariates on the probability of the event happening using different modelling approaches [2]. 

Methods: Data collected from prostate cancer patients included: age, concentrations of glutamine and glutamate before radiotherapy and after 5, 15, 25 and 30 fractions of radiotherapy, as well as a month after the last fraction of radiotherapy (first follow – up visit), genetic testing results regarding variants in glutamine metabolic pathway, signs and symptoms of acute or chronic urogenital and gastrointestinal toxicity, fatigue, details of their treatment (e.g. radical prostatectomy, hormone therapy), their smoking and alcohol intake status, presence of hypertension or diabetes mellitus type II, and other laboratory findings of significance. Analysis was performed using nonlinear mixed effects modelling approach using NONMEM® software (version 7.4). We tested two approaches: modelling using the first-order estimation method and the Laplace method of estimation in order to create a Poisson model for count data. NONMEM outputs were handled in R software (graphical diagnostics). Model evaluation has been performed using numerical and visual approaches. Covariate model building was performed using a stepwise covariate procedure (SCM). Covariates that were tested are age, glutamine/glutamate concentrations (continuous, time-varying covariates). The influence of categorical covariates was also examined (smoking and alcohol intake, presence of aforementioned comorbidities). 

Results: In total, we analysed 143 data records from 28 male patients aged 53-82 years (mean±sd: 72.67±6.64), mainly older people (>65 years old) that were included in the analysis. The probability of fatigue occurrence was 78.3%, which was rather high but expected. The objective function value of the developed base model using the Laplace method of estimation was 546.346. The average number of fatigue events occurring in the period from the start of the radiotherapy until the first follow-up visit was estimated to be 2.48 with a 95% confidence interval of 1.655 - 3.305 and RSE of 17%. Interindividual variability in the number of fatigue events per patient was estimated at 48.3%, with a shrinkage of 11.1%. The inclusion of the covariates in the base model did not improve the model fit, so they were not kept in the model. 

Conclusion: Our results confirm that fatigue is one of the most common side effects of radiotherapy. Although our model did not show that examined covariates have an effect on the average number of fatigue events,  further analysis will aim at testing different modelling approaches when it comes to modelling side effects of radiotherapy in order to minimize them in cancer patients. 



[1] Retrieved from https://www.nhs.uk/conditions/prostate-cancer/treatment/ . Last access: 19.3.2023.
[2] Plan E.L. Modeling and simulation of count data.  CPT Pharmacometrics Syst. Pharmacol. 2014: 3 (8): p. e129.
C3  - Page. Abstracts of the Annual Meeting of the Population Approach Group in Europe
T1  - Modelling fatigue events in prostate cancer patients on radiotherapy
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4899
ER  - 

@conference{
author = "Roganović, Maša and Stanojković, Tatjana and Nikitović, Marina and Petrović, Nina and Đurić, Ana and Matić, Ivana and Jovanović, Marija and Vučićević, Katarina",
year = "2023",
abstract = "Introduction: The second most common form of cancer in the male population is prostate cancer. Therapeutic options include radical prostatectomy, different forms of radiotherapy, hormone treatment and chemotherapy. Radical prostatectomy and radiotherapy are the most frequently used strategies and enable a long survival for patients diagnosed on time. Because of the prostate anatomy, patients that are on radiotherapy experience a great range of side effects, and even after the therapy is finished, side effects such as urogenital and gastrointestinal toxicity can persist for years. Although survival is long regardless of the therapeutic option used, choosing the appropriate therapeutic options for the patients in terms of efficacy and safety is very important [1]. 

Objectives: We aimed in developing a model for repeated count data, i.e. fatigue, which is the most common adverse event that patients experience during radiotherapy. In addition, the objective of the study is to assess the effect of various covariates on the probability of the event happening using different modelling approaches [2]. 

Methods: Data collected from prostate cancer patients included: age, concentrations of glutamine and glutamate before radiotherapy and after 5, 15, 25 and 30 fractions of radiotherapy, as well as a month after the last fraction of radiotherapy (first follow – up visit), genetic testing results regarding variants in glutamine metabolic pathway, signs and symptoms of acute or chronic urogenital and gastrointestinal toxicity, fatigue, details of their treatment (e.g. radical prostatectomy, hormone therapy), their smoking and alcohol intake status, presence of hypertension or diabetes mellitus type II, and other laboratory findings of significance. Analysis was performed using nonlinear mixed effects modelling approach using NONMEM® software (version 7.4). We tested two approaches: modelling using the first-order estimation method and the Laplace method of estimation in order to create a Poisson model for count data. NONMEM outputs were handled in R software (graphical diagnostics). Model evaluation has been performed using numerical and visual approaches. Covariate model building was performed using a stepwise covariate procedure (SCM). Covariates that were tested are age, glutamine/glutamate concentrations (continuous, time-varying covariates). The influence of categorical covariates was also examined (smoking and alcohol intake, presence of aforementioned comorbidities). 

