All Publications

Link to this page

Selectivity of N(2)-substituted oxotriazinoindole aldose reductase inhibitors is determined by the interaction pattern with Pro301-Arg312 loop of aldehyde reductase

Kováčiková, Lucia; Gaikwad, Sunil; Almášiová, Kristína; Almássy, Ambroz; Addová, Gabriela; Májeková, Magdaléna; Hanquet, Gilles; Dobričić, Vladimir; Boháč, Andrej; Štefek, Milan

TY  - JOUR
AU  - Kováčiková, Lucia
AU  - Gaikwad, Sunil
AU  - Almášiová, Kristína
AU  - Almássy, Ambroz
AU  - Addová, Gabriela
AU  - Májeková, Magdaléna
AU  - Hanquet, Gilles
AU  - Dobričić, Vladimir
AU  - Boháč, Andrej
AU  - Štefek, Milan
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5545
AB  - Novel oxotriazinoindoles (OTIs) were recently reported as highly efficient and selective aldose reductase inhibitors. Here, a series of novel N(2)-substituted oxotriazinoindoles was developed with the aim to investigate molecular interactions within the aldose reductase (ALR2) inhibitor binding site. About twice increased inhibition efficacy of the most efficient derivative 14 (N(2)-CH2CH2COOH) compared to the unsubstituted lead OTI was obtained, yet at the expense of selectivity relative to anti-target aldehyde reductase (ALR1). To explain the major drop in selectivity, observed also in other N(2)-substituted derivatives, in silico molecular modeling approach revealed the role of extra interactions with the residues of Arg309, Arg312 and Met302 located in the additional C-terminal loop of ALR1 missing in ALR2, which can prevent or enhance binding in ALR1. These key findings will be used for development of the next generation of selective OTI inhibitors. (Figure presented.).
PB  - Springer
T2  - Medicinal Chemistry Research
T1  - Selectivity of N(2)-substituted oxotriazinoindole aldose reductase inhibitors is determined by the interaction pattern with Pro301-Arg312 loop of aldehyde reductase
DO  - 10.1007/s00044-024-03194-3
ER  - 
@article{
author = "Kováčiková, Lucia and Gaikwad, Sunil and Almášiová, Kristína and Almássy, Ambroz and Addová, Gabriela and Májeková, Magdaléna and Hanquet, Gilles and Dobričić, Vladimir and Boháč, Andrej and Štefek, Milan",
abstract = "Novel oxotriazinoindoles (OTIs) were recently reported as highly efficient and selective aldose reductase inhibitors. Here, a series of novel N(2)-substituted oxotriazinoindoles was developed with the aim to investigate molecular interactions within the aldose reductase (ALR2) inhibitor binding site. About twice increased inhibition efficacy of the most efficient derivative 14 (N(2)-CH2CH2COOH) compared to the unsubstituted lead OTI was obtained, yet at the expense of selectivity relative to anti-target aldehyde reductase (ALR1). To explain the major drop in selectivity, observed also in other N(2)-substituted derivatives, in silico molecular modeling approach revealed the role of extra interactions with the residues of Arg309, Arg312 and Met302 located in the additional C-terminal loop of ALR1 missing in ALR2, which can prevent or enhance binding in ALR1. These key findings will be used for development of the next generation of selective OTI inhibitors. (Figure presented.).",
publisher = "Springer",
journal = "Medicinal Chemistry Research",
title = "Selectivity of N(2)-substituted oxotriazinoindole aldose reductase inhibitors is determined by the interaction pattern with Pro301-Arg312 loop of aldehyde reductase",
doi = "10.1007/s00044-024-03194-3"
}
Kováčiková, L., Gaikwad, S., Almášiová, K., Almássy, A., Addová, G., Májeková, M., Hanquet, G., Dobričić, V., Boháč, A.,& Štefek, M..Selectivity of N(2)-substituted oxotriazinoindole aldose reductase inhibitors is determined by the interaction pattern with Pro301-Arg312 loop of aldehyde reductase. in Medicinal Chemistry Research
Springer..
https://doi.org/10.1007/s00044-024-03194-3
Kováčiková L, Gaikwad S, Almášiová K, Almássy A, Addová G, Májeková M, Hanquet G, Dobričić V, Boháč A, Štefek M. Selectivity of N(2)-substituted oxotriazinoindole aldose reductase inhibitors is determined by the interaction pattern with Pro301-Arg312 loop of aldehyde reductase. in Medicinal Chemistry Research..
doi:10.1007/s00044-024-03194-3 .
Kováčiková, Lucia, Gaikwad, Sunil, Almášiová, Kristína, Almássy, Ambroz, Addová, Gabriela, Májeková, Magdaléna, Hanquet, Gilles, Dobričić, Vladimir, Boháč, Andrej, Štefek, Milan, "Selectivity of N(2)-substituted oxotriazinoindole aldose reductase inhibitors is determined by the interaction pattern with Pro301-Arg312 loop of aldehyde reductase" in Medicinal Chemistry Research,
https://doi.org/10.1007/s00044-024-03194-3 . .

Treatment of vascular non-iatrogenic injuries of upper and lower extremities in tertiary vascular center

Davidović, Lazar B.; Končar, Igor B.; Dragaš, Marko V.; Marković, Miroslav D.; Bogavac-Stanojević, Nataša; Vujčić, Aleksandra D.; Mitrović, Aleksandar C.; Ilić, Nikola S.; Trailović, R.D.; Kostić, Dušan M.

TY  - JOUR
AU  - Davidović, Lazar B.
AU  - Končar, Igor B.
AU  - Dragaš, Marko V.
AU  - Marković, Miroslav D.
AU  - Bogavac-Stanojević, Nataša
AU  - Vujčić, Aleksandra D.
AU  - Mitrović, Aleksandar C.
AU  - Ilić, Nikola S.
AU  - Trailović, R.D.
AU  - Kostić, Dušan M.
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4430
AB  - BACKGROUND: The purpose of this study was to examine demographic and baseline characteristics of patients with vascular injuries of extremities and to define the most relevant factors which influenced an early outcome, as well as limb salvage after the management of vascular trauma. METHODS: This study used the database that included 395 patients with peripheral arterial injuries, who were treated in the tertiary vascular university center in the period between 2005-2020. Exclusion criteria were isolated thoracic, abdominal and neck injuries as well as iatrogenic injuries and injuries of intravenous addicts. Univariate binary logistic regression analysis and multiple logistic regression were used to determine risk factors for lomb loss (after vascular reconstruction) or mortality. Decision to perform primary amputation (without vascular reconstruction) was based on surgeons' preference and experience. RESULTS: Out of 395 vascular injuries treated in the period 2005-2020, 210 (53.2%) presented with non-iatrogenic vascular injuries of upper and lower extremities were analyzed. According to the univariate regression analysis, hemorrhage as the main clinical manifestation on admission (P=0.035) and early reintervention (P=0.048) increased, while an early patency of repaired artery (0.010) significantly decreased a 30-day amputation rate. Multivariate logistic regression analysis of these three variables showed that only early patency of repaired artery significantly decreased the early amputation rate (P=0.009). CONCLUSIONS: Based on presented experience, the patency of vascular reconstruction plays a crucial role in limb salvage in patients with non-iatrogenic peripheral vascular injuries. All factors that might influence the patency should be in focus of improvement.
PB  - Edizioni Minerva Medica
T2  - The Journal of cardiovascular surgery
T1  - Treatment of vascular non-iatrogenic injuries of upper and lower extremities in tertiary vascular center
VL  - 64
IS  - 1
SP  - 74
EP  - 81
DO  - 10.23736/S0021-9509.22.12243-3
ER  - 
@article{
author = "Davidović, Lazar B. and Končar, Igor B. and Dragaš, Marko V. and Marković, Miroslav D. and Bogavac-Stanojević, Nataša and Vujčić, Aleksandra D. and Mitrović, Aleksandar C. and Ilić, Nikola S. and Trailović, R.D. and Kostić, Dušan M.",
abstract = "BACKGROUND: The purpose of this study was to examine demographic and baseline characteristics of patients with vascular injuries of extremities and to define the most relevant factors which influenced an early outcome, as well as limb salvage after the management of vascular trauma. METHODS: This study used the database that included 395 patients with peripheral arterial injuries, who were treated in the tertiary vascular university center in the period between 2005-2020. Exclusion criteria were isolated thoracic, abdominal and neck injuries as well as iatrogenic injuries and injuries of intravenous addicts. Univariate binary logistic regression analysis and multiple logistic regression were used to determine risk factors for lomb loss (after vascular reconstruction) or mortality. Decision to perform primary amputation (without vascular reconstruction) was based on surgeons' preference and experience. RESULTS: Out of 395 vascular injuries treated in the period 2005-2020, 210 (53.2%) presented with non-iatrogenic vascular injuries of upper and lower extremities were analyzed. According to the univariate regression analysis, hemorrhage as the main clinical manifestation on admission (P=0.035) and early reintervention (P=0.048) increased, while an early patency of repaired artery (0.010) significantly decreased a 30-day amputation rate. Multivariate logistic regression analysis of these three variables showed that only early patency of repaired artery significantly decreased the early amputation rate (P=0.009). CONCLUSIONS: Based on presented experience, the patency of vascular reconstruction plays a crucial role in limb salvage in patients with non-iatrogenic peripheral vascular injuries. All factors that might influence the patency should be in focus of improvement.",
publisher = "Edizioni Minerva Medica",
journal = "The Journal of cardiovascular surgery",
title = "Treatment of vascular non-iatrogenic injuries of upper and lower extremities in tertiary vascular center",
volume = "64",
number = "1",
pages = "74-81",
doi = "10.23736/S0021-9509.22.12243-3"
}
Davidović, L. B., Končar, I. B., Dragaš, M. V., Marković, M. D., Bogavac-Stanojević, N., Vujčić, A. D., Mitrović, A. C., Ilić, N. S., Trailović, R.D.,& Kostić, D. M..Treatment of vascular non-iatrogenic injuries of upper and lower extremities in tertiary vascular center. in The Journal of cardiovascular surgery
Edizioni Minerva Medica., 64(1), 74-81.
https://doi.org/10.23736/S0021-9509.22.12243-3
Davidović LB, Končar IB, Dragaš MV, Marković MD, Bogavac-Stanojević N, Vujčić AD, Mitrović AC, Ilić NS, Trailović R, Kostić DM. Treatment of vascular non-iatrogenic injuries of upper and lower extremities in tertiary vascular center. in The Journal of cardiovascular surgery.64(1):74-81.
doi:10.23736/S0021-9509.22.12243-3 .
Davidović, Lazar B., Končar, Igor B., Dragaš, Marko V., Marković, Miroslav D., Bogavac-Stanojević, Nataša, Vujčić, Aleksandra D., Mitrović, Aleksandar C., Ilić, Nikola S., Trailović, R.D., Kostić, Dušan M., "Treatment of vascular non-iatrogenic injuries of upper and lower extremities in tertiary vascular center" in The Journal of cardiovascular surgery, 64, no. 1:74-81,
https://doi.org/10.23736/S0021-9509.22.12243-3 . .

Rise of Pregabalin Poisoning and Abuse Cases in Serbia: A Ten-Year Retrospective Study

Antunović, Marko; Vučinić, Slavica; Kotur-Stevuljević, Jelena; Krstić, Kristijan; Jović-Stošić, Jasmina; Kilibarda, Vesna; Perković-Vukčević, Nataša; Đorđević, Snežana

TY  - JOUR
AU  - Antunović, Marko
AU  - Vučinić, Slavica
AU  - Kotur-Stevuljević, Jelena
AU  - Krstić, Kristijan
AU  - Jović-Stošić, Jasmina
AU  - Kilibarda, Vesna
AU  - Perković-Vukčević, Nataša
AU  - Đorđević, Snežana
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/4699
AB  - Purpose: The aim of this study was to analyze data on gabapentinoid-related attendances to the National Poison Control Center of Serbia (NPCC), particularly abuse cases; to estimate its changes and to compare it with trends in national consumption rates of these drugs. We also aimed to analyze the main characteristics of the study population and to investigate the major clinical effects in poisoned patients. Patients and Methods: This is a retrospective study of patients admitted to the NPCC for acute poisoning involving gabapentinoids from 1 May 2012 to 1 October 2022. Results: There were 357 (95.5%) pregabalin-related and 17 (4.5%) gabapentin-related poisoning cases in 302 patients. Abuse of pregabalin was detected in 27.8% (84/302), while gabapentin abuse occurred in 0.7% (2/302) of all patients. A steady increase in rates of pregabalin poisoning and abuse cases strongly correlated with the increase in overall consumption of this drug, while there were no significant changes in rates of gabapentin consumption, poisoning and abuse rate during the study period. Most patients who abused pregabalin pregabalin were males (84.5%) and the median age was 26 years (range: 15–45 years). Almost 60% of patients who abused pregabalin (48/84) belonged to the migrant population. Co-ingestions occurred in 89.4% of pregabalin-related cases (319/357), resulting in more severe poisoning. The most often co-ingested drugs were benzodiazepines and among them clonazepam was detected in the largest number of cases. Conclusion: The poisoning and abuse cases involving pregabalin are on the rise in Serbia, which coincided with an increase in its overall consumption during the study period. Isolated pregabalin ingestions resulted in mild poisoning, although severe symptoms such as coma and bradycardia were recorded. When prescribing pregabalin to patients at risk of abuse caution is needed. Strengthening the measures for dispensing of pregabalin may reduce the risks associated with its abuse.
PB  - Dove Medical Press Ltd
T2  - International Journal of General Medicine
T1  - Rise of Pregabalin Poisoning and Abuse Cases in Serbia: A Ten-Year Retrospective Study
VL  - 16
SP  - 1239
EP  - 1250
DO  - 10.2147/IJGM.S405616
ER  - 
@article{
author = "Antunović, Marko and Vučinić, Slavica and Kotur-Stevuljević, Jelena and Krstić, Kristijan and Jović-Stošić, Jasmina and Kilibarda, Vesna and Perković-Vukčević, Nataša and Đorđević, Snežana",
abstract = "Purpose: The aim of this study was to analyze data on gabapentinoid-related attendances to the National Poison Control Center of Serbia (NPCC), particularly abuse cases; to estimate its changes and to compare it with trends in national consumption rates of these drugs. We also aimed to analyze the main characteristics of the study population and to investigate the major clinical effects in poisoned patients. Patients and Methods: This is a retrospective study of patients admitted to the NPCC for acute poisoning involving gabapentinoids from 1 May 2012 to 1 October 2022. Results: There were 357 (95.5%) pregabalin-related and 17 (4.5%) gabapentin-related poisoning cases in 302 patients. Abuse of pregabalin was detected in 27.8% (84/302), while gabapentin abuse occurred in 0.7% (2/302) of all patients. A steady increase in rates of pregabalin poisoning and abuse cases strongly correlated with the increase in overall consumption of this drug, while there were no significant changes in rates of gabapentin consumption, poisoning and abuse rate during the study period. Most patients who abused pregabalin pregabalin were males (84.5%) and the median age was 26 years (range: 15–45 years). Almost 60% of patients who abused pregabalin (48/84) belonged to the migrant population. Co-ingestions occurred in 89.4% of pregabalin-related cases (319/357), resulting in more severe poisoning. The most often co-ingested drugs were benzodiazepines and among them clonazepam was detected in the largest number of cases. Conclusion: The poisoning and abuse cases involving pregabalin are on the rise in Serbia, which coincided with an increase in its overall consumption during the study period. Isolated pregabalin ingestions resulted in mild poisoning, although severe symptoms such as coma and bradycardia were recorded. When prescribing pregabalin to patients at risk of abuse caution is needed. Strengthening the measures for dispensing of pregabalin may reduce the risks associated with its abuse.",
publisher = "Dove Medical Press Ltd",
journal = "International Journal of General Medicine",
title = "Rise of Pregabalin Poisoning and Abuse Cases in Serbia: A Ten-Year Retrospective Study",
volume = "16",
pages = "1239-1250",
doi = "10.2147/IJGM.S405616"
}
Antunović, M., Vučinić, S., Kotur-Stevuljević, J., Krstić, K., Jović-Stošić, J., Kilibarda, V., Perković-Vukčević, N.,& Đorđević, S..Rise of Pregabalin Poisoning and Abuse Cases in Serbia: A Ten-Year Retrospective Study. in International Journal of General Medicine
Dove Medical Press Ltd., 16, 1239-1250.
https://doi.org/10.2147/IJGM.S405616
Antunović M, Vučinić S, Kotur-Stevuljević J, Krstić K, Jović-Stošić J, Kilibarda V, Perković-Vukčević N, Đorđević S. Rise of Pregabalin Poisoning and Abuse Cases in Serbia: A Ten-Year Retrospective Study. in International Journal of General Medicine.16:1239-1250.
doi:10.2147/IJGM.S405616 .
Antunović, Marko, Vučinić, Slavica, Kotur-Stevuljević, Jelena, Krstić, Kristijan, Jović-Stošić, Jasmina, Kilibarda, Vesna, Perković-Vukčević, Nataša, Đorđević, Snežana, "Rise of Pregabalin Poisoning and Abuse Cases in Serbia: A Ten-Year Retrospective Study" in International Journal of General Medicine, 16:1239-1250,
https://doi.org/10.2147/IJGM.S405616 . .
2
4
2

Trends in Anti-Tumor Effects of Pseudomonas aeruginosa Mannose-Sensitive-Hemagglutinin (PA-MSHA): An Overview of Positive and Negative Effects

Božić, Dragica; Živanović, Jovana; Živančević, Katarina; Baralić, Katarina; Đukić-Ćosić, Danijela

(MDPI, 2024)

