TY  - JOUR
AU  - Pešić, Deniel
AU  - Đukić, Mirjana
AU  - Stanojević, Ivan
AU  - Živković, Vladimir
AU  - Bolevich, Sergey
AU  - Bolevich, Stefani
AU  - Jakovljević, Vladimir
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5559
AB  - Background: The influence of homeostatically regulated physiological processes, including cardiorespiratory fitness (VO2max), on the response to physical stressors such as acclimatisation and marching, remains understudied. We aimed to investigate the effects of summer and winter acclimatisation and marching on cortisol levels and blood lactate, to gain insight into the role of these physiological processes in the stress response. Methods: Two groups of young Europeans, classified as poor (PCF; n=9) and good physical condition (GCF; n=21), based on a VO2max threshold of 40 mL O2/ kg/min, underwent 2-h march (6–7 km/h) in winter (5 ∘C) and summer (32 ∘C). Commercial tests, UniCel DxI Access Cortisol assay and EKF Biosen Clinic/GP assay were used for cortisol and lactate blood measurements (morning samples and those taken immediately after marches), respectively. Results: Basal cortisol levels were significantly higher at 5 °C than at 32 °C (PCF group: P=0.0079; cortisol dropped after the march at 5 °C in both groups, but increased at 32 °C only in PCF. Basal lactate levels were higher at 32 °C only in the GCF group (compared to PCF, P=0.0014) and post-marching (compared to basal values, P=0.0002). Conclusion: Cold exposure elicits a more significant stress response, based on higher basal cortisol levels, in individuals with worse physical fitness. Anaerobe metabolism prevails in participants with better physical fitness exposed to high ambient temperature based on increased basal and post-marching lactate levels. The study is significant for individual training/performance optimisation as it indicates the association between physiological stress responses and individual physical fitness levels.
AB  - Uvod: Uticaj homeostatski regulisanih fiziolo{kih procesa, uklju~uju}i kardiorespiratornu kondiciju (VO2max), na odgovor na fizi~ke stresore poput aklimatizacije i mar{i- ranja, ostaje nedovoljno prou~en. Cilj istra`ivanja je bio i da se ispita uticaj aklimatizacije i mar{iranja tokom leta i zime na nivoe kortizola i laktoze u krvi, pru`aju}i uvid u ulogu ovih fiziolo{kih procesa u odgovoru na stres. Metode: Dve grupe mladih Evropljana, klasifikovane kao osobe sa slabom (PCF; n=9) i dobrom fizi~kom kondicijom (GCF; n=21), na osnovu praga VO2max od 40 mL O 2/kg/min, su bile izlo`ene dvosatnom mar{u (6–7 km/h) u dva navrata, zimi na 5 °C i leti, na 32 °C. U uzorcima krvi (jutarnji i neposredno nakon mar{a) su odre|ivani nivoi kortizola i laktata komercijalnim testovima, UniCel DxI Access Cortisol i EKF Biosen Clinic/GP. Rezultati: Bazalni nivoi kortizola su bili zna~ajno vi{i na 5 °C nego na 32 °C (u PCF grupi: P=0,0079); nakon mar{i- ranja na 5 °C kortizol opada u obe grupe a pove}an je na 32 °C samo u PCF grupi. Bazalni nivoi laktoze bili su vi{i na 32 °C samo u GCF grupi (porede}i sa PCF grupom, P=0,0014) i nakon mar{iranja (porede}i sa bazalnim vred- nostima, P=0,0002).
PB  - Society of Medical Biochemists of Serbia
T2  - Journal of Medical Biochemistry
T1  - Cardiorespiratory fitness mediates cortisol and lactate responses to winter and summer marches
T1  - Kardiorespiratorni fitnes i njegov značaj u reakcijama kortizola i laktata prilikom zimskih i letnjih marševa
VL  - 43
IS  - 1
SP  - 72
EP  - 85
DO  - 10.5937/jomb0-44369
ER  - 

@article{
author = "Pešić, Deniel and Đukić, Mirjana and Stanojević, Ivan and Živković, Vladimir and Bolevich, Sergey and Bolevich, Stefani and Jakovljević, Vladimir",
year = "2024",
abstract = "Background: The influence of homeostatically regulated physiological processes, including cardiorespiratory fitness (VO2max), on the response to physical stressors such as acclimatisation and marching, remains understudied. We aimed to investigate the effects of summer and winter acclimatisation and marching on cortisol levels and blood lactate, to gain insight into the role of these physiological processes in the stress response. Methods: Two groups of young Europeans, classified as poor (PCF; n=9) and good physical condition (GCF; n=21), based on a VO2max threshold of 40 mL O2/ kg/min, underwent 2-h march (6–7 km/h) in winter (5 ∘C) and summer (32 ∘C). Commercial tests, UniCel DxI Access Cortisol assay and EKF Biosen Clinic/GP assay were used for cortisol and lactate blood measurements (morning samples and those taken immediately after marches), respectively. Results: Basal cortisol levels were significantly higher at 5 °C than at 32 °C (PCF group: P=0.0079; cortisol dropped after the march at 5 °C in both groups, but increased at 32 °C only in PCF. Basal lactate levels were higher at 32 °C only in the GCF group (compared to PCF, P=0.0014) and post-marching (compared to basal values, P=0.0002). Conclusion: Cold exposure elicits a more significant stress response, based on higher basal cortisol levels, in individuals with worse physical fitness. Anaerobe metabolism prevails in participants with better physical fitness exposed to high ambient temperature based on increased basal and post-marching lactate levels. The study is significant for individual training/performance optimisation as it indicates the association between physiological stress responses and individual physical fitness levels., Uvod: Uticaj homeostatski regulisanih fiziolo{kih procesa, uklju~uju}i kardiorespiratornu kondiciju (VO2max), na odgovor na fizi~ke stresore poput aklimatizacije i mar{i- ranja, ostaje nedovoljno prou~en. Cilj istra`ivanja je bio i da se ispita uticaj aklimatizacije i mar{iranja tokom leta i zime na nivoe kortizola i laktoze u krvi, pru`aju}i uvid u ulogu ovih fiziolo{kih procesa u odgovoru na stres. Metode: Dve grupe mladih Evropljana, klasifikovane kao osobe sa slabom (PCF; n=9) i dobrom fizi~kom kondicijom (GCF; n=21), na osnovu praga VO2max od 40 mL O 2/kg/min, su bile izlo`ene dvosatnom mar{u (6–7 km/h) u dva navrata, zimi na 5 °C i leti, na 32 °C. U uzorcima krvi (jutarnji i neposredno nakon mar{a) su odre|ivani nivoi kortizola i laktata komercijalnim testovima, UniCel DxI Access Cortisol i EKF Biosen Clinic/GP. Rezultati: Bazalni nivoi kortizola su bili zna~ajno vi{i na 5 °C nego na 32 °C (u PCF grupi: P=0,0079); nakon mar{i- ranja na 5 °C kortizol opada u obe grupe a pove}an je na 32 °C samo u PCF grupi. Bazalni nivoi laktoze bili su vi{i na 32 °C samo u GCF grupi (porede}i sa PCF grupom, P=0,0014) i nakon mar{iranja (porede}i sa bazalnim vred- nostima, P=0,0002).",
publisher = "Society of Medical Biochemists of Serbia",
journal = "Journal of Medical Biochemistry",
title = "Cardiorespiratory fitness mediates cortisol and lactate responses to winter and summer marches, Kardiorespiratorni fitnes i njegov značaj u reakcijama kortizola i laktata prilikom zimskih i letnjih marševa",
volume = "43",
number = "1",
pages = "72-85",
doi = "10.5937/jomb0-44369"
}

Pešić, D., Đukić, M., Stanojević, I., Živković, V., Bolevich, S., Bolevich, S.,& Jakovljević, V.. (2024). Cardiorespiratory fitness mediates cortisol and lactate responses to winter and summer marches. in Journal of Medical Biochemistry
Society of Medical Biochemists of Serbia., 43(1), 72-85.
https://doi.org/10.5937/jomb0-44369

Pešić D, Đukić M, Stanojević I, Živković V, Bolevich S, Bolevich S, Jakovljević V. Cardiorespiratory fitness mediates cortisol and lactate responses to winter and summer marches. in Journal of Medical Biochemistry. 2024;43(1):72-85.
doi:10.5937/jomb0-44369 .

Pešić, Deniel, Đukić, Mirjana, Stanojević, Ivan, Živković, Vladimir, Bolevich, Sergey, Bolevich, Stefani, Jakovljević, Vladimir, "Cardiorespiratory fitness mediates cortisol and lactate responses to winter and summer marches" in Journal of Medical Biochemistry, 43, no. 1 (2024):72-85,
https://doi.org/10.5937/jomb0-44369 . .

TY  - JOUR
AU  - Ušjak, Ljuboš
AU  - Stojković, Dejan
AU  - Carević, Tamara
AU  - Milutinović, Violeta
AU  - Soković, Marina
AU  - Niketić, Marjan
AU  - Petrović, Silvana
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5417
AB  - Plants of the genus Prangos are intensively investigated as potential new sources of bioactive isolated products. In this work, the chemical composition of volatile constituents (essential oils and headspace volatiles) and dichloromethane extracts, as well as antimicrobial and antibiofilm activities of essential oils and MFDEs (methanol fractions of dichloromethane extracts) of Prangos trifida from Serbia, were investigated. Volatiles of roots, leaves, stems and fruits, and fatty acids and phytosterols in dichloromethane extracts of roots and fruits were analyzed by GC-FID-MS, whereas coumarins in MFDEs by LC–MS and some isolated coumarins by 1H-NMR. Minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations/minimum fungicidal concentrations (MBCs/MFCs) of essential oils and MFDEs were determined against 13 microorganisms. Antibiofilm activity was assessed against four microorganisms. Additionally, congo red and ergosterol binding assays were conducted to elucidate selected mechanisms of antibiofilm action in the case of Candida albicans. Total of 52 volatile constituents, 16 fatty acids, eight phytosterols and 10 coumarins were identified. Essential oils demonstrated significant activity, surpassing that of commercial food preservatives, against six tested molds from the Aspergillus, Penicillium and Trichoderma genera, as well as against bacteria Staphylococcus aureus and Bacillus cereus. Most of the oils strongly inhibited the formation of biofilms by S. aureus, Listeria monocytogenes and Escherichia coli. MFDEs exhibited noteworthy effects against B. cereus and the tested Aspergillus species, particularly A. niger, and significantly inhibited C. albicans biofilm formation. This inhibition was linked to a marked reduction in exopolysaccharide production, while antifungal mechanisms associated with ergosterol remained unaffected.
PB  - MDPI
T2  - Antibiotics
T1  - Chemical Analysis and Investigation of Antimicrobial and Antibiofilm Activities of Prangos trifida (Apiaceae)
VL  - 13
IS  - 1
SP  - 41
DO  - 10.3390/antibiotics13010041
ER  - 

@article{
author = "Ušjak, Ljuboš and Stojković, Dejan and Carević, Tamara and Milutinović, Violeta and Soković, Marina and Niketić, Marjan and Petrović, Silvana",
year = "2024",
abstract = "Plants of the genus Prangos are intensively investigated as potential new sources of bioactive isolated products. In this work, the chemical composition of volatile constituents (essential oils and headspace volatiles) and dichloromethane extracts, as well as antimicrobial and antibiofilm activities of essential oils and MFDEs (methanol fractions of dichloromethane extracts) of Prangos trifida from Serbia, were investigated. Volatiles of roots, leaves, stems and fruits, and fatty acids and phytosterols in dichloromethane extracts of roots and fruits were analyzed by GC-FID-MS, whereas coumarins in MFDEs by LC–MS and some isolated coumarins by 1H-NMR. Minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations/minimum fungicidal concentrations (MBCs/MFCs) of essential oils and MFDEs were determined against 13 microorganisms. Antibiofilm activity was assessed against four microorganisms. Additionally, congo red and ergosterol binding assays were conducted to elucidate selected mechanisms of antibiofilm action in the case of Candida albicans. Total of 52 volatile constituents, 16 fatty acids, eight phytosterols and 10 coumarins were identified. Essential oils demonstrated significant activity, surpassing that of commercial food preservatives, against six tested molds from the Aspergillus, Penicillium and Trichoderma genera, as well as against bacteria Staphylococcus aureus and Bacillus cereus. Most of the oils strongly inhibited the formation of biofilms by S. aureus, Listeria monocytogenes and Escherichia coli. MFDEs exhibited noteworthy effects against B. cereus and the tested Aspergillus species, particularly A. niger, and significantly inhibited C. albicans biofilm formation. This inhibition was linked to a marked reduction in exopolysaccharide production, while antifungal mechanisms associated with ergosterol remained unaffected.",
publisher = "MDPI",
journal = "Antibiotics",
title = "Chemical Analysis and Investigation of Antimicrobial and Antibiofilm Activities of Prangos trifida (Apiaceae)",
volume = "13",
number = "1",
pages = "41",
doi = "10.3390/antibiotics13010041"
}

Ušjak, L., Stojković, D., Carević, T., Milutinović, V., Soković, M., Niketić, M.,& Petrović, S.. (2024). Chemical Analysis and Investigation of Antimicrobial and Antibiofilm Activities of Prangos trifida (Apiaceae). in Antibiotics
MDPI., 13(1), 41.
https://doi.org/10.3390/antibiotics13010041

Ušjak L, Stojković D, Carević T, Milutinović V, Soković M, Niketić M, Petrović S. Chemical Analysis and Investigation of Antimicrobial and Antibiofilm Activities of Prangos trifida (Apiaceae). in Antibiotics. 2024;13(1):41.
doi:10.3390/antibiotics13010041 .

Ušjak, Ljuboš, Stojković, Dejan, Carević, Tamara, Milutinović, Violeta, Soković, Marina, Niketić, Marjan, Petrović, Silvana, "Chemical Analysis and Investigation of Antimicrobial and Antibiofilm Activities of Prangos trifida (Apiaceae)" in Antibiotics, 13, no. 1 (2024):41,
https://doi.org/10.3390/antibiotics13010041 . .

TY  - JOUR
AU  - Ušjak, Ljuboš
AU  - Niketić, Marjan
AU  - Petrović, Silvana
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5418
AB  - The aim of this work was to investigate the composition of essential oils isolated from fruits of Peucedanum longifolium, and Rhizomatophora aegopodioides (a species which was previously placed in the genus Peucedanum), as well as to compare the obtained results to those available for other previously investigated related species (including taxa which are also, according to some authors, excluded from the genus Peucedanum). Essential oils were obtained via hydrodistillation in a Clevenger-type apparatus and their composition was analyzed using GC-FID and GC-MS. To compare these data to those of previously investigated taxa, a chemometric approach was applied; the data were analyzed using multivariate statistical methods: non-metric multidimensional scaling (nMDS) and hierarchical cluster analysis. The most abundant in P. longifolium essential oil were monoterpenes (79.7%), mostly α-phellandrene (26.2%), β-phellandrene + limonene (21.0%) and myrcene (9.5%), followed by sesquiterpenes (18.3%), mostly germacrene B (9.5%). On the other hand, dominant in R. aegopodioides essential oil were non-terpenic aliphatic hydrocarbons (46.1%), mainly n-undecane (16.5%) and n-nonane (11.3%). In addition, this essential oil also contained a notable quantity of sesquiterpenes (25.1%), with (E)-sesquilavandulol being the most abundant (10.0%). The results of multivariate statistics revealed a clear separation of the essential oil composition of R. aegopodioides and P. longifolium, as well as of P. longifolium and P. officinale. The clustering of the samples of most of the taxa that do not belong to the Peucedanum in the narrow sense (sensu stricto) was also observed, which is in accordance with their recent inclusion in separate genera.
PB  - MDPI
T2  - Separations
T1  - Composition of Essential Oils from Fruits of Peucedanum longifolium and Rhizomatophora aegopodioides (Apiaceae) with Regard to Other Related Taxa—A Chemometric Approach
VL  - 11
IS  - 1
SP  - 14
DO  - 10.3390/separations11010014
ER  - 

@article{
author = "Ušjak, Ljuboš and Niketić, Marjan and Petrović, Silvana",
year = "2024",
abstract = "The aim of this work was to investigate the composition of essential oils isolated from fruits of Peucedanum longifolium, and Rhizomatophora aegopodioides (a species which was previously placed in the genus Peucedanum), as well as to compare the obtained results to those available for other previously investigated related species (including taxa which are also, according to some authors, excluded from the genus Peucedanum). Essential oils were obtained via hydrodistillation in a Clevenger-type apparatus and their composition was analyzed using GC-FID and GC-MS. To compare these data to those of previously investigated taxa, a chemometric approach was applied; the data were analyzed using multivariate statistical methods: non-metric multidimensional scaling (nMDS) and hierarchical cluster analysis. The most abundant in P. longifolium essential oil were monoterpenes (79.7%), mostly α-phellandrene (26.2%), β-phellandrene + limonene (21.0%) and myrcene (9.5%), followed by sesquiterpenes (18.3%), mostly germacrene B (9.5%). On the other hand, dominant in R. aegopodioides essential oil were non-terpenic aliphatic hydrocarbons (46.1%), mainly n-undecane (16.5%) and n-nonane (11.3%). In addition, this essential oil also contained a notable quantity of sesquiterpenes (25.1%), with (E)-sesquilavandulol being the most abundant (10.0%). The results of multivariate statistics revealed a clear separation of the essential oil composition of R. aegopodioides and P. longifolium, as well as of P. longifolium and P. officinale. The clustering of the samples of most of the taxa that do not belong to the Peucedanum in the narrow sense (sensu stricto) was also observed, which is in accordance with their recent inclusion in separate genera.",
publisher = "MDPI",
journal = "Separations",
title = "Composition of Essential Oils from Fruits of Peucedanum longifolium and Rhizomatophora aegopodioides (Apiaceae) with Regard to Other Related Taxa—A Chemometric Approach",
volume = "11",
number = "1",
pages = "14",
doi = "10.3390/separations11010014"
}

Ušjak, L., Niketić, M.,& Petrović, S.. (2024). Composition of Essential Oils from Fruits of Peucedanum longifolium and Rhizomatophora aegopodioides (Apiaceae) with Regard to Other Related Taxa—A Chemometric Approach. in Separations
MDPI., 11(1), 14.
https://doi.org/10.3390/separations11010014

Ušjak L, Niketić M, Petrović S. Composition of Essential Oils from Fruits of Peucedanum longifolium and Rhizomatophora aegopodioides (Apiaceae) with Regard to Other Related Taxa—A Chemometric Approach. in Separations. 2024;11(1):14.
doi:10.3390/separations11010014 .