Results: In total, we analysed 143 data records from 28 male patients aged 53-82 years (mean±sd: 72.67±6.64), mainly older people (>65 years old) that were included in the analysis. The probability of fatigue occurrence was 78.3%, which was rather high but expected. The objective function value of the developed base model using the Laplace method of estimation was 546.346. The average number of fatigue events occurring in the period from the start of the radiotherapy until the first follow-up visit was estimated to be 2.48 with a 95% confidence interval of 1.655 - 3.305 and RSE of 17%. Interindividual variability in the number of fatigue events per patient was estimated at 48.3%, with a shrinkage of 11.1%. The inclusion of the covariates in the base model did not improve the model fit, so they were not kept in the model. 

Conclusion: Our results confirm that fatigue is one of the most common side effects of radiotherapy. Although our model did not show that examined covariates have an effect on the average number of fatigue events,  further analysis will aim at testing different modelling approaches when it comes to modelling side effects of radiotherapy in order to minimize them in cancer patients. 



[1] Retrieved from https://www.nhs.uk/conditions/prostate-cancer/treatment/ . Last access: 19.3.2023.
[2] Plan E.L. Modeling and simulation of count data.  CPT Pharmacometrics Syst. Pharmacol. 2014: 3 (8): p. e129.",
journal = "Page. Abstracts of the Annual Meeting of the Population Approach Group in Europe",
title = "Modelling fatigue events in prostate cancer patients on radiotherapy",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4899"
}

Roganović, M., Stanojković, T., Nikitović, M., Petrović, N., Đurić, A., Matić, I., Jovanović, M.,& Vučićević, K.. (2023). Modelling fatigue events in prostate cancer patients on radiotherapy. in Page. Abstracts of the Annual Meeting of the Population Approach Group in Europe.
https://hdl.handle.net/21.15107/rcub_farfar_4899

Roganović M, Stanojković T, Nikitović M, Petrović N, Đurić A, Matić I, Jovanović M, Vučićević K. Modelling fatigue events in prostate cancer patients on radiotherapy. in Page. Abstracts of the Annual Meeting of the Population Approach Group in Europe. 2023;.
https://hdl.handle.net/21.15107/rcub_farfar_4899 .

Roganović, Maša, Stanojković, Tatjana, Nikitović, Marina, Petrović, Nina, Đurić, Ana, Matić, Ivana, Jovanović, Marija, Vučićević, Katarina, "Modelling fatigue events in prostate cancer patients on radiotherapy" in Page. Abstracts of the Annual Meeting of the Population Approach Group in Europe (2023),
https://hdl.handle.net/21.15107/rcub_farfar_4899 .

TY  - CONF
AU  - Homšek, Ana
AU  - Marković, Srđan
AU  - Kralj, Đorđe
AU  - Odanović, Olga
AU  - Svorcan, Petar
AU  - Jovanović, Marija
AU  - Vučićević, Katarina
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4898
AB  - Introduction: Infliximab (IFX), anti-tumour necrosis factor alpha antibody, is widely used in the treatment of inflammatory bowel diseases. Patients with Crohn’s disease may develop a more complicated form which involves fistulae formation, most commonly perianal. IFX has been effective in inducing fistula closure and maintaining disease remission [1]. In order to achieve fistulae healing adequate trough concentrations should be achieved [2].

Objectives:

Obtaining PK parameters for patients with the fistulising disease form
Comparing the results to the ones available in the literature for Crohn’s disease
Methods: In a retrospective review of medical documentation (from 2013 to present), 42 patients (64% male, 18-65 years old) with fistulising Crohn’s disease were selected for analysis. IFX concentrations were measured just before dose administration (trough concentration) or earlier if the patient was experiencing severe disease symptoms. For the majority of patients concentration was measured at week 14, right before the first maintenance dose. Additional concentrations were obtained at scheduled check-ups or after patients experienced signs of relapse. After appropriate premedication, each patient received the calculated fixed dose based on their body weight (dose range was 200-800 mg) via two-hour infusion. The drug was administered according to the standard (week 0, 2 and 6) and accelerated induction (every two weeks) protocols, followed by maintenance therapy (every 4, 6 or 8 weeks). Population modelling approach was applied to characterize pharmacokinetic profile of IFX using NONMEM 7.3 software (ICON Development Solutions Inc., Dublin, Ireland) [3]. The obtained data were processed using Microsoft Excel and R software.

Results: After exclusion of concentrations over the upper limit of quantification (12 μg/mL), a total of 161 concentrations were analysed. IFX concentrations below lower limit of quantification were accounted for using the M5 method [4]. Pharmacokinetics of IFX has, so far, been described by both one- and two-compartment models [5, 6], therefore both were assessed to determine which would best describe the data. Models were evaluated by comparing the objective function values (OFV) as well as goodness-of-fit diagnostic plots and visual predictive checks. A two-compartment model calling ADVAN3 TRANS3 subroutine with first order elimination was selected (OFV 600.03). The estimated values of parameters (with relative standard error) were as follows:

Clearance = 0.38 L/day (12.4 %)
Steady-state volume of distribution = 4.26 L (23.2 %)
Intercompartmental clearance = 0.16 L/day (the value was fixed)
Central volume of distribution= 1.05 L (the value was fixed)
Inter-individual variability on clearance = 0.37 (28 %)
Proportional error = 0.66 (8.6%)
Additive error = 0.93 (37.8%)
Compared to data available in the literature, the obtained value of IFX CL in our patient population was slightly higher, probably due to disease severity and inflammation status of patients. Therefore, further analysis of covariate effects and exposure-response relationship will be explored in further research.

