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Protective effects of tungstophosphoric acid and sodium tungstate on chemically induced liver necrosis in wistar rats

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Authors
Uskoković-Marković, Snežana
Milenković, Marina
Topić, Aleksandra
Kotur-Stevuljević, Jelena
Stefanović, Aleksandra
Antić-Stanković, Jelena
Article (Published version)
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Abstract
Purpose. Many chemical compounds and infectious agents such as viruses induce liver damage like necrosis or fulminant hepatic failure which is sometimes difficult to manage by medical therapies. The induced liver necrosis by carbon tetrachloride (CCl4) and thioacetamide (TAA) are exemplary models for experimental liver necrosis caused by oxygen free radicals. The aim of this study was to investigate the effects of tungstophosphoric acid (TPA) and sodium tungstate (ST) on liver injury induced by CCl4 or TAA. Methods. Hepatoprotective effects of TPA and ST on acute liver necrosis, chemically induced, were evaluated by the activity of serum enzymes (alkaline phosphatase, alanine transaminase and aspartate transaminase), oxidative stress parameters (activity of xanthine oxidase, concentrations of malondialdehyde and production of superoxide anion), antioxidative defence markers (concentration of reduced glutathione), and histopathology in Wistar rats. Liver necrosis was induced by administ...ering a single intraperitoneal (i.p.) injection of CCl4 (1.0 ml/kg b.wt. of 80% CCl4 in corn oil) or a single i.p. injection of TAA (400 mg/kg b.w. dissolved in normal saline). TPA and ST were administrated to rats orally for 7 weeks (50 mg/kg b.wt.) prior to induction of liver necrosis. Results. Induced liver necrosis caused significant elevation of activity of liver enzymes, parameters of oxidative stress and marked changes in histopathology, like necrosis of hepatocytes, hepatocyte degeneration and infiltration of inflammatory cells. In TPA and ST pretreated rats histopathological changes were almost absent, serum enzymes and oxidative stress parameters were decreased, while at the same time the concentration of reduced gluthathione was increased. Conclusion. The present findings suggest that treatment with TPA and ST for 7 weeks could be useful for the prevention of hepatic injury in rats.

Source:
Journal of Pharmacy and Pharmaceutical Sciences, 2007, 10, 3, 340-349
Publisher:
  • Canadian Society For Pharmaceutical Sciences