Ušjak, Ljuboš, Niketić, Marjan, Petrović, Silvana, "Composition of Essential Oils from Fruits of Peucedanum longifolium and Rhizomatophora aegopodioides (Apiaceae) with Regard to Other Related Taxa—A Chemometric Approach" in Separations, 11, no. 1 (2024):14,
https://doi.org/10.3390/separations11010014 . .

TY  - JOUR
AU  - Filipić, Brankica
AU  - Ušjak, Dušan
AU  - Hrast Rambaher, Martina
AU  - Oljačić, Slavica
AU  - Milenković, Marina
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5574
AB  - Antimicrobial resistance is a global threat, leading to an alarming increase in the prevalence of bacterial infections that can no longer be treated with available antibiotics. The World Health Organization estimates that by 2050 up to 10 million deaths per year could be associated with antimicrobial resistance, which would equal the annual number of cancer deaths worldwide. To overcome this emerging crisis, novel anti-bacterial compounds are urgently needed. There are two possible approaches in the fight against bacterial infections: a) targeting structures within bacterial cells, similar to existing antibiotics; and/or b) targeting virulence factors rather than bacterial growth. Here, for the first time, we provide a comprehensive overview of the key steps in the evaluation of potential new anti-bacterial and/or anti-virulence compounds. The methods described in this review include: a) in silico methods for the evaluation of novel compounds; b) anti-bacterial assays (MIC, MBC, Time-kill); b) anti-virulence assays (anti-biofilm, anti-quorum sensing, anti-adhesion); and c) evaluation of safety aspects (cytotoxicity assay and Ames test). Overall, we provide a detailed description of the methods that are an essential tool for chemists, computational chemists, microbiologists, and toxicologists in the evaluation of potential novel antimicrobial compounds. These methods are cost-effective and have high predictive value. They are widely used in preclinical studies to identify new molecular candidates, for further investigation in animal and human trials.
PB  - Frontiers Media SA
T2  - Frontiers in Cellular and Infection Microbiology
T1  - Evaluation of novel compounds as anti-bacterial or anti-virulence agents
VL  - 14
DO  - 10.3389/fcimb.2024.1370062
ER  - 

@article{
author = "Filipić, Brankica and Ušjak, Dušan and Hrast Rambaher, Martina and Oljačić, Slavica and Milenković, Marina",
year = "2024",
abstract = "Antimicrobial resistance is a global threat, leading to an alarming increase in the prevalence of bacterial infections that can no longer be treated with available antibiotics. The World Health Organization estimates that by 2050 up to 10 million deaths per year could be associated with antimicrobial resistance, which would equal the annual number of cancer deaths worldwide. To overcome this emerging crisis, novel anti-bacterial compounds are urgently needed. There are two possible approaches in the fight against bacterial infections: a) targeting structures within bacterial cells, similar to existing antibiotics; and/or b) targeting virulence factors rather than bacterial growth. Here, for the first time, we provide a comprehensive overview of the key steps in the evaluation of potential new anti-bacterial and/or anti-virulence compounds. The methods described in this review include: a) in silico methods for the evaluation of novel compounds; b) anti-bacterial assays (MIC, MBC, Time-kill); b) anti-virulence assays (anti-biofilm, anti-quorum sensing, anti-adhesion); and c) evaluation of safety aspects (cytotoxicity assay and Ames test). Overall, we provide a detailed description of the methods that are an essential tool for chemists, computational chemists, microbiologists, and toxicologists in the evaluation of potential novel antimicrobial compounds. These methods are cost-effective and have high predictive value. They are widely used in preclinical studies to identify new molecular candidates, for further investigation in animal and human trials.",
publisher = "Frontiers Media SA",
journal = "Frontiers in Cellular and Infection Microbiology",
title = "Evaluation of novel compounds as anti-bacterial or anti-virulence agents",
volume = "14",
doi = "10.3389/fcimb.2024.1370062"
}

Filipić, B., Ušjak, D., Hrast Rambaher, M., Oljačić, S.,& Milenković, M.. (2024). Evaluation of novel compounds as anti-bacterial or anti-virulence agents. in Frontiers in Cellular and Infection Microbiology
Frontiers Media SA., 14.
https://doi.org/10.3389/fcimb.2024.1370062

Filipić B, Ušjak D, Hrast Rambaher M, Oljačić S, Milenković M. Evaluation of novel compounds as anti-bacterial or anti-virulence agents. in Frontiers in Cellular and Infection Microbiology. 2024;14.
doi:10.3389/fcimb.2024.1370062 .

Filipić, Brankica, Ušjak, Dušan, Hrast Rambaher, Martina, Oljačić, Slavica, Milenković, Marina, "Evaluation of novel compounds as anti-bacterial or anti-virulence agents" in Frontiers in Cellular and Infection Microbiology, 14 (2024),
https://doi.org/10.3389/fcimb.2024.1370062 . .

TY  - JOUR
AU  - Dobričić, Vladimir
AU  - Savić, Jelena
AU  - Tomašić, Tihomir
AU  - Durcik, Martina
AU  - Zidar, Nace
AU  - Peterlin-Mašič, Lucija
AU  - Ilaš, Janez
AU  - Kikelj, Danijel
AU  - Čudina, Olivera
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5431
AB  - Bacterial DNA gyrase and topoisomerase IV control the topological state of DNA during replication
and represent important antibacterial drug targets. To be successful as drug candidates, newly synthesized compounds must possess optimal lipophilicity, which enables efficient delivery to the site of action. In this study, retention behavior of twenty-three previously synthesized dual DNA gyrase and topoisomerase IV inhibitors was tested in RP-HPLC system, consisting of C8 column and acetonitrile/phosphate buffer (pH 5.5 and pH 7.4) mobile phase. logD was calculated at both pH values and the best correlation with logD was obtained for retention parameter φ0, indicating that this RP-HPLC system could be used as an alternative to the shake-flask determination of lipophilicity. Subsequent QSRR analysis revealed that intrinsic lipophilicity (logP) and molecular weight (bcutm13) have a positive, while solubility (bcutp3) has a negative influence on this  retention parameter.
PB  - Akadémiai Kiadó
T2  - Acta Chromatographica
T1  - High-performance liquid chromatography evaluation of lipophilicity and QSRR modeling of a series of dual DNA gyrase and topoisomerase IV inhibitors
VL  - 36
IS  - 1
SP  - 45
EP  - 51
DO  - 10.1556/1326.2022.01096
ER  - 

@article{
author = "Dobričić, Vladimir and Savić, Jelena and Tomašić, Tihomir and Durcik, Martina and Zidar, Nace and Peterlin-Mašič, Lucija and Ilaš, Janez and Kikelj, Danijel and Čudina, Olivera",
year = "2024",
abstract = "Bacterial DNA gyrase and topoisomerase IV control the topological state of DNA during replication
and represent important antibacterial drug targets. To be successful as drug candidates, newly synthesized compounds must possess optimal lipophilicity, which enables efficient delivery to the site of action. In this study, retention behavior of twenty-three previously synthesized dual DNA gyrase and topoisomerase IV inhibitors was tested in RP-HPLC system, consisting of C8 column and acetonitrile/phosphate buffer (pH 5.5 and pH 7.4) mobile phase. logD was calculated at both pH values and the best correlation with logD was obtained for retention parameter φ0, indicating that this RP-HPLC system could be used as an alternative to the shake-flask determination of lipophilicity. Subsequent QSRR analysis revealed that intrinsic lipophilicity (logP) and molecular weight (bcutm13) have a positive, while solubility (bcutp3) has a negative influence on this  retention parameter.",
publisher = "Akadémiai Kiadó",
journal = "Acta Chromatographica",
title = "High-performance liquid chromatography evaluation of lipophilicity and QSRR modeling of a series of dual DNA gyrase and topoisomerase IV inhibitors",
volume = "36",
number = "1",
pages = "45-51",
doi = "10.1556/1326.2022.01096"
}

Dobričić, V., Savić, J., Tomašić, T., Durcik, M., Zidar, N., Peterlin-Mašič, L., Ilaš, J., Kikelj, D.,& Čudina, O.. (2024). High-performance liquid chromatography evaluation of lipophilicity and QSRR modeling of a series of dual DNA gyrase and topoisomerase IV inhibitors. in Acta Chromatographica
Akadémiai Kiadó., 36(1), 45-51.
https://doi.org/10.1556/1326.2022.01096

Dobričić V, Savić J, Tomašić T, Durcik M, Zidar N, Peterlin-Mašič L, Ilaš J, Kikelj D, Čudina O. High-performance liquid chromatography evaluation of lipophilicity and QSRR modeling of a series of dual DNA gyrase and topoisomerase IV inhibitors. in Acta Chromatographica. 2024;36(1):45-51.
doi:10.1556/1326.2022.01096 .

Dobričić, Vladimir, Savić, Jelena, Tomašić, Tihomir, Durcik, Martina, Zidar, Nace, Peterlin-Mašič, Lucija, Ilaš, Janez, Kikelj, Danijel, Čudina, Olivera, "High-performance liquid chromatography evaluation of lipophilicity and QSRR modeling of a series of dual DNA gyrase and topoisomerase IV inhibitors" in Acta Chromatographica, 36, no. 1 (2024):45-51,
https://doi.org/10.1556/1326.2022.01096 . .

TY  - JOUR
AU  - Milošević, Tamara
AU  - Sopić, Miron
AU  - Vekić, Jelena
AU  - Guzonjić, Azra
AU  - Vujčić, Sanja
AU  - Pešić, Snežana
AU  - Miljković-Trailović, Milica
AU  - Naumović, Radomir
AU  - Kotur-Stevuljević, Jelena
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5414
AB  - Purpose: End-stage renal disease patients on chronic hemodialysis (HD) have a shortened life expectancy compared to the general population. The aim of this study was to evaluate a possible link between three new and emerging factors in renal pathophysiology: Klotho protein, telomere length in peripheral blood mononuclear cells (TL) and redox status parameters before HD (bHD) and after HD (aHD), and to test mortality prediction capability of these emerging parameters in a population of HD patients. Methods: The study included 130 adult patients with average age 66 (54–72), on HD (3 times per week; 4–5 h per session). Klotho level, TL, routine laboratory parameters, dialysis adequacy and redox status parameters: advanced oxidation protein products (AOPP), prooxidant–antioxidant balance (PAB), superoxide anion (O2.−), malondialdehyde (MDA), ischemia-modified albumin (IMA), total sulfhydryl group content (SHG), and superoxide dismutase (SOD) were determined. Results: Klotho concentration was significantly higher aHD; 68.2 (22.6–152.9) vs. bHD 64.2 (25.5–119.8) (p = 0.027). The observed increase in TL was not statistically significant. AOPP, PAB, SHG, and SOD activity were significantly increased aHD (p > 0.001). The patients with the highest mortality risk score (MRS) had significantly higher PAB bHD (p = 0.002). Significantly lower O2.− (p < 0.001), SHG content (p = 0.072), and IMA (p = 0.002) aHD were found in patients with the lowest MRS values. Principal component analysis revealed redox balance-Klotho factor as a significant predictor of high mortality risk (p = 0.014). Conclusion: Decreased Klotho and TL attrition as well as redox status disturbance could be connected with higher mortality rate in HD patients.
PB  - Springer Science and Business Media B.V.
T2  - International Urology and Nephrology
T1  - The influence of Klotho protein and prooxidant–antioxidant balance combination on the mortality of HD patients
VL  - 56
IS  - 2
SP  - 615
EP  - 623
DO  - 10.1007/s11255-023-03696-w
ER  - 

@article{
author = "Milošević, Tamara and Sopić, Miron and Vekić, Jelena and Guzonjić, Azra and Vujčić, Sanja and Pešić, Snežana and Miljković-Trailović, Milica and Naumović, Radomir and Kotur-Stevuljević, Jelena",
year = "2024",
abstract = "Purpose: End-stage renal disease patients on chronic hemodialysis (HD) have a shortened life expectancy compared to the general population. The aim of this study was to evaluate a possible link between three new and emerging factors in renal pathophysiology: Klotho protein, telomere length in peripheral blood mononuclear cells (TL) and redox status parameters before HD (bHD) and after HD (aHD), and to test mortality prediction capability of these emerging parameters in a population of HD patients. Methods: The study included 130 adult patients with average age 66 (54–72), on HD (3 times per week; 4–5 h per session). Klotho level, TL, routine laboratory parameters, dialysis adequacy and redox status parameters: advanced oxidation protein products (AOPP), prooxidant–antioxidant balance (PAB), superoxide anion (O2.−), malondialdehyde (MDA), ischemia-modified albumin (IMA), total sulfhydryl group content (SHG), and superoxide dismutase (SOD) were determined. Results: Klotho concentration was significantly higher aHD; 68.2 (22.6–152.9) vs. bHD 64.2 (25.5–119.8) (p = 0.027). The observed increase in TL was not statistically significant. AOPP, PAB, SHG, and SOD activity were significantly increased aHD (p > 0.001). The patients with the highest mortality risk score (MRS) had significantly higher PAB bHD (p = 0.002). Significantly lower O2.− (p < 0.001), SHG content (p = 0.072), and IMA (p = 0.002) aHD were found in patients with the lowest MRS values. Principal component analysis revealed redox balance-Klotho factor as a significant predictor of high mortality risk (p = 0.014). Conclusion: Decreased Klotho and TL attrition as well as redox status disturbance could be connected with higher mortality rate in HD patients.",
publisher = "Springer Science and Business Media B.V.",
journal = "International Urology and Nephrology",
title = "The influence of Klotho protein and prooxidant–antioxidant balance combination on the mortality of HD patients",
volume = "56",
number = "2",
pages = "615-623",
doi = "10.1007/s11255-023-03696-w"
}

Milošević, T., Sopić, M., Vekić, J., Guzonjić, A., Vujčić, S., Pešić, S., Miljković-Trailović, M., Naumović, R.,& Kotur-Stevuljević, J.. (2024). The influence of Klotho protein and prooxidant–antioxidant balance combination on the mortality of HD patients. in International Urology and Nephrology
Springer Science and Business Media B.V.., 56(2), 615-623.
https://doi.org/10.1007/s11255-023-03696-w

Milošević T, Sopić M, Vekić J, Guzonjić A, Vujčić S, Pešić S, Miljković-Trailović M, Naumović R, Kotur-Stevuljević J. The influence of Klotho protein and prooxidant–antioxidant balance combination on the mortality of HD patients. in International Urology and Nephrology. 2024;56(2):615-623.
doi:10.1007/s11255-023-03696-w .

Milošević, Tamara, Sopić, Miron, Vekić, Jelena, Guzonjić, Azra, Vujčić, Sanja, Pešić, Snežana, Miljković-Trailović, Milica, Naumović, Radomir, Kotur-Stevuljević, Jelena, "The influence of Klotho protein and prooxidant–antioxidant balance combination on the mortality of HD patients" in International Urology and Nephrology, 56, no. 2 (2024):615-623,
https://doi.org/10.1007/s11255-023-03696-w . .