Conclusion: These preliminary results suggest that patients with fistulising form of Crohn’s disease may have higher IFX clearance and since higher trough concentrations are associated with fistulae healing, dose increase and/or dosing interval shortening could be beneficial to achieve disease remission.



References:

[1]    Gecse K, Khanna R, Stoker J, Jenkins JT, Gabe S, Hahnloser D, D’Haens G. Fistulizing Crohn’s disease: Diagnosis and management. United European gastroenterology journal. 2013 Jun;1(3):206-13.
[2]    Gu B, Venkatesh K, Williams AJ, Ng W, Corte C, Gholamrezaei A, Ghaly S, Xuan W, Paramsothy S, Connor S. Higher infliximab and adalimumab trough levels are associated with fistula healing in patients with fistulising perianal Crohn’s disease. World journal of gastroenterology. 2022 Jun 6;28(23):2597.
[3]    Owen JS, Fiedler-Kelly J. Introduction to population pharmacokinetic/pharmacodynamic analysis with nonlinear mixed effects models. John Wiley & Sons; 2014 Jun 19.
[4]    Beal SL. Ways to fit a PK model with some data below the quantification limit. Journal of pharmacokinetics and pharmacodynamics. 2001 Oct 1;28(5):481.
[5]    Konecki C, Feliu C, Cazaubon Y, Giusti D, Tonye-Libyh M, Brixi H, Cadiot G, Biron A, Djerada Z. External evaluation of population pharmacokinetic models and Bayes-based dosing of infliximab. Pharmaceutics. 2021 Aug 3;13(8):1191.
[6]    Schräpel C, Kovar L, Selzer D, Hofmann U, Tran F, Reinisch W, Schwab M, Lehr T. External model performance evaluation of twelve infliximab population pharmacokinetic models in patients with inflammatory bowel disease. Pharmaceutics. 2021 Aug 31;13(9):1368.
C3  - Page. Abstracts of the Annual Meeting of the Population Approach Group in Europe
T1  - Population pharmacokinetic model of infliximab in patients with fistulising Crohn’s disease
UR  - https://hdl.handle.net/21.15107/rcub_farfar_4898
ER  - 

@conference{
author = "Homšek, Ana and Marković, Srđan and Kralj, Đorđe and Odanović, Olga and Svorcan, Petar and Jovanović, Marija and Vučićević, Katarina",
year = "2023",
abstract = "Introduction: Infliximab (IFX), anti-tumour necrosis factor alpha antibody, is widely used in the treatment of inflammatory bowel diseases. Patients with Crohn’s disease may develop a more complicated form which involves fistulae formation, most commonly perianal. IFX has been effective in inducing fistula closure and maintaining disease remission [1]. In order to achieve fistulae healing adequate trough concentrations should be achieved [2].

Objectives:

Obtaining PK parameters for patients with the fistulising disease form
Comparing the results to the ones available in the literature for Crohn’s disease
Methods: In a retrospective review of medical documentation (from 2013 to present), 42 patients (64% male, 18-65 years old) with fistulising Crohn’s disease were selected for analysis. IFX concentrations were measured just before dose administration (trough concentration) or earlier if the patient was experiencing severe disease symptoms. For the majority of patients concentration was measured at week 14, right before the first maintenance dose. Additional concentrations were obtained at scheduled check-ups or after patients experienced signs of relapse. After appropriate premedication, each patient received the calculated fixed dose based on their body weight (dose range was 200-800 mg) via two-hour infusion. The drug was administered according to the standard (week 0, 2 and 6) and accelerated induction (every two weeks) protocols, followed by maintenance therapy (every 4, 6 or 8 weeks). Population modelling approach was applied to characterize pharmacokinetic profile of IFX using NONMEM 7.3 software (ICON Development Solutions Inc., Dublin, Ireland) [3]. The obtained data were processed using Microsoft Excel and R software.

Results: After exclusion of concentrations over the upper limit of quantification (12 μg/mL), a total of 161 concentrations were analysed. IFX concentrations below lower limit of quantification were accounted for using the M5 method [4]. Pharmacokinetics of IFX has, so far, been described by both one- and two-compartment models [5, 6], therefore both were assessed to determine which would best describe the data. Models were evaluated by comparing the objective function values (OFV) as well as goodness-of-fit diagnostic plots and visual predictive checks. A two-compartment model calling ADVAN3 TRANS3 subroutine with first order elimination was selected (OFV 600.03). The estimated values of parameters (with relative standard error) were as follows:

Clearance = 0.38 L/day (12.4 %)
Steady-state volume of distribution = 4.26 L (23.2 %)
Intercompartmental clearance = 0.16 L/day (the value was fixed)
Central volume of distribution= 1.05 L (the value was fixed)
Inter-individual variability on clearance = 0.37 (28 %)
Proportional error = 0.66 (8.6%)
Additive error = 0.93 (37.8%)
Compared to data available in the literature, the obtained value of IFX CL in our patient population was slightly higher, probably due to disease severity and inflammation status of patients. Therefore, further analysis of covariate effects and exposure-response relationship will be explored in further research.

