We propose a simple and precise densitometric method for measuring vanillylmandelic acid (VMA). The method comprises direct urine application, thin-layer chromatographic (TLC) separation, postchromatographic derivatization, and in situ reading of the formed derivative at 560 nm. Quantitation was done by measuring peak area with a computer-controlled TLC Scanner and applying a five-point calibration function. The calibration function was 7.57-49.23 μmol/L. This method was used to determine urinary concentrations of excreted VMA; concentrations ranged from 4.84 to 56.06 μmol/day.
Keywords:
in situ post-chromatographic derivatization / thin-layer chromatography
TY - JOUR
AU - Agbaba, Danica
AU - Stojanov, Marina
AU - Rajacić, S
AU - Živanov-Stakić, Dobrila
AU - Majkić-Singh, Nada
PY - 1993
UR - http://farfar.pharmacy.bg.ac.rs/handle/123456789/100
AB - We propose a simple and precise densitometric method for measuring vanillylmandelic acid (VMA). The method comprises direct urine application, thin-layer chromatographic (TLC) separation, postchromatographic derivatization, and in situ reading of the formed derivative at 560 nm. Quantitation was done by measuring peak area with a computer-controlled TLC Scanner and applying a five-point calibration function. The calibration function was 7.57-49.23 μmol/L. This method was used to determine urinary concentrations of excreted VMA; concentrations ranged from 4.84 to 56.06 μmol/day.
T2 - Clinical Chemistry
T1 - Densitometric determination of urinary 4-hydroxy-3-methoxymandelic acid (vanillylmandelic acid)
VL - 39
IS - 12
SP - 2500
EP - 2503
ER -
@article{
author = "Agbaba, Danica and Stojanov, Marina and Rajacić, S and Živanov-Stakić, Dobrila and Majkić-Singh, Nada",
year = "1993",
url = "http://farfar.pharmacy.bg.ac.rs/handle/123456789/100",
abstract = "We propose a simple and precise densitometric method for measuring vanillylmandelic acid (VMA). The method comprises direct urine application, thin-layer chromatographic (TLC) separation, postchromatographic derivatization, and in situ reading of the formed derivative at 560 nm. Quantitation was done by measuring peak area with a computer-controlled TLC Scanner and applying a five-point calibration function. The calibration function was 7.57-49.23 μmol/L. This method was used to determine urinary concentrations of excreted VMA; concentrations ranged from 4.84 to 56.06 μmol/day.",
journal = "Clinical Chemistry",
title = "Densitometric determination of urinary 4-hydroxy-3-methoxymandelic acid (vanillylmandelic acid)",
volume = "39",
number = "12",
pages = "2500-2503"
}
Agbaba D, Stojanov M, Rajacić S, Živanov-Stakić D, Majkić-Singh N. Densitometric determination of urinary 4-hydroxy-3-methoxymandelic acid (vanillylmandelic acid). Clinical Chemistry. 1993;39(12):2500-2503
Agbaba, D., Stojanov, M., Rajacić, S., Živanov-Stakić, D.,& Majkić-Singh, N. (1993). Densitometric determination of urinary 4-hydroxy-3-methoxymandelic acid (vanillylmandelic acid).
Clinical Chemistry, 39(12), 2500-2503.