TY  - JOUR
AU  - Ingelman-Sundberg, Magnus
AU  - Jukić, Marin
AU  - Bråten, Line Skute
AU  - Kringen, Marianne Kristiansen
AU  - Molden, Espen
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5300
AB  - We   read   with   great   interest   the  paper  by  Zubiaur  et  al.1  on  the   analysis   of   a   genotype–phenotype  relationship  of  the  CYP2C:TG haplotype. This study, including 225 patients receiving one  of  6  different  drugs  and  liver  pieces  from  135  children  (median   age   7   years),   is   in   contrast to 2 studies by Bråten et al. using in vivo data from 875 escitalopram-treated2  and  840  sertraline-treated3    Norwegian    patients,  respectively,  in  which  significantly increased rate (+20 to 25%) of CYP2C19-dependent metabolism    of    these    drugs    was   found   for   the   CYP2C:TGhaplotype.
PB  - John Wiley and Sons Inc
T2  - Clinical Pharmacology and Therapeutics
T1  - What is the Current Clinical Impact of the CYP2CTG Haplotype?
VL  - 115
IS  - 2
SP  - 183
EP  - 183
DO  - 10.1002/cpt.3094
ER  - 

@article{
author = "Ingelman-Sundberg, Magnus and Jukić, Marin and Bråten, Line Skute and Kringen, Marianne Kristiansen and Molden, Espen",
year = "2024",
abstract = "We   read   with   great   interest   the  paper  by  Zubiaur  et  al.1  on  the   analysis   of   a   genotype–phenotype  relationship  of  the  CYP2C:TG haplotype. This study, including 225 patients receiving one  of  6  different  drugs  and  liver  pieces  from  135  children  (median   age   7   years),   is   in   contrast to 2 studies by Bråten et al. using in vivo data from 875 escitalopram-treated2  and  840  sertraline-treated3    Norwegian    patients,  respectively,  in  which  significantly increased rate (+20 to 25%) of CYP2C19-dependent metabolism    of    these    drugs    was   found   for   the   CYP2C:TGhaplotype.",
publisher = "John Wiley and Sons Inc",
journal = "Clinical Pharmacology and Therapeutics",
title = "What is the Current Clinical Impact of the CYP2CTG Haplotype?",
volume = "115",
number = "2",
pages = "183-183",
doi = "10.1002/cpt.3094"
}

Ingelman-Sundberg, M., Jukić, M., Bråten, L. S., Kringen, M. K.,& Molden, E.. (2024). What is the Current Clinical Impact of the CYP2CTG Haplotype?. in Clinical Pharmacology and Therapeutics
John Wiley and Sons Inc., 115(2), 183-183.
https://doi.org/10.1002/cpt.3094

Ingelman-Sundberg M, Jukić M, Bråten LS, Kringen MK, Molden E. What is the Current Clinical Impact of the CYP2CTG Haplotype?. in Clinical Pharmacology and Therapeutics. 2024;115(2):183-183.
doi:10.1002/cpt.3094 .

Ingelman-Sundberg, Magnus, Jukić, Marin, Bråten, Line Skute, Kringen, Marianne Kristiansen, Molden, Espen, "What is the Current Clinical Impact of the CYP2CTG Haplotype?" in Clinical Pharmacology and Therapeutics, 115, no. 2 (2024):183-183,
https://doi.org/10.1002/cpt.3094 . .

TY  - JOUR
AU  - Sharmin, Dishary
AU  - Divović, Branka
AU  - Ping, Xingjie
AU  - Cerne, Rok
AU  - Smith, Jodi L.
AU  - Rezvanian, Sepideh
AU  - Mondal, Prithu
AU  - Michelle, Meyer Jean
AU  - Kiley, Molly E.
AU  - Arnold, Leggy A.
AU  - Mian, Md Yeunus
AU  - Pandey, Kamal P.
AU  - Jin, Xiaoming
AU  - Mitrović, Jelena
AU  - Đorović, Đorđe
AU  - Lippa, Arnold
AU  - Cook, James M.
AU  - Golani, Lalit K.
AU  - Scholze, Petra
AU  - Savić, Miroslav
AU  - Witkin, Jeffrey M.
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5505
AB  - KRM-II-81 (1) is an imidazodiazepine GABAA receptor (GABAAR) potentiator with broad antiseizure efficacy and a low sedative burden. A brominated analogue, DS-II-73 (5), was synthesized and pharmacologically characterized as a potential backup compound as KRM-II-81 moves forward into development. The synthesis from 2-amino-5-bromophenyl)(pyridin-2yl)methanone (6) was processed in five steps with an overall yield of 38% and without the need for a palladium catalyst. GABAAR binding occurred with a Ki of 150 nM, and only 3 of 41 screened binding sites produced inhibition ≥50% at 10 μM, and the potency to induce cytotoxicity was ≥240 mM. DS-II-73 was selective for α2/3/5- over that of α1-containing GABAARs. Oral exposure of plasma and brain of rats was more than sufficient to functionally impact GABAARs. Tonic convulsions in mice and lethality induced by pentylenetetrazol were suppressed by DS-II-73 after oral administration and latencies to clonic and tonic seizures were prolonged. Cortical slice preparations from a patient with pharmacoresistant epilepsy (mesial temporal lobe) showed decreases in the frequency of local field potentials by DS-II-73. As with KRM-II-81, the motor-impairing effects of DS-II-73 were low compared to diazepam. Molecular docking studies of DS-II-73 with the α1β3γ2L-configured GABAAR showed low interaction with α1His102 that is suggested as a potential molecular mechanism for its low sedative side effects. These findings support the viability of DS-II-73 as a backup molecule for its ethynyl analogue, KRM-II-81, with the human tissue data providing translational credibility.
PB  - American Chemical Society
T2  - ACS Chemical Neuroscience
T1  - New Imidazodiazepine Analogue, 5-(8-Bromo-6-(pyridin-2-yl)-4H-benzo[f]imidazo[1,5-a][1,4]diazepin-3-yl)oxazole, Provides a Simplified Synthetic Scheme, High Oral Plasma and Brain Exposures, and Produces Antiseizure Efficacy in Mice, and Antiepileptogenic Activity in Neural Networks in Brain Slices from a Patient with Mesial Temporal Lobe Epilepsy
VL  - 15
IS  - 3
SP  - 517
EP  - 526
DO  - 10.1021/acschemneuro.3c00555
ER  - 

@article{
author = "Sharmin, Dishary and Divović, Branka and Ping, Xingjie and Cerne, Rok and Smith, Jodi L. and Rezvanian, Sepideh and Mondal, Prithu and Michelle, Meyer Jean and Kiley, Molly E. and Arnold, Leggy A. and Mian, Md Yeunus and Pandey, Kamal P. and Jin, Xiaoming and Mitrović, Jelena and Đorović, Đorđe and Lippa, Arnold and Cook, James M. and Golani, Lalit K. and Scholze, Petra and Savić, Miroslav and Witkin, Jeffrey M.",
year = "2024",
abstract = "KRM-II-81 (1) is an imidazodiazepine GABAA receptor (GABAAR) potentiator with broad antiseizure efficacy and a low sedative burden. A brominated analogue, DS-II-73 (5), was synthesized and pharmacologically characterized as a potential backup compound as KRM-II-81 moves forward into development. The synthesis from 2-amino-5-bromophenyl)(pyridin-2yl)methanone (6) was processed in five steps with an overall yield of 38% and without the need for a palladium catalyst. GABAAR binding occurred with a Ki of 150 nM, and only 3 of 41 screened binding sites produced inhibition ≥50% at 10 μM, and the potency to induce cytotoxicity was ≥240 mM. DS-II-73 was selective for α2/3/5- over that of α1-containing GABAARs. Oral exposure of plasma and brain of rats was more than sufficient to functionally impact GABAARs. Tonic convulsions in mice and lethality induced by pentylenetetrazol were suppressed by DS-II-73 after oral administration and latencies to clonic and tonic seizures were prolonged. Cortical slice preparations from a patient with pharmacoresistant epilepsy (mesial temporal lobe) showed decreases in the frequency of local field potentials by DS-II-73. As with KRM-II-81, the motor-impairing effects of DS-II-73 were low compared to diazepam. Molecular docking studies of DS-II-73 with the α1β3γ2L-configured GABAAR showed low interaction with α1His102 that is suggested as a potential molecular mechanism for its low sedative side effects. These findings support the viability of DS-II-73 as a backup molecule for its ethynyl analogue, KRM-II-81, with the human tissue data providing translational credibility.",
publisher = "American Chemical Society",
journal = "ACS Chemical Neuroscience",
title = "New Imidazodiazepine Analogue, 5-(8-Bromo-6-(pyridin-2-yl)-4H-benzo[f]imidazo[1,5-a][1,4]diazepin-3-yl)oxazole, Provides a Simplified Synthetic Scheme, High Oral Plasma and Brain Exposures, and Produces Antiseizure Efficacy in Mice, and Antiepileptogenic Activity in Neural Networks in Brain Slices from a Patient with Mesial Temporal Lobe Epilepsy",
volume = "15",
number = "3",
pages = "517-526",
doi = "10.1021/acschemneuro.3c00555"
}

Sharmin, D., Divović, B., Ping, X., Cerne, R., Smith, J. L., Rezvanian, S., Mondal, P., Michelle, M. J., Kiley, M. E., Arnold, L. A., Mian, M. Y., Pandey, K. P., Jin, X., Mitrović, J., Đorović, Đ., Lippa, A., Cook, J. M., Golani, L. K., Scholze, P., Savić, M.,& Witkin, J. M.. (2024). New Imidazodiazepine Analogue, 5-(8-Bromo-6-(pyridin-2-yl)-4H-benzo[f]imidazo[1,5-a][1,4]diazepin-3-yl)oxazole, Provides a Simplified Synthetic Scheme, High Oral Plasma and Brain Exposures, and Produces Antiseizure Efficacy in Mice, and Antiepileptogenic Activity in Neural Networks in Brain Slices from a Patient with Mesial Temporal Lobe Epilepsy. in ACS Chemical Neuroscience
American Chemical Society., 15(3), 517-526.
https://doi.org/10.1021/acschemneuro.3c00555

Sharmin D, Divović B, Ping X, Cerne R, Smith JL, Rezvanian S, Mondal P, Michelle MJ, Kiley ME, Arnold LA, Mian MY, Pandey KP, Jin X, Mitrović J, Đorović Đ, Lippa A, Cook JM, Golani LK, Scholze P, Savić M, Witkin JM. New Imidazodiazepine Analogue, 5-(8-Bromo-6-(pyridin-2-yl)-4H-benzo[f]imidazo[1,5-a][1,4]diazepin-3-yl)oxazole, Provides a Simplified Synthetic Scheme, High Oral Plasma and Brain Exposures, and Produces Antiseizure Efficacy in Mice, and Antiepileptogenic Activity in Neural Networks in Brain Slices from a Patient with Mesial Temporal Lobe Epilepsy. in ACS Chemical Neuroscience. 2024;15(3):517-526.
doi:10.1021/acschemneuro.3c00555 .

Sharmin, Dishary, Divović, Branka, Ping, Xingjie, Cerne, Rok, Smith, Jodi L., Rezvanian, Sepideh, Mondal, Prithu, Michelle, Meyer Jean, Kiley, Molly E., Arnold, Leggy A., Mian, Md Yeunus, Pandey, Kamal P., Jin, Xiaoming, Mitrović, Jelena, Đorović, Đorđe, Lippa, Arnold, Cook, James M., Golani, Lalit K., Scholze, Petra, Savić, Miroslav, Witkin, Jeffrey M., "New Imidazodiazepine Analogue, 5-(8-Bromo-6-(pyridin-2-yl)-4H-benzo[f]imidazo[1,5-a][1,4]diazepin-3-yl)oxazole, Provides a Simplified Synthetic Scheme, High Oral Plasma and Brain Exposures, and Produces Antiseizure Efficacy in Mice, and Antiepileptogenic Activity in Neural Networks in Brain Slices from a Patient with Mesial Temporal Lobe Epilepsy" in ACS Chemical Neuroscience, 15, no. 3 (2024):517-526,
https://doi.org/10.1021/acschemneuro.3c00555 . .

TY  - JOUR
AU  - Milovanović, Vera
AU  - Topić, Aleksandra
AU  - Milinković, Neda
AU  - Lazić, Zorica
AU  - Ivošević, Anita
AU  - Radojković, Dragica
AU  - Divac Rankov, Aleksandra
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4003
AB  - Objective: Chronic obstructive pulmonary disease (COPD) is multi-factorial disorder which
results from environmental influences and genetic factors. We aimed to investigate whether
methionine sulfoxide reductase A (MSRA) rs10903323 gene polymorphism is associated with
COPD development and severity in Serbian adult population.
Methods: The study included 155 patients with COPD and 134 healthy volunteers. Genotyping
was determined performing home-made polymerase chain reaction-restriction fragment length
polymorphism (PCR-RFLP). The difference between the inhibitory activities of normal and oxi-
dized Alpha-1-Antitrypsin (A1AT) against elastase and trypsin was used for determination of Oxi-
dized Alpha-1-Antitrypsin (OxyA1AT) (expressed as % and g/L). Functional activity of A1AT was
presented as a specific inhibitor activity to elastase (SIA-Elastase, kU/g).
Results: Frequencies of the genotypes AA, AG and GG were 80.0%, 20.0%, 0% in COPD patients
and 80.5%, 18.5% and 1.5% in the control group, and there was no significant difference in geno-
type or allele distributions between groups. Serum level of A1AT (g/L) and OxyA1AT was signifi-
cantly higher in COPD patients than in the control group, but functional activity of A1AT (SIA-
Elastase) was significantly lower in COPD patients than in the control group. In COPD group,
increased level of OxyA1ATwas present in G allele carriers who were smokers relative to G allele
carriers who were not smokers. In the smoker group of patients with severe and very severe
COPD (GOLD3+4), significant increase in OxyA1AT level was present in G allele carriers compared to AA homozygotes.
Conclusion: These findings suggest that MSRA rs10903323 gene polymorphism is probably not a
risk for COPD by itself but could represent a COPD modifier, since minor, G allele, is associated with an increased level of oxidized A1AT, indicating impaired ability of MSRA to repair oxidized
A1AT in COPD-smokers, and in severe form of COPD.
PB  - Elsevier
T2  - Pulmonology
T1  - Association of the methionine sulfoxide reductase A rs10903323 gene polymorphism with functional activity and oxidative modification of alpha-1-antitrypsin in COPD patients
VL  - 30
IS  - 2
SP  - 122
EP  - 129
DO  - 10.1016/j.pulmoe.2021.09.003
ER  - 

@article{
author = "Milovanović, Vera and Topić, Aleksandra and Milinković, Neda and Lazić, Zorica and Ivošević, Anita and Radojković, Dragica and Divac Rankov, Aleksandra",
year = "2024",
abstract = "Objective: Chronic obstructive pulmonary disease (COPD) is multi-factorial disorder which
results from environmental influences and genetic factors. We aimed to investigate whether
methionine sulfoxide reductase A (MSRA) rs10903323 gene polymorphism is associated with
COPD development and severity in Serbian adult population.
Methods: The study included 155 patients with COPD and 134 healthy volunteers. Genotyping
was determined performing home-made polymerase chain reaction-restriction fragment length
polymorphism (PCR-RFLP). The difference between the inhibitory activities of normal and oxi-
dized Alpha-1-Antitrypsin (A1AT) against elastase and trypsin was used for determination of Oxi-
dized Alpha-1-Antitrypsin (OxyA1AT) (expressed as % and g/L). Functional activity of A1AT was
presented as a specific inhibitor activity to elastase (SIA-Elastase, kU/g).
Results: Frequencies of the genotypes AA, AG and GG were 80.0%, 20.0%, 0% in COPD patients
and 80.5%, 18.5% and 1.5% in the control group, and there was no significant difference in geno-
type or allele distributions between groups. Serum level of A1AT (g/L) and OxyA1AT was signifi-
cantly higher in COPD patients than in the control group, but functional activity of A1AT (SIA-
Elastase) was significantly lower in COPD patients than in the control group. In COPD group,
increased level of OxyA1ATwas present in G allele carriers who were smokers relative to G allele
carriers who were not smokers. In the smoker group of patients with severe and very severe
COPD (GOLD3+4), significant increase in OxyA1AT level was present in G allele carriers compared to AA homozygotes.
Conclusion: These findings suggest that MSRA rs10903323 gene polymorphism is probably not a
risk for COPD by itself but could represent a COPD modifier, since minor, G allele, is associated with an increased level of oxidized A1AT, indicating impaired ability of MSRA to repair oxidized
A1AT in COPD-smokers, and in severe form of COPD.",
publisher = "Elsevier",
journal = "Pulmonology",
title = "Association of the methionine sulfoxide reductase A rs10903323 gene polymorphism with functional activity and oxidative modification of alpha-1-antitrypsin in COPD patients",
volume = "30",
number = "2",
pages = "122-129",
doi = "10.1016/j.pulmoe.2021.09.003"
}

Milovanović, V., Topić, A., Milinković, N., Lazić, Z., Ivošević, A., Radojković, D.,& Divac Rankov, A.. (2024). Association of the methionine sulfoxide reductase A rs10903323 gene polymorphism with functional activity and oxidative modification of alpha-1-antitrypsin in COPD patients. in Pulmonology
Elsevier., 30(2), 122-129.
https://doi.org/10.1016/j.pulmoe.2021.09.003

Milovanović V, Topić A, Milinković N, Lazić Z, Ivošević A, Radojković D, Divac Rankov A. Association of the methionine sulfoxide reductase A rs10903323 gene polymorphism with functional activity and oxidative modification of alpha-1-antitrypsin in COPD patients. in Pulmonology. 2024;30(2):122-129.
doi:10.1016/j.pulmoe.2021.09.003 .