Conclusion: These preliminary results suggest that patients with fistulising form of Crohn’s disease may have higher IFX clearance and since higher trough concentrations are associated with fistulae healing, dose increase and/or dosing interval shortening could be beneficial to achieve disease remission.



References:

[1]    Gecse K, Khanna R, Stoker J, Jenkins JT, Gabe S, Hahnloser D, D’Haens G. Fistulizing Crohn’s disease: Diagnosis and management. United European gastroenterology journal. 2013 Jun;1(3):206-13.
[2]    Gu B, Venkatesh K, Williams AJ, Ng W, Corte C, Gholamrezaei A, Ghaly S, Xuan W, Paramsothy S, Connor S. Higher infliximab and adalimumab trough levels are associated with fistula healing in patients with fistulising perianal Crohn’s disease. World journal of gastroenterology. 2022 Jun 6;28(23):2597.
[3]    Owen JS, Fiedler-Kelly J. Introduction to population pharmacokinetic/pharmacodynamic analysis with nonlinear mixed effects models. John Wiley & Sons; 2014 Jun 19.
[4]    Beal SL. Ways to fit a PK model with some data below the quantification limit. Journal of pharmacokinetics and pharmacodynamics. 2001 Oct 1;28(5):481.
[5]    Konecki C, Feliu C, Cazaubon Y, Giusti D, Tonye-Libyh M, Brixi H, Cadiot G, Biron A, Djerada Z. External evaluation of population pharmacokinetic models and Bayes-based dosing of infliximab. Pharmaceutics. 2021 Aug 3;13(8):1191.
[6]    Schräpel C, Kovar L, Selzer D, Hofmann U, Tran F, Reinisch W, Schwab M, Lehr T. External model performance evaluation of twelve infliximab population pharmacokinetic models in patients with inflammatory bowel disease. Pharmaceutics. 2021 Aug 31;13(9):1368.",
journal = "Page. Abstracts of the Annual Meeting of the Population Approach Group in Europe",
title = "Population pharmacokinetic model of infliximab in patients with fistulising Crohn’s disease",
url = "https://hdl.handle.net/21.15107/rcub_farfar_4898"
}

Homšek, A., Marković, S., Kralj, Đ., Odanović, O., Svorcan, P., Jovanović, M.,& Vučićević, K.. (2023). Population pharmacokinetic model of infliximab in patients with fistulising Crohn’s disease. in Page. Abstracts of the Annual Meeting of the Population Approach Group in Europe.
https://hdl.handle.net/21.15107/rcub_farfar_4898

Homšek A, Marković S, Kralj Đ, Odanović O, Svorcan P, Jovanović M, Vučićević K. Population pharmacokinetic model of infliximab in patients with fistulising Crohn’s disease. in Page. Abstracts of the Annual Meeting of the Population Approach Group in Europe. 2023;.
https://hdl.handle.net/21.15107/rcub_farfar_4898 .

Homšek, Ana, Marković, Srđan, Kralj, Đorđe, Odanović, Olga, Svorcan, Petar, Jovanović, Marija, Vučićević, Katarina, "Population pharmacokinetic model of infliximab in patients with fistulising Crohn’s disease" in Page. Abstracts of the Annual Meeting of the Population Approach Group in Europe (2023),
https://hdl.handle.net/21.15107/rcub_farfar_4898 .

TY  - JOUR
AU  - Milosavljević, Miloš
AU  - Okanović, Milan
AU  - Cicvarić Kostić, Slavica
AU  - Jovanović, Marija
AU  - Radonić, Milenko
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4473
AB  - Banknotes and coins are some of the most frequently traded items in the world. Their current use, however, is unsustainable, and many countries are trying to digitalize their payment systems. The recent pandemic has accelerated this transition. Building on the Theory of Unintended Consequences, the aim of this article is to examine the influence of some pandemic-specific factors (in specific, hand sanitization, conspiracy theory mentality, and financial acumen) on the current and prospective use of e-payment. A particular aim of the study is to analyze these relationships in Serbia (as an example of a cash-centric society). The study is based on primary data gathered via a questionnaire. The questionnaire was designed for the purpose of this study. In total, the study examined N = 474 examinees. The results of this study confirm that the pandemic-induced variables are statistically significant predictors of e-payment use. In particular, hand sanitization, conspiracy mentality (reversely), and financial acumen positively affect current and prospective e-payment use.
PB  - MDPI
T2  - Sustainability
T1  - COVID-19 and Behavioral Factors of e-Payment Use: Evidence from Serbia
VL  - 15
IS  - 4
DO  - 10.3390/su15043188
ER  - 

