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Simultaneous determination of cefotaxime and desacetylcefotaxime in real urine sample using voltammetric and high-performance liquid chromatographic methods

Authorized Users Only
2008
Authors
Aleksić, Mara
Kapetanović, Vera
Atanacković, Jasmina
Jocić, Biljana
Zečević, Mira
Article (Published version)
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Abstract
Two rapid, accurate and sensitive methods are developed and validated for the quantitative simultaneous determination of cefotaxime (CFX) and its active metabolite desacetylcefotaxime (DCFX) in urine. Based on the previous results which showed the four electron reduction of CFX at approximate to -0.5 V, and the new findings that DCFX reduction occurred at more positive potential (-0.23 V), the new adsorptive stripping differential pulse voltammetric (AdSDPV) method was developed for determination of CFX in the presence of DCFX. Linear responses were observed over a wide concentration range (0.07-0.52 mu g/ml for CFX and 0.22-1.3 mu g/ml for DCFX) in urine. The second assay involves subsequent separation on a reversed-phase HPLC column, with ultraviolet detection at 262 nm. Retention times were 4.057 and 1.960 min for CFX and DCFX, respectively. Linear responses were observed over a wide range, 0.55-6.60 mu g/ml for CFX and 1.10-11.00 mu g/ml for DCFX, in urine. The statistical evaluati...on for both methods was examined by means of within-day repeatability (n=5) and day-to-day precision (n=3) and was found to be satisfactory with high accuracy and precision.

Keywords:
Cefotaxime / Desacetylcefotaxime / Simultaneous determination / Real urine / Voltammetry / RP-HPLC
Source:
Talanta, 2008, 77, 1, 131-137
Publisher:
  • Elsevier Science BV, Amsterdam
Projects:
  • Sinteza, kvantitativni odnosi između strukture/osobina i aktivnosti, fizičko-hemijska karakterizacija i analiza farmakološki aktivnih supstanci (RS-142071)

DOI: 10.1016/j.talanta.2008.05.047

ISSN: 0039-9140

PubMed: 18804610

WoS: 000260290200020

Scopus: 2-s2.0-51749107978
[ Google Scholar ]
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19
URI
http://farfar.pharmacy.bg.ac.rs/handle/123456789/1032
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  • Radovi istraživača / Researchers’ publications
Institution
Pharmacy

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