Milovanović, Vera, Topić, Aleksandra, Milinković, Neda, Lazić, Zorica, Ivošević, Anita, Radojković, Dragica, Divac Rankov, Aleksandra, "Association of the methionine sulfoxide reductase A rs10903323 gene polymorphism with functional activity and oxidative modification of alpha-1-antitrypsin in COPD patients" in Pulmonology, 30, no. 2 (2024):122-129,
https://doi.org/10.1016/j.pulmoe.2021.09.003 . .

TY  - JOUR
AU  - Bjørklund, Geir
AU  - Buha-Đorđević, Aleksandra
AU  - Hamdan, Halla
AU  - Wallace, David R.
AU  - Peana, Massimiliano
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5443
AB  - Autoimmunity is a multifaceted disorder influenced by both genetic and environmental factors, and metal exposure has been implicated as a potential catalyst, especially in autoimmune diseases affecting the central nervous system. Notably, metals like mercury, lead, and aluminum exhibit well-established neurotoxic effects, yet the precise mechanisms by which they elicit autoimmune responses in susceptible individuals remain unclear. Recent studies propose that metal-induced autoimmunity may arise from direct toxic effects on immune cells and tissues, coupled with indirect impacts on the gut microbiome and the blood-brain barrier. These effects can activate self-reactive T cells, prompting the production of autoantibodies, inflammatory responses, and tissue damage. Diagnosing metal-induced autoimmunity proves challenging due to nonspecific symptoms and a lack of reliable biomarkers. Treatment typically involves chelation therapy to eliminate excess metals and immunomodulatory agents to suppress autoimmune responses. Prevention strategies include lifestyle adjustments to reduce metal exposure and avoiding occupational and environmental risks. Prognosis is generally favorable with proper treatment; however, untreated cases may lead to autoimmune disorder progression and irreversible organ damage, particularly in the brain. Future research aims to identify genetic and environmental risk factors, enhance diagnostic precision, and explore novel treatment approaches for improved prevention and management of this intricate and debilitating disease.
PB  - Elsevier B.V.
T2  - Autoimmunity Reviews
T1  - Metal-induced autoimmunity in neurological disorders: A review of current understanding and future directions
VL  - 23
IS  - 3
DO  - 10.1016/j.autrev.2023.103509
DO  - 2-s2.0-85181822086
ER  - 

@article{
author = "Bjørklund, Geir and Buha-Đorđević, Aleksandra and Hamdan, Halla and Wallace, David R. and Peana, Massimiliano",
year = "2024",
abstract = "Autoimmunity is a multifaceted disorder influenced by both genetic and environmental factors, and metal exposure has been implicated as a potential catalyst, especially in autoimmune diseases affecting the central nervous system. Notably, metals like mercury, lead, and aluminum exhibit well-established neurotoxic effects, yet the precise mechanisms by which they elicit autoimmune responses in susceptible individuals remain unclear. Recent studies propose that metal-induced autoimmunity may arise from direct toxic effects on immune cells and tissues, coupled with indirect impacts on the gut microbiome and the blood-brain barrier. These effects can activate self-reactive T cells, prompting the production of autoantibodies, inflammatory responses, and tissue damage. Diagnosing metal-induced autoimmunity proves challenging due to nonspecific symptoms and a lack of reliable biomarkers. Treatment typically involves chelation therapy to eliminate excess metals and immunomodulatory agents to suppress autoimmune responses. Prevention strategies include lifestyle adjustments to reduce metal exposure and avoiding occupational and environmental risks. Prognosis is generally favorable with proper treatment; however, untreated cases may lead to autoimmune disorder progression and irreversible organ damage, particularly in the brain. Future research aims to identify genetic and environmental risk factors, enhance diagnostic precision, and explore novel treatment approaches for improved prevention and management of this intricate and debilitating disease.",
publisher = "Elsevier B.V.",
journal = "Autoimmunity Reviews",
title = "Metal-induced autoimmunity in neurological disorders: A review of current understanding and future directions",
volume = "23",
number = "3",
doi = "10.1016/j.autrev.2023.103509, 2-s2.0-85181822086"
}

Bjørklund, G., Buha-Đorđević, A., Hamdan, H., Wallace, D. R.,& Peana, M.. (2024). Metal-induced autoimmunity in neurological disorders: A review of current understanding and future directions. in Autoimmunity Reviews
Elsevier B.V.., 23(3).
https://doi.org/10.1016/j.autrev.2023.103509

Bjørklund G, Buha-Đorđević A, Hamdan H, Wallace DR, Peana M. Metal-induced autoimmunity in neurological disorders: A review of current understanding and future directions. in Autoimmunity Reviews. 2024;23(3).
doi:10.1016/j.autrev.2023.103509 .

Bjørklund, Geir, Buha-Đorđević, Aleksandra, Hamdan, Halla, Wallace, David R., Peana, Massimiliano, "Metal-induced autoimmunity in neurological disorders: A review of current understanding and future directions" in Autoimmunity Reviews, 23, no. 3 (2024),
https://doi.org/10.1016/j.autrev.2023.103509 . .

TY  - JOUR
AU  - Sopić, Miron
AU  - Stopa, Victoria
AU  - Devaux, Yvan
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5432
PB  - Oxford University Press on behalf of the European Society of Cardiology
T2  - European Heart Journal
T1  - Leveraging epitranscriptomics for cardiovascular disease theranostics
VL  - 45
IS  - 13
SP  - 1098
EP  - 1100
DO  - 10.1093/eurheartj/ehad852
ER  - 

@article{
author = "Sopić, Miron and Stopa, Victoria and Devaux, Yvan",
year = "2024",
publisher = "Oxford University Press on behalf of the European Society of Cardiology",
journal = "European Heart Journal",
title = "Leveraging epitranscriptomics for cardiovascular disease theranostics",
volume = "45",
number = "13",
pages = "1098-1100",
doi = "10.1093/eurheartj/ehad852"
}

Sopić, M., Stopa, V.,& Devaux, Y.. (2024). Leveraging epitranscriptomics for cardiovascular disease theranostics. in European Heart Journal
Oxford University Press on behalf of the European Society of Cardiology., 45(13), 1098-1100.
https://doi.org/10.1093/eurheartj/ehad852

Sopić M, Stopa V, Devaux Y. Leveraging epitranscriptomics for cardiovascular disease theranostics. in European Heart Journal. 2024;45(13):1098-1100.
doi:10.1093/eurheartj/ehad852 .

Sopić, Miron, Stopa, Victoria, Devaux, Yvan, "Leveraging epitranscriptomics for cardiovascular disease theranostics" in European Heart Journal, 45, no. 13 (2024):1098-1100,
https://doi.org/10.1093/eurheartj/ehad852 . .

TY  - JOUR
AU  - Bruić, Marija
AU  - Pirković, Andrea
AU  - Borozan, Sunčica
AU  - Nacka-Aleksić, Mirjana
AU  - Jovanović-Krivokuća, Milica
AU  - Spremo-Potparević, Biljana
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5613
AB  - Oxidative stress has been implicated in numerous pregnancy-related disorders. Biologically active plant sec-
ondary metabolites, which are present in everyday diet, could prove effective therapeutic agents in preventing
these disorders. This study evaluated effects of taxifolin (dihydroquercetin) on ROS production, markers of
oxidative damage to lipids and proteins, activity of antioxidant enzymes and production of pro-inflammatory
cytokines in H2O2-induced oxidative stress in trophoblast HTR-8/SVneo cells. Taxifolin in 10 μM and 100 μM
concentrations attenuated oxidative damage to lipids and proteins, as evidenced by a decrease in MDA content,
extracellular LDH activity, carbonyl groups and nitrite contents. A reduction in the activity of antioxidant en-
zymes SOD, CAT and GPx in cells pre-treated with taxifolin, prior to H2O2 exposure, was also observed, along
with a reduction in intracellular ROS production. Both evaluated concentrations of taxifolin showed anti-
inflammatory activity in trophoblast cells, by reducing production of pro-inflammatory cytokines IL-1β and IL-
6. In this model of H2O2-induced oxidative stress, taxifolin showed marked antioxidative and anti-
inflammatory activities in trophoblast cells, adding further evidence of its protective effects and showing po-
tential as a therapeutic agent in preventing adverse pregnancy outcomes.
PB  - Elsevier
T2  - Reproductive Toxicology
T1  - Antioxidative and anti-inflammatory effects of taxifolin in H2O2-induced oxidative stress in HTR-8/SVneo trophoblast cell line
VL  - 126
SP  - 108585
DO  - 10.1016/j.reprotox.2024.108585
ER  - 

@article{
author = "Bruić, Marija and Pirković, Andrea and Borozan, Sunčica and Nacka-Aleksić, Mirjana and Jovanović-Krivokuća, Milica and Spremo-Potparević, Biljana",
year = "2024",
abstract = "Oxidative stress has been implicated in numerous pregnancy-related disorders. Biologically active plant sec-
ondary metabolites, which are present in everyday diet, could prove effective therapeutic agents in preventing
these disorders. This study evaluated effects of taxifolin (dihydroquercetin) on ROS production, markers of
oxidative damage to lipids and proteins, activity of antioxidant enzymes and production of pro-inflammatory
cytokines in H2O2-induced oxidative stress in trophoblast HTR-8/SVneo cells. Taxifolin in 10 μM and 100 μM
concentrations attenuated oxidative damage to lipids and proteins, as evidenced by a decrease in MDA content,
extracellular LDH activity, carbonyl groups and nitrite contents. A reduction in the activity of antioxidant en-
zymes SOD, CAT and GPx in cells pre-treated with taxifolin, prior to H2O2 exposure, was also observed, along
with a reduction in intracellular ROS production. Both evaluated concentrations of taxifolin showed anti-
inflammatory activity in trophoblast cells, by reducing production of pro-inflammatory cytokines IL-1β and IL-
6. In this model of H2O2-induced oxidative stress, taxifolin showed marked antioxidative and anti-
inflammatory activities in trophoblast cells, adding further evidence of its protective effects and showing po-
tential as a therapeutic agent in preventing adverse pregnancy outcomes.",
publisher = "Elsevier",
journal = "Reproductive Toxicology",
title = "Antioxidative and anti-inflammatory effects of taxifolin in H2O2-induced oxidative stress in HTR-8/SVneo trophoblast cell line",
volume = "126",
pages = "108585",
doi = "10.1016/j.reprotox.2024.108585"
}

Bruić, M., Pirković, A., Borozan, S., Nacka-Aleksić, M., Jovanović-Krivokuća, M.,& Spremo-Potparević, B.. (2024). Antioxidative and anti-inflammatory effects of taxifolin in H2O2-induced oxidative stress in HTR-8/SVneo trophoblast cell line. in Reproductive Toxicology
Elsevier., 126, 108585.
https://doi.org/10.1016/j.reprotox.2024.108585

Bruić M, Pirković A, Borozan S, Nacka-Aleksić M, Jovanović-Krivokuća M, Spremo-Potparević B. Antioxidative and anti-inflammatory effects of taxifolin in H2O2-induced oxidative stress in HTR-8/SVneo trophoblast cell line. in Reproductive Toxicology. 2024;126:108585.
doi:10.1016/j.reprotox.2024.108585 .

Bruić, Marija, Pirković, Andrea, Borozan, Sunčica, Nacka-Aleksić, Mirjana, Jovanović-Krivokuća, Milica, Spremo-Potparević, Biljana, "Antioxidative and anti-inflammatory effects of taxifolin in H2O2-induced oxidative stress in HTR-8/SVneo trophoblast cell line" in Reproductive Toxicology, 126 (2024):108585,
https://doi.org/10.1016/j.reprotox.2024.108585 . .

TY  - JOUR
AU  - Toljić, Mina
AU  - Nikolić, Nađa
AU  - Joksić, Ivana
AU  - Čarkić, Jelena
AU  - Munjas, Jelena
AU  - Karadžov Orlić, Nataša
AU  - Milašin, Jelena
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5615
AB  - Altered microRNAs (miRNAs1) and cytokines expression levels are associated with several pregnancy-induced complications. We evaluated the profile of circulating miRNAs (miR-17, miR-29a and miR-181a) and proinflammatory cytokines (TNF-α, IL-1β, IL-6 and IL-17) in women with gestational diabetes mellitus (GDM2), as well as their potential use as GDM biomarkers. The case-control study included 65 pregnant women divided into 2 groups - GDM and control. Expression levels of miRNAs in plasma samples and cytokines mRNA isolated from peripheral blood buffy coat were analyzed by quantitative real-time PCR (qPCR3). Significant miR-29a downregulation was found in GDM compared to the control group, and was even more significant after adjustments for covariates. miR-17 and miR-181a expression levels did not differ between the examined groups. Expression levels of IL-1β were significantly higher in GDM group compared to controls, while TNF-α, IL-6 and IL-17 did not show significant changes in expression between the two groups. As jugded from the ROC curve analysis, miR-29a and IL-1β had a significant capacity to discriminate between CG and GDM. Additionally, a positive correlation was established between IL-1β and TNF-α in the GDM group. GDM appeared to be associated with altered levels of miR-29a and IL-1β making them markers of this condition.
PB  - Elsevier B.V.
T2  - Journal of Reproductive Immunology
T1  - Expression of miRNAs and proinflammatory cytokines in pregnant women with gestational diabetes mellitus
VL  - 162
SP  - 104211
DO  - 10.1016/j.jri.2024.104211
ER  - 

@article{
author = "Toljić, Mina and Nikolić, Nađa and Joksić, Ivana and Čarkić, Jelena and Munjas, Jelena and Karadžov Orlić, Nataša and Milašin, Jelena",
year = "2024",
abstract = "Altered microRNAs (miRNAs1) and cytokines expression levels are associated with several pregnancy-induced complications. We evaluated the profile of circulating miRNAs (miR-17, miR-29a and miR-181a) and proinflammatory cytokines (TNF-α, IL-1β, IL-6 and IL-17) in women with gestational diabetes mellitus (GDM2), as well as their potential use as GDM biomarkers. The case-control study included 65 pregnant women divided into 2 groups - GDM and control. Expression levels of miRNAs in plasma samples and cytokines mRNA isolated from peripheral blood buffy coat were analyzed by quantitative real-time PCR (qPCR3). Significant miR-29a downregulation was found in GDM compared to the control group, and was even more significant after adjustments for covariates. miR-17 and miR-181a expression levels did not differ between the examined groups. Expression levels of IL-1β were significantly higher in GDM group compared to controls, while TNF-α, IL-6 and IL-17 did not show significant changes in expression between the two groups. As jugded from the ROC curve analysis, miR-29a and IL-1β had a significant capacity to discriminate between CG and GDM. Additionally, a positive correlation was established between IL-1β and TNF-α in the GDM group. GDM appeared to be associated with altered levels of miR-29a and IL-1β making them markers of this condition.",
publisher = "Elsevier B.V.",
journal = "Journal of Reproductive Immunology",
title = "Expression of miRNAs and proinflammatory cytokines in pregnant women with gestational diabetes mellitus",
volume = "162",
pages = "104211",
doi = "10.1016/j.jri.2024.104211"
}

Toljić, M., Nikolić, N., Joksić, I., Čarkić, J., Munjas, J., Karadžov Orlić, N.,& Milašin, J.. (2024). Expression of miRNAs and proinflammatory cytokines in pregnant women with gestational diabetes mellitus. in Journal of Reproductive Immunology
Elsevier B.V.., 162, 104211.
https://doi.org/10.1016/j.jri.2024.104211

Toljić M, Nikolić N, Joksić I, Čarkić J, Munjas J, Karadžov Orlić N, Milašin J. Expression of miRNAs and proinflammatory cytokines in pregnant women with gestational diabetes mellitus. in Journal of Reproductive Immunology. 2024;162:104211.
doi:10.1016/j.jri.2024.104211 .

Toljić, Mina, Nikolić, Nađa, Joksić, Ivana, Čarkić, Jelena, Munjas, Jelena, Karadžov Orlić, Nataša, Milašin, Jelena, "Expression of miRNAs and proinflammatory cytokines in pregnant women with gestational diabetes mellitus" in Journal of Reproductive Immunology, 162 (2024):104211,
https://doi.org/10.1016/j.jri.2024.104211 . .