@article{
author = "Milosavljević, Miloš and Okanović, Milan and Cicvarić Kostić, Slavica and Jovanović, Marija and Radonić, Milenko",
year = "2023",
abstract = "Banknotes and coins are some of the most frequently traded items in the world. Their current use, however, is unsustainable, and many countries are trying to digitalize their payment systems. The recent pandemic has accelerated this transition. Building on the Theory of Unintended Consequences, the aim of this article is to examine the influence of some pandemic-specific factors (in specific, hand sanitization, conspiracy theory mentality, and financial acumen) on the current and prospective use of e-payment. A particular aim of the study is to analyze these relationships in Serbia (as an example of a cash-centric society). The study is based on primary data gathered via a questionnaire. The questionnaire was designed for the purpose of this study. In total, the study examined N = 474 examinees. The results of this study confirm that the pandemic-induced variables are statistically significant predictors of e-payment use. In particular, hand sanitization, conspiracy mentality (reversely), and financial acumen positively affect current and prospective e-payment use.",
publisher = "MDPI",
journal = "Sustainability",
title = "COVID-19 and Behavioral Factors of e-Payment Use: Evidence from Serbia",
volume = "15",
number = "4",
doi = "10.3390/su15043188"
}

Milosavljević, M., Okanović, M., Cicvarić Kostić, S., Jovanović, M.,& Radonić, M.. (2023). COVID-19 and Behavioral Factors of e-Payment Use: Evidence from Serbia. in Sustainability
MDPI., 15(4).
https://doi.org/10.3390/su15043188

Milosavljević M, Okanović M, Cicvarić Kostić S, Jovanović M, Radonić M. COVID-19 and Behavioral Factors of e-Payment Use: Evidence from Serbia. in Sustainability. 2023;15(4).
doi:10.3390/su15043188 .

Milosavljević, Miloš, Okanović, Milan, Cicvarić Kostić, Slavica, Jovanović, Marija, Radonić, Milenko, "COVID-19 and Behavioral Factors of e-Payment Use: Evidence from Serbia" in Sustainability, 15, no. 4 (2023),
https://doi.org/10.3390/su15043188 . .

TY  - JOUR
AU  - Mirjanić-Azarić, Bosa
AU  - Pejić, Ivana
AU  - Mijić, Smiljana
AU  - Pejčić, Aleksandra
AU  - Đurđević-Svraka, Anita
AU  - Svraka, Dragan
AU  - Knežević, Darija
AU  - Milivojac, Tatjana
AU  - Bogavac-Stanojević, Nataša
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5025
AB  - Background: The pandemic of severe acute respiratory syndrome by coronavirus 2 (SARS-CoV-2) is a multi-system disease caused by a diffuse systemic process involving a complex interaction of the inflammatory, immunological and coagulative cascades. This study aims to identify the most effective biomarkers to predict poor outcome in intensive care unit (ICU) patients with severe COVID-19 disease. Methods: A single-centre retrospective observational study enrolled 69 deceased and 20 recovered patients treated in the ICU of the General Hospital Gradiska in the period from March 1, 2021. until April 1, 2022. We evaluated the leukocytes (WBC), lymphocytes (LYM), neutrophils (NEU), platelets (PLT), haemoglobin, neutrophil-lymphocyte ratio (NLR), platelet lymphocyte ratio (PLR), and systemic immune-inflammation index (SII). In addition, we evaluated the IL-6, ferritin, CRP, D-dimer, magnesium, bilirubin and lactate dehydrogenase. Results: Between deceased and recovered patients on admission to the ICU, there was a significant differencebetween the following parameters: WBC x10 9/L 11.50 (8.86–14.75) vs. 9.40 (5.90–11.90), p =0.026 ; NEU x10 9 /L 10.15 (7.81–12.74) vs. 8.60 (4.80–10.30) p=0.022 ; NLR 15.01 (10.60–24.33) vs. 9.45 (5.10– 14.90) p=0.02 ; SII 3712 (2240–6543) vs. 1949 (993–3720) p=0.003 . The magnesium level increased significantly over time in the patients who died, while the haemoglobin level and platelet count decreased. ROC analysis showed the following AUC values: WBC 0.774; NEU 0.781; NLR 0.786; SII 0.776; D-dimer 0.741, and bilirubin 0.713. Conclusion: In this retrospective study WBC, NEU, NLR, SII, D-dimer, and bilirubin determined at hospital admission had a high value in predicting death among patients with severe COVID-19.
AB  - Uvod: Pandemija teškog akutnog respiratornog sindroma koronavirusa 2 (SARS-CoV-2) je multisistemska bolest izazvana difuznim sistemskim procesom koji uključuje kompleksne interakcije inflamatornih, imunoloških i koagulacionih kaskadnih procesa. Cilj ove studije je da utvrdi najefikasnije biomarkere u proceni lošeg ishoda pacijenata u jedinici intenzivne nege (ICU) obolelih od teškog oblika bolesti COVID-19. Metode: Retrospektivna opservaciona studija je uključivala 69 preminulih i 20 preživelih pacijenata lečenih u ICU u Opštoj bolnici Gradiška u periodu od 01.03.2021. do 01.04.2022. godine. Procenjivane su vrednosti leukocita (WBC), limfocita (LYM), neutrofila (NEU), trombocita (PLT), hemoglobina, odnosa neutrofila i limfocita (NLR), odnosa trom bocita i limfocita (PLR), sistemskog inflama tornog indeksa (SII). Takođe, procenjivane su vrednosti inter leukina6, feritina, CRP, D-dimera, magnezijuma, bilirubina i aktivnost laktat dehidrogenaze. Rezultati: Prilikom prijema pacijenata u ICU postojala je značajna razlika u vrednostima sledećih parametara između preminulih i preživelih pacijenata: WBC x 109/L 11,50 (8,86-14,75) prema (vs.) 9,40 (5,90-11,90), p=0,026; NEU x109/L 10,15 (7,81-12,74) vs. 8,60 (4,80-10,30) p=0,022; NLR 15,01 (10,60-24,33) vs. 9,45 (5,10-14,90) p=0,02; SII 3712 (2240-6543) vs. 1949 (993-3720) p=0,003. Takođe, tokom vremena koncentracija magnezijuma je značajno rasla u grupi preminulih pacijenata, dok je koncentracija hemoglobina i broj trombocita opadao. ROC analiza je pokazala sledeće AUC vrednosti: WBC 0,774; NEU 0,781; NLR 0,786; SII 0,776; D-dimer 0,741 i bilirubin 0,713. Zaključak: U ovoj retrospektivnoj studiji WBC, NEU, NLR, SII, D-dimer i bilirubin, određeni pri prijemu u ICU, su imali visoku vrednost u predviđanju smrti između pacijenata s teškim COVID-19.