TY  - JOUR
AU  - Božić, Dragica
AU  - Živanović, Jovana
AU  - Živančević, Katarina
AU  - Baralić, Katarina
AU  - Đukić-Ćosić, Danijela
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5531
AB  - Cancer is a leading cause of death worldwide, for which finding the optimal therapy remains an ongoing challenge. Drug resistance, toxic side effects, and a lack of specificity pose significant difficulties in traditional cancer treatments, leading to suboptimal clinical outcomes and high mortality rates among cancer patients. The need for alternative therapies is crucial, especially for those resistant to conventional methods like chemotherapy and radiotherapy or for patients where surgery is not possible. Over the past decade, a novel approach known as bacteria-mediated cancer therapy has emerged, offering potential solutions to the limitations of conventional treatments. An increasing number of in vitro and in vivo studies suggest that the subtype of highly virulent Pseudomonas aeruginosa bacterium called Pseudomonas aeruginosa mannose-sensitive-hemagglutinin (PA-MSHA) can successfully inhibit the progression of various cancer types, such as breast, lung, and bladder cancer, as well as hepatocellular carcinoma. PA-MSHA inhibits the growth and proliferation of tumor cells and induces their apoptosis. Proposed mechanisms of action include cell-cycle arrest and activation of pro-apoptotic pathways regulated by caspase-9 and caspase-3. Moreover, clinical studies have shown that PA-MSHA improved the effectiveness of chemotherapy and promoted the activation of the immune response in cancer patients without causing severe side effects. Reported adverse reactions were fever, skin irritation, and pain, attributed to the overactivation of the immune response. This review aims to summarize the current knowledge obtained from in vitro, in vivo, and clinical studies available at PubMed, Google Scholar, and ClinicalTrials.gov regarding the use of PA-MSHA in cancer treatment in order to further elucidate its pharmacological and toxicological properties.
PB  - MDPI
T2  - Cancers
T1  - Trends in Anti-Tumor Effects of Pseudomonas aeruginosa Mannose-Sensitive-Hemagglutinin (PA-MSHA): An Overview of Positive and Negative Effects
VL  - 16
IS  - 3
DO  - 10.3390/cancers16030524
ER  - 
@article{
author = "Božić, Dragica and Živanović, Jovana and Živančević, Katarina and Baralić, Katarina and Đukić-Ćosić, Danijela",
year = "2024",
abstract = "Cancer is a leading cause of death worldwide, for which finding the optimal therapy remains an ongoing challenge. Drug resistance, toxic side effects, and a lack of specificity pose significant difficulties in traditional cancer treatments, leading to suboptimal clinical outcomes and high mortality rates among cancer patients. The need for alternative therapies is crucial, especially for those resistant to conventional methods like chemotherapy and radiotherapy or for patients where surgery is not possible. Over the past decade, a novel approach known as bacteria-mediated cancer therapy has emerged, offering potential solutions to the limitations of conventional treatments. An increasing number of in vitro and in vivo studies suggest that the subtype of highly virulent Pseudomonas aeruginosa bacterium called Pseudomonas aeruginosa mannose-sensitive-hemagglutinin (PA-MSHA) can successfully inhibit the progression of various cancer types, such as breast, lung, and bladder cancer, as well as hepatocellular carcinoma. PA-MSHA inhibits the growth and proliferation of tumor cells and induces their apoptosis. Proposed mechanisms of action include cell-cycle arrest and activation of pro-apoptotic pathways regulated by caspase-9 and caspase-3. Moreover, clinical studies have shown that PA-MSHA improved the effectiveness of chemotherapy and promoted the activation of the immune response in cancer patients without causing severe side effects. Reported adverse reactions were fever, skin irritation, and pain, attributed to the overactivation of the immune response. This review aims to summarize the current knowledge obtained from in vitro, in vivo, and clinical studies available at PubMed, Google Scholar, and ClinicalTrials.gov regarding the use of PA-MSHA in cancer treatment in order to further elucidate its pharmacological and toxicological properties.",
publisher = "MDPI",
journal = "Cancers",
title = "Trends in Anti-Tumor Effects of Pseudomonas aeruginosa Mannose-Sensitive-Hemagglutinin (PA-MSHA): An Overview of Positive and Negative Effects",
volume = "16",
number = "3",
doi = "10.3390/cancers16030524"
}
Božić, D., Živanović, J., Živančević, K., Baralić, K.,& Đukić-Ćosić, D.. (2024). Trends in Anti-Tumor Effects of Pseudomonas aeruginosa Mannose-Sensitive-Hemagglutinin (PA-MSHA): An Overview of Positive and Negative Effects. in Cancers
MDPI., 16(3).
https://doi.org/10.3390/cancers16030524
Božić D, Živanović J, Živančević K, Baralić K, Đukić-Ćosić D. Trends in Anti-Tumor Effects of Pseudomonas aeruginosa Mannose-Sensitive-Hemagglutinin (PA-MSHA): An Overview of Positive and Negative Effects. in Cancers. 2024;16(3).
doi:10.3390/cancers16030524 .
Božić, Dragica, Živanović, Jovana, Živančević, Katarina, Baralić, Katarina, Đukić-Ćosić, Danijela, "Trends in Anti-Tumor Effects of Pseudomonas aeruginosa Mannose-Sensitive-Hemagglutinin (PA-MSHA): An Overview of Positive and Negative Effects" in Cancers, 16, no. 3 (2024),
https://doi.org/10.3390/cancers16030524 . .

New Imidazodiazepine Analogue, 5-(8-Bromo-6-(pyridin-2-yl)-4H-benzo[f]imidazo[1,5-a][1,4]diazepin-3-yl)oxazole, Provides a Simplified Synthetic Scheme, High Oral Plasma and Brain Exposures, and Produces Antiseizure Efficacy in Mice, and Antiepileptogenic Activity in Neural Networks in Brain Slices from a Patient with Mesial Temporal Lobe Epilepsy

Sharmin, Dishary; Divović, Branka; Ping, Xingjie; Cerne, Rok; Smith, Jodi L.; Rezvanian, Sepideh; Mondal, Prithu; Michelle, Meyer Jean; Kiley, Molly E.; Arnold, Leggy A.; Mian, Md Yeunus; Pandey, Kamal P.; Jin, Xiaoming; Mitrović, Jelena; Đorović, Đorđe; Lippa, Arnold; Cook, James M.; Golani, Lalit K.; Scholze, Petra; Savić, Miroslav; Witkin, Jeffrey M.

(American Chemical Society, 2024)

TY  - JOUR
AU  - Sharmin, Dishary
AU  - Divović, Branka
AU  - Ping, Xingjie
AU  - Cerne, Rok
AU  - Smith, Jodi L.
AU  - Rezvanian, Sepideh
AU  - Mondal, Prithu
AU  - Michelle, Meyer Jean
AU  - Kiley, Molly E.
AU  - Arnold, Leggy A.
AU  - Mian, Md Yeunus
AU  - Pandey, Kamal P.
AU  - Jin, Xiaoming
AU  - Mitrović, Jelena
AU  - Đorović, Đorđe
AU  - Lippa, Arnold
AU  - Cook, James M.
AU  - Golani, Lalit K.
AU  - Scholze, Petra
AU  - Savić, Miroslav
AU  - Witkin, Jeffrey M.
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5505
AB  - KRM-II-81 (1) is an imidazodiazepine GABAA receptor (GABAAR) potentiator with broad antiseizure efficacy and a low sedative burden. A brominated analogue, DS-II-73 (5), was synthesized and pharmacologically characterized as a potential backup compound as KRM-II-81 moves forward into development. The synthesis from 2-amino-5-bromophenyl)(pyridin-2yl)methanone (6) was processed in five steps with an overall yield of 38% and without the need for a palladium catalyst. GABAAR binding occurred with a Ki of 150 nM, and only 3 of 41 screened binding sites produced inhibition ≥50% at 10 μM, and the potency to induce cytotoxicity was ≥240 mM. DS-II-73 was selective for α2/3/5- over that of α1-containing GABAARs. Oral exposure of plasma and brain of rats was more than sufficient to functionally impact GABAARs. Tonic convulsions in mice and lethality induced by pentylenetetrazol were suppressed by DS-II-73 after oral administration and latencies to clonic and tonic seizures were prolonged. Cortical slice preparations from a patient with pharmacoresistant epilepsy (mesial temporal lobe) showed decreases in the frequency of local field potentials by DS-II-73. As with KRM-II-81, the motor-impairing effects of DS-II-73 were low compared to diazepam. Molecular docking studies of DS-II-73 with the α1β3γ2L-configured GABAAR showed low interaction with α1His102 that is suggested as a potential molecular mechanism for its low sedative side effects. These findings support the viability of DS-II-73 as a backup molecule for its ethynyl analogue, KRM-II-81, with the human tissue data providing translational credibility.
PB  - American Chemical Society
T2  - ACS Chemical Neuroscience
T1  - New Imidazodiazepine Analogue, 5-(8-Bromo-6-(pyridin-2-yl)-4H-benzo[f]imidazo[1,5-a][1,4]diazepin-3-yl)oxazole, Provides a Simplified Synthetic Scheme, High Oral Plasma and Brain Exposures, and Produces Antiseizure Efficacy in Mice, and Antiepileptogenic Activity in Neural Networks in Brain Slices from a Patient with Mesial Temporal Lobe Epilepsy
VL  - 15
IS  - 3
SP  - 517
EP  - 526
DO  - 10.1021/acschemneuro.3c00555
ER  - 
@article{
author = "Sharmin, Dishary and Divović, Branka and Ping, Xingjie and Cerne, Rok and Smith, Jodi L. and Rezvanian, Sepideh and Mondal, Prithu and Michelle, Meyer Jean and Kiley, Molly E. and Arnold, Leggy A. and Mian, Md Yeunus and Pandey, Kamal P. and Jin, Xiaoming and Mitrović, Jelena and Đorović, Đorđe and Lippa, Arnold and Cook, James M. and Golani, Lalit K. and Scholze, Petra and Savić, Miroslav and Witkin, Jeffrey M.",
year = "2024",
abstract = "KRM-II-81 (1) is an imidazodiazepine GABAA receptor (GABAAR) potentiator with broad antiseizure efficacy and a low sedative burden. A brominated analogue, DS-II-73 (5), was synthesized and pharmacologically characterized as a potential backup compound as KRM-II-81 moves forward into development. The synthesis from 2-amino-5-bromophenyl)(pyridin-2yl)methanone (6) was processed in five steps with an overall yield of 38% and without the need for a palladium catalyst. GABAAR binding occurred with a Ki of 150 nM, and only 3 of 41 screened binding sites produced inhibition ≥50% at 10 μM, and the potency to induce cytotoxicity was ≥240 mM. DS-II-73 was selective for α2/3/5- over that of α1-containing GABAARs. Oral exposure of plasma and brain of rats was more than sufficient to functionally impact GABAARs. Tonic convulsions in mice and lethality induced by pentylenetetrazol were suppressed by DS-II-73 after oral administration and latencies to clonic and tonic seizures were prolonged. Cortical slice preparations from a patient with pharmacoresistant epilepsy (mesial temporal lobe) showed decreases in the frequency of local field potentials by DS-II-73. As with KRM-II-81, the motor-impairing effects of DS-II-73 were low compared to diazepam. Molecular docking studies of DS-II-73 with the α1β3γ2L-configured GABAAR showed low interaction with α1His102 that is suggested as a potential molecular mechanism for its low sedative side effects. These findings support the viability of DS-II-73 as a backup molecule for its ethynyl analogue, KRM-II-81, with the human tissue data providing translational credibility.",
publisher = "American Chemical Society",
journal = "ACS Chemical Neuroscience",
title = "New Imidazodiazepine Analogue, 5-(8-Bromo-6-(pyridin-2-yl)-4H-benzo[f]imidazo[1,5-a][1,4]diazepin-3-yl)oxazole, Provides a Simplified Synthetic Scheme, High Oral Plasma and Brain Exposures, and Produces Antiseizure Efficacy in Mice, and Antiepileptogenic Activity in Neural Networks in Brain Slices from a Patient with Mesial Temporal Lobe Epilepsy",
volume = "15",
number = "3",
pages = "517-526",
doi = "10.1021/acschemneuro.3c00555"
}
Sharmin, D., Divović, B., Ping, X., Cerne, R., Smith, J. L., Rezvanian, S., Mondal, P., Michelle, M. J., Kiley, M. E., Arnold, L. A., Mian, M. Y., Pandey, K. P., Jin, X., Mitrović, J., Đorović, Đ., Lippa, A., Cook, J. M., Golani, L. K., Scholze, P., Savić, M.,& Witkin, J. M.. (2024). New Imidazodiazepine Analogue, 5-(8-Bromo-6-(pyridin-2-yl)-4H-benzo[f]imidazo[1,5-a][1,4]diazepin-3-yl)oxazole, Provides a Simplified Synthetic Scheme, High Oral Plasma and Brain Exposures, and Produces Antiseizure Efficacy in Mice, and Antiepileptogenic Activity in Neural Networks in Brain Slices from a Patient with Mesial Temporal Lobe Epilepsy. in ACS Chemical Neuroscience
American Chemical Society., 15(3), 517-526.
https://doi.org/10.1021/acschemneuro.3c00555
Sharmin D, Divović B, Ping X, Cerne R, Smith JL, Rezvanian S, Mondal P, Michelle MJ, Kiley ME, Arnold LA, Mian MY, Pandey KP, Jin X, Mitrović J, Đorović Đ, Lippa A, Cook JM, Golani LK, Scholze P, Savić M, Witkin JM. New Imidazodiazepine Analogue, 5-(8-Bromo-6-(pyridin-2-yl)-4H-benzo[f]imidazo[1,5-a][1,4]diazepin-3-yl)oxazole, Provides a Simplified Synthetic Scheme, High Oral Plasma and Brain Exposures, and Produces Antiseizure Efficacy in Mice, and Antiepileptogenic Activity in Neural Networks in Brain Slices from a Patient with Mesial Temporal Lobe Epilepsy. in ACS Chemical Neuroscience. 2024;15(3):517-526.
doi:10.1021/acschemneuro.3c00555 .
Sharmin, Dishary, Divović, Branka, Ping, Xingjie, Cerne, Rok, Smith, Jodi L., Rezvanian, Sepideh, Mondal, Prithu, Michelle, Meyer Jean, Kiley, Molly E., Arnold, Leggy A., Mian, Md Yeunus, Pandey, Kamal P., Jin, Xiaoming, Mitrović, Jelena, Đorović, Đorđe, Lippa, Arnold, Cook, James M., Golani, Lalit K., Scholze, Petra, Savić, Miroslav, Witkin, Jeffrey M., "New Imidazodiazepine Analogue, 5-(8-Bromo-6-(pyridin-2-yl)-4H-benzo[f]imidazo[1,5-a][1,4]diazepin-3-yl)oxazole, Provides a Simplified Synthetic Scheme, High Oral Plasma and Brain Exposures, and Produces Antiseizure Efficacy in Mice, and Antiepileptogenic Activity in Neural Networks in Brain Slices from a Patient with Mesial Temporal Lobe Epilepsy" in ACS Chemical Neuroscience, 15, no. 3 (2024):517-526,
https://doi.org/10.1021/acschemneuro.3c00555 . .

Cu(II) complexes with a salicylaldehyde derivative and α-diimines as co-ligands: synthesis, characterization, biological activity. Experimental and theoretical approach

Selaković, Snežana; Rodić, Marko; Novaković, Irena; Matić, Ivana; Stanojković, Tatjana; Pirković, Andrea; Živković, Lada; Spremo-Potparević, Biljana; Milčić, Vesna; Dimiza, Filitsa; Psomas, George; Anđelković, Katarina; Šumar-Ristović, Maja

(Royal Society of Chemistry, 2024)

TY  - JOUR
AU  - Selaković, Snežana
AU  - Rodić, Marko
AU  - Novaković, Irena
AU  - Matić, Ivana
AU  - Stanojković, Tatjana
AU  - Pirković, Andrea
AU  - Živković, Lada
AU  - Spremo-Potparević, Biljana
AU  - Milčić, Vesna
AU  - Dimiza, Filitsa
AU  - Psomas, George
AU  - Anđelković, Katarina
AU  - Šumar-Ristović, Maja
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5522
AB  - Copper(ii) complexes with an α-diimine show a wide variety of biological activities, such as antibacterial, antifungal, antioxidant and anticancer. In this work, we synthesized and structurally characterized two novel Cu(ii) complexes with methyl 3-formyl-4-hydroxybenzoate (HL) and α-diimines: 2,2′-bipyridine (bipy) and 1,10-phenanthroline (phen). Crystal structure analysis shows that the formulas of the compounds are [Cu(bipy)(L)(BF4)] (1) and [Cu(phen)(L)(H2O)](BF4)·H2O (2), with BF4− as a ligand in complex 1, which is rarely coordinated to metals. Both complexes have a square pyramidal geometry, while DFT calculations showed that the most stable structures of complexes 1 and 2 in a water/DMSO mixture are square-planar derivatives [Cu(bipy)(L)]+ and [Cu(phen)(L)]+. The antibacterial activity of compounds was evaluated in vitro on four Gram-negative and four Gram-positive bacterial strains. Complex 2 showed greater antibacterial activity towards all bacterial strains comparable to the control compound Amikacin. Complex 2 exerted a strong cytotoxic effect against the tested cancer cell lines (IC50 values ranging from 0.32 to 0.44 μM). Both complexes caused apoptotic cell death in HeLa cells and a noticeable in vitro antiangiogenic effect. In the concentration range of 5 to 100 μM, the complexes showed the absence of a genotoxic effect and displayed a protective effect against oxidative DNA damage induced by H2O2 in human peripheral blood cells. The interaction between the compounds and calf-thymus DNA was evaluated by diverse techniques suggesting a tight binding, which was also confirmed by molecular docking. In addition, it was found that the complexes bind tightly and reversibly to bovine and human serum albumin.
PB  - Royal Society of Chemistry
T2  - Dalton Transactions
T1  - Cu(II) complexes with a salicylaldehyde derivative and α-diimines as co-ligands: synthesis, characterization, biological activity. Experimental and theoretical approach
VL  - 53
IS  - 6
SP  - 2770
EP  - 2788
DO  - 10.1039/d3dt03862a
ER  - 
@article{
author = "Selaković, Snežana and Rodić, Marko and Novaković, Irena and Matić, Ivana and Stanojković, Tatjana and Pirković, Andrea and Živković, Lada and Spremo-Potparević, Biljana and Milčić, Vesna and Dimiza, Filitsa and Psomas, George and Anđelković, Katarina and Šumar-Ristović, Maja",
year = "2024",
abstract = "Copper(ii) complexes with an α-diimine show a wide variety of biological activities, such as antibacterial, antifungal, antioxidant and anticancer. In this work, we synthesized and structurally characterized two novel Cu(ii) complexes with methyl 3-formyl-4-hydroxybenzoate (HL) and α-diimines: 2,2′-bipyridine (bipy) and 1,10-phenanthroline (phen). Crystal structure analysis shows that the formulas of the compounds are [Cu(bipy)(L)(BF4)] (1) and [Cu(phen)(L)(H2O)](BF4)·H2O (2), with BF4− as a ligand in complex 1, which is rarely coordinated to metals. Both complexes have a square pyramidal geometry, while DFT calculations showed that the most stable structures of complexes 1 and 2 in a water/DMSO mixture are square-planar derivatives [Cu(bipy)(L)]+ and [Cu(phen)(L)]+. The antibacterial activity of compounds was evaluated in vitro on four Gram-negative and four Gram-positive bacterial strains. Complex 2 showed greater antibacterial activity towards all bacterial strains comparable to the control compound Amikacin. Complex 2 exerted a strong cytotoxic effect against the tested cancer cell lines (IC50 values ranging from 0.32 to 0.44 μM). Both complexes caused apoptotic cell death in HeLa cells and a noticeable in vitro antiangiogenic effect. In the concentration range of 5 to 100 μM, the complexes showed the absence of a genotoxic effect and displayed a protective effect against oxidative DNA damage induced by H2O2 in human peripheral blood cells. The interaction between the compounds and calf-thymus DNA was evaluated by diverse techniques suggesting a tight binding, which was also confirmed by molecular docking. In addition, it was found that the complexes bind tightly and reversibly to bovine and human serum albumin.",
publisher = "Royal Society of Chemistry",
journal = "Dalton Transactions",
title = "Cu(II) complexes with a salicylaldehyde derivative and α-diimines as co-ligands: synthesis, characterization, biological activity. Experimental and theoretical approach",
volume = "53",
number = "6",
pages = "2770-2788",
doi = "10.1039/d3dt03862a"
}
Selaković, S., Rodić, M., Novaković, I., Matić, I., Stanojković, T., Pirković, A., Živković, L., Spremo-Potparević, B., Milčić, V., Dimiza, F., Psomas, G., Anđelković, K.,& Šumar-Ristović, M.. (2024). Cu(II) complexes with a salicylaldehyde derivative and α-diimines as co-ligands: synthesis, characterization, biological activity. Experimental and theoretical approach. in Dalton Transactions
Royal Society of Chemistry., 53(6), 2770-2788.
https://doi.org/10.1039/d3dt03862a
Selaković S, Rodić M, Novaković I, Matić I, Stanojković T, Pirković A, Živković L, Spremo-Potparević B, Milčić V, Dimiza F, Psomas G, Anđelković K, Šumar-Ristović M. Cu(II) complexes with a salicylaldehyde derivative and α-diimines as co-ligands: synthesis, characterization, biological activity. Experimental and theoretical approach. in Dalton Transactions. 2024;53(6):2770-2788.
doi:10.1039/d3dt03862a .
Selaković, Snežana, Rodić, Marko, Novaković, Irena, Matić, Ivana, Stanojković, Tatjana, Pirković, Andrea, Živković, Lada, Spremo-Potparević, Biljana, Milčić, Vesna, Dimiza, Filitsa, Psomas, George, Anđelković, Katarina, Šumar-Ristović, Maja, "Cu(II) complexes with a salicylaldehyde derivative and α-diimines as co-ligands: synthesis, characterization, biological activity. Experimental and theoretical approach" in Dalton Transactions, 53, no. 6 (2024):2770-2788,
https://doi.org/10.1039/d3dt03862a . .
3

