Microemulsion Liquid Chromatographic Screening of Simvastatin and its Active Metabolite in Human Plasma
Samo za registrovane korisnike
2008
Članak u časopisu (Objavljena verzija)
Metapodaci
Prikaz svih podataka o dokumentuApstrakt
This paper reports development and validation of a new microemulsion liquid chromatographic (MELC) method for rapid screening of simvastatin and simvastatin acid in human plasma. Plasma samples were injected directly into the HPLC system after appropriate sample dilution with mobile phase. Separations were performed on a 4.6 mm x 150 mm, 5-mu m particle, C-18 column, with UV detection at 238 nm. The mobile phase was 0.5% (w/v) diisopropyl ether, 1.0% (w/v) sodium dodecylsulphate (SDS), 4.0% (w/v) n-butanol, and 94.5% (w/w) aqueous 25 mM disodium hydrogen phosphate, pH 7.0, at a flow rate of 1 mL min(-1). The method was evaluated according to criteria stated in FDA bioanalytical method validation guidance. The unique approach applied in this paper enables direct analysis of simvastatin and simvastatin acid, so the method can be used to obtain reliable results in a rapid and simple way.
Ključne reči:
microemulsion liquid chromatography / human plasma / simvastatin / active metaboliteIzvor:
Acta Chromatographica, 2008, 20, 4, 595-607Izdavač:
- Akademiai Kiado Zrt, Budapest
Finansiranje / projekti:
DOI: 10.1556/AChrom.20.2008.4.6
ISSN: 1233-2356
WoS: 000261707900006
Scopus: 2-s2.0-70450233608
Institucija/grupa
PharmacyTY - JOUR AU - Malenović, Anđelija AU - Jančić-Stojanović, Biljana AU - Medenica, Mirjana AU - Ivanović, D. PY - 2008 UR - https://farfar.pharmacy.bg.ac.rs/handle/123456789/1058 AB - This paper reports development and validation of a new microemulsion liquid chromatographic (MELC) method for rapid screening of simvastatin and simvastatin acid in human plasma. Plasma samples were injected directly into the HPLC system after appropriate sample dilution with mobile phase. Separations were performed on a 4.6 mm x 150 mm, 5-mu m particle, C-18 column, with UV detection at 238 nm. The mobile phase was 0.5% (w/v) diisopropyl ether, 1.0% (w/v) sodium dodecylsulphate (SDS), 4.0% (w/v) n-butanol, and 94.5% (w/w) aqueous 25 mM disodium hydrogen phosphate, pH 7.0, at a flow rate of 1 mL min(-1). The method was evaluated according to criteria stated in FDA bioanalytical method validation guidance. The unique approach applied in this paper enables direct analysis of simvastatin and simvastatin acid, so the method can be used to obtain reliable results in a rapid and simple way. PB - Akademiai Kiado Zrt, Budapest T2 - Acta Chromatographica T1 - Microemulsion Liquid Chromatographic Screening of Simvastatin and its Active Metabolite in Human Plasma VL - 20 IS - 4 SP - 595 EP - 607 DO - 10.1556/AChrom.20.2008.4.6 ER -
@article{ author = "Malenović, Anđelija and Jančić-Stojanović, Biljana and Medenica, Mirjana and Ivanović, D.", year = "2008", abstract = "This paper reports development and validation of a new microemulsion liquid chromatographic (MELC) method for rapid screening of simvastatin and simvastatin acid in human plasma. Plasma samples were injected directly into the HPLC system after appropriate sample dilution with mobile phase. Separations were performed on a 4.6 mm x 150 mm, 5-mu m particle, C-18 column, with UV detection at 238 nm. The mobile phase was 0.5% (w/v) diisopropyl ether, 1.0% (w/v) sodium dodecylsulphate (SDS), 4.0% (w/v) n-butanol, and 94.5% (w/w) aqueous 25 mM disodium hydrogen phosphate, pH 7.0, at a flow rate of 1 mL min(-1). The method was evaluated according to criteria stated in FDA bioanalytical method validation guidance. The unique approach applied in this paper enables direct analysis of simvastatin and simvastatin acid, so the method can be used to obtain reliable results in a rapid and simple way.", publisher = "Akademiai Kiado Zrt, Budapest", journal = "Acta Chromatographica", title = "Microemulsion Liquid Chromatographic Screening of Simvastatin and its Active Metabolite in Human Plasma", volume = "20", number = "4", pages = "595-607", doi = "10.1556/AChrom.20.2008.4.6" }
Malenović, A., Jančić-Stojanović, B., Medenica, M.,& Ivanović, D.. (2008). Microemulsion Liquid Chromatographic Screening of Simvastatin and its Active Metabolite in Human Plasma. in Acta Chromatographica Akademiai Kiado Zrt, Budapest., 20(4), 595-607. https://doi.org/10.1556/AChrom.20.2008.4.6
Malenović A, Jančić-Stojanović B, Medenica M, Ivanović D. Microemulsion Liquid Chromatographic Screening of Simvastatin and its Active Metabolite in Human Plasma. in Acta Chromatographica. 2008;20(4):595-607. doi:10.1556/AChrom.20.2008.4.6 .
Malenović, Anđelija, Jančić-Stojanović, Biljana, Medenica, Mirjana, Ivanović, D., "Microemulsion Liquid Chromatographic Screening of Simvastatin and its Active Metabolite in Human Plasma" in Acta Chromatographica, 20, no. 4 (2008):595-607, https://doi.org/10.1556/AChrom.20.2008.4.6 . .