TY  - JOUR
AU  - Račić, Anđelka
AU  - Jurišić-Dukovski, Bisera
AU  - Lovrić, Jasmina
AU  - Dobričić, Vladimir
AU  - Vučen, Sonja
AU  - Micov, Ana
AU  - Stepanović-Petrović, Radica
AU  - Tomić, Maja
AU  - Pecikoza, Uroš
AU  - Bajac, Jelena
AU  - Krajišnik, Danina
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5610
AB  - The incorporation of polymers into drug delivery vehicles has been shown to be a useful approach to prolong the
residence time of drugs in the precorneal tear film and to improve penetration into biological membranes. The
main objective of this research was to formulate novel viscous eye drops with ketotifen as the active ingredient,
containing the polysaccharides: chitosan (MCH), hydroxypropyl guar gum (HPG) and hyaluronic acid (SH) alone
and in combination as functional polymers. DSC and FT-IR techniques showed the compatibility between
ketotifen and polymers. Physicochemical and rheological analysis at ambient and simulated physiological conditions, as well as the evaluation of mucoadhesive properties showed that vehicles containing combinations of
polymers have suitable physicochemical and functional properties with demonstrated synergism between
combined polymers (MCH and HPG i.e. SH and HPG). The drug permeability was successfully estimated in vitro
using HCE-T cell-based models. MTT cytotoxicity assay demonstrates that the tested formulations were non-toxic
and well tolerated. In vivo preclinical study on mice revealed that both vehicles containing mixed polymers
enhanced and prolonged the antipruritic/analgesic-like effect of ophthalmic ketotifen. Based on these results,
both combinations of polysaccharide polymers, especially SH-HPG, could be considered as potential new carriers
for ketotifen for ophthalmic use.
PB  - Elsevier
T2  - International Journal of Pharmaceutics
T1  - Synergism of polysaccharide polymers in antihistamine eye drops: Influence on physicochemical properties and in vivo efficacy
VL  - 655
SP  - 124033
DO  - 10.1016/j.ijpharm.2024.124033
ER  - 

@article{
author = "Račić, Anđelka and Jurišić-Dukovski, Bisera and Lovrić, Jasmina and Dobričić, Vladimir and Vučen, Sonja and Micov, Ana and Stepanović-Petrović, Radica and Tomić, Maja and Pecikoza, Uroš and Bajac, Jelena and Krajišnik, Danina",
year = "2024",
abstract = "The incorporation of polymers into drug delivery vehicles has been shown to be a useful approach to prolong the
residence time of drugs in the precorneal tear film and to improve penetration into biological membranes. The
main objective of this research was to formulate novel viscous eye drops with ketotifen as the active ingredient,
containing the polysaccharides: chitosan (MCH), hydroxypropyl guar gum (HPG) and hyaluronic acid (SH) alone
and in combination as functional polymers. DSC and FT-IR techniques showed the compatibility between
ketotifen and polymers. Physicochemical and rheological analysis at ambient and simulated physiological conditions, as well as the evaluation of mucoadhesive properties showed that vehicles containing combinations of
polymers have suitable physicochemical and functional properties with demonstrated synergism between
combined polymers (MCH and HPG i.e. SH and HPG). The drug permeability was successfully estimated in vitro
using HCE-T cell-based models. MTT cytotoxicity assay demonstrates that the tested formulations were non-toxic
and well tolerated. In vivo preclinical study on mice revealed that both vehicles containing mixed polymers
enhanced and prolonged the antipruritic/analgesic-like effect of ophthalmic ketotifen. Based on these results,
both combinations of polysaccharide polymers, especially SH-HPG, could be considered as potential new carriers
for ketotifen for ophthalmic use.",
publisher = "Elsevier",
journal = "International Journal of Pharmaceutics",
title = "Synergism of polysaccharide polymers in antihistamine eye drops: Influence on physicochemical properties and in vivo efficacy",
volume = "655",
pages = "124033",
doi = "10.1016/j.ijpharm.2024.124033"
}

Račić, A., Jurišić-Dukovski, B., Lovrić, J., Dobričić, V., Vučen, S., Micov, A., Stepanović-Petrović, R., Tomić, M., Pecikoza, U., Bajac, J.,& Krajišnik, D.. (2024). Synergism of polysaccharide polymers in antihistamine eye drops: Influence on physicochemical properties and in vivo efficacy. in International Journal of Pharmaceutics
Elsevier., 655, 124033.
https://doi.org/10.1016/j.ijpharm.2024.124033

Račić A, Jurišić-Dukovski B, Lovrić J, Dobričić V, Vučen S, Micov A, Stepanović-Petrović R, Tomić M, Pecikoza U, Bajac J, Krajišnik D. Synergism of polysaccharide polymers in antihistamine eye drops: Influence on physicochemical properties and in vivo efficacy. in International Journal of Pharmaceutics. 2024;655:124033.
doi:10.1016/j.ijpharm.2024.124033 .

Račić, Anđelka, Jurišić-Dukovski, Bisera, Lovrić, Jasmina, Dobričić, Vladimir, Vučen, Sonja, Micov, Ana, Stepanović-Petrović, Radica, Tomić, Maja, Pecikoza, Uroš, Bajac, Jelena, Krajišnik, Danina, "Synergism of polysaccharide polymers in antihistamine eye drops: Influence on physicochemical properties and in vivo efficacy" in International Journal of Pharmaceutics, 655 (2024):124033,
https://doi.org/10.1016/j.ijpharm.2024.124033 . .

TY  - JOUR
AU  - Vasović, Dolika D.
AU  - Ivković, Sanja
AU  - Živanović, Ana
AU  - Major, Tamara
AU  - Milašin, Jelena M.
AU  - Nikolić, Nađa S.
AU  - Simonović, Jelena
AU  - Šutulović, Nikola
AU  - Hrnčić, Dragan
AU  - Stanojlović, Olivera
AU  - Vesković, Milena
AU  - Rašić, Dejan M.
AU  - Mladenović, Dušan
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5611
AB  - Diabetic retinopathy is not cured efficiently and changes of lifestyle measures may delay early retinal injury in
diabetes. The aim of our study was to investigate the effects of reduced daily light exposure on retinal vascular
changes in streptozotocin (STZ)-induced model of DM with emphasis on inflammation, Aqp4 expression, visual
cycle and cholesterol metabolism-related gene expression in rat retina and RPE. Male Wistar rats were divided
into the following groups: 1. control; 2. diabetic group (DM) treated with streptozotocin (100 mg/kg); 3. group
exposed to light/dark cycle 6/18 h (6/18); 4. diabetic group exposed to light/dark cycle 6/18 h (DM+6/18).
Retinal vascular abnormalities were estimated based on lectin staining, while the expression of genes involved in
the visual cycle, cholesterol metabolism, and inflammation was determined by qRT-PCR. Reduced light exposure
alleviated vasculopathy, gliosis and the expression of IL-1 and TNF-α in the retina with increased perivascular
Aqp4 expression. The expression of genes involved in visual cycle and cholesterol metabolism was significantly
up-regulated in RPE in DM+6/18 vs. DM group. In the retina only the expression of APOE was significantly
higher in DM+6/18 vs. DM group. Reduced light exposure mitigates vascular changes and gliosis in DM via its
anti-inflammatory effect, increased retinal cholesterol turnover and perivascular Aqp4 expression.
PB  - Elsevier
T2  - Chemico-Biological Interactions
T1  - Reduced light exposure mitigates streptozotocin-induced vascular changes and gliosis in diabetic retina by an anti-inflammatory effect and increased retinal cholesterol turnover
VL  - 394
SP  - 110996
DO  - 10.1016/j.cbi.2024.110996
ER  - 

@article{
author = "Vasović, Dolika D. and Ivković, Sanja and Živanović, Ana and Major, Tamara and Milašin, Jelena M. and Nikolić, Nađa S. and Simonović, Jelena and Šutulović, Nikola and Hrnčić, Dragan and Stanojlović, Olivera and Vesković, Milena and Rašić, Dejan M. and Mladenović, Dušan",
year = "2024",
abstract = "Diabetic retinopathy is not cured efficiently and changes of lifestyle measures may delay early retinal injury in
diabetes. The aim of our study was to investigate the effects of reduced daily light exposure on retinal vascular
changes in streptozotocin (STZ)-induced model of DM with emphasis on inflammation, Aqp4 expression, visual
cycle and cholesterol metabolism-related gene expression in rat retina and RPE. Male Wistar rats were divided
into the following groups: 1. control; 2. diabetic group (DM) treated with streptozotocin (100 mg/kg); 3. group
exposed to light/dark cycle 6/18 h (6/18); 4. diabetic group exposed to light/dark cycle 6/18 h (DM+6/18).
Retinal vascular abnormalities were estimated based on lectin staining, while the expression of genes involved in
the visual cycle, cholesterol metabolism, and inflammation was determined by qRT-PCR. Reduced light exposure
alleviated vasculopathy, gliosis and the expression of IL-1 and TNF-α in the retina with increased perivascular
Aqp4 expression. The expression of genes involved in visual cycle and cholesterol metabolism was significantly
up-regulated in RPE in DM+6/18 vs. DM group. In the retina only the expression of APOE was significantly
higher in DM+6/18 vs. DM group. Reduced light exposure mitigates vascular changes and gliosis in DM via its
anti-inflammatory effect, increased retinal cholesterol turnover and perivascular Aqp4 expression.",
publisher = "Elsevier",
journal = "Chemico-Biological Interactions",
title = "Reduced light exposure mitigates streptozotocin-induced vascular changes and gliosis in diabetic retina by an anti-inflammatory effect and increased retinal cholesterol turnover",
volume = "394",
pages = "110996",
doi = "10.1016/j.cbi.2024.110996"
}

Vasović, D. D., Ivković, S., Živanović, A., Major, T., Milašin, J. M., Nikolić, N. S., Simonović, J., Šutulović, N., Hrnčić, D., Stanojlović, O., Vesković, M., Rašić, D. M.,& Mladenović, D.. (2024). Reduced light exposure mitigates streptozotocin-induced vascular changes and gliosis in diabetic retina by an anti-inflammatory effect and increased retinal cholesterol turnover. in Chemico-Biological Interactions
Elsevier., 394, 110996.
https://doi.org/10.1016/j.cbi.2024.110996

Vasović DD, Ivković S, Živanović A, Major T, Milašin JM, Nikolić NS, Simonović J, Šutulović N, Hrnčić D, Stanojlović O, Vesković M, Rašić DM, Mladenović D. Reduced light exposure mitigates streptozotocin-induced vascular changes and gliosis in diabetic retina by an anti-inflammatory effect and increased retinal cholesterol turnover. in Chemico-Biological Interactions. 2024;394:110996.
doi:10.1016/j.cbi.2024.110996 .

Vasović, Dolika D., Ivković, Sanja, Živanović, Ana, Major, Tamara, Milašin, Jelena M., Nikolić, Nađa S., Simonović, Jelena, Šutulović, Nikola, Hrnčić, Dragan, Stanojlović, Olivera, Vesković, Milena, Rašić, Dejan M., Mladenović, Dušan, "Reduced light exposure mitigates streptozotocin-induced vascular changes and gliosis in diabetic retina by an anti-inflammatory effect and increased retinal cholesterol turnover" in Chemico-Biological Interactions, 394 (2024):110996,
https://doi.org/10.1016/j.cbi.2024.110996 . .

TY  - JOUR
AU  - Janošević-Ležaić, Aleksandra
AU  - Bajuk-Bogdanović, Danica
AU  - Ćirić-Marjanović, Gordana
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5612
AB  - In situ Raman spectroscopy was used for the first time to study the reaction intermediates/products of the
oxidative polymerization of aniline to polyaniline (PANI) with peroxydisulfate in aqueous solutions of different
initial acidity. Raman spectra were recorded for the drops of reaction mixture taken at various reaction times.
Five reaction systems with different pH of the monomer solution (pHmon), leading to 1-D nanostructured PANIs
(pHmon =5.8, 4.6 and 4.5) and granular PANIs (pHmon= 3.4 and 1.0) were explored. Spectral characteristics of
different reaction phases - athermal, exothermic and post-propagation were identified and correlated with the
proposed mechanisms of aniline polymerization and nanostructures formation. For the nanostructured PANIs,
the athermal phase was characterized by pronounced strengthening of the δ(N H)/ν(C––N)Q band at
1510–1520 cm 1, while the characteristic of the second exothermic phase was the strengthening of the ν(C~C)B/
ν(C~C)Phz band at c.a. 1636 cm 1 which becomes stronger than the ν(C––C)Q/ν(C~C)SQ band at c.a. 1590 cm–1.
For granular PANIs, the band at 1590 cm–1 was stronger than that at 1630 cm–1 for all reaction phases. For all
reaction systems, characteristic feature of the post-propagation phase was strengthening of the ν(C~N+•) band at
1330–1340 cm 1 due to delocalized polarons. For granular PANIs, the band associated with phenazine-like units
at c.a. 1415 cm 1 disappeared after the exothermic phase, while for nanostructured PANIs it was seen until the
end of the reaction. The obtained results are expected to be useful in further investigations of the intermediates/
products and mechanisms of polymerizations of other aromatic amines.
PB  - Elsevier
T2  - Synthetic Metals  Synthetic Metals
T1  - In situ Raman spectroscopy study of the oxidative polymerization of aniline in media of different acidity
VL  - 305
SP  - 117602
DO  - 10.1016/j.synthmet.2024.117602
ER  - 

@article{
author = "Janošević-Ležaić, Aleksandra and Bajuk-Bogdanović, Danica and Ćirić-Marjanović, Gordana",
year = "2024",
abstract = "In situ Raman spectroscopy was used for the first time to study the reaction intermediates/products of the
oxidative polymerization of aniline to polyaniline (PANI) with peroxydisulfate in aqueous solutions of different
initial acidity. Raman spectra were recorded for the drops of reaction mixture taken at various reaction times.
Five reaction systems with different pH of the monomer solution (pHmon), leading to 1-D nanostructured PANIs
(pHmon =5.8, 4.6 and 4.5) and granular PANIs (pHmon= 3.4 and 1.0) were explored. Spectral characteristics of
different reaction phases - athermal, exothermic and post-propagation were identified and correlated with the
proposed mechanisms of aniline polymerization and nanostructures formation. For the nanostructured PANIs,
the athermal phase was characterized by pronounced strengthening of the δ(N H)/ν(C––N)Q band at
1510–1520 cm 1, while the characteristic of the second exothermic phase was the strengthening of the ν(C~C)B/
ν(C~C)Phz band at c.a. 1636 cm 1 which becomes stronger than the ν(C––C)Q/ν(C~C)SQ band at c.a. 1590 cm–1.
For granular PANIs, the band at 1590 cm–1 was stronger than that at 1630 cm–1 for all reaction phases. For all
reaction systems, characteristic feature of the post-propagation phase was strengthening of the ν(C~N+•) band at
1330–1340 cm 1 due to delocalized polarons. For granular PANIs, the band associated with phenazine-like units
at c.a. 1415 cm 1 disappeared after the exothermic phase, while for nanostructured PANIs it was seen until the
end of the reaction. The obtained results are expected to be useful in further investigations of the intermediates/
products and mechanisms of polymerizations of other aromatic amines.",
publisher = "Elsevier",
journal = "Synthetic Metals  Synthetic Metals",
title = "In situ Raman spectroscopy study of the oxidative polymerization of aniline in media of different acidity",
volume = "305",
pages = "117602",
doi = "10.1016/j.synthmet.2024.117602"
}

Janošević-Ležaić, A., Bajuk-Bogdanović, D.,& Ćirić-Marjanović, G.. (2024). In situ Raman spectroscopy study of the oxidative polymerization of aniline in media of different acidity. in Synthetic Metals  Synthetic Metals
Elsevier., 305, 117602.
https://doi.org/10.1016/j.synthmet.2024.117602

Janošević-Ležaić A, Bajuk-Bogdanović D, Ćirić-Marjanović G. In situ Raman spectroscopy study of the oxidative polymerization of aniline in media of different acidity. in Synthetic Metals  Synthetic Metals. 2024;305:117602.
doi:10.1016/j.synthmet.2024.117602 .

Janošević-Ležaić, Aleksandra, Bajuk-Bogdanović, Danica, Ćirić-Marjanović, Gordana, "In situ Raman spectroscopy study of the oxidative polymerization of aniline in media of different acidity" in Synthetic Metals  Synthetic Metals, 305 (2024):117602,
https://doi.org/10.1016/j.synthmet.2024.117602 . .

