Densitometric determination of urinary 4-hydroxy-3-methoxymandelic acid (vanillylmandelic acid)
Апстракт
We propose a simple and precise densitometric method for measuring vanillylmandelic acid (VMA). The method comprises direct urine application, thin-layer chromatographic (TLC) separation, postchromatographic derivatization, and in situ reading of the formed derivative at 560 nm. Quantitation was done by measuring peak area with a computer-controlled TLC Scanner and applying a five-point calibration function. The calibration function was 7.57-49.23 μmol/L. This method was used to determine urinary concentrations of excreted VMA; concentrations ranged from 4.84 to 56.06 μmol/day.
Кључне речи:
in situ post-chromatographic derivatization / thin-layer chromatographyИзвор:
Clinical Chemistry, 1993, 39, 12, 2500-2503Институција/група
PharmacyTY - JOUR AU - Agbaba, Danica AU - Stojanov, Marina AU - Rajacić, S AU - Živanov-Stakić, Dobrila AU - Majkić-Singh, Nada PY - 1993 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/100 AB - We propose a simple and precise densitometric method for measuring vanillylmandelic acid (VMA). The method comprises direct urine application, thin-layer chromatographic (TLC) separation, postchromatographic derivatization, and in situ reading of the formed derivative at 560 nm. Quantitation was done by measuring peak area with a computer-controlled TLC Scanner and applying a five-point calibration function. The calibration function was 7.57-49.23 μmol/L. This method was used to determine urinary concentrations of excreted VMA; concentrations ranged from 4.84 to 56.06 μmol/day. T2 - Clinical Chemistry T1 - Densitometric determination of urinary 4-hydroxy-3-methoxymandelic acid (vanillylmandelic acid) VL - 39 IS - 12 SP - 2500 EP - 2503 UR - https://hdl.handle.net/21.15107/rcub_farfar_100 ER -
@article{ author = "Agbaba, Danica and Stojanov, Marina and Rajacić, S and Živanov-Stakić, Dobrila and Majkić-Singh, Nada", year = "1993", abstract = "We propose a simple and precise densitometric method for measuring vanillylmandelic acid (VMA). The method comprises direct urine application, thin-layer chromatographic (TLC) separation, postchromatographic derivatization, and in situ reading of the formed derivative at 560 nm. Quantitation was done by measuring peak area with a computer-controlled TLC Scanner and applying a five-point calibration function. The calibration function was 7.57-49.23 μmol/L. This method was used to determine urinary concentrations of excreted VMA; concentrations ranged from 4.84 to 56.06 μmol/day.", journal = "Clinical Chemistry", title = "Densitometric determination of urinary 4-hydroxy-3-methoxymandelic acid (vanillylmandelic acid)", volume = "39", number = "12", pages = "2500-2503", url = "https://hdl.handle.net/21.15107/rcub_farfar_100" }
Agbaba, D., Stojanov, M., Rajacić, S., Živanov-Stakić, D.,& Majkić-Singh, N.. (1993). Densitometric determination of urinary 4-hydroxy-3-methoxymandelic acid (vanillylmandelic acid). in Clinical Chemistry, 39(12), 2500-2503. https://hdl.handle.net/21.15107/rcub_farfar_100
Agbaba D, Stojanov M, Rajacić S, Živanov-Stakić D, Majkić-Singh N. Densitometric determination of urinary 4-hydroxy-3-methoxymandelic acid (vanillylmandelic acid). in Clinical Chemistry. 1993;39(12):2500-2503. https://hdl.handle.net/21.15107/rcub_farfar_100 .
Agbaba, Danica, Stojanov, Marina, Rajacić, S, Živanov-Stakić, Dobrila, Majkić-Singh, Nada, "Densitometric determination of urinary 4-hydroxy-3-methoxymandelic acid (vanillylmandelic acid)" in Clinical Chemistry, 39, no. 12 (1993):2500-2503, https://hdl.handle.net/21.15107/rcub_farfar_100 .