Maternal deprivation causes CaMKII downregulation and modulates glutamate, norepinephrine and serotonin in limbic brain areas in a rat model of single prolonged stress

Đorović, Đorđe; Lazarević, Vesna; Aranđelović, Jovana; Stevanović, Vladimir; Paslawski, Wojciech; Zhang, Xiaoqun; Velimirović, Milica; Petronijević, Nataša; Puškaš, Laslo; Savić, Miroslav; Svenningsson, Per

(Elsevier B.V., 2024)

TY  - JOUR
AU  - Đorović, Đorđe
AU  - Lazarević, Vesna
AU  - Aranđelović, Jovana
AU  - Stevanović, Vladimir
AU  - Paslawski, Wojciech
AU  - Zhang, Xiaoqun
AU  - Velimirović, Milica
AU  - Petronijević, Nataša
AU  - Puškaš, Laslo
AU  - Savić, Miroslav
AU  - Svenningsson, Per
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5503
AB  - Background: Early life stress is a major risk factor for later development of psychiatric disorders, including post-traumatic stress disorder (PTSD). An intricate relationship exists between various neurotransmitters (such as glutamate, norepinephrine or serotonin), calcium/calmodulin-dependent protein kinase II (CaMKII), as an important regulator of glutamatergic synaptic function, and PTSD. Here, we developed a double-hit model to investigate the interaction of maternal deprivation (MD) as an early life stress model and single prolonged stress (SPS) as a PTSD model at the behavioral and molecular levels. Methods: Male Wistar rats exposed to these stress paradigms were subjected to a comprehensive behavioral analysis. In hippocampal synaptosomes we investigated neurotransmitter release and glutamate concentration. The expression of CaMKII and the content of monoamines were determined in selected brain regions. Brain-derived neurotrophic factor (BDNF) mRNA was quantified by radioactive in situ hybridization. Results: We report a distinct behavioral phenotype in the double-hit group. Double-hit and SPS groups had decreased hippocampal presynaptic glutamatergic function. In hippocampus, double-hit stress caused a decrease in autophosphorylation of CaMKII. In prefrontal cortex, both SPS and double-hit stress had a similar effect on CaMKII autophosphorylation. Double-hit stress, rather than SPS, affected the norepinephrine and serotonin levels in prefrontal cortex, and suppressed BDNF gene expression in prefrontal cortex and hippocampus. Limitations: The study was conducted in male rats only. The affected brain regions cannot be restricted to hippocampus, prefrontal cortex and amygdala. Conclusion: Double-hit stress caused more pronounced and distinct behavioral, molecular and functional changes, compared to MD or SPS alone.
PB  - Elsevier B.V.
T2  - Journal of Affective Disorders
T1  - Maternal deprivation causes CaMKII downregulation and modulates glutamate, norepinephrine and serotonin in limbic brain areas in a rat model of single prolonged stress
VL  - 349
SP  - 286
EP  - 296
DO  - 10.1016/j.jad.2024.01.087
ER  - 
@article{
author = "Đorović, Đorđe and Lazarević, Vesna and Aranđelović, Jovana and Stevanović, Vladimir and Paslawski, Wojciech and Zhang, Xiaoqun and Velimirović, Milica and Petronijević, Nataša and Puškaš, Laslo and Savić, Miroslav and Svenningsson, Per",
year = "2024",
abstract = "Background: Early life stress is a major risk factor for later development of psychiatric disorders, including post-traumatic stress disorder (PTSD). An intricate relationship exists between various neurotransmitters (such as glutamate, norepinephrine or serotonin), calcium/calmodulin-dependent protein kinase II (CaMKII), as an important regulator of glutamatergic synaptic function, and PTSD. Here, we developed a double-hit model to investigate the interaction of maternal deprivation (MD) as an early life stress model and single prolonged stress (SPS) as a PTSD model at the behavioral and molecular levels. Methods: Male Wistar rats exposed to these stress paradigms were subjected to a comprehensive behavioral analysis. In hippocampal synaptosomes we investigated neurotransmitter release and glutamate concentration. The expression of CaMKII and the content of monoamines were determined in selected brain regions. Brain-derived neurotrophic factor (BDNF) mRNA was quantified by radioactive in situ hybridization. Results: We report a distinct behavioral phenotype in the double-hit group. Double-hit and SPS groups had decreased hippocampal presynaptic glutamatergic function. In hippocampus, double-hit stress caused a decrease in autophosphorylation of CaMKII. In prefrontal cortex, both SPS and double-hit stress had a similar effect on CaMKII autophosphorylation. Double-hit stress, rather than SPS, affected the norepinephrine and serotonin levels in prefrontal cortex, and suppressed BDNF gene expression in prefrontal cortex and hippocampus. Limitations: The study was conducted in male rats only. The affected brain regions cannot be restricted to hippocampus, prefrontal cortex and amygdala. Conclusion: Double-hit stress caused more pronounced and distinct behavioral, molecular and functional changes, compared to MD or SPS alone.",
publisher = "Elsevier B.V.",
journal = "Journal of Affective Disorders",
title = "Maternal deprivation causes CaMKII downregulation and modulates glutamate, norepinephrine and serotonin in limbic brain areas in a rat model of single prolonged stress",
volume = "349",
pages = "286-296",
doi = "10.1016/j.jad.2024.01.087"
}
Đorović, Đ., Lazarević, V., Aranđelović, J., Stevanović, V., Paslawski, W., Zhang, X., Velimirović, M., Petronijević, N., Puškaš, L., Savić, M.,& Svenningsson, P.. (2024). Maternal deprivation causes CaMKII downregulation and modulates glutamate, norepinephrine and serotonin in limbic brain areas in a rat model of single prolonged stress. in Journal of Affective Disorders
Elsevier B.V.., 349, 286-296.
https://doi.org/10.1016/j.jad.2024.01.087
Đorović Đ, Lazarević V, Aranđelović J, Stevanović V, Paslawski W, Zhang X, Velimirović M, Petronijević N, Puškaš L, Savić M, Svenningsson P. Maternal deprivation causes CaMKII downregulation and modulates glutamate, norepinephrine and serotonin in limbic brain areas in a rat model of single prolonged stress. in Journal of Affective Disorders. 2024;349:286-296.
doi:10.1016/j.jad.2024.01.087 .
Đorović, Đorđe, Lazarević, Vesna, Aranđelović, Jovana, Stevanović, Vladimir, Paslawski, Wojciech, Zhang, Xiaoqun, Velimirović, Milica, Petronijević, Nataša, Puškaš, Laslo, Savić, Miroslav, Svenningsson, Per, "Maternal deprivation causes CaMKII downregulation and modulates glutamate, norepinephrine and serotonin in limbic brain areas in a rat model of single prolonged stress" in Journal of Affective Disorders, 349 (2024):286-296,
https://doi.org/10.1016/j.jad.2024.01.087 . .

Novel chitosan and N-isopropylacrylamide-grafted-dextran-based microformulations as effective oral drug delivery system

Vučković, Nemanja; Prlainović, Nevena; Glođović, Nikola; Čalija, Bojan; Milosavljević, Nedeljko; Kalagasidis Krušić, Melina; Milašinović, Nikola

(2024)

TY  - JOUR
AU  - Vučković, Nemanja
AU  - Prlainović, Nevena
AU  - Glođović, Nikola
AU  - Čalija, Bojan
AU  - Milosavljević, Nedeljko
AU  - Kalagasidis Krušić, Melina
AU  - Milašinović, Nikola
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5504
AB  - Novel polymer network microformulations have been widely used for pharmaceutical applications. Especially challenging is to design and develop an ideal oral drug formulation due to many hostile factors in gastrointestinal (GI) tract microenvironment. Hydrogels attained striking attention for the use in controlled drug delivery systems, and for that purpose temperature- and pH-sensitive hydrogels have been extensively employed. This paper reports synthesis and characterization of innovative polymers-crosslinked hydrogel system consisted of N-isopropylacrylamide-graft-dextran (NiPAAm-g-Dex) and chitosan (Ch). Composition of the system was optimized to demonstrate distinguished encapsulation efficiency (EE) and release properties of diclofenac sodium (DS). The microspheres structure and morphology were confirmed by attenuated total reflectance Fourier transform infrared spectroscopy (ATR FT-IR) and scanning electron microscopy (SEM), respectively. Prepared microparticles have successfully passed through the simulated gastric and small intestine and reached the intestine, where the release of DS was carried out. In vitro release studies showed smooth release profile in a controllable manner with up to 40% release of the model drug after 4 h at pH 7.20 ± 0.01. Based on the results, novel polymer microformulations show excellent potential as controlled release drug delivery system and represent a superb candidate for additional in vivo testing.
T2  - Journal of the Iranian Chemical Society
T1  - Novel chitosan and N-isopropylacrylamide-grafted-dextran-based microformulations as effective oral drug delivery system
DO  - 10.1007/s13738-023-02960-1
ER  - 
@article{
author = "Vučković, Nemanja and Prlainović, Nevena and Glođović, Nikola and Čalija, Bojan and Milosavljević, Nedeljko and Kalagasidis Krušić, Melina and Milašinović, Nikola",
year = "2024",
abstract = "Novel polymer network microformulations have been widely used for pharmaceutical applications. Especially challenging is to design and develop an ideal oral drug formulation due to many hostile factors in gastrointestinal (GI) tract microenvironment. Hydrogels attained striking attention for the use in controlled drug delivery systems, and for that purpose temperature- and pH-sensitive hydrogels have been extensively employed. This paper reports synthesis and characterization of innovative polymers-crosslinked hydrogel system consisted of N-isopropylacrylamide-graft-dextran (NiPAAm-g-Dex) and chitosan (Ch). Composition of the system was optimized to demonstrate distinguished encapsulation efficiency (EE) and release properties of diclofenac sodium (DS). The microspheres structure and morphology were confirmed by attenuated total reflectance Fourier transform infrared spectroscopy (ATR FT-IR) and scanning electron microscopy (SEM), respectively. Prepared microparticles have successfully passed through the simulated gastric and small intestine and reached the intestine, where the release of DS was carried out. In vitro release studies showed smooth release profile in a controllable manner with up to 40% release of the model drug after 4 h at pH 7.20 ± 0.01. Based on the results, novel polymer microformulations show excellent potential as controlled release drug delivery system and represent a superb candidate for additional in vivo testing.",
journal = "Journal of the Iranian Chemical Society",
title = "Novel chitosan and N-isopropylacrylamide-grafted-dextran-based microformulations as effective oral drug delivery system",
doi = "10.1007/s13738-023-02960-1"
}
Vučković, N., Prlainović, N., Glođović, N., Čalija, B., Milosavljević, N., Kalagasidis Krušić, M.,& Milašinović, N.. (2024). Novel chitosan and N-isopropylacrylamide-grafted-dextran-based microformulations as effective oral drug delivery system. in Journal of the Iranian Chemical Society.
https://doi.org/10.1007/s13738-023-02960-1
Vučković N, Prlainović N, Glođović N, Čalija B, Milosavljević N, Kalagasidis Krušić M, Milašinović N. Novel chitosan and N-isopropylacrylamide-grafted-dextran-based microformulations as effective oral drug delivery system. in Journal of the Iranian Chemical Society. 2024;.
doi:10.1007/s13738-023-02960-1 .
Vučković, Nemanja, Prlainović, Nevena, Glođović, Nikola, Čalija, Bojan, Milosavljević, Nedeljko, Kalagasidis Krušić, Melina, Milašinović, Nikola, "Novel chitosan and N-isopropylacrylamide-grafted-dextran-based microformulations as effective oral drug delivery system" in Journal of the Iranian Chemical Society (2024),
https://doi.org/10.1007/s13738-023-02960-1 . .

Synthesis, Characterization, and Investigation of Anti-Inflammatory and Cytotoxic Activities of Novel Thiourea Derivatives of Naproxen

Nedeljković, Nikola; Nikolić, Miloš; Čanović, Petar; Zarić, Milan; Živković-Zarić, Radica; Bošković, Jelena; Vesović, Marina; Bradić, Jovana; Anđić, Marijana; Kočović, Aleksandar; Nikolić, Marina; Jakovljević, Vladimir; Vujić, Zorica; Dobričić, Vladimir

(MDPI, 2024)

TY  - JOUR
AU  - Nedeljković, Nikola
AU  - Nikolić, Miloš
AU  - Čanović, Petar
AU  - Zarić, Milan
AU  - Živković-Zarić, Radica
AU  - Bošković, Jelena
AU  - Vesović, Marina
AU  - Bradić, Jovana
AU  - Anđić, Marijana
AU  - Kočović, Aleksandar
AU  - Nikolić, Marina
AU  - Jakovljević, Vladimir
AU  - Vujić, Zorica
AU  - Dobričić, Vladimir
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5449
AB  - The objective of this study was to synthesize seven novel thiourea derivatives of naproxen (8–14), examine the anti-inflammatory activity of the newly synthesized compounds, investigate the cytotoxic potential of both sets of synthesized compounds (1–7 and 8–14), and select the most promising anti-inflammatory and antitumor drug candidates. The results of the in vivo anti-inflammatory study clearly showed that compounds 8 and 9 were capable of decreasing paw edema, as evident from a high percentage of inhibition (44.83% and 49.29%, respectively). In addition, the results of in vitro enzyme inhibition assays demonstrated that neither of the newly synthesized compounds reached 50% inhibition of 5-LOX at concentrations lower than 100 μM. In terms of antitumor potential, derivatives 3 and 8 exhibited strong cytotoxic effects on the HeLa cell line, suggesting the involvement of the extrinsic pathway of apoptosis. According to the overall results obtained for both sets of synthesized molecules, derivatives 4 and 8 can be underlined as molecules with the strongest anti-inflammatory activity, while derivatives 3 and 8 are the most promising cytotoxic agents.
PB  - MDPI
T2  - Pharmaceutics
T1  - Synthesis, Characterization, and Investigation of Anti-Inflammatory and Cytotoxic Activities of Novel Thiourea Derivatives of Naproxen
VL  - 16
IS  - 1
SP  - 1
DO  - 10.3390/pharmaceutics16010001
ER  - 
@article{
author = "Nedeljković, Nikola and Nikolić, Miloš and Čanović, Petar and Zarić, Milan and Živković-Zarić, Radica and Bošković, Jelena and Vesović, Marina and Bradić, Jovana and Anđić, Marijana and Kočović, Aleksandar and Nikolić, Marina and Jakovljević, Vladimir and Vujić, Zorica and Dobričić, Vladimir",
year = "2024",
abstract = "The objective of this study was to synthesize seven novel thiourea derivatives of naproxen (8–14), examine the anti-inflammatory activity of the newly synthesized compounds, investigate the cytotoxic potential of both sets of synthesized compounds (1–7 and 8–14), and select the most promising anti-inflammatory and antitumor drug candidates. The results of the in vivo anti-inflammatory study clearly showed that compounds 8 and 9 were capable of decreasing paw edema, as evident from a high percentage of inhibition (44.83% and 49.29%, respectively). In addition, the results of in vitro enzyme inhibition assays demonstrated that neither of the newly synthesized compounds reached 50% inhibition of 5-LOX at concentrations lower than 100 μM. In terms of antitumor potential, derivatives 3 and 8 exhibited strong cytotoxic effects on the HeLa cell line, suggesting the involvement of the extrinsic pathway of apoptosis. According to the overall results obtained for both sets of synthesized molecules, derivatives 4 and 8 can be underlined as molecules with the strongest anti-inflammatory activity, while derivatives 3 and 8 are the most promising cytotoxic agents.",
publisher = "MDPI",
journal = "Pharmaceutics",
title = "Synthesis, Characterization, and Investigation of Anti-Inflammatory and Cytotoxic Activities of Novel Thiourea Derivatives of Naproxen",
volume = "16",
number = "1",
pages = "1",
doi = "10.3390/pharmaceutics16010001"
}
Nedeljković, N., Nikolić, M., Čanović, P., Zarić, M., Živković-Zarić, R., Bošković, J., Vesović, M., Bradić, J., Anđić, M., Kočović, A., Nikolić, M., Jakovljević, V., Vujić, Z.,& Dobričić, V.. (2024). Synthesis, Characterization, and Investigation of Anti-Inflammatory and Cytotoxic Activities of Novel Thiourea Derivatives of Naproxen. in Pharmaceutics
MDPI., 16(1), 1.
https://doi.org/10.3390/pharmaceutics16010001
Nedeljković N, Nikolić M, Čanović P, Zarić M, Živković-Zarić R, Bošković J, Vesović M, Bradić J, Anđić M, Kočović A, Nikolić M, Jakovljević V, Vujić Z, Dobričić V. Synthesis, Characterization, and Investigation of Anti-Inflammatory and Cytotoxic Activities of Novel Thiourea Derivatives of Naproxen. in Pharmaceutics. 2024;16(1):1.
doi:10.3390/pharmaceutics16010001 .
Nedeljković, Nikola, Nikolić, Miloš, Čanović, Petar, Zarić, Milan, Živković-Zarić, Radica, Bošković, Jelena, Vesović, Marina, Bradić, Jovana, Anđić, Marijana, Kočović, Aleksandar, Nikolić, Marina, Jakovljević, Vladimir, Vujić, Zorica, Dobričić, Vladimir, "Synthesis, Characterization, and Investigation of Anti-Inflammatory and Cytotoxic Activities of Novel Thiourea Derivatives of Naproxen" in Pharmaceutics, 16, no. 1 (2024):1,
https://doi.org/10.3390/pharmaceutics16010001 . .
3

