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dc.creatorInjac, Rade
dc.creatorKarljiković-Rajić, Katarina
dc.creatorŠtrukelj, Borut
dc.date.accessioned2019-09-02T11:13:09Z
dc.date.available2019-09-02T11:13:09Z
dc.date.issued2008
dc.identifier.issn0173-0835
dc.identifier.urihttp://farfar.pharmacy.bg.ac.rs/handle/123456789/1061
dc.description.abstractA novel and simple method has been developed for the determination of doxycycline (DOX) in biological fluids. The method is based on SPE, large-volume sample stacking (LVSS) and MEKC with UV-DAD detection. Six SPE cartridges have been used in investigation for sample clean up and pre-concentration (Supelco (R) LC-8, LC-18, LC-SCX, and LC-WCX, as well as Strata (TM)-X and X-C). DOX was determined on a 56 cm. (effective length 50 cm) x 50 mu m id fused-silica capillary. The BGE was 20 mM borate buffer, pH 9.3, containing 80 mM SDS and 7.5% v/v of methanol (30 s x 50 mbar), and the temperature and voltage were 25 degrees C and 30 kV, respectively. The analytical wavelength was set at 210 nm. Under optimized conditions it is possible to determine DOX in human serum, urine, semen, tears and saliva with recovery of 97.5% (RSD 2.5%). The method was shown to be sensitive (LOD is 1 mu g/L) and precise (intra-day RSD 0.2 and 2.4%; inter-days 0.4 and 3.5% for migration time and peak area, respectively). Results for developed SPE-LVSS-MEKC were compared with LVSS-MEKC method with direct sample injection. The new LVSS-MEKC method is presented as a useful technique for rapid determination without extraction procedure of DOX in human urine and serum, using 80mM of SDS, 10% v/v of methanol and 40 mM borate buffer (pH 9.3; 30 s x 50 mbar; 25 degrees C; 30 kV; 350 nm), but not for the other biological fluids, according to lower sensitivity of the method and because of the sample composition.en
dc.publisherWiley-VCH Verlag GMBH, Weinheim
dc.relationinfo:eu-repo/grantAgreement/MESTD/MPN2006-2010/142072/RS//
dc.rightsrestrictedAccess
dc.sourceElectrophoresis
dc.subjectBiological fluidsen
dc.subjectDirect injectionen
dc.subjectDoxycyclineen
dc.subjectLVSSen
dc.subjectSPE-MEKCen
dc.titleSPE and large-volume sample stacking in MEKC for determination of doxycycline in biological fluids: Comparison of direct injection to SPE-MEKCen
dc.typearticle
dc.rights.licenseARR
dcterms.abstractИњац, Раде; Карљиковић-Рајић, Катарина; Штрукељ, Борут;
dc.citation.volume29
dc.citation.issue21
dc.citation.spage4431
dc.citation.epage4438
dc.citation.other29(21): 4431-4438
dc.citation.rankaM21
dc.identifier.wos000261484100019
dc.identifier.doi10.1002/elps.200800339
dc.identifier.pmid18956436
dc.identifier.scopus2-s2.0-59249109357
dc.identifier.rcubconv_2094
dc.type.versionpublishedVersion


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