PB  - Society of Medical Biochemists of Serbia
T2  - Journal of Medical Biochemistry 2023, vol. 42, br. 3, str. 513-523
T1  - The predictive role of biochemical markers on outcomes of severe covid-19 patients admitted to intensive care unit
T1  - Uloga biohemijskih markera u predviđanju ishoda teško obolelih covid-19 pacijenata primljenih na jedinicu intenzivnog lečenja
VL  - 42
IS  - 3
SP  - 513
EP  - 523
DO  - 10.5937/jomb0-40641
ER  - 

@article{
author = "Mirjanić-Azarić, Bosa and Pejić, Ivana and Mijić, Smiljana and Pejčić, Aleksandra and Đurđević-Svraka, Anita and Svraka, Dragan and Knežević, Darija and Milivojac, Tatjana and Bogavac-Stanojević, Nataša",
year = "2023",
abstract = "Background: The pandemic of severe acute respiratory syndrome by coronavirus 2 (SARS-CoV-2) is a multi-system disease caused by a diffuse systemic process involving a complex interaction of the inflammatory, immunological and coagulative cascades. This study aims to identify the most effective biomarkers to predict poor outcome in intensive care unit (ICU) patients with severe COVID-19 disease. Methods: A single-centre retrospective observational study enrolled 69 deceased and 20 recovered patients treated in the ICU of the General Hospital Gradiska in the period from March 1, 2021. until April 1, 2022. We evaluated the leukocytes (WBC), lymphocytes (LYM), neutrophils (NEU), platelets (PLT), haemoglobin, neutrophil-lymphocyte ratio (NLR), platelet lymphocyte ratio (PLR), and systemic immune-inflammation index (SII). In addition, we evaluated the IL-6, ferritin, CRP, D-dimer, magnesium, bilirubin and lactate dehydrogenase. Results: Between deceased and recovered patients on admission to the ICU, there was a significant differencebetween the following parameters: WBC x10 9/L 11.50 (8.86–14.75) vs. 9.40 (5.90–11.90), p =0.026 ; NEU x10 9 /L 10.15 (7.81–12.74) vs. 8.60 (4.80–10.30) p=0.022 ; NLR 15.01 (10.60–24.33) vs. 9.45 (5.10– 14.90) p=0.02 ; SII 3712 (2240–6543) vs. 1949 (993–3720) p=0.003 . The magnesium level increased significantly over time in the patients who died, while the haemoglobin level and platelet count decreased. ROC analysis showed the following AUC values: WBC 0.774; NEU 0.781; NLR 0.786; SII 0.776; D-dimer 0.741, and bilirubin 0.713. Conclusion: In this retrospective study WBC, NEU, NLR, SII, D-dimer, and bilirubin determined at hospital admission had a high value in predicting death among patients with severe COVID-19., Uvod: Pandemija teškog akutnog respiratornog sindroma koronavirusa 2 (SARS-CoV-2) je multisistemska bolest izazvana difuznim sistemskim procesom koji uključuje kompleksne interakcije inflamatornih, imunoloških i koagulacionih kaskadnih procesa. Cilj ove studije je da utvrdi najefikasnije biomarkere u proceni lošeg ishoda pacijenata u jedinici intenzivne nege (ICU) obolelih od teškog oblika bolesti COVID-19. Metode: Retrospektivna opservaciona studija je uključivala 69 preminulih i 20 preživelih pacijenata lečenih u ICU u Opštoj bolnici Gradiška u periodu od 01.03.2021. do 01.04.2022. godine. Procenjivane su vrednosti leukocita (WBC), limfocita (LYM), neutrofila (NEU), trombocita (PLT), hemoglobina, odnosa neutrofila i limfocita (NLR), odnosa trom bocita i limfocita (PLR), sistemskog inflama tornog indeksa (SII). Takođe, procenjivane su vrednosti inter leukina6, feritina, CRP, D-dimera, magnezijuma, bilirubina i aktivnost laktat dehidrogenaze. Rezultati: Prilikom prijema pacijenata u ICU postojala je značajna razlika u vrednostima sledećih parametara između preminulih i preživelih pacijenata: WBC x 109/L 11,50 (8,86-14,75) prema (vs.) 9,40 (5,90-11,90), p=0,026; NEU x109/L 10,15 (7,81-12,74) vs. 8,60 (4,80-10,30) p=0,022; NLR 15,01 (10,60-24,33) vs. 9,45 (5,10-14,90) p=0,02; SII 3712 (2240-6543) vs. 1949 (993-3720) p=0,003. Takođe, tokom vremena koncentracija magnezijuma je značajno rasla u grupi preminulih pacijenata, dok je koncentracija hemoglobina i broj trombocita opadao. ROC analiza je pokazala sledeće AUC vrednosti: WBC 0,774; NEU 0,781; NLR 0,786; SII 0,776; D-dimer 0,741 i bilirubin 0,713. Zaključak: U ovoj retrospektivnoj studiji WBC, NEU, NLR, SII, D-dimer i bilirubin, određeni pri prijemu u ICU, su imali visoku vrednost u predviđanju smrti između pacijenata s teškim COVID-19.
",
publisher = "Society of Medical Biochemists of Serbia",
journal = "Journal of Medical Biochemistry 2023, vol. 42, br. 3, str. 513-523",
title = "The predictive role of biochemical markers on outcomes of severe covid-19 patients admitted to intensive care unit, Uloga biohemijskih markera u predviđanju ishoda teško obolelih covid-19 pacijenata primljenih na jedinicu intenzivnog lečenja",
volume = "42",
number = "3",
pages = "513-523",
doi = "10.5937/jomb0-40641"
}