Endocrine disruptors in e-waste dismantling dust: In silico prediction of mixture-induced reproductive toxicity mechanisms

Radović, Biljana; Baralić, Katarina; Ćurčić, Marijana; Marić, Đurđica; Živanović, Jovana; Antonijević-Miljaković, Evica; Buha-Đorđević, Aleksandra; Đukić-Ćosić, Danijela; Bulat, Zorica; Antonijević, Biljana

(Elsevier V. B., 2024)

TY  - JOUR
AU  - Radović, Biljana
AU  - Baralić, Katarina
AU  - Ćurčić, Marijana
AU  - Marić, Đurđica
AU  - Živanović, Jovana
AU  - Antonijević-Miljaković, Evica
AU  - Buha-Đorđević, Aleksandra
AU  - Đukić-Ćosić, Danijela
AU  - Bulat, Zorica
AU  - Antonijević, Biljana
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5530
AB  - The constant exposure of humans to a mixture of low doses of toxic substances, emerging from the daily emission of toxic dust containing various metals and organic compounds in electrical and electronic waste (e-waste) recycling areas, poses potential harmful effects on health and the environment. While individually recognized as endocrine disruptors affecting hormonal balance, the combined impact of these toxic substances in a mixture remains insufficiently explored, particularly in relation to reproductive health. Thus, the aim of this in silico analysis was to: (i) assess the relationship between the exposure to a mixture of DBDE, DBDPE, TBBPA, Pb, Cd and Ni and development of male and female reproductive system disorders; and (ii) demonstrate the ability of in silico toxicogenomic tools in revealing the potential molecular mechanisms involved in the mixture toxicity. As the main data-mining tool, Comparative Toxicogenomics Database (CTD) was used, along with the ToppGene Suite portal and GeneMANIA online server. Our analysis identified 5 genes common to all the investigated substances and linked to reproductive system disorders. Notably, the most prominent interactions among these genes were physical interactions (77.64 %). Pathway enrichment analysis identified oxidative stress response as the central disrupted molecular pathway linked to reproductive pathology in the investigated mixture, while our chemical-phenotype CTD analysis uncovered additional affected pathways – apoptosis, hormonal regulation, and developmental functions. These findings highlight an increased risk of reproductive system disorders associated with the exposure to the investigated mixture of toxic substances in electronic waste recycling areas, emphasizing the urgent need for attention to address this environmental health concern. Hence, future laboratory studies should prioritize investigating the specific genes and common mechanisms identified in this study.
PB  - Elsevier V. B.
T2  - Science of the Total Environment
T1  - Endocrine disruptors in e-waste dismantling dust: In silico prediction of mixture-induced reproductive toxicity mechanisms
VL  - 917
SP  - 170437
DO  - 10.1016/j.scitotenv.2024.170437
ER  - 
@article{
author = "Radović, Biljana and Baralić, Katarina and Ćurčić, Marijana and Marić, Đurđica and Živanović, Jovana and Antonijević-Miljaković, Evica and Buha-Đorđević, Aleksandra and Đukić-Ćosić, Danijela and Bulat, Zorica and Antonijević, Biljana",
year = "2024",
abstract = "The constant exposure of humans to a mixture of low doses of toxic substances, emerging from the daily emission of toxic dust containing various metals and organic compounds in electrical and electronic waste (e-waste) recycling areas, poses potential harmful effects on health and the environment. While individually recognized as endocrine disruptors affecting hormonal balance, the combined impact of these toxic substances in a mixture remains insufficiently explored, particularly in relation to reproductive health. Thus, the aim of this in silico analysis was to: (i) assess the relationship between the exposure to a mixture of DBDE, DBDPE, TBBPA, Pb, Cd and Ni and development of male and female reproductive system disorders; and (ii) demonstrate the ability of in silico toxicogenomic tools in revealing the potential molecular mechanisms involved in the mixture toxicity. As the main data-mining tool, Comparative Toxicogenomics Database (CTD) was used, along with the ToppGene Suite portal and GeneMANIA online server. Our analysis identified 5 genes common to all the investigated substances and linked to reproductive system disorders. Notably, the most prominent interactions among these genes were physical interactions (77.64 %). Pathway enrichment analysis identified oxidative stress response as the central disrupted molecular pathway linked to reproductive pathology in the investigated mixture, while our chemical-phenotype CTD analysis uncovered additional affected pathways – apoptosis, hormonal regulation, and developmental functions. These findings highlight an increased risk of reproductive system disorders associated with the exposure to the investigated mixture of toxic substances in electronic waste recycling areas, emphasizing the urgent need for attention to address this environmental health concern. Hence, future laboratory studies should prioritize investigating the specific genes and common mechanisms identified in this study.",
publisher = "Elsevier V. B.",
journal = "Science of the Total Environment",
title = "Endocrine disruptors in e-waste dismantling dust: In silico prediction of mixture-induced reproductive toxicity mechanisms",
volume = "917",
pages = "170437",
doi = "10.1016/j.scitotenv.2024.170437"
}
Radović, B., Baralić, K., Ćurčić, M., Marić, Đ., Živanović, J., Antonijević-Miljaković, E., Buha-Đorđević, A., Đukić-Ćosić, D., Bulat, Z.,& Antonijević, B.. (2024). Endocrine disruptors in e-waste dismantling dust: In silico prediction of mixture-induced reproductive toxicity mechanisms. in Science of the Total Environment
Elsevier V. B.., 917, 170437.
https://doi.org/10.1016/j.scitotenv.2024.170437
Radović B, Baralić K, Ćurčić M, Marić Đ, Živanović J, Antonijević-Miljaković E, Buha-Đorđević A, Đukić-Ćosić D, Bulat Z, Antonijević B. Endocrine disruptors in e-waste dismantling dust: In silico prediction of mixture-induced reproductive toxicity mechanisms. in Science of the Total Environment. 2024;917:170437.
doi:10.1016/j.scitotenv.2024.170437 .
Radović, Biljana, Baralić, Katarina, Ćurčić, Marijana, Marić, Đurđica, Živanović, Jovana, Antonijević-Miljaković, Evica, Buha-Đorđević, Aleksandra, Đukić-Ćosić, Danijela, Bulat, Zorica, Antonijević, Biljana, "Endocrine disruptors in e-waste dismantling dust: In silico prediction of mixture-induced reproductive toxicity mechanisms" in Science of the Total Environment, 917 (2024):170437,
https://doi.org/10.1016/j.scitotenv.2024.170437 . .
1

Therapeutic drug monitoring of monoclonal antibodies in chronic inflammatory diseases: A snapshot of laboratories and applications across Europe

Skrede, Silje; Bogavac-Stanojević, Nataša; Dreesen, Erwin; Nielsen, Elisabet; Zaninotto, Martina; Mulleman, Denis

(John Wiley and Sons Inc, 2024)

TY  - JOUR
AU  - Skrede, Silje
AU  - Bogavac-Stanojević, Nataša
AU  - Dreesen, Erwin
AU  - Nielsen, Elisabet
AU  - Zaninotto, Martina
AU  - Mulleman, Denis
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5532
AB  - The European Cooperation in Science and Technology (COST) action ENOTTA (The European Network on Optimising Treatment with Therapeutic Antibodies in chronic inflammatory diseases) was launched in 2022. To pave the way for harmonization of analytical methods for quantitation of serum levels of therapeutic antibodies in research and clinical settings, ENOTTA recently performed an online survey mapping laboratories in the field. The survey, which contained 30 questions surrounding therapeutic drug monitoring of relevant drugs and anti-drug antibodies, was distributed via the ENOTTA and European Federation of Clinical Chemistry and Laboratory networks. Among 63 respondents across Europe, 45 reported analytical activity, with a range of utilized methods. Future engagement of as many sites as possible will enable comparison of methodologies and facilitate progress in the field.
PB  - John Wiley and Sons Inc
T2  - Basic and Clinical Pharmacology and Toxicology
T1  - Therapeutic drug monitoring of monoclonal antibodies in chronic inflammatory diseases: A snapshot of laboratories and applications across Europe
DO  - 10.1111/bcpt.13983
ER  - 
@article{
author = "Skrede, Silje and Bogavac-Stanojević, Nataša and Dreesen, Erwin and Nielsen, Elisabet and Zaninotto, Martina and Mulleman, Denis",
year = "2024",
abstract = "The European Cooperation in Science and Technology (COST) action ENOTTA (The European Network on Optimising Treatment with Therapeutic Antibodies in chronic inflammatory diseases) was launched in 2022. To pave the way for harmonization of analytical methods for quantitation of serum levels of therapeutic antibodies in research and clinical settings, ENOTTA recently performed an online survey mapping laboratories in the field. The survey, which contained 30 questions surrounding therapeutic drug monitoring of relevant drugs and anti-drug antibodies, was distributed via the ENOTTA and European Federation of Clinical Chemistry and Laboratory networks. Among 63 respondents across Europe, 45 reported analytical activity, with a range of utilized methods. Future engagement of as many sites as possible will enable comparison of methodologies and facilitate progress in the field.",
publisher = "John Wiley and Sons Inc",
journal = "Basic and Clinical Pharmacology and Toxicology",
title = "Therapeutic drug monitoring of monoclonal antibodies in chronic inflammatory diseases: A snapshot of laboratories and applications across Europe",
doi = "10.1111/bcpt.13983"
}
Skrede, S., Bogavac-Stanojević, N., Dreesen, E., Nielsen, E., Zaninotto, M.,& Mulleman, D.. (2024). Therapeutic drug monitoring of monoclonal antibodies in chronic inflammatory diseases: A snapshot of laboratories and applications across Europe. in Basic and Clinical Pharmacology and Toxicology
John Wiley and Sons Inc..
https://doi.org/10.1111/bcpt.13983
Skrede S, Bogavac-Stanojević N, Dreesen E, Nielsen E, Zaninotto M, Mulleman D. Therapeutic drug monitoring of monoclonal antibodies in chronic inflammatory diseases: A snapshot of laboratories and applications across Europe. in Basic and Clinical Pharmacology and Toxicology. 2024;.
doi:10.1111/bcpt.13983 .
Skrede, Silje, Bogavac-Stanojević, Nataša, Dreesen, Erwin, Nielsen, Elisabet, Zaninotto, Martina, Mulleman, Denis, "Therapeutic drug monitoring of monoclonal antibodies in chronic inflammatory diseases: A snapshot of laboratories and applications across Europe" in Basic and Clinical Pharmacology and Toxicology (2024),
https://doi.org/10.1111/bcpt.13983 . .

Chemical Analysis and Investigation of Antimicrobial and Antibiofilm Activities of Prangos trifida (Apiaceae)

Ušjak, Ljuboš; Stojković, Dejan; Carević, Tamara; Milutinović, Violeta; Soković, Marina; Niketić, Marjan; Petrović, Silvana

(MDPI, 2024)

TY  - JOUR
AU  - Ušjak, Ljuboš
AU  - Stojković, Dejan
AU  - Carević, Tamara
AU  - Milutinović, Violeta
AU  - Soković, Marina
AU  - Niketić, Marjan
AU  - Petrović, Silvana
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5417
AB  - Plants of the genus Prangos are intensively investigated as potential new sources of bioactive isolated products. In this work, the chemical composition of volatile constituents (essential oils and headspace volatiles) and dichloromethane extracts, as well as antimicrobial and antibiofilm activities of essential oils and MFDEs (methanol fractions of dichloromethane extracts) of Prangos trifida from Serbia, were investigated. Volatiles of roots, leaves, stems and fruits, and fatty acids and phytosterols in dichloromethane extracts of roots and fruits were analyzed by GC-FID-MS, whereas coumarins in MFDEs by LC–MS and some isolated coumarins by 1H-NMR. Minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations/minimum fungicidal concentrations (MBCs/MFCs) of essential oils and MFDEs were determined against 13 microorganisms. Antibiofilm activity was assessed against four microorganisms. Additionally, congo red and ergosterol binding assays were conducted to elucidate selected mechanisms of antibiofilm action in the case of Candida albicans. Total of 52 volatile constituents, 16 fatty acids, eight phytosterols and 10 coumarins were identified. Essential oils demonstrated significant activity, surpassing that of commercial food preservatives, against six tested molds from the Aspergillus, Penicillium and Trichoderma genera, as well as against bacteria Staphylococcus aureus and Bacillus cereus. Most of the oils strongly inhibited the formation of biofilms by S. aureus, Listeria monocytogenes and Escherichia coli. MFDEs exhibited noteworthy effects against B. cereus and the tested Aspergillus species, particularly A. niger, and significantly inhibited C. albicans biofilm formation. This inhibition was linked to a marked reduction in exopolysaccharide production, while antifungal mechanisms associated with ergosterol remained unaffected.
PB  - MDPI
T2  - Antibiotics
T1  - Chemical Analysis and Investigation of Antimicrobial and Antibiofilm Activities of Prangos trifida (Apiaceae)
VL  - 13
IS  - 1
SP  - 41
DO  - 10.3390/antibiotics13010041
ER  - 
@article{
author = "Ušjak, Ljuboš and Stojković, Dejan and Carević, Tamara and Milutinović, Violeta and Soković, Marina and Niketić, Marjan and Petrović, Silvana",
year = "2024",
abstract = "Plants of the genus Prangos are intensively investigated as potential new sources of bioactive isolated products. In this work, the chemical composition of volatile constituents (essential oils and headspace volatiles) and dichloromethane extracts, as well as antimicrobial and antibiofilm activities of essential oils and MFDEs (methanol fractions of dichloromethane extracts) of Prangos trifida from Serbia, were investigated. Volatiles of roots, leaves, stems and fruits, and fatty acids and phytosterols in dichloromethane extracts of roots and fruits were analyzed by GC-FID-MS, whereas coumarins in MFDEs by LC–MS and some isolated coumarins by 1H-NMR. Minimum inhibitory concentrations (MICs) and minimum bactericidal concentrations/minimum fungicidal concentrations (MBCs/MFCs) of essential oils and MFDEs were determined against 13 microorganisms. Antibiofilm activity was assessed against four microorganisms. Additionally, congo red and ergosterol binding assays were conducted to elucidate selected mechanisms of antibiofilm action in the case of Candida albicans. Total of 52 volatile constituents, 16 fatty acids, eight phytosterols and 10 coumarins were identified. Essential oils demonstrated significant activity, surpassing that of commercial food preservatives, against six tested molds from the Aspergillus, Penicillium and Trichoderma genera, as well as against bacteria Staphylococcus aureus and Bacillus cereus. Most of the oils strongly inhibited the formation of biofilms by S. aureus, Listeria monocytogenes and Escherichia coli. MFDEs exhibited noteworthy effects against B. cereus and the tested Aspergillus species, particularly A. niger, and significantly inhibited C. albicans biofilm formation. This inhibition was linked to a marked reduction in exopolysaccharide production, while antifungal mechanisms associated with ergosterol remained unaffected.",
publisher = "MDPI",
journal = "Antibiotics",
title = "Chemical Analysis and Investigation of Antimicrobial and Antibiofilm Activities of Prangos trifida (Apiaceae)",
volume = "13",
number = "1",
pages = "41",
doi = "10.3390/antibiotics13010041"
}
Ušjak, L., Stojković, D., Carević, T., Milutinović, V., Soković, M., Niketić, M.,& Petrović, S.. (2024). Chemical Analysis and Investigation of Antimicrobial and Antibiofilm Activities of Prangos trifida (Apiaceae). in Antibiotics
MDPI., 13(1), 41.
https://doi.org/10.3390/antibiotics13010041
Ušjak L, Stojković D, Carević T, Milutinović V, Soković M, Niketić M, Petrović S. Chemical Analysis and Investigation of Antimicrobial and Antibiofilm Activities of Prangos trifida (Apiaceae). in Antibiotics. 2024;13(1):41.
doi:10.3390/antibiotics13010041 .
Ušjak, Ljuboš, Stojković, Dejan, Carević, Tamara, Milutinović, Violeta, Soković, Marina, Niketić, Marjan, Petrović, Silvana, "Chemical Analysis and Investigation of Antimicrobial and Antibiofilm Activities of Prangos trifida (Apiaceae)" in Antibiotics, 13, no. 1 (2024):41,
https://doi.org/10.3390/antibiotics13010041 . .