TY  - CONF
AU  - Živković, Lada
AU  - Spremo-Potparević, Biljana
AU  - Bajić, Vladan
AU  - Brankov, Jovan
AU  - Zhou, Wei
AU  - Brey, Eric
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5608
AB  - INTRODUCTION: Imaging  techniques  based  on  X-ray  phase-contrast  (XPC)  have  shown  tremendous  promise  for applications involving biomaterials and soft tissue formation [1,2].XPC imaging can be applied at higher energy offering the  potential  for  lower  dose  imaging.  Essential  to  the  development  of  this  technique  and  its  routine  use  is  an understanding of the potential damage of X-ray dose on cells and tissues. EXPERIMENTAL: In this study the comet assay, a sensitive assay for DNA damage, was used to evaluate DNA damage on  mesenchymal  stem  cells  (MSCs) exposedto X-ray  irradiation.  We  examined  the  effects  of  early  (immediately following  irradiation)  and  delayed  (24h  post-irradiation) X-ray  effects  caused  by  low  (15mGy)  and  intermediate (150mGy  and  1.5  Gy)  exposure  on  MSCs  during  a  monitoring  period  of  4  weeks  (five  irradiations,  one  weekly).  Cells were submitted to apolychromatic X-ray source (Thermo Fisher PXS10 conditions: voltage 45 kV, source current 160A, source power 7.2 W, source spot size 9 um, photon flux on the sample 7.66106photonss-1mm-2irradiation).Statistical analysis was performedby using Two-way analysis of variance (ANOVA) with Tukey’s multiple comparisons posttest in GraphPad Prism 5.0.A difference at p< 0.05 was considered statistically significant.RESULTS AND DISCUSSION: Resultsof the DNA comet assay indicated that early effects of low-and intermediate-dose of XPC induced an increase in the number of cells with DNA damage after each irradiation, where intermediate-dose (150 mGy and 1.5 Gy) produced significantly higher damage relative to controls. DNA damage induced by low and intermediate doses returned to the control value 24h after the irradiation exposure, suggesting a strong protection of MSCs at the tested doses of XPC irradiation. CONCLUSIONS: The data presented in this studyshows that 24 h after the last of five weekly low and intermediate doses XPC irradiation, the harmful effects on DNA in MSCs were notdetected. The current study reinforces the need of investigating consequences of low and intermediate doses of X-ray PC irradiation in the field of tissue engineering and provide new basis for MSCs using in the clinics.
PB  - Savez hemijskih inženjera, Beograd
C3  - Hemijska industrija
T1  - Examination of the effects of X-ray phase contrast imaging dose on DNA in mesenchymal stem cells by comet assay: Abstract
VL  - 78
IS  - 1S
SP  - 14
EP  - 14
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5608
ER  - 

@conference{
author = "Živković, Lada and Spremo-Potparević, Biljana and Bajić, Vladan and Brankov, Jovan and Zhou, Wei and Brey, Eric",
year = "2024",
abstract = "INTRODUCTION: Imaging  techniques  based  on  X-ray  phase-contrast  (XPC)  have  shown  tremendous  promise  for applications involving biomaterials and soft tissue formation [1,2].XPC imaging can be applied at higher energy offering the  potential  for  lower  dose  imaging.  Essential  to  the  development  of  this  technique  and  its  routine  use  is  an understanding of the potential damage of X-ray dose on cells and tissues. EXPERIMENTAL: In this study the comet assay, a sensitive assay for DNA damage, was used to evaluate DNA damage on  mesenchymal  stem  cells  (MSCs) exposedto X-ray  irradiation.  We  examined  the  effects  of  early  (immediately following  irradiation)  and  delayed  (24h  post-irradiation) X-ray  effects  caused  by  low  (15mGy)  and  intermediate (150mGy  and  1.5  Gy)  exposure  on  MSCs  during  a  monitoring  period  of  4  weeks  (five  irradiations,  one  weekly).  Cells were submitted to apolychromatic X-ray source (Thermo Fisher PXS10 conditions: voltage 45 kV, source current 160A, source power 7.2 W, source spot size 9 um, photon flux on the sample 7.66106photonss-1mm-2irradiation).Statistical analysis was performedby using Two-way analysis of variance (ANOVA) with Tukey’s multiple comparisons posttest in GraphPad Prism 5.0.A difference at p< 0.05 was considered statistically significant.RESULTS AND DISCUSSION: Resultsof the DNA comet assay indicated that early effects of low-and intermediate-dose of XPC induced an increase in the number of cells with DNA damage after each irradiation, where intermediate-dose (150 mGy and 1.5 Gy) produced significantly higher damage relative to controls. DNA damage induced by low and intermediate doses returned to the control value 24h after the irradiation exposure, suggesting a strong protection of MSCs at the tested doses of XPC irradiation. CONCLUSIONS: The data presented in this studyshows that 24 h after the last of five weekly low and intermediate doses XPC irradiation, the harmful effects on DNA in MSCs were notdetected. The current study reinforces the need of investigating consequences of low and intermediate doses of X-ray PC irradiation in the field of tissue engineering and provide new basis for MSCs using in the clinics.",
publisher = "Savez hemijskih inženjera, Beograd",
journal = "Hemijska industrija",
title = "Examination of the effects of X-ray phase contrast imaging dose on DNA in mesenchymal stem cells by comet assay: Abstract",
volume = "78",
number = "1S",
pages = "14-14",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5608"
}

Živković, L., Spremo-Potparević, B., Bajić, V., Brankov, J., Zhou, W.,& Brey, E.. (2024). Examination of the effects of X-ray phase contrast imaging dose on DNA in mesenchymal stem cells by comet assay: Abstract. in Hemijska industrija
Savez hemijskih inženjera, Beograd., 78(1S), 14-14.
https://doi.org/10.1016/j.reprotox.2024.108585
https://hdl.handle.net/21.15107/rcub_farfar_5608

Živković L, Spremo-Potparević B, Bajić V, Brankov J, Zhou W, Brey E. Examination of the effects of X-ray phase contrast imaging dose on DNA in mesenchymal stem cells by comet assay: Abstract. in Hemijska industrija. 2024;78(1S):14-14.
doi:10.1016/j.reprotox.2024.108585
https://hdl.handle.net/21.15107/rcub_farfar_5608 .

Živković, Lada, Spremo-Potparević, Biljana, Bajić, Vladan, Brankov, Jovan, Zhou, Wei, Brey, Eric, "Examination of the effects of X-ray phase contrast imaging dose on DNA in mesenchymal stem cells by comet assay: Abstract" in Hemijska industrija, 78, no. 1S (2024):14-14,
https://doi.org/10.1016/j.reprotox.2024.108585 .,
https://hdl.handle.net/21.15107/rcub_farfar_5608 .

TY  - JOUR
AU  - Bužančić, Iva
AU  - Držaić, Margita
AU  - Kummer, Ingrid
AU  - Ortner Hadžiabdić, Maja
AU  - Brkić, Jovana
AU  - Fialová, Daniela
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5571
AB  - Pharmacist’s geriatric assessment can provide valuable insights into potential deprescribing targets, while including important information on various health-related domains. Data collected from a geriatric assessment questionnaire, for 388 patients, from the Croatian cohort of the EuroAgeism H2020 ESR 7 international project, along with guideline-based deprescribing criteria, were used to analyse potentially inappropriate prescribing of four medication groups (benzodiazepines (BZN), proton pump inhibitors (PPI), opioids, and non-steroidal anti-inflammatory drugs (NSAID)), and to assess the deprescribing potential. Binary logistic regression was used to explore the effects of age, gender, number of medicines and diagnoses, self-reported health, frailty score, and healthcare utilization on the likelihood of needing deprescribing. More than half of participants (n = 216, 55.2%) are candidates for deprescribing, with 31.1% of PPI, 74.8% of NSAID, 75% of opioid, and 96.1% of BZN users meeting at least one criterion. Most common criteria for deprescribing were inappropriately long use and safety concerns. Women (aOR = 2.58; p < 0.001), those reporting poor self-reported health (aOR = 5.14; p < 0.001), and those exposed to polypharmacy (aOR = 1.29; p < 0.001) had higher odds of needing to have medicines deprescribed. The high rate of deprescribing potential warrants prompt action to increase patient safety and decrease polypharmacy. Pharmacist’s geriatric assessment and deprescribing-focused medication review could be used to lead a personalised approach.
PB  - Nature Research
T2  - Scientific Reports
T1  - Deprescribing potential of commonly used medications among community-dwelling older adults: insights from a pharmacist’s geriatric assessment
VL  - 14
IS  - 1
SP  - 6235
DO  - 10.1038/s41598-024-56780-1
ER  - 

@article{
author = "Bužančić, Iva and Držaić, Margita and Kummer, Ingrid and Ortner Hadžiabdić, Maja and Brkić, Jovana and Fialová, Daniela",
year = "2024",
abstract = "Pharmacist’s geriatric assessment can provide valuable insights into potential deprescribing targets, while including important information on various health-related domains. Data collected from a geriatric assessment questionnaire, for 388 patients, from the Croatian cohort of the EuroAgeism H2020 ESR 7 international project, along with guideline-based deprescribing criteria, were used to analyse potentially inappropriate prescribing of four medication groups (benzodiazepines (BZN), proton pump inhibitors (PPI), opioids, and non-steroidal anti-inflammatory drugs (NSAID)), and to assess the deprescribing potential. Binary logistic regression was used to explore the effects of age, gender, number of medicines and diagnoses, self-reported health, frailty score, and healthcare utilization on the likelihood of needing deprescribing. More than half of participants (n = 216, 55.2%) are candidates for deprescribing, with 31.1% of PPI, 74.8% of NSAID, 75% of opioid, and 96.1% of BZN users meeting at least one criterion. Most common criteria for deprescribing were inappropriately long use and safety concerns. Women (aOR = 2.58; p < 0.001), those reporting poor self-reported health (aOR = 5.14; p < 0.001), and those exposed to polypharmacy (aOR = 1.29; p < 0.001) had higher odds of needing to have medicines deprescribed. The high rate of deprescribing potential warrants prompt action to increase patient safety and decrease polypharmacy. Pharmacist’s geriatric assessment and deprescribing-focused medication review could be used to lead a personalised approach.",
publisher = "Nature Research",
journal = "Scientific Reports",
title = "Deprescribing potential of commonly used medications among community-dwelling older adults: insights from a pharmacist’s geriatric assessment",
volume = "14",
number = "1",
pages = "6235",
doi = "10.1038/s41598-024-56780-1"
}

Bužančić, I., Držaić, M., Kummer, I., Ortner Hadžiabdić, M., Brkić, J.,& Fialová, D.. (2024). Deprescribing potential of commonly used medications among community-dwelling older adults: insights from a pharmacist’s geriatric assessment. in Scientific Reports
Nature Research., 14(1), 6235.
https://doi.org/10.1038/s41598-024-56780-1

Bužančić I, Držaić M, Kummer I, Ortner Hadžiabdić M, Brkić J, Fialová D. Deprescribing potential of commonly used medications among community-dwelling older adults: insights from a pharmacist’s geriatric assessment. in Scientific Reports. 2024;14(1):6235.
doi:10.1038/s41598-024-56780-1 .

Bužančić, Iva, Držaić, Margita, Kummer, Ingrid, Ortner Hadžiabdić, Maja, Brkić, Jovana, Fialová, Daniela, "Deprescribing potential of commonly used medications among community-dwelling older adults: insights from a pharmacist’s geriatric assessment" in Scientific Reports, 14, no. 1 (2024):6235,
https://doi.org/10.1038/s41598-024-56780-1 . .

TY  - JOUR
AU  - Petrović, Sanja
AU  - Kovačević, Milena
AU  - Vezmar-Kovačević, Sandra
AU  - Miljković, Branislava
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5597
AB  - Background: We aimed to characterize newer antiseizure medications (ASMs)-induced hepatotoxicity
in children and identify signals of disproportionate reporting of hepatotoxicity-related adverse drug
events (ADEs).
Research Design and Methods: Case reports reported to VigiBase were accessed using Empirica™
Signal software. To summarize characteristics of the retrieved cases, descriptive statistics were used.
A disproportionality analysis was conducted using the Multi-item Gamma Poisson Shrinker algorithm,
which calculates Empirical Bayesian Geometric Mean value and its lower and upper 95% confidence
limits (EB05 and EB95, respectively). EB05 > 2, N > 0 was considered a signal.
Results: Based on 870 analyzed cases, a higher proportion of cases was reported in girls than in boys
and in patients aged 2–11 years than in other age groups. Most cases were serious. In 25 cases,
hepatotoxicity resulted in death. A high proportion of patients (n = 275, 31.61%) experienced hyper-
sensitivity reactions, mostly due to lamotrigine. The disproportionality analysis yielded 17 signals
concerning felbamate, lamotrigine, levetiracetam, oxcarbazepine, stiripentol, and topiramate. Four
signals were for severe liver injury and concerned felbamate, lamotrigine, levetiracetam, and topira-
mate. Gender-biased reporting frequency was detected for four ASM-ADE combinations.
Conclusion: Our results should serve to raise clinicians’ awareness about the potential association
between several newer ASMs and drug-induced liver injury in children.
PB  - Taylor & Francis
T2  - Expert Opinion on Drug Metabolism & Toxicology
T1  - Hepatotoxicity of newer antiseizure medications in children: an overview and disproportionality analysis of VigiBase
VL  - 20
IS  - 3
SP  - 165
EP  - 173
DO  - 10.1080/17425255.2024.2322114
ER  - 

@article{
author = "Petrović, Sanja and Kovačević, Milena and Vezmar-Kovačević, Sandra and Miljković, Branislava",
year = "2024",
abstract = "Background: We aimed to characterize newer antiseizure medications (ASMs)-induced hepatotoxicity
in children and identify signals of disproportionate reporting of hepatotoxicity-related adverse drug
events (ADEs).
Research Design and Methods: Case reports reported to VigiBase were accessed using Empirica™
Signal software. To summarize characteristics of the retrieved cases, descriptive statistics were used.
A disproportionality analysis was conducted using the Multi-item Gamma Poisson Shrinker algorithm,
which calculates Empirical Bayesian Geometric Mean value and its lower and upper 95% confidence
limits (EB05 and EB95, respectively). EB05 > 2, N > 0 was considered a signal.
Results: Based on 870 analyzed cases, a higher proportion of cases was reported in girls than in boys
and in patients aged 2–11 years than in other age groups. Most cases were serious. In 25 cases,
hepatotoxicity resulted in death. A high proportion of patients (n = 275, 31.61%) experienced hyper-
sensitivity reactions, mostly due to lamotrigine. The disproportionality analysis yielded 17 signals
concerning felbamate, lamotrigine, levetiracetam, oxcarbazepine, stiripentol, and topiramate. Four
signals were for severe liver injury and concerned felbamate, lamotrigine, levetiracetam, and topira-
mate. Gender-biased reporting frequency was detected for four ASM-ADE combinations.
Conclusion: Our results should serve to raise clinicians’ awareness about the potential association
between several newer ASMs and drug-induced liver injury in children.",
publisher = "Taylor & Francis",
journal = "Expert Opinion on Drug Metabolism & Toxicology",
title = "Hepatotoxicity of newer antiseizure medications in children: an overview and disproportionality analysis of VigiBase",
volume = "20",
number = "3",
pages = "165-173",
doi = "10.1080/17425255.2024.2322114"
}

Petrović, S., Kovačević, M., Vezmar-Kovačević, S.,& Miljković, B.. (2024). Hepatotoxicity of newer antiseizure medications in children: an overview and disproportionality analysis of VigiBase. in Expert Opinion on Drug Metabolism & Toxicology
Taylor & Francis., 20(3), 165-173.
https://doi.org/10.1080/17425255.2024.2322114

Petrović S, Kovačević M, Vezmar-Kovačević S, Miljković B. Hepatotoxicity of newer antiseizure medications in children: an overview and disproportionality analysis of VigiBase. in Expert Opinion on Drug Metabolism & Toxicology. 2024;20(3):165-173.
doi:10.1080/17425255.2024.2322114 .

Petrović, Sanja, Kovačević, Milena, Vezmar-Kovačević, Sandra, Miljković, Branislava, "Hepatotoxicity of newer antiseizure medications in children: an overview and disproportionality analysis of VigiBase" in Expert Opinion on Drug Metabolism & Toxicology, 20, no. 3 (2024):165-173,
https://doi.org/10.1080/17425255.2024.2322114 . .