Mirjanić-Azarić, B., Pejić, I., Mijić, S., Pejčić, A., Đurđević-Svraka, A., Svraka, D., Knežević, D., Milivojac, T.,& Bogavac-Stanojević, N.. (2023). The predictive role of biochemical markers on outcomes of severe covid-19 patients admitted to intensive care unit. in Journal of Medical Biochemistry 2023, vol. 42, br. 3, str. 513-523
Society of Medical Biochemists of Serbia., 42(3), 513-523.
https://doi.org/10.5937/jomb0-40641

Mirjanić-Azarić B, Pejić I, Mijić S, Pejčić A, Đurđević-Svraka A, Svraka D, Knežević D, Milivojac T, Bogavac-Stanojević N. The predictive role of biochemical markers on outcomes of severe covid-19 patients admitted to intensive care unit. in Journal of Medical Biochemistry 2023, vol. 42, br. 3, str. 513-523. 2023;42(3):513-523.
doi:10.5937/jomb0-40641 .

Mirjanić-Azarić, Bosa, Pejić, Ivana, Mijić, Smiljana, Pejčić, Aleksandra, Đurđević-Svraka, Anita, Svraka, Dragan, Knežević, Darija, Milivojac, Tatjana, Bogavac-Stanojević, Nataša, "The predictive role of biochemical markers on outcomes of severe covid-19 patients admitted to intensive care unit" in Journal of Medical Biochemistry 2023, vol. 42, br. 3, str. 513-523, 42, no. 3 (2023):513-523,
https://doi.org/10.5937/jomb0-40641 . .