Composition of Essential Oils from Fruits of Peucedanum longifolium and Rhizomatophora aegopodioides (Apiaceae) with Regard to Other Related Taxa—A Chemometric Approach

Ušjak, Ljuboš; Niketić, Marjan; Petrović, Silvana

(MDPI, 2024)

TY  - JOUR
AU  - Ušjak, Ljuboš
AU  - Niketić, Marjan
AU  - Petrović, Silvana
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5418
AB  - The aim of this work was to investigate the composition of essential oils isolated from fruits of Peucedanum longifolium, and Rhizomatophora aegopodioides (a species which was previously placed in the genus Peucedanum), as well as to compare the obtained results to those available for other previously investigated related species (including taxa which are also, according to some authors, excluded from the genus Peucedanum). Essential oils were obtained via hydrodistillation in a Clevenger-type apparatus and their composition was analyzed using GC-FID and GC-MS. To compare these data to those of previously investigated taxa, a chemometric approach was applied; the data were analyzed using multivariate statistical methods: non-metric multidimensional scaling (nMDS) and hierarchical cluster analysis. The most abundant in P. longifolium essential oil were monoterpenes (79.7%), mostly α-phellandrene (26.2%), β-phellandrene + limonene (21.0%) and myrcene (9.5%), followed by sesquiterpenes (18.3%), mostly germacrene B (9.5%). On the other hand, dominant in R. aegopodioides essential oil were non-terpenic aliphatic hydrocarbons (46.1%), mainly n-undecane (16.5%) and n-nonane (11.3%). In addition, this essential oil also contained a notable quantity of sesquiterpenes (25.1%), with (E)-sesquilavandulol being the most abundant (10.0%). The results of multivariate statistics revealed a clear separation of the essential oil composition of R. aegopodioides and P. longifolium, as well as of P. longifolium and P. officinale. The clustering of the samples of most of the taxa that do not belong to the Peucedanum in the narrow sense (sensu stricto) was also observed, which is in accordance with their recent inclusion in separate genera.
PB  - MDPI
T2  - Separations
T1  - Composition of Essential Oils from Fruits of Peucedanum longifolium and Rhizomatophora aegopodioides (Apiaceae) with Regard to Other Related Taxa—A Chemometric Approach
VL  - 11
IS  - 1
SP  - 14
DO  - 10.3390/separations11010014
ER  - 
@article{
author = "Ušjak, Ljuboš and Niketić, Marjan and Petrović, Silvana",
year = "2024",
abstract = "The aim of this work was to investigate the composition of essential oils isolated from fruits of Peucedanum longifolium, and Rhizomatophora aegopodioides (a species which was previously placed in the genus Peucedanum), as well as to compare the obtained results to those available for other previously investigated related species (including taxa which are also, according to some authors, excluded from the genus Peucedanum). Essential oils were obtained via hydrodistillation in a Clevenger-type apparatus and their composition was analyzed using GC-FID and GC-MS. To compare these data to those of previously investigated taxa, a chemometric approach was applied; the data were analyzed using multivariate statistical methods: non-metric multidimensional scaling (nMDS) and hierarchical cluster analysis. The most abundant in P. longifolium essential oil were monoterpenes (79.7%), mostly α-phellandrene (26.2%), β-phellandrene + limonene (21.0%) and myrcene (9.5%), followed by sesquiterpenes (18.3%), mostly germacrene B (9.5%). On the other hand, dominant in R. aegopodioides essential oil were non-terpenic aliphatic hydrocarbons (46.1%), mainly n-undecane (16.5%) and n-nonane (11.3%). In addition, this essential oil also contained a notable quantity of sesquiterpenes (25.1%), with (E)-sesquilavandulol being the most abundant (10.0%). The results of multivariate statistics revealed a clear separation of the essential oil composition of R. aegopodioides and P. longifolium, as well as of P. longifolium and P. officinale. The clustering of the samples of most of the taxa that do not belong to the Peucedanum in the narrow sense (sensu stricto) was also observed, which is in accordance with their recent inclusion in separate genera.",
publisher = "MDPI",
journal = "Separations",
title = "Composition of Essential Oils from Fruits of Peucedanum longifolium and Rhizomatophora aegopodioides (Apiaceae) with Regard to Other Related Taxa—A Chemometric Approach",
volume = "11",
number = "1",
pages = "14",
doi = "10.3390/separations11010014"
}
Ušjak, L., Niketić, M.,& Petrović, S.. (2024). Composition of Essential Oils from Fruits of Peucedanum longifolium and Rhizomatophora aegopodioides (Apiaceae) with Regard to Other Related Taxa—A Chemometric Approach. in Separations
MDPI., 11(1), 14.
https://doi.org/10.3390/separations11010014
Ušjak L, Niketić M, Petrović S. Composition of Essential Oils from Fruits of Peucedanum longifolium and Rhizomatophora aegopodioides (Apiaceae) with Regard to Other Related Taxa—A Chemometric Approach. in Separations. 2024;11(1):14.
doi:10.3390/separations11010014 .
Ušjak, Ljuboš, Niketić, Marjan, Petrović, Silvana, "Composition of Essential Oils from Fruits of Peucedanum longifolium and Rhizomatophora aegopodioides (Apiaceae) with Regard to Other Related Taxa—A Chemometric Approach" in Separations, 11, no. 1 (2024):14,
https://doi.org/10.3390/separations11010014 . .

Sulforaphane—A Compound with Potential Health Benefits for Disease Prevention and Treatment: Insights from Pharmacological and Toxicological Experimental Studies

Baralić, Katarina; Živanović, Jovana; Marić, Đurđica; Božić, Dragica; Grahovac, Lazar; Antonijević-Miljaković, Evica; Ćurčić, Marijana; Buha-Đorđević, Aleksandra; Bulat, Zorica; Antonijević, Biljana; Đukić-Ćosić, Danijela

(MDPI, 2024)

TY  - JOUR
AU  - Baralić, Katarina
AU  - Živanović, Jovana
AU  - Marić, Đurđica
AU  - Božić, Dragica
AU  - Grahovac, Lazar
AU  - Antonijević-Miljaković, Evica
AU  - Ćurčić, Marijana
AU  - Buha-Đorđević, Aleksandra
AU  - Bulat, Zorica
AU  - Antonijević, Biljana
AU  - Đukić-Ćosić, Danijela
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5516
AB  - Sulforaphane (SFN), which is a hydrolysis product from glucoraphanin, a compound found in cruciferous vegetables, has been studied for its potential health benefits, particularly in disease prevention and treatment. SFN has proven to be effective in combating different types of cancer by inhibiting the proliferation of tumors and triggering apoptosis. This dual action has been demonstrated to result in a reduction in tumor size and an enhancement of survival rates in animal models. SFN has also shown antidiabetic and anti-obesity effects, improving glucose tolerance and reducing fat accumulation. SFN’s ability to activate Nrf2, a transcription factor regulating oxidative stress and inflammation in cells, is a primary mechanism behind its anticancerogenic and antidiabetic effects. Its antioxidant, anti-inflammatory, and anti-apoptotic properties are also suggested to provide beneficial effects against neurodegenerative diseases. The potential health benefits of SFN have led to increased interest in its use as a dietary supplement or adjunct to chemotherapy, but there are insufficient data on its efficacy and optimal doses, as well as its safety. This review aims to present and discuss SFN’s potential in treating various diseases, such as cancer, diabetes, cardiovascular diseases, obesity, and neurodegenerative diseases, focusing on its mechanisms of action. It also summarizes studies on the pharmacological and toxicological potential of SFN in in vitro and animal models and explores its protective role against toxic compounds through in vitro and animal studies.
PB  - MDPI
T2  - Antioxidants
T1  - Sulforaphane—A Compound with Potential Health Benefits for Disease Prevention and Treatment: Insights from Pharmacological and Toxicological Experimental Studies
VL  - 13
IS  - 2
DO  - 10.3390/antiox13020147
ER  - 
@article{
author = "Baralić, Katarina and Živanović, Jovana and Marić, Đurđica and Božić, Dragica and Grahovac, Lazar and Antonijević-Miljaković, Evica and Ćurčić, Marijana and Buha-Đorđević, Aleksandra and Bulat, Zorica and Antonijević, Biljana and Đukić-Ćosić, Danijela",
year = "2024",
abstract = "Sulforaphane (SFN), which is a hydrolysis product from glucoraphanin, a compound found in cruciferous vegetables, has been studied for its potential health benefits, particularly in disease prevention and treatment. SFN has proven to be effective in combating different types of cancer by inhibiting the proliferation of tumors and triggering apoptosis. This dual action has been demonstrated to result in a reduction in tumor size and an enhancement of survival rates in animal models. SFN has also shown antidiabetic and anti-obesity effects, improving glucose tolerance and reducing fat accumulation. SFN’s ability to activate Nrf2, a transcription factor regulating oxidative stress and inflammation in cells, is a primary mechanism behind its anticancerogenic and antidiabetic effects. Its antioxidant, anti-inflammatory, and anti-apoptotic properties are also suggested to provide beneficial effects against neurodegenerative diseases. The potential health benefits of SFN have led to increased interest in its use as a dietary supplement or adjunct to chemotherapy, but there are insufficient data on its efficacy and optimal doses, as well as its safety. This review aims to present and discuss SFN’s potential in treating various diseases, such as cancer, diabetes, cardiovascular diseases, obesity, and neurodegenerative diseases, focusing on its mechanisms of action. It also summarizes studies on the pharmacological and toxicological potential of SFN in in vitro and animal models and explores its protective role against toxic compounds through in vitro and animal studies.",
publisher = "MDPI",
journal = "Antioxidants",
title = "Sulforaphane—A Compound with Potential Health Benefits for Disease Prevention and Treatment: Insights from Pharmacological and Toxicological Experimental Studies",
volume = "13",
number = "2",
doi = "10.3390/antiox13020147"
}
Baralić, K., Živanović, J., Marić, Đ., Božić, D., Grahovac, L., Antonijević-Miljaković, E., Ćurčić, M., Buha-Đorđević, A., Bulat, Z., Antonijević, B.,& Đukić-Ćosić, D.. (2024). Sulforaphane—A Compound with Potential Health Benefits for Disease Prevention and Treatment: Insights from Pharmacological and Toxicological Experimental Studies. in Antioxidants
MDPI., 13(2).
https://doi.org/10.3390/antiox13020147
Baralić K, Živanović J, Marić Đ, Božić D, Grahovac L, Antonijević-Miljaković E, Ćurčić M, Buha-Đorđević A, Bulat Z, Antonijević B, Đukić-Ćosić D. Sulforaphane—A Compound with Potential Health Benefits for Disease Prevention and Treatment: Insights from Pharmacological and Toxicological Experimental Studies. in Antioxidants. 2024;13(2).
doi:10.3390/antiox13020147 .
Baralić, Katarina, Živanović, Jovana, Marić, Đurđica, Božić, Dragica, Grahovac, Lazar, Antonijević-Miljaković, Evica, Ćurčić, Marijana, Buha-Đorđević, Aleksandra, Bulat, Zorica, Antonijević, Biljana, Đukić-Ćosić, Danijela, "Sulforaphane—A Compound with Potential Health Benefits for Disease Prevention and Treatment: Insights from Pharmacological and Toxicological Experimental Studies" in Antioxidants, 13, no. 2 (2024),
https://doi.org/10.3390/antiox13020147 . .
1

Metal-induced autoimmunity in neurological disorders: A review of current understanding and future directions

Bjørklund, Geir; Buha-Đorđević, Aleksandra; Hamdan, Halla; Wallace, David R.; Peana, Massimiliano

(Elsevier B.V., 2024)

TY  - JOUR
AU  - Bjørklund, Geir
AU  - Buha-Đorđević, Aleksandra
AU  - Hamdan, Halla
AU  - Wallace, David R.
AU  - Peana, Massimiliano
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5443
AB  - Autoimmunity is a multifaceted disorder influenced by both genetic and environmental factors, and metal exposure has been implicated as a potential catalyst, especially in autoimmune diseases affecting the central nervous system. Notably, metals like mercury, lead, and aluminum exhibit well-established neurotoxic effects, yet the precise mechanisms by which they elicit autoimmune responses in susceptible individuals remain unclear. Recent studies propose that metal-induced autoimmunity may arise from direct toxic effects on immune cells and tissues, coupled with indirect impacts on the gut microbiome and the blood-brain barrier. These effects can activate self-reactive T cells, prompting the production of autoantibodies, inflammatory responses, and tissue damage. Diagnosing metal-induced autoimmunity proves challenging due to nonspecific symptoms and a lack of reliable biomarkers. Treatment typically involves chelation therapy to eliminate excess metals and immunomodulatory agents to suppress autoimmune responses. Prevention strategies include lifestyle adjustments to reduce metal exposure and avoiding occupational and environmental risks. Prognosis is generally favorable with proper treatment; however, untreated cases may lead to autoimmune disorder progression and irreversible organ damage, particularly in the brain. Future research aims to identify genetic and environmental risk factors, enhance diagnostic precision, and explore novel treatment approaches for improved prevention and management of this intricate and debilitating disease.
PB  - Elsevier B.V.
T2  - Autoimmunity Reviews
T1  - Metal-induced autoimmunity in neurological disorders: A review of current understanding and future directions
VL  - 23
IS  - 3
DO  - 10.1016/j.autrev.2023.103509
DO  - 2-s2.0-85181822086
ER  - 
@article{
author = "Bjørklund, Geir and Buha-Đorđević, Aleksandra and Hamdan, Halla and Wallace, David R. and Peana, Massimiliano",
year = "2024",
abstract = "Autoimmunity is a multifaceted disorder influenced by both genetic and environmental factors, and metal exposure has been implicated as a potential catalyst, especially in autoimmune diseases affecting the central nervous system. Notably, metals like mercury, lead, and aluminum exhibit well-established neurotoxic effects, yet the precise mechanisms by which they elicit autoimmune responses in susceptible individuals remain unclear. Recent studies propose that metal-induced autoimmunity may arise from direct toxic effects on immune cells and tissues, coupled with indirect impacts on the gut microbiome and the blood-brain barrier. These effects can activate self-reactive T cells, prompting the production of autoantibodies, inflammatory responses, and tissue damage. Diagnosing metal-induced autoimmunity proves challenging due to nonspecific symptoms and a lack of reliable biomarkers. Treatment typically involves chelation therapy to eliminate excess metals and immunomodulatory agents to suppress autoimmune responses. Prevention strategies include lifestyle adjustments to reduce metal exposure and avoiding occupational and environmental risks. Prognosis is generally favorable with proper treatment; however, untreated cases may lead to autoimmune disorder progression and irreversible organ damage, particularly in the brain. Future research aims to identify genetic and environmental risk factors, enhance diagnostic precision, and explore novel treatment approaches for improved prevention and management of this intricate and debilitating disease.",
publisher = "Elsevier B.V.",
journal = "Autoimmunity Reviews",
title = "Metal-induced autoimmunity in neurological disorders: A review of current understanding and future directions",
volume = "23",
number = "3",
doi = "10.1016/j.autrev.2023.103509, 2-s2.0-85181822086"
}
Bjørklund, G., Buha-Đorđević, A., Hamdan, H., Wallace, D. R.,& Peana, M.. (2024). Metal-induced autoimmunity in neurological disorders: A review of current understanding and future directions. in Autoimmunity Reviews
Elsevier B.V.., 23(3).
https://doi.org/10.1016/j.autrev.2023.103509
Bjørklund G, Buha-Đorđević A, Hamdan H, Wallace DR, Peana M. Metal-induced autoimmunity in neurological disorders: A review of current understanding and future directions. in Autoimmunity Reviews. 2024;23(3).
doi:10.1016/j.autrev.2023.103509 .
Bjørklund, Geir, Buha-Đorđević, Aleksandra, Hamdan, Halla, Wallace, David R., Peana, Massimiliano, "Metal-induced autoimmunity in neurological disorders: A review of current understanding and future directions" in Autoimmunity Reviews, 23, no. 3 (2024),
https://doi.org/10.1016/j.autrev.2023.103509 . .
5

Acetamiprid's degradation products and mechanism: Part II – Inert atmosphere and charge storage

Popadić, Daliborka; Krstić, Jugoslav; Janošević-Ležaić, Aleksandra; Popović, Maja; Milojević-Rakić, Maja; Ignjatović, Ljubiša; Bajuk-Bogdanović, Danica; Gavrilov, Nemanja

(Elsevier B.V., 2024)

TY  - JOUR
AU  - Popadić, Daliborka
AU  - Krstić, Jugoslav
AU  - Janošević-Ležaić, Aleksandra
AU  - Popović, Maja
AU  - Milojević-Rakić, Maja
AU  - Ignjatović, Ljubiša
AU  - Bajuk-Bogdanović, Danica
AU  - Gavrilov, Nemanja
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5405
AB  - Reuse and/or recycling of spent adsorbents is taking a central role in modern thinking and catalyzed carbonization is the way forward. Herein we explore the carbonization of adsorbed acetamiprid, in an inert atmosphere, as a way of recycling and producing nitrogen-rich carbon material for potential use in supercapacitors. Added value material and the reuse of the adsorbent were achieved by carbonization at 700 °C under argon. The formation of a nitrogen-doped carbon layer as an active material on the adsorbent, bonded through a C-Si linkage, has been conclusively verified through elemental composition quantification using XPS and EDX measurements. Two-stage catalytic decomposition and condensation of the adsorbed pesticide is followed by TGA and TPD-MS. Attained carbon-based materials give stable Faradaic capacitance with a slight dependency on the number of adsorbing cycles. Capacitance calculated with respect to the adlayer carbon material reaches values as high as 610 F g−1. Galvanostatic Charge/Discharge measurement confirmed the stability of explored materials with a slight increase in capacitance over 1000 cycles. The presented results envisage electroactive materials preparation from environmental pollutants, adding value to spent adsorbents.
PB  - Elsevier B.V.
T2  - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
T1  - Acetamiprid's degradation products and mechanism: Part II – Inert atmosphere and charge storage
VL  - 308
DO  - 10.1016/j.saa.2023.123772
ER  - 
@article{
author = "Popadić, Daliborka and Krstić, Jugoslav and Janošević-Ležaić, Aleksandra and Popović, Maja and Milojević-Rakić, Maja and Ignjatović, Ljubiša and Bajuk-Bogdanović, Danica and Gavrilov, Nemanja",
year = "2024",
abstract = "Reuse and/or recycling of spent adsorbents is taking a central role in modern thinking and catalyzed carbonization is the way forward. Herein we explore the carbonization of adsorbed acetamiprid, in an inert atmosphere, as a way of recycling and producing nitrogen-rich carbon material for potential use in supercapacitors. Added value material and the reuse of the adsorbent were achieved by carbonization at 700 °C under argon. The formation of a nitrogen-doped carbon layer as an active material on the adsorbent, bonded through a C-Si linkage, has been conclusively verified through elemental composition quantification using XPS and EDX measurements. Two-stage catalytic decomposition and condensation of the adsorbed pesticide is followed by TGA and TPD-MS. Attained carbon-based materials give stable Faradaic capacitance with a slight dependency on the number of adsorbing cycles. Capacitance calculated with respect to the adlayer carbon material reaches values as high as 610 F g−1. Galvanostatic Charge/Discharge measurement confirmed the stability of explored materials with a slight increase in capacitance over 1000 cycles. The presented results envisage electroactive materials preparation from environmental pollutants, adding value to spent adsorbents.",
publisher = "Elsevier B.V.",
journal = "Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy",
title = "Acetamiprid's degradation products and mechanism: Part II – Inert atmosphere and charge storage",
volume = "308",
doi = "10.1016/j.saa.2023.123772"
}
Popadić, D., Krstić, J., Janošević-Ležaić, A., Popović, M., Milojević-Rakić, M., Ignjatović, L., Bajuk-Bogdanović, D.,& Gavrilov, N.. (2024). Acetamiprid's degradation products and mechanism: Part II – Inert atmosphere and charge storage. in Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
Elsevier B.V.., 308.
https://doi.org/10.1016/j.saa.2023.123772
Popadić D, Krstić J, Janošević-Ležaić A, Popović M, Milojević-Rakić M, Ignjatović L, Bajuk-Bogdanović D, Gavrilov N. Acetamiprid's degradation products and mechanism: Part II – Inert atmosphere and charge storage. in Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy. 2024;308.
doi:10.1016/j.saa.2023.123772 .
Popadić, Daliborka, Krstić, Jugoslav, Janošević-Ležaić, Aleksandra, Popović, Maja, Milojević-Rakić, Maja, Ignjatović, Ljubiša, Bajuk-Bogdanović, Danica, Gavrilov, Nemanja, "Acetamiprid's degradation products and mechanism: Part II – Inert atmosphere and charge storage" in Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, 308 (2024),
https://doi.org/10.1016/j.saa.2023.123772 . .