TY  - JOUR
AU  - Milosavljević, Filip
AU  - Molden, Espen
AU  - Ingelman-Sundberg, Magnus
AU  - Jukić, Marin
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5593
AB  - The aim of the study was to assess the clinical utility of currently available pharmacogenomic (PGx) tools compared with treatment as usual (TAU), using a meta-analysis of dichotomous and continuous antidepressant efficacy and tolerability data from previously published clinical trials. MEDLINE, clinicaltrial.gov, EU Clinical Trials Register, WHO ICTRP and CENTRAL were systematically searched; of the 962 results originally reviewed, 15 trials were included. Antidepressant efficacy was quantified by relative and absolute changes in symptom severity after eight weeks of treatment and by response and remission rates, while tolerability was estimated by the rate of study discontinuation for any reason. In the PGx-guided patients, symptom severity reduced by an average of 31.0% after eight weeks of treatment, compared to an average reduction of 26.8% in the TAU group. Accordingly, PGx-guided patients experienced a greater reduction in symptom severity of 3.4% (95%CI: 1.6-5.3%), which corresponded to a reduction in the Hamilton Depression score of 0.75 (0.30-1.21), a 37% (15-63%) higher remission rate, and an 18% (5-33%) higher response rate compared with TAU patients, while no difference was observed in discontinuation rate between groups. Notably, the majority of associations lost statistical significance when restricting the dataset to low risk of bias studies, while certain funnel plots suggested a potential publication bias favoring the reporting of statistically significant results. In summary, PGx tools marginally enhance antidepressant efficacy, but not antidepressant tolerability; thus, additional research and advancement of PGx tools are needed to improve integration of PGx in clinical pharmacotherapy of depression.
PB  - Elsevier B.V.
T2  - European Neuropsychopharmacology
T1  - Current level of evidence for improvement of antidepressant efficacy and
tolerability by pharmacogenomic-guided treatment: A Systematic review
and meta-analysis of randomized controlled clinical trials
VL  - 81
SP  - 43
EP  - 52
DO  - 10.1016/j.euroneuro.2024.01.005
ER  - 

@article{
author = "Milosavljević, Filip and Molden, Espen and Ingelman-Sundberg, Magnus and Jukić, Marin",
year = "2024",
abstract = "The aim of the study was to assess the clinical utility of currently available pharmacogenomic (PGx) tools compared with treatment as usual (TAU), using a meta-analysis of dichotomous and continuous antidepressant efficacy and tolerability data from previously published clinical trials. MEDLINE, clinicaltrial.gov, EU Clinical Trials Register, WHO ICTRP and CENTRAL were systematically searched; of the 962 results originally reviewed, 15 trials were included. Antidepressant efficacy was quantified by relative and absolute changes in symptom severity after eight weeks of treatment and by response and remission rates, while tolerability was estimated by the rate of study discontinuation for any reason. In the PGx-guided patients, symptom severity reduced by an average of 31.0% after eight weeks of treatment, compared to an average reduction of 26.8% in the TAU group. Accordingly, PGx-guided patients experienced a greater reduction in symptom severity of 3.4% (95%CI: 1.6-5.3%), which corresponded to a reduction in the Hamilton Depression score of 0.75 (0.30-1.21), a 37% (15-63%) higher remission rate, and an 18% (5-33%) higher response rate compared with TAU patients, while no difference was observed in discontinuation rate between groups. Notably, the majority of associations lost statistical significance when restricting the dataset to low risk of bias studies, while certain funnel plots suggested a potential publication bias favoring the reporting of statistically significant results. In summary, PGx tools marginally enhance antidepressant efficacy, but not antidepressant tolerability; thus, additional research and advancement of PGx tools are needed to improve integration of PGx in clinical pharmacotherapy of depression.",
publisher = "Elsevier B.V.",
journal = "European Neuropsychopharmacology",
title = "Current level of evidence for improvement of antidepressant efficacy and
tolerability by pharmacogenomic-guided treatment: A Systematic review
and meta-analysis of randomized controlled clinical trials",
volume = "81",
pages = "43-52",
doi = "10.1016/j.euroneuro.2024.01.005"
}

Milosavljević, F., Molden, E., Ingelman-Sundberg, M.,& Jukić, M.. (2024). Current level of evidence for improvement of antidepressant efficacy and
tolerability by pharmacogenomic-guided treatment: A Systematic review
and meta-analysis of randomized controlled clinical trials. in European Neuropsychopharmacology
Elsevier B.V.., 81, 43-52.
https://doi.org/10.1016/j.euroneuro.2024.01.005

Milosavljević F, Molden E, Ingelman-Sundberg M, Jukić M. Current level of evidence for improvement of antidepressant efficacy and
tolerability by pharmacogenomic-guided treatment: A Systematic review
and meta-analysis of randomized controlled clinical trials. in European Neuropsychopharmacology. 2024;81:43-52.
doi:10.1016/j.euroneuro.2024.01.005 .

Milosavljević, Filip, Molden, Espen, Ingelman-Sundberg, Magnus, Jukić, Marin, "Current level of evidence for improvement of antidepressant efficacy and
tolerability by pharmacogenomic-guided treatment: A Systematic review
and meta-analysis of randomized controlled clinical trials" in European Neuropsychopharmacology, 81 (2024):43-52,
https://doi.org/10.1016/j.euroneuro.2024.01.005 . .

TY  - JOUR
AU  - Božić, Dragana
AU  - Bufan, Biljana
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5603
AB  - Antibacterial vaccines play a central role in modern medicine by providing an effective approach to
combating infectious diseases caused by bacteria. The importance of these vaccines lies in their ability to
stimulate the immune system to recognise and neutralise bacterial pathogens, or exotoxins produced by them,
thereby preventing, or mitigating the severity of bacterial infections. The development and widespread use of
antibacterial vaccines have contributed significantly to reducing the global burden of diseases such as pneumonia,
meningitis, and sepsis.
Today, the global increase in vaccine-preventable diseases is a worrying trend that is closely linked to the
emergence and advocacy of anti-vaccination policies. According to the latest World Health Organisation report,
vaccination coverage in Serbia has declined over the past decade, jeopardising the collective immunity and led to
recent outbreaks of vaccine-preventable diseases such as whooping cough and measles. Understanding the
significance of antibacterial vaccines underscores their importance in promoting individual and community immunity,
which ultimately leads to a healthier population and the prevention of antibiotic resistance.
This paper summarises the main characteristics of the different types of antibacterial vaccines, such as whole cell
vaccines, subunit vaccines and toxoid vaccines, and provides an overview of the types of bacterial antigens
contained in vaccines available for mandatory immunisation (vaccines against tuberculosis, diphtheria, tetanus,
pertussis, Haemophilus influenzae and pneumoccus) or for non-mandatory immunisation (meningococcal vaccine,
typhoid vaccine, cholera vaccine).
AB  - Antibakterijske vakcine igraju glavnu ulogu u savremenoj medicini obezbeđujući efikasan pristup u borbi protiv
zaraznih bolesti izazvanih bakterijama. Važnost ovih vakcina leži u njihovoj sposobnosti da stimulišu imunski sistem
da prepozna i neutrališe bakterijske patogene, ili egzotoksine koje oni proizvode, čime sprečavaju ili ublažavaju
ozbiljnost bakterijskih infekcija. Razvoj i široka upotreba antibakterijskih vakcina značajno su doprineli smanjenju
globalnog tereta bolesti kao što su pneumonija, meningitis i sepsa.
Danas je globalni porast bolesti koje se mogu sprečiti vakcinama zabrinjavajući trend koji je usko povezan sa
pojavom i zagovaranjem politike protiv vakcinacije. Prema poslednjem izveštaju Svetske zdravstvene organizacije,
pokrivenost vakcinacijom u Srbiji je opala tokom protekle decenije, što je ugrozilo kolektivni imunitet i dovelo do
nedavnih izbijanja bolesti koje se mogu sprečiti vakcinom, poput velikog kašlja i malih boginja. Razumevanje
značaja antibakterijskih vakcina naglašava njihov značaj u promovisanju imuniteta pojedinca i zajednice, što na
kraju dovodi do zdravije populacije i prevencije rezistencije na antibiotike.
Ovaj rad sumira glavne karakteristike različitih tipova antibakterijskih vakcina, kao što su celo ćelijske vakcine,
podjedinične vakcine i toksoidne vakcine, i daje pregled tipova bakterijskih antigena sadržanih u vakcinama
dostupnim za obaveznu imunizaciju (vakcine protiv tuberkuloze, difterije, tetanusa, pertusisa, Haemophilus
influenzae i pneumoka) ili za neobaveznu imunizaciju (vakcina protiv meningokoka, tifusa i kolere).
PB  - Udruženja za preventivnu pedijatriju Srbije
T2  - Preventive Paediatrics Journal of the Association for Preventive Pediatrics of Serbia / Preventivna pedijatrija Časopis Udruženja za preventivnu pedijatriju Srbije
T1  - An overview of current vacccines for the prophylaxis of bacterical infections Pres
T1  - Pregled aktuelnih vakcina za profilaksu bakterijskih infekcija
VL  - 10
IS  - 1-2
SP  - 19
EP  - 26
DO  - 10.46793/PP240214004B
DO  - 10.46793/PP240214004B
ER  - 

@article{
author = "Božić, Dragana and Bufan, Biljana",
year = "2024",
abstract = "Antibacterial vaccines play a central role in modern medicine by providing an effective approach to
combating infectious diseases caused by bacteria. The importance of these vaccines lies in their ability to
stimulate the immune system to recognise and neutralise bacterial pathogens, or exotoxins produced by them,
thereby preventing, or mitigating the severity of bacterial infections. The development and widespread use of
antibacterial vaccines have contributed significantly to reducing the global burden of diseases such as pneumonia,
meningitis, and sepsis.
Today, the global increase in vaccine-preventable diseases is a worrying trend that is closely linked to the
emergence and advocacy of anti-vaccination policies. According to the latest World Health Organisation report,
vaccination coverage in Serbia has declined over the past decade, jeopardising the collective immunity and led to
recent outbreaks of vaccine-preventable diseases such as whooping cough and measles. Understanding the
significance of antibacterial vaccines underscores their importance in promoting individual and community immunity,
which ultimately leads to a healthier population and the prevention of antibiotic resistance.
This paper summarises the main characteristics of the different types of antibacterial vaccines, such as whole cell
vaccines, subunit vaccines and toxoid vaccines, and provides an overview of the types of bacterial antigens
contained in vaccines available for mandatory immunisation (vaccines against tuberculosis, diphtheria, tetanus,
pertussis, Haemophilus influenzae and pneumoccus) or for non-mandatory immunisation (meningococcal vaccine,
typhoid vaccine, cholera vaccine)., Antibakterijske vakcine igraju glavnu ulogu u savremenoj medicini obezbeđujući efikasan pristup u borbi protiv
zaraznih bolesti izazvanih bakterijama. Važnost ovih vakcina leži u njihovoj sposobnosti da stimulišu imunski sistem
da prepozna i neutrališe bakterijske patogene, ili egzotoksine koje oni proizvode, čime sprečavaju ili ublažavaju
ozbiljnost bakterijskih infekcija. Razvoj i široka upotreba antibakterijskih vakcina značajno su doprineli smanjenju
globalnog tereta bolesti kao što su pneumonija, meningitis i sepsa.
Danas je globalni porast bolesti koje se mogu sprečiti vakcinama zabrinjavajući trend koji je usko povezan sa
pojavom i zagovaranjem politike protiv vakcinacije. Prema poslednjem izveštaju Svetske zdravstvene organizacije,
pokrivenost vakcinacijom u Srbiji je opala tokom protekle decenije, što je ugrozilo kolektivni imunitet i dovelo do
nedavnih izbijanja bolesti koje se mogu sprečiti vakcinom, poput velikog kašlja i malih boginja. Razumevanje
značaja antibakterijskih vakcina naglašava njihov značaj u promovisanju imuniteta pojedinca i zajednice, što na
kraju dovodi do zdravije populacije i prevencije rezistencije na antibiotike.
Ovaj rad sumira glavne karakteristike različitih tipova antibakterijskih vakcina, kao što su celo ćelijske vakcine,
podjedinične vakcine i toksoidne vakcine, i daje pregled tipova bakterijskih antigena sadržanih u vakcinama
dostupnim za obaveznu imunizaciju (vakcine protiv tuberkuloze, difterije, tetanusa, pertusisa, Haemophilus
influenzae i pneumoka) ili za neobaveznu imunizaciju (vakcina protiv meningokoka, tifusa i kolere).",
publisher = "Udruženja za preventivnu pedijatriju Srbije",
journal = "Preventive Paediatrics Journal of the Association for Preventive Pediatrics of Serbia / Preventivna pedijatrija Časopis Udruženja za preventivnu pedijatriju Srbije",
title = "An overview of current vacccines for the prophylaxis of bacterical infections Pres, Pregled aktuelnih vakcina za profilaksu bakterijskih infekcija",
volume = "10",
number = "1-2",
pages = "19-26",
doi = "10.46793/PP240214004B, 10.46793/PP240214004B"
}

Božić, D.,& Bufan, B.. (2024). An overview of current vacccines for the prophylaxis of bacterical infections Pres. in Preventive Paediatrics Journal of the Association for Preventive Pediatrics of Serbia / Preventivna pedijatrija Časopis Udruženja za preventivnu pedijatriju Srbije
Udruženja za preventivnu pedijatriju Srbije., 10(1-2), 19-26.
https://doi.org/10.46793/PP240214004B

Božić D, Bufan B. An overview of current vacccines for the prophylaxis of bacterical infections Pres. in Preventive Paediatrics Journal of the Association for Preventive Pediatrics of Serbia / Preventivna pedijatrija Časopis Udruženja za preventivnu pedijatriju Srbije. 2024;10(1-2):19-26.
doi:10.46793/PP240214004B .

Božić, Dragana, Bufan, Biljana, "An overview of current vacccines for the prophylaxis of bacterical infections Pres" in Preventive Paediatrics Journal of the Association for Preventive Pediatrics of Serbia / Preventivna pedijatrija Časopis Udruženja za preventivnu pedijatriju Srbije, 10, no. 1-2 (2024):19-26,
https://doi.org/10.46793/PP240214004B . .

TY  - JOUR
AU  - Živančević, Katarina
AU  - Baralić, Katarina
AU  - Vukelić, Dragana
AU  - Marić, Đurđica
AU  - Kotur-Stevuljević, Jelena
AU  - Ivanišević, Jasmina
AU  - Savić, Miroslav
AU  - Batinić, Bojan
AU  - Janković, Radmila
AU  - Buha-Đorđević, Aleksandra
AU  - Antonijević-Miljaković, Evica
AU  - Ćurčić, Marijana
AU  - Bulat, Zorica
AU  - Antonijević, Biljana
AU  - Đukić-Ćosić, Danijela
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5614
AB  - Metals exert detrimental effects on various systems within the body, including the nervous system. Nevertheless, the dose-response relationship concerning the administration of low doses of metal mixtures remains inadequately explored. The assessment of neurotoxic effects of lead, cadmium, mercury, and arsenic mixture (MIX) administered at low dose ranges, was conducted using an in vivo approach. A subacute study was conducted on a rat model consisting of a control and five treatment groups subjected to oral exposure with gradually increasing doses (from MIX 1 to MIX 5). The results indicated that behavioural patterns in an already developed nervous system displayed a reduced susceptibility to the metal mixture exposure with tendency of higher doses to alter short term memory. However, the vulnerability of the mature brain to even minimal amounts of the investigated metal mixture was evident, particularly in the context of oxidative stress. Moreover, the study highlights superoxide dismutase's sensitivity as an early-stage neurotoxicity marker, as indicated by dose-dependent induction of oxidative stress in the brain revealed through Benchmark analysis. The narrowest Benchmark Dose Interval (BMDI) for superoxide dismutase (SOD) activity (1e-06 - 3.18e-05 mg As/kg b.w./day) indicates that arsenic may dictate the alterations in SOD activity when co-exposed with the other examined metals. The predicted Benchmark doses for oxidative stress parameters were very low, supporting “no-threshold” concept. Histopathological alterations were most severe in the groups treated with higher doses of metal mixture. Similarly, the brain acetylcholinesterase (AChE) activity demonstrated a dose-dependent decrease significant in higher doses, while BMDI suggested Cd as the main contributor in the examined metal mixture. These findings imply varying susceptibility of neurotoxic endpoints to different doses of environmentally relevant metal mixtures, advocating for risk assessment and regulatory measures to address metal pollution and enhance remediation strategies.
PB  - Elsevier Inc.
T2  - Environmental Research
T1  - Neurotoxic effects of low dose ranges of environmental metal mixture in a rat model: The benchmark approach
VL  - 252
IS  - 1
DO  - 10.1016/j.envres.2024.118680
ER  - 

@article{
author = "Živančević, Katarina and Baralić, Katarina and Vukelić, Dragana and Marić, Đurđica and Kotur-Stevuljević, Jelena and Ivanišević, Jasmina and Savić, Miroslav and Batinić, Bojan and Janković, Radmila and Buha-Đorđević, Aleksandra and Antonijević-Miljaković, Evica and Ćurčić, Marijana and Bulat, Zorica and Antonijević, Biljana and Đukić-Ćosić, Danijela",
year = "2024",
abstract = "Metals exert detrimental effects on various systems within the body, including the nervous system. Nevertheless, the dose-response relationship concerning the administration of low doses of metal mixtures remains inadequately explored. The assessment of neurotoxic effects of lead, cadmium, mercury, and arsenic mixture (MIX) administered at low dose ranges, was conducted using an in vivo approach. A subacute study was conducted on a rat model consisting of a control and five treatment groups subjected to oral exposure with gradually increasing doses (from MIX 1 to MIX 5). The results indicated that behavioural patterns in an already developed nervous system displayed a reduced susceptibility to the metal mixture exposure with tendency of higher doses to alter short term memory. However, the vulnerability of the mature brain to even minimal amounts of the investigated metal mixture was evident, particularly in the context of oxidative stress. Moreover, the study highlights superoxide dismutase's sensitivity as an early-stage neurotoxicity marker, as indicated by dose-dependent induction of oxidative stress in the brain revealed through Benchmark analysis. The narrowest Benchmark Dose Interval (BMDI) for superoxide dismutase (SOD) activity (1e-06 - 3.18e-05 mg As/kg b.w./day) indicates that arsenic may dictate the alterations in SOD activity when co-exposed with the other examined metals. The predicted Benchmark doses for oxidative stress parameters were very low, supporting “no-threshold” concept. Histopathological alterations were most severe in the groups treated with higher doses of metal mixture. Similarly, the brain acetylcholinesterase (AChE) activity demonstrated a dose-dependent decrease significant in higher doses, while BMDI suggested Cd as the main contributor in the examined metal mixture. These findings imply varying susceptibility of neurotoxic endpoints to different doses of environmentally relevant metal mixtures, advocating for risk assessment and regulatory measures to address metal pollution and enhance remediation strategies.",
publisher = "Elsevier Inc.",
journal = "Environmental Research",
title = "Neurotoxic effects of low dose ranges of environmental metal mixture in a rat model: The benchmark approach",
volume = "252",
number = "1",
doi = "10.1016/j.envres.2024.118680"
}

Živančević, K., Baralić, K., Vukelić, D., Marić, Đ., Kotur-Stevuljević, J., Ivanišević, J., Savić, M., Batinić, B., Janković, R., Buha-Đorđević, A., Antonijević-Miljaković, E., Ćurčić, M., Bulat, Z., Antonijević, B.,& Đukić-Ćosić, D.. (2024). Neurotoxic effects of low dose ranges of environmental metal mixture in a rat model: The benchmark approach. in Environmental Research
Elsevier Inc.., 252(1).
https://doi.org/10.1016/j.envres.2024.118680

Živančević K, Baralić K, Vukelić D, Marić Đ, Kotur-Stevuljević J, Ivanišević J, Savić M, Batinić B, Janković R, Buha-Đorđević A, Antonijević-Miljaković E, Ćurčić M, Bulat Z, Antonijević B, Đukić-Ćosić D. Neurotoxic effects of low dose ranges of environmental metal mixture in a rat model: The benchmark approach. in Environmental Research. 2024;252(1).
doi:10.1016/j.envres.2024.118680 .