TY  - JOUR
AU  - Đekić, Saška
AU  - Vekić, Jelena
AU  - Zeljković, Aleksandra
AU  - Kotur-Stevuljević, Jelena
AU  - Kafedžić, Srđan
AU  - Zdravković, Marija
AU  - Ilić, Ivan
AU  - Hinić, Saša
AU  - Cerović, Milivoje
AU  - Stefanović, Milica
AU  - Mihajlović, Marija
AU  - Nešković, Aleksandar
AU  - Bogavac-Stanojević, Nataša
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4843
AB  - The aim of this multicentric study was to assess the impacts of oxidative stress, inflam-
mation, and the presence of small, dense, low-density lipoproteins (sdLDL) on the antioxidative
function of high-density lipoprotein (HDL) subclasses and the distribution of paraoxonase-1 (PON1)
activity within HDL in patients with ST-segment elevation acute myocardial infarction (STEMI). In
69 STEMI patients and 67 healthy control subjects, the lipoproteins’ subclasses were separated using
polyacrylamide gradient (3–31%) gel electrophoresis. The relative proportion of sdLDL and each
HDL subclass was evaluated by measuring the areas under the peaks of densitometric scans. The
distribution of the relative proportion of PON1 activity within the HDL subclasses (pPON1 within
HDL) was estimated using the zymogram method. The STEMI patients had significantly lower
proportions of HDL2a and HDL3a subclasses (p = 0.001 and p < 0.001, respectively) and lower pPON1
within HDL3b (p = 0.006), as well as higher proportions of HDL3b and HDL3c subclasses (p = 0.013
and p < 0.001, respectively) and higher pPON1 within HDL2 than the controls. Independent positive
associations between sdLDL and pPON1 within HDL3a and between malondialdehyde (MDA) and
pPON1 within HDL2b were shown in the STEMI group. The increased oxidative stress and increased
proportion of sdLDL in STEMI are closely related to the compromised antioxidative function of small
HDL3 particles and the altered pPON1 within HDL.
PB  - MDPI
T2  - International Journal of Molecular Sciences
T1  - HDL Subclasses and the Distribution of Paraoxonase-1 Activity in Patients with ST-Segment Elevation Acute Myocardial Infarction
VL  - 24
IS  - 11
DO  - 10.3390/ijms24119384
ER  - 

@article{
author = "Đekić, Saška and Vekić, Jelena and Zeljković, Aleksandra and Kotur-Stevuljević, Jelena and Kafedžić, Srđan and Zdravković, Marija and Ilić, Ivan and Hinić, Saša and Cerović, Milivoje and Stefanović, Milica and Mihajlović, Marija and Nešković, Aleksandar and Bogavac-Stanojević, Nataša",
year = "2023",
abstract = "The aim of this multicentric study was to assess the impacts of oxidative stress, inflam-
mation, and the presence of small, dense, low-density lipoproteins (sdLDL) on the antioxidative
function of high-density lipoprotein (HDL) subclasses and the distribution of paraoxonase-1 (PON1)
activity within HDL in patients with ST-segment elevation acute myocardial infarction (STEMI). In
69 STEMI patients and 67 healthy control subjects, the lipoproteins’ subclasses were separated using
polyacrylamide gradient (3–31%) gel electrophoresis. The relative proportion of sdLDL and each
HDL subclass was evaluated by measuring the areas under the peaks of densitometric scans. The
distribution of the relative proportion of PON1 activity within the HDL subclasses (pPON1 within
HDL) was estimated using the zymogram method. The STEMI patients had significantly lower
proportions of HDL2a and HDL3a subclasses (p = 0.001 and p < 0.001, respectively) and lower pPON1
within HDL3b (p = 0.006), as well as higher proportions of HDL3b and HDL3c subclasses (p = 0.013
and p < 0.001, respectively) and higher pPON1 within HDL2 than the controls. Independent positive
associations between sdLDL and pPON1 within HDL3a and between malondialdehyde (MDA) and
pPON1 within HDL2b were shown in the STEMI group. The increased oxidative stress and increased
proportion of sdLDL in STEMI are closely related to the compromised antioxidative function of small
HDL3 particles and the altered pPON1 within HDL.",
publisher = "MDPI",
journal = "International Journal of Molecular Sciences",
title = "HDL Subclasses and the Distribution of Paraoxonase-1 Activity in Patients with ST-Segment Elevation Acute Myocardial Infarction",
volume = "24",
number = "11",
doi = "10.3390/ijms24119384"
}

Đekić, S., Vekić, J., Zeljković, A., Kotur-Stevuljević, J., Kafedžić, S., Zdravković, M., Ilić, I., Hinić, S., Cerović, M., Stefanović, M., Mihajlović, M., Nešković, A.,& Bogavac-Stanojević, N.. (2023). HDL Subclasses and the Distribution of Paraoxonase-1 Activity in Patients with ST-Segment Elevation Acute Myocardial Infarction. in International Journal of Molecular Sciences
MDPI., 24(11).
https://doi.org/10.3390/ijms24119384

Đekić S, Vekić J, Zeljković A, Kotur-Stevuljević J, Kafedžić S, Zdravković M, Ilić I, Hinić S, Cerović M, Stefanović M, Mihajlović M, Nešković A, Bogavac-Stanojević N. HDL Subclasses and the Distribution of Paraoxonase-1 Activity in Patients with ST-Segment Elevation Acute Myocardial Infarction. in International Journal of Molecular Sciences. 2023;24(11).
doi:10.3390/ijms24119384 .

Đekić, Saška, Vekić, Jelena, Zeljković, Aleksandra, Kotur-Stevuljević, Jelena, Kafedžić, Srđan, Zdravković, Marija, Ilić, Ivan, Hinić, Saša, Cerović, Milivoje, Stefanović, Milica, Mihajlović, Marija, Nešković, Aleksandar, Bogavac-Stanojević, Nataša, "HDL Subclasses and the Distribution of Paraoxonase-1 Activity in Patients with ST-Segment Elevation Acute Myocardial Infarction" in International Journal of Molecular Sciences, 24, no. 11 (2023),
https://doi.org/10.3390/ijms24119384 . .