The Comparative Analysis of the Impact of N-Acetyl-L-Cysteine and its Combination with Propolis on Quality-of-Life in Patients with Acute Bronchitis

Tadić, Ivana; Žuža, Olivera; Žujović, Dejan; Agić, Anita; Korčok, Davor; Lazović, Biljana; Stevanović, Dejan; Đorđević, Brižita

(Association of Pharmaceutical Teachers of India, 2024)

TY  - JOUR
AU  - Tadić, Ivana
AU  - Žuža, Olivera
AU  - Žujović, Dejan
AU  - Agić, Anita
AU  - Korčok, Davor
AU  - Lazović, Biljana
AU  - Stevanović, Dejan
AU  - Đorđević, Brižita
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5408
AB  - Objectives: Evidence suggests that both N-Acetyl-L-cysteine (NAC) and NAC in combination with propolis (NAC-P) reduce symptoms of acute bronchitis and improve Health-Related Quality of Life (HRQoL). This study aimed to compare the impact of NAC and NAC-P therapy on acute bronchitis patients' quality of life. Design, setting, and subjects: A randomized, single-blind parallel-group study was achieved at Municipal Institute for Lung Diseases and Tuberculosis Medical Centre, Belgrade, Serbia. Patients with acute bronchitis were randomly assigned into two groups to receive NAC (200mg three times a day) or NAC-P (200mg+80mg three times a day) orally for a minimum of ten days. HRQoL of patients was measured twice (at two appointments) using the Leicester Cough Questionnaire (LCQ) and EQ-5D questionnaires. Results: The study included 42 patients in the NAC-P group and 43 in the NAC group. The correlation between the LCQ total score and Health State Values (HSV) was positive and statistically significant in the NAC-P and the NAC groups at the second appointment. The differences between values of EQ-5D scores were slightly higher (but not statistically significant) in the NAC-P group compared to the NAC group, indicating a more remarkable improvement of HRQoL using NAC-P. Conclusion: NAC-P and NAC may improve HRQoL in patients with acute bronchitis. EQ-5D scale values indicated that NAC-P might improve quality of life more than NAC. Our results may provide the basis for better decision-making by healthcare professionals when choosing the right therapy.
PB  - Association of Pharmaceutical Teachers of India
T2  - Indian Journal of Pharmaceutical Education and Research
T1  - The Comparative Analysis of the Impact of N-Acetyl-L-Cysteine and its Combination with Propolis on Quality-of-Life in Patients with Acute Bronchitis
VL  - 58
IS  - 1
SP  - 348
EP  - 354
DO  - 10.5530/ijper.58.1.38
ER  - 
@article{
author = "Tadić, Ivana and Žuža, Olivera and Žujović, Dejan and Agić, Anita and Korčok, Davor and Lazović, Biljana and Stevanović, Dejan and Đorđević, Brižita",
year = "2024",
abstract = "Objectives: Evidence suggests that both N-Acetyl-L-cysteine (NAC) and NAC in combination with propolis (NAC-P) reduce symptoms of acute bronchitis and improve Health-Related Quality of Life (HRQoL). This study aimed to compare the impact of NAC and NAC-P therapy on acute bronchitis patients' quality of life. Design, setting, and subjects: A randomized, single-blind parallel-group study was achieved at Municipal Institute for Lung Diseases and Tuberculosis Medical Centre, Belgrade, Serbia. Patients with acute bronchitis were randomly assigned into two groups to receive NAC (200mg three times a day) or NAC-P (200mg+80mg three times a day) orally for a minimum of ten days. HRQoL of patients was measured twice (at two appointments) using the Leicester Cough Questionnaire (LCQ) and EQ-5D questionnaires. Results: The study included 42 patients in the NAC-P group and 43 in the NAC group. The correlation between the LCQ total score and Health State Values (HSV) was positive and statistically significant in the NAC-P and the NAC groups at the second appointment. The differences between values of EQ-5D scores were slightly higher (but not statistically significant) in the NAC-P group compared to the NAC group, indicating a more remarkable improvement of HRQoL using NAC-P. Conclusion: NAC-P and NAC may improve HRQoL in patients with acute bronchitis. EQ-5D scale values indicated that NAC-P might improve quality of life more than NAC. Our results may provide the basis for better decision-making by healthcare professionals when choosing the right therapy.",
publisher = "Association of Pharmaceutical Teachers of India",
journal = "Indian Journal of Pharmaceutical Education and Research",
title = "The Comparative Analysis of the Impact of N-Acetyl-L-Cysteine and its Combination with Propolis on Quality-of-Life in Patients with Acute Bronchitis",
volume = "58",
number = "1",
pages = "348-354",
doi = "10.5530/ijper.58.1.38"
}
Tadić, I., Žuža, O., Žujović, D., Agić, A., Korčok, D., Lazović, B., Stevanović, D.,& Đorđević, B.. (2024). The Comparative Analysis of the Impact of N-Acetyl-L-Cysteine and its Combination with Propolis on Quality-of-Life in Patients with Acute Bronchitis. in Indian Journal of Pharmaceutical Education and Research
Association of Pharmaceutical Teachers of India., 58(1), 348-354.
https://doi.org/10.5530/ijper.58.1.38
Tadić I, Žuža O, Žujović D, Agić A, Korčok D, Lazović B, Stevanović D, Đorđević B. The Comparative Analysis of the Impact of N-Acetyl-L-Cysteine and its Combination with Propolis on Quality-of-Life in Patients with Acute Bronchitis. in Indian Journal of Pharmaceutical Education and Research. 2024;58(1):348-354.
doi:10.5530/ijper.58.1.38 .
Tadić, Ivana, Žuža, Olivera, Žujović, Dejan, Agić, Anita, Korčok, Davor, Lazović, Biljana, Stevanović, Dejan, Đorđević, Brižita, "The Comparative Analysis of the Impact of N-Acetyl-L-Cysteine and its Combination with Propolis on Quality-of-Life in Patients with Acute Bronchitis" in Indian Journal of Pharmaceutical Education and Research, 58, no. 1 (2024):348-354,
https://doi.org/10.5530/ijper.58.1.38 . .

Hepato-renal toxicity of low dose metal(oid)s mixture in real-life risk simulation in rats: Effects on Nrf2/HO-1 signalling and redox status

Vukelić, Dragana; Baralić, Katarina; Marić, Đurđica; Đukić-Ćosić, Danijela; Bulat, Zorica; Panieri, Emiliano; Saso, Luciano; Buha-Đorđević, Aleksandra

(Elsevier B.V., 2024)

TY  - JOUR
AU  - Vukelić, Dragana
AU  - Baralić, Katarina
AU  - Marić, Đurđica
AU  - Đukić-Ćosić, Danijela
AU  - Bulat, Zorica
AU  - Panieri, Emiliano
AU  - Saso, Luciano
AU  - Buha-Đorđević, Aleksandra
PY  - 2024
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5313
AB  - The understanding that humans are exposed to a low level of toxic metals and metalloids in their lifetime has resulted in a shift in scientific and regulatory perspectives from the traditional evaluation of single metal toxicity to complex mixtures, relevant to real-life exposure. Therefore, the aim of this study was to examine the impact of real-life, 90-days exposure to mixture of toxic metal(oid)s, Cd, Pb, Ni, Cr, As and Hg, on the nuclear factor erythroid 2–related factor 2 and hemoxygenase-1 (Nrf2/HO-1) signalling and redox status by assessing total sulfhydryl groups (SH), glutathione (GSH), superoxide dismutase activity (SOD), malondialdehyde (MDA), and ischemia modified albumin (IMA) in the liver and kidney of Wistar rats. Animals (20 males and 20 females) were randomized in 2 control and 6 treated groups that received by oral gavage mixture of metal(oid)s solutions in doses that reflect blood metal(oid) levels determined in previous human biomonitoring study as benchmark dose (F/M _BMD), median (F/M _MED), and 95th percentile (F/M _95). Our results have shown that metal(oid)s mixture impaired the activation of the Nrf2/HO-1 pathway in the kidney and liver of male rats and kidney of female rats, followed by depletion of GSH levels in males. Additionally, in males elevated levels of IMA in the liver were observed, while in both genders increased MDA levels were observed in the kidney. Interestingly, the effects were more pronounced in male than in female rats. This study is among the first that examined hepato-renal toxic mechanisms of real-life metal mixture exposure, while our results might be of immense importance for assessing the risk of exposure to mixtures of toxic substances.
PB  - Elsevier B.V.
T2  - Science of the Total Environment
T1  - Hepato-renal toxicity of low dose metal(oid)s mixture in real-life risk simulation in rats: Effects on Nrf2/HO-1 signalling and redox status
VL  - 908
DO  - 10.1016/j.scitotenv.2023.168352
ER  - 
@article{
author = "Vukelić, Dragana and Baralić, Katarina and Marić, Đurđica and Đukić-Ćosić, Danijela and Bulat, Zorica and Panieri, Emiliano and Saso, Luciano and Buha-Đorđević, Aleksandra",
year = "2024",
abstract = "The understanding that humans are exposed to a low level of toxic metals and metalloids in their lifetime has resulted in a shift in scientific and regulatory perspectives from the traditional evaluation of single metal toxicity to complex mixtures, relevant to real-life exposure. Therefore, the aim of this study was to examine the impact of real-life, 90-days exposure to mixture of toxic metal(oid)s, Cd, Pb, Ni, Cr, As and Hg, on the nuclear factor erythroid 2–related factor 2 and hemoxygenase-1 (Nrf2/HO-1) signalling and redox status by assessing total sulfhydryl groups (SH), glutathione (GSH), superoxide dismutase activity (SOD), malondialdehyde (MDA), and ischemia modified albumin (IMA) in the liver and kidney of Wistar rats. Animals (20 males and 20 females) were randomized in 2 control and 6 treated groups that received by oral gavage mixture of metal(oid)s solutions in doses that reflect blood metal(oid) levels determined in previous human biomonitoring study as benchmark dose (F/M _BMD), median (F/M _MED), and 95th percentile (F/M _95). Our results have shown that metal(oid)s mixture impaired the activation of the Nrf2/HO-1 pathway in the kidney and liver of male rats and kidney of female rats, followed by depletion of GSH levels in males. Additionally, in males elevated levels of IMA in the liver were observed, while in both genders increased MDA levels were observed in the kidney. Interestingly, the effects were more pronounced in male than in female rats. This study is among the first that examined hepato-renal toxic mechanisms of real-life metal mixture exposure, while our results might be of immense importance for assessing the risk of exposure to mixtures of toxic substances.",
publisher = "Elsevier B.V.",
journal = "Science of the Total Environment",
title = "Hepato-renal toxicity of low dose metal(oid)s mixture in real-life risk simulation in rats: Effects on Nrf2/HO-1 signalling and redox status",
volume = "908",
doi = "10.1016/j.scitotenv.2023.168352"
}
Vukelić, D., Baralić, K., Marić, Đ., Đukić-Ćosić, D., Bulat, Z., Panieri, E., Saso, L.,& Buha-Đorđević, A.. (2024). Hepato-renal toxicity of low dose metal(oid)s mixture in real-life risk simulation in rats: Effects on Nrf2/HO-1 signalling and redox status. in Science of the Total Environment
Elsevier B.V.., 908.
https://doi.org/10.1016/j.scitotenv.2023.168352
Vukelić D, Baralić K, Marić Đ, Đukić-Ćosić D, Bulat Z, Panieri E, Saso L, Buha-Đorđević A. Hepato-renal toxicity of low dose metal(oid)s mixture in real-life risk simulation in rats: Effects on Nrf2/HO-1 signalling and redox status. in Science of the Total Environment. 2024;908.
doi:10.1016/j.scitotenv.2023.168352 .
Vukelić, Dragana, Baralić, Katarina, Marić, Đurđica, Đukić-Ćosić, Danijela, Bulat, Zorica, Panieri, Emiliano, Saso, Luciano, Buha-Đorđević, Aleksandra, "Hepato-renal toxicity of low dose metal(oid)s mixture in real-life risk simulation in rats: Effects on Nrf2/HO-1 signalling and redox status" in Science of the Total Environment, 908 (2024),
https://doi.org/10.1016/j.scitotenv.2023.168352 . .

Phenolics and Flavonoid Content in Selected Seeds from the Serbian Market

Dodevska, Margarita; Ivanović, Nevena; Kukić-Marković, Jelena; Jovanović, Verica

(MDPI, 2023)

TY  - CONF
AU  - Dodevska, Margarita
AU  - Ivanović, Nevena
AU  - Kukić-Marković, Jelena
AU  - Jovanović, Verica
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5547
AB  - Objectives: Edible seeds are usually consumed as common food ingredients. They are
considered to have a rich nutrient profile, containing different macro and micronutrients, as well
as some biologically active compounds with positive health effects, such as different phenolics.
The aim of this work was to determine total phenolics (TPC) and total flavonoid content (TFC)
in selected commercial seeds samples from the Serbian market. Methods: Samples of nine seeds
were investigated (sesame and black sesame, raw and roasted sunflower, raw and roasted pumpkin,
hemp, chia and linseed). The samples of native seeds and those defatted using dichloromethane
(maceration and Soxhlet extraction) were extracted with 80% methanol. Obtained hydro-methanol
extracts were dried and further analysed using spectrophotometric methods: TPC was determined
using Folin–Ciocalteu (FC) reagent and expressed as gallic acid equivalents (GAE), while TFC was
measured based on the reaction between flavonoids and aluminium chloride and expressed as
catechin equivalents (KE). Results: In general, hydro-methanol extracts of seed samples defatted
using Soxhlet extraction had the highest TPC and TFC contents. TPC values ranged from 9.47 g
GAE/mg (raw pumpkin seed) to over 170 g GAE/mg (raw sunflower seeds). As for TFC, the
highest amount was measured in extracts of defatted raw sunflower seeds (over 150 g KE/mg),
while roasted pumpkin and hemp seeds’ extracts were practically devoid of flavonoids. Conclusion:
Our results confirmed the fact that certain defatted seeds, which are usually considered as waste
products in oil production, could be considered as valuable sources of certain secondary plant
metabolites, implicating further investigations on their composition and potential in the development
of functional foods.
PB  - MDPI
C3  - Proceedings, The 14th European Nutrition Conference FENS, Serbia, Belgrade, 14–17 November
T1  - Phenolics and Flavonoid Content in Selected Seeds from the Serbian Market
VL  - 91
IS  - 1
SP  - 374
EP  - 374
DO  - 10.3390/proceedings2023091374
ER  - 
@conference{
author = "Dodevska, Margarita and Ivanović, Nevena and Kukić-Marković, Jelena and Jovanović, Verica",
year = "2023",
abstract = "Objectives: Edible seeds are usually consumed as common food ingredients. They are
considered to have a rich nutrient profile, containing different macro and micronutrients, as well
as some biologically active compounds with positive health effects, such as different phenolics.
The aim of this work was to determine total phenolics (TPC) and total flavonoid content (TFC)
in selected commercial seeds samples from the Serbian market. Methods: Samples of nine seeds
were investigated (sesame and black sesame, raw and roasted sunflower, raw and roasted pumpkin,
hemp, chia and linseed). The samples of native seeds and those defatted using dichloromethane
(maceration and Soxhlet extraction) were extracted with 80% methanol. Obtained hydro-methanol
extracts were dried and further analysed using spectrophotometric methods: TPC was determined
using Folin–Ciocalteu (FC) reagent and expressed as gallic acid equivalents (GAE), while TFC was
measured based on the reaction between flavonoids and aluminium chloride and expressed as
catechin equivalents (KE). Results: In general, hydro-methanol extracts of seed samples defatted
using Soxhlet extraction had the highest TPC and TFC contents. TPC values ranged from 9.47 g
GAE/mg (raw pumpkin seed) to over 170 g GAE/mg (raw sunflower seeds). As for TFC, the
highest amount was measured in extracts of defatted raw sunflower seeds (over 150 g KE/mg),
while roasted pumpkin and hemp seeds’ extracts were practically devoid of flavonoids. Conclusion:
Our results confirmed the fact that certain defatted seeds, which are usually considered as waste
products in oil production, could be considered as valuable sources of certain secondary plant
metabolites, implicating further investigations on their composition and potential in the development
of functional foods.",
publisher = "MDPI",
journal = "Proceedings, The 14th European Nutrition Conference FENS, Serbia, Belgrade, 14–17 November",
title = "Phenolics and Flavonoid Content in Selected Seeds from the Serbian Market",
volume = "91",
number = "1",
pages = "374-374",
doi = "10.3390/proceedings2023091374"
}
Dodevska, M., Ivanović, N., Kukić-Marković, J.,& Jovanović, V.. (2023). Phenolics and Flavonoid Content in Selected Seeds from the Serbian Market. in Proceedings, The 14th European Nutrition Conference FENS, Serbia, Belgrade, 14–17 November
MDPI., 91(1), 374-374.
https://doi.org/10.3390/proceedings2023091374
Dodevska M, Ivanović N, Kukić-Marković J, Jovanović V. Phenolics and Flavonoid Content in Selected Seeds from the Serbian Market. in Proceedings, The 14th European Nutrition Conference FENS, Serbia, Belgrade, 14–17 November. 2023;91(1):374-374.
doi:10.3390/proceedings2023091374 .
Dodevska, Margarita, Ivanović, Nevena, Kukić-Marković, Jelena, Jovanović, Verica, "Phenolics and Flavonoid Content in Selected Seeds from the Serbian Market" in Proceedings, The 14th European Nutrition Conference FENS, Serbia, Belgrade, 14–17 November, 91, no. 1 (2023):374-374,
https://doi.org/10.3390/proceedings2023091374 . .

