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Densitometric determination of urinary 4-hydroxy-3-methoxymandelic acid (vanillylmandelic acid)
dc.creator | Agbaba, Danica | |
dc.creator | Stojanov, Marina | |
dc.creator | Rajacić, S | |
dc.creator | Živanov-Stakić, Dobrila | |
dc.creator | Majkić-Singh, Nada | |
dc.date.accessioned | 2019-09-02T10:48:27Z | |
dc.date.available | 2019-09-02T10:48:27Z | |
dc.date.issued | 1993 | |
dc.identifier.issn | 0009-9147 | |
dc.identifier.uri | https://farfar.pharmacy.bg.ac.rs/handle/123456789/100 | |
dc.description.abstract | We propose a simple and precise densitometric method for measuring vanillylmandelic acid (VMA). The method comprises direct urine application, thin-layer chromatographic (TLC) separation, postchromatographic derivatization, and in situ reading of the formed derivative at 560 nm. Quantitation was done by measuring peak area with a computer-controlled TLC Scanner and applying a five-point calibration function. The calibration function was 7.57-49.23 μmol/L. This method was used to determine urinary concentrations of excreted VMA; concentrations ranged from 4.84 to 56.06 μmol/day. | en |
dc.rights | restrictedAccess | |
dc.source | Clinical Chemistry | |
dc.subject | in situ post-chromatographic derivatization | en |
dc.subject | thin-layer chromatography | en |
dc.title | Densitometric determination of urinary 4-hydroxy-3-methoxymandelic acid (vanillylmandelic acid) | en |
dc.type | article | |
dc.rights.license | ARR | |
dcterms.abstract | Живанов-Стакић, Добрила; Aгбаба, Даница; Мајкић-Сингх, Нада; Рајацић, С; Стојанов, Марина; | |
dc.citation.volume | 39 | |
dc.citation.issue | 12 | |
dc.citation.spage | 2500 | |
dc.citation.epage | 2503 | |
dc.citation.other | 39(12): 2500-2503 | |
dc.identifier.scopus | 2-s2.0-0027732190 | |
dc.identifier.rcub | https://hdl.handle.net/21.15107/rcub_farfar_100 | |
dc.type.version | publishedVersion |
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