Приказ основних података о документу

dc.creatorAleksić, Mara
dc.creatorKapetanović, Vera
dc.creatorAtanacković, Jasmina
dc.creatorJocić, Biljana
dc.creatorZečević, Mira
dc.date.accessioned2019-09-02T11:12:23Z
dc.date.available2019-09-02T11:12:23Z
dc.date.issued2008
dc.identifier.issn0039-9140
dc.identifier.urihttps://farfar.pharmacy.bg.ac.rs/handle/123456789/1032
dc.description.abstractTwo rapid, accurate and sensitive methods are developed and validated for the quantitative simultaneous determination of cefotaxime (CFX) and its active metabolite desacetylcefotaxime (DCFX) in urine. Based on the previous results which showed the four electron reduction of CFX at approximate to -0.5 V, and the new findings that DCFX reduction occurred at more positive potential (-0.23 V), the new adsorptive stripping differential pulse voltammetric (AdSDPV) method was developed for determination of CFX in the presence of DCFX. Linear responses were observed over a wide concentration range (0.07-0.52 mu g/ml for CFX and 0.22-1.3 mu g/ml for DCFX) in urine. The second assay involves subsequent separation on a reversed-phase HPLC column, with ultraviolet detection at 262 nm. Retention times were 4.057 and 1.960 min for CFX and DCFX, respectively. Linear responses were observed over a wide range, 0.55-6.60 mu g/ml for CFX and 1.10-11.00 mu g/ml for DCFX, in urine. The statistical evaluation for both methods was examined by means of within-day repeatability (n=5) and day-to-day precision (n=3) and was found to be satisfactory with high accuracy and precision.en
dc.publisherElsevier Science BV, Amsterdam
dc.relationinfo:eu-repo/grantAgreement/MESTD/MPN2006-2010/142071/RS//
dc.rightsrestrictedAccess
dc.sourceTalanta
dc.subjectCefotaximeen
dc.subjectDesacetylcefotaximeen
dc.subjectSimultaneous determinationen
dc.subjectReal urineen
dc.subjectVoltammetryen
dc.subjectRP-HPLCen
dc.titleSimultaneous determination of cefotaxime and desacetylcefotaxime in real urine sample using voltammetric and high-performance liquid chromatographic methodsen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractЈоцић, Биљана; Aлексић, Мара; Aтанацковић, Јасмина; Капетановић, Вера; Зечевић, Мира;
dc.citation.volume77
dc.citation.issue1
dc.citation.spage131
dc.citation.epage137
dc.citation.other77(1): 131-137
dc.citation.rankM21
dc.identifier.wos000260290200020
dc.identifier.doi10.1016/j.talanta.2008.05.047
dc.identifier.pmid18804610
dc.identifier.scopus2-s2.0-51749107978
dc.type.versionpublishedVersion


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Приказ основних података о документу