Dietary Intakes of Sugar in the Student Population from Fruits and Beverages

Dodevska, Margarita; Kukić-Marković, Jelena; Ivanović, Nevena; Perišić, Tamara; Jovanović, Verica

(MDPI, 2023)

TY  - CONF
AU  - Dodevska, Margarita
AU  - Kukić-Marković, Jelena
AU  - Ivanović, Nevena
AU  - Perišić, Tamara
AU  - Jovanović, Verica
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5546
AB  - Objectives: A healthy diet includes the intake of sugar (up to 5%) from natural sources,
because added sugars increase energy but not nutritional value, and are associated with an increased
risk of developing obesity, cardiovascular diseases and hypertension. Sugars present in fresh fruit,
fruit juices and nectars, refreshing soft drinks and alcoholic drinks usually represent half of the
total sugar intake. The aim of this study was to analyze, through surveys, the habits of the student
population regarding the consumption of food from the sources mentioned and to determine the
amount of sugar consumed. Methods: 123 students (male: 36, female: 87), aged 19 to 32, participated
in this research. Data collection was voluntary and anonymous, and the survey was conducted
using an electronic questionnaire during February 2023. The data on the sugar content of the foods
mentioned were taken from the declarations of the products that are available for free sale. Results:
Based on the processed results from the completed questionnaires, it was concluded that from the
sources mentioned, sugar is represented by 3.7% in relation to the total energy intake, and added
sugar by 2.4% if 2000 kcal/day is consumed. In relation to the representation of carbohydrates of
60% in relation to the total energy intake, the sugar content from the tested sources accounted for
6.3%, and added sugar accounted for 4.0%. Students reported that they most often do not consume
hard liquor (88% of respondents), and that they eat a teaspoon of honey/sugar once a day (14% of
respondents). Conclusion: Although the obtained results are very acceptable and encouraging, it
is necessary to act additionally in terms of increasing awareness of the reduced intake of products
with added sugars and their replacement with those that contain biologically active compounds in
addition to natural sugar composition.
PB  - MDPI
C3  - Proceedings, The 14th European Nutrition Conference FENS, Serbia, Belgrade, 14–17 November
T1  - Dietary Intakes of Sugar in the Student Population from Fruits and Beverages
VL  - 91
IS  - 1
SP  - 215
EP  - 215
DO  - 10.3390/proceedings2023091215
ER  - 
@conference{
author = "Dodevska, Margarita and Kukić-Marković, Jelena and Ivanović, Nevena and Perišić, Tamara and Jovanović, Verica",
year = "2023",
abstract = "Objectives: A healthy diet includes the intake of sugar (up to 5%) from natural sources,
because added sugars increase energy but not nutritional value, and are associated with an increased
risk of developing obesity, cardiovascular diseases and hypertension. Sugars present in fresh fruit,
fruit juices and nectars, refreshing soft drinks and alcoholic drinks usually represent half of the
total sugar intake. The aim of this study was to analyze, through surveys, the habits of the student
population regarding the consumption of food from the sources mentioned and to determine the
amount of sugar consumed. Methods: 123 students (male: 36, female: 87), aged 19 to 32, participated
in this research. Data collection was voluntary and anonymous, and the survey was conducted
using an electronic questionnaire during February 2023. The data on the sugar content of the foods
mentioned were taken from the declarations of the products that are available for free sale. Results:
Based on the processed results from the completed questionnaires, it was concluded that from the
sources mentioned, sugar is represented by 3.7% in relation to the total energy intake, and added
sugar by 2.4% if 2000 kcal/day is consumed. In relation to the representation of carbohydrates of
60% in relation to the total energy intake, the sugar content from the tested sources accounted for
6.3%, and added sugar accounted for 4.0%. Students reported that they most often do not consume
hard liquor (88% of respondents), and that they eat a teaspoon of honey/sugar once a day (14% of
respondents). Conclusion: Although the obtained results are very acceptable and encouraging, it
is necessary to act additionally in terms of increasing awareness of the reduced intake of products
with added sugars and their replacement with those that contain biologically active compounds in
addition to natural sugar composition.",
publisher = "MDPI",
journal = "Proceedings, The 14th European Nutrition Conference FENS, Serbia, Belgrade, 14–17 November",
title = "Dietary Intakes of Sugar in the Student Population from Fruits and Beverages",
volume = "91",
number = "1",
pages = "215-215",
doi = "10.3390/proceedings2023091215"
}
Dodevska, M., Kukić-Marković, J., Ivanović, N., Perišić, T.,& Jovanović, V.. (2023). Dietary Intakes of Sugar in the Student Population from Fruits and Beverages. in Proceedings, The 14th European Nutrition Conference FENS, Serbia, Belgrade, 14–17 November
MDPI., 91(1), 215-215.
https://doi.org/10.3390/proceedings2023091215
Dodevska M, Kukić-Marković J, Ivanović N, Perišić T, Jovanović V. Dietary Intakes of Sugar in the Student Population from Fruits and Beverages. in Proceedings, The 14th European Nutrition Conference FENS, Serbia, Belgrade, 14–17 November. 2023;91(1):215-215.
doi:10.3390/proceedings2023091215 .
Dodevska, Margarita, Kukić-Marković, Jelena, Ivanović, Nevena, Perišić, Tamara, Jovanović, Verica, "Dietary Intakes of Sugar in the Student Population from Fruits and Beverages" in Proceedings, The 14th European Nutrition Conference FENS, Serbia, Belgrade, 14–17 November, 91, no. 1 (2023):215-215,
https://doi.org/10.3390/proceedings2023091215 . .

Knowledge, attitudes and dietary practices of health proffesionals regarding sustainable diet

Ivanović, Nevena; Zeković, Milica; Kukić-Marković, Jelena; Dodevska, Margarita; Đorđević, Brižita

(International Conference of Raw Materials to Processed Foods, 2023)

TY  - CONF
AU  - Ivanović, Nevena
AU  - Zeković, Milica
AU  - Kukić-Marković, Jelena
AU  - Dodevska, Margarita
AU  - Đorđević, Brižita
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5515
AB  - Sustainable diets are defined by the Food and Agriculture Organisation of the United Nations (FAO) as “diets with low environmental impact that contribute to food and nutrition security and healthy lives for present and future generations”. ...
PB  - International Conference of Raw Materials to Processed Foods
C3  - 3rd International Conference on Raw Materials to Processed Foods, Istanbul, Turkey, 18-19 May 2023, Procedings Book
T1  - Knowledge, attitudes and dietary practices of health proffesionals regarding sustainable diet
SP  - 115
EP  - 115
UR  - https://hdl.handle.net/21.15107/rcub_farfar_5515
ER  - 
@conference{
author = "Ivanović, Nevena and Zeković, Milica and Kukić-Marković, Jelena and Dodevska, Margarita and Đorđević, Brižita",
year = "2023",
abstract = "Sustainable diets are defined by the Food and Agriculture Organisation of the United Nations (FAO) as “diets with low environmental impact that contribute to food and nutrition security and healthy lives for present and future generations”. ...",
publisher = "International Conference of Raw Materials to Processed Foods",
journal = "3rd International Conference on Raw Materials to Processed Foods, Istanbul, Turkey, 18-19 May 2023, Procedings Book",
title = "Knowledge, attitudes and dietary practices of health proffesionals regarding sustainable diet",
pages = "115-115",
url = "https://hdl.handle.net/21.15107/rcub_farfar_5515"
}
Ivanović, N., Zeković, M., Kukić-Marković, J., Dodevska, M.,& Đorđević, B.. (2023). Knowledge, attitudes and dietary practices of health proffesionals regarding sustainable diet. in 3rd International Conference on Raw Materials to Processed Foods, Istanbul, Turkey, 18-19 May 2023, Procedings Book
International Conference of Raw Materials to Processed Foods., 115-115.
https://hdl.handle.net/21.15107/rcub_farfar_5515
Ivanović N, Zeković M, Kukić-Marković J, Dodevska M, Đorđević B. Knowledge, attitudes and dietary practices of health proffesionals regarding sustainable diet. in 3rd International Conference on Raw Materials to Processed Foods, Istanbul, Turkey, 18-19 May 2023, Procedings Book. 2023;:115-115.
https://hdl.handle.net/21.15107/rcub_farfar_5515 .
Ivanović, Nevena, Zeković, Milica, Kukić-Marković, Jelena, Dodevska, Margarita, Đorđević, Brižita, "Knowledge, attitudes and dietary practices of health proffesionals regarding sustainable diet" in 3rd International Conference on Raw Materials to Processed Foods, Istanbul, Turkey, 18-19 May 2023, Procedings Book (2023):115-115,
https://hdl.handle.net/21.15107/rcub_farfar_5515 .

The supplementation of a high dose of fish oil during pregnancy and lactation led to an elevation in Mfsd2a expression without any changes in docosahexaenoic acid levels in the retina of healthy 2-month-old mouse offspring

Jovanović-Macura, Irena; Đuričić, Ivana; Major, Tamara; Milanović, Desanka; Šobajić, Slađana; Kanazir, Selma; Ivković, Sanja

(Frontiers Media SA, 2023)

TY  - JOUR
AU  - Jovanović-Macura, Irena
AU  - Đuričić, Ivana
AU  - Major, Tamara
AU  - Milanović, Desanka
AU  - Šobajić, Slađana
AU  - Kanazir, Selma
AU  - Ivković, Sanja
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5543
AB  - Introduction: During fetal development, the proper development of neural and visual systems relies on the maternal supplementation of omega-3 fatty acids through placental transfer. Pregnant women are strongly advised to augment their diet with additional sources of omega-3, such as fish oil (FO). This supplementation has been linked to a reduced risk of preterm birth, pre-eclampsia, and perinatal depression. Recently, higher doses of omega-3 supplementation have been recommended for pregnant women. Considering that omega-3 fatty acids, particularly docosahexaenoic acid (DHA), play a crucial role in maintaining the delicate homeostasis required for the proper functioning of the retina and photoreceptors the effects of high-dose fish oil (FO) supplementation during pregnancy and lactation on the retina and retinal pigmented epithelium (RPE) in healthy offspring warrant better understanding. Methods: The fatty acid content and the changes in the expression of the genes regulating cholesterol homeostasis and DHA transport in the retina and RPE were evaluated following the high-dose FO supplementation. Results: Our study demonstrated that despite the high-dose FO treatment during pregnancy and lactation, the rigorous DHA homeostasis in the retina and RPE of the two-month-old offspring remained balanced. Another significant finding of this study is the increase in the expression levels of major facilitator superfamily domain-containing protein (Mfsd2a), a primary DHA transporter. Mfsd2a also serves as a major regulator of transcytosis during development, and a reduction in Mfsd2a levels poses a major risk for the development of leaky blood vessels. Conclusion: Impairment of the blood-retinal barrier (BRB) is associated with the development of numerous ocular diseases, and a better understanding of how to manipulate transcytosis in the BRB during development can enhance drug delivery through the BRB or contribute to the repair of central nervous system (CNS) barriers.
PB  - Frontiers Media SA
T2  - Frontiers in Nutrition
T1  - The supplementation of a high dose of fish oil during pregnancy and lactation led to an elevation in Mfsd2a expression without any changes in docosahexaenoic acid levels in the retina of healthy 2-month-old mouse offspring
VL  - 10
SP  - 1330414
DO  - 10.3389/fnut.2023.1330414
ER  - 
@article{
author = "Jovanović-Macura, Irena and Đuričić, Ivana and Major, Tamara and Milanović, Desanka and Šobajić, Slađana and Kanazir, Selma and Ivković, Sanja",
year = "2023",
abstract = "Introduction: During fetal development, the proper development of neural and visual systems relies on the maternal supplementation of omega-3 fatty acids through placental transfer. Pregnant women are strongly advised to augment their diet with additional sources of omega-3, such as fish oil (FO). This supplementation has been linked to a reduced risk of preterm birth, pre-eclampsia, and perinatal depression. Recently, higher doses of omega-3 supplementation have been recommended for pregnant women. Considering that omega-3 fatty acids, particularly docosahexaenoic acid (DHA), play a crucial role in maintaining the delicate homeostasis required for the proper functioning of the retina and photoreceptors the effects of high-dose fish oil (FO) supplementation during pregnancy and lactation on the retina and retinal pigmented epithelium (RPE) in healthy offspring warrant better understanding. Methods: The fatty acid content and the changes in the expression of the genes regulating cholesterol homeostasis and DHA transport in the retina and RPE were evaluated following the high-dose FO supplementation. Results: Our study demonstrated that despite the high-dose FO treatment during pregnancy and lactation, the rigorous DHA homeostasis in the retina and RPE of the two-month-old offspring remained balanced. Another significant finding of this study is the increase in the expression levels of major facilitator superfamily domain-containing protein (Mfsd2a), a primary DHA transporter. Mfsd2a also serves as a major regulator of transcytosis during development, and a reduction in Mfsd2a levels poses a major risk for the development of leaky blood vessels. Conclusion: Impairment of the blood-retinal barrier (BRB) is associated with the development of numerous ocular diseases, and a better understanding of how to manipulate transcytosis in the BRB during development can enhance drug delivery through the BRB or contribute to the repair of central nervous system (CNS) barriers.",
publisher = "Frontiers Media SA",
journal = "Frontiers in Nutrition",
title = "The supplementation of a high dose of fish oil during pregnancy and lactation led to an elevation in Mfsd2a expression without any changes in docosahexaenoic acid levels in the retina of healthy 2-month-old mouse offspring",
volume = "10",
pages = "1330414",
doi = "10.3389/fnut.2023.1330414"
}
Jovanović-Macura, I., Đuričić, I., Major, T., Milanović, D., Šobajić, S., Kanazir, S.,& Ivković, S.. (2023). The supplementation of a high dose of fish oil during pregnancy and lactation led to an elevation in Mfsd2a expression without any changes in docosahexaenoic acid levels in the retina of healthy 2-month-old mouse offspring. in Frontiers in Nutrition
Frontiers Media SA., 10, 1330414.
https://doi.org/10.3389/fnut.2023.1330414
Jovanović-Macura I, Đuričić I, Major T, Milanović D, Šobajić S, Kanazir S, Ivković S. The supplementation of a high dose of fish oil during pregnancy and lactation led to an elevation in Mfsd2a expression without any changes in docosahexaenoic acid levels in the retina of healthy 2-month-old mouse offspring. in Frontiers in Nutrition. 2023;10:1330414.
doi:10.3389/fnut.2023.1330414 .
Jovanović-Macura, Irena, Đuričić, Ivana, Major, Tamara, Milanović, Desanka, Šobajić, Slađana, Kanazir, Selma, Ivković, Sanja, "The supplementation of a high dose of fish oil during pregnancy and lactation led to an elevation in Mfsd2a expression without any changes in docosahexaenoic acid levels in the retina of healthy 2-month-old mouse offspring" in Frontiers in Nutrition, 10 (2023):1330414,
https://doi.org/10.3389/fnut.2023.1330414 . .

Characterization of tea aqueous extracts and their utilization for dyeing and functionalizing fabrics of different chemical compositions

Pavun, Leposava; Spasojević, Danijela; Ivanovska, Aleksandra; Lađarević, Jelena; Milenković, Marina; Uskoković-Marković, Snežana

(Macedonian Journal of Chemistry and Chemical Engineering, 2023)