ISSN: 1482-1826

WoS: 000253451600009

Scopus: 2-s2.0-34848897541
[ Google Scholar ]
37
28
URI
http://farfar.pharmacy.bg.ac.rs/handle/123456789/1000
Collections
  • Radovi istraživača / Researchers’ publications
Institution
Pharmacy
TY  - JOUR
AU  - Uskoković-Marković, Snežana
AU  - Milenković, Marina
AU  - Topić, Aleksandra
AU  - Kotur-Stevuljević, Jelena
AU  - Stefanović, Aleksandra
AU  - Antić-Stanković, Jelena
PY  - 2007
UR  - http://farfar.pharmacy.bg.ac.rs/handle/123456789/1000
AB  - Purpose. Many chemical compounds and infectious agents such as viruses induce liver damage like necrosis or fulminant hepatic failure which is sometimes difficult to manage by medical therapies. The induced liver necrosis by carbon tetrachloride (CCl4) and thioacetamide (TAA) are exemplary models for experimental liver necrosis caused by oxygen free radicals. The aim of this study was to investigate the effects of tungstophosphoric acid (TPA) and sodium tungstate (ST) on liver injury induced by CCl4 or TAA. Methods. Hepatoprotective effects of TPA and ST on acute liver necrosis, chemically induced, were evaluated by the activity of serum enzymes (alkaline phosphatase, alanine transaminase and aspartate transaminase), oxidative stress parameters (activity of xanthine oxidase, concentrations of malondialdehyde and production of superoxide anion), antioxidative defence markers (concentration of reduced glutathione), and histopathology in Wistar rats. Liver necrosis was induced by administering a single intraperitoneal (i.p.) injection of CCl4 (1.0 ml/kg b.wt. of 80% CCl4 in corn oil) or a single i.p. injection of TAA (400 mg/kg b.w. dissolved in normal saline). TPA and ST were administrated to rats orally for 7 weeks (50 mg/kg b.wt.) prior to induction of liver necrosis. Results. Induced liver necrosis caused significant elevation of activity of liver enzymes, parameters of oxidative stress and marked changes in histopathology, like necrosis of hepatocytes, hepatocyte degeneration and infiltration of inflammatory cells. In TPA and ST pretreated rats histopathological changes were almost absent, serum enzymes and oxidative stress parameters were decreased, while at the same time the concentration of reduced gluthathione was increased. Conclusion. The present findings suggest that treatment with TPA and ST for 7 weeks could be useful for the prevention of hepatic injury in rats.
PB  - Canadian Society For Pharmaceutical Sciences
T2  - Journal of Pharmacy and Pharmaceutical Sciences
T1  - Protective effects of tungstophosphoric acid and sodium tungstate on chemically induced liver necrosis in wistar rats
VL  - 10
IS  - 3
SP  - 340
EP  - 349
ER  - 
@article{
author = "Uskoković-Marković, Snežana and Milenković, Marina and Topić, Aleksandra and Kotur-Stevuljević, Jelena and Stefanović, Aleksandra and Antić-Stanković, Jelena",
year = "2007",
url = "http://farfar.pharmacy.bg.ac.rs/handle/123456789/1000",
abstract = "Purpose. Many chemical compounds and infectious agents such as viruses induce liver damage like necrosis or fulminant hepatic failure which is sometimes difficult to manage by medical therapies. The induced liver necrosis by carbon tetrachloride (CCl4) and thioacetamide (TAA) are exemplary models for experimental liver necrosis caused by oxygen free radicals. The aim of this study was to investigate the effects of tungstophosphoric acid (TPA) and sodium tungstate (ST) on liver injury induced by CCl4 or TAA. Methods. Hepatoprotective effects of TPA and ST on acute liver necrosis, chemically induced, were evaluated by the activity of serum enzymes (alkaline phosphatase, alanine transaminase and aspartate transaminase), oxidative stress parameters (activity of xanthine oxidase, concentrations of malondialdehyde and production of superoxide anion), antioxidative defence markers (concentration of reduced glutathione), and histopathology in Wistar rats. Liver necrosis was induced by administering a single intraperitoneal (i.p.) injection of CCl4 (1.0 ml/kg b.wt. of 80% CCl4 in corn oil) or a single i.p. injection of TAA (400 mg/kg b.w. dissolved in normal saline). TPA and ST were administrated to rats orally for 7 weeks (50 mg/kg b.wt.) prior to induction of liver necrosis. Results. Induced liver necrosis caused significant elevation of activity of liver enzymes, parameters of oxidative stress and marked changes in histopathology, like necrosis of hepatocytes, hepatocyte degeneration and infiltration of inflammatory cells. In TPA and ST pretreated rats histopathological changes were almost absent, serum enzymes and oxidative stress parameters were decreased, while at the same time the concentration of reduced gluthathione was increased. Conclusion. The present findings suggest that treatment with TPA and ST for 7 weeks could be useful for the prevention of hepatic injury in rats.",
publisher = "Canadian Society For Pharmaceutical Sciences",
journal = "Journal of Pharmacy and Pharmaceutical Sciences",
title = "Protective effects of tungstophosphoric acid and sodium tungstate on chemically induced liver necrosis in wistar rats",
volume = "10",
number = "3",
pages = "340-349"
}
Uskoković-Marković S, Milenković M, Topić A, Kotur-Stevuljević J, Stefanović A, Antić-Stanković J. Protective effects of tungstophosphoric acid and sodium tungstate on chemically induced liver necrosis in wistar rats. Journal of Pharmacy and Pharmaceutical Sciences. 2007;10(3):340-349
Uskoković-Marković, S., Milenković, M., Topić, A., Kotur-Stevuljević, J., Stefanović, A.,& Antić-Stanković, J. (2007). Protective effects of tungstophosphoric acid and sodium tungstate on chemically induced liver necrosis in wistar rats.
Journal of Pharmacy and Pharmaceutical SciencesCanadian Society For Pharmaceutical Sciences., 10(3), 340-349.
Uskoković-Marković Snežana, Milenković Marina, Topić Aleksandra, Kotur-Stevuljević Jelena, Stefanović Aleksandra, Antić-Stanković Jelena, "Protective effects of tungstophosphoric acid and sodium tungstate on chemically induced liver necrosis in wistar rats" 10, no. 3 (2007):340-349

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