A sensitive spectrophotometric method for the determination of bifonazole is described. Bifonazole was treated with bromphenol blue (BPB) in the presence of citrate buffer (pH 2.5; μ = 0.23). The formed ion-pair complex was extracted with chloroform. By using spectrophotometric measurements, Job's and Bent-French's methods, it was found that bifonazole and BPB formed an ion-pair complex with the absorption maximum at 414 nm (molecular ratio of bifonazole BPB = 1:2) and molar absorptivity 2.23 x 104 L mol-1 cm-1. Beer's law was obeyed up to 2.5 μg ml-1. The proposed method was applied for determination of bifonazole in pharmaceutical formulations. Statistical analysis of the results obtained showed that the proposed method has good reproducibility and precision.
TY - JOUR
AU - Vladimirov, S
AU - Brborić, Jasmina
AU - Agbaba, Danica
AU - Zivanov, D
AU - Stakić, D.Ž
PY - 1993
UR - http://farfar.pharmacy.bg.ac.rs/handle/123456789/105
AB - A sensitive spectrophotometric method for the determination of bifonazole is described. Bifonazole was treated with bromphenol blue (BPB) in the presence of citrate buffer (pH 2.5; μ = 0.23). The formed ion-pair complex was extracted with chloroform. By using spectrophotometric measurements, Job's and Bent-French's methods, it was found that bifonazole and BPB formed an ion-pair complex with the absorption maximum at 414 nm (molecular ratio of bifonazole BPB = 1:2) and molar absorptivity 2.23 x 104 L mol-1 cm-1. Beer's law was obeyed up to 2.5 μg ml-1. The proposed method was applied for determination of bifonazole in pharmaceutical formulations. Statistical analysis of the results obtained showed that the proposed method has good reproducibility and precision.
T2 - Farmaco
T1 - Spectrophotometric determination of bifonazole in pharmaceutical formulations using bromphenol blue
VL - 48
IS - 7
SP - 1007
EP - 1015
ER -
@article{
author = "Vladimirov, S and Brborić, Jasmina and Agbaba, Danica and Zivanov, D and Stakić, D.Ž",
year = "1993",
url = "http://farfar.pharmacy.bg.ac.rs/handle/123456789/105",
abstract = "A sensitive spectrophotometric method for the determination of bifonazole is described. Bifonazole was treated with bromphenol blue (BPB) in the presence of citrate buffer (pH 2.5; μ = 0.23). The formed ion-pair complex was extracted with chloroform. By using spectrophotometric measurements, Job's and Bent-French's methods, it was found that bifonazole and BPB formed an ion-pair complex with the absorption maximum at 414 nm (molecular ratio of bifonazole BPB = 1:2) and molar absorptivity 2.23 x 104 L mol-1 cm-1. Beer's law was obeyed up to 2.5 μg ml-1. The proposed method was applied for determination of bifonazole in pharmaceutical formulations. Statistical analysis of the results obtained showed that the proposed method has good reproducibility and precision.",
journal = "Farmaco",
title = "Spectrophotometric determination of bifonazole in pharmaceutical formulations using bromphenol blue",
volume = "48",
number = "7",
pages = "1007-1015"
}
Vladimirov S, Brborić J, Agbaba D, Zivanov D, Stakić D. Spectrophotometric determination of bifonazole in pharmaceutical formulations using bromphenol blue. Farmaco. 1993;48(7):1007-1015
Vladimirov, S., Brborić, J., Agbaba, D., Zivanov, D.,& Stakić, D.Ž (1993). Spectrophotometric determination of bifonazole in pharmaceutical formulations using bromphenol blue.
Farmaco, 48(7), 1007-1015.
Vladimirov S, Brborić Jasmina, Agbaba Danica, Zivanov D, Stakić D.Ž, "Spectrophotometric determination of bifonazole in pharmaceutical formulations using bromphenol blue" 48, no. 7 (1993):1007-1015