TY  - JOUR
AU  - Pavun, Leposava
AU  - Spasojević, Danijela
AU  - Ivanovska, Aleksandra
AU  - Lađarević, Jelena
AU  - Milenković, Marina
AU  - Uskoković-Marković, Snežana
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5420
AB  - Green, black, rooibos, and hibiscus tea (GT, BT, RT, and HT) aqueous extracts were prepared and
characterized in terms of total flavonoids (TFC) and total phenolic (TPC) contents and antioxidant and
antimicrobial activities. BT has the highest, while HT has the lowest TFC (1213 vs. 415 mg l–1
), while the
extracts’ TPCs (2283 – 7251 mg l–1
) decreased in the following order: BT > GT > RT > HT. Their antioxidant activities of 78.1 – 93.1% and 97.8 – 100% were determined according to DPPH and ABTS methods, respectively. BT and especially GT aqueous extracts possessed mild effects against several microorganisms. All examined extracts have an affinity for dyeing wool, cellulose acetate, polyamide, and cotton,
which is proven by the color strength values of 1.65–19.12. Wool, polyacrylonitrile, polyester, polyamide, cotton, and cellulose acetate functionalized with GT aqueous extract inhibited the growth of S. aureus
and E. coli, while polyacrylonitrile and cotton also inhibited the growth of E. faecalis, and C. albicans,
respectively. Wide inhibition zones for S. aureus were observed for fabrics functionalized with BT aqueous extract. Generally, the investigated fabrics showed very high (81.60 – 100%) ABTS radical scavenging ability independent of the extract used. TPCs have good linear correlations with the antioxidant activities of wool and polyacrylonitrile determined by the DPPH method. Fabrics with different chemical compositions dyed and/or functionalized with GT or BT aqueous extracts can be used to produce high-valueadded medical textiles with therapeutic, prophylactic, and protective functions. They can find potential applications in wound treatment, especially in skin wounds that are susceptible to infection with S. aureus.
Moreover, wool and cotton functionalized with GT or BT aqueous extract can also be considered for use in
disposable medical textiles like bandages and gauze used in the wound-healing process.
Keywords: tea aqueous extract; fabric; functionalization; dyeing; antioxidant and antimicrobial
AB  - Водните екстракти подготвени од црн, зелен, ројбуш и хибискус чај (GT, BT, RT и HT) се
карактеризирани од аспект на количество вкупни флавоноиди (TFC) и вкупни феноли (TPC), и
антиоксидациска и антимикробна активност. BT покажува најголема, а HT најмала TFC (1213 vs.
415 mg l–1), додека TPC во воденте екстракти (2283 – 7251 mg l–1) се намалува според следниов
редослед: BT > GT > RT > HT. Нивната антиоксидациска активност, одредена користејќи ги
методите DPPH и ABTS, се движи соодветно во опсег 78,1 – 93.1 % и 97,8 – 100 %. Водните
екстракти на BT и GT покажаа благ ефект врз одредени микроорганизми. Сите испитувани
екстракти имаат афинитет за боење волна, целулозен ацетат, полиамид и памук, што е потврдено
со одредување на јачина на бојата на ткаенините која изнесува 1,65 – 19.12. Волната,
полиакрилонитрилот, полиестерот, полиамидот, памукот и целулозниот ацетат функционализирани со воден екстракт од GT го инхибираат растот на S. aureus и E. coli, додека
полиакрилонитрилот и памукот соодветно го инхибираат растот на E. faecalis и C. albicans.
Широки инхибициони зони за S. aureus се воочени за ткаенините функционализирани со BT.
Општо, испитуваните ткаенини покажуваат многу висока (81,60 – 100 %) способност за
отстранување на ABTS-радикали. Утврдена е добра линеарна зависност помеѓу TPC во водните
екстракти и антиоксидациската активност на волната и полиакрилонитрилот одредени преку
методот DPPH. Ткаенините со различен хемиски состав обоени и/или функционализирани со
водни екстракти од GT или BT можат да се користат за производство на медицински текстил со
висока додадена вредност, кој има терапевтски, профилактички и заштитни функции.
Медицинските текстилни материјали имаат потенцијална примена за третман на рани, особено за
рани на кожа кои се подложни на инфекција со S. aureus. Покрај тоа, волната и памукот
функционализирани со водни екстракти од GT или BT можат да се користат за производство на
медицински завои и гази за еднократна употреба.
PB  - Macedonian Journal of Chemistry and Chemical Engineering
T2  - Macedonian Journal of Chemistry and Chemical Engineering
T1  - Characterization of tea aqueous extracts and their utilization for dyeing and functionalizing fabrics of different chemical compositions
T1  - Карактеризација на водни екстракти од чаеви и нивна употреба за боење и функционализација на ткаенини со различен хемиски состав
VL  - 42
IS  - 2
SP  - 263
EP  - 273
DO  - 10.20450/mjcce2023.2698
ER  - 
@article{
author = "Pavun, Leposava and Spasojević, Danijela and Ivanovska, Aleksandra and Lađarević, Jelena and Milenković, Marina and Uskoković-Marković, Snežana",
year = "2023",
abstract = "Green, black, rooibos, and hibiscus tea (GT, BT, RT, and HT) aqueous extracts were prepared and
characterized in terms of total flavonoids (TFC) and total phenolic (TPC) contents and antioxidant and
antimicrobial activities. BT has the highest, while HT has the lowest TFC (1213 vs. 415 mg l–1
), while the
extracts’ TPCs (2283 – 7251 mg l–1
) decreased in the following order: BT > GT > RT > HT. Their antioxidant activities of 78.1 – 93.1% and 97.8 – 100% were determined according to DPPH and ABTS methods, respectively. BT and especially GT aqueous extracts possessed mild effects against several microorganisms. All examined extracts have an affinity for dyeing wool, cellulose acetate, polyamide, and cotton,
which is proven by the color strength values of 1.65–19.12. Wool, polyacrylonitrile, polyester, polyamide, cotton, and cellulose acetate functionalized with GT aqueous extract inhibited the growth of S. aureus
and E. coli, while polyacrylonitrile and cotton also inhibited the growth of E. faecalis, and C. albicans,
respectively. Wide inhibition zones for S. aureus were observed for fabrics functionalized with BT aqueous extract. Generally, the investigated fabrics showed very high (81.60 – 100%) ABTS radical scavenging ability independent of the extract used. TPCs have good linear correlations with the antioxidant activities of wool and polyacrylonitrile determined by the DPPH method. Fabrics with different chemical compositions dyed and/or functionalized with GT or BT aqueous extracts can be used to produce high-valueadded medical textiles with therapeutic, prophylactic, and protective functions. They can find potential applications in wound treatment, especially in skin wounds that are susceptible to infection with S. aureus.
Moreover, wool and cotton functionalized with GT or BT aqueous extract can also be considered for use in
disposable medical textiles like bandages and gauze used in the wound-healing process.
Keywords: tea aqueous extract; fabric; functionalization; dyeing; antioxidant and antimicrobial, Водните екстракти подготвени од црн, зелен, ројбуш и хибискус чај (GT, BT, RT и HT) се
карактеризирани од аспект на количество вкупни флавоноиди (TFC) и вкупни феноли (TPC), и
антиоксидациска и антимикробна активност. BT покажува најголема, а HT најмала TFC (1213 vs.
415 mg l–1), додека TPC во воденте екстракти (2283 – 7251 mg l–1) се намалува според следниов
редослед: BT > GT > RT > HT. Нивната антиоксидациска активност, одредена користејќи ги
методите DPPH и ABTS, се движи соодветно во опсег 78,1 – 93.1 % и 97,8 – 100 %. Водните
екстракти на BT и GT покажаа благ ефект врз одредени микроорганизми. Сите испитувани
екстракти имаат афинитет за боење волна, целулозен ацетат, полиамид и памук, што е потврдено
со одредување на јачина на бојата на ткаенините која изнесува 1,65 – 19.12. Волната,
полиакрилонитрилот, полиестерот, полиамидот, памукот и целулозниот ацетат функционализирани со воден екстракт од GT го инхибираат растот на S. aureus и E. coli, додека
полиакрилонитрилот и памукот соодветно го инхибираат растот на E. faecalis и C. albicans.
Широки инхибициони зони за S. aureus се воочени за ткаенините функционализирани со BT.
Општо, испитуваните ткаенини покажуваат многу висока (81,60 – 100 %) способност за
отстранување на ABTS-радикали. Утврдена е добра линеарна зависност помеѓу TPC во водните
екстракти и антиоксидациската активност на волната и полиакрилонитрилот одредени преку
методот DPPH. Ткаенините со различен хемиски состав обоени и/или функционализирани со
водни екстракти од GT или BT можат да се користат за производство на медицински текстил со
висока додадена вредност, кој има терапевтски, профилактички и заштитни функции.
Медицинските текстилни материјали имаат потенцијална примена за третман на рани, особено за
рани на кожа кои се подложни на инфекција со S. aureus. Покрај тоа, волната и памукот
функционализирани со водни екстракти од GT или BT можат да се користат за производство на
медицински завои и гази за еднократна употреба.",
publisher = "Macedonian Journal of Chemistry and Chemical Engineering",
journal = "Macedonian Journal of Chemistry and Chemical Engineering",
title = "Characterization of tea aqueous extracts and their utilization for dyeing and functionalizing fabrics of different chemical compositions, Карактеризација на водни екстракти од чаеви и нивна употреба за боење и функционализација на ткаенини со различен хемиски состав",
volume = "42",
number = "2",
pages = "263-273",
doi = "10.20450/mjcce2023.2698"
}
Pavun, L., Spasojević, D., Ivanovska, A., Lađarević, J., Milenković, M.,& Uskoković-Marković, S.. (2023). Characterization of tea aqueous extracts and their utilization for dyeing and functionalizing fabrics of different chemical compositions. in Macedonian Journal of Chemistry and Chemical Engineering
Macedonian Journal of Chemistry and Chemical Engineering., 42(2), 263-273.
https://doi.org/10.20450/mjcce2023.2698
Pavun L, Spasojević D, Ivanovska A, Lađarević J, Milenković M, Uskoković-Marković S. Characterization of tea aqueous extracts and their utilization for dyeing and functionalizing fabrics of different chemical compositions. in Macedonian Journal of Chemistry and Chemical Engineering. 2023;42(2):263-273.
doi:10.20450/mjcce2023.2698 .
Pavun, Leposava, Spasojević, Danijela, Ivanovska, Aleksandra, Lađarević, Jelena, Milenković, Marina, Uskoković-Marković, Snežana, "Characterization of tea aqueous extracts and their utilization for dyeing and functionalizing fabrics of different chemical compositions" in Macedonian Journal of Chemistry and Chemical Engineering, 42, no. 2 (2023):263-273,
https://doi.org/10.20450/mjcce2023.2698 . .

Reversed-phase thin-layer chromatographic and computational evaluation of lipophilicity parameters of α,β-unsaturated acids

Savić, Jelena; Vitnik, Vesna; Obradović, Darija; Vitnik, Željko; Petrić, Vanja; Čkalovski, Teodora; Lazović, Saša; Crevar, Milkica

(Springer, 2023)

TY  - JOUR
AU  - Savić, Jelena
AU  - Vitnik, Vesna
AU  - Obradović, Darija
AU  - Vitnik, Željko
AU  - Petrić, Vanja
AU  - Čkalovski, Teodora
AU  - Lazović, Saša
AU  - Crevar, Milkica
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5444
AB  - The retention behavior of 10 previously synthesized α,β-unsaturated acids that exhibited antimicrobial activity was studied using 12 reversed-phase thin-layer chromatography (RP-TLC) systems. The mobile phases consisted of three solvent combinations (methanol‒water, acetonitrile‒water, and acetone‒water) in four different ratios (50:50, 60:40, 70:30, and 80:20, V/V). The chromatographic parameters RM0 , a, and C0 were calculated for each system. The lipophilicity parameters of the tested compounds were predicted using various computational methods. The acetone‒water system demonstrated the highest correlation coefficients between the chromatographic and calculated lipophilicity parameters, which makes it the most suitable for evaluating the lipophilicity of the tested compounds. This system successfully reflected the effect of the lipophilic properties of the compounds on their retention behavior. To elucidate the retention mechanisms, the molecular properties of the tested compounds were calculated and a genetic algorithm was used to identify the properties with the greatest influence on the retention behavior. The interpretation of these descriptors revealed structural and physicochemical properties crucial for the behavior of the tested compounds. In addition, the pharmacokinetic properties of the compounds were estimated using in silico methods. The observed correlation between the retention mechanism and physicochemical properties affecting membrane transport and physiological binding ability highlights the applicability of RP-TLC conditions for rapid profiling of newly synthesized α,β-unsaturated acids.
PB  - Springer
T2  - Journal of Planar Chromatography - Modern TLC
T1  - Reversed-phase thin-layer chromatographic and computational evaluation of lipophilicity parameters of α,β-unsaturated acids
VL  - 36
IS  - 5
SP  - 415
EP  - 423
DO  - 10.1007/s00764-023-00274-9
ER  - 
@article{
author = "Savić, Jelena and Vitnik, Vesna and Obradović, Darija and Vitnik, Željko and Petrić, Vanja and Čkalovski, Teodora and Lazović, Saša and Crevar, Milkica",
year = "2023",
abstract = "The retention behavior of 10 previously synthesized α,β-unsaturated acids that exhibited antimicrobial activity was studied using 12 reversed-phase thin-layer chromatography (RP-TLC) systems. The mobile phases consisted of three solvent combinations (methanol‒water, acetonitrile‒water, and acetone‒water) in four different ratios (50:50, 60:40, 70:30, and 80:20, V/V). The chromatographic parameters RM0 , a, and C0 were calculated for each system. The lipophilicity parameters of the tested compounds were predicted using various computational methods. The acetone‒water system demonstrated the highest correlation coefficients between the chromatographic and calculated lipophilicity parameters, which makes it the most suitable for evaluating the lipophilicity of the tested compounds. This system successfully reflected the effect of the lipophilic properties of the compounds on their retention behavior. To elucidate the retention mechanisms, the molecular properties of the tested compounds were calculated and a genetic algorithm was used to identify the properties with the greatest influence on the retention behavior. The interpretation of these descriptors revealed structural and physicochemical properties crucial for the behavior of the tested compounds. In addition, the pharmacokinetic properties of the compounds were estimated using in silico methods. The observed correlation between the retention mechanism and physicochemical properties affecting membrane transport and physiological binding ability highlights the applicability of RP-TLC conditions for rapid profiling of newly synthesized α,β-unsaturated acids.",
publisher = "Springer",
journal = "Journal of Planar Chromatography - Modern TLC",
title = "Reversed-phase thin-layer chromatographic and computational evaluation of lipophilicity parameters of α,β-unsaturated acids",
volume = "36",
number = "5",
pages = "415-423",
doi = "10.1007/s00764-023-00274-9"
}
Savić, J., Vitnik, V., Obradović, D., Vitnik, Ž., Petrić, V., Čkalovski, T., Lazović, S.,& Crevar, M.. (2023). Reversed-phase thin-layer chromatographic and computational evaluation of lipophilicity parameters of α,β-unsaturated acids. in Journal of Planar Chromatography - Modern TLC
Springer., 36(5), 415-423.
https://doi.org/10.1007/s00764-023-00274-9
Savić J, Vitnik V, Obradović D, Vitnik Ž, Petrić V, Čkalovski T, Lazović S, Crevar M. Reversed-phase thin-layer chromatographic and computational evaluation of lipophilicity parameters of α,β-unsaturated acids. in Journal of Planar Chromatography - Modern TLC. 2023;36(5):415-423.
doi:10.1007/s00764-023-00274-9 .
Savić, Jelena, Vitnik, Vesna, Obradović, Darija, Vitnik, Željko, Petrić, Vanja, Čkalovski, Teodora, Lazović, Saša, Crevar, Milkica, "Reversed-phase thin-layer chromatographic and computational evaluation of lipophilicity parameters of α,β-unsaturated acids" in Journal of Planar Chromatography - Modern TLC, 36, no. 5 (2023):415-423,
https://doi.org/10.1007/s00764-023-00274-9 . .

Prolonged Zaleplon Treatment Increases the Expression of Proteins Involved in GABAergic and Glutamatergic Signaling in the Rat Hippocampus

Martinović, Jelena; Samardžić, Janko; Zarić Kontić, Marina; Ivković, Sanja; Dacić, Sanja; Major, Tamara; Radosavljević, Milica; Švob Štrac, Dubravka

(MDPI, 2023)

TY  - JOUR
AU  - Martinović, Jelena
AU  - Samardžić, Janko
AU  - Zarić Kontić, Marina
AU  - Ivković, Sanja
AU  - Dacić, Sanja
AU  - Major, Tamara
AU  - Radosavljević, Milica
AU  - Švob Štrac, Dubravka
PY  - 2023
UR  - https://farfar.pharmacy.bg.ac.rs/handle/123456789/5407
AB  - Zaleplon is a positive allosteric modulator of the γ-aminobutyric acid (GABA)A receptor approved for the short-term treatment of insomnia. Previous publications on zaleplon have not addressed the proteins involved in its mechanism of action but have mostly referred to behavioral or pharmacological studies. Since both GABAergic and glutamatergic signaling have been shown to regulate wakefulness and sleep, we examined the effects of prolonged zaleplon treatment (0.625 mg/kg for 5 days) on these systems in the hippocampus of male Wistar rats. Western blot and immunohistochemical analyses showed that the upregulated components of GABAergic signaling (glutamate decarboxylase, vesicular GABA transporter, GABA, and α1 subunit of the GABAA receptor) were accompanied by increased protein levels in the glutamatergic system (vesicular glutamate transporter 1 and NR1, NR2A, and NR2B subunits of N-methyl-d-aspartate receptor). Our results, showing that zaleplon enhances GABA neurotransmission in the hippocampus, were not surprising. However, we found that treatment also increased glutamatergic signaling. This could be the result of the downregulation of adenosine A1 receptors, important modulators of the glutamatergic system. Further studies are needed to investigate the effects of the zaleplon-induced increase in hippocampal glutamatergic neurotransmission and the possible involvement of the adenosine system in zaleplon’s mechanism of action.
PB  - MDPI
T2  - Brain Sciences
T1  - Prolonged Zaleplon Treatment Increases the Expression of Proteins Involved in GABAergic and Glutamatergic Signaling in the Rat Hippocampus
VL  - 13
IS  - 12
DO  - 10.3390/brainsci13121707
ER  - 
@article{
author = "Martinović, Jelena and Samardžić, Janko and Zarić Kontić, Marina and Ivković, Sanja and Dacić, Sanja and Major, Tamara and Radosavljević, Milica and Švob Štrac, Dubravka",
year = "2023",
abstract = "Zaleplon is a positive allosteric modulator of the γ-aminobutyric acid (GABA)A receptor approved for the short-term treatment of insomnia. Previous publications on zaleplon have not addressed the proteins involved in its mechanism of action but have mostly referred to behavioral or pharmacological studies. Since both GABAergic and glutamatergic signaling have been shown to regulate wakefulness and sleep, we examined the effects of prolonged zaleplon treatment (0.625 mg/kg for 5 days) on these systems in the hippocampus of male Wistar rats. Western blot and immunohistochemical analyses showed that the upregulated components of GABAergic signaling (glutamate decarboxylase, vesicular GABA transporter, GABA, and α1 subunit of the GABAA receptor) were accompanied by increased protein levels in the glutamatergic system (vesicular glutamate transporter 1 and NR1, NR2A, and NR2B subunits of N-methyl-d-aspartate receptor). Our results, showing that zaleplon enhances GABA neurotransmission in the hippocampus, were not surprising. However, we found that treatment also increased glutamatergic signaling. This could be the result of the downregulation of adenosine A1 receptors, important modulators of the glutamatergic system. Further studies are needed to investigate the effects of the zaleplon-induced increase in hippocampal glutamatergic neurotransmission and the possible involvement of the adenosine system in zaleplon’s mechanism of action.",
publisher = "MDPI",
journal = "Brain Sciences",
title = "Prolonged Zaleplon Treatment Increases the Expression of Proteins Involved in GABAergic and Glutamatergic Signaling in the Rat Hippocampus",
volume = "13",
number = "12",
doi = "10.3390/brainsci13121707"
}
Martinović, J., Samardžić, J., Zarić Kontić, M., Ivković, S., Dacić, S., Major, T., Radosavljević, M.,& Švob Štrac, D.. (2023). Prolonged Zaleplon Treatment Increases the Expression of Proteins Involved in GABAergic and Glutamatergic Signaling in the Rat Hippocampus. in Brain Sciences
MDPI., 13(12).
https://doi.org/10.3390/brainsci13121707
Martinović J, Samardžić J, Zarić Kontić M, Ivković S, Dacić S, Major T, Radosavljević M, Švob Štrac D. Prolonged Zaleplon Treatment Increases the Expression of Proteins Involved in GABAergic and Glutamatergic Signaling in the Rat Hippocampus. in Brain Sciences. 2023;13(12).
doi:10.3390/brainsci13121707 .
Martinović, Jelena, Samardžić, Janko, Zarić Kontić, Marina, Ivković, Sanja, Dacić, Sanja, Major, Tamara, Radosavljević, Milica, Švob Štrac, Dubravka, "Prolonged Zaleplon Treatment Increases the Expression of Proteins Involved in GABAergic and Glutamatergic Signaling in the Rat Hippocampus" in Brain Sciences, 13, no. 12 (2023),
https://doi.org/10.3390/brainsci13121707 . .