A spectrophotometric method is described for the assay of thioctic acid. The method is based on the reaction between the drug and palladium(II) chloride. In this reaction, a yellow-colored, water soluble product with a 1:1 stoichiometric ratio and an absorption maximum at 365 nm was formed. The stability of the formed complex depends on various factors (pH, reaction time, concentration of reagents, ionic strength). Based on these findings, a new method is suggested for the spectrophotometric determination of thioctic acid in pharmaceutical formulations. This method is simple, sensitive and reproducible.
Opisana je jednostavna spektrofotometrijska metoda za brzo i tačno određivanje tioktinske kiseline u vodi, injekcionim rastvorima i tabletama. Spektrofotometrijska merenja su izvođena u Britton-Robinson-ovim puferima pri različitim pH vrednostima (2,00-6,00) i konstantnoj jonskoj sili. Sastav, konstanta stabilnosti, molarna apsorptivnost kompleksa su određene različitim metodama. Utvrđen je 1:1 sastav nagrađenog kompleksa i molarna apsorptivnost 4.2x103 l mol-1 cm-1. Relativna standardna devijacija iznosi od 3,10 do 3,76%,{to odgovara propisanim analitičkim standardima.
Keywords:
thioctic acid / spectrophotometric determination / injection solutions and tablets
Source:
Journal of the Serbian Chemical Society, 2007, 72, 1, 29-35
TY - JOUR
AU - Korićanac, Zagorka
AU - Čakar, Mira
AU - Tanasković, Slađana
AU - Jovanović, Tatijana
PY - 2007
UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1003
AB - A spectrophotometric method is described for the assay of thioctic acid. The method is based on the reaction between the drug and palladium(II) chloride. In this reaction, a yellow-colored, water soluble product with a 1:1 stoichiometric ratio and an absorption maximum at 365 nm was formed. The stability of the formed complex depends on various factors (pH, reaction time, concentration of reagents, ionic strength). Based on these findings, a new method is suggested for the spectrophotometric determination of thioctic acid in pharmaceutical formulations. This method is simple, sensitive and reproducible.
AB - Opisana je jednostavna spektrofotometrijska metoda za brzo i tačno određivanje tioktinske kiseline u vodi, injekcionim rastvorima i tabletama. Spektrofotometrijska merenja su izvođena u Britton-Robinson-ovim puferima pri različitim pH vrednostima (2,00-6,00) i konstantnoj jonskoj sili. Sastav, konstanta stabilnosti, molarna apsorptivnost kompleksa su određene različitim metodama. Utvrđen je 1:1 sastav nagrađenog kompleksa i molarna apsorptivnost 4.2x103 l mol-1 cm-1. Relativna standardna devijacija iznosi od 3,10 do 3,76%,{to odgovara propisanim analitičkim standardima.
PB - Srpsko hemijsko društvo, Beograd
T2 - Journal of the Serbian Chemical Society
T1 - Spectrophotometric determination of thioctic (α-lipoic) acid in water and pharmaceutical preparations
T1 - Spektrofotometrijsko određivanje tioktinske kiseline u vodi i farmaceutskim preparatima
VL - 72
IS - 1
SP - 29
EP - 35
DO - 10.2298/JSC0701029K
ER -
@article{
author = "Korićanac, Zagorka and Čakar, Mira and Tanasković, Slađana and Jovanović, Tatijana",
year = "2007",
abstract = "A spectrophotometric method is described for the assay of thioctic acid. The method is based on the reaction between the drug and palladium(II) chloride. In this reaction, a yellow-colored, water soluble product with a 1:1 stoichiometric ratio and an absorption maximum at 365 nm was formed. The stability of the formed complex depends on various factors (pH, reaction time, concentration of reagents, ionic strength). Based on these findings, a new method is suggested for the spectrophotometric determination of thioctic acid in pharmaceutical formulations. This method is simple, sensitive and reproducible., Opisana je jednostavna spektrofotometrijska metoda za brzo i tačno određivanje tioktinske kiseline u vodi, injekcionim rastvorima i tabletama. Spektrofotometrijska merenja su izvođena u Britton-Robinson-ovim puferima pri različitim pH vrednostima (2,00-6,00) i konstantnoj jonskoj sili. Sastav, konstanta stabilnosti, molarna apsorptivnost kompleksa su određene različitim metodama. Utvrđen je 1:1 sastav nagrađenog kompleksa i molarna apsorptivnost 4.2x103 l mol-1 cm-1. Relativna standardna devijacija iznosi od 3,10 do 3,76%,{to odgovara propisanim analitičkim standardima.",
publisher = "Srpsko hemijsko društvo, Beograd",
journal = "Journal of the Serbian Chemical Society",
title = "Spectrophotometric determination of thioctic (α-lipoic) acid in water and pharmaceutical preparations, Spektrofotometrijsko određivanje tioktinske kiseline u vodi i farmaceutskim preparatima",
volume = "72",
number = "1",
pages = "29-35",
doi = "10.2298/JSC0701029K"
}
Korićanac, Z., Čakar, M., Tanasković, S.,& Jovanović, T.. (2007). Spectrophotometric determination of thioctic (α-lipoic) acid in water and pharmaceutical preparations. in Journal of the Serbian Chemical Society
Srpsko hemijsko društvo, Beograd., 72(1), 29-35.
https://doi.org/10.2298/JSC0701029K
Korićanac Z, Čakar M, Tanasković S, Jovanović T. Spectrophotometric determination of thioctic (α-lipoic) acid in water and pharmaceutical preparations. in Journal of the Serbian Chemical Society. 2007;72(1):29-35.
doi:10.2298/JSC0701029K .
Korićanac, Zagorka, Čakar, Mira, Tanasković, Slađana, Jovanović, Tatijana, "Spectrophotometric determination of thioctic (α-lipoic) acid in water and pharmaceutical preparations" in Journal of the Serbian Chemical Society, 72, no. 1 (2007):29-35,
https://doi.org/10.2298/JSC0701029K . .