Živančević, Katarina, Baralić, Katarina, Vukelić, Dragana, Marić, Đurđica, Kotur-Stevuljević, Jelena, Ivanišević, Jasmina, Savić, Miroslav, Batinić, Bojan, Janković, Radmila, Buha-Đorđević, Aleksandra, Antonijević-Miljaković, Evica, Ćurčić, Marijana, Bulat, Zorica, Antonijević, Biljana, Đukić-Ćosić, Danijela, "Neurotoxic effects of low dose ranges of environmental metal mixture in a rat model: The benchmark approach" in Environmental Research, 252, no. 1 (2024),
https://doi.org/10.1016/j.envres.2024.118680 . .

TY  - JOUR
AU  - Varga, Filip
AU  - Liber, Zlatko
AU  - Turudić, Ante
AU  - Jakše, Jernej
AU  - Juzbašić, Lea
AU  - Jeran, Nina
AU  - Grdiša, Martina
AU  - Zbiljić, Miloš
AU  - Šatović, Zlatko
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5609
AB  - Teucrium montanum L. and T. capitatum L. are two plant species with overlapping distribution
in the Balkan Peninsula, especially in Croatia, where several occurrences of their putative hybrid
species T. × rohlenae have been recorded. Next-generation sequencing of both species and de novo
assembly was carried out resulting in 120 contigs for T. montanum and 1685 contigs for T. capitatum
assembled. The Dig-up primers pipeline was used for SSR mining of both assemblies, applying
different criteria that resulted in 112 SSR candidates for testing. A subset of 41 SSRs was selected and
after two rounds of testing, twelve SSRs were developed and characterized. A total of 232 alleles
were detected with 5 to 29 alleles per locus, based on the test sample. The genetic diversity analysis
of three Teucrium taxa from a single location revealed a higher level of diversity in T. montanum
than in T. capitatum with intermediate values for the hybrid species. The NeighborNet diagram and
genetic structure analysis grouped the taxa into two separate clusters, the first of which consisted
exclusively of T. montanum, while the other was composed of intermixed T. capitatum and T. × rohlenae
individuals. The availability of SSR markers for two Teucrium taxa will allow in-depth analysis
of genetic diversity and structure, as well as molecular identification of their putative hybrids in
the future.
PB  - MDPI
T2  - Diversity
T1  - Joint Identification and Application of Microsatellite Markers in Genetic Diversity Study of Closely Related Species Teucrium montanum, T. capitatum and Their Natural Hybrid
VL  - 16
IS  - 4
SP  - 204
DO  - 10.3390/d16040206
ER  - 

@article{
author = "Varga, Filip and Liber, Zlatko and Turudić, Ante and Jakše, Jernej and Juzbašić, Lea and Jeran, Nina and Grdiša, Martina and Zbiljić, Miloš and Šatović, Zlatko",
year = "2024",
abstract = "Teucrium montanum L. and T. capitatum L. are two plant species with overlapping distribution
in the Balkan Peninsula, especially in Croatia, where several occurrences of their putative hybrid
species T. × rohlenae have been recorded. Next-generation sequencing of both species and de novo
assembly was carried out resulting in 120 contigs for T. montanum and 1685 contigs for T. capitatum
assembled. The Dig-up primers pipeline was used for SSR mining of both assemblies, applying
different criteria that resulted in 112 SSR candidates for testing. A subset of 41 SSRs was selected and
after two rounds of testing, twelve SSRs were developed and characterized. A total of 232 alleles
were detected with 5 to 29 alleles per locus, based on the test sample. The genetic diversity analysis
of three Teucrium taxa from a single location revealed a higher level of diversity in T. montanum
than in T. capitatum with intermediate values for the hybrid species. The NeighborNet diagram and
genetic structure analysis grouped the taxa into two separate clusters, the first of which consisted
exclusively of T. montanum, while the other was composed of intermixed T. capitatum and T. × rohlenae
individuals. The availability of SSR markers for two Teucrium taxa will allow in-depth analysis
of genetic diversity and structure, as well as molecular identification of their putative hybrids in
the future.",
publisher = "MDPI",
journal = "Diversity",
title = "Joint Identification and Application of Microsatellite Markers in Genetic Diversity Study of Closely Related Species Teucrium montanum, T. capitatum and Their Natural Hybrid",
volume = "16",
number = "4",
pages = "204",
doi = "10.3390/d16040206"
}

Varga, F., Liber, Z., Turudić, A., Jakše, J., Juzbašić, L., Jeran, N., Grdiša, M., Zbiljić, M.,& Šatović, Z.. (2024). Joint Identification and Application of Microsatellite Markers in Genetic Diversity Study of Closely Related Species Teucrium montanum, T. capitatum and Their Natural Hybrid. in Diversity
MDPI., 16(4), 204.
https://doi.org/10.3390/d16040206

Varga F, Liber Z, Turudić A, Jakše J, Juzbašić L, Jeran N, Grdiša M, Zbiljić M, Šatović Z. Joint Identification and Application of Microsatellite Markers in Genetic Diversity Study of Closely Related Species Teucrium montanum, T. capitatum and Their Natural Hybrid. in Diversity. 2024;16(4):204.
doi:10.3390/d16040206 .

Varga, Filip, Liber, Zlatko, Turudić, Ante, Jakše, Jernej, Juzbašić, Lea, Jeran, Nina, Grdiša, Martina, Zbiljić, Miloš, Šatović, Zlatko, "Joint Identification and Application of Microsatellite Markers in Genetic Diversity Study of Closely Related Species Teucrium montanum, T. capitatum and Their Natural Hybrid" in Diversity, 16, no. 4 (2024):204,
https://doi.org/10.3390/d16040206 . .

TY  - JOUR
AU  - Kovačević, Milena
AU  - Ivanović, Nevena
AU  - Protić, Ana
AU  - Milenković, Danijela
AU  - Mandinić, Zoran
AU  - Puzović, Dragana
AU  - Bajčetić, Miloš
AU  - Popadić, Dušan
AU  - Parojčić, Jelena
AU  - Malenović, Anđelija
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5596
AB  - Digital transformation in education and relevant calls for action are recognised global priorities aimed to support education and training in the digital age. The experiences from the emergency remote teaching during the COVID-19 pandemic and recent research findings reveal relevant advantages, challenges, as well as different students and teaching staff perspectives which should be carefully considered and integrated into institutional strategies and policies to improve and enrich students’ learning experience. The present study aimed to explore health sciences students' perspectives on online teaching and learning (T&L) in four domains—Satisfaction, Motivation, Interaction and Challenges, and to identify the preferred T&L models or tools. A total of 1,041 responses were collected, including 476 dental medicine (45.7%), 399 pharmacy (38.3%), and 166 medical students (15.9%). Overall Satisfaction was quite high (4.0 ± 1.2; out of 5), followed by satisfactory Interaction (3.4 ± 1.4), whereas Motivation was quite low on average (3.0 ± 1.5). Challenges in online T&L were not markedly expressed (3.0 ± 1.6). The majority of students (46.6%) opted for the blended model as the preferred T&L model. Moodle lessons were perceived as the most useful online T&L tool (45.5% of students), followed by video materials (32.2%). Other T&L tools which require more active student's role, such as short test/quiz, and group or team assignment were declared as less favourable. The findings from the current study may contribute to raising the awareness of academic staff and higher institutions management about the student attitudes and needs related to online T&L and inform institutional planning, decision-making and policy development.
PB  - John Wiley & Sons Ltd
T2  - European Journal of Education
T1  - Health sciences students' perspectives on online teaching and learning: Extending the implications beyond the COVID-19 pandemic
DO  - 10.1111/ejed.12660
ER  - 

@article{
author = "Kovačević, Milena and Ivanović, Nevena and Protić, Ana and Milenković, Danijela and Mandinić, Zoran and Puzović, Dragana and Bajčetić, Miloš and Popadić, Dušan and Parojčić, Jelena and Malenović, Anđelija",
year = "2024",
abstract = "Digital transformation in education and relevant calls for action are recognised global priorities aimed to support education and training in the digital age. The experiences from the emergency remote teaching during the COVID-19 pandemic and recent research findings reveal relevant advantages, challenges, as well as different students and teaching staff perspectives which should be carefully considered and integrated into institutional strategies and policies to improve and enrich students’ learning experience. The present study aimed to explore health sciences students' perspectives on online teaching and learning (T&L) in four domains—Satisfaction, Motivation, Interaction and Challenges, and to identify the preferred T&L models or tools. A total of 1,041 responses were collected, including 476 dental medicine (45.7%), 399 pharmacy (38.3%), and 166 medical students (15.9%). Overall Satisfaction was quite high (4.0 ± 1.2; out of 5), followed by satisfactory Interaction (3.4 ± 1.4), whereas Motivation was quite low on average (3.0 ± 1.5). Challenges in online T&L were not markedly expressed (3.0 ± 1.6). The majority of students (46.6%) opted for the blended model as the preferred T&L model. Moodle lessons were perceived as the most useful online T&L tool (45.5% of students), followed by video materials (32.2%). Other T&L tools which require more active student's role, such as short test/quiz, and group or team assignment were declared as less favourable. The findings from the current study may contribute to raising the awareness of academic staff and higher institutions management about the student attitudes and needs related to online T&L and inform institutional planning, decision-making and policy development.",
publisher = "John Wiley & Sons Ltd",
journal = "European Journal of Education",
title = "Health sciences students' perspectives on online teaching and learning: Extending the implications beyond the COVID-19 pandemic",
doi = "10.1111/ejed.12660"
}

Kovačević, M., Ivanović, N., Protić, A., Milenković, D., Mandinić, Z., Puzović, D., Bajčetić, M., Popadić, D., Parojčić, J.,& Malenović, A.. (2024). Health sciences students' perspectives on online teaching and learning: Extending the implications beyond the COVID-19 pandemic. in European Journal of Education
John Wiley & Sons Ltd..
https://doi.org/10.1111/ejed.12660

Kovačević M, Ivanović N, Protić A, Milenković D, Mandinić Z, Puzović D, Bajčetić M, Popadić D, Parojčić J, Malenović A. Health sciences students' perspectives on online teaching and learning: Extending the implications beyond the COVID-19 pandemic. in European Journal of Education. 2024;.
doi:10.1111/ejed.12660 .

Kovačević, Milena, Ivanović, Nevena, Protić, Ana, Milenković, Danijela, Mandinić, Zoran, Puzović, Dragana, Bajčetić, Miloš, Popadić, Dušan, Parojčić, Jelena, Malenović, Anđelija, "Health sciences students' perspectives on online teaching and learning: Extending the implications beyond the COVID-19 pandemic" in European Journal of Education (2024),
https://doi.org/10.1111/ejed.12660 . .

TY  - JOUR
AU  - Popović-Nikolić, Marija
AU  - Čakar, Mira
AU  - Todorović, Nina
AU  - Nikolić, Katarina
AU  - Popović, Gordana
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5589
AB  - The acid–base equilibria of raloxifene and the mutual influence of pH and solubility enhancers on the solubility of raloxifene
hydrochloride were studied. The apparent ionization constants (pKa*) of raloxifene were determined potentiometrically in
methanol–water mixtures (45%-55% w/w), and the pKa values (pKa1 = 7.21 ± 0.02, pKa2 = 7.86 ± 0.02, pKa3 = 9.97 ± 0.04)
defining ionization in aqueous media were obtained by extrapolating the pKa* values to 0% of methanol. The obtained pKa
values were assigned to the corresponding ionization centers. Based on the ionization constants obtained in this study, the
distribution of the equilibrium forms of raloxifene was calculated. The solubility of raloxifene hydrochloride in 0.01 M
HCl, acetate buffer pH 4.5 and phosphate buffer pH 6.8 was studied with and without the presence of β-CD, HP-β-CD and
polysorbate 80. The most effective solubility enhancer of raloxifene hydrochloride in 0.01 M HCl was polysorbate 80 at a
concentration of 0.5%, while the influence of this enhancer in phosphate buffer pH 6.8 was negligible. The highest solubility
of raloxifene hydrochloride in acetate and phosphate buffer was achieved in the presence of 10–
3 M HP–β-CD which
was 1.3-fold higher in both 0.01 M HCl and acetate buffer and 2.3-fold higher in phosphate buffer than in the presence of
10–
3 M β-CD.
PB  - Springer Nature
T2  - Chemical Papers
T1  - Study on protolytic equilibria and the effects of pH, cyclodextrins and polysorbate 80 on solubility of raloxifene hydrochloride
DO  - 10.1007/s11696-024-03399-1
ER  - 

@article{
author = "Popović-Nikolić, Marija and Čakar, Mira and Todorović, Nina and Nikolić, Katarina and Popović, Gordana",
year = "2024",
abstract = "The acid–base equilibria of raloxifene and the mutual influence of pH and solubility enhancers on the solubility of raloxifene
hydrochloride were studied. The apparent ionization constants (pKa*) of raloxifene were determined potentiometrically in
methanol–water mixtures (45%-55% w/w), and the pKa values (pKa1 = 7.21 ± 0.02, pKa2 = 7.86 ± 0.02, pKa3 = 9.97 ± 0.04)
defining ionization in aqueous media were obtained by extrapolating the pKa* values to 0% of methanol. The obtained pKa
values were assigned to the corresponding ionization centers. Based on the ionization constants obtained in this study, the
distribution of the equilibrium forms of raloxifene was calculated. The solubility of raloxifene hydrochloride in 0.01 M
HCl, acetate buffer pH 4.5 and phosphate buffer pH 6.8 was studied with and without the presence of β-CD, HP-β-CD and
polysorbate 80. The most effective solubility enhancer of raloxifene hydrochloride in 0.01 M HCl was polysorbate 80 at a
concentration of 0.5%, while the influence of this enhancer in phosphate buffer pH 6.8 was negligible. The highest solubility
of raloxifene hydrochloride in acetate and phosphate buffer was achieved in the presence of 10–
3 M HP–β-CD which
was 1.3-fold higher in both 0.01 M HCl and acetate buffer and 2.3-fold higher in phosphate buffer than in the presence of
10–
3 M β-CD.",
publisher = "Springer Nature",
journal = "Chemical Papers",
title = "Study on protolytic equilibria and the effects of pH, cyclodextrins and polysorbate 80 on solubility of raloxifene hydrochloride",
doi = "10.1007/s11696-024-03399-1"
}

Popović-Nikolić, M., Čakar, M., Todorović, N., Nikolić, K.,& Popović, G.. (2024). Study on protolytic equilibria and the effects of pH, cyclodextrins and polysorbate 80 on solubility of raloxifene hydrochloride. in Chemical Papers
Springer Nature..
https://doi.org/10.1007/s11696-024-03399-1

Popović-Nikolić M, Čakar M, Todorović N, Nikolić K, Popović G. Study on protolytic equilibria and the effects of pH, cyclodextrins and polysorbate 80 on solubility of raloxifene hydrochloride. in Chemical Papers. 2024;.
doi:10.1007/s11696-024-03399-1 .

Popović-Nikolić, Marija, Čakar, Mira, Todorović, Nina, Nikolić, Katarina, Popović, Gordana, "Study on protolytic equilibria and the effects of pH, cyclodextrins and polysorbate 80 on solubility of raloxifene hydrochloride" in Chemical Papers (2024),
https://doi.org/10.1007/s11696-024-03399-1 . .